MC09g0827 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g0827
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionSodium Bile acid symporter family
LocationMC09: 8733655 .. 8738442 (+)
RNA-Seq ExpressionMC09g0827
SyntenyMC09g0827
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
TAGCCGTGACAACCCACGAATAAACTCAAACTTTTCGGCCTTGACTCAAGTCTTCATGTCCGCTCTTCTCCACCCGCAACCCAAGTAAAAAAAGAGATTTATCAATACTTATCTAAATTTAAGGTCAAATTTAATGAAAGTAACACTAAAAATCGATACACTTTGTCAAGTTAAAAAATAAACCTAGTTTGGGGTTGAGGGACTAAATCGGTAATTTAAACCATAAGAAAAGAAATTAGACCCAAAGGGAAAAAAGAAAAGAAAAGAATAAACTGAAATCTTCAGAGCTTCAGCTCTTCTCACCCAACACTTGGACAGATACGTCTTTCTTTGTTTTGTTTCCAGTCGTTTTCCTTTTCCCTCCCTTATTTATCGTCTATAAGAACCTCACCGGCGGCGAACCACTCACTTTCCGGCGAATATTCCACCGGCGATTTCACCGTTTATCACTTCATTTTCCTTTCCATGTTCGCCATACCACTATGCTCTTCTCCGGTTCCCTTACTTCCTCAACCGCCTTCCCCGATCAACCATGCTAAATCCAAATTTCATTCATCCCATTGCTCCTACGTCTCCGTTTCCAGTATTTCGAGTTCGCAATCCAGATTGTGGTCTTCGTCTCACTGTTCCACTTCTAAGAATTGCCGGACGGCGAGCAATGGAGCTCTATTTTCGTTTGCTTGTTCGACGTCGCCGTTCGTCGGGAGAGTAGGGTCGCGGATTAGGCAGGGGAATTTCTCGCTCTTGTCTTTTGGATTGAACTCTCATGCGGCGGCGGACGGCGGAGGTGGCGATTCGTCTCAGCTCTTATCGGCGTTGCTTCCTTTTGTGGTAGCTGCAACCGCGATTGCTGCTCTGGCTCGGCCTTCCACTTTTACTTGGTGATTAATTTGGATCTTCTCGATTTGGTTATTGTTGTTTCCAGCTTTCAATGCTCGTTTCTTAGCCTTTTATTTTAATATTTAAAGTGAATTTTATTCTCATTGAAGTCAGCATATTGGACTCTTGTTCTCTGCAAAATGATGATTATTATATTTTTATTCATTCTATAGTTTCTATATCCTTGTTACTAAATGTAGGTTCTGTGGTTCAAGCTTCATTACTATTCATTCACCTGTCAAATATTTAAATAACGGTTTCTTTTATCATCAGCTAGTCAACTTTCTGGCTGTTTTTATCTTATTGTTATATTCATTTTCTATGTTTGTTTCTTTTTTGAGGTGATCTTTGAAACTTTCAATTTGCTGGACTATAATCTGAGAATACAGTCCACAAACTAATATTGAAGTTTCAAATCAGAAGCTTCCCTGTTTTCCCTTTTCAGGTTGCTATCTTATTTAGATGATTGGATCATTGACAAAACTAACATTTATAGTGTTATGTTACCATTTCTGAGGTGGTGCATGTAGTTCATACAAAATATCAAATTAATTTTAGTGTTTTATGAAGTTTTTGACTTGGGATTTTACAGGGTTCCTAAGGATCTCTATGCCCCAGCTCTTGGTGGGATAATGCTATCTATTGGGATTAAGCTTTCCATTGATGATTTTGCTCTAGCAATTAAAAGGTTTATGCATCTTCTGAATTGCCAATCATATTAACCTAACCACATTTGAAAACTCGAGTCTGTTCTTGAGAATTCTTTCATTTTGTCTATTATTTGCAGGCCTCTACCACTTTCTGTTGGGTTTATCACTCAGTATGGTTTGAAACCAGCTCTTGGGATATTGATAGCTAGAGCATGTGGCATATCTCCAATGTTCTATGCAGGATTTCTTCTCACAGCCTGTGTTGGAGGGGCCCAGCTATCAAGTTATGCCAGTTTCTTGAGTAAAGGAGATGTTGCCTTAAGTATTCTCCTTACCAGCTTTACCACCATCTCATCCGTGCTTCTAACACCTCTGTTGACTGGCGTTCTGATCGGTTCTGTTGTTCCAGTTGATGCAGTTGCTATGTCCAAGTCAATATTGCAGGTAATGGTATTTCAATGAATTTTTGTATGATAGATAAGAATCATTGATTTCAAAAATAGTAGTACAATGATAAGGGAAAACAGGGAAACTTTCCATTTTTTTGTAGTATATATCCCATTTATTAATATTCACCCGTGTAATCATTTGGTATATATTATGAGGGGTTTATATTGTTCATTTGTTTTAGTTGGAAAGAGTATTTACTGCAGTAGTACTGGAATTTTTCCCGAGAAACATGTCCTACTTTGTTGCTAATGTCTAAAACTAAGCACTAAAAAAATTAGTACAATGAAGTCTTGATATATTGGATTTCTCATTATCTAGGCACCTGCTTGAATCAGGGAGTTTTTCTAGTCTTCATAGATAAAATTTTCTTCTTGGCAAGTGAGATAAACTAACAGAAACTTCTTATTTGGAGGTTTTATTATGTGTGTTCTTTTCCTTCTCCAGTTCTTCAGTTATTTTTTGCTCTGTTATTACGCATATCCTTCCTATTTCTCAGCTGTAACCAAGCTTTAACAACCTCTCACTAGCAAGAACTGTTCCTCATATTAAAAAGAAAAAAAAAAGAACTGGTATATTTTAAAGGATCAAAGTTGTCCTGTATTATACTGATATAATTATTAATGTTTGGTTATTTACATTCCCGGCATTGATGCAGGTTGTTCTTCTTCCAGTTACTCTTGGACTCGTCCTTAATTCTTATGCAAAGCCAATCGTGGCCATTATTCAGCCCGTGATGCCTTTCGTTGCAATGATATGTACTTCATTGTGCATTGGCAGCCCTCTTGCTATAAACAGGAGCCAAATCTTGTCGATAGACGGTGTCCGGTTGGTTTTTCCAGTATTGGCATTTCATACTTTGGCTTTCACTTTCGGATACTGGGCTTCCAAAATTCCAATTATAAGGTAAAGTTCCACCCCTTTACATAAAGTCTCATACATTTTGACGAATTTTGTGGCTTCAGTTCCTTCAAAATGATCCTTTGGTAGTTGAATTCTTGATTATATAATTGAGTCTATCTGCTGTATATTGTGCATGAAAATAGAAATAGTAGTAACCGTCCATCTATAAAAGTTTGGGAAATTACATGAGTCTATCAGGGCAGCTACATCGGATTCTGATCTTTTGCTTTGATCAATCAATCACCTTCAATCTTTTAGAAAACTGAGATATAGGCATGTGACTATAATTAGTGAAGGCCTTTCAACTCTTCATTCACATGGAAACAATGTGCTGTCATAATGTTGCTTCTTTCTCTGCTCAAGTGGGTCTTAGATTACACTGCTGGCAATAGAGCGTGATATAGAGGGCAGCAGTCAAATCTGTACTTTGTATGGCTTGTTGGGAGCAGTTTATAAAAATTATTTTCGATCTTAAAAATTAGAAATAGAAGGGTTGTTTGCTAAAAAATAAATAAATAAATAAATAGTGAAAACATGTGTGGTAGAATCATAGGAAATTGTTTACTCTTGTTTTTTGTTTTACTTTAATCCCAAAAGTACAAATTTTTATATTAGTATGTTCGGCGGGGGCTCCCTTGGTATAAGGGAGCAAAGCTCCGACTCCCAGTTATACAAAAAGTGCACCCTATAACCTTTTAAGAGCCACCCAAAAGCTATTTTCAAAAGAAAATTTGAAAACGGAAGTAGGAAACTATTTTTCAAAATGGCTTCTGATCAACAAAATTTTAATTTACAAATAAGTGTGTTATTGTGTACTGTTTTTAAGTAATCCTTTTTCTGTTATCTAAAGATAGTTTTCATATGCATTGTTGTTCTTCATATATAACAAAAACTATTTAACTGAAATACAATAGCAAAGAAGAATCTGTTCAAACCATGTTTCGAAGTGAAAAATAACTAACAACTTTTCAAAATTGTTTAGAACGCATTTAATATATGATTTTACAAAAAAACTACTTCCATATAGACTGCCAAAGACGTTTTCTCAAATTTCTGCATTAATGTTGTAAGCTTTCTTTCTAATTCATGCCTTTGATATACATTGTTTAGAAGAGAGTCATGCCACTTTGTTTATTAGCCTGGTTTGGATTGAATTCACATTAGATTGACCCCGAAGCGGCAACCCCGTTGTCTCCCGACTCGGGGTCCTACTTCCTGATTTTTCGAACCTTCCAGTAGAAAGTTTAATAAATGAAAAACTTCGATAACTTTTCAGAGCTTAGCATTAGATTGAATACTAGATTTCGAGACATGTTGCACATTGCTTGATTGCCATTTGTTATAAATCTGAAACTGCGGGTCAATGTCGTCGTTGGCGCAGGCAAGAAGAAGAAGCTAGCAGAACCATTTCACTGTGCACAGGGATGCAGAGCTCCACCCTAGCAGGGCTTCTCGCTACTCAGTTCCTCGGGAGCACGCAGGCCGTTCCACCAGCGTGCTCGGTAGTCGCCATGGCGATCATGGGCCTTTGTCTAGCTTCCTTCTGGGGCAGTGGCTCAAAAATCAGAGACTTGCCATCTCTTTTGATACAGAAAACCAATTAATTCCATTGCCAGTGCTTCTTGAATCTGCTTGAATTAGCAACCTCTCCATCTCTTAAAACTGATTGGAAGCCCCACAAGGGAAAAAAAGTGCTTCTGCCAATAGCAGCAGCAGCAGTTGGAAACTTTTCTGTACAGAGAGAAATCAGGTACCACATAGAATACTAAGATAGCAGGAAATATATATCTTTACTTTTATAAGTTCATATGATACAATAAATTTCCTGGATGCTGGATAATCCACAAGGTATAAAGGTATCATAATAGTTTTCAAATAAAAGCAAGAAAAAAAAAATGTTTTGGCATCA

mRNA sequence

TAGCCGTGACAACCCACGAATAAACTCAAACTTTTCGGCCTTGACTCAAGTCTTCATGTCCGCTCTTCTCCACCCGCAACCCAAGTAAAAAAAGAGATTTATCAATACTTATCTAAATTTAAGGTCAAATTTAATGAAAGTAACACTAAAAATCGATACACTTTGTCAAGTTAAAAAATAAACCTAGTTTGGGGTTGAGGGACTAAATCGGTAATTTAAACCATAAGAAAAGAAATTAGACCCAAAGGGAAAAAAGAAAAGAAAAGAATAAACTGAAATCTTCAGAGCTTCAGCTCTTCTCACCCAACACTTGGACAGATACGTCTTTCTTTGTTTTGTTTCCAGTCGTTTTCCTTTTCCCTCCCTTATTTATCGTCTATAAGAACCTCACCGGCGGCGAACCACTCACTTTCCGGCGAATATTCCACCGGCGATTTCACCGTTTATCACTTCATTTTCCTTTCCATGTTCGCCATACCACTATGCTCTTCTCCGGTTCCCTTACTTCCTCAACCGCCTTCCCCGATCAACCATGCTAAATCCAAATTTCATTCATCCCATTGCTCCTACGTCTCCGTTTCCAGTATTTCGAGTTCGCAATCCAGATTGTGGTCTTCGTCTCACTGTTCCACTTCTAAGAATTGCCGGACGGCGAGCAATGGAGCTCTATTTTCGTTTGCTTGTTCGACGTCGCCGTTCGTCGGGAGAGTAGGGTCGCGGATTAGGCAGGGGAATTTCTCGCTCTTGTCTTTTGGATTGAACTCTCATGCGGCGGCGGACGGCGGAGGTGGCGATTCGTCTCAGCTCTTATCGGCGTTGCTTCCTTTTGTGGTAGCTGCAACCGCGATTGCTGCTCTGGCTCGGCCTTCCACTTTTACTTGGGTTCCTAAGGATCTCTATGCCCCAGCTCTTGGTGGGATAATGCTATCTATTGGGATTAAGCTTTCCATTGATGATTTTGCTCTAGCAATTAAAAGGCCTCTACCACTTTCTGTTGGGTTTATCACTCAGTATGGTTTGAAACCAGCTCTTGGGATATTGATAGCTAGAGCATGTGGCATATCTCCAATGTTCTATGCAGGATTTCTTCTCACAGCCTGTGTTGGAGGGGCCCAGCTATCAAGTTATGCCAGTTTCTTGAGTAAAGGAGATGTTGCCTTAAGTATTCTCCTTACCAGCTTTACCACCATCTCATCCGTGCTTCTAACACCTCTGTTGACTGGCGTTCTGATCGGTTCTGTTGTTCCAGTTGATGCAGTTGCTATGTCCAAGTCAATATTGCAGGTTGTTCTTCTTCCAGTTACTCTTGGACTCGTCCTTAATTCTTATGCAAAGCCAATCGTGGCCATTATTCAGCCCGTGATGCCTTTCGTTGCAATGATATGTACTTCATTGTGCATTGGCAGCCCTCTTGCTATAAACAGGAGCCAAATCTTGTCGATAGACGGTGTCCGGTTGGTTTTTCCAGTATTGGCATTTCATACTTTGGCTTTCACTTTCGGATACTGGGCTTCCAAAATTCCAATTATAAGGCAAGAAGAAGAAGCTAGCAGAACCATTTCACTGTGCACAGGGATGCAGAGCTCCACCCTAGCAGGGCTTCTCGCTACTCAGTTCCTCGGGAGCACGCAGGCCGTTCCACCAGCGTGCTCGGTAGTCGCCATGGCGATCATGGGCCTTTGTCTAGCTTCCTTCTGGGGCAGTGGCTCAAAAATCAGAGACTTGCCATCTCTTTTGATACAGAAAACCAATTAATTCCATTGCCAGTGCTTCTTGAATCTGCTTGAATTAGCAACCTCTCCATCTCTTAAAACTGATTGGAAGCCCCACAAGGGAAAAAAAGTGCTTCTGCCAATAGCAGCAGCAGCAGTTGGAAACTTTTCTGTACAGAGAGAAATCAGGTACCACATAGAATACTAAGATAGCAGGAAATATATATCTTTACTTTTATAAGTTCATATGATACAATAAATTTCCTGGATGCTGGATAATCCACAAGGTATAAAGGTATCATAATAGTTTTCAAATAAAAGCAAGAAAAAAAAAATGTTTTGGCATCA

Coding sequence (CDS)

ATGTTCGCCATACCACTATGCTCTTCTCCGGTTCCCTTACTTCCTCAACCGCCTTCCCCGATCAACCATGCTAAATCCAAATTTCATTCATCCCATTGCTCCTACGTCTCCGTTTCCAGTATTTCGAGTTCGCAATCCAGATTGTGGTCTTCGTCTCACTGTTCCACTTCTAAGAATTGCCGGACGGCGAGCAATGGAGCTCTATTTTCGTTTGCTTGTTCGACGTCGCCGTTCGTCGGGAGAGTAGGGTCGCGGATTAGGCAGGGGAATTTCTCGCTCTTGTCTTTTGGATTGAACTCTCATGCGGCGGCGGACGGCGGAGGTGGCGATTCGTCTCAGCTCTTATCGGCGTTGCTTCCTTTTGTGGTAGCTGCAACCGCGATTGCTGCTCTGGCTCGGCCTTCCACTTTTACTTGGGTTCCTAAGGATCTCTATGCCCCAGCTCTTGGTGGGATAATGCTATCTATTGGGATTAAGCTTTCCATTGATGATTTTGCTCTAGCAATTAAAAGGCCTCTACCACTTTCTGTTGGGTTTATCACTCAGTATGGTTTGAAACCAGCTCTTGGGATATTGATAGCTAGAGCATGTGGCATATCTCCAATGTTCTATGCAGGATTTCTTCTCACAGCCTGTGTTGGAGGGGCCCAGCTATCAAGTTATGCCAGTTTCTTGAGTAAAGGAGATGTTGCCTTAAGTATTCTCCTTACCAGCTTTACCACCATCTCATCCGTGCTTCTAACACCTCTGTTGACTGGCGTTCTGATCGGTTCTGTTGTTCCAGTTGATGCAGTTGCTATGTCCAAGTCAATATTGCAGGTTGTTCTTCTTCCAGTTACTCTTGGACTCGTCCTTAATTCTTATGCAAAGCCAATCGTGGCCATTATTCAGCCCGTGATGCCTTTCGTTGCAATGATATGTACTTCATTGTGCATTGGCAGCCCTCTTGCTATAAACAGGAGCCAAATCTTGTCGATAGACGGTGTCCGGTTGGTTTTTCCAGTATTGGCATTTCATACTTTGGCTTTCACTTTCGGATACTGGGCTTCCAAAATTCCAATTATAAGGCAAGAAGAAGAAGCTAGCAGAACCATTTCACTGTGCACAGGGATGCAGAGCTCCACCCTAGCAGGGCTTCTCGCTACTCAGTTCCTCGGGAGCACGCAGGCCGTTCCACCAGCGTGCTCGGTAGTCGCCATGGCGATCATGGGCCTTTGTCTAGCTTCCTTCTGGGGCAGTGGCTCAAAAATCAGAGACTTGCCATCTCTTTTGATACAGAAAACCAATTAA

Protein sequence

MFAIPLCSSPVPLLPQPPSPINHAKSKFHSSHCSYVSVSSISSSQSRLWSSSHCSTSKNCRTASNGALFSFACSTSPFVGRVGSRIRQGNFSLLSFGLNSHAAADGGGGDSSQLLSALLPFVVAATAIAALARPSTFTWVPKDLYAPALGGIMLSIGIKLSIDDFALAIKRPLPLSVGFITQYGLKPALGILIARACGISPMFYAGFLLTACVGGAQLSSYASFLSKGDVALSILLTSFTTISSVLLTPLLTGVLIGSVVPVDAVAMSKSILQVVLLPVTLGLVLNSYAKPIVAIIQPVMPFVAMICTSLCIGSPLAINRSQILSIDGVRLVFPVLAFHTLAFTFGYWASKIPIIRQEEEASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDLPSLLIQKTN
Homology
BLAST of MC09g0827 vs. ExPASy Swiss-Prot
Match: Q8RXE8 (Probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BASS3 PE=2 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 1.8e-136
Identity = 275/412 (66.75%), Postives = 323/412 (78.40%), Query Frame = 0

Query: 17  PPSPINHAKSKFHSSHCSYVSVSSISSSQSRLWSSSHCSTSKN-----CRTASNGALFSF 76
           PP+    + S+FH+      S +    S S   SS H S+  N      R  ++G +   
Sbjct: 11  PPAIQRQSLSEFHNLPAKSQSFNCQFRSSSSPLSSLHSSSLSNFLDFRLRRRNSGLVPVV 70

Query: 77  ACSTSPFVGRVGSRIRQGNFSLLSFGLNSHAAADGGGGDSSQLLSALLPFVVAATAIAAL 136
           ACST+PF+GRVG   R GN SLLSF   +         DSSQ  SALLPFVVA TA+AAL
Sbjct: 71  ACSTTPFMGRVGLHWRDGNMSLLSFCGGTDVTEK---ADSSQFWSALLPFVVALTAVAAL 130

Query: 137 ARPSTFTWVPKDLYAPALGGIMLSIGIKLSIDDFALAIKRPLPLSVGFITQYGLKPALGI 196
           + P +FTWV KDLYAPALGGIMLSIGI+LS+DDFALA KRP+PLSVGF+ QY LKP LG+
Sbjct: 131 SYPPSFTWVSKDLYAPALGGIMLSIGIQLSVDDFALAFKRPVPLSVGFVAQYVLKPLLGV 190

Query: 197 LIARACGISPMFYAGFLLTACVGGAQLSSYASFLSKGDVALSILLTSFTTISSVLLTPLL 256
           L+A A G+   FYAGF+LT CV GAQLSSYAS LSK DVA+SILLTS TTI+SV+ TPLL
Sbjct: 191 LVANAFGMPRTFYAGFILTCCVAGAQLSSYASSLSKADVAMSILLTSSTTIASVIFTPLL 250

Query: 257 TGVLIGSVVPVDAVAMSKSILQVVLLPVTLGLVLNSYAKPIVAIIQPVMPFVAMICTSLC 316
           +G+LIGSVVPVDAVAMSKSILQVVL+P+TLGLVLN+YAKP+V ++QPVMPFVAM+CTSLC
Sbjct: 251 SGLLIGSVVPVDAVAMSKSILQVVLVPITLGLVLNTYAKPVVTLLQPVMPFVAMVCTSLC 310

Query: 317 IGSPLAINRSQILSIDGVRLVFPVLAFHTLAFTFGYWASKIPIIRQEEEASRTISLCTGM 376
           IGSPL+INRSQILS +G+ L+ P++ FH +AF  GYW SKIP +RQEEE SRTISLCTGM
Sbjct: 311 IGSPLSINRSQILSAEGLGLIVPIVTFHAVAFALGYWFSKIPGLRQEEEVSRTISLCTGM 370

Query: 377 QSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDLPSL 424
           QSSTLAGLLA+QFLGS+QAVP ACSVV MAIMGLCLASFWG+G +IRD+ SL
Sbjct: 371 QSSTLAGLLASQFLGSSQAVPAACSVVVMAIMGLCLASFWGNGFRIRDVLSL 419

BLAST of MC09g0827 vs. ExPASy Swiss-Prot
Match: Q6K739 (Probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BASS3 PE=2 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 1.0e-123
Identity = 260/414 (62.80%), Postives = 315/414 (76.09%), Query Frame = 0

Query: 24  AKSKFHSSHCSYVSVSSIS----SSQSRLWSSSHCSTSKNC---RTASNGALFSFACSTS 83
           A S   SS C+ V V++ S      Q+R   S     S      R     A  +F CS +
Sbjct: 7   ASSSSSSSSCAAVGVATASHPHRHRQARFVVSPPAPASPAALLWRRPRRVAPTTF-CS-A 66

Query: 84  PFVGRVGSRIRQGNFSLLSFGLNS-HAAADGGGGDSSQLLSALLPFVVAATAIAALARPS 143
           P +GRVG   R+G   LLSF      + +    GD SQ LSALLP VVAATA+AAL  P+
Sbjct: 67  PSLGRVGWPRREGAAWLLSFRAGPVSSPSSAAAGDPSQALSALLPLVVAATAVAALGNPA 126

Query: 144 TFTWVPKDLYAPALGGIMLSIGIKLSIDDFALAIKRPLPLSVGFITQYGLKPALGILIAR 203
           TF+WV K+ YAPALGGIMLSIGIKLSIDDFALA KRP+PL++G++ QY +KP +G+LIAR
Sbjct: 127 TFSWVSKEYYAPALGGIMLSIGIKLSIDDFALAFKRPVPLTIGYMAQYIVKPLMGVLIAR 186

Query: 204 ACGISPMFYAGFLLTACVGGAQLSSYASFLSKGDVALSILLTSFTTISSVLLTPLLTGVL 263
           A G+   F+AGF+LT CV GAQLSSYASFLSKGDVALSILLTS +TISSV++TP+LTG+L
Sbjct: 187 AFGMPSAFFAGFVLTCCVSGAQLSSYASFLSKGDVALSILLTSCSTISSVVVTPVLTGLL 246

Query: 264 IGSVVPVDAVAMSKSILQVVLLPVTLGLVLNSYAKPIVAIIQPVMPFVAMICTSLCIGSP 323
           IGSVVPVD +AM+KSILQVVL+PVTLGL+LN+YAK +V +IQPVMPFVAM+CTSLCIGSP
Sbjct: 247 IGSVVPVDGIAMAKSILQVVLVPVTLGLLLNTYAKAVVNVIQPVMPFVAMLCTSLCIGSP 306

Query: 324 LAINRSQILSIDGVRLVFPVLAFHTLAFTFGYWASKIPIIRQEEEASRTISLCTGMQSST 383
           LAINRS+ILS +G  L+ P++ FH  AF  GYW SK+P++RQEE   RTIS+CTGMQSST
Sbjct: 307 LAINRSKILSSEGFLLLLPIVTFHIAAFIVGYWISKLPMLRQEEPVCRTISVCTGMQSST 366

Query: 384 LAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDLPSLLIQKTN 430
           LAGLLATQFLGS+QAVP ACSVV MAI GL LAS+WG+G +IRD+ S  + + +
Sbjct: 367 LAGLLATQFLGSSQAVPAACSVVIMAIFGLTLASYWGNGLRIRDIGSRFVPQAS 418

BLAST of MC09g0827 vs. ExPASy Swiss-Prot
Match: Q1EBV7 (Sodium/pyruvate cotransporter BASS2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BASS2 PE=1 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 6.9e-51
Identity = 123/300 (41.00%), Postives = 177/300 (59.00%), Query Frame = 0

Query: 113 QLLSALLPFVVAATAIAALARPSTFTWVPKDLYAPALGGIMLSIGIKLSIDDFALAIKRP 172
           +LL+ L P  V    +  + +PS  TW+  DL+   LG +MLS+G+ L+ +DF   ++ P
Sbjct: 99  ELLTTLFPLWVILGTLVGIFKPSLVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNP 158

Query: 173 LPLSVGFITQYGLKPALGILIARACGISPMFYAGFLLTACVGGAQLSSYASFLSKGDVAL 232
             + VGF+ QY +KP LG LIA    +S     G +L +C  G Q S+ A+++SKG+VAL
Sbjct: 159 WTVGVGFLAQYMIKPILGFLIAMTLKLSAPLATGLILVSCCPGGQASNVATYISKGNVAL 218

Query: 233 SILLTSFTTISSVLLTPLLTGVLIGSVVPVDAVAMSKSILQVVLLPVTLGLVLNSYAKPI 292
           S+L+T+ +TI ++++TPLLT +L G +VPVDA  ++ S  QVVL+P  +G++ N +    
Sbjct: 219 SVLMTTCSTIGAIIMTPLLTKLLAGQLVPVDAAGLALSTFQVVLVPTIIGVLANEFFPKF 278

Query: 293 VAIIQPVMPFVAMICTSLCIGSPLAINRSQILSIDGVRLVFPVLAFHTLAFTFGYWASKI 352
            + I  V P + +I T+L   SP+    + +L   G +L+ PV   H  AF  GYW SK 
Sbjct: 279 TSKIITVTPLIGVILTTLLCASPIG-QVADVLKTQGAQLILPVALLHAAAFAIGYWISKF 338

Query: 353 PIIRQEEEASRTISLCTGMQSSTLAGLLATQ-FLGSTQAVPPACSVVAMAIMGLCLASFW 412
                 E  SRTIS+  GMQSS L  LLA + F     AVP A SVV MA+ G  LA FW
Sbjct: 339 SF---GESTSRTISIECGMQSSALGFLLAQKHFTNPLVAVPSAVSVVCMALGGSGLAVFW 394

BLAST of MC09g0827 vs. ExPASy Swiss-Prot
Match: Q5VRB2 (Probable sodium/metabolite cotransporter BASS2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BASS2 PE=2 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 2.6e-50
Identity = 123/300 (41.00%), Postives = 177/300 (59.00%), Query Frame = 0

Query: 113 QLLSALLPFVVAATAIAALARPSTFTWVPKDLYAPALGGIMLSIGIKLSIDDFALAIKRP 172
           +LL+ L P  V    I  + +PS  TW+  DL+   LG +MLS+G+ L+ +DF   ++ P
Sbjct: 108 ELLTTLFPVWVILGTIIGIYKPSMVTWLETDLFTVGLGFLMLSMGLTLTFEDFRRCMRNP 167

Query: 173 LPLSVGFITQYGLKPALGILIARACGISPMFYAGFLLTACVGGAQLSSYASFLSKGDVAL 232
             + VGF+ QY +KP LG  IA    +S     G +L +C  G Q S+ A+++SKG+VAL
Sbjct: 168 WTVGVGFLAQYLIKPMLGFAIAMTLKLSAPLATGLILVSCCPGGQASNVATYISKGNVAL 227

Query: 233 SILLTSFTTISSVLLTPLLTGVLIGSVVPVDAVAMSKSILQVVLLPVTLGLVLNSYAKPI 292
           S+L+T+ +TI ++++TPLLT +L G +VPVDA  ++ S  QVVLLP  +G++ + Y    
Sbjct: 228 SVLMTTCSTIGAIVMTPLLTKLLAGQLVPVDAAGLAISTFQVVLLPTIVGVLAHEYFPKF 287

Query: 293 VAIIQPVMPFVAMICTSLCIGSPLAINRSQILSIDGVRLVFPVLAFHTLAFTFGYWASKI 352
              I  + P + ++ T+L   SP+    S++L   G +L+ PV   H  AF  GYW SK+
Sbjct: 288 TERIISITPLIGVLLTTLLCASPIG-QVSEVLKAQGGQLIIPVALLHVAAFALGYWLSKV 347

Query: 353 PIIRQEEEASRTISLCTGMQSSTLAGLLATQ-FLGSTQAVPPACSVVAMAIMGLCLASFW 412
                 E  SRTIS+  GMQSS L  LLA + F     AVP A SVV MA+ G  LA FW
Sbjct: 348 SSF--GESTSRTISIECGMQSSALGFLLAQKHFTNPLVAVPSAVSVVCMALGGSALAVFW 404

BLAST of MC09g0827 vs. ExPASy Swiss-Prot
Match: B8BDK4 (Probable sodium/metabolite cotransporter BASS5, chloroplastic OS=Oryza sativa subsp. indica OX=39946 GN=BASS5 PE=3 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 1.7e-49
Identity = 127/312 (40.71%), Postives = 184/312 (58.97%), Query Frame = 0

Query: 116 SALLPFVVAATAIAALARPSTFTWVPKDLYAPALGGIMLSIGIKLSIDDFALAIKRPLPL 175
           + ++P VV  + I AL  P +FTW     YAPALG +M ++G+  S+ DF  AI+RP  +
Sbjct: 92  NTIIPHVVLGSTILALVYPPSFTWFTTRYYAPALGFLMFAVGVNSSVKDFIEAIQRPDAI 151

Query: 176 SVGFITQYGLKPALGILIARAC----GISPMFYAGFLLTACVGGAQLSSYASFLSKGDVA 235
           + G++ Q+ +KP LG L          +     AG +L +CV GAQLS+YA+FL+   +A
Sbjct: 152 AAGYVGQFIIKPFLGFLFGTLAVTIFNLPTALGAGIMLVSCVSGAQLSNYATFLTDPHMA 211

Query: 236 -LSILLTSFTTISSVLLTPLLTGVLIGSVVPVDAVAMSKSILQVVLLPVTLGLVLNSYAK 295
            LSI++TS +T ++V +TP L+  LIG  +PVD   M  SI+Q+V+ P+  GL+LN Y  
Sbjct: 212 PLSIVMTSLSTATAVFVTPTLSYFLIGKKLPVDVKGMMSSIVQIVVAPIAAGLLLNRYLP 271

Query: 296 PIVAIIQPVMPFVAMICTSLCIGSPLAINRSQILSIDGVRLVFPVLAFHTLAFTFGY--- 355
            + + IQP +P +++  T+LC+GSPLAIN   +LS  G+  V  + AFHT +F  GY   
Sbjct: 272 RLCSAIQPFLPPLSVFVTALCVGSPLAINIKAVLSPFGLATVLLLFAFHTSSFIAGYHLA 331

Query: 356 --WASKIPIIRQEEEASRTISLCTGMQSSTLAGLLATQFL-GSTQAVPPACSVVAMAIMG 415
             W  +   ++      RT+S  TGMQSS LA  LA +F       VPPA SVV M++MG
Sbjct: 332 GTWFRESADVK---ALQRTVSFETGMQSSLLALALANRFFPDPLVGVPPAISVVLMSLMG 391

Query: 416 LCLASFWGSGSK 417
             L   W   +K
Sbjct: 392 FALVMVWSKRTK 400

BLAST of MC09g0827 vs. NCBI nr
Match: XP_022156093.1 (probable sodium/metabolite cotransporter BASS3, chloroplastic [Momordica charantia])

HSP 1 Score: 801 bits (2068), Expect = 1.94e-291
Identity = 429/429 (100.00%), Postives = 429/429 (100.00%), Query Frame = 0

Query: 1   MFAIPLCSSPVPLLPQPPSPINHAKSKFHSSHCSYVSVSSISSSQSRLWSSSHCSTSKNC 60
           MFAIPLCSSPVPLLPQPPSPINHAKSKFHSSHCSYVSVSSISSSQSRLWSSSHCSTSKNC
Sbjct: 1   MFAIPLCSSPVPLLPQPPSPINHAKSKFHSSHCSYVSVSSISSSQSRLWSSSHCSTSKNC 60

Query: 61  RTASNGALFSFACSTSPFVGRVGSRIRQGNFSLLSFGLNSHAAADGGGGDSSQLLSALLP 120
           RTASNGALFSFACSTSPFVGRVGSRIRQGNFSLLSFGLNSHAAADGGGGDSSQLLSALLP
Sbjct: 61  RTASNGALFSFACSTSPFVGRVGSRIRQGNFSLLSFGLNSHAAADGGGGDSSQLLSALLP 120

Query: 121 FVVAATAIAALARPSTFTWVPKDLYAPALGGIMLSIGIKLSIDDFALAIKRPLPLSVGFI 180
           FVVAATAIAALARPSTFTWVPKDLYAPALGGIMLSIGIKLSIDDFALAIKRPLPLSVGFI
Sbjct: 121 FVVAATAIAALARPSTFTWVPKDLYAPALGGIMLSIGIKLSIDDFALAIKRPLPLSVGFI 180

Query: 181 TQYGLKPALGILIARACGISPMFYAGFLLTACVGGAQLSSYASFLSKGDVALSILLTSFT 240
           TQYGLKPALGILIARACGISPMFYAGFLLTACVGGAQLSSYASFLSKGDVALSILLTSFT
Sbjct: 181 TQYGLKPALGILIARACGISPMFYAGFLLTACVGGAQLSSYASFLSKGDVALSILLTSFT 240

Query: 241 TISSVLLTPLLTGVLIGSVVPVDAVAMSKSILQVVLLPVTLGLVLNSYAKPIVAIIQPVM 300
           TISSVLLTPLLTGVLIGSVVPVDAVAMSKSILQVVLLPVTLGLVLNSYAKPIVAIIQPVM
Sbjct: 241 TISSVLLTPLLTGVLIGSVVPVDAVAMSKSILQVVLLPVTLGLVLNSYAKPIVAIIQPVM 300

Query: 301 PFVAMICTSLCIGSPLAINRSQILSIDGVRLVFPVLAFHTLAFTFGYWASKIPIIRQEEE 360
           PFVAMICTSLCIGSPLAINRSQILSIDGVRLVFPVLAFHTLAFTFGYWASKIPIIRQEEE
Sbjct: 301 PFVAMICTSLCIGSPLAINRSQILSIDGVRLVFPVLAFHTLAFTFGYWASKIPIIRQEEE 360

Query: 361 ASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDL 420
           ASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDL
Sbjct: 361 ASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDL 420

Query: 421 PSLLIQKTN 429
           PSLLIQKTN
Sbjct: 421 PSLLIQKTN 429

BLAST of MC09g0827 vs. NCBI nr
Match: XP_038899090.1 (probable sodium/metabolite cotransporter BASS3, chloroplastic [Benincasa hispida])

HSP 1 Score: 670 bits (1729), Expect = 1.08e-238
Identity = 369/429 (86.01%), Postives = 390/429 (90.91%), Query Frame = 0

Query: 1   MFAIPLCSSPVPLLPQPPSPINHAKSKFHSSHCSYVSVSSISSSQSRLWSSSHCSTSKNC 60
           M AI L SSPVP L Q P+  +  K K +SS C  +S+S    S +RLW SSHCSTSKN 
Sbjct: 69  MSAITLYSSPVPFLSQSPTKPSR-KPKLYSSDCHCLSISC---SFTRLWPSSHCSTSKNW 128

Query: 61  RTASNGALFSFACSTSPFVGRVGSRIRQGNFSLLSFGLNSHAAADGGGGDSSQLLSALLP 120
           +   NGAL +FACSTSPF+GRVGSRIR GNFSLLSFGLNS+AAA+  GGDSSQLLSALLP
Sbjct: 129 KPVCNGALSTFACSTSPFIGRVGSRIRLGNFSLLSFGLNSNAAAEAAGGDSSQLLSALLP 188

Query: 121 FVVAATAIAALARPSTFTWVPKDLYAPALGGIMLSIGIKLSIDDFALAIKRPLPLSVGFI 180
           FVV AT IAALARPSTFTWVPKDLYAPALGGIMLSIGIKLSI+DFALAIKRP+PLSVGFI
Sbjct: 189 FVVLATGIAALARPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAIKRPVPLSVGFI 248

Query: 181 TQYGLKPALGILIARACGISPMFYAGFLLTACVGGAQLSSYASFLSKGDVALSILLTSFT 240
            QY LKPALG+LIARA GISPMFYAGFLLTACVGGAQLSSYASFLSKGD+ALSILLTS T
Sbjct: 249 AQYVLKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSILLTSIT 308

Query: 241 TISSVLLTPLLTGVLIGSVVPVDAVAMSKSILQVVLLPVTLGLVLNSYAKPIVAIIQPVM 300
           TISSVLLTPLLTG+LIGSVVPVDAVAMSKSILQVVLLPV LGLVLNSYAKPIVA++QPVM
Sbjct: 309 TISSVLLTPLLTGILIGSVVPVDAVAMSKSILQVVLLPVALGLVLNSYAKPIVAVLQPVM 368

Query: 301 PFVAMICTSLCIGSPLAINRSQILSIDGVRLVFPVLAFHTLAFTFGYWASKIPIIRQEEE 360
           PFVAMICTSLCIGSPLAINRSQILSIDG RLV PVLAFHTLAFT GYWASKIPI+RQEEE
Sbjct: 369 PFVAMICTSLCIGSPLAINRSQILSIDGARLVLPVLAFHTLAFTLGYWASKIPIVRQEEE 428

Query: 361 ASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDL 420
           ASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDL
Sbjct: 429 ASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDL 488

Query: 421 PSLLIQKTN 429
           PSLLIQK++
Sbjct: 489 PSLLIQKSS 493

BLAST of MC09g0827 vs. NCBI nr
Match: XP_022991663.1 (probable sodium/metabolite cotransporter BASS3, chloroplastic [Cucurbita maxima])

HSP 1 Score: 657 bits (1695), Expect = 1.30e-234
Identity = 363/429 (84.62%), Postives = 387/429 (90.21%), Query Frame = 0

Query: 1   MFAIPLCSSPVPLLPQPPSPINHAKSKFHSSHCSYVSVSSISSSQSRLWSSSHCSTSKNC 60
           M AI LCSSPVP   Q P      K K +SS C  +S S    + +RLWSS +CSTSKN 
Sbjct: 1   MSAIALCSSPVPFPSQLPIKPRR-KPKLYSSDCLCLSFSC---NLTRLWSS-YCSTSKNW 60

Query: 61  RTASNGALFSFACSTSPFVGRVGSRIRQGNFSLLSFGLNSHAAADGGGGDSSQLLSALLP 120
            T  NGA+ +FACSTSPFVGR+GSRIRQGNFSLLSFGLNS+A A+   GDSSQLLSALLP
Sbjct: 61  STVRNGAVSTFACSTSPFVGRIGSRIRQGNFSLLSFGLNSNAEAEATDGDSSQLLSALLP 120

Query: 121 FVVAATAIAALARPSTFTWVPKDLYAPALGGIMLSIGIKLSIDDFALAIKRPLPLSVGFI 180
           FVVAATAIAAL RPSTFTWV KD YAPALGGIMLSIGIKLSI+DFALAIKRPLPLSVGFI
Sbjct: 121 FVVAATAIAALTRPSTFTWVSKDFYAPALGGIMLSIGIKLSINDFALAIKRPLPLSVGFI 180

Query: 181 TQYGLKPALGILIARACGISPMFYAGFLLTACVGGAQLSSYASFLSKGDVALSILLTSFT 240
            QY LKPALG+LIARA GISPMFYAGFLLTACVGGAQLSSYASFLSKGD+ALS+LLTSFT
Sbjct: 181 AQYVLKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSVLLTSFT 240

Query: 241 TISSVLLTPLLTGVLIGSVVPVDAVAMSKSILQVVLLPVTLGLVLNSYAKPIVAIIQPVM 300
           TISSVLLTPLLTG+LIGSVVPVDAVAMSKSILQVVL+P++LGLVLNSYAKPIVA++QPVM
Sbjct: 241 TISSVLLTPLLTGILIGSVVPVDAVAMSKSILQVVLVPISLGLVLNSYAKPIVALLQPVM 300

Query: 301 PFVAMICTSLCIGSPLAINRSQILSIDGVRLVFPVLAFHTLAFTFGYWASKIPIIRQEEE 360
           PFVAMICTSLCIGSPLAINRSQILSIDG RLVFPVL FHT+AFT GYWASKIPI+RQEEE
Sbjct: 301 PFVAMICTSLCIGSPLAINRSQILSIDGARLVFPVLVFHTMAFTLGYWASKIPIVRQEEE 360

Query: 361 ASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDL 420
           ASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDL
Sbjct: 361 ASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDL 420

Query: 421 PSLLIQKTN 429
           PSLL+QKT+
Sbjct: 421 PSLLMQKTS 424

BLAST of MC09g0827 vs. NCBI nr
Match: XP_023549213.1 (probable sodium/metabolite cotransporter BASS3, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 656 bits (1693), Expect = 2.62e-234
Identity = 363/429 (84.62%), Postives = 387/429 (90.21%), Query Frame = 0

Query: 1   MFAIPLCSSPVPLLPQPPSPINHAKSKFHSSHCSYVSVSSISSSQSRLWSSSHCSTSKNC 60
           M AI LCSSPVP   Q P      K K +SS C  +S S    + +RLWSS HCSTSKN 
Sbjct: 1   MSAIALCSSPVPFPSQLPIKPRR-KPKLYSSDCLCLSFSC---NLTRLWSS-HCSTSKNW 60

Query: 61  RTASNGALFSFACSTSPFVGRVGSRIRQGNFSLLSFGLNSHAAADGGGGDSSQLLSALLP 120
            T  NGA+ +FACSTS FVGR+GSRIRQGNFSLLSFGLNS+A A+   GDSSQLLSALLP
Sbjct: 61  STVRNGAVSTFACSTSSFVGRIGSRIRQGNFSLLSFGLNSNAEAEATDGDSSQLLSALLP 120

Query: 121 FVVAATAIAALARPSTFTWVPKDLYAPALGGIMLSIGIKLSIDDFALAIKRPLPLSVGFI 180
           FVVAATAIAAL RPSTFTWV KD YAPALGGIMLSIGIKLSI+DFALAIKRPLPLSVGFI
Sbjct: 121 FVVAATAIAALTRPSTFTWVSKDFYAPALGGIMLSIGIKLSINDFALAIKRPLPLSVGFI 180

Query: 181 TQYGLKPALGILIARACGISPMFYAGFLLTACVGGAQLSSYASFLSKGDVALSILLTSFT 240
            QY LKPALG+LIARA GISPMFYAGFLLTACVGGAQLSSYASFLSKGD+ALS+LLTSFT
Sbjct: 181 AQYVLKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSVLLTSFT 240

Query: 241 TISSVLLTPLLTGVLIGSVVPVDAVAMSKSILQVVLLPVTLGLVLNSYAKPIVAIIQPVM 300
           TISSVLLTPLLTG+LIGSVVPVDAVAM+KSILQVVL+P++LGLVLNSYAKPIVA++QPVM
Sbjct: 241 TISSVLLTPLLTGILIGSVVPVDAVAMAKSILQVVLVPISLGLVLNSYAKPIVALLQPVM 300

Query: 301 PFVAMICTSLCIGSPLAINRSQILSIDGVRLVFPVLAFHTLAFTFGYWASKIPIIRQEEE 360
           PFVAMICTSLCIGSPLAINRSQILSIDG RLVFPVLAFHT+AFT GYWASKIPI+RQEEE
Sbjct: 301 PFVAMICTSLCIGSPLAINRSQILSIDGARLVFPVLAFHTVAFTLGYWASKIPIVRQEEE 360

Query: 361 ASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDL 420
           ASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDL
Sbjct: 361 ASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDL 420

Query: 421 PSLLIQKTN 429
           PSLL+QKT+
Sbjct: 421 PSLLMQKTS 424

BLAST of MC09g0827 vs. NCBI nr
Match: XP_004150363.1 (probable sodium/metabolite cotransporter BASS3, chloroplastic [Cucumis sativus] >KAE8646275.1 hypothetical protein Csa_016116 [Cucumis sativus])

HSP 1 Score: 656 bits (1692), Expect = 4.15e-234
Identity = 358/431 (83.06%), Postives = 385/431 (89.33%), Query Frame = 0

Query: 1   MFAIPLCSSPVPLLPQPPSPINHAKSKFHSSHCSYVSVSSISSSQSRLWSSSHCSTSKNC 60
           M AI LCSSP+P L   P   +     + S +C  +SVS    S +RLW SSHCSTSKN 
Sbjct: 1   MSAISLCSSPLPFLSHLPIKPSRKPKLYSSDYCHCLSVSC---SLTRLWPSSHCSTSKNW 60

Query: 61  RTASNGALFSFACSTSPFVGRVGSRIRQGNFSLLSFGLNSHAAA--DGGGGDSSQLLSAL 120
           +   NGAL +FACSTSPF+GRVGSRIR GNFSLLSFGLNS+AAA  +G GGDSSQLLSAL
Sbjct: 61  KPLCNGALSTFACSTSPFIGRVGSRIRLGNFSLLSFGLNSNAAAAAEGAGGDSSQLLSAL 120

Query: 121 LPFVVAATAIAALARPSTFTWVPKDLYAPALGGIMLSIGIKLSIDDFALAIKRPLPLSVG 180
           LPFVVAAT I+AL+ PSTF WVPKDLYAPALGGIMLSIGIKLSI+DFALA+KRP+PLSVG
Sbjct: 121 LPFVVAATGISALSWPSTFNWVPKDLYAPALGGIMLSIGIKLSINDFALAMKRPVPLSVG 180

Query: 181 FITQYGLKPALGILIARACGISPMFYAGFLLTACVGGAQLSSYASFLSKGDVALSILLTS 240
           F+ QY LKPALG+LIARA GISPMFYAGFLLTACVGGAQLSSYASFLSKGD+ALSILLTS
Sbjct: 181 FVAQYVLKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSILLTS 240

Query: 241 FTTISSVLLTPLLTGVLIGSVVPVDAVAMSKSILQVVLLPVTLGLVLNSYAKPIVAIIQP 300
           FTTISSVLLTPLLTG+LIGSVVPVDAVAMSKSILQVVLLPV LGLVLNSYAKPIVAI+QP
Sbjct: 241 FTTISSVLLTPLLTGILIGSVVPVDAVAMSKSILQVVLLPVALGLVLNSYAKPIVAILQP 300

Query: 301 VMPFVAMICTSLCIGSPLAINRSQILSIDGVRLVFPVLAFHTLAFTFGYWASKIPIIRQE 360
           VMPFVAM+CTSLCIGSPLAIN+SQILSIDG RLV PVL FHT+AFT GYWASKIPI+RQE
Sbjct: 301 VMPFVAMVCTSLCIGSPLAINKSQILSIDGARLVLPVLVFHTMAFTLGYWASKIPIVRQE 360

Query: 361 EEASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIR 420
           EEASRTISLCTGMQSSTLAGLLATQFLGST AVPPACSVV MAIMGLCLASFWGSGSKIR
Sbjct: 361 EEASRTISLCTGMQSSTLAGLLATQFLGSTHAVPPACSVVVMAIMGLCLASFWGSGSKIR 420

Query: 421 DLPSLLIQKTN 429
           DLPSLL+QKT+
Sbjct: 421 DLPSLLVQKTS 428

BLAST of MC09g0827 vs. ExPASy TrEMBL
Match: A0A6J1DPN9 (probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111023064 PE=3 SV=1)

HSP 1 Score: 801 bits (2068), Expect = 9.40e-292
Identity = 429/429 (100.00%), Postives = 429/429 (100.00%), Query Frame = 0

Query: 1   MFAIPLCSSPVPLLPQPPSPINHAKSKFHSSHCSYVSVSSISSSQSRLWSSSHCSTSKNC 60
           MFAIPLCSSPVPLLPQPPSPINHAKSKFHSSHCSYVSVSSISSSQSRLWSSSHCSTSKNC
Sbjct: 1   MFAIPLCSSPVPLLPQPPSPINHAKSKFHSSHCSYVSVSSISSSQSRLWSSSHCSTSKNC 60

Query: 61  RTASNGALFSFACSTSPFVGRVGSRIRQGNFSLLSFGLNSHAAADGGGGDSSQLLSALLP 120
           RTASNGALFSFACSTSPFVGRVGSRIRQGNFSLLSFGLNSHAAADGGGGDSSQLLSALLP
Sbjct: 61  RTASNGALFSFACSTSPFVGRVGSRIRQGNFSLLSFGLNSHAAADGGGGDSSQLLSALLP 120

Query: 121 FVVAATAIAALARPSTFTWVPKDLYAPALGGIMLSIGIKLSIDDFALAIKRPLPLSVGFI 180
           FVVAATAIAALARPSTFTWVPKDLYAPALGGIMLSIGIKLSIDDFALAIKRPLPLSVGFI
Sbjct: 121 FVVAATAIAALARPSTFTWVPKDLYAPALGGIMLSIGIKLSIDDFALAIKRPLPLSVGFI 180

Query: 181 TQYGLKPALGILIARACGISPMFYAGFLLTACVGGAQLSSYASFLSKGDVALSILLTSFT 240
           TQYGLKPALGILIARACGISPMFYAGFLLTACVGGAQLSSYASFLSKGDVALSILLTSFT
Sbjct: 181 TQYGLKPALGILIARACGISPMFYAGFLLTACVGGAQLSSYASFLSKGDVALSILLTSFT 240

Query: 241 TISSVLLTPLLTGVLIGSVVPVDAVAMSKSILQVVLLPVTLGLVLNSYAKPIVAIIQPVM 300
           TISSVLLTPLLTGVLIGSVVPVDAVAMSKSILQVVLLPVTLGLVLNSYAKPIVAIIQPVM
Sbjct: 241 TISSVLLTPLLTGVLIGSVVPVDAVAMSKSILQVVLLPVTLGLVLNSYAKPIVAIIQPVM 300

Query: 301 PFVAMICTSLCIGSPLAINRSQILSIDGVRLVFPVLAFHTLAFTFGYWASKIPIIRQEEE 360
           PFVAMICTSLCIGSPLAINRSQILSIDGVRLVFPVLAFHTLAFTFGYWASKIPIIRQEEE
Sbjct: 301 PFVAMICTSLCIGSPLAINRSQILSIDGVRLVFPVLAFHTLAFTFGYWASKIPIIRQEEE 360

Query: 361 ASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDL 420
           ASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDL
Sbjct: 361 ASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDL 420

Query: 421 PSLLIQKTN 429
           PSLLIQKTN
Sbjct: 421 PSLLIQKTN 429

BLAST of MC09g0827 vs. ExPASy TrEMBL
Match: A0A6J1JWY1 (probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111488209 PE=3 SV=1)

HSP 1 Score: 657 bits (1695), Expect = 6.29e-235
Identity = 363/429 (84.62%), Postives = 387/429 (90.21%), Query Frame = 0

Query: 1   MFAIPLCSSPVPLLPQPPSPINHAKSKFHSSHCSYVSVSSISSSQSRLWSSSHCSTSKNC 60
           M AI LCSSPVP   Q P      K K +SS C  +S S    + +RLWSS +CSTSKN 
Sbjct: 1   MSAIALCSSPVPFPSQLPIKPRR-KPKLYSSDCLCLSFSC---NLTRLWSS-YCSTSKNW 60

Query: 61  RTASNGALFSFACSTSPFVGRVGSRIRQGNFSLLSFGLNSHAAADGGGGDSSQLLSALLP 120
            T  NGA+ +FACSTSPFVGR+GSRIRQGNFSLLSFGLNS+A A+   GDSSQLLSALLP
Sbjct: 61  STVRNGAVSTFACSTSPFVGRIGSRIRQGNFSLLSFGLNSNAEAEATDGDSSQLLSALLP 120

Query: 121 FVVAATAIAALARPSTFTWVPKDLYAPALGGIMLSIGIKLSIDDFALAIKRPLPLSVGFI 180
           FVVAATAIAAL RPSTFTWV KD YAPALGGIMLSIGIKLSI+DFALAIKRPLPLSVGFI
Sbjct: 121 FVVAATAIAALTRPSTFTWVSKDFYAPALGGIMLSIGIKLSINDFALAIKRPLPLSVGFI 180

Query: 181 TQYGLKPALGILIARACGISPMFYAGFLLTACVGGAQLSSYASFLSKGDVALSILLTSFT 240
            QY LKPALG+LIARA GISPMFYAGFLLTACVGGAQLSSYASFLSKGD+ALS+LLTSFT
Sbjct: 181 AQYVLKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSVLLTSFT 240

Query: 241 TISSVLLTPLLTGVLIGSVVPVDAVAMSKSILQVVLLPVTLGLVLNSYAKPIVAIIQPVM 300
           TISSVLLTPLLTG+LIGSVVPVDAVAMSKSILQVVL+P++LGLVLNSYAKPIVA++QPVM
Sbjct: 241 TISSVLLTPLLTGILIGSVVPVDAVAMSKSILQVVLVPISLGLVLNSYAKPIVALLQPVM 300

Query: 301 PFVAMICTSLCIGSPLAINRSQILSIDGVRLVFPVLAFHTLAFTFGYWASKIPIIRQEEE 360
           PFVAMICTSLCIGSPLAINRSQILSIDG RLVFPVL FHT+AFT GYWASKIPI+RQEEE
Sbjct: 301 PFVAMICTSLCIGSPLAINRSQILSIDGARLVFPVLVFHTMAFTLGYWASKIPIVRQEEE 360

Query: 361 ASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDL 420
           ASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDL
Sbjct: 361 ASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDL 420

Query: 421 PSLLIQKTN 429
           PSLL+QKT+
Sbjct: 421 PSLLMQKTS 424

BLAST of MC09g0827 vs. ExPASy TrEMBL
Match: A0A5D3D222 (Putative sodium/metabolite cotransporter BASS3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold306G002340 PE=3 SV=1)

HSP 1 Score: 654 bits (1688), Expect = 8.17e-234
Identity = 361/432 (83.56%), Postives = 386/432 (89.35%), Query Frame = 0

Query: 1   MFAIPLCSSPVPLLPQPPSPINHA-KSKFHSSHCSYVSVSSISSSQSRLWSSSHCSTSKN 60
           M  IPLCSSPVP L Q  SPI  + K KF SS C  +S+S    + +RLW SSHCSTSKN
Sbjct: 1   MSPIPLCSSPVPFLSQ--SPIKPSRKPKFFSSDCHCLSISC---TFTRLWPSSHCSTSKN 60

Query: 61  CRTASNGALFSFACSTSPFVGRVGSRIRQGNFSLLSFGLNSHAAA--DGGGGDSSQLLSA 120
            +   NGAL +FACSTSPF+GRVGSRIR GNFSLLSFGLNS+AAA  +G GGDSSQ  S 
Sbjct: 61  LKPVCNGALSTFACSTSPFIGRVGSRIRLGNFSLLSFGLNSNAAAAEEGAGGDSSQFFSG 120

Query: 121 LLPFVVAATAIAALARPSTFTWVPKDLYAPALGGIMLSIGIKLSIDDFALAIKRPLPLSV 180
           LLPFVVAAT I+AL  PSTFTWVPKDLYAPALGGIMLSIGIKLSI+DFALA+KRP+PLS+
Sbjct: 121 LLPFVVAATGISALTCPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAMKRPVPLSI 180

Query: 181 GFITQYGLKPALGILIARACGISPMFYAGFLLTACVGGAQLSSYASFLSKGDVALSILLT 240
           GF+ QY LKPALG+LIARA GISP+FYAGFLLTACVGGAQLSSYASFLSKGD+ALSILLT
Sbjct: 181 GFVAQYVLKPALGVLIARAFGISPLFYAGFLLTACVGGAQLSSYASFLSKGDIALSILLT 240

Query: 241 SFTTISSVLLTPLLTGVLIGSVVPVDAVAMSKSILQVVLLPVTLGLVLNSYAKPIVAIIQ 300
           SFTTISSVLLTPLLTG+LIGSVVPVDA AMSKSILQVVLLPV LGLVLNSYAKPIVAIIQ
Sbjct: 241 SFTTISSVLLTPLLTGILIGSVVPVDAAAMSKSILQVVLLPVALGLVLNSYAKPIVAIIQ 300

Query: 301 PVMPFVAMICTSLCIGSPLAINRSQILSIDGVRLVFPVLAFHTLAFTFGYWASKIPIIRQ 360
           PVMPFVA+ICTSLCIGSPLAINRSQILSI G +LV PVLAFH +AFT GYWASKIPIIRQ
Sbjct: 301 PVMPFVAIICTSLCIGSPLAINRSQILSIHGAQLVLPVLAFHAMAFTLGYWASKIPIIRQ 360

Query: 361 EEEASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKI 420
           EEEASRTISLCTGMQSSTLAGLLATQFLGST AVPPACSVVAMAIMGLCLASFWGSGSKI
Sbjct: 361 EEEASRTISLCTGMQSSTLAGLLATQFLGSTHAVPPACSVVAMAIMGLCLASFWGSGSKI 420

Query: 421 RDLPSLLIQKTN 429
           RDLPSLL+QKT+
Sbjct: 421 RDLPSLLVQKTS 427

BLAST of MC09g0827 vs. ExPASy TrEMBL
Match: A0A1S3BGX9 (probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103489720 PE=3 SV=1)

HSP 1 Score: 654 bits (1687), Expect = 1.16e-233
Identity = 361/432 (83.56%), Postives = 386/432 (89.35%), Query Frame = 0

Query: 1   MFAIPLCSSPVPLLPQPPSPINHA-KSKFHSSHCSYVSVSSISSSQSRLWSSSHCSTSKN 60
           M AIPLCSSPVP L Q  SPI  + K KF SS C  +S+S    + +RLW  SHCSTSKN
Sbjct: 1   MSAIPLCSSPVPFLSQ--SPIKPSRKPKFFSSDCHCLSISC---TFTRLWPFSHCSTSKN 60

Query: 61  CRTASNGALFSFACSTSPFVGRVGSRIRQGNFSLLSFGLNSHAAA--DGGGGDSSQLLSA 120
            +   NGAL +FACSTSPF+GRVGSRIR GNFSLLSFGLNS+AAA  +G GGDSSQ  S 
Sbjct: 61  LKPVCNGALSTFACSTSPFIGRVGSRIRLGNFSLLSFGLNSNAAAAEEGAGGDSSQFFSG 120

Query: 121 LLPFVVAATAIAALARPSTFTWVPKDLYAPALGGIMLSIGIKLSIDDFALAIKRPLPLSV 180
           LLPFVVAAT I+AL  PSTFTWVPKDLYAPALGGIMLSIGIKLSI+DFALA+KRP+PLS+
Sbjct: 121 LLPFVVAATGISALTCPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAMKRPVPLSI 180

Query: 181 GFITQYGLKPALGILIARACGISPMFYAGFLLTACVGGAQLSSYASFLSKGDVALSILLT 240
           GF+ QY LKPALG+LIARA GISP+FYAGFLLTACVGGAQLSSYASFLSKGD+ALSILLT
Sbjct: 181 GFVAQYVLKPALGVLIARAFGISPLFYAGFLLTACVGGAQLSSYASFLSKGDIALSILLT 240

Query: 241 SFTTISSVLLTPLLTGVLIGSVVPVDAVAMSKSILQVVLLPVTLGLVLNSYAKPIVAIIQ 300
           SFTTISSVLLTPLLTG+LIGSVVPVDA AMSKSILQVVLLPV LGLVLNSYAKPIVAIIQ
Sbjct: 241 SFTTISSVLLTPLLTGILIGSVVPVDAAAMSKSILQVVLLPVALGLVLNSYAKPIVAIIQ 300

Query: 301 PVMPFVAMICTSLCIGSPLAINRSQILSIDGVRLVFPVLAFHTLAFTFGYWASKIPIIRQ 360
           PVMPFVA+ICTSLCIGSPLAINRSQILSI G +LV PVLAFH +AFT GYWASKIPIIRQ
Sbjct: 301 PVMPFVAIICTSLCIGSPLAINRSQILSIHGAQLVLPVLAFHAMAFTLGYWASKIPIIRQ 360

Query: 361 EEEASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKI 420
           EEEASRTISLCTGMQSSTLAGLLATQFLGST AVPPACSVVAMAIMGLCLASFWGSGSKI
Sbjct: 361 EEEASRTISLCTGMQSSTLAGLLATQFLGSTHAVPPACSVVAMAIMGLCLASFWGSGSKI 420

Query: 421 RDLPSLLIQKTN 429
           RDLPSLL+QKT+
Sbjct: 421 RDLPSLLVQKTS 427

BLAST of MC09g0827 vs. ExPASy TrEMBL
Match: A0A6J1GQ81 (probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111456499 PE=3 SV=1)

HSP 1 Score: 652 bits (1681), Expect = 8.50e-233
Identity = 361/429 (84.15%), Postives = 385/429 (89.74%), Query Frame = 0

Query: 1   MFAIPLCSSPVPLLPQPPSPINHAKSKFHSSHCSYVSVSSISSSQSRLWSSSHCSTSKNC 60
           M AI LCSSPVP   Q P      K K +SS C  +S S    + +RLWSS HCSTSKN 
Sbjct: 1   MSAIALCSSPVPFPSQLPIKPRR-KPKLYSSDCLCLSFSC---NLTRLWSS-HCSTSKNW 60

Query: 61  RTASNGALFSFACSTSPFVGRVGSRIRQGNFSLLSFGLNSHAAADGGGGDSSQLLSALLP 120
            T  NGA+ +FACSTS F GR+GSRIRQGN SLLSFGLNS+A A+   GDSSQLLSALLP
Sbjct: 61  STVRNGAVSTFACSTSSFAGRIGSRIRQGNSSLLSFGLNSNAEAEATDGDSSQLLSALLP 120

Query: 121 FVVAATAIAALARPSTFTWVPKDLYAPALGGIMLSIGIKLSIDDFALAIKRPLPLSVGFI 180
           FVVAATAIAAL RPSTFTWV KD YAPALGGIMLSIGIKLSI+DFALAIKRPLPLSVGFI
Sbjct: 121 FVVAATAIAALTRPSTFTWVSKDFYAPALGGIMLSIGIKLSINDFALAIKRPLPLSVGFI 180

Query: 181 TQYGLKPALGILIARACGISPMFYAGFLLTACVGGAQLSSYASFLSKGDVALSILLTSFT 240
            QY LKPALG+LIARA GISPMFYAGFLLTACVGGAQLSSYASFLSKGD+ALS+LLTSFT
Sbjct: 181 AQYVLKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSVLLTSFT 240

Query: 241 TISSVLLTPLLTGVLIGSVVPVDAVAMSKSILQVVLLPVTLGLVLNSYAKPIVAIIQPVM 300
           TISSVLLTPLLTG+LIGSVVPVDAVAMSKSILQVVL+P++LGLVLNSYAKPIVA++QPVM
Sbjct: 241 TISSVLLTPLLTGILIGSVVPVDAVAMSKSILQVVLVPISLGLVLNSYAKPIVALLQPVM 300

Query: 301 PFVAMICTSLCIGSPLAINRSQILSIDGVRLVFPVLAFHTLAFTFGYWASKIPIIRQEEE 360
           PFVAMICTSLCIGSPLAINRSQILSIDG RLVFPVLAFHT+AFT GYWASKIPI+RQEEE
Sbjct: 301 PFVAMICTSLCIGSPLAINRSQILSIDGARLVFPVLAFHTMAFTLGYWASKIPIVRQEEE 360

Query: 361 ASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDL 420
           ASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDL
Sbjct: 361 ASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDL 420

Query: 421 PSLLIQKTN 429
           PSLL++KT+
Sbjct: 421 PSLLMKKTS 424

BLAST of MC09g0827 vs. TAIR 10
Match: AT3G25410.1 (Sodium Bile acid symporter family )

HSP 1 Score: 487.3 bits (1253), Expect = 1.3e-137
Identity = 275/412 (66.75%), Postives = 323/412 (78.40%), Query Frame = 0

Query: 17  PPSPINHAKSKFHSSHCSYVSVSSISSSQSRLWSSSHCSTSKN-----CRTASNGALFSF 76
           PP+    + S+FH+      S +    S S   SS H S+  N      R  ++G +   
Sbjct: 11  PPAIQRQSLSEFHNLPAKSQSFNCQFRSSSSPLSSLHSSSLSNFLDFRLRRRNSGLVPVV 70

Query: 77  ACSTSPFVGRVGSRIRQGNFSLLSFGLNSHAAADGGGGDSSQLLSALLPFVVAATAIAAL 136
           ACST+PF+GRVG   R GN SLLSF   +         DSSQ  SALLPFVVA TA+AAL
Sbjct: 71  ACSTTPFMGRVGLHWRDGNMSLLSFCGGTDVTEK---ADSSQFWSALLPFVVALTAVAAL 130

Query: 137 ARPSTFTWVPKDLYAPALGGIMLSIGIKLSIDDFALAIKRPLPLSVGFITQYGLKPALGI 196
           + P +FTWV KDLYAPALGGIMLSIGI+LS+DDFALA KRP+PLSVGF+ QY LKP LG+
Sbjct: 131 SYPPSFTWVSKDLYAPALGGIMLSIGIQLSVDDFALAFKRPVPLSVGFVAQYVLKPLLGV 190

Query: 197 LIARACGISPMFYAGFLLTACVGGAQLSSYASFLSKGDVALSILLTSFTTISSVLLTPLL 256
           L+A A G+   FYAGF+LT CV GAQLSSYAS LSK DVA+SILLTS TTI+SV+ TPLL
Sbjct: 191 LVANAFGMPRTFYAGFILTCCVAGAQLSSYASSLSKADVAMSILLTSSTTIASVIFTPLL 250

Query: 257 TGVLIGSVVPVDAVAMSKSILQVVLLPVTLGLVLNSYAKPIVAIIQPVMPFVAMICTSLC 316
           +G+LIGSVVPVDAVAMSKSILQVVL+P+TLGLVLN+YAKP+V ++QPVMPFVAM+CTSLC
Sbjct: 251 SGLLIGSVVPVDAVAMSKSILQVVLVPITLGLVLNTYAKPVVTLLQPVMPFVAMVCTSLC 310

Query: 317 IGSPLAINRSQILSIDGVRLVFPVLAFHTLAFTFGYWASKIPIIRQEEEASRTISLCTGM 376
           IGSPL+INRSQILS +G+ L+ P++ FH +AF  GYW SKIP +RQEEE SRTISLCTGM
Sbjct: 311 IGSPLSINRSQILSAEGLGLIVPIVTFHAVAFALGYWFSKIPGLRQEEEVSRTISLCTGM 370

Query: 377 QSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDLPSL 424
           QSSTLAGLLA+QFLGS+QAVP ACSVV MAIMGLCLASFWG+G +IRD+ SL
Sbjct: 371 QSSTLAGLLASQFLGSSQAVPAACSVVVMAIMGLCLASFWGNGFRIRDVLSL 419

BLAST of MC09g0827 vs. TAIR 10
Match: AT2G26900.1 (Sodium Bile acid symporter family )

HSP 1 Score: 203.0 bits (515), Expect = 4.9e-52
Identity = 123/300 (41.00%), Postives = 177/300 (59.00%), Query Frame = 0

Query: 113 QLLSALLPFVVAATAIAALARPSTFTWVPKDLYAPALGGIMLSIGIKLSIDDFALAIKRP 172
           +LL+ L P  V    +  + +PS  TW+  DL+   LG +MLS+G+ L+ +DF   ++ P
Sbjct: 99  ELLTTLFPLWVILGTLVGIFKPSLVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNP 158

Query: 173 LPLSVGFITQYGLKPALGILIARACGISPMFYAGFLLTACVGGAQLSSYASFLSKGDVAL 232
             + VGF+ QY +KP LG LIA    +S     G +L +C  G Q S+ A+++SKG+VAL
Sbjct: 159 WTVGVGFLAQYMIKPILGFLIAMTLKLSAPLATGLILVSCCPGGQASNVATYISKGNVAL 218

Query: 233 SILLTSFTTISSVLLTPLLTGVLIGSVVPVDAVAMSKSILQVVLLPVTLGLVLNSYAKPI 292
           S+L+T+ +TI ++++TPLLT +L G +VPVDA  ++ S  QVVL+P  +G++ N +    
Sbjct: 219 SVLMTTCSTIGAIIMTPLLTKLLAGQLVPVDAAGLALSTFQVVLVPTIIGVLANEFFPKF 278

Query: 293 VAIIQPVMPFVAMICTSLCIGSPLAINRSQILSIDGVRLVFPVLAFHTLAFTFGYWASKI 352
            + I  V P + +I T+L   SP+    + +L   G +L+ PV   H  AF  GYW SK 
Sbjct: 279 TSKIITVTPLIGVILTTLLCASPIG-QVADVLKTQGAQLILPVALLHAAAFAIGYWISKF 338

Query: 353 PIIRQEEEASRTISLCTGMQSSTLAGLLATQ-FLGSTQAVPPACSVVAMAIMGLCLASFW 412
                 E  SRTIS+  GMQSS L  LLA + F     AVP A SVV MA+ G  LA FW
Sbjct: 339 SF---GESTSRTISIECGMQSSALGFLLAQKHFTNPLVAVPSAVSVVCMALGGSGLAVFW 394

BLAST of MC09g0827 vs. TAIR 10
Match: AT4G22840.1 (Sodium Bile acid symporter family )

HSP 1 Score: 192.2 bits (487), Expect = 8.6e-49
Identity = 124/311 (39.87%), Postives = 186/311 (59.81%), Query Frame = 0

Query: 110 DSSQLLSALLPFVVAATAIAALARPSTFTWVPKDLYAPALGGIMLSIGIKLSIDDFALAI 169
           D  +  +++LP VV A+ I AL  P +FTW     + PALG +M ++GI  +  DF  A 
Sbjct: 92  DRMKKANSILPHVVLASTILALIYPPSFTWFTSRYFVPALGFLMFAVGINSNEKDFLEAF 151

Query: 170 KRPLPLSVGFITQYGLKPALGILIARAC----GISPMFYAGFLLTACVGGAQLSSYASFL 229
           KRP  + +G++ QY +KP LG +   A      +     AG +L +CV GAQLS+YA+FL
Sbjct: 152 KRPKAILLGYVGQYLVKPVLGFIFGLAAVSLFQLPTPIGAGIMLVSCVSGAQLSNYATFL 211

Query: 230 SKGDVA-LSILLTSFTTISSVLLTPLLTGVLIGSVVPVDAVAMSKSILQVVLLPVTLGLV 289
           +   +A LSI++TS +T ++VL+TP+L+ +LIG  +PVD   M  SILQVV+ P+  GL+
Sbjct: 212 TDPALAPLSIVMTSLSTATAVLVTPMLSLLLIGKKLPVDVKGMISSILQVVIAPIAAGLL 271

Query: 290 LNSYAKPIVAIIQPVMPFVAMICTSLCIGSPLAINRSQILSIDGVRLVFPVLAFHTLAFT 349
           LN     +   I+P +P ++++ T+ C+G+PLA+N + ++S  G  ++  V  FH  AF 
Sbjct: 272 LNKLFPKVSNAIRPFLPILSVLDTACCVGAPLALNINSVMSPFGATILLLVTMFHLSAFL 331

Query: 350 FGYWASKIPIIRQEEEA---SRTISLCTGMQSSTLAGLLATQFL-GSTQAVPPACSVVAM 409
            GY+ +   + R   +A    RT+S  TGMQSS LA  LAT+F       +PPA S V M
Sbjct: 332 AGYFLTG-SVFRNAPDAKAMQRTLSYETGMQSSLLALALATKFFQDPLVGIPPAISTVVM 391

Query: 410 AIMGLCLASFW 412
           ++MG  L   W
Sbjct: 392 SLMGFTLVLIW 401

BLAST of MC09g0827 vs. TAIR 10
Match: AT4G12030.2 (bile acid transporter 5 )

HSP 1 Score: 188.7 bits (478), Expect = 9.5e-48
Identity = 126/306 (41.18%), Postives = 180/306 (58.82%), Query Frame = 0

Query: 117 ALLPFVVAATAIAALARPSTFTWVPKDLYAPALGGIMLSIGIKLSIDDFALAIKRPLPLS 176
           + +P  +  + I AL  P +FTW     + P LG +M ++GI  +  DF  A+KRP  + 
Sbjct: 100 SFIPHGILLSTILALVYPPSFTWFKPRYFVPGLGFMMFAVGINSNERDFLEALKRPDAIF 159

Query: 177 VGFITQYGLKPAL----GILIARACGISPMFYAGFLLTACVGGAQLSSYASFLSKGDV-A 236
            G+I QY +KP L    G++      +     AG +L +CV GAQLS+Y +FL+   + A
Sbjct: 160 AGYIGQYLIKPLLGYIFGVIAVSLFNLPTSIGAGIMLVSCVSGAQLSNYTTFLTDPSLAA 219

Query: 237 LSILLTSFTTISSVLLTPLLTGVLIGSVVPVDAVAMSKSILQVVLLPVTLGLVLNSYAKP 296
           LSI++TS +T ++VL+TP+L+ +LIG  +PVD   M  SILQVV+ P+  GL+LN     
Sbjct: 220 LSIVMTSISTATAVLVTPMLSLLLIGKKLPVDVFGMISSILQVVITPIAAGLLLNRLFPR 279

Query: 297 IVAIIQPVMPFVAMICTSLCIGSPLAINRSQILSIDGVRLVFPVLAFHTLAFTFGY---- 356
           +   I+P +P + +I  S CIG+PLA+N   ILS  G  ++F V+ FH LAF  GY    
Sbjct: 280 LSNAIKPFLPALTVIDMSCCIGAPLALNIDSILSPFGATILFLVITFHLLAFVAGYFFTG 339

Query: 357 -WASKIPIIRQEEEASRTISLCTGMQSSTLAGLLATQFL-GSTQAVPPACSVVAMAIMGL 412
            + SK P ++      RTIS  TGMQSS LA  LAT+F       VPPA S V M++MG+
Sbjct: 340 FFFSKAPDVK---ALQRTISYETGMQSSLLALALATKFFQDPLVGVPPAISTVVMSLMGV 399

BLAST of MC09g0827 vs. TAIR 10
Match: AT1G78560.1 (Sodium Bile acid symporter family )

HSP 1 Score: 169.5 bits (428), Expect = 6.0e-42
Identity = 112/300 (37.33%), Postives = 168/300 (56.00%), Query Frame = 0

Query: 113 QLLSALLPFVVAATAIAALARPSTFTWVPKDLYAPALGGIMLSIGIKLSIDDFALAIKRP 172
           + +S   P  V+   +  L RPSTF WV  +     L   ML +G+ L++DD   A+  P
Sbjct: 95  EAVSTAFPIWVSLGCLLGLMRPSTFNWVTPNWTIVGLTITMLGMGMTLTLDDLRGALSMP 154

Query: 173 LPLSVGFITQYGLKPALGILIARACGISPMFYAGFLLTACVGGAQLSSYASFLSKGDVAL 232
             L  GF+ QY + P     +++   + P + AG +L  C  G   S+  +++++G+VAL
Sbjct: 155 KELFAGFLLQYSVMPLSAFFVSKLLNLPPHYAAGLILVGCCPGGTASNIVTYIARGNVAL 214

Query: 233 SILLTSFTTISSVLLTPLLTGVLIGSVVPVDAVAMSKSILQVVLLPVTLGLVLNSYAKPI 292
           S+L+T+ +T+S+V++TPLLT  L    + VDA+ +  S LQVVLLPV  G  LN Y K +
Sbjct: 215 SVLMTAASTVSAVIMTPLLTAKLAKQYITVDALGLLMSTLQVVLLPVLAGAFLNQYFKKL 274

Query: 293 VAIIQPVMPFVAMICTSLCIGSPLAINRSQILSIDGVRLVFPVLAFHTLAFTFGYWASKI 352
           V  + PVMP +A+   ++  G  +  N S IL + G ++V      H   F FGY  S+ 
Sbjct: 275 VKFVSPVMPPIAVGTVAILCGYAIGQNASAIL-MSGKQVVLASCLLHISGFLFGYLFSR- 334

Query: 353 PIIRQEEEASRTISLCTGMQSSTLAGLLATQFLGS-TQAVPPACSVVAMAIMGLCLASFW 412
            I+  +  +SRTIS+  GMQ+S L  +LATQ  G+   AVP A S V  +I+G  LA  W
Sbjct: 335 -ILGIDVASSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSILGSVLAGIW 391

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8RXE81.8e-13666.75Probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Arabidopsis tha... [more]
Q6K7391.0e-12362.80Probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Oryza sativa su... [more]
Q1EBV76.9e-5141.00Sodium/pyruvate cotransporter BASS2, chloroplastic OS=Arabidopsis thaliana OX=37... [more]
Q5VRB22.6e-5041.00Probable sodium/metabolite cotransporter BASS2, chloroplastic OS=Oryza sativa su... [more]
B8BDK41.7e-4940.71Probable sodium/metabolite cotransporter BASS5, chloroplastic OS=Oryza sativa su... [more]
Match NameE-valueIdentityDescription
XP_022156093.11.94e-291100.00probable sodium/metabolite cotransporter BASS3, chloroplastic [Momordica charant... [more]
XP_038899090.11.08e-23886.01probable sodium/metabolite cotransporter BASS3, chloroplastic [Benincasa hispida... [more]
XP_022991663.11.30e-23484.62probable sodium/metabolite cotransporter BASS3, chloroplastic [Cucurbita maxima][more]
XP_023549213.12.62e-23484.62probable sodium/metabolite cotransporter BASS3, chloroplastic [Cucurbita pepo su... [more]
XP_004150363.14.15e-23483.06probable sodium/metabolite cotransporter BASS3, chloroplastic [Cucumis sativus] ... [more]
Match NameE-valueIdentityDescription
A0A6J1DPN99.40e-292100.00probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Momordica chara... [more]
A0A6J1JWY16.29e-23584.62probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Cucurbita maxim... [more]
A0A5D3D2228.17e-23483.56Putative sodium/metabolite cotransporter BASS3 OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3BGX91.16e-23383.56probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Cucumis melo OX... [more]
A0A6J1GQ818.50e-23384.15probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Cucurbita mosch... [more]
Match NameE-valueIdentityDescription
AT3G25410.11.3e-13766.75Sodium Bile acid symporter family [more]
AT2G26900.14.9e-5241.00Sodium Bile acid symporter family [more]
AT4G22840.18.6e-4939.87Sodium Bile acid symporter family [more]
AT4G12030.29.5e-4841.18bile acid transporter 5 [more]
AT1G78560.16.0e-4237.33Sodium Bile acid symporter family [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002657Bile acid:sodium symporter/arsenical resistance protein Acr3PFAMPF01758SBFcoord: 147..325
e-value: 1.1E-29
score: 103.5
IPR038770Sodium/solute symporter superfamilyGENE3D1.20.1530.20coord: 110..413
e-value: 1.1E-67
score: 230.4
IPR004710Bile acid:sodium symporterPANTHERPTHR10361SODIUM-BILE ACID COTRANSPORTERcoord: 25..428
NoneNo IPR availablePANTHERPTHR10361:SF33SODIUM/METABOLITE COTRANSPORTER BASS3, CHLOROPLASTIC-RELATEDcoord: 25..428

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g0827.1MC09g0827.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0009941 chloroplast envelope
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane