MC09g0804 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g0804
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionacyl-coenzyme A thioesterase 9, mitochondrial-like
LocationMC09: 8105710 .. 8110936 (-)
RNA-Seq ExpressionMC09g0804
SyntenyMC09g0804
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
TGCAAGTTTAACTAAAAAATAATAATTAGTAACAATAATACCTCAATTATTTGAGGTAAAAGGTAGATTCCAAAAGATCTAAAGAACTTCATAAATTAGTTTACAGATGGGACCTGGCAGCCTGTTCCTGTGGGGAAGGAAAGTCCAAATTCTTCGATCTCTGAACTCTGCGTTGCCCTCGAATGTTGGAGCAGCTGTTGTTGTAGCCCACAAGAAATCAGCCATGGATTTCAGTTCTTCTCCTTCCAAGACCATTTCCGTTTTCTCCACCATTCCTCTGCCGCCGCCGGCCGCCGACGCCGTCGATGGCTCCGGCAATCCCGCTTCGTCGCGGAAACCCATCAGCCTCTGGCCGGGTATGCACCATTCGCCGGTGACGACCGCTCTTTGGGAAGCCAGGTCCAGCATCTTCGAAAGGCTGCTCGATCCCCCCAAAGACGCGCCGCCGCAGAGCGAACTGCTCACCAAGACGCCCTCCCAGAGTCGGACCATCGTTATCTACAAATTCTCTTCTGATTTCATTCTCAGGGAGCAGTATCGAGATCCATGGAACGAGGTCAGAATCGGAAAGCTGCTTGAAGATCTTGATGCCTTGGCTGGCACCATCTCCGTCAGAGTATGCCGCCTCCCCTTCGTTTCATCCTTTTCAAATTGATAGCGGCTTTTTAAATGCAATTTCCTAACCCTAGCTCTTTACTAGCGCTAATTCTGCTGTTTTTCCCATTTTGTAATCTTTATTTCCGCGACGATTTGAAGAGGATTTGAACTTCTTTATAATCTCTCAAGCTCTCTTCCGTTATGATTTCTCACATTTAATAGAAATTAAAGAATAAAAGTAGCTTAAGCGTTTCTTCTTTAGTTTGAATTTTGCATTTCTTTTTTAGTTTTTAACTTCGAAGAGTTTAAGTTCAAAGTTAGCTGAATATGAATTGGTAAAGGGTTTCTGCATCTTATGAATTTATCAGATTCTCTTCCTTCTTAAGATTTTGTTTTGTTTCCTCTTTGACAGCACTGTTCTGATGTTGATAGCACTACACGGCCACTTTTGGTGGTAACTGCATCTGTCGACAGGATTGTTTTGAAGAAGTCAATTAGTGTTGATGCTGATCTGAAAATAGTTGGTTCAGTCATATGGGTTGGACGATCATCAATTGAGGTTCAGTTAGACATGATCCAGTCTACAGAAGGTATGGCCCTTAGGAGTTAGGAATATAGTTTAATGAAGTGGTGAATCAATGCGACGACCATTCTATCTGTTATAAAATTGAAGTTCCAAAATCTCTTCTTTCTCTCTCTCTCTGATTTTTTTTTAGAAATGAACTTCAAATTTTCACTGGGTTCTTGGGCGAATTCCATTATGAAAATTCTCTGCCTCAATTTGGCCTCTGTCTAACAATAATGTACTGTAAACTTTGAGAGTATGATTGAAACACTAAAAGAATGGCCATTTTGTTGATATTTCTGTCTCTTATAAAAAATAAAAACGCTAAAAGAATGGCAAAGTGAACAAATCTTCGGAAATTGTAACACTTGGATCTTAGTATATTTGGGGTTTCTTCTGTGTTTTTCATTCATCTTTTGACATCAAAAGTTTTAAATATGAAGTATGCTTTTGACATCCACTGTCTTCCAAAATTTTAAATTAAAATTATGGAACTTACTTTTTTCTTAAACGTATTTTATCGTTAATGTAACGATGATTACTGCAATTTTTTATATTTCAGGACAATAGAACCTAGATCAATTATATTAGAATTAAATACCTCTCCCCATCGACAAACTTATTGAACTCTATTTCTTTATTTTCCTATTAATAATTTTGGGTGTCAATATCATTTGTAGGATACTAATATCCGGAGAAGATGACCACAAAGACTTGGATCTATGTATAATAGACATTGAGATTTACAACTTATCTTCTTACCTCTAGACCAACTCATTATGATTATTGAACTTTGTTTCCTATAGACAAAAAAATACAATAACCACATGGAAAGAAAGATGGGAACAAAAATCCTAGGTTTATAAGCATGGAAGCAGAAGAATTCAGATGAGAGAGAGTAAGGGAGCAACAGAGAGAGTAAGAGAGGCTTATAGAAGAAGACTCTTTGAGTTTGTATGGAGGTATTTTGGTAGAAAGGTCAAGAGAGTTGAGAAAAGTTTTTCTTGAATTTATTCGTTAGAACTTGATCCTGGTGCTATGGGTTAGTGATGGTTTAGTGCGATGGGTTAGTGTTGGTGTTGTCCAAAAAGTTAAAGAACTCCTTGTATTATCCTTAAAAATTTTAATGATGCTCTCAAATTCTCAATTCAAAGAGACCAAAGAAGGGTTATTGGGACATTTCTCCATACAACTCTAGCTTTTCCATAGAGTCACCAACTTGATAAAACTTCAGGAAGCAGAAGATTTGTATCTATTAGACAGCACCAAGCTAAACCAAACTTGTGAAAGAGAATTGCAGCTTCTTCTAACAAAATTGCAATGACTAAACAAATGGTCTAGAGTTGCCCACCCAGCATCTTGGGTGTTTTCACTTTGGTAAGATACAATTCTGTTGGGACTTTTCCTGTCCAAATCAACTTAATTTGATGTCCTTCCAGCTTGCTAATAGTGGCATTAGTTGGAGTGCTGCTGATTTACATGATTCAACTCCAATACTTACGATTGGAATTTTGATTAAAATTTGAAACCTTCTAGCCTCATGGGGAGTTCTGTTGTTGTGACTGCTCTCTATCAGACATTCAAACTCCATCCCTGCATTCTTGAAGAGTCTTTCCGTTGCCCCTACTGATGCAAGGAGAATAACTGACATAAAAGGCAGCAGTCGAGCCATCTGTCTTCCTAAAATCTTTTTTTTTTCCCTTGAGTACGACTCCTAAAATCTTATTCTGTCTCTATTACATTACCAGCCTGAATCAGGTTCATTTCTCCATCACACTCCAGCATATCTATGTATAGAAGAATGCATCCAAAAATCCAACTCCATTCGGACCTGTATTTCCATTGTTATTTCATGATATTTATTTTATTATTTTATTATTTTATGAGAAACAGATCAGAAGTTTCATTCAGAAAGCCAACTGGGTGAAGACAGCCTAGCAGACAAAGGGATAAGGCAACCGACTTTATTGTAGACTTTTCCATAATCTACCATAATAAAGCATTTTCATTTTCTTCAGGTTCCCACATCTATGACTTGATCTTTCTGAAAGATGTCTATACTACTGACTACTGACTACTATTGTCAAACAATCTTGAATGTCCGCAACCGCTTCTAAAACCAAGCGAAACTGGAGACTCTTAACTACTCTTAAACAAGAAAATTGTAAAAAAACCTTCTTCCAAAGAGCAACAAAAGGTTATTTTATCACCATGGTAGATTTTGAGAATCTCTTTTTTCTGCTAAAATGAAACTTGTGCAGAACGTTAAATGGATTAAGGAAATTTTTTATGTTTTGACAAGAATTCACGCAGATTTTGCGTTGGACATCCTTTTGCCTGGTTTCTGGGTCCAATCACCTAGCTATTGACTGCTAAATTGTTGAGTAATGGTGTTTCTTATCATAAATCAAACTTTTTGTTCTTTTTGCAGAATGCTCAAACACCTCAGATGCAGTGGCTCTGACAGCGAACTTCATATTTGTGGCTCGTGACTCCCAGACTGGGAAAGCTGCTTTGGTTAACAGGCTTTTGCCAGAAACTGAGGAAGAAAAATTACTTTACGAGGGAGCGGAAGCCAGAAGTAAATTAAGGAAAAGGAAGAACAGAGGTGATATGTGGGAGTTAGAAAATGGGGACATAAATAGACTTGATGCCTTGTTAGCAGAGGGAAGAATCTTCTGTGACATGCCAGCCTTGGCAGACAGAGACAGCATTCTTCTCAGGGATACTGGCCTTGAGAACTCATTGATTTGTCAACCACAACAAAGAAATATACATGGTCGGATTTTCGGGGGGTTTCTGATGCAACGGGCATTCGAATTGGCTTTCTCAACAGCTTATGTATTTGCTGGAATGGTGCCTTACTTTCTAGAAGTTGATCATGTTGATTTCTTAAAACCTGTGAGTGCACTTCTCTTTTCTACTCTAACATAGGAATATGGTTACTTCCTTGGTGTAATCTTTGCCTTTTCCATTGGAAAGGAAATAGATATTCCAATTGCATAAATGTAGGCTGTTCCAATGTGCAGGTGGACGTTGGAGACTTTCTACGCTTCAAATCATGCGTTCTTTACACGGAAGTCGAAAATCCAGATCAGCCATTGATCAATGTTGAAGTTGTTGCCCATGTTACGAGGCCTGAGATTAGGTCTAGCGAGGTAAAGTTCCCGTGTATGCGTATGGCTATACTATAAATCTATGGGTTTAGGGCTTTAGAGTTTTTTTTTATTTATTTATTTATACTACTATTTGTAGTTCTTAAAATTTTGTTTAGATTTTAAAAATCTTTTAAAAAGAGTAGATAACACAGCAAAAACACATACTTAAATTTTCAGTTGCAAAAAATAAAAAACAAAATGGTTATCAGACTGGGTTTAGTAATATAAAGACTACATTTGAATAAGTATAAAAGTCTCCGATGAAATATTATTTAAACTTTTAAATCCAATTATGTTCATAGCCTATTATTCAAACTGGAAAGAACTAGAGAGCGTAAAGTATTCGATTCGACGGAGGTTTTAAAATTCCCCCACCCTCACAATTGCTTTTGTCAAAAATGGAAATTTACGATAAAAAATGAAGTCAAATGTGTTTCTAATGTTAGTAGTTTATGTTAATCATTGGCAATCAATCTCTAGCAACTTGTTAACTTGCTAATTGGTTGTGAATCTGTGATTTACTTGCCAAATGCAGGTTTCAAACACATTCTACTTCACTTTTACCGTGCGCCCTGAGGCAAAAGCAAACCGAAATGGGTTCAGACTTCGCAACGTCGTTCCAGCAACAGAAGAAGAAGCTCGCCGTATTATCGAGCGCATGGATGCCGAAAAGCTGCTGAGGGACTTGAGCAAGCAATCTGAATCAGGTCAGATGTAGACAGCAAACACAGTTTATCTTAGAAAATTATTGTAGAATATTTGTGAAGAAAATTAATCTTATTCAATAGGTGTGAGTATGGCTGAAGAAAAGTAAATCAAATTATGTATTGGAGTACTGATGGTTTCACTTTAGTGATAAATATCTCATCTTATTGGAGCTCAAATTCAGAAGTTTCTCGAATGGTTT

mRNA sequence

TGCAAGTTTAACTAAAAAATAATAATTAGTAACAATAATACCTCAATTATTTGAGGTAAAAGGTAGATTCCAAAAGATCTAAAGAACTTCATAAATTAGTTTACAGATGGGACCTGGCAGCCTGTTCCTGTGGGGAAGGAAAGTCCAAATTCTTCGATCTCTGAACTCTGCGTTGCCCTCGAATGTTGGAGCAGCTGTTGTTGTAGCCCACAAGAAATCAGCCATGGATTTCAGTTCTTCTCCTTCCAAGACCATTTCCGTTTTCTCCACCATTCCTCTGCCGCCGCCGGCCGCCGACGCCGTCGATGGCTCCGGCAATCCCGCTTCGTCGCGGAAACCCATCAGCCTCTGGCCGGGTATGCACCATTCGCCGGTGACGACCGCTCTTTGGGAAGCCAGGTCCAGCATCTTCGAAAGGCTGCTCGATCCCCCCAAAGACGCGCCGCCGCAGAGCGAACTGCTCACCAAGACGCCCTCCCAGAGTCGGACCATCGTTATCTACAAATTCTCTTCTGATTTCATTCTCAGGGAGCAGTATCGAGATCCATGGAACGAGGTCAGAATCGGAAAGCTGCTTGAAGATCTTGATGCCTTGGCTGGCACCATCTCCGTCAGACACTGTTCTGATGTTGATAGCACTACACGGCCACTTTTGGTGGTAACTGCATCTGTCGACAGGATTGTTTTGAAGAAGTCAATTAGTGTTGATGCTGATCTGAAAATAGTTGGTTCAGTCATATGGGTTGGACGATCATCAATTGAGGTTCAGTTAGACATGATCCAGTCTACAGAAGAATGCTCAAACACCTCAGATGCAGTGGCTCTGACAGCGAACTTCATATTTGTGGCTCGTGACTCCCAGACTGGGAAAGCTGCTTTGGTTAACAGGCTTTTGCCAGAAACTGAGGAAGAAAAATTACTTTACGAGGGAGCGGAAGCCAGAAGTAAATTAAGGAAAAGGAAGAACAGAGGTGATATGTGGGAGTTAGAAAATGGGGACATAAATAGACTTGATGCCTTGTTAGCAGAGGGAAGAATCTTCTGTGACATGCCAGCCTTGGCAGACAGAGACAGCATTCTTCTCAGGGATACTGGCCTTGAGAACTCATTGATTTGTCAACCACAACAAAGAAATATACATGGTCGGATTTTCGGGGGGTTTCTGATGCAACGGGCATTCGAATTGGCTTTCTCAACAGCTTATGTATTTGCTGGAATGGTGCCTTACTTTCTAGAAGTTGATCATGTTGATTTCTTAAAACCTGTGGACGTTGGAGACTTTCTACGCTTCAAATCATGCGTTCTTTACACGGAAGTCGAAAATCCAGATCAGCCATTGATCAATGTTGAAGTTGTTGCCCATGTTACGAGGCCTGAGATTAGGTCTAGCGAGGTTTCAAACACATTCTACTTCACTTTTACCGTGCGCCCTGAGGCAAAAGCAAACCGAAATGGGTTCAGACTTCGCAACGTCGTTCCAGCAACAGAAGAAGAAGCTCGCCGTATTATCGAGCGCATGGATGCCGAAAAGCTGCTGAGGGACTTGAGCAAGCAATCTGAATCAGGTCAGATGTAGACAGCAAACACAGTTTATCTTAGAAAATTATTGTAGAATATTTGTGAAGAAAATTAATCTTATTCAATAGGTGTGAGTATGGCTGAAGAAAAGTAAATCAAATTATGTATTGGAGTACTGATGGTTTCACTTTAGTGATAAATATCTCATCTTATTGGAGCTCAAATTCAGAAGTTTCTCGAATGGTTT

Coding sequence (CDS)

ATGGGACCTGGCAGCCTGTTCCTGTGGGGAAGGAAAGTCCAAATTCTTCGATCTCTGAACTCTGCGTTGCCCTCGAATGTTGGAGCAGCTGTTGTTGTAGCCCACAAGAAATCAGCCATGGATTTCAGTTCTTCTCCTTCCAAGACCATTTCCGTTTTCTCCACCATTCCTCTGCCGCCGCCGGCCGCCGACGCCGTCGATGGCTCCGGCAATCCCGCTTCGTCGCGGAAACCCATCAGCCTCTGGCCGGGTATGCACCATTCGCCGGTGACGACCGCTCTTTGGGAAGCCAGGTCCAGCATCTTCGAAAGGCTGCTCGATCCCCCCAAAGACGCGCCGCCGCAGAGCGAACTGCTCACCAAGACGCCCTCCCAGAGTCGGACCATCGTTATCTACAAATTCTCTTCTGATTTCATTCTCAGGGAGCAGTATCGAGATCCATGGAACGAGGTCAGAATCGGAAAGCTGCTTGAAGATCTTGATGCCTTGGCTGGCACCATCTCCGTCAGACACTGTTCTGATGTTGATAGCACTACACGGCCACTTTTGGTGGTAACTGCATCTGTCGACAGGATTGTTTTGAAGAAGTCAATTAGTGTTGATGCTGATCTGAAAATAGTTGGTTCAGTCATATGGGTTGGACGATCATCAATTGAGGTTCAGTTAGACATGATCCAGTCTACAGAAGAATGCTCAAACACCTCAGATGCAGTGGCTCTGACAGCGAACTTCATATTTGTGGCTCGTGACTCCCAGACTGGGAAAGCTGCTTTGGTTAACAGGCTTTTGCCAGAAACTGAGGAAGAAAAATTACTTTACGAGGGAGCGGAAGCCAGAAGTAAATTAAGGAAAAGGAAGAACAGAGGTGATATGTGGGAGTTAGAAAATGGGGACATAAATAGACTTGATGCCTTGTTAGCAGAGGGAAGAATCTTCTGTGACATGCCAGCCTTGGCAGACAGAGACAGCATTCTTCTCAGGGATACTGGCCTTGAGAACTCATTGATTTGTCAACCACAACAAAGAAATATACATGGTCGGATTTTCGGGGGGTTTCTGATGCAACGGGCATTCGAATTGGCTTTCTCAACAGCTTATGTATTTGCTGGAATGGTGCCTTACTTTCTAGAAGTTGATCATGTTGATTTCTTAAAACCTGTGGACGTTGGAGACTTTCTACGCTTCAAATCATGCGTTCTTTACACGGAAGTCGAAAATCCAGATCAGCCATTGATCAATGTTGAAGTTGTTGCCCATGTTACGAGGCCTGAGATTAGGTCTAGCGAGGTTTCAAACACATTCTACTTCACTTTTACCGTGCGCCCTGAGGCAAAAGCAAACCGAAATGGGTTCAGACTTCGCAACGTCGTTCCAGCAACAGAAGAAGAAGCTCGCCGTATTATCGAGCGCATGGATGCCGAAAAGCTGCTGAGGGACTTGAGCAAGCAATCTGAATCAGGTCAGATGTAG

Protein sequence

MGPGSLFLWGRKVQILRSLNSALPSNVGAAVVVAHKKSAMDFSSSPSKTISVFSTIPLPPPAADAVDGSGNPASSRKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPPQSELLTKTPSQSRTIVIYKFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRHCSDVDSTTRPLLVVTASVDRIVLKKSISVDADLKIVGSVIWVGRSSIEVQLDMIQSTEECSNTSDAVALTANFIFVARDSQTGKAALVNRLLPETEEEKLLYEGAEARSKLRKRKNRGDMWELENGDINRLDALLAEGRIFCDMPALADRDSILLRDTGLENSLICQPQQRNIHGRIFGGFLMQRAFELAFSTAYVFAGMVPYFLEVDHVDFLKPVDVGDFLRFKSCVLYTEVENPDQPLINVEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKANRNGFRLRNVVPATEEEARRIIERMDAEKLLRDLSKQSESGQM
Homology
BLAST of MC09g0804 vs. ExPASy Swiss-Prot
Match: S4TDL2 (Acyl-coenzyme A thioesterase 2, chloroplastic OS=Humulus lupulus OX=3486 GN=TE2 PE=2 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 3.7e-194
Identity = 349/437 (79.86%), Postives = 386/437 (88.33%), Query Frame = 0

Query: 40  MDFSSSPSKTISVFSTIPLPPPAADAVDGSGNPASSRKPISLWPGMHHSPVTTALWEARS 99
           MD SSSP+  I+V ST   P     +V  S   +S+RKPISLWPGM+HSPVT ALWEAR 
Sbjct: 1   MDLSSSPNHPITVVSTFASPFEGPPSVGDSN--SSARKPISLWPGMYHSPVTNALWEARC 60

Query: 100 SIFERLLDPPKDAPPQSELLTKTPSQSRTIVIYKFSSDFILREQYRDPWNEVRIGKLLED 159
            IFERLLDPPKDAPPQSELLTKTPSQSRT ++Y FS+D+ILREQYRDPWNEVRIGKLLED
Sbjct: 61  KIFERLLDPPKDAPPQSELLTKTPSQSRTTILYNFSTDYILREQYRDPWNEVRIGKLLED 120

Query: 160 LDALAGTISVRHCSDVDSTTRPLLVVTASVDRIVLKKSISVDADLKIVGSVIWVGRSSIE 219
           LDALAGTISV+HCSD DSTTRPLL+VTASVD+IVLKK ISVD DLKIVG+VIWVGRSSIE
Sbjct: 121 LDALAGTISVKHCSDDDSTTRPLLLVTASVDKIVLKKPISVDIDLKIVGAVIWVGRSSIE 180

Query: 220 VQLDMIQSTEECSNTSDAVALTANFIFVARDSQTGKAALVNRLLPETEEEKLLYEGAEAR 279
           +QL++ QST+E SN +D VAL+ANFIFVARDS+T KAA VNRL PETE+EKLL++ AEAR
Sbjct: 181 IQLEVSQSTKEGSNAADDVALSANFIFVARDSKTAKAAPVNRLSPETEQEKLLFDEAEAR 240

Query: 280 SKLRKRKNRGDM--WELENGDINRLDALLAEGRIFCDMPALADRDSILLRDTGLENSLIC 339
           S +RKRK RGD    E ENG+ NRL  LLAEGRIFCDMPALADRDSILLRDT  ENSLIC
Sbjct: 241 SSMRKRK-RGDQERREFENGEANRLQTLLAEGRIFCDMPALADRDSILLRDTRQENSLIC 300

Query: 340 QPQQRNIHGRIFGGFLMQRAFELAFSTAYVFAGMVPYFLEVDHVDFLKPVDVGDFLRFKS 399
           QPQQRNIHGRIFGGFL+ RAFELAFSTAY FAG+VPYFLE+DHVDF +PVDVGDFLR KS
Sbjct: 301 QPQQRNIHGRIFGGFLLHRAFELAFSTAYAFAGLVPYFLEIDHVDFXRPVDVGDFLRLKS 360

Query: 400 CVLYTEVENPDQPLINVEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKANRNGFRLRNVV 459
           CVLYTE+ NPDQPLIN+EVVAHVTRPE+RSSEVSNTFYFTFTVRPEAKA +NG+R+RNVV
Sbjct: 361 CVLYTELHNPDQPLINIEVVAHVTRPELRSSEVSNTFYFTFTVRPEAKATKNGYRIRNVV 420

Query: 460 PATEEEARRIIERMDAE 475
           PATEEEARRI+ERMDAE
Sbjct: 421 PATEEEARRILERMDAE 434

BLAST of MC09g0804 vs. ExPASy Swiss-Prot
Match: S4TF94 (Acyl-coenzyme A thioesterase 4, mitochondrial OS=Humulus lupulus OX=3486 GN=TE4 PE=1 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 1.9e-158
Identity = 291/439 (66.29%), Postives = 360/439 (82.00%), Query Frame = 0

Query: 38  SAMDFSSSPSKTISVFSTIPLPPPAADAVDGSGNPASSRKPISLWPGMHHSPVTTALWEA 97
           S++DF   PS    V STI      +  +D     +S RKPISLWPGM +SPVT ALWEA
Sbjct: 86  SSLDF---PSNQPPVVSTISSHHNISQPIDAG---SSIRKPISLWPGMFNSPVTNALWEA 145

Query: 98  RSSIFERLLDPPKDAPPQSELLTKTPSQSRTIVIYKFSSDFILREQYRDPWNEVRIGKLL 157
           RS++FE+  +P  D P QSEL+TK+P+QSRT ++Y  SSD+ LRE YR+PWN +RIGKLL
Sbjct: 146 RSNMFEKYGEPTADPPSQSELVTKSPAQSRTSILYNLSSDYALREHYRNPWNMIRIGKLL 205

Query: 158 EDLDALAGTISVRHCSDVDSTTRPLLVVTASVDRIVLKKSISVDADLKIVGSVIWVGRSS 217
           EDLDALAGTI+ +HC++ D  +RPLL+VTASVD++VLKK IS+D DL IVG+V WVGRSS
Sbjct: 206 EDLDALAGTIAFKHCTNEDGMSRPLLLVTASVDKMVLKKPISIDTDLSIVGAVTWVGRSS 265

Query: 218 IEVQLDMIQSTEECSNTSDAVALTANFIFVARDSQTGKAALVNRLLPETEEEKLLYEGAE 277
           +E+QL ++Q+T E S+ SD+V+L ANF FVARDS+TGK+A++N++ PET EEKLL+  A+
Sbjct: 266 MEIQLQVLQTTHESSDPSDSVSLVANFTFVARDSKTGKSAVINQISPETGEEKLLWREAD 325

Query: 278 ARSKLR--KRKNRGDMWELENGDINRLDALLAEGRIFCDMPALADRDSILLRDTGLENSL 337
            R+K+R  KRK + D+ ELE   I RL+ALLAEGR+FCD+PALADR+SIL++DT LENS 
Sbjct: 326 ERNKMRKMKRKIQKDL-ELEKQYIERLNALLAEGRVFCDLPALADRNSILMKDTCLENSF 385

Query: 338 ICQPQQRNIHGRIFGGFLMQRAFELAFSTAYVFAGMVPYFLEVDHVDFLKPVDVGDFLRF 397
           ICQPQQRNI+GRIFGGFLM+RA ELAFST Y FAG+V +FLEVDHVDFL+PVDVGDFLR 
Sbjct: 386 ICQPQQRNIYGRIFGGFLMRRAVELAFSTTYSFAGVVTHFLEVDHVDFLRPVDVGDFLRL 445

Query: 398 KSCVLYTEVENPDQPLINVEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKANRNGFRLRN 457
           KSCVLYTE++NP +PLINVEVVAHVTRPE+RSSEVSN FYFTF+V PEA   ++G  +RN
Sbjct: 446 KSCVLYTELQNPTEPLINVEVVAHVTRPELRSSEVSNKFYFTFSVGPEAV--KDGLLVRN 505

Query: 458 VVPATEEEARRIIERMDAE 475
           VVPATEEEARR++ERMDAE
Sbjct: 506 VVPATEEEARRVLERMDAE 515

BLAST of MC09g0804 vs. ExPASy Swiss-Prot
Match: Q9R0X4 (Acyl-coenzyme A thioesterase 9, mitochondrial OS=Mus musculus OX=10090 GN=Acot9 PE=1 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 2.1e-40
Identity = 110/320 (34.38%), Postives = 169/320 (52.81%), Query Frame = 0

Query: 130 VIYKFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRHCSDVDSTTRPLLVVTASV 189
           V+    +D  LR++Y    N VR G++LEDLD+L   +   H  +  +   PL +VT  V
Sbjct: 89  VLLPLGTDPELRDKYVTVQNTVRFGRILEDLDSLGVLVCYMHNHNHSTKMSPLSIVTVLV 148

Query: 190 DRI-VLKKSISVDADLKIVGSVIWVGRSSIEVQLDMIQSTEECSNTSDAVALTANFIFVA 249
           D+I + K S+S + D+K  G V WVG +S+EV++ M Q     ++      L A F+ VA
Sbjct: 149 DKIDMCKHSLSPEQDIKFTGHVSWVGNTSMEVKMKMFQLH---NDEKYWPVLDATFVMVA 208

Query: 250 RDSQTGKAALVNRLLPETEEEKLLYEGAEARSKLRKRKNRGDMWEL-----ENGDINRLD 309
           RDS+    A VN L+PE +EE+ L++  E     R   +   + ++     E   I+ L 
Sbjct: 209 RDSENKGPAFVNPLIPENKEEEELFKQGELNKSRRIAFSTSSLLKVAPSSEERNIIHELF 268

Query: 310 ALLAEGRIFCDMPALADRDSILLRDTGLENSLICQPQQRNIHGRIFGGFLMQRAFELAFS 369
               + +       +    ++ + DT L++  IC PQ+RN+  RIFGGFLM++A+ELA++
Sbjct: 269 LTTLDPKTISFQSRILPPKAVWMEDTKLKSLDICHPQERNVFNRIFGGFLMRKAYELAWA 328

Query: 370 TAYVFAGMVPYFLEVDHVDFLKPVDVGDFLRFKSCVLYTEVENPDQPLINVEVVAHVTRP 429
           TA  F G  PY + VD + F KPV+VG  L   S V +T+        I V V + V+  
Sbjct: 329 TACSFGGSRPYVVTVDDIMFQKPVEVGSLLFLSSQVCFTQ-----DNYIQVRVHSEVSSL 388

Query: 430 EIRSSEVSNTFYFTFTVRPE 444
           + R    +N F+FTF    E
Sbjct: 389 DSREHMTTNVFHFTFMSEKE 400

BLAST of MC09g0804 vs. ExPASy Swiss-Prot
Match: Q9Y305 (Acyl-coenzyme A thioesterase 9, mitochondrial OS=Homo sapiens OX=9606 GN=ACOT9 PE=1 SV=2)

HSP 1 Score: 167.2 bits (422), Expect = 4.8e-40
Identity = 116/340 (34.12%), Postives = 175/340 (51.47%), Query Frame = 0

Query: 112 APPQSELLTKTPSQSRTIVIYKFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRH 171
           A  Q  L  +    S   V+    S+  LRE+Y    N VR G++LEDLD+L   I   H
Sbjct: 72  AKSQDGLPPRRMKDSYIEVLLPLGSEPELREKYLTVQNTVRFGRILEDLDSLGVLICYMH 131

Query: 172 CSDVDSTTRPLLVVTASVDRI-VLKKSISVDADLKIVGSVIWVGRSSIEVQLDMIQ--ST 231
                +   PL +VTA VD+I + KKS+S + D+K  G V WVG++S+EV++ M Q    
Sbjct: 132 NKIHSAKMSPLSIVTALVDKIDMCKKSLSPEQDIKFSGHVSWVGKTSMEVKMQMFQLHGD 191

Query: 232 EECSNTSDAVALTANFIFVARDSQTGKAALVNRLLPETEEEKLLYEGAEARSKLRKRKNR 291
           E C        L A F+ VARDS+    A VN L+PE+ EE+ L+   E     R   + 
Sbjct: 192 EFCP------VLDATFVMVARDSENKGPAFVNPLIPESPEEEELFRQGELNKGRRIAFSS 251

Query: 292 GDMWEL-----ENGDINRLDALLAEGRIFCDMPALADRDSILLRDTGLENSLICQPQQRN 351
             + ++     E   I+ +     + +       +   +++ + ++ L++  IC PQ+RN
Sbjct: 252 TSLLKMAPSAEERTTIHEMFLSTLDPKTISFRSRVLPSNAVWMENSKLKSLEICHPQERN 311

Query: 352 IHGRIFGGFLMQRAFELAFSTAYVFAGMVPYFLEVDHVDFLKPVDVGDFLRFKSCVLYTE 411
           I  RIFGGFLM++A+ELA++TA  F G  P+ + VD + F KPV+VG  L   S V +T+
Sbjct: 312 IFNRIFGGFLMRKAYELAWATACSFGGSRPFVVAVDDIMFQKPVEVGSLLFLSSQVCFTQ 371

Query: 412 VENPDQPLINVEVVAHVTRPEIRSSEVSNTFYFTFTVRPE 444
                   I V V + V   + +    +N F+FTF    E
Sbjct: 372 -----NNYIQVRVHSEVASLQEKQHTTTNVFHFTFMSEKE 400

BLAST of MC09g0804 vs. ExPASy Swiss-Prot
Match: Q32MW3 (Acyl-coenzyme A thioesterase 10, mitochondrial OS=Mus musculus OX=10090 GN=Acot10 PE=2 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 1.5e-38
Identity = 107/320 (33.44%), Postives = 168/320 (52.50%), Query Frame = 0

Query: 130 VIYKFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRHCSDVDSTTRPLLVVTASV 189
           V+    +D  LR++Y    N VR G++LEDLD+L   +   H  +  +    L +VT  V
Sbjct: 89  VLLPLGTDPELRDKYVTVQNTVRFGRILEDLDSLGVLVCYMHNHNHSTNMSLLSIVTVLV 148

Query: 190 DRI-VLKKSISVDADLKIVGSVIWVGRSSIEVQLDMIQSTEECSNTSDAVALTANFIFVA 249
           D+I + K S+S + D+K  G V WVG +++EV++ M Q  ++    +    L A F+ VA
Sbjct: 149 DKIDMCKHSLSPEQDIKFTGHVSWVGNTTMEVKMKMFQLHDD---ETYWPVLDATFVMVA 208

Query: 250 RDSQTGKAALVNRLLPETEEEKLLYEGAEARSKLRKRKNRGDMWEL-----ENGDINRLD 309
           +DS+  + A VN L+PE +EE+ L+   E     R   +   + ++     E   I+ L 
Sbjct: 209 QDSENKRPAFVNPLIPENKEEEELFTQGELNKSRRIAFSTSSLLKVAPSSEERNIIHELF 268

Query: 310 ALLAEGRIFCDMPALADRDSILLRDTGLENSLICQPQQRNIHGRIFGGFLMQRAFELAFS 369
               + +       +    ++ + DT L++  IC PQ+RN+  RIFGGFLM++A+ELA++
Sbjct: 269 LSTLDPKTISFQSRILPPKAVWMEDTKLKSLDICHPQERNVFNRIFGGFLMRKAYELAWA 328

Query: 370 TAYVFAGMVPYFLEVDHVDFLKPVDVGDFLRFKSCVLYTEVENPDQPLINVEVVAHVTRP 429
           TA  F G  PY + VD + F KPV+VG  L   S V +T+        I V V + V   
Sbjct: 329 TACSFGGSRPYVVTVDDIMFQKPVEVGSLLFLSSQVCFTQGN-----YIQVRVHSEVFSL 388

Query: 430 EIRSSEVSNTFYFTFTVRPE 444
           + R    +N F+FTF    E
Sbjct: 389 DSREHMTTNVFHFTFMSEKE 400

BLAST of MC09g0804 vs. NCBI nr
Match: XP_022143585.1 (acyl-coenzyme A thioesterase 9, mitochondrial-like [Momordica charantia])

HSP 1 Score: 945 bits (2443), Expect = 0.0
Identity = 489/489 (100.00%), Postives = 489/489 (100.00%), Query Frame = 0

Query: 1   MGPGSLFLWGRKVQILRSLNSALPSNVGAAVVVAHKKSAMDFSSSPSKTISVFSTIPLPP 60
           MGPGSLFLWGRKVQILRSLNSALPSNVGAAVVVAHKKSAMDFSSSPSKTISVFSTIPLPP
Sbjct: 1   MGPGSLFLWGRKVQILRSLNSALPSNVGAAVVVAHKKSAMDFSSSPSKTISVFSTIPLPP 60

Query: 61  PAADAVDGSGNPASSRKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPPQSELLT 120
           PAADAVDGSGNPASSRKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPPQSELLT
Sbjct: 61  PAADAVDGSGNPASSRKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPPQSELLT 120

Query: 121 KTPSQSRTIVIYKFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRHCSDVDSTTR 180
           KTPSQSRTIVIYKFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRHCSDVDSTTR
Sbjct: 121 KTPSQSRTIVIYKFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRHCSDVDSTTR 180

Query: 181 PLLVVTASVDRIVLKKSISVDADLKIVGSVIWVGRSSIEVQLDMIQSTEECSNTSDAVAL 240
           PLLVVTASVDRIVLKKSISVDADLKIVGSVIWVGRSSIEVQLDMIQSTEECSNTSDAVAL
Sbjct: 181 PLLVVTASVDRIVLKKSISVDADLKIVGSVIWVGRSSIEVQLDMIQSTEECSNTSDAVAL 240

Query: 241 TANFIFVARDSQTGKAALVNRLLPETEEEKLLYEGAEARSKLRKRKNRGDMWELENGDIN 300
           TANFIFVARDSQTGKAALVNRLLPETEEEKLLYEGAEARSKLRKRKNRGDMWELENGDIN
Sbjct: 241 TANFIFVARDSQTGKAALVNRLLPETEEEKLLYEGAEARSKLRKRKNRGDMWELENGDIN 300

Query: 301 RLDALLAEGRIFCDMPALADRDSILLRDTGLENSLICQPQQRNIHGRIFGGFLMQRAFEL 360
           RLDALLAEGRIFCDMPALADRDSILLRDTGLENSLICQPQQRNIHGRIFGGFLMQRAFEL
Sbjct: 301 RLDALLAEGRIFCDMPALADRDSILLRDTGLENSLICQPQQRNIHGRIFGGFLMQRAFEL 360

Query: 361 AFSTAYVFAGMVPYFLEVDHVDFLKPVDVGDFLRFKSCVLYTEVENPDQPLINVEVVAHV 420
           AFSTAYVFAGMVPYFLEVDHVDFLKPVDVGDFLRFKSCVLYTEVENPDQPLINVEVVAHV
Sbjct: 361 AFSTAYVFAGMVPYFLEVDHVDFLKPVDVGDFLRFKSCVLYTEVENPDQPLINVEVVAHV 420

Query: 421 TRPEIRSSEVSNTFYFTFTVRPEAKANRNGFRLRNVVPATEEEARRIIERMDAEKLLRDL 480
           TRPEIRSSEVSNTFYFTFTVRPEAKANRNGFRLRNVVPATEEEARRIIERMDAEKLLRDL
Sbjct: 421 TRPEIRSSEVSNTFYFTFTVRPEAKANRNGFRLRNVVPATEEEARRIIERMDAEKLLRDL 480

Query: 481 SKQSESGQM 489
           SKQSESGQM
Sbjct: 481 SKQSESGQM 489

BLAST of MC09g0804 vs. NCBI nr
Match: XP_038899833.1 (LOW QUALITY PROTEIN: acyl-coenzyme A thioesterase 9, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 797 bits (2058), Expect = 8.09e-288
Identity = 414/446 (92.83%), Postives = 427/446 (95.74%), Query Frame = 0

Query: 43  SSSPSKTISVFSTIPLPPPAADAVDGSGNPASSRKPISLWPGMHHSPVTTALWEARSSIF 102
           +SSPS+TISVFSTI LPP ++DAVDGSG+  SSRKPISLWPGMHHSPVTTALWEARSSIF
Sbjct: 49  NSSPSRTISVFSTITLPP-SSDAVDGSGSSTSSRKPISLWPGMHHSPVTTALWEARSSIF 108

Query: 103 ERLLDPPKDAPPQSELLTKTPSQSRTIVIYKFSSDFILREQYRDPWNEVRIGKLLEDLDA 162
           ERLLDPPKDAPPQSEL TKTPSQSRTIVIY FSSDFILREQYRDPWNEVRIGKLLEDLDA
Sbjct: 109 ERLLDPPKDAPPQSELRTKTPSQSRTIVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDA 168

Query: 163 LAGTISVRHCSDVDSTTRPLLVVTASVDRIVLKKSISVDADLKIVGSVIWVGRSSIEVQL 222
           LAGTISVRHCSD DSTTRPLL+VTASVDRIVLKK ISVDADLKIVGSVIWVGRSSIEVQL
Sbjct: 169 LAGTISVRHCSDDDSTTRPLLLVTASVDRIVLKKPISVDADLKIVGSVIWVGRSSIEVQL 228

Query: 223 DMIQSTEECSNTSDAVALTANFIFVARDSQTGKAALVNRLLPETEEEKLLYEGAEARSKL 282
           DMIQ TE+CSN SD+VALTANFIFVARDSQTGKAALVNRLLPETEEEKLL+EGAEARS L
Sbjct: 229 DMIQGTEDCSNKSDSVALTANFIFVARDSQTGKAALVNRLLPETEEEKLLFEGAEARSNL 288

Query: 283 RKRKNRGDMWELENGDINRLDALLAEGRIFCDMPALADRDSILLRDTGLENSLICQPQQR 342
           RKRKNRGDMW LENG I R+DALLAEGRIFCDMPALADRDSILLRDT LENSLICQPQQR
Sbjct: 289 RKRKNRGDMWGLENGYIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQR 348

Query: 343 NIHGRIFGGFLMQRAFELAFSTAYVFAGMVPYFLEVDHVDFLKPVDVGDFLRFKSCVLYT 402
           NIHGRIFGGFLMQRAFELAFSTAYVFAGMVPYF EVDHVDFLKPVDVGDFLRF SCVLYT
Sbjct: 349 NIHGRIFGGFLMQRAFELAFSTAYVFAGMVPYFYEVDHVDFLKPVDVGDFLRFTSCVLYT 408

Query: 403 EVENPDQPLINVEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKANRNGFRLRNVVPATEE 462
           EV+NPDQPLIN+EVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKAN  GFRLRNVVPATEE
Sbjct: 409 EVDNPDQPLINIEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKANGKGFRLRNVVPATEE 468

Query: 463 EARRIIERMDAEKLLRDLSKQSESGQ 488
           EARRIIERMDAEK+LRDLSKQSESGQ
Sbjct: 469 EARRIIERMDAEKMLRDLSKQSESGQ 493

BLAST of MC09g0804 vs. NCBI nr
Match: XP_022954174.1 (acyl-coenzyme A thioesterase 9, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 785 bits (2028), Expect = 1.36e-283
Identity = 417/478 (87.24%), Postives = 439/478 (91.84%), Query Frame = 0

Query: 12  KVQ-ILRSLNSALPSNVGAAVVVAHKK-SAMDFSSSPSKTISVFSTIPLPPPAADAVDGS 71
           KVQ +++S +S++P +  +     H+  S+MD  SSPS+TISVFSTIPLPP  +DA D S
Sbjct: 7   KVQALIKSWSSSIPKS--SINFQFHRPFSSMDSISSPSRTISVFSTIPLPP-GSDAADAS 66

Query: 72  GNPASSRKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPPQSELLTKTPSQSRTI 131
           GN   SRKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPPQSELLTKTPSQSRTI
Sbjct: 67  GNSTLSRKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPPQSELLTKTPSQSRTI 126

Query: 132 VIYKFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRHCSDVDSTTRPLLVVTASV 191
           VIY FSSDFILREQYRDPWNE RIGKLLEDLDALAGTISVRHCSDVDSTTRPLL+VTASV
Sbjct: 127 VIYNFSSDFILREQYRDPWNEARIGKLLEDLDALAGTISVRHCSDVDSTTRPLLLVTASV 186

Query: 192 DRIVLKKSISVDADLKIVGSVIWVGRSSIEVQLDMIQSTEECSNTSDAVALTANFIFVAR 251
           DRIVLKK ISVDADLK+VGSVIWVG+SSIEVQLDMIQSTE        VALTANFIFVAR
Sbjct: 187 DRIVLKKPISVDADLKVVGSVIWVGKSSIEVQLDMIQSTE--------VALTANFIFVAR 246

Query: 252 DSQTGKAALVNRLLPETEEEKLLYEGAEARSKLRKRKNRGDMWELENGDINRLDALLAEG 311
           DS+TGKAALVNR+LPETEEEKLL+EGAEARSKLRKRKNRG MW LENGDI R+DALLAEG
Sbjct: 247 DSKTGKAALVNRILPETEEEKLLFEGAEARSKLRKRKNRGGMWGLENGDIKRIDALLAEG 306

Query: 312 RIFCDMPALADRDSILLRDTGLENSLICQPQQRNIHGRIFGGFLMQRAFELAFSTAYVFA 371
           RIFCDMPALADRDSILLRDT LENSLICQPQQRNIHGRIFGGFLMQRAFELAFSTAYVFA
Sbjct: 307 RIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGFLMQRAFELAFSTAYVFA 366

Query: 372 GMVPYFLEVDHVDFLKPVDVGDFLRFKSCVLYTEVENPDQPLINVEVVAHVTRPEIRSSE 431
           GMVPYF EVDHVDFLKPVDVGDFLRF SCVLYTE++NPDQPLINVEVVAHVTRPEIRSSE
Sbjct: 367 GMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTELDNPDQPLINVEVVAHVTRPEIRSSE 426

Query: 432 VSNTFYFTFTVRPEAKANRNGFRLRNVVPATEEEARRIIERMDAEKLLRDLSKQSESG 487
           VSNTFYFTFTVRPEAKAN NGFRLRNVVPATEEEARRIIERMDAEK+LRDLSKQSESG
Sbjct: 427 VSNTFYFTFTVRPEAKANGNGFRLRNVVPATEEEARRIIERMDAEKMLRDLSKQSESG 473

BLAST of MC09g0804 vs. NCBI nr
Match: XP_022991674.1 (acyl-coenzyme A thioesterase 9, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 785 bits (2026), Expect = 2.75e-283
Identity = 416/478 (87.03%), Postives = 439/478 (91.84%), Query Frame = 0

Query: 12  KVQ-ILRSLNSALPSNVGAAVVVAHKK-SAMDFSSSPSKTISVFSTIPLPPPAADAVDGS 71
           KVQ +++S NS++P +  +     H+  S+MD  SSPS+TISVFSTIPLPP  +DA D S
Sbjct: 7   KVQALIKSWNSSIPKS--SINFQFHRPFSSMDSISSPSRTISVFSTIPLPP-GSDAADAS 66

Query: 72  GNPASSRKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPPQSELLTKTPSQSRTI 131
           GN   SRKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPPQSELLTKTPSQSRTI
Sbjct: 67  GNSTLSRKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPPQSELLTKTPSQSRTI 126

Query: 132 VIYKFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRHCSDVDSTTRPLLVVTASV 191
           VIY FSSDFILREQYRDPWNE RIGKLLEDLDALAGTISVRHCSDVDSTTRPLL+VTASV
Sbjct: 127 VIYNFSSDFILREQYRDPWNEARIGKLLEDLDALAGTISVRHCSDVDSTTRPLLLVTASV 186

Query: 192 DRIVLKKSISVDADLKIVGSVIWVGRSSIEVQLDMIQSTEECSNTSDAVALTANFIFVAR 251
           DRIVLKK ISVDADLK+VGSVIWVG+SSIEVQLDMIQSTE        VALTANFIFVAR
Sbjct: 187 DRIVLKKPISVDADLKVVGSVIWVGKSSIEVQLDMIQSTE--------VALTANFIFVAR 246

Query: 252 DSQTGKAALVNRLLPETEEEKLLYEGAEARSKLRKRKNRGDMWELENGDINRLDALLAEG 311
           DS+TGKAALVNR+LPETE+EKLL+EGAEARSKLRKRKNRG MW LENGDI R+DALLAEG
Sbjct: 247 DSKTGKAALVNRILPETEKEKLLFEGAEARSKLRKRKNRGGMWGLENGDIKRIDALLAEG 306

Query: 312 RIFCDMPALADRDSILLRDTGLENSLICQPQQRNIHGRIFGGFLMQRAFELAFSTAYVFA 371
           RIFCDMPALADRDSILLRDT LENSLICQPQQRNIHGRIFGGFLMQRAFELAFSTAYVFA
Sbjct: 307 RIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGFLMQRAFELAFSTAYVFA 366

Query: 372 GMVPYFLEVDHVDFLKPVDVGDFLRFKSCVLYTEVENPDQPLINVEVVAHVTRPEIRSSE 431
           GMVPYF EVDHVDFLKPVDVGDFLRF SCVLYTE++NPDQPLINVEVVAHVTRPEIRSSE
Sbjct: 367 GMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTELDNPDQPLINVEVVAHVTRPEIRSSE 426

Query: 432 VSNTFYFTFTVRPEAKANRNGFRLRNVVPATEEEARRIIERMDAEKLLRDLSKQSESG 487
           VSNTFYFTFTVRPEAKAN NGFRLRNVVPATEEEARRIIERMD+EK+LRDLSKQSESG
Sbjct: 427 VSNTFYFTFTVRPEAKANGNGFRLRNVVPATEEEARRIIERMDSEKMLRDLSKQSESG 473

BLAST of MC09g0804 vs. NCBI nr
Match: KAG6576041.1 (Acyl-coenzyme A thioesterase 9, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 779 bits (2011), Expect = 1.39e-280
Identity = 407/447 (91.05%), Postives = 420/447 (93.96%), Query Frame = 0

Query: 40  MDFSSSPSKTISVFSTIPLPPPAADAVDGSGNPASSRKPISLWPGMHHSPVTTALWEARS 99
           MD  SSPS+TISVFSTIPLPP  +DA D SGN   SRKPISLWPGMHHSPVTTALWEARS
Sbjct: 1   MDSISSPSRTISVFSTIPLPP-GSDAADASGNSTLSRKPISLWPGMHHSPVTTALWEARS 60

Query: 100 SIFERLLDPPKDAPPQSELLTKTPSQSRTIVIYKFSSDFILREQYRDPWNEVRIGKLLED 159
           SIFERLLDPPKDAPPQSELLTKTPSQSRTIVIY FSSDFILREQYRDPWNE RIGKLLED
Sbjct: 61  SIFERLLDPPKDAPPQSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNEARIGKLLED 120

Query: 160 LDALAGTISVRHCSDVDSTTRPLLVVTASVDRIVLKKSISVDADLKIVGSVIWVGRSSIE 219
           LDALAGTISVRHCSDVDSTTRPLL+VTASVDRIVLKK ISVDADLK+VGSVIWVG+SSIE
Sbjct: 121 LDALAGTISVRHCSDVDSTTRPLLLVTASVDRIVLKKPISVDADLKVVGSVIWVGKSSIE 180

Query: 220 VQLDMIQSTEECSNTSDAVALTANFIFVARDSQTGKAALVNRLLPETEEEKLLYEGAEAR 279
           VQLDMIQSTE        VALTANFIFVARDS+TGKAALVNR+LPET+EEKLL+EGAEAR
Sbjct: 181 VQLDMIQSTE--------VALTANFIFVARDSKTGKAALVNRILPETDEEKLLFEGAEAR 240

Query: 280 SKLRKRKNRGDMWELENGDINRLDALLAEGRIFCDMPALADRDSILLRDTGLENSLICQP 339
           SKLRKRKNRG MW LENGDI R+DALLAEGRIFCDMPALADRDSILLRDT LENSLICQP
Sbjct: 241 SKLRKRKNRGGMWGLENGDIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQP 300

Query: 340 QQRNIHGRIFGGFLMQRAFELAFSTAYVFAGMVPYFLEVDHVDFLKPVDVGDFLRFKSCV 399
           QQRNIHGRIFGGFLMQRAFELAFSTAYVFAGMVPYF EVDHVDFLKPVDVGDFLRF SCV
Sbjct: 301 QQRNIHGRIFGGFLMQRAFELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCV 360

Query: 400 LYTEVENPDQPLINVEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKANRNGFRLRNVVPA 459
           LYTE++NPDQPLINVEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKAN NGFRLRNVVPA
Sbjct: 361 LYTELDNPDQPLINVEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKANGNGFRLRNVVPA 420

Query: 460 TEEEARRIIERMDAEKLLRDLSKQSES 486
           TEEEARRIIERMDAEK+LRDLSKQSES
Sbjct: 421 TEEEARRIIERMDAEKMLRDLSKQSES 438

BLAST of MC09g0804 vs. ExPASy TrEMBL
Match: A0A6J1CP70 (acyl-coenzyme A thioesterase 9, mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC111013446 PE=4 SV=1)

HSP 1 Score: 945 bits (2443), Expect = 0.0
Identity = 489/489 (100.00%), Postives = 489/489 (100.00%), Query Frame = 0

Query: 1   MGPGSLFLWGRKVQILRSLNSALPSNVGAAVVVAHKKSAMDFSSSPSKTISVFSTIPLPP 60
           MGPGSLFLWGRKVQILRSLNSALPSNVGAAVVVAHKKSAMDFSSSPSKTISVFSTIPLPP
Sbjct: 1   MGPGSLFLWGRKVQILRSLNSALPSNVGAAVVVAHKKSAMDFSSSPSKTISVFSTIPLPP 60

Query: 61  PAADAVDGSGNPASSRKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPPQSELLT 120
           PAADAVDGSGNPASSRKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPPQSELLT
Sbjct: 61  PAADAVDGSGNPASSRKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPPQSELLT 120

Query: 121 KTPSQSRTIVIYKFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRHCSDVDSTTR 180
           KTPSQSRTIVIYKFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRHCSDVDSTTR
Sbjct: 121 KTPSQSRTIVIYKFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRHCSDVDSTTR 180

Query: 181 PLLVVTASVDRIVLKKSISVDADLKIVGSVIWVGRSSIEVQLDMIQSTEECSNTSDAVAL 240
           PLLVVTASVDRIVLKKSISVDADLKIVGSVIWVGRSSIEVQLDMIQSTEECSNTSDAVAL
Sbjct: 181 PLLVVTASVDRIVLKKSISVDADLKIVGSVIWVGRSSIEVQLDMIQSTEECSNTSDAVAL 240

Query: 241 TANFIFVARDSQTGKAALVNRLLPETEEEKLLYEGAEARSKLRKRKNRGDMWELENGDIN 300
           TANFIFVARDSQTGKAALVNRLLPETEEEKLLYEGAEARSKLRKRKNRGDMWELENGDIN
Sbjct: 241 TANFIFVARDSQTGKAALVNRLLPETEEEKLLYEGAEARSKLRKRKNRGDMWELENGDIN 300

Query: 301 RLDALLAEGRIFCDMPALADRDSILLRDTGLENSLICQPQQRNIHGRIFGGFLMQRAFEL 360
           RLDALLAEGRIFCDMPALADRDSILLRDTGLENSLICQPQQRNIHGRIFGGFLMQRAFEL
Sbjct: 301 RLDALLAEGRIFCDMPALADRDSILLRDTGLENSLICQPQQRNIHGRIFGGFLMQRAFEL 360

Query: 361 AFSTAYVFAGMVPYFLEVDHVDFLKPVDVGDFLRFKSCVLYTEVENPDQPLINVEVVAHV 420
           AFSTAYVFAGMVPYFLEVDHVDFLKPVDVGDFLRFKSCVLYTEVENPDQPLINVEVVAHV
Sbjct: 361 AFSTAYVFAGMVPYFLEVDHVDFLKPVDVGDFLRFKSCVLYTEVENPDQPLINVEVVAHV 420

Query: 421 TRPEIRSSEVSNTFYFTFTVRPEAKANRNGFRLRNVVPATEEEARRIIERMDAEKLLRDL 480
           TRPEIRSSEVSNTFYFTFTVRPEAKANRNGFRLRNVVPATEEEARRIIERMDAEKLLRDL
Sbjct: 421 TRPEIRSSEVSNTFYFTFTVRPEAKANRNGFRLRNVVPATEEEARRIIERMDAEKLLRDL 480

Query: 481 SKQSESGQM 489
           SKQSESGQM
Sbjct: 481 SKQSESGQM 489

BLAST of MC09g0804 vs. ExPASy TrEMBL
Match: A0A6J1GS66 (acyl-coenzyme A thioesterase 9, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111456516 PE=4 SV=1)

HSP 1 Score: 785 bits (2028), Expect = 6.61e-284
Identity = 417/478 (87.24%), Postives = 439/478 (91.84%), Query Frame = 0

Query: 12  KVQ-ILRSLNSALPSNVGAAVVVAHKK-SAMDFSSSPSKTISVFSTIPLPPPAADAVDGS 71
           KVQ +++S +S++P +  +     H+  S+MD  SSPS+TISVFSTIPLPP  +DA D S
Sbjct: 7   KVQALIKSWSSSIPKS--SINFQFHRPFSSMDSISSPSRTISVFSTIPLPP-GSDAADAS 66

Query: 72  GNPASSRKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPPQSELLTKTPSQSRTI 131
           GN   SRKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPPQSELLTKTPSQSRTI
Sbjct: 67  GNSTLSRKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPPQSELLTKTPSQSRTI 126

Query: 132 VIYKFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRHCSDVDSTTRPLLVVTASV 191
           VIY FSSDFILREQYRDPWNE RIGKLLEDLDALAGTISVRHCSDVDSTTRPLL+VTASV
Sbjct: 127 VIYNFSSDFILREQYRDPWNEARIGKLLEDLDALAGTISVRHCSDVDSTTRPLLLVTASV 186

Query: 192 DRIVLKKSISVDADLKIVGSVIWVGRSSIEVQLDMIQSTEECSNTSDAVALTANFIFVAR 251
           DRIVLKK ISVDADLK+VGSVIWVG+SSIEVQLDMIQSTE        VALTANFIFVAR
Sbjct: 187 DRIVLKKPISVDADLKVVGSVIWVGKSSIEVQLDMIQSTE--------VALTANFIFVAR 246

Query: 252 DSQTGKAALVNRLLPETEEEKLLYEGAEARSKLRKRKNRGDMWELENGDINRLDALLAEG 311
           DS+TGKAALVNR+LPETEEEKLL+EGAEARSKLRKRKNRG MW LENGDI R+DALLAEG
Sbjct: 247 DSKTGKAALVNRILPETEEEKLLFEGAEARSKLRKRKNRGGMWGLENGDIKRIDALLAEG 306

Query: 312 RIFCDMPALADRDSILLRDTGLENSLICQPQQRNIHGRIFGGFLMQRAFELAFSTAYVFA 371
           RIFCDMPALADRDSILLRDT LENSLICQPQQRNIHGRIFGGFLMQRAFELAFSTAYVFA
Sbjct: 307 RIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGFLMQRAFELAFSTAYVFA 366

Query: 372 GMVPYFLEVDHVDFLKPVDVGDFLRFKSCVLYTEVENPDQPLINVEVVAHVTRPEIRSSE 431
           GMVPYF EVDHVDFLKPVDVGDFLRF SCVLYTE++NPDQPLINVEVVAHVTRPEIRSSE
Sbjct: 367 GMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTELDNPDQPLINVEVVAHVTRPEIRSSE 426

Query: 432 VSNTFYFTFTVRPEAKANRNGFRLRNVVPATEEEARRIIERMDAEKLLRDLSKQSESG 487
           VSNTFYFTFTVRPEAKAN NGFRLRNVVPATEEEARRIIERMDAEK+LRDLSKQSESG
Sbjct: 427 VSNTFYFTFTVRPEAKANGNGFRLRNVVPATEEEARRIIERMDAEKMLRDLSKQSESG 473

BLAST of MC09g0804 vs. ExPASy TrEMBL
Match: A0A6J1JVH9 (acyl-coenzyme A thioesterase 9, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111488217 PE=4 SV=1)

HSP 1 Score: 785 bits (2026), Expect = 1.33e-283
Identity = 416/478 (87.03%), Postives = 439/478 (91.84%), Query Frame = 0

Query: 12  KVQ-ILRSLNSALPSNVGAAVVVAHKK-SAMDFSSSPSKTISVFSTIPLPPPAADAVDGS 71
           KVQ +++S NS++P +  +     H+  S+MD  SSPS+TISVFSTIPLPP  +DA D S
Sbjct: 7   KVQALIKSWNSSIPKS--SINFQFHRPFSSMDSISSPSRTISVFSTIPLPP-GSDAADAS 66

Query: 72  GNPASSRKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPPQSELLTKTPSQSRTI 131
           GN   SRKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPPQSELLTKTPSQSRTI
Sbjct: 67  GNSTLSRKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPPQSELLTKTPSQSRTI 126

Query: 132 VIYKFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRHCSDVDSTTRPLLVVTASV 191
           VIY FSSDFILREQYRDPWNE RIGKLLEDLDALAGTISVRHCSDVDSTTRPLL+VTASV
Sbjct: 127 VIYNFSSDFILREQYRDPWNEARIGKLLEDLDALAGTISVRHCSDVDSTTRPLLLVTASV 186

Query: 192 DRIVLKKSISVDADLKIVGSVIWVGRSSIEVQLDMIQSTEECSNTSDAVALTANFIFVAR 251
           DRIVLKK ISVDADLK+VGSVIWVG+SSIEVQLDMIQSTE        VALTANFIFVAR
Sbjct: 187 DRIVLKKPISVDADLKVVGSVIWVGKSSIEVQLDMIQSTE--------VALTANFIFVAR 246

Query: 252 DSQTGKAALVNRLLPETEEEKLLYEGAEARSKLRKRKNRGDMWELENGDINRLDALLAEG 311
           DS+TGKAALVNR+LPETE+EKLL+EGAEARSKLRKRKNRG MW LENGDI R+DALLAEG
Sbjct: 247 DSKTGKAALVNRILPETEKEKLLFEGAEARSKLRKRKNRGGMWGLENGDIKRIDALLAEG 306

Query: 312 RIFCDMPALADRDSILLRDTGLENSLICQPQQRNIHGRIFGGFLMQRAFELAFSTAYVFA 371
           RIFCDMPALADRDSILLRDT LENSLICQPQQRNIHGRIFGGFLMQRAFELAFSTAYVFA
Sbjct: 307 RIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGFLMQRAFELAFSTAYVFA 366

Query: 372 GMVPYFLEVDHVDFLKPVDVGDFLRFKSCVLYTEVENPDQPLINVEVVAHVTRPEIRSSE 431
           GMVPYF EVDHVDFLKPVDVGDFLRF SCVLYTE++NPDQPLINVEVVAHVTRPEIRSSE
Sbjct: 367 GMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTELDNPDQPLINVEVVAHVTRPEIRSSE 426

Query: 432 VSNTFYFTFTVRPEAKANRNGFRLRNVVPATEEEARRIIERMDAEKLLRDLSKQSESG 487
           VSNTFYFTFTVRPEAKAN NGFRLRNVVPATEEEARRIIERMD+EK+LRDLSKQSESG
Sbjct: 427 VSNTFYFTFTVRPEAKANGNGFRLRNVVPATEEEARRIIERMDSEKMLRDLSKQSESG 473

BLAST of MC09g0804 vs. ExPASy TrEMBL
Match: A0A0A0KA74 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G373540 PE=4 SV=1)

HSP 1 Score: 774 bits (1998), Expect = 4.43e-279
Identity = 401/442 (90.72%), Postives = 419/442 (94.80%), Query Frame = 0

Query: 34  AHKKSAMDFSSSPSKTISVFSTIPLPPPAADAVDGSGNPASSRKPISLWPGMHHSPVTTA 93
           A+  S  + +SSPS+TISVFSTIPLPP ++DAV+GSG+  SSRKPI+LWPGMHHSPVT A
Sbjct: 40  ANSSSNPNSNSSPSRTISVFSTIPLPP-SSDAVNGSGSSTSSRKPINLWPGMHHSPVTAA 99

Query: 94  LWEARSSIFERLLDPPKDAPPQSELLTKTPSQSRTIVIYKFSSDFILREQYRDPWNEVRI 153
           LW+ARSSIFERLLDPPKDAPPQSELLTKTPSQSRTIVIY FSSDFILREQYRDPWNEVRI
Sbjct: 100 LWDARSSIFERLLDPPKDAPPQSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNEVRI 159

Query: 154 GKLLEDLDALAGTISVRHCSDVDSTTRPLLVVTASVDRIVLKKSISVDADLKIVGSVIWV 213
           GKLLEDLDALAGTISVRHCSD DSTTRPLL+VTASVDRIVLKK ISVDADLKIVGSVIWV
Sbjct: 160 GKLLEDLDALAGTISVRHCSDDDSTTRPLLLVTASVDRIVLKKPISVDADLKIVGSVIWV 219

Query: 214 GRSSIEVQLDMIQSTEECSNTSDAVALTANFIFVARDSQTGKAALVNRLLPETEEEKLLY 273
           GRSSIEVQLDMIQ T++CSN SD+VALTANFIFVARDSQTGKAALVNRLLPETEEEKLL+
Sbjct: 220 GRSSIEVQLDMIQGTKDCSNKSDSVALTANFIFVARDSQTGKAALVNRLLPETEEEKLLF 279

Query: 274 EGAEARSKLRKRKNRGDMWELENGDINRLDALLAEGRIFCDMPALADRDSILLRDTGLEN 333
           EGAEARS LRKRKNRGDMW LENG I R+DALLAEGRIFCDMPALADRDSILLRDT LEN
Sbjct: 280 EGAEARSNLRKRKNRGDMWALENGYIKRIDALLAEGRIFCDMPALADRDSILLRDTRLEN 339

Query: 334 SLICQPQQRNIHGRIFGGFLMQRAFELAFSTAYVFAGMVPYFLEVDHVDFLKPVDVGDFL 393
           SLICQPQQRNIHGRIFGGFLMQRAFELAFSTAYVFAGMVPYF EVDHVDFLKPVDVGDFL
Sbjct: 340 SLICQPQQRNIHGRIFGGFLMQRAFELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFL 399

Query: 394 RFKSCVLYTEVENPDQPLINVEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKANRNGFRL 453
           RF SCVLYTEV+NPDQPLINVEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKAN  GFRL
Sbjct: 400 RFTSCVLYTEVDNPDQPLINVEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKANGKGFRL 459

Query: 454 RNVVPATEEEARRIIERMDAEK 475
           R+VVPATEEEARRIIERMDAEK
Sbjct: 460 RSVVPATEEEARRIIERMDAEK 480

BLAST of MC09g0804 vs. ExPASy TrEMBL
Match: A0A1S3BGC8 (acyl-coenzyme A thioesterase 9, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103489713 PE=4 SV=1)

HSP 1 Score: 770 bits (1989), Expect = 1.04e-277
Identity = 398/442 (90.05%), Postives = 417/442 (94.34%), Query Frame = 0

Query: 34  AHKKSAMDFSSSPSKTISVFSTIPLPPPAADAVDGSGNPASSRKPISLWPGMHHSPVTTA 93
           A+  S  +F+SSPS+TISVFSTIPLPP ++DAV+GSG+  SSRKPI+LWPGMHHSPVT A
Sbjct: 40  ANSNSNSNFNSSPSRTISVFSTIPLPP-SSDAVNGSGSSTSSRKPINLWPGMHHSPVTAA 99

Query: 94  LWEARSSIFERLLDPPKDAPPQSELLTKTPSQSRTIVIYKFSSDFILREQYRDPWNEVRI 153
           LW+ARSSIFERLLDPPKDAPPQSELLTKTPSQSR IVIY FSSDFILREQYRDPWNEVRI
Sbjct: 100 LWDARSSIFERLLDPPKDAPPQSELLTKTPSQSRIIVIYNFSSDFILREQYRDPWNEVRI 159

Query: 154 GKLLEDLDALAGTISVRHCSDVDSTTRPLLVVTASVDRIVLKKSISVDADLKIVGSVIWV 213
           GKLLEDLDALAGTISVRHCSD DSTTRPLL+VTASVDRIVLKK ISV ADLKIVGSVIWV
Sbjct: 160 GKLLEDLDALAGTISVRHCSDDDSTTRPLLLVTASVDRIVLKKPISVVADLKIVGSVIWV 219

Query: 214 GRSSIEVQLDMIQSTEECSNTSDAVALTANFIFVARDSQTGKAALVNRLLPETEEEKLLY 273
           GRSSIEVQLDMIQ TE+CSN SD+VALTANFIFVARDSQTGKAALVNRLLPETEEEKLL+
Sbjct: 220 GRSSIEVQLDMIQGTEDCSNKSDSVALTANFIFVARDSQTGKAALVNRLLPETEEEKLLF 279

Query: 274 EGAEARSKLRKRKNRGDMWELENGDINRLDALLAEGRIFCDMPALADRDSILLRDTGLEN 333
           EGAEARS LRKRKNRGDMW LENG + R+DALLAEGRIFCDMPALADRDSILLRDT LEN
Sbjct: 280 EGAEARSNLRKRKNRGDMWGLENGYLKRIDALLAEGRIFCDMPALADRDSILLRDTRLEN 339

Query: 334 SLICQPQQRNIHGRIFGGFLMQRAFELAFSTAYVFAGMVPYFLEVDHVDFLKPVDVGDFL 393
           SLICQPQQRNIHGRIFGGFLMQRAFELAFSTAYVFAGMVPYF EVDHVDFLKPVDVGDFL
Sbjct: 340 SLICQPQQRNIHGRIFGGFLMQRAFELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFL 399

Query: 394 RFKSCVLYTEVENPDQPLINVEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKANRNGFRL 453
           RF SCVLYTEV+NPD PLIN+EVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKAN  GFRL
Sbjct: 400 RFTSCVLYTEVDNPDHPLINIEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKANGKGFRL 459

Query: 454 RNVVPATEEEARRIIERMDAEK 475
           RNVVPATEEEARRIIERMDAE+
Sbjct: 460 RNVVPATEEEARRIIERMDAER 480

BLAST of MC09g0804 vs. TAIR 10
Match: AT5G48370.2 (Thioesterase/thiol ester dehydrase-isomerase superfamily protein )

HSP 1 Score: 638.3 bits (1645), Expect = 5.2e-183
Identity = 328/429 (76.46%), Postives = 366/429 (85.31%), Query Frame = 0

Query: 48  KTISVFSTIPLPPPAADAVDGSGNPASSRKPISLWPGMHHSPVTTALWEARSSIFERLLD 107
           + ISV ST   P   +D          +RKP+SLWPGM+HSPVTTALWEARS IFE LLD
Sbjct: 5   RPISVVSTFASPSSTSD---------PTRKPLSLWPGMYHSPVTTALWEARSKIFESLLD 64

Query: 108 PPKDAPPQSELLTKTPSQSRTIVIYKFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTI 167
           PPKDAPPQS+LLT+TPS SRT + Y FS+DFILREQYRDPWNEVRIG LLEDLDALAGTI
Sbjct: 65  PPKDAPPQSQLLTRTPSHSRTTIFYPFSTDFILREQYRDPWNEVRIGILLEDLDALAGTI 124

Query: 168 SVRHCSDVDSTTRPLLVVTASVDRIVLKKSISVDADLKIVGSVIWVGRSSIEVQLDMIQS 227
           SV+HCSD DSTTRPLL+VTASV +IVLKK I VD DLKIV SVIWVGRSSIE+QL+++QS
Sbjct: 125 SVKHCSDDDSTTRPLLLVTASVHKIVLKKPICVDIDLKIVASVIWVGRSSIEIQLEVMQS 184

Query: 228 TEECSNTSDAVALTANFIFVARDSQTGKAALVNRLLPETEEEKLLYEGAEARSKLRKRKN 287
                 +SD+VALTANFIFVARDS+TGKAA +NRL PETE EKLL+E AEAR+ LRK+K 
Sbjct: 185 ELNVKASSDSVALTANFIFVARDSKTGKAAPINRLSPETEVEKLLFEEAEARNNLRKKKR 244

Query: 288 RGDMWELENGDINRLDALLAEGRIFCDMPALADRDSILLRDTGLENSLICQPQQRNIHGR 347
            GD  E ++G+  +L+A LAEGRIF DMPALADR+SILL+DT LENSLICQPQQRNIHGR
Sbjct: 245 GGDRREFDHGECKKLEAWLAEGRIFSDMPALADRNSILLKDTRLENSLICQPQQRNIHGR 304

Query: 348 IFGGFLMQRAFELAFSTAYVFAGMVPYFLEVDHVDFLKPVDVGDFLRFKSCVLYTEVENP 407
           IFGGFLM RAFELAFSTAY FAG+VPYFLEVDHVDFL+PVDVGDFLRFKSCVLYT+++  
Sbjct: 305 IFGGFLMHRAFELAFSTAYTFAGLVPYFLEVDHVDFLRPVDVGDFLRFKSCVLYTQLDKQ 364

Query: 408 DQPLINVEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKANRNGFRLRNVVPATEEEARRI 467
           D PLIN+EVVAHVT PEIRSSEVSNTFYF FTVRPEAKA  NGF+LRNVVPATEEEAR I
Sbjct: 365 DCPLINIEVVAHVTSPEIRSSEVSNTFYFKFTVRPEAKARNNGFKLRNVVPATEEEARHI 424

Query: 468 IERMDAEKL 477
           +ERMDAE L
Sbjct: 425 LERMDAEAL 424

BLAST of MC09g0804 vs. TAIR 10
Match: AT5G48370.1 (Thioesterase/thiol ester dehydrase-isomerase superfamily protein )

HSP 1 Score: 636.0 bits (1639), Expect = 2.6e-182
Identity = 328/430 (76.28%), Postives = 368/430 (85.58%), Query Frame = 0

Query: 48  KTISVFSTIPLPPPAADAVDGSGNPASSRKPISLWPGMHHSPVTTALWEARSSIFERLLD 107
           + ISV ST   P   +D          +RKP+SLWPGM+HSPVTTALWEARS IFE LLD
Sbjct: 5   RPISVVSTFASPSSTSD---------PTRKPLSLWPGMYHSPVTTALWEARSKIFESLLD 64

Query: 108 PPKDAPPQSELLTKTPSQSRTIVIYKFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTI 167
           PPKDAPPQS+LLT+TPS SRT + Y FS+DFILREQYRDPWNEVRIG LLEDLDALAGTI
Sbjct: 65  PPKDAPPQSQLLTRTPSHSRTTIFYPFSTDFILREQYRDPWNEVRIGILLEDLDALAGTI 124

Query: 168 SVRHCSDVDSTTRPLLVVTASVDRIVLKKSISVDADLKIVGSVIWVGRSSIEVQLDMIQS 227
           SV+HCSD DSTTRPLL+VTASV +IVLKK I VD DLKIV SVIWVGRSSIE+QL+++QS
Sbjct: 125 SVKHCSDDDSTTRPLLLVTASVHKIVLKKPICVDIDLKIVASVIWVGRSSIEIQLEVMQS 184

Query: 228 -TEECSNTSDAVALTANFIFVARDSQTGKAALVNRLLPETEEEKLLYEGAEARSKLRKRK 287
             ++   +SD+VALTANFIFVARDS+TGKAA +NRL PETE EKLL+E AEAR+ LRK+K
Sbjct: 185 ELKDVKASSDSVALTANFIFVARDSKTGKAAPINRLSPETEVEKLLFEEAEARNNLRKKK 244

Query: 288 NRGDMWELENGDINRLDALLAEGRIFCDMPALADRDSILLRDTGLENSLICQPQQRNIHG 347
             GD  E ++G+  +L+A LAEGRIF DMPALADR+SILL+DT LENSLICQPQQRNIHG
Sbjct: 245 RGGDRREFDHGECKKLEAWLAEGRIFSDMPALADRNSILLKDTRLENSLICQPQQRNIHG 304

Query: 348 RIFGGFLMQRAFELAFSTAYVFAGMVPYFLEVDHVDFLKPVDVGDFLRFKSCVLYTEVEN 407
           RIFGGFLM RAFELAFSTAY FAG+VPYFLEVDHVDFL+PVDVGDFLRFKSCVLYT+++ 
Sbjct: 305 RIFGGFLMHRAFELAFSTAYTFAGLVPYFLEVDHVDFLRPVDVGDFLRFKSCVLYTQLDK 364

Query: 408 PDQPLINVEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKANRNGFRLRNVVPATEEEARR 467
            D PLIN+EVVAHVT PEIRSSEVSNTFYF FTVRPEAKA  NGF+LRNVVPATEEEAR 
Sbjct: 365 QDCPLINIEVVAHVTSPEIRSSEVSNTFYFKFTVRPEAKARNNGFKLRNVVPATEEEARH 424

Query: 468 IIERMDAEKL 477
           I+ERMDAE L
Sbjct: 425 ILERMDAEAL 425

BLAST of MC09g0804 vs. TAIR 10
Match: AT2G30720.1 (Thioesterase/thiol ester dehydrase-isomerase superfamily protein )

HSP 1 Score: 526.9 bits (1356), Expect = 1.7e-149
Identity = 276/424 (65.09%), Postives = 344/424 (81.13%), Query Frame = 0

Query: 54  STIPLPP-PAADAVDGSGNPASSRKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDA 113
           ST+P    P  +++D     +S RKPISLWPGM+HSPVT ALWEAR ++FE    P  D 
Sbjct: 37  STVPSDENPDQNSIDAG---SSMRKPISLWPGMYHSPVTNALWEARRNMFE---IPTGDD 96

Query: 114 PPQSELLTKTPSQSRTIVIYKFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRHC 173
             QS+L  K+PS+SRT ++YKFSSDF+LREQYR+PWNE+R GKL+EDLDALAGTIS +HC
Sbjct: 97  ASQSKLTAKSPSRSRTSILYKFSSDFVLREQYRNPWNEIRTGKLVEDLDALAGTISFKHC 156

Query: 174 SDVDSTTRPLLVVTASVDRIVLKKSISVDADLKIVGSVIWVGRSSIEVQLDMIQSTEECS 233
              DS+ R +++VTASVDRI++K+ I VD DL IVG+V WVGRSS+E+QL ++Q  ++ +
Sbjct: 157 GG-DSSARSMILVTASVDRIIMKRPIRVDTDLSIVGAVTWVGRSSMEMQLQVLQ-IQDTN 216

Query: 234 NTSDAVALTANFIFVARDSQTGKAALVNRLLPETEEEKLLYEGAEARSKLRKRKNRGDMW 293
           N+S++VAL ANF FVARD+QTGK+A +N+++PETE EK L++ AE R+KLRK+K      
Sbjct: 217 NSSESVALEANFTFVARDAQTGKSAPINQVVPETEHEKFLWKEAEERNKLRKQKRAQGKE 276

Query: 294 ELEN-GDINRLDALLAEGRIFCDMPALADRDSILLRDTGLENSLICQPQQRNIHGRIFGG 353
           E E   D+ RL+ LLAEGR+F DMPALADR+SIL++DT  ENSLICQPQQRNIHGRIFGG
Sbjct: 277 EHEKLKDLERLNELLAEGRVFLDMPALADRNSILIKDTSHENSLICQPQQRNIHGRIFGG 336

Query: 354 FLMQRAFELAFSTAYVFAGMVPYFLEVDHVDFLKPVDVGDFLRFKSCVLYTEVENPDQPL 413
           FLM++AFELAFS AY FAG+ P FLEVD VDF+KPVDVG+FLRFKS VLYTE  +  +PL
Sbjct: 337 FLMRKAFELAFSNAYTFAGVSPRFLEVDRVDFIKPVDVGNFLRFKSRVLYTEATSSAEPL 396

Query: 414 INVEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKANRNGFRLRNVVPATEEEARRIIERM 473
           IN+EVVAHVT PE+RSSEVSN FYFTF+VRPEA   ++G ++RNVVPATEEEARR+IERM
Sbjct: 397 INIEVVAHVTSPELRSSEVSNRFYFTFSVRPEAM--KDGLKIRNVVPATEEEARRVIERM 450

Query: 474 DAEK 476
           DAE+
Sbjct: 457 DAER 450

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
S4TDL23.7e-19479.86Acyl-coenzyme A thioesterase 2, chloroplastic OS=Humulus lupulus OX=3486 GN=TE2 ... [more]
S4TF941.9e-15866.29Acyl-coenzyme A thioesterase 4, mitochondrial OS=Humulus lupulus OX=3486 GN=TE4 ... [more]
Q9R0X42.1e-4034.38Acyl-coenzyme A thioesterase 9, mitochondrial OS=Mus musculus OX=10090 GN=Acot9 ... [more]
Q9Y3054.8e-4034.12Acyl-coenzyme A thioesterase 9, mitochondrial OS=Homo sapiens OX=9606 GN=ACOT9 P... [more]
Q32MW31.5e-3833.44Acyl-coenzyme A thioesterase 10, mitochondrial OS=Mus musculus OX=10090 GN=Acot1... [more]
Match NameE-valueIdentityDescription
XP_022143585.10.0100.00acyl-coenzyme A thioesterase 9, mitochondrial-like [Momordica charantia][more]
XP_038899833.18.09e-28892.83LOW QUALITY PROTEIN: acyl-coenzyme A thioesterase 9, mitochondrial-like [Beninca... [more]
XP_022954174.11.36e-28387.24acyl-coenzyme A thioesterase 9, mitochondrial-like [Cucurbita moschata][more]
XP_022991674.12.75e-28387.03acyl-coenzyme A thioesterase 9, mitochondrial-like [Cucurbita maxima][more]
KAG6576041.11.39e-28091.05Acyl-coenzyme A thioesterase 9, mitochondrial, partial [Cucurbita argyrosperma s... [more]
Match NameE-valueIdentityDescription
A0A6J1CP700.0100.00acyl-coenzyme A thioesterase 9, mitochondrial-like OS=Momordica charantia OX=367... [more]
A0A6J1GS666.61e-28487.24acyl-coenzyme A thioesterase 9, mitochondrial-like OS=Cucurbita moschata OX=3662... [more]
A0A6J1JVH91.33e-28387.03acyl-coenzyme A thioesterase 9, mitochondrial-like OS=Cucurbita maxima OX=3661 G... [more]
A0A0A0KA744.43e-27990.72Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G373540 PE=4 SV=1[more]
A0A1S3BGC81.04e-27790.05acyl-coenzyme A thioesterase 9, mitochondrial OS=Cucumis melo OX=3656 GN=LOC1034... [more]
Match NameE-valueIdentityDescription
AT5G48370.25.2e-18376.46Thioesterase/thiol ester dehydrase-isomerase superfamily protein [more]
AT5G48370.12.6e-18276.28Thioesterase/thiol ester dehydrase-isomerase superfamily protein [more]
AT2G30720.11.7e-14965.09Thioesterase/thiol ester dehydrase-isomerase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.10.129.10Hotdog Thioesterasecoord: 125..295
e-value: 1.9E-42
score: 147.0
NoneNo IPR availableGENE3D3.10.129.10Hotdog Thioesterasecoord: 319..486
e-value: 2.2E-39
score: 136.9
NoneNo IPR availablePANTHERPTHR12655:SF3BNAA04G17790D PROTEINcoord: 43..475
NoneNo IPR availablePANTHERPTHR12655ACYL-COA THIOESTERASEcoord: 43..475
NoneNo IPR availableCDDcd03442BFIT_BACHcoord: 328..443
e-value: 3.26433E-30
score: 112.277
NoneNo IPR availableCDDcd03442BFIT_BACHcoord: 127..260
e-value: 2.85105E-28
score: 106.884
IPR033120Hotdog acyl-CoA thioesterase (ACOT)-type domainPROSITEPS51770HOTDOG_ACOTcoord: 327..444
score: 28.2862
IPR033120Hotdog acyl-CoA thioesterase (ACOT)-type domainPROSITEPS51770HOTDOG_ACOTcoord: 130..252
score: 21.626856
IPR029069HotDog domain superfamilySUPERFAMILY54637Thioesterase/thiol ester dehydrase-isomerasecoord: 134..286
IPR029069HotDog domain superfamilySUPERFAMILY54637Thioesterase/thiol ester dehydrase-isomerasecoord: 326..476

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g0804.1MC09g0804.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006637 acyl-CoA metabolic process
molecular_function GO:0047617 acyl-CoA hydrolase activity