MC09g1103 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC09g1103
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	gibberellin 2-beta-dioxygenase 2
Location	MC09: 16940689 .. 16942759 (-)
RNA-Seq Expression	MC09g1103
Synteny	MC09g1103

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGTGATAAATGTTGATGTTGACATTTTCGTCTCTGTTATCAACTCTGAACTTTATTTATTGATTATTCATACATTTTATATATTTATAAAATTCAGGTTTAAATTTAAATACGCTAATTCAATTAACCGTGTTCTCGTTGATTTTCTCCTCTTCTCTTCTCTTCTCTTCTCATGGCTTCCTCAGTCGATCACAAGCAAATCTATGGCGCCGGCACCACCGCAGCTCCGCCGCCAACGCCGTCGTCACAAACCAGTCACCTCTCCACCTCCGCCGCCGCCGATGCTCTCTCGCGCCTCCTCCACCGCCTCCCGCCCAATCTCTCCCTCCCTACTCGCCGCTCGCCTTCCGTTAGGTCTCCTCCAACTATTTCCTTTTCAGAAACTTCGAATCCTTCACTTCTCGATCGCCTTCTCTCTGCCTCCTCCGAACTCGGCTTCTTCCAACTCACCGATCACCAAATCTCCTCTCGCCTCGCTCTCTCTGCCGAGTCGGAATCCGCCGCGCTCTTCGGTCTTCCGATGGAGACGAAGGAATCTCTGTTTCCGAAGAACTGGCCGCTCGGATTCAAAGGCGACGGAGATGAAGATTCAGACGGAAGCGGAAACGGCGATTCGTTTTCGTTCTGTCTCGATTCGACGCCGTGCTCCTCCGATTCAGCCGAGATTTCCTTACATTCGTTGCAGGAGTTCGTTCGTGCAATGGAGAAGCTCGGATTGAAGATTGTCGAGTTTCTATCTCGTGCGATTGGCTTCGAGAATCCAATCGGCGAGGATCGTACCAGATTCCGGTCTCTAATATGGATATCGGAAGGTTCACGGAGAGCCGAACCGGCAGTGGCCGGCGGATTCTACCCGTACATCGTGGGATTACAGTACCAGAGCAGAAACCAGAAGTGTTCTCTATTAGGGGATTCCAGCTGGGTTACGGCGGCGGCGGCGGCGGACTCTGTGATGGTCTCCATTGGGGACATTGCGCAAGTAAGTTATCAATACGCTGTCGTTTCGTAGATTCTTTTTTTCTTTAAAAATAGATTTAGAAAGAAAATGAGATAATTACCGAATTTAATTAACGGCCAACGAATTTCCCTAATTAATAATTAAATAAAAAGATATTAGGTTGCACGAATTGAGAGGCAGTGGTCACGTGCTTTTGTGACAAAAATACTTGTTTCACAAAATCTAGACAAGAAAGCTCTCCGTTATTTGTTAAAATAATTGATGGAAATATTCTCATAAGAAGAAAGGAAAAAGAGAGAGAAAAAAGAAGGGGTTGATTCAATGGATTATTATGGGTATGGGTGTAAATTGTGGGTAATTTCTTTTGACCAAATTTAGATAATGGTGGGTAATTTCTTTTGACAGGTGTGGAGCAACGGAAAGCTAAAGAAAGTGAGAGGAAGGCCGGTGGCAGTGGCGGCGGATCCCAACGGCGGCGGCATCTCAATATCGCTACTCATAACTCTTCCGGCCGAAAGCGAAGTTCGTCCTCTAATTACTCCTCCGGCGAGATCCGACCGGCGAGACGAGGCGGCGGAGGAGGAAGAAGAGGAGGTGAAAATGGCGTTTAAATCATTTTGGTTGGAAGAGTACGCGTGGAGAGTGCATCACGATCGGTGCTTTCTCCTCAAAGATCCGCTGGACAGATATCGGATTTGAGATCATTTTCCTTTTCTGAGTCCATCGCCATTGTTGTTTCTCTTCAAACCACAGCTGTGGAGTCTCAAGCCTGAACCGGCCGGTACGTACGTGGTGTGTTCAATTCACTGTTTTTTTTTTCTTTTCTCGCTCTCTCTACCAATTTTCTTTTGAACAGTTTTTATTGCGTATGCTTGTGACAATTTTGTTTCAAATTTAAATAATCCTATGATATATATATAAAAGTTGTCTAAGTCGTTTGCAGAGGGAATAAAAATAAACAAAAGGTGAAAAATATAGAGATTAAAATAGATGTTTTTAAGGTTTTCAAAATGAGGTAAAATGGAAATTACCGTGGTCAAAATAGTATTTAAATCACTTTGATAACATTGTTGATGTTTTTTATTAATATTGTTCAAAAATCATAGATTTTTG

mRNA sequence

TGGTGATAAATGTTGATGTTGACATTTTCGTCTCTGTTATCAACTCTGAACTTTATTTATTGATTATTCATACATTTTATATATTTATAAAATTCAGGTTTAAATTTAAATACGCTAATTCAATTAACCGTGTTCTCGTTGATTTTCTCCTCTTCTCTTCTCTTCTCTTCTCATGGCTTCCTCAGTCGATCACAAGCAAATCTATGGCGCCGGCACCACCGCAGCTCCGCCGCCAACGCCGTCGTCACAAACCAGTCACCTCTCCACCTCCGCCGCCGCCGATGCTCTCTCGCGCCTCCTCCACCGCCTCCCGCCCAATCTCTCCCTCCCTACTCGCCGCTCGCCTTCCGTTAGGTCTCCTCCAACTATTTCCTTTTCAGAAACTTCGAATCCTTCACTTCTCGATCGCCTTCTCTCTGCCTCCTCCGAACTCGGCTTCTTCCAACTCACCGATCACCAAATCTCCTCTCGCCTCGCTCTCTCTGCCGAGTCGGAATCCGCCGCGCTCTTCGGTCTTCCGATGGAGACGAAGGAATCTCTGTTTCCGAAGAACTGGCCGCTCGGATTCAAAGGCGACGGAGATGAAGATTCAGACGGAAGCGGAAACGGCGATTCGTTTTCGTTCTGTCTCGATTCGACGCCGTGCTCCTCCGATTCAGCCGAGATTTCCTTACATTCGTTGCAGGAGTTCGTTCGTGCAATGGAGAAGCTCGGATTGAAGATTGTCGAGTTTCTATCTCGTGCGATTGGCTTCGAGAATCCAATCGGCGAGGATCGTACCAGATTCCGGTCTCTAATATGGATATCGGAAGGTTCACGGAGAGCCGAACCGGCAGTGGCCGGCGGATTCTACCCGTACATCGTGGGATTACAGTACCAGAGCAGAAACCAGAAGTGTTCTCTATTAGGGGATTCCAGCTGGGTTACGGCGGCGGCGGCGGCGGACTCTGTGATGGTCTCCATTGGGGACATTGCGCAAGTGTGGAGCAACGGAAAGCTAAAGAAAGTGAGAGGAAGGCCGGTGGCAGTGGCGGCGGATCCCAACGGCGGCGGCATCTCAATATCGCTACTCATAACTCTTCCGGCCGAAAGCGAAGTTCGTCCTCTAATTACTCCTCCGGCGAGATCCGACCGGCGAGACGAGGCGGCGGAGGAGGAAGAAGAGGAGGTGAAAATGGCGTTTAAATCATTTTGGTTGGAAGAGTACGCGTGGAGAGTGCATCACGATCGGTGCTTTCTCCTCAAAGATCCGCTGGACAGATATCGGATTTGAGATCATTTTCCTTTTCTGAGTCCATCGCCATTGTTGTTTCTCTTCAAACCACAGCTGTGGAGTCTCAAGCCTGAACCGGCCGGTACGTACGTGGTGTGTTCAATTCACTGTTTTTTTTTTCTTTTCTCGCTCTCTCTACCAATTTTCTTTTGAACAGTTTTTATTGCGTATGCTTGTGACAATTTTGTTTCAAATTTAAATAATCCTATGATATATATATAAAAGTTGTCTAAGTCGTTTGCAGAGGGAATAAAAATAAACAAAAGGTGAAAAATATAGAGATTAAAATAGATGTTTTTAAGGTTTTCAAAATGAGGTAAAATGGAAATTACCGTGGTCAAAATAGTATTTAAATCACTTTGATAACATTGTTGATGTTTTTTATTAATATTGTTCAAAAATCATAGATTTTTG

Coding sequence (CDS)

ATGGCTTCCTCAGTCGATCACAAGCAAATCTATGGCGCCGGCACCACCGCAGCTCCGCCGCCAACGCCGTCGTCACAAACCAGTCACCTCTCCACCTCCGCCGCCGCCGATGCTCTCTCGCGCCTCCTCCACCGCCTCCCGCCCAATCTCTCCCTCCCTACTCGCCGCTCGCCTTCCGTTAGGTCTCCTCCAACTATTTCCTTTTCAGAAACTTCGAATCCTTCACTTCTCGATCGCCTTCTCTCTGCCTCCTCCGAACTCGGCTTCTTCCAACTCACCGATCACCAAATCTCCTCTCGCCTCGCTCTCTCTGCCGAGTCGGAATCCGCCGCGCTCTTCGGTCTTCCGATGGAGACGAAGGAATCTCTGTTTCCGAAGAACTGGCCGCTCGGATTCAAAGGCGACGGAGATGAAGATTCAGACGGAAGCGGAAACGGCGATTCGTTTTCGTTCTGTCTCGATTCGACGCCGTGCTCCTCCGATTCAGCCGAGATTTCCTTACATTCGTTGCAGGAGTTCGTTCGTGCAATGGAGAAGCTCGGATTGAAGATTGTCGAGTTTCTATCTCGTGCGATTGGCTTCGAGAATCCAATCGGCGAGGATCGTACCAGATTCCGGTCTCTAATATGGATATCGGAAGGTTCACGGAGAGCCGAACCGGCAGTGGCCGGCGGATTCTACCCGTACATCGTGGGATTACAGTACCAGAGCAGAAACCAGAAGTGTTCTCTATTAGGGGATTCCAGCTGGGTTACGGCGGCGGCGGCGGCGGACTCTGTGATGGTCTCCATTGGGGACATTGCGCAAGTGTGGAGCAACGGAAAGCTAAAGAAAGTGAGAGGAAGGCCGGTGGCAGTGGCGGCGGATCCCAACGGCGGCGGCATCTCAATATCGCTACTCATAACTCTTCCGGCCGAAAGCGAAGTTCGTCCTCTAATTACTCCTCCGGCGAGATCCGACCGGCGAGACGAGGCGGCGGAGGAGGAAGAAGAGGAGGTGAAAATGGCGTTTAAATCATTTTGGTTGGAAGAGTACGCGTGGAGAGTGCATCACGATCGGTGCTTTCTCCTCAAAGATCCGCTGGACAGATATCGGATTTGA

Protein sequence

MASSVDHKQIYGAGTTAAPPPTPSSQTSHLSTSAAADALSRLLHRLPPNLSLPTRRSPSVRSPPTISFSETSNPSLLDRLLSASSELGFFQLTDHQISSRLALSAESESAALFGLPMETKESLFPKNWPLGFKGDGDEDSDGSGNGDSFSFCLDSTPCSSDSAEISLHSLQEFVRAMEKLGLKIVEFLSRAIGFENPIGEDRTRFRSLIWISEGSRRAEPAVAGGFYPYIVGLQYQSRNQKCSLLGDSSWVTAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVAVAADPNGGGISISLLITLPAESEVRPLITPPARSDRRDEAAEEEEEEVKMAFKSFWLEEYAWRVHHDRCFLLKDPLDRYRI

Homology

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BLAST of MC09g1103 vs. ExPASy Swiss-Prot
Match: Q9XFR9 (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 2.5e-09
Identity = 67/283 (23.67%), Postives = 118/283 (41.70%), Query Frame = 0


Query: 52  LPTRRSPSVRSPPTISFSETSNPSLLDRLLSASSELGFFQLTDHQISSRLALSAESESAA 111
           +PT +   V +  +I     ++P    R++ A  E GFF++ +H +   L    E E+  
Sbjct: 17  IPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNHGVRPELMTRLEQEAIG 76

Query: 112 LFGLPMETKESLFPKNWPLGFKGDGDEDSDGSGNGDSFSFCL------DSTPCSS----D 171
            FGLP   K    P   P G+   G++    +G+     + L       S+P +S     
Sbjct: 77  FFGLPQSLKNRAGPPE-PYGY---GNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQ 136

Query: 172 SAEISLHSLQEFVRAMEKLGLKIVEFLSRAIGFENP-------IGEDRTRFRSLIWISEG 231
           + +I   S++E+++ ++++  K++E ++  +G E           E       L      
Sbjct: 137 TPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAA 196

Query: 232 SRRAEPAVAGGF----YPYIVGLQYQSRNQKCSL-LGDSSWVTAAAAADSVMVSIGDIAQ 291
              AE  V  GF     P I+ +   +      + + D SWV       S  +++GD  Q
Sbjct: 197 EEEAEKMVKVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQ 256

Query: 292 VWSNGKLKKVRGRPVAVAADPNGGGISISLLITLPAESEVRPL 313
           V +NG+ K V+ R   V AD     IS+      P   ++ PL
Sbjct: 257 VMTNGRFKSVKHR---VLADTRRSRISMIYFGGPPLSQKIAPL 292

[Close]

BLAST of MC09g1103 vs. ExPASy Swiss-Prot
Match: P31237 (1-aminocyclopropane-1-carboxylate oxidase OS=Actinidia deliciosa OX=3627 GN=ACO PE=2 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 9.8e-06
Identity = 72/302 (23.84%), Postives = 129/302 (42.72%), Query Frame = 0


Query: 77  LDRLLSASSELGFFQLTDHQISSRLALSAESESAALFGLPMET--KESLFPKNWPLGFKG 136
           ++++  A    GFF+L +H IS  L  + E  +   +   ME   KE +  K    G + 
Sbjct: 21  MEKIKDACENWGFFELVNHGISHELMDTVERLTKEHYNKCMEQRFKEMVATK----GLEA 80

Query: 137 DGDEDSDGSGNGDSFSFCLDSTPCSS-----DSAEISLHSLQEFVRAMEKLGLKIVEFLS 196
              E +D        +F L   P S+     D  +    +++EF   +EKL  ++++ L 
Sbjct: 81  VQSEINDLDWES---TFFLRHLPVSNISEIPDLEQDHRKAMKEFAEKLEKLAEQLLDLLC 140

Query: 197 RAIGFENPIGEDRTRFRSLIWISEGSRRA------EPAVAGGFYPY-----IVGLQYQSR 256
             +G E   G  +  F      + G++ +       P +  G   +     I+ L   ++
Sbjct: 141 ENVGLEK--GYLKKAFYGSKGPTFGTKVSNYPPCPRPELIKGLRAHTDAGGIILLFQDNK 200

Query: 257 NQKCSLLGDSSWVTAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVAVAADPNGGGISIS 316
                LL D  W+       S++++IGD  +V +NGK K V  R   V A P+G  +SI+
Sbjct: 201 VSGLQLLKDGEWIDVPPMKHSIVINIGDQLEVITNGKYKSVMHR---VIAQPDGNRMSIA 260

Query: 317 LLITLPAESEVRPLITPPARSDRRDEAAEEEEEEVKMAFKSFWLEEYAWRVHHDRCFLLK 361
                 +++ + P    PA  D+       EE++ K  +  F  E+Y  +++    F  K
Sbjct: 261 SFYNPGSDAVMYP---APALVDK-------EEDQQKQVYPKFVFEDY-MKLYAGLKFQAK 299

[Close]

BLAST of MC09g1103 vs. ExPASy Swiss-Prot
Match: Q7XP65 (Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX6 PE=1 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 1.3e-05
Identity = 69/281 (24.56%), Postives = 108/281 (38.43%), Query Frame = 0


Query: 83  ASSELGFFQLTDHQISSRLALSAESESAALFGLPMETKESLFPKNWPLGFKGDGDEDSDG 142
           A+SE GFFQLT+H +   L      E A LF LP ETKE     N    +          
Sbjct: 76  AASEWGFFQLTNHGVGRELMEEMRREQARLFRLPFETKEKAGLLNGSYRWGNPTATSLRH 135

Query: 143 SGNGDSFSFCLDS---TPCSSDSAEISLHSLQEFVRAMEKLGLKIVEFLSRAIGFEN--- 202
               ++F   L S     C           +QE   AM ++   +   L+  +       
Sbjct: 136 LSWSEAFHVPLASISGADCDFGDLTSLRGVMQEVAEAMSRVANTVAAALAEELTGRGGGG 195

Query: 203 -------PIGEDRTRFRSLIWISEGSRRAEPAVAGGFYPY----IVGLQYQSRNQKCSLL 262
                  P G D T     + ++        A   G  P+     + +  Q +     L+
Sbjct: 196 ASAAPWFPAGCDET--TCFLRLNRYPACPFAADTFGLVPHTDSDFLTVLCQDQVGGLHLM 255

Query: 263 GDSSWVTAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVAVAADPNGGGISISLLITLPA 322
            DS WV      D+++V+IGD+ Q WSN + K V  + VA A       +S++  +    
Sbjct: 256 KDSRWVAVRPRPDALVVNIGDLFQAWSNNRYKSVEHKVVANAKTDR---LSVAYFLCPSY 315

Query: 323 ESEVRPLITP-PARSDRRDEAAEEEEEEVKMAFKSFWLEEY 346
           +S V     P P R+    E  ++ +E+V+   K   L  +
Sbjct: 316 DSLVGTCGEPSPYRAFTFGEYRKKVQEDVRTTGKKIGLPNF 351

[Close]

BLAST of MC09g1103 vs. ExPASy Swiss-Prot
Match: Q9LY48 (Jasmonate-induced oxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=JOX3 PE=1 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 1.3e-05
Identity = 76/320 (23.75%), Postives = 131/320 (40.94%), Query Frame = 0


Query: 21  PTPSSQTSHLSTSAAADALSRLLHRLP--PNLSLPTRRSPSVRSPPTISFSE------TS 80
           P P  +   LS S      +R +  L   PN++   + +P   + P I          T 
Sbjct: 8   PEPIVRVQSLSESNLGAIPNRYVKPLSQRPNITPHNKHNPQTTTIPIIDLGRLYTDDLTL 67

Query: 81  NPSLLDRLLSASSELGFFQLTDHQISSRLALSAESESAALFGLPMETKESLFPKNWPLGF 140
               LD +  A  ELGFFQ+ +H +S +L   A++     F LPME K      N P  +
Sbjct: 68  QAKTLDEISKACRELGFFQVVNHGMSPQLMDQAKATWREFFNLPMELKN--MHANSPKTY 127

Query: 141 KGDGDEDSDGSGNGDSFS--FCLDSTPCS----SDSAEISLHS---LQEFVRAMEKLGLK 200
           +G G       G    +S  + L   P S    +    + LH    L+++ + M KL   
Sbjct: 128 EGYGSRLGVEKGAILDWSDYYYLHYQPSSLKDYTKWPSLPLHCREILEDYCKEMVKLCEN 187

Query: 201 IVEFLSRAIG-----FENPIGEDRTRFRSLIWISEGSRRAEPAVAGGFYPYI--VGLQYQ 260
           +++ LS+ +G      +N  G  +      + ++   +  +P +  G  P+    GL   
Sbjct: 188 LMKILSKNLGLQEDRLQNAFG-GKEESGGCLRVNYYPKCPQPELTLGISPHSDPGGLTIL 247

Query: 261 SRNQKCSLL----GDSSWVTAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVAVAADPNG 313
             +++ + L     D +W+T   A  + +V++GD  Q+ SN   K V  R   V  +P  
Sbjct: 248 LPDEQVASLQVRGSDDAWITVEPAPHAFIVNMGDQIQMLSNSIYKSVEHR---VIVNPEN 307

[Close]

BLAST of MC09g1103 vs. ExPASy Swiss-Prot
Match: Q8S0S6 (Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX3 PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 2.2e-05
Identity = 61/257 (23.74%), Postives = 106/257 (41.25%), Query Frame = 0


Query: 58  PSVRSP-PTISFS-----ETSNPSLLDRLLSASSELGFFQLTDHQISSRLALSAESESAA 117
           P +RSP P   FS     +  +P     ++ A    GFF++ +H +++     AESE+  
Sbjct: 14  PLLRSPDPGDVFSGVPVVDLGSPGAARAVVDACERYGFFKVVNHGVATDTMDKAESEAVR 73

Query: 118 LFGLPMETKESLFPKNWPLGFKGDGDEDSDGSGNGDSFSFCLD----STPCSSDSAEISL 177
            F      K+   P  +P G+       +   G  +     LD    +  C+  S  +  
Sbjct: 74  FFSQTQPDKDRSGPA-YPFGYGSKRIGFNGDMGWLEYLLLALDDASLADACTVPSCAVFR 133

Query: 178 HSLQEFVRAMEKLGLKIVEFLSRAIGFENPIGEDRTRFRSLIWISEGSRRA------EPA 237
            +L E++  + K+ ++++E +S  +G        +    S +  +EGS +        P 
Sbjct: 134 AALNEYISGVRKVAVRVMEAMSEGLGIA------QADALSALVTAEGSDQVFRVNHYPPC 193

Query: 238 VA-GGFYPYIVGLQYQSRNQKCSL------------LGDSSWVTAAAAADSVMVSIGDIA 286
            A  G    + G    +  Q  S+            L D  WV+  +  DS  V++GD  
Sbjct: 194 RALQGLGCSVTGFGEHTDPQLVSVLRSNGTSGLQIALRDGQWVSVPSDRDSFFVNVGDSL 253

[Close]

BLAST of MC09g1103 vs. NCBI nr
Match: XP_022146739.1 (gibberellin 2-beta-dioxygenase 2 [Momordica charantia])

HSP 1 Score: 709 bits (1829), Expect = 3.80e-257
Identity = 366/366 (100.00%), Postives = 366/366 (100.00%), Query Frame = 0


Query: 1   MASSVDHKQIYGAGTTAAPPPTPSSQTSHLSTSAAADALSRLLHRLPPNLSLPTRRSPSV 60
           MASSVDHKQIYGAGTTAAPPPTPSSQTSHLSTSAAADALSRLLHRLPPNLSLPTRRSPSV
Sbjct: 1   MASSVDHKQIYGAGTTAAPPPTPSSQTSHLSTSAAADALSRLLHRLPPNLSLPTRRSPSV 60

Query: 61  RSPPTISFSETSNPSLLDRLLSASSELGFFQLTDHQISSRLALSAESESAALFGLPMETK 120
           RSPPTISFSETSNPSLLDRLLSASSELGFFQLTDHQISSRLALSAESESAALFGLPMETK
Sbjct: 61  RSPPTISFSETSNPSLLDRLLSASSELGFFQLTDHQISSRLALSAESESAALFGLPMETK 120

Query: 121 ESLFPKNWPLGFKGDGDEDSDGSGNGDSFSFCLDSTPCSSDSAEISLHSLQEFVRAMEKL 180
           ESLFPKNWPLGFKGDGDEDSDGSGNGDSFSFCLDSTPCSSDSAEISLHSLQEFVRAMEKL
Sbjct: 121 ESLFPKNWPLGFKGDGDEDSDGSGNGDSFSFCLDSTPCSSDSAEISLHSLQEFVRAMEKL 180

Query: 181 GLKIVEFLSRAIGFENPIGEDRTRFRSLIWISEGSRRAEPAVAGGFYPYIVGLQYQSRNQ 240
           GLKIVEFLSRAIGFENPIGEDRTRFRSLIWISEGSRRAEPAVAGGFYPYIVGLQYQSRNQ
Sbjct: 181 GLKIVEFLSRAIGFENPIGEDRTRFRSLIWISEGSRRAEPAVAGGFYPYIVGLQYQSRNQ 240

Query: 241 KCSLLGDSSWVTAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVAVAADPNGGGISISLL 300
           KCSLLGDSSWVTAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVAVAADPNGGGISISLL
Sbjct: 241 KCSLLGDSSWVTAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVAVAADPNGGGISISLL 300

Query: 301 ITLPAESEVRPLITPPARSDRRDEAAEEEEEEVKMAFKSFWLEEYAWRVHHDRCFLLKDP 360
           ITLPAESEVRPLITPPARSDRRDEAAEEEEEEVKMAFKSFWLEEYAWRVHHDRCFLLKDP
Sbjct: 301 ITLPAESEVRPLITPPARSDRRDEAAEEEEEEVKMAFKSFWLEEYAWRVHHDRCFLLKDP 360

Query: 361 LDRYRI 366
           LDRYRI
Sbjct: 361 LDRYRI 366

[Close]

BLAST of MC09g1103 vs. NCBI nr
Match: XP_038892860.1 (gibberellin 2-beta-dioxygenase 6 [Benincasa hispida])

HSP 1 Score: 510 bits (1314), Expect = 2.79e-178
Identity = 283/384 (73.70%), Postives = 310/384 (80.73%), Query Frame = 0


Query: 1   MASSVDHKQ---IYGAGTTAAPPPTPSSQTSHLSTSAAADALSRLLHRLPPNLSLPTRRS 60
           MASS+DH Q   I+G G TAAPPPTPSSQT+HLSTSAAADALSRLLHRLPPNLSLPTRR 
Sbjct: 16  MASSLDHNQLPNIHG-GATAAPPPTPSSQTNHLSTSAAADALSRLLHRLPPNLSLPTRRF 75

Query: 61  PSVRSPPTISFSETSNPSLLDRLLSASSELGFFQLTDHQISSRLALSAESESAALFGLPM 120
           PSVRSPPTISFSE+ NP LLDRLLSA+SELGFFQLTDH+ISS LALSAESE+AALF LP 
Sbjct: 76  PSVRSPPTISFSESPNPDLLDRLLSAASELGFFQLTDHKISSHLALSAESEAAALFDLPA 135

Query: 121 ETKESLFPKNWPLGFKGDGDEDSDGSGNGDSFSFCLDSTPCSSDSAEISLHSLQEFVRAM 180
           E KESLFPKNWPLG+KGDGDE+SDG G     SFC D  PC SDSAEISLHSL+EFV  M
Sbjct: 136 EKKESLFPKNWPLGYKGDGDEESDGIGE----SFCFDLRPCLSDSAEISLHSLKEFVLKM 195

Query: 181 EKLGLKIVEFLSRAIGFENPIGEDRTRFRSLIWISEGSRRAEPAVAGGFYPYIVGLQYQS 240
           E LGLKI+EFL RAIGFENPIGEDRT FRSLIWISEG R  EPA+A GFYPYIVGLQYQS
Sbjct: 196 ESLGLKILEFLFRAIGFENPIGEDRTGFRSLIWISEGCRSTEPAMADGFYPYIVGLQYQS 255

Query: 241 RNQKCSLLGDSSWVTAAAAA-DSVMVSIGDIAQVWSNGKL-KKVRGRPVAVAAD--PNGG 300
           +NQ+CSLLGDS WV AAAAA DSVMVSIGDIAQVWSNGKL KKVRGRPV  A     N  
Sbjct: 256 KNQRCSLLGDSGWVAAAAAATDSVMVSIGDIAQVWSNGKLPKKVRGRPVPTANTNGANSH 315

Query: 301 GISISLLITLPAESEVRPLITPPA-----------RSDRRDEAAEEEEEEVKMAFKSFWL 360
            IS+SLLITLP +S+V PL+ P +            + ++ E  + +E + K  F SF  
Sbjct: 316 AISLSLLITLPVDSQVSPLLLPSSVNADVEQFRSVTAKKKKEDDDNDERKEKAMFHSFSF 375

Query: 361 EEYAWRVHHDRCFLLKDPLDRYRI 366
           EEYAWRV+HDRCFLLKDPL+RYRI
Sbjct: 376 EEYAWRVYHDRCFLLKDPLNRYRI 394

[Close]

BLAST of MC09g1103 vs. NCBI nr
Match: XP_011654997.1 (gibberellin 2-beta-dioxygenase 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 509 bits (1311), Expect = 4.50e-178
Identity = 276/382 (72.25%), Postives = 305/382 (79.84%), Query Frame = 0


Query: 1   MASSVDHKQIYG--AGTTAAPPPTPSSQTSHLSTSAAADALSRLLHRLPPNLSLPTRRSP 60
           MASS+DH Q+    AG TAAPPPTPSSQT+HLSTSAAADALS+LLHRLPPNLSLPTRRSP
Sbjct: 1   MASSLDHNQLPNIHAGATAAPPPTPSSQTNHLSTSAAADALSKLLHRLPPNLSLPTRRSP 60

Query: 61  SVRSPPTISFSETSNPSLLDRLLSASSELGFFQLTDHQISSRLALSAESESAALFGLPME 120
           SV  PPTISFSE+ NP LL+RLLSA+SELGFFQLTDH+ISS LALSAESESA LF LP E
Sbjct: 61  SVIPPPTISFSESPNPDLLNRLLSAASELGFFQLTDHKISSHLALSAESESAPLFNLPAE 120

Query: 121 TKESLFPKNWPLGFKGDGDEDSDGSGNGDSFSFCLDSTPCSSDSAEISLHSLQEFVRAME 180
            KESLFPKNWPLGFKGDGDE+SDGSG     S C DS  C SDS EIS HSL +FV  ME
Sbjct: 121 KKESLFPKNWPLGFKGDGDEESDGSGE----SLCFDSRNCLSDSPEISFHSLTDFVLEME 180

Query: 181 KLGLKIVEFLSRAIGFENPIGEDRTRFRSLIWISEGSRRAEPAVAGGFYPYIVGLQYQSR 240
            LGLKIVEFL RAIGFENPIGEDRT FRSL+WISEG R  EPA+AGGFYPYI+GLQYQSR
Sbjct: 181 SLGLKIVEFLFRAIGFENPIGEDRTGFRSLVWISEGCRSTEPAMAGGFYPYIIGLQYQSR 240

Query: 241 NQKCSLLGDSSWVTAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVAVAAD------PNG 300
           NQKCSLLGDS WV AAAAADSVMVSIGDIAQVWSNGKLKK+RGRPV +A+        N 
Sbjct: 241 NQKCSLLGDSGWVAAAAAADSVMVSIGDIAQVWSNGKLKKMRGRPVPMASSVANTSSTNS 300

Query: 301 GGISISLLITLPAESEVRPLITPPARSDRRDEAAEEEEEE--------VKMAFKSFWLEE 360
             IS+SLLITLP +++V PL+     +   ++  ++E E+         K  F SF  EE
Sbjct: 301 RTISLSLLITLPVDTQVSPLLLSTNENANEEQFDKKEREDDGDSGEGKEKAVFHSFNFEE 360

Query: 361 YAWRVHHDRCFLLKDPLDRYRI 366
           YAWRV+HDRCFLLKDPL+RYRI
Sbjct: 361 YAWRVYHDRCFLLKDPLNRYRI 378

[Close]

BLAST of MC09g1103 vs. NCBI nr
Match: XP_008465218.1 (PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase [Cucumis melo])

HSP 1 Score: 505 bits (1300), Expect = 2.36e-176
Identity = 277/386 (71.76%), Postives = 310/386 (80.31%), Query Frame = 0


Query: 1   MASSVDHKQ---IYGAGTTAAPPPTPSSQTSHLSTSAAADALSRLLHRLPPNLSLPTRRS 60
           MASS++H Q   I+G G TAAPPPTPSSQT+HLSTSAAADALS+LLHRLPPNLSLPTRRS
Sbjct: 1   MASSLNHNQLPNIHG-GATAAPPPTPSSQTNHLSTSAAADALSKLLHRLPPNLSLPTRRS 60

Query: 61  PSVRSPPTISFSETSNPSLLDRLLSASSELGFFQLTDHQISSRLALSAESESAALFGLPM 120
           PSV +PPTISFSE+ NP LL+ LLSA+SELGFFQLTDH+ISS LALSAESESAALF L  
Sbjct: 61  PSVIAPPTISFSESPNPDLLNHLLSAASELGFFQLTDHKISSHLALSAESESAALFNLSA 120

Query: 121 ETKESLFPKNWPLGFKGDGDEDSDGSGNGDSFSFCLDSTPCSSDSAEISLHSLQEFVRAM 180
           E KESLFPKNWPLGFKGDGDE+SDGSG+    S C DS  C SDSAEISLHSL EFV  M
Sbjct: 121 EKKESLFPKNWPLGFKGDGDEESDGSGD----SLCFDSRLCFSDSAEISLHSLTEFVLEM 180

Query: 181 EKLGLKIVEFLSRAIGFENPIGEDRTRFRSLIWISEGSRRAEPAVAGGFYPYIVGLQYQS 240
           E LGLKIVEFL RAIGFENPIGEDRT FRSL+WISEG R  EPA+AGGFYPYIVGLQYQS
Sbjct: 181 ESLGLKIVEFLFRAIGFENPIGEDRTGFRSLVWISEGCRGTEPAMAGGFYPYIVGLQYQS 240

Query: 241 RNQKCSLLGDSSWVTAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVAVAAD------PN 300
           RNQ+CSLLGDS WV AAAAADSVMVSIGD+AQVWSNGKLKK+RGRPV +A+        N
Sbjct: 241 RNQRCSLLGDSGWVAAAAAADSVMVSIGDVAQVWSNGKLKKMRGRPVPMASSLANTSSTN 300

Query: 301 GGGISISLLITLPAESEVRPLITPPARSD-----------RRDEAAEEEEEEVKMAFKSF 360
              IS+SLLITLP +++V PL++                 +++E A+ +E + K  F SF
Sbjct: 301 SRTISLSLLITLPVDTQVSPLLSTNENEKEEQFASVRDKMKKEEDADNDEGKEKAVFHSF 360

Query: 361 WLEEYAWRVHHDRCFLLKDPLDRYRI 366
             EEYAWRV+ DRCFLLKDPL+RYRI
Sbjct: 361 NFEEYAWRVYPDRCFLLKDPLNRYRI 381

[Close]

BLAST of MC09g1103 vs. NCBI nr
Match: XP_022943276.1 (gibberellin 3-beta-dioxygenase 4 [Cucurbita moschata] >KAG6599990.1 Gibberellin 2-beta-dioxygenase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030665.1 Gibberellin 2-beta-dioxygenase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 444 bits (1142), Expect = 1.59e-152
Identity = 255/376 (67.82%), Postives = 288/376 (76.60%), Query Frame = 0


Query: 1   MASSVDHKQIYG--AGTTAAPPPTPSSQTSHLSTSAAADALSRLLHRLPPNLSLPTRRSP 60
           MASSVDH ++     G TAAPPPTPSSQT+HLSTSAAA+ALSRLLHRLPP LSLPTRRS 
Sbjct: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 60

Query: 61  SVRSPPTISFSETSNPSLLDRLLSASSELGFFQLTDHQISSRLALSAESESAALFGLPME 120
           SV SPPTISFS++ +     RLLSA SE GFFQ+TDH+ISS L LSAESESAALF +  E
Sbjct: 61  SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE 120

Query: 121 TKESLFPKNWPLGFKGDGDEDSDGSGNGDSFSFCLDSTPCSSDSAEISLHSLQEFVRAME 180
            KE+LFPK+W  GF GD DE+SDG+G     SFC D   C   S EI L +LQE V AME
Sbjct: 121 KKEALFPKDW--GFIGDEDEESDGAGE----SFCFDPRLCLPYSDEICLQALQELVPAME 180

Query: 181 KLGLKIVEFLSRAIGFENPIGEDRTRFRSLIWISEGSRRAEPAVAGGFYPYIVGLQYQSR 240
            LGLKIVE L RAIG ENPIGEDR+RFRSLIWIS+GSR AEPAV  GFYPYIVGL YQS+
Sbjct: 181 YLGLKIVEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSK 240

Query: 241 NQKCSLLGDSSWV-TAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVAVAAD--PNGGGI 300
           NQ+CS+LGDS WV TAA AADSVMVSIGDIAQVWSNGK+KKVRGR VA AAD   N G I
Sbjct: 241 NQRCSVLGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTI 300

Query: 301 SISLLITLPAESEVRPLITPPARSDRRDEAAEE-----EEEEVKMAFKSFWLEEYAWRVH 360
           SISLLITLP +++V PL+ PP   +   EA E+     E E+    F+SF  EEYAW+V+
Sbjct: 301 SISLLITLPLDTQVSPLL-PPTDENTDAEAEEDGGGGNEAEKKTAVFRSFSFEEYAWKVY 360

Query: 361 HDRCFLLKDPLDRYRI 366
            +RCFLL+DPL  YRI
Sbjct: 361 QNRCFLLRDPLAGYRI 369

[Close]

BLAST of MC09g1103 vs. ExPASy TrEMBL
Match: A0A6J1CYY0 (gibberellin 2-beta-dioxygenase 2 OS=Momordica charantia OX=3673 GN=LOC111015875 PE=4 SV=1)

HSP 1 Score: 709 bits (1829), Expect = 1.84e-257
Identity = 366/366 (100.00%), Postives = 366/366 (100.00%), Query Frame = 0


Query: 1   MASSVDHKQIYGAGTTAAPPPTPSSQTSHLSTSAAADALSRLLHRLPPNLSLPTRRSPSV 60
           MASSVDHKQIYGAGTTAAPPPTPSSQTSHLSTSAAADALSRLLHRLPPNLSLPTRRSPSV
Sbjct: 1   MASSVDHKQIYGAGTTAAPPPTPSSQTSHLSTSAAADALSRLLHRLPPNLSLPTRRSPSV 60

Query: 61  RSPPTISFSETSNPSLLDRLLSASSELGFFQLTDHQISSRLALSAESESAALFGLPMETK 120
           RSPPTISFSETSNPSLLDRLLSASSELGFFQLTDHQISSRLALSAESESAALFGLPMETK
Sbjct: 61  RSPPTISFSETSNPSLLDRLLSASSELGFFQLTDHQISSRLALSAESESAALFGLPMETK 120

Query: 121 ESLFPKNWPLGFKGDGDEDSDGSGNGDSFSFCLDSTPCSSDSAEISLHSLQEFVRAMEKL 180
           ESLFPKNWPLGFKGDGDEDSDGSGNGDSFSFCLDSTPCSSDSAEISLHSLQEFVRAMEKL
Sbjct: 121 ESLFPKNWPLGFKGDGDEDSDGSGNGDSFSFCLDSTPCSSDSAEISLHSLQEFVRAMEKL 180

Query: 181 GLKIVEFLSRAIGFENPIGEDRTRFRSLIWISEGSRRAEPAVAGGFYPYIVGLQYQSRNQ 240
           GLKIVEFLSRAIGFENPIGEDRTRFRSLIWISEGSRRAEPAVAGGFYPYIVGLQYQSRNQ
Sbjct: 181 GLKIVEFLSRAIGFENPIGEDRTRFRSLIWISEGSRRAEPAVAGGFYPYIVGLQYQSRNQ 240

Query: 241 KCSLLGDSSWVTAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVAVAADPNGGGISISLL 300
           KCSLLGDSSWVTAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVAVAADPNGGGISISLL
Sbjct: 241 KCSLLGDSSWVTAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVAVAADPNGGGISISLL 300

Query: 301 ITLPAESEVRPLITPPARSDRRDEAAEEEEEEVKMAFKSFWLEEYAWRVHHDRCFLLKDP 360
           ITLPAESEVRPLITPPARSDRRDEAAEEEEEEVKMAFKSFWLEEYAWRVHHDRCFLLKDP
Sbjct: 301 ITLPAESEVRPLITPPARSDRRDEAAEEEEEEVKMAFKSFWLEEYAWRVHHDRCFLLKDP 360

Query: 361 LDRYRI 366
           LDRYRI
Sbjct: 361 LDRYRI 366

[Close]

BLAST of MC09g1103 vs. ExPASy TrEMBL
Match: A0A1S3CNS4 (1-aminocyclopropane-1-carboxylate oxidase OS=Cucumis melo OX=3656 GN=LOC103502873 PE=4 SV=1)

HSP 1 Score: 505 bits (1300), Expect = 1.14e-176
Identity = 277/386 (71.76%), Postives = 310/386 (80.31%), Query Frame = 0


Query: 1   MASSVDHKQ---IYGAGTTAAPPPTPSSQTSHLSTSAAADALSRLLHRLPPNLSLPTRRS 60
           MASS++H Q   I+G G TAAPPPTPSSQT+HLSTSAAADALS+LLHRLPPNLSLPTRRS
Sbjct: 1   MASSLNHNQLPNIHG-GATAAPPPTPSSQTNHLSTSAAADALSKLLHRLPPNLSLPTRRS 60

Query: 61  PSVRSPPTISFSETSNPSLLDRLLSASSELGFFQLTDHQISSRLALSAESESAALFGLPM 120
           PSV +PPTISFSE+ NP LL+ LLSA+SELGFFQLTDH+ISS LALSAESESAALF L  
Sbjct: 61  PSVIAPPTISFSESPNPDLLNHLLSAASELGFFQLTDHKISSHLALSAESESAALFNLSA 120

Query: 121 ETKESLFPKNWPLGFKGDGDEDSDGSGNGDSFSFCLDSTPCSSDSAEISLHSLQEFVRAM 180
           E KESLFPKNWPLGFKGDGDE+SDGSG+    S C DS  C SDSAEISLHSL EFV  M
Sbjct: 121 EKKESLFPKNWPLGFKGDGDEESDGSGD----SLCFDSRLCFSDSAEISLHSLTEFVLEM 180

Query: 181 EKLGLKIVEFLSRAIGFENPIGEDRTRFRSLIWISEGSRRAEPAVAGGFYPYIVGLQYQS 240
           E LGLKIVEFL RAIGFENPIGEDRT FRSL+WISEG R  EPA+AGGFYPYIVGLQYQS
Sbjct: 181 ESLGLKIVEFLFRAIGFENPIGEDRTGFRSLVWISEGCRGTEPAMAGGFYPYIVGLQYQS 240

Query: 241 RNQKCSLLGDSSWVTAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVAVAAD------PN 300
           RNQ+CSLLGDS WV AAAAADSVMVSIGD+AQVWSNGKLKK+RGRPV +A+        N
Sbjct: 241 RNQRCSLLGDSGWVAAAAAADSVMVSIGDVAQVWSNGKLKKMRGRPVPMASSLANTSSTN 300

Query: 301 GGGISISLLITLPAESEVRPLITPPARSD-----------RRDEAAEEEEEEVKMAFKSF 360
              IS+SLLITLP +++V PL++                 +++E A+ +E + K  F SF
Sbjct: 301 SRTISLSLLITLPVDTQVSPLLSTNENEKEEQFASVRDKMKKEEDADNDEGKEKAVFHSF 360

Query: 361 WLEEYAWRVHHDRCFLLKDPLDRYRI 366
             EEYAWRV+ DRCFLLKDPL+RYRI
Sbjct: 361 NFEEYAWRVYPDRCFLLKDPLNRYRI 381

[Close]

BLAST of MC09g1103 vs. ExPASy TrEMBL
Match: A0A6J1FRA7 (gibberellin 3-beta-dioxygenase 4 OS=Cucurbita moschata OX=3662 GN=LOC111448055 PE=4 SV=1)

HSP 1 Score: 444 bits (1142), Expect = 7.69e-153
Identity = 255/376 (67.82%), Postives = 288/376 (76.60%), Query Frame = 0


Query: 1   MASSVDHKQIYG--AGTTAAPPPTPSSQTSHLSTSAAADALSRLLHRLPPNLSLPTRRSP 60
           MASSVDH ++     G TAAPPPTPSSQT+HLSTSAAA+ALSRLLHRLPP LSLPTRRS 
Sbjct: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 60

Query: 61  SVRSPPTISFSETSNPSLLDRLLSASSELGFFQLTDHQISSRLALSAESESAALFGLPME 120
           SV SPPTISFS++ +     RLLSA SE GFFQ+TDH+ISS L LSAESESAALF +  E
Sbjct: 61  SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE 120

Query: 121 TKESLFPKNWPLGFKGDGDEDSDGSGNGDSFSFCLDSTPCSSDSAEISLHSLQEFVRAME 180
            KE+LFPK+W  GF GD DE+SDG+G     SFC D   C   S EI L +LQE V AME
Sbjct: 121 KKEALFPKDW--GFIGDEDEESDGAGE----SFCFDPRLCLPYSDEICLQALQELVPAME 180

Query: 181 KLGLKIVEFLSRAIGFENPIGEDRTRFRSLIWISEGSRRAEPAVAGGFYPYIVGLQYQSR 240
            LGLKIVE L RAIG ENPIGEDR+RFRSLIWIS+GSR AEPAV  GFYPYIVGL YQS+
Sbjct: 181 YLGLKIVEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSK 240

Query: 241 NQKCSLLGDSSWV-TAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVAVAAD--PNGGGI 300
           NQ+CS+LGDS WV TAA AADSVMVSIGDIAQVWSNGK+KKVRGR VA AAD   N G I
Sbjct: 241 NQRCSVLGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTI 300

Query: 301 SISLLITLPAESEVRPLITPPARSDRRDEAAEE-----EEEEVKMAFKSFWLEEYAWRVH 360
           SISLLITLP +++V PL+ PP   +   EA E+     E E+    F+SF  EEYAW+V+
Sbjct: 301 SISLLITLPLDTQVSPLL-PPTDENTDAEAEEDGGGGNEAEKKTAVFRSFSFEEYAWKVY 360

Query: 361 HDRCFLLKDPLDRYRI 366
            +RCFLL+DPL  YRI
Sbjct: 361 QNRCFLLRDPLAGYRI 369

[Close]

BLAST of MC09g1103 vs. ExPASy TrEMBL
Match: A0A6J1JL54 (uncharacterized protein LOC111485581 OS=Cucurbita maxima OX=3661 GN=LOC111485581 PE=4 SV=1)

HSP 1 Score: 437 bits (1123), Expect = 1.86e-148
Identity = 253/375 (67.47%), Postives = 285/375 (76.00%), Query Frame = 0


Query: 1   MASSVDHKQIYG--AGTTAAPPPTPSSQTSHLSTSAAADALSRLLHRLPPNLSLPTRRSP 60
           MASSVDH ++     G TAAPPPTPSSQT+HLSTSAAA+ALSRLLHRLPP LSLPTRRS 
Sbjct: 103 MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 162

Query: 61  SVRSPPTISFSETSNPSLLDRLLSASSELGFFQLTDHQISSRLALSAESESAALFGLPME 120
           SV SPPTISFSE+ +     RLLSA SE GFFQ+TDH+ISS L LSA SESAALF +  E
Sbjct: 163 SVISPPTISFSESPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAVSESAALFDIQAE 222

Query: 121 TKESLFPKNWPLGFKGDGDEDSDGSGNGDSFSFCLDSTPCSSDSAEISLHSLQEFVRAME 180
            KE+LFPK+W  GF GD DE+SDG+G     SFC D   C   S EI L +LQE V AME
Sbjct: 223 KKEALFPKDW--GFIGDEDEESDGAGE----SFCFDPRLCLPYSDEICLQALQELVPAME 282

Query: 181 KLGLKIVEFLSRAIGFENPIGEDRTRFRSLIWISEGSRRAEPAVAGGFYPYIVGLQYQSR 240
            LGLKIVE L RAIG ENPIGE R+RFRSLIW S+GSR AEPAV  GFYPYIVGL YQS+
Sbjct: 283 YLGLKIVEPLFRAIGLENPIGEGRSRFRSLIWTSKGSRSAEPAVTCGFYPYIVGLHYQSK 342

Query: 241 NQKCSLLGDSSWV-TAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVAVAAD--PNGGGI 300
           NQ+CS+LGDS WV TAA AADSVMVSIGDIAQVWSNGK+KKVRGR VA AAD   N G I
Sbjct: 343 NQRCSVLGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTI 402

Query: 301 SISLLITLPAESEVRPLITPPARSDRRDEAAEE----EEEEVKMAFKSFWLEEYAWRVHH 360
           SISLLITLP +++V PL+ PP   +   EA E+    E E+    F+SF  EEYAW+V+ 
Sbjct: 403 SISLLITLPLDTQVSPLL-PPTDENTDAEAEEDGGGNEVEKKTAVFRSFSFEEYAWKVYQ 462

Query: 361 DRCFLLKDPLDRYRI 366
           +RCFLL+DPL  YRI
Sbjct: 463 NRCFLLRDPLAGYRI 470

[Close]

BLAST of MC09g1103 vs. ExPASy TrEMBL
Match: A0A0A0KP52 (DIOX_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G208470 PE=4 SV=1)

HSP 1 Score: 412 bits (1059), Expect = 3.92e-141
Identity = 220/272 (80.88%), Postives = 234/272 (86.03%), Query Frame = 0


Query: 1   MASSVDHKQIYG--AGTTAAPPPTPSSQTSHLSTSAAADALSRLLHRLPPNLSLPTRRSP 60
           MASS+DH Q+    AG TAAPPPTPSSQT+HLSTSAAADALS+LLHRLPPNLSLPTRRSP
Sbjct: 1   MASSLDHNQLPNIHAGATAAPPPTPSSQTNHLSTSAAADALSKLLHRLPPNLSLPTRRSP 60

Query: 61  SVRSPPTISFSETSNPSLLDRLLSASSELGFFQLTDHQISSRLALSAESESAALFGLPME 120
           SV  PPTISFSE+ NP LL+RLLSA+SELGFFQLTDH+ISS LALSAESESA LF LP E
Sbjct: 61  SVIPPPTISFSESPNPDLLNRLLSAASELGFFQLTDHKISSHLALSAESESAPLFNLPAE 120

Query: 121 TKESLFPKNWPLGFKGDGDEDSDGSGNGDSFSFCLDSTPCSSDSAEISLHSLQEFVRAME 180
            KESLFPKNWPLGFKGDGDE+SDGSG     S C DS  C SDS EIS HSL +FV  ME
Sbjct: 121 KKESLFPKNWPLGFKGDGDEESDGSGE----SLCFDSRNCLSDSPEISFHSLTDFVLEME 180

Query: 181 KLGLKIVEFLSRAIGFENPIGEDRTRFRSLIWISEGSRRAEPAVAGGFYPYIVGLQYQSR 240
            LGLKIVEFL RAIGFENPIGEDRT FRSL+WISEG R  EPA+AGGFYPYI+GLQYQSR
Sbjct: 181 SLGLKIVEFLFRAIGFENPIGEDRTGFRSLVWISEGCRSTEPAMAGGFYPYIIGLQYQSR 240

Query: 241 NQKCSLLGDSSWVTAAAAADSVMVSIGDIAQV 270
           NQKCSLLGDS WV AAAAADSVMVSIGDIAQ+
Sbjct: 241 NQKCSLLGDSGWVAAAAAADSVMVSIGDIAQL 268

[Close]

BLAST of MC09g1103 vs. TAIR 10
Match: AT3G11150.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 278.5 bits (711), Expect = 7.8e-75
Identity = 175/383 (45.69%), Postives = 242/383 (63.19%), Query Frame = 0


Query: 2   ASSVDHKQ--IYGAGTTAAPPPTPSSQTSHLSTSA----AADALSRLLHRLPPNLSLPTR 61
           ++ ++H Q  I   G TAAPPPTPS   SHL+T++    A DALS L HRLPP LSLP R
Sbjct: 5   SAHLNHHQPLIKHGGATAAPPPTPS--CSHLNTTSKSETAVDALSSLFHRLPPLLSLPNR 64

Query: 62  RSPSVRSPPTISFSETSNPSLLDRLLSASSELGFFQL----TDHQISSRLALSAESESAA 121
           R  S+ S P +S S   +    D L+SA ++ G+FQL    +D      +A +AES+S +
Sbjct: 65  R--SIPSLPMVSLS-AGDRLEWDDLISAVTDFGYFQLINDDSDILFPPGIAEAAESDSLS 124

Query: 122 LFGLPMETKESLFPKNWPLGFKGDGDEDSDGSGNGDSFSFCLDSTPCSSDSAEISLHSLQ 181
           L  L  E KES FPK WPLG++ D +            SFCLD+  CS+DS+E++L SL+
Sbjct: 125 LLELSEEKKESSFPKKWPLGYEADAETP----------SFCLDA-DCSTDSSELNLSSLR 184

Query: 182 EFVRAMEKLGLKIVEFLSRAIGFENPIGEDRTRFRSLIWISEGSRRAEPAVAGGFYPYIV 241
           EF R +EK+GLK VE L+ A+GF    G D TRF +L+W+++G    EP V  GFYP++V
Sbjct: 185 EFTRTLEKVGLKTVEMLANALGF----GYDSTRFNTLMWVNQGVPDDEPEVTNGFYPFVV 244

Query: 242 GLQYQSRNQKCSLLGDSSWVTAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVAVAADPN 301
            LQYQ R QK  LL +S WV+     DSV+V++GDIAQVW NG++K+V+ RPV  +   +
Sbjct: 245 CLQYQIREQKYCLLTESGWVSVLPRVDSVLVTLGDIAQVWRNGEVKRVKYRPVLCSGQKD 304

Query: 302 G--GGISISLLITLPAESEVRPLITPPARSDRRDEAAEEEEE------EVKMAFKSFWLE 361
           G    ++++L++TLP +S V  L    +  D+ +E AEEEEE      + + AF+ F  +
Sbjct: 305 GPVKCVTMTLMLTLPMDSMVSSLKDMISDGDKEEEYAEEEEEDGGARSDERRAFRPFSFK 364

Query: 362 EYAWRVHHDRCFLLKDPLDRYRI 367
           EYAWRV+ +R F  +DPLDRYRI
Sbjct: 365 EYAWRVYQERLF-FRDPLDRYRI 366

[Close]

BLAST of MC09g1103 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 64.7 bits (156), Expect = 1.8e-10
Identity = 67/283 (23.67%), Postives = 118/283 (41.70%), Query Frame = 0


Query: 52  LPTRRSPSVRSPPTISFSETSNPSLLDRLLSASSELGFFQLTDHQISSRLALSAESESAA 111
           +PT +   V +  +I     ++P    R++ A  E GFF++ +H +   L    E E+  
Sbjct: 17  IPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNHGVRPELMTRLEQEAIG 76

Query: 112 LFGLPMETKESLFPKNWPLGFKGDGDEDSDGSGNGDSFSFCL------DSTPCSS----D 171
            FGLP   K    P   P G+   G++    +G+     + L       S+P +S     
Sbjct: 77  FFGLPQSLKNRAGPPE-PYGY---GNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQ 136

Query: 172 SAEISLHSLQEFVRAMEKLGLKIVEFLSRAIGFENP-------IGEDRTRFRSLIWISEG 231
           + +I   S++E+++ ++++  K++E ++  +G E           E       L      
Sbjct: 137 TPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAA 196

Query: 232 SRRAEPAVAGGF----YPYIVGLQYQSRNQKCSL-LGDSSWVTAAAAADSVMVSIGDIAQ 291
              AE  V  GF     P I+ +   +      + + D SWV       S  +++GD  Q
Sbjct: 197 EEEAEKMVKVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQ 256

Query: 292 VWSNGKLKKVRGRPVAVAADPNGGGISISLLITLPAESEVRPL 313
           V +NG+ K V+ R   V AD     IS+      P   ++ PL
Sbjct: 257 VMTNGRFKSVKHR---VLADTRRSRISMIYFGGPPLSQKIAPL 292

[Close]

BLAST of MC09g1103 vs. TAIR 10
Match: AT3G55970.1 (jasmonate-regulated gene 21 )

HSP 1 Score: 52.4 bits (124), Expect = 9.1e-07
Identity = 76/320 (23.75%), Postives = 131/320 (40.94%), Query Frame = 0


Query: 21  PTPSSQTSHLSTSAAADALSRLLHRLP--PNLSLPTRRSPSVRSPPTISFSE------TS 80
           P P  +   LS S      +R +  L   PN++   + +P   + P I          T 
Sbjct: 8   PEPIVRVQSLSESNLGAIPNRYVKPLSQRPNITPHNKHNPQTTTIPIIDLGRLYTDDLTL 67

Query: 81  NPSLLDRLLSASSELGFFQLTDHQISSRLALSAESESAALFGLPMETKESLFPKNWPLGF 140
               LD +  A  ELGFFQ+ +H +S +L   A++     F LPME K      N P  +
Sbjct: 68  QAKTLDEISKACRELGFFQVVNHGMSPQLMDQAKATWREFFNLPMELKN--MHANSPKTY 127

Query: 141 KGDGDEDSDGSGNGDSFS--FCLDSTPCS----SDSAEISLHS---LQEFVRAMEKLGLK 200
           +G G       G    +S  + L   P S    +    + LH    L+++ + M KL   
Sbjct: 128 EGYGSRLGVEKGAILDWSDYYYLHYQPSSLKDYTKWPSLPLHCREILEDYCKEMVKLCEN 187

Query: 201 IVEFLSRAIG-----FENPIGEDRTRFRSLIWISEGSRRAEPAVAGGFYPYI--VGLQYQ 260
           +++ LS+ +G      +N  G  +      + ++   +  +P +  G  P+    GL   
Sbjct: 188 LMKILSKNLGLQEDRLQNAFG-GKEESGGCLRVNYYPKCPQPELTLGISPHSDPGGLTIL 247

Query: 261 SRNQKCSLL----GDSSWVTAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVAVAADPNG 313
             +++ + L     D +W+T   A  + +V++GD  Q+ SN   K V  R   V  +P  
Sbjct: 248 LPDEQVASLQVRGSDDAWITVEPAPHAFIVNMGDQIQMLSNSIYKSVEHR---VIVNPEN 307

[Close]

BLAST of MC09g1103 vs. TAIR 10
Match: AT4G23340.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 52.0 bits (123), Expect = 1.2e-06
Identity = 62/256 (24.22%), Postives = 94/256 (36.72%), Query Frame = 0


Query: 64  PTISFSETSNPSLLDRLLSASSELGFFQLTDHQISSRLALSAESESAALFGLPMETKESL 123
           P +  ++    S+L  L  A  E GFF +T+H IS  +     S S  +F  P+E+K  L
Sbjct: 11  PVLDLTQPIESSILSSLSEACKEWGFFYVTNHGISKEMFSKICSLSRDVFKAPLESKLKL 70

Query: 124 FPKNWPLGFKGDGDEDSDGSGNGDSFSFCLDSTPCSSDSAEISL------HSLQEFVRAM 183
            P ++   +                F   + S P  SDSA+ S       H   E    M
Sbjct: 71  GPISYTPRYIA-----------SPYFESLVVSGPDFSDSAKASADVLFQDHHKPELRETM 130

Query: 184 EKLGLKIVEFLSRAI-------------------GFENPIG----------EDRTRFRSL 243
           ++ G K+ E   R I                    F N  G           D  +   L
Sbjct: 131 QEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEEL 190

Query: 244 IWISEGSRRAEPAVAGGFYPYIVGLQYQSRNQKCSLLGDSSWVTAAAAADSVMVSIGDIA 285
           +    G       +   +   + GLQ +S+  K        W+      D ++V+IGD+ 
Sbjct: 191 V-EGLGMHTDMSCITIVYQDSVGGLQMRSKEGK--------WIDINPCNDFLVVNIGDLM 246

[Close]

BLAST of MC09g1103 vs. TAIR 10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )

HSP 1 Score: 50.8 bits (120), Expect = 2.6e-06
Identity = 57/239 (23.85%), Postives = 97/239 (40.59%), Query Frame = 0


Query: 70  ETSNPSLLDRLLSASSELGFFQLTDHQISSRLALSAESESAALFGLPMETKESLFPKNWP 129
           + S+P     L+ A  + GFF++ +H +S+ L    E E+   F LP   K  +    +P
Sbjct: 22  DMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQV--AGYP 81

Query: 130 LGFKG-----DGD---------EDSDGSGNGDSFSFCLDSTPCSSDSAEISLHSLQEFV- 189
            G+       +GD           +  SG+G  F   L S     ++ E    S+++   
Sbjct: 82  FGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTTSVRKMTF 141

Query: 190 ----RAMEKLGLKIVEFLSRAIGFENPIGEDRTRFRSLIWISEGSRRAEPAVAG-GFY-- 249
               +  + LG+K    LS+ +  +N     R        +S         V G G +  
Sbjct: 142 DVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVIGFGEHTD 201

Query: 250 PYIVGLQYQSRNQKCSL-LGDSSWVTAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVA 286
           P I+ +   +      + L D SW++      S   ++GD  QV +NG+ K VR R +A
Sbjct: 202 PQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKSVRHRVLA 258

The following BLAST results are available for this feature:

BLAST of MC09g1103 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XFR9	2.5e-09	23.67	Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ...	[more]
P31237	9.8e-06	23.84	1-aminocyclopropane-1-carboxylate oxidase OS=Actinidia deliciosa OX=3627 GN=ACO ...	[more]
Q7XP65	1.3e-05	24.56	Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
Q9LY48	1.3e-05	23.75	Jasmonate-induced oxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=JOX3 PE=1 SV=1	[more]
Q8S0S6	2.2e-05	23.74	Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]

BLAST of MC09g1103 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146739.1	3.80e-257	100.00	gibberellin 2-beta-dioxygenase 2 [Momordica charantia]	[more]
XP_038892860.1	2.79e-178	73.70	gibberellin 2-beta-dioxygenase 6 [Benincasa hispida]	[more]
XP_011654997.1	4.50e-178	72.25	gibberellin 2-beta-dioxygenase 2 isoform X1 [Cucumis sativus]	[more]
XP_008465218.1	2.36e-176	71.76	PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase [Cucumis melo]	[more]
XP_022943276.1	1.59e-152	67.82	gibberellin 3-beta-dioxygenase 4 [Cucurbita moschata] >KAG6599990.1 Gibberellin ...	[more]

BLAST of MC09g1103 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CYY0	1.84e-257	100.00	gibberellin 2-beta-dioxygenase 2 OS=Momordica charantia OX=3673 GN=LOC111015875 ...	[more]
A0A1S3CNS4	1.14e-176	71.76	1-aminocyclopropane-1-carboxylate oxidase OS=Cucumis melo OX=3656 GN=LOC10350287...	[more]
A0A6J1FRA7	7.69e-153	67.82	gibberellin 3-beta-dioxygenase 4 OS=Cucurbita moschata OX=3662 GN=LOC111448055 P...	[more]
A0A6J1JL54	1.86e-148	67.47	uncharacterized protein LOC111485581 OS=Cucurbita maxima OX=3661 GN=LOC111485581...	[more]
A0A0A0KP52	3.92e-141	80.88	DIOX_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G208470 PE=4...	[more]

BLAST of MC09g1103 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G11150.1	7.8e-75	45.69	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G30040.1	1.8e-10	23.67	gibberellin 2-oxidase	[more]
AT3G55970.1	9.1e-07	23.75	jasmonate-regulated gene 21	[more]
AT4G23340.1	1.2e-06	24.22	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G78440.1	2.6e-06	23.85	Arabidopsis thaliana gibberellin 2-oxidase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 227..354 e-value: 2.1E-7 score: 32.5
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 27..209 e-value: 8.9E-17 score: 63.6
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 64..142 e-value: 7.7E-10 score: 39.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 18..34
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 47..70
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..34
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 52..70
None	No IPR available	PANTHER	PTHR34945	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN	coord: 4..366
None	No IPR available	PANTHER	PTHR34945:SF2	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN	coord: 4..366
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 48..312

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC09g1103.1	MC09g1103.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity