MC09g1435 (gene) Bitter gourd (Dali-11) v1

BLAST of MC09g1435 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 1.9e-77
Identity = 151/277 (54.51%), Postives = 182/277 (65.70%), Query Frame = 0

Query: 1   LLHSYKRSFNGFVVKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSQKVDRAQ 60
           +LH+YKRSFNGF VKLTE EA+K++ MEGV+SVF N   +LHTTRSWDF+GF   V R  
Sbjct: 69  VLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFPLTVPRRS 128

Query: 61  SVENDIIVGVIDSGIWPESPSFSD-GYGPPPAKWKGRCEVSANFSCNNKIIGARSYRSDG 120
            VE++I+VGV+D+GIWPESPSF D G+ PPP KWKG CE S NF CN KIIGARSY    
Sbjct: 129 QVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIGARSYHIGR 188

Query: 121 QLPEGDIKGPGDSDGHGTHTASTVAGGLVRGASMVGLGLGTARGSRGPFRAHRRALSAEI 180
            +  GD+ GP D++GHGTHTAST AGGLV  A++ GLGLGTARG        R A     
Sbjct: 189 PISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGG---VPLARIAAYKVC 248

Query: 181 WSD--------------------------------YYFNDYLATGSFHAINRGILSSLAV 240
           W+D                                +YF D +A GSFHA+ RGIL+S + 
Sbjct: 249 WNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTSNSA 308

Query: 241 GNNGPNFTTIVNFSPWSLSVAASSTDRKFVTRVELGN 245
           GN GPNF T  + SPW LSVAAS+ DRKFVT+V++GN
Sbjct: 309 GNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGN 342

BLAST of MC09g1435 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 7.1e-61
Identity = 130/273 (47.62%), Postives = 167/273 (61.17%), Query Frame = 0

Query: 1   LLHSYKRSFNGFVVKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSQKVDRAQ 60
           L+ SYKRSFNGF   L++AE++K+  M+ V+SVFP+   +L TTRSWDF+GF +K  R  
Sbjct: 68  LVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRES 127

Query: 61  SVENDIIVGVIDSGIWPESPSFSD-GYGPPPAKWKGRCEVSANFSCNNKIIGARSYRSDG 120
             E+D+IVGVIDSGIWPES SF D G+GPPP KWKG C+    F+CNNK+IGAR Y    
Sbjct: 128 VKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIGARFYNKFA 187

Query: 121 QLPEGDIKGPGDSDGHGTHTASTVAGGLVRGASMVGLGLGTARGSRGPFR--AHR----- 180
                      D +GHGTHTAST AG  V+ AS  GL  GTARG     R  A++     
Sbjct: 188 D-------SARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCFNR 247

Query: 181 ------------------RALSAEIWSDY---YFNDYLATGSFHAINRGILSSLAVGNNG 240
                               +S  I +DY     N  +A GSFHA+ RGI+++ + GNNG
Sbjct: 248 CNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNG 307

Query: 241 PNFTTIVNFSPWSLSVAASSTDRKFVTRVELGN 245
           P+  ++ N SPW ++VAAS TDR+F+ RV LGN
Sbjct: 308 PDQGSVANVSPWMITVAASGTDRQFIDRVVLGN 333

BLAST of MC09g1435 vs. ExPASy Swiss-Prot
Match: Q9FIM8 (Subtilisin-like protease SBT4.10 OS=Arabidopsis thaliana OX=3702 GN=SBT4.10 PE=5 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 2.3e-59
Identity = 133/276 (48.19%), Postives = 171/276 (61.96%), Query Frame = 0

Query: 1   LLHSYKRSFNGFVVKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSQ--KVDR 60
           L+ SYK+SFNGF  +LTE+E K+V+EMEGV+SVFP+ K +LHTT SWDFMG  +     R
Sbjct: 72  LVRSYKKSFNGFSARLTESERKRVAEMEGVVSVFPSKKYKLHTTASWDFMGLKEGKNTKR 131

Query: 61  AQSVENDIIVGVIDSGIWPESPSFS-DGYGPPPAKWKGRCEVSANFSCNNKIIGARSYRS 120
             +VE+D IVGV D+GI PES SFS  G+GPPP KWKG C+   NF+CNNK+IGAR Y +
Sbjct: 132 NLAVESDTIVGVFDTGISPESESFSGKGFGPPPKKWKGVCKGGKNFTCNNKLIGARDYTN 191

Query: 121 DGQLPEGDIKGPGDSDGHGTHTASTVAGGLVRGASMVGLGLGTARGSRGPFR--AHRRAL 180
           +         G  D +GHGTHTAST AG +V   S  G+G GTARG     R  A++   
Sbjct: 192 E---------GTRDIEGHGTHTASTAAGNVVENTSFYGIGNGTARGGVPDSRIAAYKVCS 251

Query: 181 SAEIWSDY---------------------------YFNDYLATGSFHAINRGILSSLAVG 240
            A   S+Y                           Y  D +A G+FHA+ +GIL+  + G
Sbjct: 252 GAGCSSEYILSAFDDAIADGVDVISASLGGDTAYMYEKDPIAIGAFHAMAKGILTVQSAG 311

Query: 241 NNGPNFTTIVNFSPWSLSVAASSTDRKFVTRVELGN 245
           NNGPN T  V+ +PW L+VAAS+T+R+ VT+V LGN
Sbjct: 312 NNGPNPT--VSVAPWILTVAASTTNRRIVTKVVLGN 336

BLAST of MC09g1435 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 2.3e-59
Identity = 132/276 (47.83%), Postives = 168/276 (60.87%), Query Frame = 0

Query: 1   LLHSYKRSFNGFVVKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSQ--KVDR 60
           L+ SYKRSFNGF  +LTE+E ++V++M GV+SVFPN K QL TT SWDFMG  +  K  R
Sbjct: 68  LVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKR 127

Query: 61  AQSVENDIIVGVIDSGIWPESPSFSD-GYGPPPAKWKGRCEVSANFSCNNKIIGARSYRS 120
             +VE+D I+GVIDSGI PES SFSD G+GPPP KWKG C    NF+CNNK+IGAR Y S
Sbjct: 128 NPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDYTS 187

Query: 121 DGQLPEGDIKGPGDSDGHGTHTASTVAGGLVRGASMVGLGLGTARGSRGPFR-------- 180
           +         G  D DGHGTHTAST AG  V  AS  G+G GT RG     R        
Sbjct: 188 E---------GTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCT 247

Query: 181 ----AHRRALSA-----------------EIWSDYYFNDYLATGSFHAINRGILSSLAVG 240
               +    LSA                 +  +  + ND +A G+FHA+ +G+L+  + G
Sbjct: 248 PTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAG 307

Query: 241 NNGPNFTTIVNFSPWSLSVAASSTDRKFVTRVELGN 245
           N+GP   ++   +PW L+VAAS+T+R FVT+V LGN
Sbjct: 308 NSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGN 334

BLAST of MC09g1435 vs. ExPASy Swiss-Prot
Match: Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 3.0e-59
Identity = 126/276 (45.65%), Postives = 168/276 (60.87%), Query Frame = 0

Query: 1   LLHSYKRSFNGFVVKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSQ--KVDR 60
           L+ SYKRSFNGF  +LTE+E   ++E+EGV+SVFPN   QLHTT SWDFMG  +     R
Sbjct: 69  LVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKR 128

Query: 61  AQSVENDIIVGVIDSGIWPESPSFSD-GYGPPPAKWKGRCEVSANFSCNNKIIGARSYRS 120
             ++E+D I+GVID+GIWPES SFSD G+GPPP KWKG C    NF+CNNK+IGAR Y S
Sbjct: 129 NLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTS 188

Query: 121 DGQLPEGDIKGPGDSDGHGTHTASTVAGGLVRGASMVGLGLGTARG-------------- 180
           +         G  D+ GHGTHTAST AG  V+  S  G+G GT RG              
Sbjct: 189 E---------GTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCT 248

Query: 181 -----SRGPFRAHRRALSAEI----------WSDYYFNDYLATGSFHAINRGILSSLAVG 240
                S     +   A++  +          +   + +D +A G+FHA+ +GIL+  + G
Sbjct: 249 DSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAG 308

Query: 241 NNGPNFTTIVNFSPWSLSVAASSTDRKFVTRVELGN 245
           N+GP  TT+ + +PW  +VAAS+T+R F+T+V LGN
Sbjct: 309 NSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGN 335

BLAST of MC09g1435 vs. NCBI nr
Match: XP_016900178.1 (PREDICTED: cucumisin-like isoform X2 [Cucumis melo])

HSP 1 Score: 372 bits (956), Expect = 2.36e-122
Identity = 194/277 (70.04%), Postives = 215/277 (77.62%), Query Frame = 0

Query: 1   LLHSYKRSFNGFVVKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSQKVDRAQ 60
           LLHS+KRSFNGFV KLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFS++V R  
Sbjct: 68  LLHSFKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVP 127

Query: 61  SVENDIIVGVIDSGIWPESPSFSD-GYGPPPAKWKGRCEVSANFSCNNKIIGARSYRSDG 120
           +VE+++IVGV+DSGIWPESPSF   GYGPPPAKWKG CEVSANFSCNNKIIGARSYRS+G
Sbjct: 128 AVESNVIVGVLDSGIWPESPSFDHTGYGPPPAKWKGSCEVSANFSCNNKIIGARSYRSNG 187

Query: 121 QLPEGDIKGPGDSDGHGTHTASTVAGGLVRGASMVGLGLGTARGSRGPFRAHRRALSAEI 180
           + PEGDIKGP DSDGHGTHTAS VAGGLVR A+M+GLGLGTARG      + R A     
Sbjct: 188 EYPEGDIKGPRDSDGHGTHTASIVAGGLVRQANMLGLGLGTARGG---VPSARIAAYKVC 247

Query: 181 WSDY--------------------------------YFNDYLATGSFHAINRGILSSLAV 240
           WSD                                 YFND +A GSFHA+ +GIL+SLAV
Sbjct: 248 WSDGCSDADILAAFDDAIADGVDIISGSLGGSGFRDYFNDSIAIGSFHAMKKGILTSLAV 307

Query: 241 GNNGPNFTTIVNFSPWSLSVAASSTDRKFVTRVELGN 244
           GNNGP+FTTIVNFSPWSLSVAAS+TDRKF T+VELG+
Sbjct: 308 GNNGPDFTTIVNFSPWSLSVAASTTDRKFETKVELGD 341

BLAST of MC09g1435 vs. NCBI nr
Match: XP_016900177.1 (PREDICTED: cucumisin-like isoform X1 [Cucumis melo] >KAA0034649.1 cucumisin-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 372 bits (956), Expect = 1.75e-121
Identity = 194/277 (70.04%), Postives = 215/277 (77.62%), Query Frame = 0

Query: 1   LLHSYKRSFNGFVVKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSQKVDRAQ 60
           LLHS+KRSFNGFV KLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFS++V R  
Sbjct: 68  LLHSFKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVP 127

Query: 61  SVENDIIVGVIDSGIWPESPSFSD-GYGPPPAKWKGRCEVSANFSCNNKIIGARSYRSDG 120
           +VE+++IVGV+DSGIWPESPSF   GYGPPPAKWKG CEVSANFSCNNKIIGARSYRS+G
Sbjct: 128 AVESNVIVGVLDSGIWPESPSFDHTGYGPPPAKWKGSCEVSANFSCNNKIIGARSYRSNG 187

Query: 121 QLPEGDIKGPGDSDGHGTHTASTVAGGLVRGASMVGLGLGTARGSRGPFRAHRRALSAEI 180
           + PEGDIKGP DSDGHGTHTAS VAGGLVR A+M+GLGLGTARG      + R A     
Sbjct: 188 EYPEGDIKGPRDSDGHGTHTASIVAGGLVRQANMLGLGLGTARGG---VPSARIAAYKVC 247

Query: 181 WSDY--------------------------------YFNDYLATGSFHAINRGILSSLAV 240
           WSD                                 YFND +A GSFHA+ +GIL+SLAV
Sbjct: 248 WSDGCSDADILAAFDDAIADGVDIISGSLGGSGFRDYFNDSIAIGSFHAMKKGILTSLAV 307

Query: 241 GNNGPNFTTIVNFSPWSLSVAASSTDRKFVTRVELGN 244
           GNNGP+FTTIVNFSPWSLSVAAS+TDRKF T+VELG+
Sbjct: 308 GNNGPDFTTIVNFSPWSLSVAASTTDRKFETKVELGD 341

BLAST of MC09g1435 vs. NCBI nr
Match: KAE8648818.1 (hypothetical protein Csa_008881 [Cucumis sativus])

HSP 1 Score: 369 bits (947), Expect = 3.80e-120
Identity = 193/277 (69.68%), Postives = 213/277 (76.90%), Query Frame = 0

Query: 1   LLHSYKRSFNGFVVKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSQKVDRAQ 60
           LLHS+KRSFNGFV KLTEAE KKVSEMEGVISVFPNGKKQLHTTRSWDFMGFS++V R  
Sbjct: 66  LLHSFKRSFNGFVAKLTEAEVKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVP 125

Query: 61  SVENDIIVGVIDSGIWPESPSFSD-GYGPPPAKWKGRCEVSANFSCNNKIIGARSYRSDG 120
           +VE+++IVGV+DSGIWPESPSF   GYG PPAKWKG CEVSANFSCNNKIIGARSYRS+G
Sbjct: 126 AVESNVIVGVLDSGIWPESPSFDHAGYGSPPAKWKGSCEVSANFSCNNKIIGARSYRSNG 185

Query: 121 QLPEGDIKGPGDSDGHGTHTASTVAGGLVRGASMVGLGLGTARGSRGPFRAHRRALSAEI 180
           + PEGDIKGP DSDGHGTHTAS VAGGLVR ASM+GLGLGTARG      + R A     
Sbjct: 186 EYPEGDIKGPRDSDGHGTHTASIVAGGLVRRASMLGLGLGTARGG---VPSARIAAYKVC 245

Query: 181 WSDY--------------------------------YFNDYLATGSFHAINRGILSSLAV 240
           WSD                                 YFND +A GSFHA+ +GIL+SLAV
Sbjct: 246 WSDGCSDADILAAFDDAIADGVDIISGSLGGSGARDYFNDSIAIGSFHAMKKGILTSLAV 305

Query: 241 GNNGPNFTTIVNFSPWSLSVAASSTDRKFVTRVELGN 244
           GNNGP+FTTIVNFSPWSLSVAAS+TDRKF T+VELG+
Sbjct: 306 GNNGPDFTTIVNFSPWSLSVAASTTDRKFETKVELGD 339

BLAST of MC09g1435 vs. NCBI nr
Match: XP_031741129.1 (cucumisin-like [Cucumis sativus])

HSP 1 Score: 369 bits (947), Expect = 4.11e-120
Identity = 193/277 (69.68%), Postives = 213/277 (76.90%), Query Frame = 0

Query: 1   LLHSYKRSFNGFVVKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSQKVDRAQ 60
           LLHS+KRSFNGFV KLTEAE KKVSEMEGVISVFPNGKKQLHTTRSWDFMGFS++V R  
Sbjct: 69  LLHSFKRSFNGFVAKLTEAEVKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVP 128

Query: 61  SVENDIIVGVIDSGIWPESPSFSD-GYGPPPAKWKGRCEVSANFSCNNKIIGARSYRSDG 120
           +VE+++IVGV+DSGIWPESPSF   GYG PPAKWKG CEVSANFSCNNKIIGARSYRS+G
Sbjct: 129 AVESNVIVGVLDSGIWPESPSFDHAGYGSPPAKWKGSCEVSANFSCNNKIIGARSYRSNG 188

Query: 121 QLPEGDIKGPGDSDGHGTHTASTVAGGLVRGASMVGLGLGTARGSRGPFRAHRRALSAEI 180
           + PEGDIKGP DSDGHGTHTAS VAGGLVR ASM+GLGLGTARG      + R A     
Sbjct: 189 EYPEGDIKGPRDSDGHGTHTASIVAGGLVRRASMLGLGLGTARGG---VPSARIAAYKVC 248

Query: 181 WSDY--------------------------------YFNDYLATGSFHAINRGILSSLAV 240
           WSD                                 YFND +A GSFHA+ +GIL+SLAV
Sbjct: 249 WSDGCSDADILAAFDDAIADGVDIISGSLGGSGARDYFNDSIAIGSFHAMKKGILTSLAV 308

Query: 241 GNNGPNFTTIVNFSPWSLSVAASSTDRKFVTRVELGN 244
           GNNGP+FTTIVNFSPWSLSVAAS+TDRKF T+VELG+
Sbjct: 309 GNNGPDFTTIVNFSPWSLSVAASTTDRKFETKVELGD 342

BLAST of MC09g1435 vs. NCBI nr
Match: XP_022957157.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 367 bits (942), Expect = 2.19e-119
Identity = 193/277 (69.68%), Postives = 214/277 (77.26%), Query Frame = 0

Query: 1   LLHSYKRSFNGFVVKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSQKVDRAQ 60
           L+HSYKRSFNGFV KLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFS++V R  
Sbjct: 67  LIHSYKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVP 126

Query: 61  SVENDIIVGVIDSGIWPESPSFSD-GYGPPPAKWKGRCEVSANFSCNNKIIGARSYRSDG 120
           +VE++IIVGV DSGIWPESPSF D GYGPPPAKW+GRCEVSANFSCN+KIIGARSYRS+G
Sbjct: 127 AVESNIIVGVFDSGIWPESPSFDDAGYGPPPAKWRGRCEVSANFSCNDKIIGARSYRSNG 186

Query: 121 QLPEGDIKGPGDSDGHGTHTASTVAGGLVRGASMVGLGLGTARGSRGPFRAHRRALSAEI 180
           +  E DIKGP DSDGHGTHTASTVAGGLVR ASM+GLGLGTARG      + R A     
Sbjct: 187 KHLENDIKGPIDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGG---VPSARIAAYKVC 246

Query: 181 WSDY--------------------------------YFNDYLATGSFHAINRGILSSLAV 240
           WSD                                 YFND +A GSFHA+ +GIL++LAV
Sbjct: 247 WSDGCTDADILAAFDDAIADGVDIISGSLGGSSVKDYFNDSIAIGSFHAMQKGILTTLAV 306

Query: 241 GNNGPNFTTIVNFSPWSLSVAASSTDRKFVTRVELGN 244
           GNNGP FTTIVNFSPWSLSVAAS+TDRKF T+V+LG+
Sbjct: 307 GNNGPEFTTIVNFSPWSLSVAASTTDRKFETKVKLGD 340

BLAST of MC09g1435 vs. ExPASy TrEMBL
Match: A0A1S4DW35 (cucumisin-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489381 PE=3 SV=1)

HSP 1 Score: 372 bits (956), Expect = 1.14e-122
Identity = 194/277 (70.04%), Postives = 215/277 (77.62%), Query Frame = 0

Query: 1   LLHSYKRSFNGFVVKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSQKVDRAQ 60
           LLHS+KRSFNGFV KLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFS++V R  
Sbjct: 68  LLHSFKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVP 127

Query: 61  SVENDIIVGVIDSGIWPESPSFSD-GYGPPPAKWKGRCEVSANFSCNNKIIGARSYRSDG 120
           +VE+++IVGV+DSGIWPESPSF   GYGPPPAKWKG CEVSANFSCNNKIIGARSYRS+G
Sbjct: 128 AVESNVIVGVLDSGIWPESPSFDHTGYGPPPAKWKGSCEVSANFSCNNKIIGARSYRSNG 187

Query: 121 QLPEGDIKGPGDSDGHGTHTASTVAGGLVRGASMVGLGLGTARGSRGPFRAHRRALSAEI 180
           + PEGDIKGP DSDGHGTHTAS VAGGLVR A+M+GLGLGTARG      + R A     
Sbjct: 188 EYPEGDIKGPRDSDGHGTHTASIVAGGLVRQANMLGLGLGTARGG---VPSARIAAYKVC 247

Query: 181 WSDY--------------------------------YFNDYLATGSFHAINRGILSSLAV 240
           WSD                                 YFND +A GSFHA+ +GIL+SLAV
Sbjct: 248 WSDGCSDADILAAFDDAIADGVDIISGSLGGSGFRDYFNDSIAIGSFHAMKKGILTSLAV 307

Query: 241 GNNGPNFTTIVNFSPWSLSVAASSTDRKFVTRVELGN 244
           GNNGP+FTTIVNFSPWSLSVAAS+TDRKF T+VELG+
Sbjct: 308 GNNGPDFTTIVNFSPWSLSVAASTTDRKFETKVELGD 341

BLAST of MC09g1435 vs. ExPASy TrEMBL
Match: A0A5A7SZX9 (Cucumisin-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G006680 PE=3 SV=1)

HSP 1 Score: 372 bits (956), Expect = 8.49e-122
Identity = 194/277 (70.04%), Postives = 215/277 (77.62%), Query Frame = 0

Query: 1   LLHSYKRSFNGFVVKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSQKVDRAQ 60
           LLHS+KRSFNGFV KLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFS++V R  
Sbjct: 68  LLHSFKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVP 127

Query: 61  SVENDIIVGVIDSGIWPESPSFSD-GYGPPPAKWKGRCEVSANFSCNNKIIGARSYRSDG 120
           +VE+++IVGV+DSGIWPESPSF   GYGPPPAKWKG CEVSANFSCNNKIIGARSYRS+G
Sbjct: 128 AVESNVIVGVLDSGIWPESPSFDHTGYGPPPAKWKGSCEVSANFSCNNKIIGARSYRSNG 187

Query: 121 QLPEGDIKGPGDSDGHGTHTASTVAGGLVRGASMVGLGLGTARGSRGPFRAHRRALSAEI 180
           + PEGDIKGP DSDGHGTHTAS VAGGLVR A+M+GLGLGTARG      + R A     
Sbjct: 188 EYPEGDIKGPRDSDGHGTHTASIVAGGLVRQANMLGLGLGTARGG---VPSARIAAYKVC 247

Query: 181 WSDY--------------------------------YFNDYLATGSFHAINRGILSSLAV 240
           WSD                                 YFND +A GSFHA+ +GIL+SLAV
Sbjct: 248 WSDGCSDADILAAFDDAIADGVDIISGSLGGSGFRDYFNDSIAIGSFHAMKKGILTSLAV 307

Query: 241 GNNGPNFTTIVNFSPWSLSVAASSTDRKFVTRVELGN 244
           GNNGP+FTTIVNFSPWSLSVAAS+TDRKF T+VELG+
Sbjct: 308 GNNGPDFTTIVNFSPWSLSVAASTTDRKFETKVELGD 341

BLAST of MC09g1435 vs. ExPASy TrEMBL
Match: A0A1S4DW16 (cucumisin-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489381 PE=3 SV=1)

HSP 1 Score: 372 bits (956), Expect = 8.49e-122
Identity = 194/277 (70.04%), Postives = 215/277 (77.62%), Query Frame = 0

Query: 1   LLHSYKRSFNGFVVKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSQKVDRAQ 60
           LLHS+KRSFNGFV KLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFS++V R  
Sbjct: 68  LLHSFKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVP 127

Query: 61  SVENDIIVGVIDSGIWPESPSFSD-GYGPPPAKWKGRCEVSANFSCNNKIIGARSYRSDG 120
           +VE+++IVGV+DSGIWPESPSF   GYGPPPAKWKG CEVSANFSCNNKIIGARSYRS+G
Sbjct: 128 AVESNVIVGVLDSGIWPESPSFDHTGYGPPPAKWKGSCEVSANFSCNNKIIGARSYRSNG 187

Query: 121 QLPEGDIKGPGDSDGHGTHTASTVAGGLVRGASMVGLGLGTARGSRGPFRAHRRALSAEI 180
           + PEGDIKGP DSDGHGTHTAS VAGGLVR A+M+GLGLGTARG      + R A     
Sbjct: 188 EYPEGDIKGPRDSDGHGTHTASIVAGGLVRQANMLGLGLGTARGG---VPSARIAAYKVC 247

Query: 181 WSDY--------------------------------YFNDYLATGSFHAINRGILSSLAV 240
           WSD                                 YFND +A GSFHA+ +GIL+SLAV
Sbjct: 248 WSDGCSDADILAAFDDAIADGVDIISGSLGGSGFRDYFNDSIAIGSFHAMKKGILTSLAV 307

Query: 241 GNNGPNFTTIVNFSPWSLSVAASSTDRKFVTRVELGN 244
           GNNGP+FTTIVNFSPWSLSVAAS+TDRKF T+VELG+
Sbjct: 308 GNNGPDFTTIVNFSPWSLSVAASTTDRKFETKVELGD 341

BLAST of MC09g1435 vs. ExPASy TrEMBL
Match: A0A0A0KWL9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G613590 PE=3 SV=1)

HSP 1 Score: 369 bits (947), Expect = 1.99e-120
Identity = 193/277 (69.68%), Postives = 213/277 (76.90%), Query Frame = 0

Query: 1   LLHSYKRSFNGFVVKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSQKVDRAQ 60
           LLHS+KRSFNGFV KLTEAE KKVSEMEGVISVFPNGKKQLHTTRSWDFMGFS++V R  
Sbjct: 69  LLHSFKRSFNGFVAKLTEAEVKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVP 128

Query: 61  SVENDIIVGVIDSGIWPESPSFSD-GYGPPPAKWKGRCEVSANFSCNNKIIGARSYRSDG 120
           +VE+++IVGV+DSGIWPESPSF   GYG PPAKWKG CEVSANFSCNNKIIGARSYRS+G
Sbjct: 129 AVESNVIVGVLDSGIWPESPSFDHAGYGSPPAKWKGSCEVSANFSCNNKIIGARSYRSNG 188

Query: 121 QLPEGDIKGPGDSDGHGTHTASTVAGGLVRGASMVGLGLGTARGSRGPFRAHRRALSAEI 180
           + PEGDIKGP DSDGHGTHTAS VAGGLVR ASM+GLGLGTARG      + R A     
Sbjct: 189 EYPEGDIKGPRDSDGHGTHTASIVAGGLVRRASMLGLGLGTARGG---VPSARIAAYKVC 248

Query: 181 WSDY--------------------------------YFNDYLATGSFHAINRGILSSLAV 240
           WSD                                 YFND +A GSFHA+ +GIL+SLAV
Sbjct: 249 WSDGCSDADILAAFDDAIADGVDIISGSLGGSGARDYFNDSIAIGSFHAMKKGILTSLAV 308

Query: 241 GNNGPNFTTIVNFSPWSLSVAASSTDRKFVTRVELGN 244
           GNNGP+FTTIVNFSPWSLSVAAS+TDRKF T+VELG+
Sbjct: 309 GNNGPDFTTIVNFSPWSLSVAASTTDRKFETKVELGD 342

BLAST of MC09g1435 vs. ExPASy TrEMBL
Match: A0A6J1GYD4 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458626 PE=3 SV=1)

HSP 1 Score: 367 bits (942), Expect = 1.06e-119
Identity = 193/277 (69.68%), Postives = 214/277 (77.26%), Query Frame = 0

Query: 1   LLHSYKRSFNGFVVKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSQKVDRAQ 60
           L+HSYKRSFNGFV KLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFS++V R  
Sbjct: 67  LIHSYKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVP 126

Query: 61  SVENDIIVGVIDSGIWPESPSFSD-GYGPPPAKWKGRCEVSANFSCNNKIIGARSYRSDG 120
           +VE++IIVGV DSGIWPESPSF D GYGPPPAKW+GRCEVSANFSCN+KIIGARSYRS+G
Sbjct: 127 AVESNIIVGVFDSGIWPESPSFDDAGYGPPPAKWRGRCEVSANFSCNDKIIGARSYRSNG 186

Query: 121 QLPEGDIKGPGDSDGHGTHTASTVAGGLVRGASMVGLGLGTARGSRGPFRAHRRALSAEI 180
           +  E DIKGP DSDGHGTHTASTVAGGLVR ASM+GLGLGTARG      + R A     
Sbjct: 187 KHLENDIKGPIDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGG---VPSARIAAYKVC 246

Query: 181 WSDY--------------------------------YFNDYLATGSFHAINRGILSSLAV 240
           WSD                                 YFND +A GSFHA+ +GIL++LAV
Sbjct: 247 WSDGCTDADILAAFDDAIADGVDIISGSLGGSSVKDYFNDSIAIGSFHAMQKGILTTLAV 306

Query: 241 GNNGPNFTTIVNFSPWSLSVAASSTDRKFVTRVELGN 244
           GNNGP FTTIVNFSPWSLSVAAS+TDRKF T+V+LG+
Sbjct: 307 GNNGPEFTTIVNFSPWSLSVAASTTDRKFETKVKLGD 340

BLAST of MC09g1435 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 235.3 bits (599), Expect = 5.0e-62
Identity = 130/273 (47.62%), Postives = 167/273 (61.17%), Query Frame = 0

Query: 1   LLHSYKRSFNGFVVKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSQKVDRAQ 60
           L+ SYKRSFNGF   L++AE++K+  M+ V+SVFP+   +L TTRSWDF+GF +K  R  
Sbjct: 32  LVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRES 91

Query: 61  SVENDIIVGVIDSGIWPESPSFSD-GYGPPPAKWKGRCEVSANFSCNNKIIGARSYRSDG 120
             E+D+IVGVIDSGIWPES SF D G+GPPP KWKG C+    F+CNNK+IGAR Y    
Sbjct: 92  VKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIGARFYNKFA 151

Query: 121 QLPEGDIKGPGDSDGHGTHTASTVAGGLVRGASMVGLGLGTARGSRGPFR--AHR----- 180
                      D +GHGTHTAST AG  V+ AS  GL  GTARG     R  A++     
Sbjct: 152 D-------SARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCFNR 211

Query: 181 ------------------RALSAEIWSDY---YFNDYLATGSFHAINRGILSSLAVGNNG 240
                               +S  I +DY     N  +A GSFHA+ RGI+++ + GNNG
Sbjct: 212 CNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNG 271

Query: 241 PNFTTIVNFSPWSLSVAASSTDRKFVTRVELGN 245
           P+  ++ N SPW ++VAAS TDR+F+ RV LGN
Sbjct: 272 PDQGSVANVSPWMITVAASGTDRQFIDRVVLGN 297

BLAST of MC09g1435 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 230.3 bits (586), Expect = 1.6e-60
Identity = 132/276 (47.83%), Postives = 168/276 (60.87%), Query Frame = 0

Query: 1   LLHSYKRSFNGFVVKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSQ--KVDR 60
           L+ SYKRSFNGF  +LTE+E ++V++M GV+SVFPN K QL TT SWDFMG  +  K  R
Sbjct: 68  LVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKR 127

Query: 61  AQSVENDIIVGVIDSGIWPESPSFSD-GYGPPPAKWKGRCEVSANFSCNNKIIGARSYRS 120
             +VE+D I+GVIDSGI PES SFSD G+GPPP KWKG C    NF+CNNK+IGAR Y S
Sbjct: 128 NPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDYTS 187

Query: 121 DGQLPEGDIKGPGDSDGHGTHTASTVAGGLVRGASMVGLGLGTARGSRGPFR-------- 180
           +         G  D DGHGTHTAST AG  V  AS  G+G GT RG     R        
Sbjct: 188 E---------GTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCT 247

Query: 181 ----AHRRALSA-----------------EIWSDYYFNDYLATGSFHAINRGILSSLAVG 240
               +    LSA                 +  +  + ND +A G+FHA+ +G+L+  + G
Sbjct: 248 PTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAG 307

Query: 241 NNGPNFTTIVNFSPWSLSVAASSTDRKFVTRVELGN 245
           N+GP   ++   +PW L+VAAS+T+R FVT+V LGN
Sbjct: 308 NSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGN 334

BLAST of MC09g1435 vs. TAIR 10
Match: AT5G59090.1 (subtilase 4.12 )

HSP 1 Score: 229.9 bits (585), Expect = 2.1e-60
Identity = 126/276 (45.65%), Postives = 168/276 (60.87%), Query Frame = 0

Query: 1   LLHSYKRSFNGFVVKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSQ--KVDR 60
           L+ SYKRSFNGF  +LTE+E   ++E+EGV+SVFPN   QLHTT SWDFMG  +     R
Sbjct: 69  LVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKR 128

Query: 61  AQSVENDIIVGVIDSGIWPESPSFSD-GYGPPPAKWKGRCEVSANFSCNNKIIGARSYRS 120
             ++E+D I+GVID+GIWPES SFSD G+GPPP KWKG C    NF+CNNK+IGAR Y S
Sbjct: 129 NLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTS 188

Query: 121 DGQLPEGDIKGPGDSDGHGTHTASTVAGGLVRGASMVGLGLGTARG-------------- 180
           +         G  D+ GHGTHTAST AG  V+  S  G+G GT RG              
Sbjct: 189 E---------GTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCT 248

Query: 181 -----SRGPFRAHRRALSAEI----------WSDYYFNDYLATGSFHAINRGILSSLAVG 240
                S     +   A++  +          +   + +D +A G+FHA+ +GIL+  + G
Sbjct: 249 DSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAG 308

Query: 241 NNGPNFTTIVNFSPWSLSVAASSTDRKFVTRVELGN 245
           N+GP  TT+ + +PW  +VAAS+T+R F+T+V LGN
Sbjct: 309 NSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGN 335

BLAST of MC09g1435 vs. TAIR 10
Match: AT5G59090.2 (subtilase 4.12 )

HSP 1 Score: 229.9 bits (585), Expect = 2.1e-60
Identity = 126/276 (45.65%), Postives = 168/276 (60.87%), Query Frame = 0

Query: 1   LLHSYKRSFNGFVVKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSQ--KVDR 60
           L+ SYKRSFNGF  +LTE+E   ++E+EGV+SVFPN   QLHTT SWDFMG  +     R
Sbjct: 69  LVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKR 128

Query: 61  AQSVENDIIVGVIDSGIWPESPSFSD-GYGPPPAKWKGRCEVSANFSCNNKIIGARSYRS 120
             ++E+D I+GVID+GIWPES SFSD G+GPPP KWKG C    NF+CNNK+IGAR Y S
Sbjct: 129 NLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTS 188

Query: 121 DGQLPEGDIKGPGDSDGHGTHTASTVAGGLVRGASMVGLGLGTARG-------------- 180
           +         G  D+ GHGTHTAST AG  V+  S  G+G GT RG              
Sbjct: 189 E---------GTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCT 248

Query: 181 -----SRGPFRAHRRALSAEI----------WSDYYFNDYLATGSFHAINRGILSSLAVG 240
                S     +   A++  +          +   + +D +A G+FHA+ +GIL+  + G
Sbjct: 249 DSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAG 308

Query: 241 NNGPNFTTIVNFSPWSLSVAASSTDRKFVTRVELGN 245
           N+GP  TT+ + +PW  +VAAS+T+R F+T+V LGN
Sbjct: 309 NSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGN 335

BLAST of MC09g1435 vs. TAIR 10
Match: AT5G58840.1 (Subtilase family protein )

HSP 1 Score: 229.2 bits (583), Expect = 3.6e-60
Identity = 127/276 (46.01%), Postives = 168/276 (60.87%), Query Frame = 0

Query: 1   LLHSYKRSFNGFVVKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSQ--KVDR 60
           L+ SYKRSFNGF  +LTE+E ++V+EMEGV+SVFP+   +L TT SWDF+G  +     R
Sbjct: 72  LVRSYKRSFNGFAARLTESERERVAEMEGVVSVFPDINYKLQTTASWDFLGLKEGKNTKR 131

Query: 61  AQSVENDIIVGVIDSGIWPESPSFSD-GYGPPPAKWKGRCEVSANFSCNNKIIGARSYRS 120
             ++E+D I+G IDSGIWPES SFSD G+GPPP KWKG C    NF+CNNK+IGAR Y +
Sbjct: 132 NLAIESDTIIGFIDSGIWPESESFSDKGFGPPPKKWKGVCSAGKNFTCNNKLIGARDYTN 191

Query: 121 DGQLPEGDIKGPGDSDGHGTHTASTVAGGLVRGASMVGLGLGTARGSRGPFRAHRRALSA 180
           +         G  D +GHGTHTAST AG  V+  S  G+G GTARG     R       +
Sbjct: 192 E---------GTRDIEGHGTHTASTAAGNAVKNTSFYGIGNGTARGGVPASRIAAYKACS 251

Query: 181 EI----------WSD-------------------YYFNDYLATGSFHAINRGILSSLAVG 240
           E+          + D                    Y  D +A G+FHA+ +GIL+  + G
Sbjct: 252 EMGCTTESVLSAFDDAIADGVDLISISLGANLVRTYETDPIAIGAFHAMVKGILTVQSAG 311

Query: 241 NNGPNFTTIVNFSPWSLSVAASSTDRKFVTRVELGN 245
           N GPN  ++++ +PW L+VAAS+T+R FVT+V LGN
Sbjct: 312 NGGPNPGSVMSVAPWILTVAASNTNRGFVTKVVLGN 338