MC09g0819 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g0819
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionProtease inhibitor 2
LocationMC09: 8473945 .. 8474145 (-)
RNA-Seq ExpressionMC09g0819
SyntenyMC09g0819
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTGTGCAGGGAAGATGGCGTGGCCGGAGCTGTTGGGAGTGAGACGCGGGGAGGCGAAGAATAGGATAGAGAGGGAGAATCCGTACGTGGACGTGGCCGTGATCCCAGAAGGGAGGATCGTGACCTTCGATCTGAGGTGCGATAGGGTTCGGATTTGGGTTAGCCCAAACGGAATCGTGACTCGCATTCCGGTGGTTGGG

mRNA sequence

GTTTGTGCAGGGAAGATGGCGTGGCCGGAGCTGTTGGGAGTGAGACGCGGGGAGGCGAAGAATAGGATAGAGAGGGAGAATCCGTACGTGGACGTGGCCGTGATCCCAGAAGGGAGGATCGTGACCTTCGATCTGAGGTGCGATAGGGTTCGGATTTGGGTTAGCCCAAACGGAATCGTGACTCGCATTCCGGTGGTTGGG

Coding sequence (CDS)

GTTTGTGCAGGGAAGATGGCGTGGCCGGAGCTGTTGGGAGTGAGACGCGGGGAGGCGAAGAATAGGATAGAGAGGGAGAATCCGTACGTGGACGTGGCCGTGATCCCAGAAGGGAGGATCGTGACCTTCGATCTGAGGTGCGATAGGGTTCGGATTTGGGTTAGCCCAAACGGAATCGTGACTCGCATTCCGGTGGTTGGG

Protein sequence

VCAGKMAWPELLGVRRGEAKNRIERENPYVDVAVIPEGRIVTFDLRCDRVRIWVSPNGIVTRIPVVG
Homology
BLAST of MC09g0819 vs. ExPASy Swiss-Prot
Match: P24076 (Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 1.5e-13
Identity = 34/66 (51.52%), Postives = 42/66 (63.64%), Query Frame = 0

Query: 2  CAGKMAWPELLGVRRGEAKNRIERENPYVDVAVIPEGRIVTFDLRCDRVRIWVSPNGIVT 61
          C GK +WP+L+G     AK  IERENP V   ++  G  VT D RCDRVR+WV+  GIV 
Sbjct: 3  CQGKRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVGSPVTADFRCDRVRVWVTERGIVA 62

Query: 62 RIPVVG 68
          R P +G
Sbjct: 63 RPPAIG 68

BLAST of MC09g0819 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 1.7e-12
Identity = 33/66 (50.00%), Postives = 42/66 (63.64%), Query Frame = 0

Query: 2  CAGKMAWPELLGVRRGEAKNRIERENPYVDVAVIPEGRIVTFDLRCDRVRIWVSPNGIVT 61
          C GK +WP L+GV    AK  IER+NP V   ++ EG  VT D RC+RVRIWV+  G+V 
Sbjct: 3  CPGKSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVV 62

Query: 62 RIPVVG 68
            P +G
Sbjct: 63 SPPRIG 68

BLAST of MC09g0819 vs. ExPASy Swiss-Prot
Match: Q00783 (Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 2.7e-10
Identity = 33/67 (49.25%), Postives = 44/67 (65.67%), Query Frame = 0

Query: 2   CAGKMAWPELLGVRRGEAKNRIERENPYV-DVAVIPEGRIVTFDLRCDRVRIWVSPNGIV 61
           C GK+ WPEL+GV    AK  IE+EN  + +V ++  G  VT D+RCDRVR++ +  G V
Sbjct: 41  CKGKLRWPELIGVPTKLAKGIIEKENSLISNVHILLNGSPVTLDIRCDRVRLFDNILGYV 100

Query: 62  TRIPVVG 68
             IPVVG
Sbjct: 101 VDIPVVG 107

BLAST of MC09g0819 vs. ExPASy Swiss-Prot
Match: P82381 (Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 3.5e-10
Identity = 30/63 (47.62%), Postives = 39/63 (61.90%), Query Frame = 0

Query: 2  CAGKMAWPELLGVRRGEAKNRIERENPYVDVAVIPEGRIVTFDLRCDRVRIWVSPNGIVT 61
          C GK AWPEL+G     A   +EREN  V   V+ EG  +T D RCDRV + V+ +G+VT
Sbjct: 4  CPGKNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKDFRCDRVWVIVNDHGVVT 63

Query: 62 RIP 65
           +P
Sbjct: 64 SVP 66

BLAST of MC09g0819 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 1.7e-09
Identity = 32/66 (48.48%), Postives = 43/66 (65.15%), Query Frame = 0

Query: 2   CAGKMAWPELLGVRRGEAKNRIERENPYV-DVAVIPEGRIVTFDLRCDRVRIWVSPNGIV 61
           C GK  WPEL+GV    AK  IE+ENP + ++ ++  G  VT D RCDRVR++V+  G V
Sbjct: 45  CKGKQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDV 104

Query: 62  TRIPVV 67
            +IP V
Sbjct: 105 VQIPRV 110

BLAST of MC09g0819 vs. NCBI nr
Match: KGN44697.1 (hypothetical protein Csa_016107 [Cucumis sativus])

HSP 1 Score: 112 bits (279), Expect = 1.31e-30
Identity = 50/66 (75.76%), Postives = 58/66 (87.88%), Query Frame = 0

Query: 2  CAGKMAWPELLGVRRGEAKNRIERENPYVDVAVIPEGRIVTFDLRCDRVRIWVSPNGIVT 61
          C GKMAWPELLG R  EAK+RI +ENPYVDVAVI EGR+VTFD+RCDRVR+WV+ +G+VT
Sbjct: 5  CRGKMAWPELLGTRSEEAKSRIVKENPYVDVAVIREGRVVTFDIRCDRVRVWVNRDGVVT 64

Query: 62 RIPVVG 67
          RIP VG
Sbjct: 65 RIPFVG 70

BLAST of MC09g0819 vs. NCBI nr
Match: XP_022953422.1 (glu S.griseus protease inhibitor-like isoform X2 [Cucurbita moschata] >XP_023547558.1 glu S.griseus protease inhibitor-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 108 bits (269), Expect = 4.39e-29
Identity = 47/66 (71.21%), Postives = 56/66 (84.85%), Query Frame = 0

Query: 2  CAGKMAWPELLGVRRGEAKNRIERENPYVDVAVIPEGRIVTFDLRCDRVRIWVSPNGIVT 61
          C GKM WPELLG R  EA++RIE+ENPYVD A++ EGR+VT DLRCDRVRIWV+ +GIVT
Sbjct: 5  CRGKMMWPELLGARGEEARSRIEKENPYVDAAIVREGRVVTLDLRCDRVRIWVNEDGIVT 64

Query: 62 RIPVVG 67
          R+P VG
Sbjct: 65 RVPFVG 70

BLAST of MC09g0819 vs. NCBI nr
Match: XP_022991208.1 (glu S.griseus protease inhibitor-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 107 bits (266), Expect = 1.26e-28
Identity = 45/66 (68.18%), Postives = 56/66 (84.85%), Query Frame = 0

Query: 2  CAGKMAWPELLGVRRGEAKNRIERENPYVDVAVIPEGRIVTFDLRCDRVRIWVSPNGIVT 61
          C GKM WPELLG R  EA++RIE+ENPYVD A++ EGR+VT D+RCDRVR+WV+ +GIVT
Sbjct: 5  CRGKMMWPELLGARGEEARSRIEKENPYVDAAIVREGRVVTLDIRCDRVRVWVNEDGIVT 64

Query: 62 RIPVVG 67
          R+P VG
Sbjct: 65 RVPFVG 70

BLAST of MC09g0819 vs. NCBI nr
Match: XP_022953421.1 (glu S.griseus protease inhibitor-like isoform X1 [Cucurbita moschata] >XP_023547557.1 glu S.griseus protease inhibitor-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 106 bits (265), Expect = 1.84e-28
Identity = 47/65 (72.31%), Postives = 56/65 (86.15%), Query Frame = 0

Query: 3  AGKMAWPELLGVRRGEAKNRIERENPYVDVAVIPEGRIVTFDLRCDRVRIWVSPNGIVTR 62
          AGKM WPELLG R  EA++RIE+ENPYVD A++ EGR+VT DLRCDRVRIWV+ +GIVTR
Sbjct: 7  AGKMMWPELLGARGEEARSRIEKENPYVDAAIVREGRVVTLDLRCDRVRIWVNEDGIVTR 66

Query: 63 IPVVG 67
          +P VG
Sbjct: 67 VPFVG 71

BLAST of MC09g0819 vs. NCBI nr
Match: XP_022991207.1 (glu S.griseus protease inhibitor-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 105 bits (262), Expect = 5.28e-28
Identity = 45/65 (69.23%), Postives = 56/65 (86.15%), Query Frame = 0

Query: 3  AGKMAWPELLGVRRGEAKNRIERENPYVDVAVIPEGRIVTFDLRCDRVRIWVSPNGIVTR 62
          AGKM WPELLG R  EA++RIE+ENPYVD A++ EGR+VT D+RCDRVR+WV+ +GIVTR
Sbjct: 7  AGKMMWPELLGARGEEARSRIEKENPYVDAAIVREGRVVTLDIRCDRVRVWVNEDGIVTR 66

Query: 63 IPVVG 67
          +P VG
Sbjct: 67 VPFVG 71

BLAST of MC09g0819 vs. ExPASy TrEMBL
Match: A0A0A0K8J9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G372910 PE=3 SV=1)

HSP 1 Score: 112 bits (279), Expect = 6.33e-31
Identity = 50/66 (75.76%), Postives = 58/66 (87.88%), Query Frame = 0

Query: 2  CAGKMAWPELLGVRRGEAKNRIERENPYVDVAVIPEGRIVTFDLRCDRVRIWVSPNGIVT 61
          C GKMAWPELLG R  EAK+RI +ENPYVDVAVI EGR+VTFD+RCDRVR+WV+ +G+VT
Sbjct: 5  CRGKMAWPELLGTRSEEAKSRIVKENPYVDVAVIREGRVVTFDIRCDRVRVWVNRDGVVT 64

Query: 62 RIPVVG 67
          RIP VG
Sbjct: 65 RIPFVG 70

BLAST of MC09g0819 vs. ExPASy TrEMBL
Match: A0A6J1GN66 (glu S.griseus protease inhibitor-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111455981 PE=3 SV=1)

HSP 1 Score: 108 bits (269), Expect = 2.13e-29
Identity = 47/66 (71.21%), Postives = 56/66 (84.85%), Query Frame = 0

Query: 2  CAGKMAWPELLGVRRGEAKNRIERENPYVDVAVIPEGRIVTFDLRCDRVRIWVSPNGIVT 61
          C GKM WPELLG R  EA++RIE+ENPYVD A++ EGR+VT DLRCDRVRIWV+ +GIVT
Sbjct: 5  CRGKMMWPELLGARGEEARSRIEKENPYVDAAIVREGRVVTLDLRCDRVRIWVNEDGIVT 64

Query: 62 RIPVVG 67
          R+P VG
Sbjct: 65 RVPFVG 70

BLAST of MC09g0819 vs. ExPASy TrEMBL
Match: A0A6J1JVI1 (glu S.griseus protease inhibitor-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111487932 PE=3 SV=1)

HSP 1 Score: 107 bits (266), Expect = 6.10e-29
Identity = 45/66 (68.18%), Postives = 56/66 (84.85%), Query Frame = 0

Query: 2  CAGKMAWPELLGVRRGEAKNRIERENPYVDVAVIPEGRIVTFDLRCDRVRIWVSPNGIVT 61
          C GKM WPELLG R  EA++RIE+ENPYVD A++ EGR+VT D+RCDRVR+WV+ +GIVT
Sbjct: 5  CRGKMMWPELLGARGEEARSRIEKENPYVDAAIVREGRVVTLDIRCDRVRVWVNEDGIVT 64

Query: 62 RIPVVG 67
          R+P VG
Sbjct: 65 RVPFVG 70

BLAST of MC09g0819 vs. ExPASy TrEMBL
Match: A0A6J1GMZ2 (glu S.griseus protease inhibitor-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111455981 PE=3 SV=1)

HSP 1 Score: 106 bits (265), Expect = 8.91e-29
Identity = 47/65 (72.31%), Postives = 56/65 (86.15%), Query Frame = 0

Query: 3  AGKMAWPELLGVRRGEAKNRIERENPYVDVAVIPEGRIVTFDLRCDRVRIWVSPNGIVTR 62
          AGKM WPELLG R  EA++RIE+ENPYVD A++ EGR+VT DLRCDRVRIWV+ +GIVTR
Sbjct: 7  AGKMMWPELLGARGEEARSRIEKENPYVDAAIVREGRVVTLDLRCDRVRIWVNEDGIVTR 66

Query: 63 IPVVG 67
          +P VG
Sbjct: 67 VPFVG 71

BLAST of MC09g0819 vs. ExPASy TrEMBL
Match: A0A6J1JL51 (glu S.griseus protease inhibitor-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111487932 PE=3 SV=1)

HSP 1 Score: 105 bits (262), Expect = 2.56e-28
Identity = 45/65 (69.23%), Postives = 56/65 (86.15%), Query Frame = 0

Query: 3  AGKMAWPELLGVRRGEAKNRIERENPYVDVAVIPEGRIVTFDLRCDRVRIWVSPNGIVTR 62
          AGKM WPELLG R  EA++RIE+ENPYVD A++ EGR+VT D+RCDRVR+WV+ +GIVTR
Sbjct: 7  AGKMMWPELLGARGEEARSRIEKENPYVDAAIVREGRVVTLDIRCDRVRVWVNEDGIVTR 66

Query: 63 IPVVG 67
          +P VG
Sbjct: 67 VPFVG 71

BLAST of MC09g0819 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 68.6 bits (166), Expect = 2.2e-12
Identity = 30/64 (46.88%), Postives = 42/64 (65.62%), Query Frame = 0

Query: 4  GKMAWPELLGVRRGEAKNRIERENPYVDVAVIPEGRIVTFDLRCDRVRIWVSPNGIVTRI 63
          GK +WPELLG +  +AK  IERENP +   +I +G +V     C RV +WV+  GIV +I
Sbjct: 36 GKSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVNDCGIVVQI 95

Query: 64 PVVG 68
          P++G
Sbjct: 96 PIIG 99

BLAST of MC09g0819 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 67.0 bits (162), Expect = 6.5e-12
Identity = 34/66 (51.52%), Postives = 40/66 (60.61%), Query Frame = 0

Query: 2  CAGKMAWPELLGVRRGEAKNRIERENPYVDVAVIPEGRIVTFDLRCDRVRIWVSPNGIVT 61
          C  K +WPEL G     A   IERENP V+ AVI +G  VT D RCDRVR++V  N IV 
Sbjct: 5  CPRKNSWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIVV 64

Query: 62 RIPVVG 68
          + P  G
Sbjct: 65 KTPKSG 70

BLAST of MC09g0819 vs. TAIR 10
Match: AT2G38900.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 61.6 bits (148), Expect = 2.7e-10
Identity = 30/64 (46.88%), Postives = 38/64 (59.38%), Query Frame = 0

Query: 4  GKMAWPELLGVRRGEAKNRIERENPYVDVAVIPEGRIVTFDLRCDRVRIWVSPNGIVTRI 63
          GK +WPELLG     A + I+ EN  ++V V+ +G  VT DL C RVR+WV    IV R 
Sbjct: 10 GKNSWPELLGTNGDYAASVIKGENSSLNVVVVSDGNYVTEDLSCYRVRVWVDEIRIVVRN 69

Query: 64 PVVG 68
          P  G
Sbjct: 70 PTAG 73

BLAST of MC09g0819 vs. TAIR 10
Match: AT2G38900.2 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 61.6 bits (148), Expect = 2.7e-10
Identity = 30/64 (46.88%), Postives = 38/64 (59.38%), Query Frame = 0

Query: 4  GKMAWPELLGVRRGEAKNRIERENPYVDVAVIPEGRIVTFDLRCDRVRIWVSPNGIVTRI 63
          GK +WPELLG     A + I+ EN  ++V V+ +G  VT DL C RVR+WV    IV R 
Sbjct: 25 GKNSWPELLGTNGDYAASVIKGENSSLNVVVVSDGNYVTEDLSCYRVRVWVDEIRIVVRN 84

Query: 64 PVVG 68
          P  G
Sbjct: 85 PTAG 88

BLAST of MC09g0819 vs. TAIR 10
Match: AT5G43570.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 57.8 bits (138), Expect = 4.0e-09
Identity = 28/61 (45.90%), Postives = 37/61 (60.66%), Query Frame = 0

Query: 5  KMAWPELLGVRRGEAKNRIERENPYVDVAVIPEGRIVTFDLRCDRVRIWVSPNGIVTRIP 64
          K +WPEL+G R  E K  I+REN  V   +I E  +V   + CDRV + V+  GIVTR P
Sbjct: 14 KSSWPELVGRRGEEVKEIIDRENTKVTAKIISENAVVLAVVICDRVYVRVNDQGIVTRTP 73

Query: 65 V 66
          +
Sbjct: 74 I 74

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P240761.5e-1351.52Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1[more]
P198731.7e-1250.00Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1[more]
Q007832.7e-1049.25Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1[more]
P823813.5e-1047.62Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1[more]
P162311.7e-0948.48Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KGN44697.11.31e-3075.76hypothetical protein Csa_016107 [Cucumis sativus][more]
XP_022953422.14.39e-2971.21glu S.griseus protease inhibitor-like isoform X2 [Cucurbita moschata] >XP_023547... [more]
XP_022991208.11.26e-2868.18glu S.griseus protease inhibitor-like isoform X2 [Cucurbita maxima][more]
XP_022953421.11.84e-2872.31glu S.griseus protease inhibitor-like isoform X1 [Cucurbita moschata] >XP_023547... [more]
XP_022991207.15.28e-2869.23glu S.griseus protease inhibitor-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A0A0K8J96.33e-3175.76Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G372910 PE=3 SV=1[more]
A0A6J1GN662.13e-2971.21glu S.griseus protease inhibitor-like isoform X2 OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1JVI16.10e-2968.18glu S.griseus protease inhibitor-like isoform X2 OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1GMZ28.91e-2972.31glu S.griseus protease inhibitor-like isoform X1 OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1JL512.56e-2869.23glu S.griseus protease inhibitor-like isoform X1 OS=Cucurbita maxima OX=3661 GN=... [more]
Match NameE-valueIdentityDescription
AT5G43580.12.2e-1246.88Serine protease inhibitor, potato inhibitor I-type family protein [more]
AT2G38870.16.5e-1251.52Serine protease inhibitor, potato inhibitor I-type family protein [more]
AT2G38900.12.7e-1046.88Serine protease inhibitor, potato inhibitor I-type family protein [more]
AT2G38900.22.7e-1046.88Serine protease inhibitor, potato inhibitor I-type family protein [more]
AT5G43570.14.0e-0945.90Serine protease inhibitor, potato inhibitor I-type family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000864Proteinase inhibitor I13, potato inhibitor IPRINTSPR00292POTATOINHBTRcoord: 44..54
score: 58.5
coord: 7..20
score: 50.93
coord: 32..43
score: 35.14
IPR000864Proteinase inhibitor I13, potato inhibitor IPFAMPF00280potato_inhibitcoord: 5..67
e-value: 7.4E-25
score: 87.4
IPR000864Proteinase inhibitor I13, potato inhibitor IPANTHERPTHR33091PROTEIN, PUTATIVE, EXPRESSED-RELATEDcoord: 2..67
NoneNo IPR availableGENE3D3.30.10.10Trypsin Inhibitor V, subunit Acoord: 1..66
e-value: 1.2E-26
score: 94.7
NoneNo IPR availablePANTHERPTHR33091:SF41SERINE PROTEASE INHIBITOR, POTATO INHIBITOR I-TYPE FAMILY PROTEIN-RELATEDcoord: 2..67
IPR036354Proteinase inhibitor I13, potato inhibitor I superfamilySUPERFAMILY54654CI-2 family of serine protease inhibitorscoord: 2..67

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g0819.1MC09g0819.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
biological_process GO:0009611 response to wounding
molecular_function GO:0004867 serine-type endopeptidase inhibitor activity