MC09g1222 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g1222
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionMembrane-associated kinase regulator 4
LocationMC09: 18255625 .. 18256674 (-)
RNA-Seq ExpressionMC09g1222
SyntenyMC09g1222
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGTTGACATCCCATCTTATGATCCAGCAGATGATGACTATATAGACATGGAAGTTAGTTCATACACCAACTTTCTATGCCACTCCAAAAGCTCTCCGCCGCGTCCCAGAGAGTTCGAGTTTCAAATGTCTTTGAATTCTCGGGGGAGAGAGCCTACAACTTCTCCGGCCGATGAGCTCTTCTACAATGGAAAGCTTCTTCCCCTTCACCATCCCCCACGCCTGCAAATGGTAGAGAAACTCTTGCAGAAGTCTAATTCTGATTATGATTGCAGAAAAGACAATTTTGAAGAATTTTACAGCACTCCATTAACCACTGCTGTCAATACACCTACTGCAACAAGTACCCCCTTTGAATCCTGCAACATTTCTCCTTCTGAATCTTGTCGGATTAGTAGGGAACTAAATCCAGATGAATACCTTCTAGAGTATTCAGCAGATGCAACTTATTTGAATGGTGAAAGTTCGAAAAAGTCTTGGACCAGAAAGCTTAAGCATTCTTCCCTTGGTCTCAAGCTGAAAGCTTCCAGGGCTTACATCAAATCTTTGTTTACAAAATCAGGCTGCTCAGATGAGTCCTGTGCAGCAGCCACAAAGATTGCAGATGAAGGATCAGCTTCAAAAGCCCACGAATGTTCAAAGAAGTGTGCTAAAGTGGAAAAGAGAAAGCCACTTGGACAAATTCCAAGCTCTATTGCCAATTTCAACAAGGAAAAAGGCAATACGGAAAACCGTGATCGCAACAGAAGATCATTCTCAATGACCATTAAGAGACAACCAGATATAAAACCATCATCTTCTGCATCATTACATACCTCTTCCTCTTCATATTCACACAATTCAAGCGCGTATTTTCTGAAGAGATGTGGCAGCGTGAATTCAGAGATCGAGAATTCAATTCAGGGAGCAATTGCACACTGCAAGCAGTCTCACTCTCAGCAACCATTCCGTTCAAGGAAGACAGTTAGTGAAGTTGGGATTTACTTGTTATCATCTTCCAGCCTTGCAGTTTCTGGTGATCAGGAAATACCAGAACTTTGCAGGGGC

mRNA sequence

ATGGCTGTTGACATCCCATCTTATGATCCAGCAGATGATGACTATATAGACATGGAAGTTAGTTCATACACCAACTTTCTATGCCACTCCAAAAGCTCTCCGCCGCGTCCCAGAGAGTTCGAGTTTCAAATGTCTTTGAATTCTCGGGGGAGAGAGCCTACAACTTCTCCGGCCGATGAGCTCTTCTACAATGGAAAGCTTCTTCCCCTTCACCATCCCCCACGCCTGCAAATGGTAGAGAAACTCTTGCAGAAGTCTAATTCTGATTATGATTGCAGAAAAGACAATTTTGAAGAATTTTACAGCACTCCATTAACCACTGCTGTCAATACACCTACTGCAACAAGTACCCCCTTTGAATCCTGCAACATTTCTCCTTCTGAATCTTGTCGGATTAGTAGGGAACTAAATCCAGATGAATACCTTCTAGAGTATTCAGCAGATGCAACTTATTTGAATGGTGAAAGTTCGAAAAAGTCTTGGACCAGAAAGCTTAAGCATTCTTCCCTTGGTCTCAAGCTGAAAGCTTCCAGGGCTTACATCAAATCTTTGTTTACAAAATCAGGCTGCTCAGATGAGTCCTGTGCAGCAGCCACAAAGATTGCAGATGAAGGATCAGCTTCAAAAGCCCACGAATGTTCAAAGAAGTGTGCTAAAGTGGAAAAGAGAAAGCCACTTGGACAAATTCCAAGCTCTATTGCCAATTTCAACAAGGAAAAAGGCAATACGGAAAACCGTGATCGCAACAGAAGATCATTCTCAATGACCATTAAGAGACAACCAGATATAAAACCATCATCTTCTGCATCATTACATACCTCTTCCTCTTCATATTCACACAATTCAAGCGCGTATTTTCTGAAGAGATGTGGCAGCGTGAATTCAGAGATCGAGAATTCAATTCAGGGAGCAATTGCACACTGCAAGCAGTCTCACTCTCAGCAACCATTCCGTTCAAGGAAGACAGTTAGTGAAGTTGGGATTTACTTGTTATCATCTTCCAGCCTTGCAGTTTCTGGTGATCAGGAAATACCAGAACTTTGCAGGGGC

Coding sequence (CDS)

ATGGCTGTTGACATCCCATCTTATGATCCAGCAGATGATGACTATATAGACATGGAAGTTAGTTCATACACCAACTTTCTATGCCACTCCAAAAGCTCTCCGCCGCGTCCCAGAGAGTTCGAGTTTCAAATGTCTTTGAATTCTCGGGGGAGAGAGCCTACAACTTCTCCGGCCGATGAGCTCTTCTACAATGGAAAGCTTCTTCCCCTTCACCATCCCCCACGCCTGCAAATGGTAGAGAAACTCTTGCAGAAGTCTAATTCTGATTATGATTGCAGAAAAGACAATTTTGAAGAATTTTACAGCACTCCATTAACCACTGCTGTCAATACACCTACTGCAACAAGTACCCCCTTTGAATCCTGCAACATTTCTCCTTCTGAATCTTGTCGGATTAGTAGGGAACTAAATCCAGATGAATACCTTCTAGAGTATTCAGCAGATGCAACTTATTTGAATGGTGAAAGTTCGAAAAAGTCTTGGACCAGAAAGCTTAAGCATTCTTCCCTTGGTCTCAAGCTGAAAGCTTCCAGGGCTTACATCAAATCTTTGTTTACAAAATCAGGCTGCTCAGATGAGTCCTGTGCAGCAGCCACAAAGATTGCAGATGAAGGATCAGCTTCAAAAGCCCACGAATGTTCAAAGAAGTGTGCTAAAGTGGAAAAGAGAAAGCCACTTGGACAAATTCCAAGCTCTATTGCCAATTTCAACAAGGAAAAAGGCAATACGGAAAACCGTGATCGCAACAGAAGATCATTCTCAATGACCATTAAGAGACAACCAGATATAAAACCATCATCTTCTGCATCATTACATACCTCTTCCTCTTCATATTCACACAATTCAAGCGCGTATTTTCTGAAGAGATGTGGCAGCGTGAATTCAGAGATCGAGAATTCAATTCAGGGAGCAATTGCACACTGCAAGCAGTCTCACTCTCAGCAACCATTCCGTTCAAGGAAGACAGTTAGTGAAGTTGGGATTTACTTGTTATCATCTTCCAGCCTTGCAGTTTCTGGTGATCAGGAAATACCAGAACTTTGCAGGGGC

Protein sequence

MAVDIPSYDPADDDYIDMEVSSYTNFLCHSKSSPPRPREFEFQMSLNSRGREPTTSPADELFYNGKLLPLHHPPRLQMVEKLLQKSNSDYDCRKDNFEEFYSTPLTTAVNTPTATSTPFESCNISPSESCRISRELNPDEYLLEYSADATYLNGESSKKSWTRKLKHSSLGLKLKASRAYIKSLFTKSGCSDESCAAATKIADEGSASKAHECSKKCAKVEKRKPLGQIPSSIANFNKEKGNTENRDRNRRSFSMTIKRQPDIKPSSSASLHTSSSSYSHNSSAYFLKRCGSVNSEIENSIQGAIAHCKQSHSQQPFRSRKTVSEVGIYLLSSSSLAVSGDQEIPELCRG
Homology
BLAST of MC09g1222 vs. ExPASy Swiss-Prot
Match: O80624 (Probable membrane-associated kinase regulator 4 OS=Arabidopsis thaliana OX=3702 GN=MAKR4 PE=3 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 8.1e-66
Identity = 173/358 (48.32%), Postives = 222/358 (62.01%), Query Frame = 0

Query: 1   MAVDIPSYDPADDDYIDMEVSSYTNFLCHSKSSPPRPREFEFQMS-LNSRGREPTTSPAD 60
           MA  +   D  ++DYIDMEV+S+TN L     S   PREFEFQMS L     + TTSPAD
Sbjct: 1   MAAYLERCDSVEEDYIDMEVTSFTN-LVRKTLSNNYPREFEFQMSHLCPLEIDKTTSPAD 60

Query: 61  ELFYNGKLLPLHHPPRLQMVEKLLQKSNSDYDCRKDNFEEFYSTPLTT-AVNTPTATSTP 120
           ELFY GKLLPLH PPRLQMV+K+L+    D        +EFYSTPL T  V TP  ++TP
Sbjct: 61  ELFYKGKLLPLHLPPRLQMVQKILEDYTFD--------DEFYSTPLATGTVTTPVTSNTP 120

Query: 121 FESCNISPSESCRISRELNPDEYLLEYSADATYLNGESSKKSWTRKL---KHSSLGLKLK 180
           FESC +SP+ESC++S+ELNP++Y LEYS        +  KKSWT KL   K SSLG K+K
Sbjct: 121 FESCTVSPAESCQVSKELNPEDYFLEYSDSLE--EDDEKKKSWTTKLRLMKQSSLGTKIK 180

Query: 181 ASRAYIKSLFTKSGCSDE-SCA-AATKIADEGSASKAHECSKKCAKVEKRKPLGQIPSSI 240
           ASRAY++S F K+ CSDE SCA +A ++ADE S            +  + KP GQI +  
Sbjct: 181 ASRAYLRSFFGKTSCSDESSCASSAARVADEDSV----------LRYSRVKPFGQIKTER 240

Query: 241 ANFNKEKGNTENRDRNRRSFSMTIKRQPDIKPSSSASLHTSSSSYSHNSSAYFLKRCGSV 300
               K++ N      +RRSFS++++RQ     ++ +S + SS+S        FLKR  S 
Sbjct: 241 P---KKQSNGSVSGSHRRSFSVSMRRQ-----AAKSSNNKSSNSLGFR-PLQFLKRSTSS 300

Query: 301 NSEIENSIQGAIAHCKQSHSQ-QPFRSRKTVSEVGIYLLSSSSLAVSGDQEIPELCRG 351
           +SEIENSIQGAI HCKQS  Q Q  +   TV+EVG   LS+S +A   DQE  ++ RG
Sbjct: 301 SSEIENSIQGAILHCKQSQQQKQKQKQYSTVNEVGFCSLSASRIAARDDQEWAQMFRG 328

BLAST of MC09g1222 vs. ExPASy Swiss-Prot
Match: Q9ZUS8 (Probable membrane-associated kinase regulator 3 OS=Arabidopsis thaliana OX=3702 GN=MAKR3 PE=2 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 2.0e-32
Identity = 135/352 (38.35%), Postives = 180/352 (51.14%), Query Frame = 0

Query: 12  DDDYIDMEV----------SSYTNFLCHSKSSPPRPREFEFQMSLNS-RGREPTTSPADE 71
           DD YIDMEV          SS + F     SSPP+ REFEFQM  ++    E TTSPADE
Sbjct: 18  DDGYIDMEVNLSSSSSSSTSSSSFFSFPVTSSPPQSREFEFQMCSSAVASGESTTSPADE 77

Query: 72  LFYNGKLLPLHHPPRLQMVEKLLQKSNSDYDCRKDNFEEFYSTPLTTAVNTPTATSTPFE 131
           LFY G+LLPLH PPRL+MV+KLL  S+S     +        TP++        +   F 
Sbjct: 78  LFYKGQLLPLHLPPRLKMVQKLLLASSSSTAATE--------TPISPRAAADVLSPRRFS 137

Query: 132 SCNISPSESC--RISRELNPDEYLLEYSADATYLNGESSKKSWTRKLKHSSLGLKLKASR 191
           SC I   E+C   IS EL   +  +E        N      SW++K+KHSS+  KLKASR
Sbjct: 138 SCEIGQDENCFFEISTEL---KRFIE-------SNENHLGNSWSKKIKHSSITQKLKASR 197

Query: 192 AYIKSLFTKSGCSDESCAAATKIADEGSASKAHECSKKCAKVEKRKPLGQIPSSIANFNK 251
           AYIK+LF+K  CSD S                 E + +  K+E        PS ++    
Sbjct: 198 AYIKALFSKQACSDSS-----------------EINPR-FKIE--------PSKVSRKKN 257

Query: 252 EKGNTENRDR-NRRSFSMTIKRQPDIKPSSSASLHTSSSSYSHNSSAYFLKRCGSVNSEI 311
              N+EN    +RRSFS  I+R    K S+S+S   SSSS S  SS++     GS++ + 
Sbjct: 258 PFVNSENPLLIHRRSFSGVIQRHSQAKCSTSSS---SSSSASSLSSSFSFGSNGSLDLQT 317

Query: 312 --------ENSIQGAIAHCKQSHSQQPFRSRKTVSEVGIYLLSSSSLAVSGD 342
                   +NSI+GAI HCKQS + +  +S  T SE+     S +S++  GD
Sbjct: 318 LMRSSNASDNSIEGAIEHCKQSFTTR--KSNVTESEL---CSSRTSVSTCGD 317

BLAST of MC09g1222 vs. ExPASy Swiss-Prot
Match: Q3E936 (Probable membrane-associated kinase regulator 1 OS=Arabidopsis thaliana OX=3702 GN=MAKR1 PE=1 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 3.9e-04
Identity = 89/346 (25.72%), Postives = 134/346 (38.73%), Query Frame = 0

Query: 31  KSSPPRPR---------------EFEFQMSLNSRGREPTTSPADELFYNGKLLPLHHPPR 90
           +  PPRPR               EFEF +S++ R    +  PADELFY G+LLPL   PR
Sbjct: 2   RRQPPRPRNSPPQSHSSPSSSSSEFEFNISISPRKASSSLCPADELFYKGQLLPLQLSPR 61

Query: 91  LQMVEKL-LQKSNSDYDCRKDNFEEFYSTPLTTAVNTPTATSTPFESCNISPSESCRISR 150
           L +V  L    S+SDY     +     +   +T  N+ T ++  F   +  P + C  SR
Sbjct: 62  LSLVRTLGSSTSSSDYTSSSSSSVATSAARDSTESNSSTDSTASFPLLHPPPLDCCDSSR 121

Query: 151 ELNPDEYLLEYSADATYLNGESSKKSWTRKLKHSSLGLKL-KASRAYIKSLFTKSGCSDE 210
             +  +       D  +       KS       SS G  L + S  + K   T       
Sbjct: 122 PSSVTD-------DEDFFFKPPKNKS-------SSGGFSLSRFSSVFKKDPKTNLHHHSS 181

Query: 211 SCAAATKIADEGSASKAHECSKKCAK--VEKRKPLGQ--IPSSIANFNKEKGNT-ENRDR 270
           S + AT  A   S  +    +K+  +  ++K KPL +   P   +N   E  ++ ++   
Sbjct: 182 SSSTATTAAAPSSVKRMSSTAKEVIRKYMKKVKPLYEKLSPKQSSNIKTESSSSLKDSGN 241

Query: 271 NRRSFS--MTIKRQPDI-------------------KPSSSASLHTSSSSYSHNSSAYFL 316
           N R  +   T+   P +                   K    A+   SS   S N S    
Sbjct: 242 NIRGTTTVTTVTAAPTVVSSGGGLSISFSGNLMKYTKRGRCAASCPSSMRSSPNHSGVLT 301

BLAST of MC09g1222 vs. NCBI nr
Match: XP_022139254.1 (probable membrane-associated kinase regulator 4 [Momordica charantia])

HSP 1 Score: 682 bits (1759), Expect = 5.11e-247
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0

Query: 1   MAVDIPSYDPADDDYIDMEVSSYTNFLCHSKSSPPRPREFEFQMSLNSRGREPTTSPADE 60
           MAVDIPSYDPADDDYIDMEVSSYTNFLCHSKSSPPRPREFEFQMSLNSRGREPTTSPADE
Sbjct: 1   MAVDIPSYDPADDDYIDMEVSSYTNFLCHSKSSPPRPREFEFQMSLNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVEKLLQKSNSDYDCRKDNFEEFYSTPLTTAVNTPTATSTPFE 120
           LFYNGKLLPLHHPPRLQMVEKLLQKSNSDYDCRKDNFEEFYSTPLTTAVNTPTATSTPFE
Sbjct: 61  LFYNGKLLPLHHPPRLQMVEKLLQKSNSDYDCRKDNFEEFYSTPLTTAVNTPTATSTPFE 120

Query: 121 SCNISPSESCRISRELNPDEYLLEYSADATYLNGESSKKSWTRKLKHSSLGLKLKASRAY 180
           SCNISPSESCRISRELNPDEYLLEYSADATYLNGESSKKSWTRKLKHSSLGLKLKASRAY
Sbjct: 121 SCNISPSESCRISRELNPDEYLLEYSADATYLNGESSKKSWTRKLKHSSLGLKLKASRAY 180

Query: 181 IKSLFTKSGCSDESCAAATKIADEGSASKAHECSKKCAKVEKRKPLGQIPSSIANFNKEK 240
           IKSLFTKSGCSDESCAAATKIADEGSASKAHECSKKCAKVEKRKPLGQIPSSIANFNKEK
Sbjct: 181 IKSLFTKSGCSDESCAAATKIADEGSASKAHECSKKCAKVEKRKPLGQIPSSIANFNKEK 240

Query: 241 GNTENRDRNRRSFSMTIKRQPDIKPSSSASLHTSSSSYSHNSSAYFLKRCGSVNSEIENS 300
           GNTENRDRNRRSFSMTIKRQPDIKPSSSASLHTSSSSYSHNSSAYFLKRCGSVNSEIENS
Sbjct: 241 GNTENRDRNRRSFSMTIKRQPDIKPSSSASLHTSSSSYSHNSSAYFLKRCGSVNSEIENS 300

Query: 301 IQGAIAHCKQSHSQQPFRSRKTVSEVGIYLLSSSSLAVSGDQEIPELCRG 350
           IQGAIAHCKQSHSQQPFRSRKTVSEVGIYLLSSSSLAVSGDQEIPELCRG
Sbjct: 301 IQGAIAHCKQSHSQQPFRSRKTVSEVGIYLLSSSSLAVSGDQEIPELCRG 350

BLAST of MC09g1222 vs. NCBI nr
Match: XP_023527329.1 (probable membrane-associated kinase regulator 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 563 bits (1452), Expect = 2.93e-200
Identity = 294/350 (84.00%), Postives = 319/350 (91.14%), Query Frame = 0

Query: 1   MAVDIPSYDPADDDYIDMEVSSYTNFLCHSKSSPPRPREFEFQMSLNSRGREPTTSPADE 60
           MA DIPSYDP+DDDYIDME+ SYTNFLC SKSSP + REFEFQMS+NSRGREPTTSPADE
Sbjct: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVEKLLQKSNSDYDCRKDNFEEFYSTPLTTAVNTPTATSTPFE 120
           LFYNGKLLPLHHPPRLQMVE+LLQKS+SDYD RKD FEEF+STPLTT++NTPTATSTPF+
Sbjct: 61  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 120

Query: 121 SCNISPSESCRISRELNPDEYLLEYSADATYLNGESSKKSWTRKLKHSSLGLKLKASRAY 180
           SCNISPSES RISRELNPDEY LEYSADA+YLNGESSKKSWT++LK S+LGLKLKASRAY
Sbjct: 121 SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKRLKRSTLGLKLKASRAY 180

Query: 181 IKSLFTKSGCSDESCAAATKIADEGSASKAHECSKKCAKVEKRKPLGQIPSSIANFNKEK 240
           IKSLFTK+GCSDESCA A KIADEGSASKA+ECSKK AKVEKRKP G I SSI+N NK+K
Sbjct: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240

Query: 241 GNTENRDRNRRSFSMTIKRQPDIKPSSSASLHTSSSSYSHNSSAYFLKRCGSVNSEIENS 300
           GNTE+   NRRSFS+ IKRQP+IKPSS ASL+ +SSS  +NSS Y+LKRCGSVNSEIENS
Sbjct: 241 GNTESSSSNRRSFSLVIKRQPNIKPSS-ASLNPTSSSCPNNSSGYYLKRCGSVNSEIENS 300

Query: 301 IQGAIAHCKQSHSQQPFRSRKTVSEVGIYLLSSSSLAVSGDQEIPELCRG 350
           IQGAIAHCKQS SQQP RSRKTVSEVGIYLLSSSSL VS DQE PELCRG
Sbjct: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 349

BLAST of MC09g1222 vs. NCBI nr
Match: KAG6601024.1 (putative membrane-associated kinase regulator 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031828.1 putative membrane-associated kinase regulator 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 563 bits (1450), Expect = 5.91e-200
Identity = 294/350 (84.00%), Postives = 318/350 (90.86%), Query Frame = 0

Query: 1   MAVDIPSYDPADDDYIDMEVSSYTNFLCHSKSSPPRPREFEFQMSLNSRGREPTTSPADE 60
           MA DIPSYDP+DDDYIDME+ SYTNFLC SKSSP + REFEFQMS+NSRGREPTTSPADE
Sbjct: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVEKLLQKSNSDYDCRKDNFEEFYSTPLTTAVNTPTATSTPFE 120
           LFYNGKLLPLHHPPRLQMVE+LLQKS+SDYD RKD FEEF+STPLTT++NTPTATSTPF+
Sbjct: 61  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 120

Query: 121 SCNISPSESCRISRELNPDEYLLEYSADATYLNGESSKKSWTRKLKHSSLGLKLKASRAY 180
           SCNISPSES RISRELNPDEY LEYSADA+YLNGESSKKSWT+KLK S+LGLKLKASRAY
Sbjct: 121 SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAY 180

Query: 181 IKSLFTKSGCSDESCAAATKIADEGSASKAHECSKKCAKVEKRKPLGQIPSSIANFNKEK 240
           IKSLFTK+GCSDESCA A KIADEGSASKA+ECSKK AKVEKRKP G I SSI+N NK+K
Sbjct: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240

Query: 241 GNTENRDRNRRSFSMTIKRQPDIKPSSSASLHTSSSSYSHNSSAYFLKRCGSVNSEIENS 300
           GNTE+   NRRSFS+ IKRQP+IKPSS ASL+ + SS  +NSS Y+LKRCGSVNSEIENS
Sbjct: 241 GNTESSSSNRRSFSLVIKRQPNIKPSS-ASLNPTPSSCPNNSSGYYLKRCGSVNSEIENS 300

Query: 301 IQGAIAHCKQSHSQQPFRSRKTVSEVGIYLLSSSSLAVSGDQEIPELCRG 350
           IQGAIAHCKQS SQQP RSRKTVSEVGIYLLSSSSL VS DQE PELCRG
Sbjct: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 349

BLAST of MC09g1222 vs. NCBI nr
Match: XP_022956992.1 (probable membrane-associated kinase regulator 4 [Cucurbita moschata])

HSP 1 Score: 562 bits (1448), Expect = 1.19e-199
Identity = 294/350 (84.00%), Postives = 318/350 (90.86%), Query Frame = 0

Query: 1   MAVDIPSYDPADDDYIDMEVSSYTNFLCHSKSSPPRPREFEFQMSLNSRGREPTTSPADE 60
           MA DIPSYDP+DDDYIDME+ SYTNFLC SKSSP + REFEFQMS+NSRGREPTTSPADE
Sbjct: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVEKLLQKSNSDYDCRKDNFEEFYSTPLTTAVNTPTATSTPFE 120
           LFYNGKLLPLHHPPRLQMVE+LLQKS+SDYD RKD FEEF+STPLTT++NTPTATSTPF+
Sbjct: 61  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 120

Query: 121 SCNISPSESCRISRELNPDEYLLEYSADATYLNGESSKKSWTRKLKHSSLGLKLKASRAY 180
           SCNISPSES RISRELNPDEY LEYSADA+YLNGESSKKSWT+KLK S+LGLKLKASRAY
Sbjct: 121 SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAY 180

Query: 181 IKSLFTKSGCSDESCAAATKIADEGSASKAHECSKKCAKVEKRKPLGQIPSSIANFNKEK 240
           IKSLFTK+GCSDESCA A KIADEGSASKA+ECSKK AKVEKRKP G I SSI+N NK+K
Sbjct: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240

Query: 241 GNTENRDRNRRSFSMTIKRQPDIKPSSSASLHTSSSSYSHNSSAYFLKRCGSVNSEIENS 300
           GNTE+   NRRSFS+ IKRQP+IKPSS ASL+ + SS  +NSS Y+LKRCGSVNSEIENS
Sbjct: 241 GNTESSRSNRRSFSLVIKRQPNIKPSS-ASLNPTPSSCPNNSSGYYLKRCGSVNSEIENS 300

Query: 301 IQGAIAHCKQSHSQQPFRSRKTVSEVGIYLLSSSSLAVSGDQEIPELCRG 350
           IQGAIAHCKQS SQQP RSRKTVSEVGIYLLSSSSL VS DQE PELCRG
Sbjct: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 349

BLAST of MC09g1222 vs. NCBI nr
Match: XP_038893327.1 (probable membrane-associated kinase regulator 4 [Benincasa hispida])

HSP 1 Score: 550 bits (1417), Expect = 6.03e-195
Identity = 295/350 (84.29%), Postives = 312/350 (89.14%), Query Frame = 0

Query: 1   MAVDIPSYDPADDDYIDMEVSSYTNFLCHSKSSPPRPREFEFQMSLNSRGREPTTSPADE 60
           MAVDIPS DP+DDDYIDME+ SYTNF C SKSSP + REFEFQMSLNSRGREPTTSPADE
Sbjct: 1   MAVDIPSCDPSDDDYIDMEIGSYTNFHCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVEKLLQKSNSDYDCRKDNFEEFYSTPLTTAVNTPTATSTPFE 120
           LFYNGKLLPLHHPPRLQM+EKLLQKS+SDYD RKD FEEFY+TPL+T+VNTPT  STPFE
Sbjct: 61  LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLSTSVNTPT--STPFE 120

Query: 121 SCNISPSESCRISRELNPDEYLLEYSADATYLNGESSKKSWTRKLKHSSLGLKLKASRAY 180
           SCNISPSES RISRELNPDEYLLEYSAD +YLNGES KKSWT+KLKHS+L LKLKASRAY
Sbjct: 121 SCNISPSESFRISRELNPDEYLLEYSADVSYLNGESLKKSWTKKLKHSTLCLKLKASRAY 180

Query: 181 IKSLFTKSGCSDESCAAATKIADEGSASKAHECSKKCAKVEKRKPLGQIPSSIANFNKEK 240
           IKSLFTKSGCSDESCAAA KIADEGSASKA+E SKK AKVEKRKP G I SSIAN NK+K
Sbjct: 181 IKSLFTKSGCSDESCAAAAKIADEGSASKANEYSKKSAKVEKRKPFGPISSSIANSNKDK 240

Query: 241 GNTENRDRNRRSFSMTIKRQPDIKPSSSASLHTSSSSYSHNSSAYFLKRCGSVNSEIENS 300
           GNTEN  RNRRSFSM IKRQP+IK  SSA  + SSSS  + SS  +LKRCGSVNSEIENS
Sbjct: 241 GNTENSSRNRRSFSMVIKRQPNIKSPSSAPQYPSSSSCPNISSRNYLKRCGSVNSEIENS 300

Query: 301 IQGAIAHCKQSHSQQPFRSRKTVSEVGIYLLSSSSLAVSGDQEIPELCRG 350
           IQGAIAHCKQS SQQP RSRKTVSEVGIYLLSSSSL VS DQEIPELCRG
Sbjct: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEIPELCRG 348

BLAST of MC09g1222 vs. ExPASy TrEMBL
Match: A0A6J1CCE6 (probable membrane-associated kinase regulator 4 OS=Momordica charantia OX=3673 GN=LOC111010211 PE=4 SV=1)

HSP 1 Score: 682 bits (1759), Expect = 2.47e-247
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0

Query: 1   MAVDIPSYDPADDDYIDMEVSSYTNFLCHSKSSPPRPREFEFQMSLNSRGREPTTSPADE 60
           MAVDIPSYDPADDDYIDMEVSSYTNFLCHSKSSPPRPREFEFQMSLNSRGREPTTSPADE
Sbjct: 1   MAVDIPSYDPADDDYIDMEVSSYTNFLCHSKSSPPRPREFEFQMSLNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVEKLLQKSNSDYDCRKDNFEEFYSTPLTTAVNTPTATSTPFE 120
           LFYNGKLLPLHHPPRLQMVEKLLQKSNSDYDCRKDNFEEFYSTPLTTAVNTPTATSTPFE
Sbjct: 61  LFYNGKLLPLHHPPRLQMVEKLLQKSNSDYDCRKDNFEEFYSTPLTTAVNTPTATSTPFE 120

Query: 121 SCNISPSESCRISRELNPDEYLLEYSADATYLNGESSKKSWTRKLKHSSLGLKLKASRAY 180
           SCNISPSESCRISRELNPDEYLLEYSADATYLNGESSKKSWTRKLKHSSLGLKLKASRAY
Sbjct: 121 SCNISPSESCRISRELNPDEYLLEYSADATYLNGESSKKSWTRKLKHSSLGLKLKASRAY 180

Query: 181 IKSLFTKSGCSDESCAAATKIADEGSASKAHECSKKCAKVEKRKPLGQIPSSIANFNKEK 240
           IKSLFTKSGCSDESCAAATKIADEGSASKAHECSKKCAKVEKRKPLGQIPSSIANFNKEK
Sbjct: 181 IKSLFTKSGCSDESCAAATKIADEGSASKAHECSKKCAKVEKRKPLGQIPSSIANFNKEK 240

Query: 241 GNTENRDRNRRSFSMTIKRQPDIKPSSSASLHTSSSSYSHNSSAYFLKRCGSVNSEIENS 300
           GNTENRDRNRRSFSMTIKRQPDIKPSSSASLHTSSSSYSHNSSAYFLKRCGSVNSEIENS
Sbjct: 241 GNTENRDRNRRSFSMTIKRQPDIKPSSSASLHTSSSSYSHNSSAYFLKRCGSVNSEIENS 300

Query: 301 IQGAIAHCKQSHSQQPFRSRKTVSEVGIYLLSSSSLAVSGDQEIPELCRG 350
           IQGAIAHCKQSHSQQPFRSRKTVSEVGIYLLSSSSLAVSGDQEIPELCRG
Sbjct: 301 IQGAIAHCKQSHSQQPFRSRKTVSEVGIYLLSSSSLAVSGDQEIPELCRG 350

BLAST of MC09g1222 vs. ExPASy TrEMBL
Match: A0A6J1GXX8 (probable membrane-associated kinase regulator 4 OS=Cucurbita moschata OX=3662 GN=LOC111458502 PE=4 SV=1)

HSP 1 Score: 562 bits (1448), Expect = 5.77e-200
Identity = 294/350 (84.00%), Postives = 318/350 (90.86%), Query Frame = 0

Query: 1   MAVDIPSYDPADDDYIDMEVSSYTNFLCHSKSSPPRPREFEFQMSLNSRGREPTTSPADE 60
           MA DIPSYDP+DDDYIDME+ SYTNFLC SKSSP + REFEFQMS+NSRGREPTTSPADE
Sbjct: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVEKLLQKSNSDYDCRKDNFEEFYSTPLTTAVNTPTATSTPFE 120
           LFYNGKLLPLHHPPRLQMVE+LLQKS+SDYD RKD FEEF+STPLTT++NTPTATSTPF+
Sbjct: 61  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 120

Query: 121 SCNISPSESCRISRELNPDEYLLEYSADATYLNGESSKKSWTRKLKHSSLGLKLKASRAY 180
           SCNISPSES RISRELNPDEY LEYSADA+YLNGESSKKSWT+KLK S+LGLKLKASRAY
Sbjct: 121 SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAY 180

Query: 181 IKSLFTKSGCSDESCAAATKIADEGSASKAHECSKKCAKVEKRKPLGQIPSSIANFNKEK 240
           IKSLFTK+GCSDESCA A KIADEGSASKA+ECSKK AKVEKRKP G I SSI+N NK+K
Sbjct: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240

Query: 241 GNTENRDRNRRSFSMTIKRQPDIKPSSSASLHTSSSSYSHNSSAYFLKRCGSVNSEIENS 300
           GNTE+   NRRSFS+ IKRQP+IKPSS ASL+ + SS  +NSS Y+LKRCGSVNSEIENS
Sbjct: 241 GNTESSRSNRRSFSLVIKRQPNIKPSS-ASLNPTPSSCPNNSSGYYLKRCGSVNSEIENS 300

Query: 301 IQGAIAHCKQSHSQQPFRSRKTVSEVGIYLLSSSSLAVSGDQEIPELCRG 350
           IQGAIAHCKQS SQQP RSRKTVSEVGIYLLSSSSL VS DQE PELCRG
Sbjct: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 349

BLAST of MC09g1222 vs. ExPASy TrEMBL
Match: A0A6J1JSI7 (probable membrane-associated kinase regulator 4 OS=Cucurbita maxima OX=3661 GN=LOC111489379 PE=4 SV=1)

HSP 1 Score: 550 bits (1416), Expect = 4.15e-195
Identity = 292/350 (83.43%), Postives = 314/350 (89.71%), Query Frame = 0

Query: 1   MAVDIPSYDPADDDYIDMEVSSYTNFLCHSKSSPPRPREFEFQMSLNSRGREPTTSPADE 60
           MA DIPS DP+DDDYIDME+ SYTNFLC SKSSP + REFEFQMS+NSRGREPTTSPADE
Sbjct: 1   MAFDIPSSDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVEKLLQKSNSDYDCRKDNFEEFYSTPLTTAVNTPTATSTPFE 120
           LFYNGKLLPLHHPPRLQMVEKL QKS+SDYD RKD FEEF STPLTT++NTPTATSTPF+
Sbjct: 61  LFYNGKLLPLHHPPRLQMVEKLWQKSSSDYDFRKDTFEEF-STPLTTSINTPTATSTPFD 120

Query: 121 SCNISPSESCRISRELNPDEYLLEYSADATYLNGESSKKSWTRKLKHSSLGLKLKASRAY 180
           SCNISPSES RISRELN DEY LEYSADA+YLNGESSKKSWT+KLK S+LGLKLKASR Y
Sbjct: 121 SCNISPSESFRISRELNSDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRTY 180

Query: 181 IKSLFTKSGCSDESCAAATKIADEGSASKAHECSKKCAKVEKRKPLGQIPSSIANFNKEK 240
           IKSLFTK+GCSDESCA A KIADEGSASKA+ECSKK AKVEKRKP G I SSI+N NK+K
Sbjct: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240

Query: 241 GNTENRDRNRRSFSMTIKRQPDIKPSSSASLHTSSSSYSHNSSAYFLKRCGSVNSEIENS 300
           GNTE+   NRRSFS+ IKRQP+IKPSS ASL+ +SSS  +NSS Y+LKRCGSVNSEIENS
Sbjct: 241 GNTESSSSNRRSFSLVIKRQPNIKPSS-ASLNPTSSSCPNNSSGYYLKRCGSVNSEIENS 300

Query: 301 IQGAIAHCKQSHSQQPFRSRKTVSEVGIYLLSSSSLAVSGDQEIPELCRG 350
           IQGAIAHCKQS SQQP RSRKTVSEVGIYLLSSSSL VS DQE PELCRG
Sbjct: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 348

BLAST of MC09g1222 vs. ExPASy TrEMBL
Match: A0A1W6RZ58 (Membrane-associated kinase regulator 4 OS=Cucurbita pepo OX=3663 GN=MAKR4 PE=2 SV=1)

HSP 1 Score: 547 bits (1409), Expect = 4.65e-194
Identity = 288/350 (82.29%), Postives = 312/350 (89.14%), Query Frame = 0

Query: 1   MAVDIPSYDPADDDYIDMEVSSYTNFLCHSKSSPPRPREFEFQMSLNSRGREPTTSPADE 60
           MAVDIPSYD ADDDYIDME+  Y+NFLCHSKSSP + REFEFQMS N+R REPTTSPADE
Sbjct: 1   MAVDIPSYDSADDDYIDMEIGPYSNFLCHSKSSPRQSREFEFQMSSNTREREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVEKLLQKSNSDYDCRKDNFEEFYSTPLTTAVNTPTATSTPFE 120
           LFYNGKLLPLHHPPRLQMVEKLLQKS+SDYD R D FEEFYSTP +TAVN+PTATSTP E
Sbjct: 61  LFYNGKLLPLHHPPRLQMVEKLLQKSSSDYDYRIDIFEEFYSTPFSTAVNSPTATSTPLE 120

Query: 121 SCNISPSESCRISRELNPDEYLLEYSADATYLNGESSKKSWTRKLKHSSLGLKLKASRAY 180
           SCNISPSESC+ISRELNPDEYLLEYS+D +YLNGESSKKSWT+KLKHSSL LKLKASRAY
Sbjct: 121 SCNISPSESCQISRELNPDEYLLEYSSDTSYLNGESSKKSWTKKLKHSSLCLKLKASRAY 180

Query: 181 IKSLFTKSGCSDESCAAATKIADEGSASKAHECSKKCAKVEKRKPLGQIPSSIANFNKEK 240
           IKSLFTKSGCS+ES AAATKIADEG ASKA+ECSKK AK+EKRKPLGQIP SIAN NKEK
Sbjct: 181 IKSLFTKSGCSEESTAAATKIADEGLASKANECSKKGAKLEKRKPLGQIPISIANSNKEK 240

Query: 241 GNTENRDRNRRSFSMTIKRQPDIKPSSSASLHTSSSSYSHNSSAYFLKRCGSVNSEIENS 300
           GN    D NRRSFS+ IKRQP+IKPSSSA+LH +SSS  ++S  YF K CG +NSE ENS
Sbjct: 241 GNM---DHNRRSFSLAIKRQPNIKPSSSATLHPTSSSCPNDSKGYFQKVCGGMNSEFENS 300

Query: 301 IQGAIAHCKQSHSQQPFRSRKTVSEVGIYLLSSSSLAVSGDQEIPELCRG 350
           +QGAIAHCKQS SQQP  S+KTVSEVGIYLLSSS+LAVS DQEIPELCRG
Sbjct: 301 VQGAIAHCKQSQSQQPLHSKKTVSEVGIYLLSSSTLAVSDDQEIPELCRG 347

BLAST of MC09g1222 vs. ExPASy TrEMBL
Match: A0A5A7V1W4 (Putative membrane-associated kinase regulator 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold321G00070 PE=4 SV=1)

HSP 1 Score: 542 bits (1397), Expect = 3.24e-192
Identity = 289/350 (82.57%), Postives = 310/350 (88.57%), Query Frame = 0

Query: 1   MAVDIPSYDPADDDYIDMEVSSYTNFLCHSKSSPPRPREFEFQMSLNSRGREPTTSPADE 60
           MAVDIPS DP+DDDYID+E+ SYTNF C SKSSP + REFEFQMSLNSRGREPTTSPADE
Sbjct: 1   MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVEKLLQKSNSDYDCRKDNFEEFYSTPLTTAVNTPTATSTPFE 120
           LFYNGKLLPLHHPPRLQM+EKLLQKS+SDYD RKD FEEFY+TPLTT++NTPT  STPF+
Sbjct: 61  LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSLNTPT--STPFD 120

Query: 121 SCNISPSESCRISRELNPDEYLLEYSADATYLNGESSKKSWTRKLKHSSLGLKLKASRAY 180
           SCNISPSES RISRELNPDEYLLEYSAD +YLNGE SKKSWT+KLKHS+L LKLKASRAY
Sbjct: 121 SCNISPSESFRISRELNPDEYLLEYSADVSYLNGERSKKSWTKKLKHSTLCLKLKASRAY 180

Query: 181 IKSLFTKSGCSDESCAAATKIADEGSASKAHECSKKCAKVEKRKPLGQIPSSIANFNKEK 240
           IKS FTKSGCSDESCAAA KIADEGSASK +ECSKK A+VEKRKP G I SSIAN NK+K
Sbjct: 181 IKSFFTKSGCSDESCAAAAKIADEGSASKPNECSKKGARVEKRKPFGPISSSIANSNKDK 240

Query: 241 GNTENRDRNRRSFSMTIKRQPDIKPSSSASLHTSSSSYSHNSSAYFLKRCGSVNSEIENS 300
           GNTEN  RNRRSFS+ IKRQP IK SS A LH +SSS  + SS  +LKRCGSVNSEIENS
Sbjct: 241 GNTENSSRNRRSFSLVIKRQPIIKSSSFAPLHPTSSSCPNISSGKYLKRCGSVNSEIENS 300

Query: 301 IQGAIAHCKQSHSQQPFRSRKTVSEVGIYLLSSSSLAVSGDQEIPELCRG 350
           IQGAIAHCKQS SQQP RSRKTVSEVGIYLLSSS+L VS DQEIPEL RG
Sbjct: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG 348

BLAST of MC09g1222 vs. TAIR 10
Match: AT2G39370.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G37380.1); Has 184 Blast hits to 178 proteins in 53 species: Archae - 0; Bacteria - 58; Metazoa - 9; Fungi - 0; Plants - 103; Viruses - 0; Other Eukaryotes - 14 (source: NCBI BLink). )

HSP 1 Score: 252.3 bits (643), Expect = 5.7e-67
Identity = 173/358 (48.32%), Postives = 222/358 (62.01%), Query Frame = 0

Query: 1   MAVDIPSYDPADDDYIDMEVSSYTNFLCHSKSSPPRPREFEFQMS-LNSRGREPTTSPAD 60
           MA  +   D  ++DYIDMEV+S+TN L     S   PREFEFQMS L     + TTSPAD
Sbjct: 1   MAAYLERCDSVEEDYIDMEVTSFTN-LVRKTLSNNYPREFEFQMSHLCPLEIDKTTSPAD 60

Query: 61  ELFYNGKLLPLHHPPRLQMVEKLLQKSNSDYDCRKDNFEEFYSTPLTT-AVNTPTATSTP 120
           ELFY GKLLPLH PPRLQMV+K+L+    D        +EFYSTPL T  V TP  ++TP
Sbjct: 61  ELFYKGKLLPLHLPPRLQMVQKILEDYTFD--------DEFYSTPLATGTVTTPVTSNTP 120

Query: 121 FESCNISPSESCRISRELNPDEYLLEYSADATYLNGESSKKSWTRKL---KHSSLGLKLK 180
           FESC +SP+ESC++S+ELNP++Y LEYS        +  KKSWT KL   K SSLG K+K
Sbjct: 121 FESCTVSPAESCQVSKELNPEDYFLEYSDSLE--EDDEKKKSWTTKLRLMKQSSLGTKIK 180

Query: 181 ASRAYIKSLFTKSGCSDE-SCA-AATKIADEGSASKAHECSKKCAKVEKRKPLGQIPSSI 240
           ASRAY++S F K+ CSDE SCA +A ++ADE S            +  + KP GQI +  
Sbjct: 181 ASRAYLRSFFGKTSCSDESSCASSAARVADEDSV----------LRYSRVKPFGQIKTER 240

Query: 241 ANFNKEKGNTENRDRNRRSFSMTIKRQPDIKPSSSASLHTSSSSYSHNSSAYFLKRCGSV 300
               K++ N      +RRSFS++++RQ     ++ +S + SS+S        FLKR  S 
Sbjct: 241 P---KKQSNGSVSGSHRRSFSVSMRRQ-----AAKSSNNKSSNSLGFR-PLQFLKRSTSS 300

Query: 301 NSEIENSIQGAIAHCKQSHSQ-QPFRSRKTVSEVGIYLLSSSSLAVSGDQEIPELCRG 351
           +SEIENSIQGAI HCKQS  Q Q  +   TV+EVG   LS+S +A   DQE  ++ RG
Sbjct: 301 SSEIENSIQGAILHCKQSQQQKQKQKQYSTVNEVGFCSLSASRIAARDDQEWAQMFRG 328

BLAST of MC09g1222 vs. TAIR 10
Match: AT2G37380.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G39370.1); Has 1284 Blast hits to 422 proteins in 114 species: Archae - 0; Bacteria - 90; Metazoa - 125; Fungi - 151; Plants - 136; Viruses - 0; Other Eukaryotes - 782 (source: NCBI BLink). )

HSP 1 Score: 141.4 bits (355), Expect = 1.4e-33
Identity = 135/352 (38.35%), Postives = 180/352 (51.14%), Query Frame = 0

Query: 12  DDDYIDMEV----------SSYTNFLCHSKSSPPRPREFEFQMSLNS-RGREPTTSPADE 71
           DD YIDMEV          SS + F     SSPP+ REFEFQM  ++    E TTSPADE
Sbjct: 18  DDGYIDMEVNLSSSSSSSTSSSSFFSFPVTSSPPQSREFEFQMCSSAVASGESTTSPADE 77

Query: 72  LFYNGKLLPLHHPPRLQMVEKLLQKSNSDYDCRKDNFEEFYSTPLTTAVNTPTATSTPFE 131
           LFY G+LLPLH PPRL+MV+KLL  S+S     +        TP++        +   F 
Sbjct: 78  LFYKGQLLPLHLPPRLKMVQKLLLASSSSTAATE--------TPISPRAAADVLSPRRFS 137

Query: 132 SCNISPSESC--RISRELNPDEYLLEYSADATYLNGESSKKSWTRKLKHSSLGLKLKASR 191
           SC I   E+C   IS EL   +  +E        N      SW++K+KHSS+  KLKASR
Sbjct: 138 SCEIGQDENCFFEISTEL---KRFIE-------SNENHLGNSWSKKIKHSSITQKLKASR 197

Query: 192 AYIKSLFTKSGCSDESCAAATKIADEGSASKAHECSKKCAKVEKRKPLGQIPSSIANFNK 251
           AYIK+LF+K  CSD S                 E + +  K+E        PS ++    
Sbjct: 198 AYIKALFSKQACSDSS-----------------EINPR-FKIE--------PSKVSRKKN 257

Query: 252 EKGNTENRDR-NRRSFSMTIKRQPDIKPSSSASLHTSSSSYSHNSSAYFLKRCGSVNSEI 311
              N+EN    +RRSFS  I+R    K S+S+S   SSSS S  SS++     GS++ + 
Sbjct: 258 PFVNSENPLLIHRRSFSGVIQRHSQAKCSTSSS---SSSSASSLSSSFSFGSNGSLDLQT 317

Query: 312 --------ENSIQGAIAHCKQSHSQQPFRSRKTVSEVGIYLLSSSSLAVSGD 342
                   +NSI+GAI HCKQS + +  +S  T SE+     S +S++  GD
Sbjct: 318 LMRSSNASDNSIEGAIEHCKQSFTTR--KSNVTESEL---CSSRTSVSTCGD 317

BLAST of MC09g1222 vs. TAIR 10
Match: AT5G26230.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 47.4 bits (111), Expect = 2.8e-05
Identity = 89/346 (25.72%), Postives = 134/346 (38.73%), Query Frame = 0

Query: 31  KSSPPRPR---------------EFEFQMSLNSRGREPTTSPADELFYNGKLLPLHHPPR 90
           +  PPRPR               EFEF +S++ R    +  PADELFY G+LLPL   PR
Sbjct: 2   RRQPPRPRNSPPQSHSSPSSSSSEFEFNISISPRKASSSLCPADELFYKGQLLPLQLSPR 61

Query: 91  LQMVEKL-LQKSNSDYDCRKDNFEEFYSTPLTTAVNTPTATSTPFESCNISPSESCRISR 150
           L +V  L    S+SDY     +     +   +T  N+ T ++  F   +  P + C  SR
Sbjct: 62  LSLVRTLGSSTSSSDYTSSSSSSVATSAARDSTESNSSTDSTASFPLLHPPPLDCCDSSR 121

Query: 151 ELNPDEYLLEYSADATYLNGESSKKSWTRKLKHSSLGLKL-KASRAYIKSLFTKSGCSDE 210
             +  +       D  +       KS       SS G  L + S  + K   T       
Sbjct: 122 PSSVTD-------DEDFFFKPPKNKS-------SSGGFSLSRFSSVFKKDPKTNLHHHSS 181

Query: 211 SCAAATKIADEGSASKAHECSKKCAK--VEKRKPLGQ--IPSSIANFNKEKGNT-ENRDR 270
           S + AT  A   S  +    +K+  +  ++K KPL +   P   +N   E  ++ ++   
Sbjct: 182 SSSTATTAAAPSSVKRMSSTAKEVIRKYMKKVKPLYEKLSPKQSSNIKTESSSSLKDSGN 241

Query: 271 NRRSFS--MTIKRQPDI-------------------KPSSSASLHTSSSSYSHNSSAYFL 316
           N R  +   T+   P +                   K    A+   SS   S N S    
Sbjct: 242 NIRGTTTVTTVTAAPTVVSSGGGLSISFSGNLMKYTKRGRCAASCPSSMRSSPNHSGVLT 301

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O806248.1e-6648.32Probable membrane-associated kinase regulator 4 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9ZUS82.0e-3238.35Probable membrane-associated kinase regulator 3 OS=Arabidopsis thaliana OX=3702 ... [more]
Q3E9363.9e-0425.72Probable membrane-associated kinase regulator 1 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
XP_022139254.15.11e-247100.00probable membrane-associated kinase regulator 4 [Momordica charantia][more]
XP_023527329.12.93e-20084.00probable membrane-associated kinase regulator 4 [Cucurbita pepo subsp. pepo][more]
KAG6601024.15.91e-20084.00putative membrane-associated kinase regulator 4, partial [Cucurbita argyrosperma... [more]
XP_022956992.11.19e-19984.00probable membrane-associated kinase regulator 4 [Cucurbita moschata][more]
XP_038893327.16.03e-19584.29probable membrane-associated kinase regulator 4 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1CCE62.47e-247100.00probable membrane-associated kinase regulator 4 OS=Momordica charantia OX=3673 G... [more]
A0A6J1GXX85.77e-20084.00probable membrane-associated kinase regulator 4 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1JSI74.15e-19583.43probable membrane-associated kinase regulator 4 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A1W6RZ584.65e-19482.29Membrane-associated kinase regulator 4 OS=Cucurbita pepo OX=3663 GN=MAKR4 PE=2 S... [more]
A0A5A7V1W43.24e-19282.57Putative membrane-associated kinase regulator 4 OS=Cucumis melo var. makuwa OX=1... [more]
Match NameE-valueIdentityDescription
AT2G39370.15.7e-6748.32unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT2G37380.11.4e-3338.35unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT5G26230.12.8e-0525.72unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 259..279
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 228..279
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 240..257
NoneNo IPR availablePANTHERPTHR33312:SF21MEMBRANE-ASSOCIATED KINASE REGULATOR 3-RELATEDcoord: 2..349
IPR039620BKI1/Probable membrane-associated kinase regulator 1/3/4PANTHERPTHR33312MEMBRANE-ASSOCIATED KINASE REGULATOR 4-RELATEDcoord: 2..349

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g1222.1MC09g1222.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0016310 phosphorylation
cellular_component GO:0005886 plasma membrane
molecular_function GO:0016301 kinase activity
molecular_function GO:0019210 kinase inhibitor activity