MC09g0727 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g0727
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptiontRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit
LocationMC09: 6813619 .. 6818809 (-)
RNA-Seq ExpressionMC09g0727
SyntenyMC09g0727
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCTAAAACTAAAATTAAATTCCATGGTGTCATCTTTAGAAAACAAAACATTATAAAAAAAAATATACAAAGGGTATTTTGGTAAAAGTAACGTTTGAAATGGAACATTCCCCCCAAATTTTTCTCACGAAAACCCTAATTTTGCAGTTCAAGAAAACGCCCGTATTTCAGTTCACCGGAAGAATGGAGGAAACGCCGGTAGAGGAAGGAGATACCAACAAAGATGTCGAGGTTGCTCCGGCCTTAATTGCGGTTCACCCGGCCGAGAAATCTGTTGCCGTCGCCGTTGGGTCCGACCTGCGCGTATTCGATCTCGTGTGAGTTTTCTCGGATCGATTAACTCGATTCTTCTGTTTATTTTCCTTTTGAAACTCAATCTATTCCTATAGAATTGCGTTGGCGCTTGCAATACGATTGTAGTCACTATCTTCGTACTAATTTTGTTTTAGCTCTGATGGGTTGTTCTGAGTTAACTGCAGAGGTAATTGTCCGGTTTCTTTGGTCGATGAATCTGGGGAACAGCATCATAAAGATTCCATTAGAGCTATTTGTTATGGTTCAAAAGGGCAGCTTTTTGTATCCGCTGGTGATGACAAAACTGTTAAGATTTGGTCGACTGAGTCTTGGCGCTGCATTTATAACGTGTAAGTTTTTTCCCCCTCTCCTGTCTCTCTCCTTTTTTTTTTCCTGGGTGATTTATAGCGAGGGGATTGATTGGCTGTAGATTTGTAGCGAGGGGATTGATTGGCTGTAGATTTTTAGTTTGATTGGTGAACTCTTTCAGCATTTCCTCGTGCATATTTGCTCAATGAATTGGCACACCATCTAACGGCATAATCTTGCACAAATTTCAATTTTTTTGGGTTTTTAAAGTGGGACTGTACTCAGCATCTAAATTGCAAGCGTATAGTAGCTTCTGGATTATCTGGAAAATTGTAACACCAGCTGCTTGCTTTATCGATTGGTCTTACTTGTGAAATACTGAAGTTTTCATGATTCTCCTGACAGGTGTTCTGAAAAACGAGTCAGTGCAGCTGCCATTAGTCCCGACGGGTTACATGTTTGTTTTGCTGACAAGTTTGGGGTGATTTGGGTTGTGGATCTTGATGGAGGGATTGATGAAAATCAACCTCTTGCCAACAAGACGGCTGTGTCGTTGCTTTCACATTATTGCAGTATAATCACTAGCTTGGTAAGCAGTTCTCTGTTAGCCATCCTGTTTCAGCATTTAGCAATGTTTGGTGTCTTGAGTGTATTACTAGATTTTTCTTAATTTTTTCTTCTTATTATTTTTAACTTTCACTATGGTGCCCGTGTGATTGTTTTTTATTTGCTTTATTTCTGGAACTCATTTCGTTAGCTTGTACAATGTCTTTTTGGCGTCAAATACTATGATTTTTATTAATAATATTAATAAAAACGTCAATAAGGGTTTTCATACTCATGTATATTGTAGAAATTAATTTTTAATTTTTATGTTATTTTTTGTCGCTGCCTCTCATTTCTATGTTATCAACTTCCTATTTGAGGAGAGACTCAATATTTCTTTATTTGAGAGATGAATTGTATAGAGTGCAGCAAAGATGGGTCATAGTTGGTTATCTTACCACATGATATGAGCTTTATCAAACAAATGTTATGGTCCATATAATAATTGACTTGGATATAGATGAAGCTATTGATGTGATAGAGAGTGGATAGATCAATGATTATGTCAACGAAGGCTTGTTTGTCCTCTGTTTGAGGGGAGAATTGTCATGGGTGTAACCAGCAAGAAATATCATGAGTTTGTAAGGATAATATCTTTATTGATACAATACTTTTTGGATAGAATTCAAAAGTAAATTCAGTGGACAATATTTTTGCCGAGTACTAATATGGAGATACAAGAGATGGTACCTTGAGAGAGTTTTGAAAGTATAGATCCAAAAGGAAAATAAATGAGATTTCTTTCTATGGCTTCTGTGCTTGTGGCTTAGGGGATGGACTCTTTTACATCATCTAGAGGAGTGCGTCCTTGTAGCTTCATTGCCAGTGCTGCATCTTCTGCAAAAAGGCTTCAAATTTCTTGATTTATGATCGGGTTTCACATTTAAACAAATACAGAAGTCTCTTGTAAGCTTCATGGCCAATATAAGGGCGAGGATTATTTTGGAGTTACTTTGAAACTGTACATTTGAACTAACTTTGAGCCTTTTGAGGCAATGACTACTATATGGAGAACCTGGGCTCTGGATGCTCTATGAGACATATGGTTGAGAATAACCAGAAGATTTTAATAGATTTGTTTATGGGTGTTGGAGATTTGGAGATTTGCATTTTTATGTCCAAAAGCCAAAAAAAAAAAAAGGTAGAATGAGGTTAATTTATATGATGCTATGATGTACTTGTACTGGCAGGAAATGTTCTTGTTGTTTTGCTGGTCTTATGATTTCTATGAAGTTACTAACAGCCCCATCCATAACCAATATCATTATTGTTGCAGAAATTCTCACTGGATGGCCGTTTCTTAGTTAGTGCAGACCGTGATTTTAAAATTCGGGTAATCATATTCTCTCCTCATTTAATTATGATCAGTAATTAGTTGAAACTAATGTTTTTGAGAATGGTCTTGCTTTTTTGAAGGTTACAACGTTCCCTACAAAACCTGTGGATGAAGCTCATGAGATTCAAAGTTTTTGTCTAGGCCATACTGAGTGAGTTTCCATGATCTAAATCTATATATAGTACAAGATTAAAGTGGATAGTCAATAGTGGTGCTGTTTTTAAGTGACAATTCCTTGGTTTTTGATTCATATGTTTACATCATGTGTCTCCAGCTTTGTATCATGCGTAGCCTTCATTTCCAATCTGGAGTGCCCACATGGGTTTCTTATCTCTGGAAGTGGCGATTCAACTGTAAGACACTAATATGAACTCATATATTGTCTGCTTACTTGCTTTTGGTGCAATATGTGAAATATAATCCTATTTTGTTGCTTTTTTAGGTACGCTTATGGGATATTCTCTCTGGATCTCTCCTTGATATTTTCGAGATTGGGGATAAGGTGTTGGCCTCTAAACCCACACTTCAATTGTTTTAAGAAAATGGAATCTAAGAATCACAGATTTAATACTAGGCTTTCTCTCAAAATTATTACCTTGATTAATGAACACTCAAGCCATGAAAAGATACTTTTTATTTAGTTATTTATTTTATTTGAATGAAAAATCGAGCTTTCATCGAGGAAAAGGAAAGGATACAAAAGGCATACAATAAACCAGCCCAACAAAAGGAGCCAAACTAAAAGAACGAGCTCCAATCAAGCAAGATTAAACCTAAGGTGCAAGTAAAAAAATAACTTTGGACGTCAAGGCCCAAAGGTGATATGGAACCAAACAAGGTCCCACACCTCTCCCTCGATCTCTCTACCACTGAAAATCCTGTTGTTCTTTTCAAGCTACAAACCCCAAAGTACAGCAAAGAGATACTTGATAATTTAGACAAGGGTTTATTGTATACCATATATAGATTGGTGTAAAATATCTTTTGGTTGTCCACTTCTTACTCTGAAACTAGAAGAGTTAATTGAATTATGCATCATTTTCTTTTGTGGTATAGTTTTTGATAACCTGGTTCATTACTTGATTTCTCTTACTAATTTACTGAAGGTAGAACTTTTAGAGTCTAAAGAAAGAGAGGGGTGCAATTCGGCTGTCACAAGTCTCTGCACAATTGCTGATGGTCCCCTTGTTGCGGTGGCGGTTCAAAGGCAAATTTTCCTCTTCTTTTTCTCATTGTTCTCTTCTTTTTCTACTTTAATGGGATCCTACCATTGAACTTACCTTATCATTTCTCTTTGTAGCTTGCATGGAATAATTTTGCTGAACTGTGACCTGTCAAATCGTACTCTATCTGTAGTCAAGGTAAGGACTTTAATGCCAACTGAGCAATTCCAAAGGTTGTTGAAACAGGGTTCTCGAGATTATTATTTGACAAACTTATGGATGGCATATAATGACAAGATTTCATGCCCTCCACCCCCGCTTCCCGTAGACTTTTTGACGATGGAAGTAATATATCATCTTTTTTTGGTTTAATATTGGAGTGTCTGGATCATCTTATGTGCAGCCTTGACTAATTTTATTTAATTTTATTCTTCCCAAGATTAAATTCATTTGTTGTACTTTTGATAACTACAGGTGGTTTCTACAAGAGAGGAGACTTTCATCCCCACTAGCGTATCGAGCAGTTGCTCGACTGGATATCTATGGATGGTAACCGGTGTCTCCAGTTTGCATGACGTGGGGCAATCCTCTTTGGCCTGTGTCAAGGTCATCAGTGGTTTCAACAAGGATAATCCCTATTCCACTGGGAACGAGTTATTCGTACTGGAAGATGATGCAATACCTGGTGGAAAGAAGCTACTTGAAAAATTGCAGGGAGGTGTGTCGTTTGATGAAAAAGTATTCTTGGCAGCTGCTGAAGCTGTTAAGACTGCAATGAGCAATCTGTTGACTAAAAAACAGTACTCCACTGAGAAACGGGACTTCAGAAAGAGAACCAGAAATGATAGAAAACTTAAAAAATGATGGAGTATTAGTTCACTCGCACATCAGCAACAGGAGCCTGTTCTATGTAAAATTAGTTCTCTTTTTCAACATTTTTTAGCTTGACAGAGTCGCTTAGTTCCTTAGATTAATGTGATAATGTTTTAATTTATTCTGCAAGAAAAACTTGACGTGCTTTAGCCGCAGTTTTCTTTGTCAAAGCCATATCAGGAATAGGGATGTAAGTTGGTCCACTCCCCAATTATTTTGAAGAAATGACCAAAATAGCCCTGCCATCACAATTCTTGTTTGGTCTCTCTCTCTCCCATAGATGAAAACTGGGCCTGAAATGATTGAAGGTTCTTTTTATCCATGGAAACTGAATTTTGATGAAATATATGTTTAAATTGGTTTGTTATATGGCTTCAGGGGTAAGATTTCAGCCTCAGCTCAGCTCCAAGCAGGCAAAGGGGCAGAATTTGAAGGGTCAGGTAAGTTCATTAATTAGAAAATATGCAATTATTAGCCTATATGTTTGTAATGATTTGGAGGAATAATTAGTGGGATTTGAGATCAATCTACATCAGATGTCAATAATTGAGGTTGAGGACATATTAAAACAAGAGTGGGTGAAATTTAAAGAGAAAAAGACAGGCAAAAC

mRNA sequence

ATCTAAAACTAAAATTAAATTCCATGGTGTCATCTTTAGAAAACAAAACATTATAAAAAAAAATATACAAAGGGTATTTTGGTAAAAGTAACGTTTGAAATGGAACATTCCCCCCAAATTTTTCTCACGAAAACCCTAATTTTGCAGTTCAAGAAAACGCCCGTATTTCAGTTCACCGGAAGAATGGAGGAAACGCCGGTAGAGGAAGGAGATACCAACAAAGATGTCGAGGTTGCTCCGGCCTTAATTGCGGTTCACCCGGCCGAGAAATCTGTTGCCGTCGCCGTTGGGTCCGACCTGCGCGTATTCGATCTCGTAGGTAATTGTCCGGTTTCTTTGGTCGATGAATCTGGGGAACAGCATCATAAAGATTCCATTAGAGCTATTTGTTATGGTTCAAAAGGGCAGCTTTTTGTATCCGCTGGTGATGACAAAACTGTTAAGATTTGGTCGACTGAGTCTTGGCGCTGCATTTATAACGTGTGTTCTGAAAAACGAGTCAGTGCAGCTGCCATTAGTCCCGACGGGTTACATGTTTGTTTTGCTGACAAGTTTGGGGTGATTTGGGTTGTGGATCTTGATGGAGGGATTGATGAAAATCAACCTCTTGCCAACAAGACGGCTGTGTCGTTGCTTTCACATTATTGCAGTATAATCACTAGCTTGAAATTCTCACTGGATGGCCGTTTCTTAGTTAGTGCAGACCGTGATTTTAAAATTCGGGTTACAACGTTCCCTACAAAACCTGTGGATGAAGCTCATGAGATTCAAAGTTTTTGTCTAGGCCATACTGACTTTGTATCATGCGTAGCCTTCATTTCCAATCTGGAGTGCCCACATGGGTTTCTTATCTCTGGAAGTGGCGATTCAACTGTACGCTTATGGGATATTCTCTCTGGATCTCTCCTTGATATTTTCGAGATTGGGGATAAGGTAGAACTTTTAGAGTCTAAAGAAAGAGAGGGGTGCAATTCGGCTGTCACAAGTCTCTGCACAATTGCTGATGGTCCCCTTGTTGCGGTGGCGGTTCAAAGGCAAATTTTCCTCTTCTTTTTCTCATTCTTGCATGGAATAATTTTGCTGAACTGTGACCTGTCAAATCGTACTCTATCTGTAGTCAAGGTGGTTTCTACAAGAGAGGAGACTTTCATCCCCACTAGCGTATCGAGCAGTTGCTCGACTGGATATCTATGGATGGTAACCGGTGTCTCCAGTTTGCATGACGTGGGGCAATCCTCTTTGGCCTGTGTCAAGGTCATCAGTGGTTTCAACAAGGATAATCCCTATTCCACTGGGAACGAGTTATTCGTACTGGAAGATGATGCAATACCTGGTGGAAAGAAGCTACTTGAAAAATTGCAGGGAGGTGTGTCGTTTGATGAAAAAGTATTCTTGGCAGCTGCTGAAGCTGTTAAGACTGCAATGAGCAATCTGTTGACTAAAAAACAGTACTCCACTGAGAAACGGGACTTCAGAAAGAGAACCAGAAATGATAGAAAACTTAAAAAATGATGGAGTATTAGTTCACTCGCACATCAGCAACAGGAGCCTGTTCTATGTAAAATTAGTTCTCTTTTTCAACATTTTTTAGCTTGACAGAGTCGCTTAGTTCCTTAGATTAATGTGATAATGTTTTAATTTATTCTGCAAGAAAAACTTGACGTGCTTTAGCCGCAGTTTTCTTTGTCAAAGCCATATCAGGAATAGGGATGTAAGTTGGTCCACTCCCCAATTATTTTGAAGAAATGACCAAAATAGCCCTGCCATCACAATTCTTGTTTGGTCTCTCTCTCTCCCATAGATGAAAACTGGGCCTGAAATGATTGAAGGTTCTTTTTATCCATGGAAACTGAATTTTGATGAAATATATGTTTAAATTGGTTTGTTATATGGCTTCAGGGGTAAGATTTCAGCCTCAGCTCAGCTCCAAGCAGGCAAAGGGGCAGAATTTGAAGGGTCAGGTAAGTTCATTAATTAGAAAATATGCAATTATTAGCCTATATGTTTGTAATGATTTGGAGGAATAATTAGTGGGATTTGAGATCAATCTACATCAGATGTCAATAATTGAGGTTGAGGACATATTAAAACAAGAGTGGGTGAAATTTAAAGAGAAAAAGACAGGCAAAAC

Coding sequence (CDS)

ATGGAACATTCCCCCCAAATTTTTCTCACGAAAACCCTAATTTTGCAGTTCAAGAAAACGCCCGTATTTCAGTTCACCGGAAGAATGGAGGAAACGCCGGTAGAGGAAGGAGATACCAACAAAGATGTCGAGGTTGCTCCGGCCTTAATTGCGGTTCACCCGGCCGAGAAATCTGTTGCCGTCGCCGTTGGGTCCGACCTGCGCGTATTCGATCTCGTAGGTAATTGTCCGGTTTCTTTGGTCGATGAATCTGGGGAACAGCATCATAAAGATTCCATTAGAGCTATTTGTTATGGTTCAAAAGGGCAGCTTTTTGTATCCGCTGGTGATGACAAAACTGTTAAGATTTGGTCGACTGAGTCTTGGCGCTGCATTTATAACGTGTGTTCTGAAAAACGAGTCAGTGCAGCTGCCATTAGTCCCGACGGGTTACATGTTTGTTTTGCTGACAAGTTTGGGGTGATTTGGGTTGTGGATCTTGATGGAGGGATTGATGAAAATCAACCTCTTGCCAACAAGACGGCTGTGTCGTTGCTTTCACATTATTGCAGTATAATCACTAGCTTGAAATTCTCACTGGATGGCCGTTTCTTAGTTAGTGCAGACCGTGATTTTAAAATTCGGGTTACAACGTTCCCTACAAAACCTGTGGATGAAGCTCATGAGATTCAAAGTTTTTGTCTAGGCCATACTGACTTTGTATCATGCGTAGCCTTCATTTCCAATCTGGAGTGCCCACATGGGTTTCTTATCTCTGGAAGTGGCGATTCAACTGTACGCTTATGGGATATTCTCTCTGGATCTCTCCTTGATATTTTCGAGATTGGGGATAAGGTAGAACTTTTAGAGTCTAAAGAAAGAGAGGGGTGCAATTCGGCTGTCACAAGTCTCTGCACAATTGCTGATGGTCCCCTTGTTGCGGTGGCGGTTCAAAGGCAAATTTTCCTCTTCTTTTTCTCATTCTTGCATGGAATAATTTTGCTGAACTGTGACCTGTCAAATCGTACTCTATCTGTAGTCAAGGTGGTTTCTACAAGAGAGGAGACTTTCATCCCCACTAGCGTATCGAGCAGTTGCTCGACTGGATATCTATGGATGGTAACCGGTGTCTCCAGTTTGCATGACGTGGGGCAATCCTCTTTGGCCTGTGTCAAGGTCATCAGTGGTTTCAACAAGGATAATCCCTATTCCACTGGGAACGAGTTATTCGTACTGGAAGATGATGCAATACCTGGTGGAAAGAAGCTACTTGAAAAATTGCAGGGAGGTGTGTCGTTTGATGAAAAAGTATTCTTGGCAGCTGCTGAAGCTGTTAAGACTGCAATGAGCAATCTGTTGACTAAAAAACAGTACTCCACTGAGAAACGGGACTTCAGAAAGAGAACCAGAAATGATAGAAAACTTAAAAAATGA

Protein sequence

MEHSPQIFLTKTLILQFKKTPVFQFTGRMEETPVEEGDTNKDVEVAPALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLVDESGEQHHKDSIRAICYGSKGQLFVSAGDDKTVKIWSTESWRCIYNVCSEKRVSAAAISPDGLHVCFADKFGVIWVVDLDGGIDENQPLANKTAVSLLSHYCSIITSLKFSLDGRFLVSADRDFKIRVTTFPTKPVDEAHEIQSFCLGHTDFVSCVAFISNLECPHGFLISGSGDSTVRLWDILSGSLLDIFEIGDKVELLESKEREGCNSAVTSLCTIADGPLVAVAVQRQIFLFFFSFLHGIILLNCDLSNRTLSVVKVVSTREETFIPTSVSSSCSTGYLWMVTGVSSLHDVGQSSLACVKVISGFNKDNPYSTGNELFVLEDDAIPGGKKLLEKLQGGVSFDEKVFLAAAEAVKTAMSNLLTKKQYSTEKRDFRKRTRNDRKLKK
Homology
BLAST of MC09g0727 vs. ExPASy Swiss-Prot
Match: Q54UI3 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 OS=Dictyostelium discoideum OX=44689 GN=wdr4 PE=3 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 6.6e-23
Identity = 80/257 (31.13%), Postives = 131/257 (50.97%), Query Frame = 0

Query: 42  DVEVAPALIAVHPAEKS-VAVAVGSDLRVFDLVGNCPVSLVDESGEQHHKDSIRAICYGS 101
           +VE  P  I  H ++ + +A  + + L +FD   N    L  ESG Q H   IR+I +  
Sbjct: 17  NVEKVPISIITHSSDSNFIAYRLNNKLNIFDNKLNKLGEL--ESGSQ-HTGIIRSIEFTK 76

Query: 102 KGQLFVSAGDDKTVKIW-STESWRCIYNVCSEKRVSAAAISPDGLHVCFADKFGVIWVVD 161
             Q  +++  DK +KIW +T +++ I ++ + K++  + ++ D   +  +DK G ++   
Sbjct: 77  NNQSLITSSSDKFLKIWDTTNNFKNIKSINTNKKIICSILNKDDSEILVSDKCGDVFKYS 136

Query: 162 LDGGIDENQPLANKTAVS----------------LLSHYCSIITSLKFSLDGRFLVSADR 221
           L   ID+++   NK  VS                +  HY SI+  +KFS    +L+SADR
Sbjct: 137 L---IDDSK---NKIEVSGDKSAKHDEKESDKNLVFGHYSSIV-DIKFSPCFNYLLSADR 196

Query: 222 DFKIRVTTFPTKPVDEAHEIQSFCLGHTDFVSCVAFISNLECPHGFLISGSGDSTVRLWD 281
           D KIRV+ +P        +I+SFCLGHT +V+ +  +   +     LISGSGD T++LW+
Sbjct: 197 DEKIRVSHYP-----NCFDIESFCLGHTKYVTEILLVPGRD---DLLISGSGDGTIKLWN 255

BLAST of MC09g0727 vs. ExPASy Swiss-Prot
Match: Q7ZY78 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 OS=Xenopus laevis OX=8355 GN=wdr4 PE=2 SV=2)

HSP 1 Score: 105.9 bits (263), Expect = 1.3e-21
Identity = 82/288 (28.47%), Postives = 137/288 (47.57%), Query Frame = 0

Query: 43  VEVAPALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLVDE---------SGEQHHKDSI 102
           + V P  +A+    + +   VGS+   F L  +C  SL+++         S + H  D I
Sbjct: 2   LRVGPGSLAITGGSRLLGHRVGSECCPFHL--DC--SLLEKQSAAPGQEGSADSHGSDKI 61

Query: 103 RAICYGSKGQLFVSAGDDKTVKIWSTE-SWRCIYNVCSEKRVSAAAISPDGLHVCFADKF 162
            A  +   G+ F    D+K + ++ T+ +W  I      +R +A   SP G H+  ADK 
Sbjct: 62  LAAAFSPSGEYFALTDDNKRLVLFRTKPAWEKISVRWVSRRCTALTFSPCGNHILVADKS 121

Query: 163 GVIWVVDLDGGIDENQPLANKTAVSLLSHYCSIITSLKFSLDGRFLVSADRDFKIRVTTF 222
           G ++   +   +++ +         L   + S++  +  SLDG+ +++ DRD KIRV+ +
Sbjct: 122 GDVFSFSVPRALEQGR---------LELGHLSMLLDVTVSLDGKHIITCDRDEKIRVSCW 181

Query: 223 PTKPVDEAHEIQSFCLGHTDFVSCVAFISNLECPHGFLISGSGDSTVRLWDILSGSLLDI 282
                   H I SFCLGHT+FVS +  +   E     L+SGSGD T+RLW+  SG  +  
Sbjct: 182 -----GAPHVIMSFCLGHTEFVSQLLPLPGQE---KLLLSGSGDGTLRLWEYESGKEVHS 241

Query: 283 FEIGDKVELLESKEREGCNSAVTSLCTIADGPLVAVAVQRQIFLFFFS 321
             +      LE +E +    +  S C+  +G  +AV  +    +F FS
Sbjct: 242 VTLRSLAHELEDQENKRFAVSRISCCS-CNGIQLAVLCEGVPGIFLFS 267

BLAST of MC09g0727 vs. ExPASy Swiss-Prot
Match: Q5FVB6 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 OS=Xenopus tropicalis OX=8364 GN=wdr4 PE=2 SV=2)

HSP 1 Score: 95.9 bits (237), Expect = 1.3e-18
Identity = 69/235 (29.36%), Postives = 111/235 (47.23%), Query Frame = 0

Query: 91  DSIRAICYGSKGQLFVSAGDDKTVKIWSTE-SWRCIYNVCSEKRVSAAAISPDGLHVCFA 150
           D I A  +   G+ F    D+K + ++ T+ +W  I      +R +A   SP G H+  A
Sbjct: 55  DKILAAAFSPSGEYFALTDDNKRLILFQTKPAWETISVRWVSRRCTALTFSPCGAHILVA 114

Query: 151 DKFGVIWVVDLDGGIDENQPLANKTAVSLLSHYCSIITSLKFSLDGRFLVSADRDFKIRV 210
           DK G ++   +           ++ A  L   + S++  +  S DG  +++ DRD KIRV
Sbjct: 115 DKSGDVFSFSV---------RRSREAGRLELGHLSMLLDVAVSPDGNHIITCDRDEKIRV 174

Query: 211 TTFPTKPVDEAHEIQSFCLGHTDFVSCVAFISNLECPHGFLISGSGDSTVRLWDILSGSL 270
           + +     D  H I SFCLGHT+FVS +  +   +     L+SGSGD T+RLWD  SG  
Sbjct: 175 SRW-----DSPHVIMSFCLGHTEFVSQLLPLPGAD---KLLLSGSGDGTLRLWDYESGRE 234

Query: 271 LDIFEIGDKVELLESKEREGCNSAVTSLCTIADGPLVAVAVQRQIFLFFFSFLHG 325
           +    +      +E +E +    +  S C   +G  +A+  +    +F FS   G
Sbjct: 235 VQSVTLSGLDLGMERQENKRFTVSRISCCA-CNGIQLAMLCEGVPGIFLFSVAPG 271

BLAST of MC09g0727 vs. ExPASy Swiss-Prot
Match: A4IGH4 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 OS=Danio rerio OX=7955 GN=wdr4 PE=2 SV=2)

HSP 1 Score: 90.5 bits (223), Expect = 5.4e-17
Identity = 74/233 (31.76%), Postives = 112/233 (48.07%), Query Frame = 0

Query: 83  ESGEQHHKDSIRAICYGSKGQLFVSAGDDKTVKIWSTE-SWRCIYNVCSEKRVSAAAISP 142
           E  E+   DSI A    + G+      D K + ++ TE SW+CI      +R ++ A   
Sbjct: 54  EGSEEKDSDSILAFAISASGKHVALTDDHKRLVLFCTEPSWKCISTRWVVRRCTSLA--- 113

Query: 143 DGLHVCFADKFGVIWVVDLDGGIDENQPLANKTAVSLLSHYCSIITSLKFSLDGRFLVSA 202
                 F      ++V D  G +     L    A  L   + S++  +  S D +++++A
Sbjct: 114 ------FTQAEDELYVADKSGDVYSFSILEPHKAGELKLGHLSMLLDVALSPDDKYIITA 173

Query: 203 DRDFKIRVTTFPTKPVDEAHEIQSFCLGHTDFVSCVAFISNLECPHG---FLISGSGDST 262
           DRD KIRV +F   P    + IQ+FCLGHT+FV      S+L  P G   +L+SGSGD T
Sbjct: 174 DRDEKIRV-SFRRSP----YNIQAFCLGHTEFV------SSLLVPAGHPDWLLSGSGDGT 233

Query: 263 VRLWDILSGSLL---DIFEIGDKVELLESKEREGCNSAVTSLCTIADGPLVAV 309
           V +W   +G  L   D+ + G     L+S+  E    AV+ + +  DG  VAV
Sbjct: 234 VNVWHYETGRRLHSVDMRKFG-----LDSENTEK-RFAVSRIISSPDGQHVAV 260

BLAST of MC09g0727 vs. ExPASy Swiss-Prot
Match: P57081 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4 OS=Homo sapiens OX=9606 GN=WDR4 PE=1 SV=2)

HSP 1 Score: 89.7 bits (221), Expect = 9.3e-17
Identity = 59/192 (30.73%), Postives = 92/192 (47.92%), Query Frame = 0

Query: 92  SIRAICYGSKGQLFVSAGDDKTVKIWSTESWRCIYNVCSEKRVSAAAISPDGLHVCFADK 151
           +I A  +   G  F    D K + ++ T+ W+C+      +R +A         V  ADK
Sbjct: 63  AILASTFSKSGSYFALTDDSKRLILFRTKPWQCLSVRTVARRCTALTFIASEEKVLVADK 122

Query: 152 FGVIWVVDLDGGIDENQPLANKTAVSLLSHYCSIITSLKFSLDGRFLVSADRDFKIRVTT 211
            G ++   +         L       L   + S++  +  S D RF+++ADRD KIRV +
Sbjct: 123 SGDVYSFSV---------LEPHGCGRLELGHLSMLLDVAVSPDDRFILTADRDEKIRV-S 182

Query: 212 FPTKPVDEAHEIQSFCLGHTDFVSCVAFISNLECPHGFLISGSGDSTVRLWDILSGSLLD 271
           +   P    H I+SFCLGHT+FVS ++ +       G L+S SGD T+RLW+  SG  L 
Sbjct: 183 WAAAP----HSIESFCLGHTEFVSRISVVPT---QPGLLLSSSGDGTLRLWEYRSGRQLH 237

Query: 272 IFEIGDKVELLE 284
              +    EL++
Sbjct: 243 CCHLASLQELVD 237

BLAST of MC09g0727 vs. NCBI nr
Match: XP_022150294.1 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 [Momordica charantia])

HSP 1 Score: 849 bits (2193), Expect = 1.04e-309
Identity = 432/442 (97.74%), Postives = 432/442 (97.74%), Query Frame = 0

Query: 29  MEETPVEEGDTNKDVEVAPALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLVDESGEQH 88
           MEETPVEEGDTNKDVEVAPALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLVDESGEQH
Sbjct: 1   MEETPVEEGDTNKDVEVAPALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLVDESGEQH 60

Query: 89  HKDSIRAICYGSKGQLFVSAGDDKTVKIWSTESWRCIYNVCSEKRVSAAAISPDGLHVCF 148
           HKDSIRAICYGSKGQLFVSAGDDKTVKIWSTESWRCIYNVCSEKRVSAAAISPDGLHVCF
Sbjct: 61  HKDSIRAICYGSKGQLFVSAGDDKTVKIWSTESWRCIYNVCSEKRVSAAAISPDGLHVCF 120

Query: 149 ADKFGVIWVVDLDGGIDENQPLANKTAVSLLSHYCSIITSLKFSLDGRFLVSADRDFKIR 208
           ADKFGVIWVVDLDGGIDENQPLANKTAVSLLSHYCSIITSLKFSLDGRFLVSADRDFKIR
Sbjct: 121 ADKFGVIWVVDLDGGIDENQPLANKTAVSLLSHYCSIITSLKFSLDGRFLVSADRDFKIR 180

Query: 209 VTTFPTKPVDEAHEIQSFCLGHTDFVSCVAFISNLECPHGFLISGSGDSTVRLWDILSGS 268
           VTTFPTKPVDEAHEIQSFCLGHTDFVSCVAFISNLECPHGFLISGSGDSTVRLWDILSGS
Sbjct: 181 VTTFPTKPVDEAHEIQSFCLGHTDFVSCVAFISNLECPHGFLISGSGDSTVRLWDILSGS 240

Query: 269 LLDIFEIGDKVELLESKEREGCNSAVTSLCTIADGPLVAVAVQRQIFLFFFSFLHGIILL 328
           LLDIFEIGDKVELLESKEREGCNSAVTSLCTIADGPLVAVAVQ          LHGIILL
Sbjct: 241 LLDIFEIGDKVELLESKEREGCNSAVTSLCTIADGPLVAVAVQS---------LHGIILL 300

Query: 329 NCDLSNRTLSVVKVVSTREETFIPTSVSSSCSTGYLWMVTGVSSLHDVGQSSLACVKVIS 388
           NCDLSNRTLSVVKVVSTREETFIPTSVSSSCSTGYLWMVTGVSSLHDVGQSSLACVKVIS
Sbjct: 301 NCDLSNRTLSVVKVVSTREETFIPTSVSSSCSTGYLWMVTGVSSLHDVGQSSLACVKVIS 360

Query: 389 GFNKDNPYSTGNELFVLEDDAIPGGKKLLEKLQGGVSFDEKVFLAAAEAVKTAMSNLLTK 448
           GFNKDNPYSTGNELFVLEDDAIPGGKKLLEKLQGGVSFDEKVFLAAAEAVKTAMSNLLTK
Sbjct: 361 GFNKDNPYSTGNELFVLEDDAIPGGKKLLEKLQGGVSFDEKVFLAAAEAVKTAMSNLLTK 420

Query: 449 KQYSTEKRDFRKRTRNDRKLKK 470
           KQYSTEKRDFRKRTRNDRKLKK
Sbjct: 421 KQYSTEKRDFRKRTRNDRKLKK 433

BLAST of MC09g0727 vs. NCBI nr
Match: XP_023549061.1 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 [Cucurbita pepo subsp. pepo] >XP_023549063.1 tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 764 bits (1973), Expect = 3.56e-276
Identity = 388/441 (87.98%), Postives = 408/441 (92.52%), Query Frame = 0

Query: 29  MEETPVEEGDTNKDVEVAPALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLVDESGEQH 88
           MEETPVEEGDTNKDVE+APALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLVDESG  +
Sbjct: 1   MEETPVEEGDTNKDVEIAPALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLVDESGGSY 60

Query: 89  HKDSIRAICYGSKGQLFVSAGDDKTVKIWSTESWRCIYNVCSEKRVSAAAISPDGLHVCF 148
           HKDSIR ICYGS GQLFVSAGDDKTVKIWSTESWRCIYNV SEKRVSAAAISP+G+HVCF
Sbjct: 61  HKDSIRTICYGSNGQLFVSAGDDKTVKIWSTESWRCIYNVGSEKRVSAAAISPNGMHVCF 120

Query: 149 ADKFGVIWVVDLDGGIDENQPLANKTAVSLLSHYCSIITSLKFSLDGRFLVSADRDFKIR 208
           ADKFGVIWVVDLDGG+D NQPL NK AVSLLSHYCSIITSL+FSLDGRFLVSADRDFKIR
Sbjct: 121 ADKFGVIWVVDLDGGVDGNQPLVNKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIR 180

Query: 209 VTTFPTKPVDEAHEIQSFCLGHTDFVSCVAFISNLECPHGFLISGSGDSTVRLWDILSGS 268
           VTTFPTKPV+EAHEIQSFCLGHTDFVSC+AFIS LECPHGF+ISGSGDSTVRLWDILSGS
Sbjct: 181 VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISKLECPHGFIISGSGDSTVRLWDILSGS 240

Query: 269 LLDIFEIGDKVELLESKEREGCNSAVTSLCTIADGPLVAVAVQRQIFLFFFSFLHGIILL 328
           LL   EIGDKVELL+SKEREGC+SAVTS+CTIADG LVAVA+Q          LHGIILL
Sbjct: 241 LLATCEIGDKVELLDSKEREGCHSAVTSICTIADGGLVAVAIQS---------LHGIILL 300

Query: 329 NCDLSNRTLSVVKVVSTREETFIPTSVSSSCSTGYLWMVTGVSSLHDVGQSSLACVKVIS 388
           NCDLSN TLSVVKVVS  EETFIPTS+SSSCST YLWMVTGVS LH++G SSLA VKVIS
Sbjct: 301 NCDLSNHTLSVVKVVSISEETFIPTSLSSSCSTRYLWMVTGVSGLHNLGHSSLARVKVIS 360

Query: 389 GFNKDNPYSTGNELFVLEDDAIPGGKKLLEKLQGGVSFDEKVFLAAAEAVKTAMSNLLTK 448
           GFNKDNP ST NEL +LEDDAIPGG+KLLEKLQGGV+FDEKVFLAA+EAVKTAMSNLLTK
Sbjct: 361 GFNKDNPNSTENELSILEDDAIPGGEKLLEKLQGGVTFDEKVFLAASEAVKTAMSNLLTK 420

Query: 449 KQYSTEKRDFRKRTRNDRKLK 469
           KQYSTEKRDFRKRTRNDRKLK
Sbjct: 421 KQYSTEKRDFRKRTRNDRKLK 432

BLAST of MC09g0727 vs. NCBI nr
Match: KAG7014455.1 (F-box/kelch-repeat protein SKIP30 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 777 bits (2007), Expect = 1.35e-274
Identity = 398/465 (85.59%), Postives = 417/465 (89.68%), Query Frame = 0

Query: 25  FTGRMEETPVEEGDTNKDVEVAPALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLVDES 84
           FTGRMEETPVEEGDTNKDVE+APALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLVDES
Sbjct: 404 FTGRMEETPVEEGDTNKDVEIAPALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLVDES 463

Query: 85  GEQHHKDSIRAICYGSKGQLFVSAGDDKTVKIWSTESWRCIYNVCSEKRVSAAAISPDGL 144
           G  +HKDSIR ICYGS GQLFVSAGDDKTVKIWSTESWRCIYNV SEKRVSAAAISP+G+
Sbjct: 464 GGSYHKDSIRTICYGSNGQLFVSAGDDKTVKIWSTESWRCIYNVGSEKRVSAAAISPNGM 523

Query: 145 HVCFADKFGVIWVVDLDGGIDENQPLANKTAVSLLSHYCSIITSLKFSLDGRFLVSADRD 204
           HVCFADKFGVIWVVDLDGG+D NQPL NK AVSLLSHYCSIITSL+FSLDGRFLVSADRD
Sbjct: 524 HVCFADKFGVIWVVDLDGGVDGNQPLVNKKAVSLLSHYCSIITSLEFSLDGRFLVSADRD 583

Query: 205 FKIRVTTFPTKPVDEAHEIQSFCLGHTDFVSCVAFISNLECPHGFLISGSGDSTVRLWDI 264
           FKIRVTTFPTKPV+EAHEIQSFCLGHTDFVSC+AFIS LECPHGFLISGSGDSTVRLWDI
Sbjct: 584 FKIRVTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISKLECPHGFLISGSGDSTVRLWDI 643

Query: 265 LSGSLLDIFEIGDKVELLESKEREGCNSAVTSLCTIADGPLVAVAVQRQI---------- 324
           LSGSLL   EIGDKVELL+SKEREGC+SAVTS+CTIADG LVAVA+QRQ           
Sbjct: 644 LSGSLLATCEIGDKVELLDSKEREGCHSAVTSICTIADGGLVAVAIQRQFSHLCSSSLVE 703

Query: 325 ----------FLFFFSFLHGIILLNCDLSNRTLSVVKVVSTREETFIPTSVSSSCSTGYL 384
                     F+ F   LHGIILLNCDLSN TLSVVKVVS  EETFIPTS+SSSCST YL
Sbjct: 704 SYHRIFYLIYFIIFLCSLHGIILLNCDLSNHTLSVVKVVSISEETFIPTSLSSSCSTRYL 763

Query: 385 WMVTGVSSLHDVGQSSLACVKVISGFNKDNPYSTGNELFVLEDDAIPGGKKLLEKLQGGV 444
           WMVTGVS LH++G SSLA VKVISGFNKDNP ST NEL +LEDDAIPGG+KLLEKLQGGV
Sbjct: 764 WMVTGVSGLHNLGHSSLARVKVISGFNKDNPNSTENELSILEDDAIPGGEKLLEKLQGGV 823

Query: 445 SFDEKVFLAAAEAVKTAMSNLLTKKQYSTEKRDFRKRTRNDRKLK 469
           +FDEKVFLAAAEAVKTAMSNLLTKKQYSTEKRDFRKRTRNDRKLK
Sbjct: 824 TFDEKVFLAAAEAVKTAMSNLLTKKQYSTEKRDFRKRTRNDRKLK 868

BLAST of MC09g0727 vs. NCBI nr
Match: XP_022954128.1 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 [Cucurbita moschata] >XP_022954129.1 tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 [Cucurbita moschata])

HSP 1 Score: 760 bits (1962), Expect = 1.68e-274
Identity = 386/441 (87.53%), Postives = 406/441 (92.06%), Query Frame = 0

Query: 29  MEETPVEEGDTNKDVEVAPALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLVDESGEQH 88
           MEETPVEEGDTNKDVE+APALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSL+DESG  +
Sbjct: 1   MEETPVEEGDTNKDVEIAPALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLIDESGGSY 60

Query: 89  HKDSIRAICYGSKGQLFVSAGDDKTVKIWSTESWRCIYNVCSEKRVSAAAISPDGLHVCF 148
           HKDSIR ICYGS GQLFVSAGDDKTVKIWSTESWRCIYNV SEKRVSAAAISP+G+HVCF
Sbjct: 61  HKDSIRTICYGSNGQLFVSAGDDKTVKIWSTESWRCIYNVGSEKRVSAAAISPNGMHVCF 120

Query: 149 ADKFGVIWVVDLDGGIDENQPLANKTAVSLLSHYCSIITSLKFSLDGRFLVSADRDFKIR 208
           ADKFGVIWVVDLDGG+D NQPL NK AVSLLSHYCSIITSL+FSLDGRFLVSADRDFKIR
Sbjct: 121 ADKFGVIWVVDLDGGVDGNQPLVNKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIR 180

Query: 209 VTTFPTKPVDEAHEIQSFCLGHTDFVSCVAFISNLECPHGFLISGSGDSTVRLWDILSGS 268
           VTTFPTKPV+EAHEIQSFCLGHTDFVSC+AFIS LECPHGFLISGSGDSTVRLWDILSGS
Sbjct: 181 VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISKLECPHGFLISGSGDSTVRLWDILSGS 240

Query: 269 LLDIFEIGDKVELLESKEREGCNSAVTSLCTIADGPLVAVAVQRQIFLFFFSFLHGIILL 328
           LL   E+GDKVELLESKEREGC+SAVTS+CTI DG LVAVA+Q          LHGIILL
Sbjct: 241 LLATCEVGDKVELLESKEREGCHSAVTSICTITDGALVAVAIQS---------LHGIILL 300

Query: 329 NCDLSNRTLSVVKVVSTREETFIPTSVSSSCSTGYLWMVTGVSSLHDVGQSSLACVKVIS 388
           NCDLSN TLSVVKVVST EETFIPTS+SSS ST YLWMVTGVS LH++G +SLA VKVIS
Sbjct: 301 NCDLSNHTLSVVKVVSTSEETFIPTSLSSSFSTRYLWMVTGVSGLHNLGHASLARVKVIS 360

Query: 389 GFNKDNPYSTGNELFVLEDDAIPGGKKLLEKLQGGVSFDEKVFLAAAEAVKTAMSNLLTK 448
           GFNKDNP  T NEL +LEDDAIPGG+KLLEKLQGGV+FDEKVFLAAAEAVKTAMSNLLTK
Sbjct: 361 GFNKDNPNFTENELSILEDDAIPGGEKLLEKLQGGVTFDEKVFLAAAEAVKTAMSNLLTK 420

Query: 449 KQYSTEKRDFRKRTRNDRKLK 469
           KQYSTEKRDFRKRTRNDRKLK
Sbjct: 421 KQYSTEKRDFRKRTRNDRKLK 432

BLAST of MC09g0727 vs. NCBI nr
Match: XP_022991957.1 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 [Cucurbita maxima] >XP_022991958.1 tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 [Cucurbita maxima])

HSP 1 Score: 753 bits (1943), Expect = 1.27e-271
Identity = 385/441 (87.30%), Postives = 404/441 (91.61%), Query Frame = 0

Query: 29  MEETPVEEGDTNKDVEVAPALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLVDESGEQH 88
           MEETPVE GDT KDVE+APALIAVHPAEKSVAVAVGSDLRVFDLV NCPVSL+DESG  +
Sbjct: 1   MEETPVE-GDTKKDVEIAPALIAVHPAEKSVAVAVGSDLRVFDLVDNCPVSLIDESGGSY 60

Query: 89  HKDSIRAICYGSKGQLFVSAGDDKTVKIWSTESWRCIYNVCSEKRVSAAAISPDGLHVCF 148
           HKDSIR ICYGS GQLFVSAGDDKTVKIWSTESWRCIYNV SEKRVSAAAISP+G+HVCF
Sbjct: 61  HKDSIRTICYGSNGQLFVSAGDDKTVKIWSTESWRCIYNVGSEKRVSAAAISPNGMHVCF 120

Query: 149 ADKFGVIWVVDLDGGIDENQPLANKTAVSLLSHYCSIITSLKFSLDGRFLVSADRDFKIR 208
           ADKFGVIWVVDLDGG+D NQPL NK AVSLLSHYCSIITSL+FSLDGRFLVSADRDFKIR
Sbjct: 121 ADKFGVIWVVDLDGGVDGNQPLVNKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIR 180

Query: 209 VTTFPTKPVDEAHEIQSFCLGHTDFVSCVAFISNLECPHGFLISGSGDSTVRLWDILSGS 268
           VTTFPTKPV+EAHEIQSFCLGHTDFVSC+AFIS LECPHGFLISGSGDSTVRLWDILSGS
Sbjct: 181 VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISKLECPHGFLISGSGDSTVRLWDILSGS 240

Query: 269 LLDIFEIGDKVELLESKEREGCNSAVTSLCTIADGPLVAVAVQRQIFLFFFSFLHGIILL 328
           LL   EIGDKVELLESKEREGC+SAVTS+CTIADG LVAVA+Q          LHGIILL
Sbjct: 241 LLATCEIGDKVELLESKEREGCHSAVTSICTIADGTLVAVAIQS---------LHGIILL 300

Query: 329 NCDLSNRTLSVVKVVSTREETFIPTSVSSSCSTGYLWMVTGVSSLHDVGQSSLACVKVIS 388
           NCDLSN +LSVVKVVS  EETFIPTS+SSSCST YLWMVTGVS LH++G SSL  VKVIS
Sbjct: 301 NCDLSNHSLSVVKVVSISEETFIPTSLSSSCSTRYLWMVTGVSGLHNLGHSSLVRVKVIS 360

Query: 389 GFNKDNPYSTGNELFVLEDDAIPGGKKLLEKLQGGVSFDEKVFLAAAEAVKTAMSNLLTK 448
           GFNKDNP ST NEL +LEDDAIPGG+KLLEKLQGGV+FDEKVFLAAAEAVKTAMSNLLTK
Sbjct: 361 GFNKDNPNSTENELSILEDDAIPGGEKLLEKLQGGVTFDEKVFLAAAEAVKTAMSNLLTK 420

Query: 449 KQYSTEKRDFRKRTRNDRKLK 469
           KQYSTEKRDFRKRTRNDRKLK
Sbjct: 421 KQYSTEKRDFRKRTRNDRKLK 431

BLAST of MC09g0727 vs. ExPASy TrEMBL
Match: A0A6J1D9K9 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit OS=Momordica charantia OX=3673 GN=LOC111018494 PE=3 SV=1)

HSP 1 Score: 849 bits (2193), Expect = 5.05e-310
Identity = 432/442 (97.74%), Postives = 432/442 (97.74%), Query Frame = 0

Query: 29  MEETPVEEGDTNKDVEVAPALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLVDESGEQH 88
           MEETPVEEGDTNKDVEVAPALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLVDESGEQH
Sbjct: 1   MEETPVEEGDTNKDVEVAPALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLVDESGEQH 60

Query: 89  HKDSIRAICYGSKGQLFVSAGDDKTVKIWSTESWRCIYNVCSEKRVSAAAISPDGLHVCF 148
           HKDSIRAICYGSKGQLFVSAGDDKTVKIWSTESWRCIYNVCSEKRVSAAAISPDGLHVCF
Sbjct: 61  HKDSIRAICYGSKGQLFVSAGDDKTVKIWSTESWRCIYNVCSEKRVSAAAISPDGLHVCF 120

Query: 149 ADKFGVIWVVDLDGGIDENQPLANKTAVSLLSHYCSIITSLKFSLDGRFLVSADRDFKIR 208
           ADKFGVIWVVDLDGGIDENQPLANKTAVSLLSHYCSIITSLKFSLDGRFLVSADRDFKIR
Sbjct: 121 ADKFGVIWVVDLDGGIDENQPLANKTAVSLLSHYCSIITSLKFSLDGRFLVSADRDFKIR 180

Query: 209 VTTFPTKPVDEAHEIQSFCLGHTDFVSCVAFISNLECPHGFLISGSGDSTVRLWDILSGS 268
           VTTFPTKPVDEAHEIQSFCLGHTDFVSCVAFISNLECPHGFLISGSGDSTVRLWDILSGS
Sbjct: 181 VTTFPTKPVDEAHEIQSFCLGHTDFVSCVAFISNLECPHGFLISGSGDSTVRLWDILSGS 240

Query: 269 LLDIFEIGDKVELLESKEREGCNSAVTSLCTIADGPLVAVAVQRQIFLFFFSFLHGIILL 328
           LLDIFEIGDKVELLESKEREGCNSAVTSLCTIADGPLVAVAVQ          LHGIILL
Sbjct: 241 LLDIFEIGDKVELLESKEREGCNSAVTSLCTIADGPLVAVAVQS---------LHGIILL 300

Query: 329 NCDLSNRTLSVVKVVSTREETFIPTSVSSSCSTGYLWMVTGVSSLHDVGQSSLACVKVIS 388
           NCDLSNRTLSVVKVVSTREETFIPTSVSSSCSTGYLWMVTGVSSLHDVGQSSLACVKVIS
Sbjct: 301 NCDLSNRTLSVVKVVSTREETFIPTSVSSSCSTGYLWMVTGVSSLHDVGQSSLACVKVIS 360

Query: 389 GFNKDNPYSTGNELFVLEDDAIPGGKKLLEKLQGGVSFDEKVFLAAAEAVKTAMSNLLTK 448
           GFNKDNPYSTGNELFVLEDDAIPGGKKLLEKLQGGVSFDEKVFLAAAEAVKTAMSNLLTK
Sbjct: 361 GFNKDNPYSTGNELFVLEDDAIPGGKKLLEKLQGGVSFDEKVFLAAAEAVKTAMSNLLTK 420

Query: 449 KQYSTEKRDFRKRTRNDRKLKK 470
           KQYSTEKRDFRKRTRNDRKLKK
Sbjct: 421 KQYSTEKRDFRKRTRNDRKLKK 433

BLAST of MC09g0727 vs. ExPASy TrEMBL
Match: A0A6J1GQ89 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit OS=Cucurbita moschata OX=3662 GN=LOC111456486 PE=3 SV=1)

HSP 1 Score: 760 bits (1962), Expect = 8.15e-275
Identity = 386/441 (87.53%), Postives = 406/441 (92.06%), Query Frame = 0

Query: 29  MEETPVEEGDTNKDVEVAPALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLVDESGEQH 88
           MEETPVEEGDTNKDVE+APALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSL+DESG  +
Sbjct: 1   MEETPVEEGDTNKDVEIAPALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLIDESGGSY 60

Query: 89  HKDSIRAICYGSKGQLFVSAGDDKTVKIWSTESWRCIYNVCSEKRVSAAAISPDGLHVCF 148
           HKDSIR ICYGS GQLFVSAGDDKTVKIWSTESWRCIYNV SEKRVSAAAISP+G+HVCF
Sbjct: 61  HKDSIRTICYGSNGQLFVSAGDDKTVKIWSTESWRCIYNVGSEKRVSAAAISPNGMHVCF 120

Query: 149 ADKFGVIWVVDLDGGIDENQPLANKTAVSLLSHYCSIITSLKFSLDGRFLVSADRDFKIR 208
           ADKFGVIWVVDLDGG+D NQPL NK AVSLLSHYCSIITSL+FSLDGRFLVSADRDFKIR
Sbjct: 121 ADKFGVIWVVDLDGGVDGNQPLVNKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIR 180

Query: 209 VTTFPTKPVDEAHEIQSFCLGHTDFVSCVAFISNLECPHGFLISGSGDSTVRLWDILSGS 268
           VTTFPTKPV+EAHEIQSFCLGHTDFVSC+AFIS LECPHGFLISGSGDSTVRLWDILSGS
Sbjct: 181 VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISKLECPHGFLISGSGDSTVRLWDILSGS 240

Query: 269 LLDIFEIGDKVELLESKEREGCNSAVTSLCTIADGPLVAVAVQRQIFLFFFSFLHGIILL 328
           LL   E+GDKVELLESKEREGC+SAVTS+CTI DG LVAVA+Q          LHGIILL
Sbjct: 241 LLATCEVGDKVELLESKEREGCHSAVTSICTITDGALVAVAIQS---------LHGIILL 300

Query: 329 NCDLSNRTLSVVKVVSTREETFIPTSVSSSCSTGYLWMVTGVSSLHDVGQSSLACVKVIS 388
           NCDLSN TLSVVKVVST EETFIPTS+SSS ST YLWMVTGVS LH++G +SLA VKVIS
Sbjct: 301 NCDLSNHTLSVVKVVSTSEETFIPTSLSSSFSTRYLWMVTGVSGLHNLGHASLARVKVIS 360

Query: 389 GFNKDNPYSTGNELFVLEDDAIPGGKKLLEKLQGGVSFDEKVFLAAAEAVKTAMSNLLTK 448
           GFNKDNP  T NEL +LEDDAIPGG+KLLEKLQGGV+FDEKVFLAAAEAVKTAMSNLLTK
Sbjct: 361 GFNKDNPNFTENELSILEDDAIPGGEKLLEKLQGGVTFDEKVFLAAAEAVKTAMSNLLTK 420

Query: 449 KQYSTEKRDFRKRTRNDRKLK 469
           KQYSTEKRDFRKRTRNDRKLK
Sbjct: 421 KQYSTEKRDFRKRTRNDRKLK 432

BLAST of MC09g0727 vs. ExPASy TrEMBL
Match: A0A6J1JND1 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit OS=Cucurbita maxima OX=3661 GN=LOC111488451 PE=3 SV=1)

HSP 1 Score: 753 bits (1943), Expect = 6.14e-272
Identity = 385/441 (87.30%), Postives = 404/441 (91.61%), Query Frame = 0

Query: 29  MEETPVEEGDTNKDVEVAPALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLVDESGEQH 88
           MEETPVE GDT KDVE+APALIAVHPAEKSVAVAVGSDLRVFDLV NCPVSL+DESG  +
Sbjct: 1   MEETPVE-GDTKKDVEIAPALIAVHPAEKSVAVAVGSDLRVFDLVDNCPVSLIDESGGSY 60

Query: 89  HKDSIRAICYGSKGQLFVSAGDDKTVKIWSTESWRCIYNVCSEKRVSAAAISPDGLHVCF 148
           HKDSIR ICYGS GQLFVSAGDDKTVKIWSTESWRCIYNV SEKRVSAAAISP+G+HVCF
Sbjct: 61  HKDSIRTICYGSNGQLFVSAGDDKTVKIWSTESWRCIYNVGSEKRVSAAAISPNGMHVCF 120

Query: 149 ADKFGVIWVVDLDGGIDENQPLANKTAVSLLSHYCSIITSLKFSLDGRFLVSADRDFKIR 208
           ADKFGVIWVVDLDGG+D NQPL NK AVSLLSHYCSIITSL+FSLDGRFLVSADRDFKIR
Sbjct: 121 ADKFGVIWVVDLDGGVDGNQPLVNKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIR 180

Query: 209 VTTFPTKPVDEAHEIQSFCLGHTDFVSCVAFISNLECPHGFLISGSGDSTVRLWDILSGS 268
           VTTFPTKPV+EAHEIQSFCLGHTDFVSC+AFIS LECPHGFLISGSGDSTVRLWDILSGS
Sbjct: 181 VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISKLECPHGFLISGSGDSTVRLWDILSGS 240

Query: 269 LLDIFEIGDKVELLESKEREGCNSAVTSLCTIADGPLVAVAVQRQIFLFFFSFLHGIILL 328
           LL   EIGDKVELLESKEREGC+SAVTS+CTIADG LVAVA+Q          LHGIILL
Sbjct: 241 LLATCEIGDKVELLESKEREGCHSAVTSICTIADGTLVAVAIQS---------LHGIILL 300

Query: 329 NCDLSNRTLSVVKVVSTREETFIPTSVSSSCSTGYLWMVTGVSSLHDVGQSSLACVKVIS 388
           NCDLSN +LSVVKVVS  EETFIPTS+SSSCST YLWMVTGVS LH++G SSL  VKVIS
Sbjct: 301 NCDLSNHSLSVVKVVSISEETFIPTSLSSSCSTRYLWMVTGVSGLHNLGHSSLVRVKVIS 360

Query: 389 GFNKDNPYSTGNELFVLEDDAIPGGKKLLEKLQGGVSFDEKVFLAAAEAVKTAMSNLLTK 448
           GFNKDNP ST NEL +LEDDAIPGG+KLLEKLQGGV+FDEKVFLAAAEAVKTAMSNLLTK
Sbjct: 361 GFNKDNPNSTENELSILEDDAIPGGEKLLEKLQGGVTFDEKVFLAAAEAVKTAMSNLLTK 420

Query: 449 KQYSTEKRDFRKRTRNDRKLK 469
           KQYSTEKRDFRKRTRNDRKLK
Sbjct: 421 KQYSTEKRDFRKRTRNDRKLK 431

BLAST of MC09g0727 vs. ExPASy TrEMBL
Match: A0A0A0KAG0 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit OS=Cucumis sativus OX=3659 GN=Csa_7G387800 PE=3 SV=1)

HSP 1 Score: 752 bits (1942), Expect = 8.09e-272
Identity = 383/441 (86.85%), Postives = 406/441 (92.06%), Query Frame = 0

Query: 29  MEETPVEEGDTNKDVEVAPALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLVDESGEQH 88
           MEETPV+EGDTNKDVEVAPALIA+HP+E SVAVAVGSDLRVFDLVGNC VSLVDESG  +
Sbjct: 1   MEETPVDEGDTNKDVEVAPALIALHPSENSVAVAVGSDLRVFDLVGNCAVSLVDESGPPY 60

Query: 89  HKDSIRAICYGSKGQLFVSAGDDKTVKIWSTESWRCIYNVCSEKRVSAAAISPDGLHVCF 148
           HKDSIR ICYGSKGQLF+SAGDDKTVKIWSTESWRCIYNV SEKRVSAAAISP+GLHVCF
Sbjct: 61  HKDSIRTICYGSKGQLFISAGDDKTVKIWSTESWRCIYNVVSEKRVSAAAISPNGLHVCF 120

Query: 149 ADKFGVIWVVDLDGGIDENQPLANKTAVSLLSHYCSIITSLKFSLDGRFLVSADRDFKIR 208
           ADKFGVIWVVDLDGG+D NQPL NK AVSLLSHYCSIITSLKFSLDGRFLVSADRDFKIR
Sbjct: 121 ADKFGVIWVVDLDGGVDGNQPLVNKKAVSLLSHYCSIITSLKFSLDGRFLVSADRDFKIR 180

Query: 209 VTTFPTKPVDEAHEIQSFCLGHTDFVSCVAFISNLECPHGFLISGSGDSTVRLWDILSGS 268
           VT FPTKPV+EAHEIQSFCLGHTDFVSC+AF+SNL+C HGFLISGSGDSTVRLWDILSGS
Sbjct: 181 VTMFPTKPVNEAHEIQSFCLGHTDFVSCIAFVSNLQCSHGFLISGSGDSTVRLWDILSGS 240

Query: 269 LLDIFEIGDKVELLESKEREGCNSAVTSLCTIADGPLVAVAVQRQIFLFFFSFLHGIILL 328
           LLD  EIGDKVELL+SKEREGC+SAVTSLCTIADG LVAVAVQ          LHGIILL
Sbjct: 241 LLDTCEIGDKVELLDSKEREGCHSAVTSLCTIADGALVAVAVQS---------LHGIILL 300

Query: 329 NCDLSNRTLSVVKVVSTREETFIPTSVSSSCSTGYLWMVTGVSSLHDVGQSSLACVKVIS 388
           NCDLSN TLSVVKVVS  EETFIPTS+S+SCSTGYLWMVTGVSSLH++G SSLA VKV+S
Sbjct: 301 NCDLSNHTLSVVKVVSISEETFIPTSLSTSCSTGYLWMVTGVSSLHNLGHSSLAHVKVMS 360

Query: 389 GFNKDNPYSTGNELFVLEDDAIPGGKKLLEKLQGGVSFDEKVFLAAAEAVKTAMSNLLTK 448
           GFNKDN  ST N++ V+EDDAIPGG KLLEKLQGG++FDEKVFLAAAEAVKTAMSNLLTK
Sbjct: 361 GFNKDN--STANDVSVVEDDAIPGGTKLLEKLQGGITFDEKVFLAAAEAVKTAMSNLLTK 420

Query: 449 KQYSTEKRDFRKRTRNDRKLK 469
           KQYS EKRDFRKRTRNDRKLK
Sbjct: 421 KQYSIEKRDFRKRTRNDRKLK 430

BLAST of MC09g0727 vs. ExPASy TrEMBL
Match: A0A5A7VCF2 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold99G00590 PE=3 SV=1)

HSP 1 Score: 748 bits (1930), Expect = 8.52e-270
Identity = 385/444 (86.71%), Postives = 406/444 (91.44%), Query Frame = 0

Query: 29  MEETPVEEGDTNKDVEVAPALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLVDESGEQH 88
           MEETPVEEGDTNKDVEVAPALIA+HP E SVAVAVGSDLRVFDLVGNC V LVDESG  +
Sbjct: 1   MEETPVEEGDTNKDVEVAPALIALHPTENSVAVAVGSDLRVFDLVGNCAVFLVDESGVPY 60

Query: 89  HKDSIRAICYGSKGQLFVSAGDDKTVKIWSTESWRCIYNVCSEKRVSAAAISPDGLHVCF 148
           HKDSIR ICYGSKGQLFVSAGDDKTVKIWSTESWRCIYNV SEKRVSAAAISP+GLHVCF
Sbjct: 61  HKDSIRTICYGSKGQLFVSAGDDKTVKIWSTESWRCIYNVVSEKRVSAAAISPNGLHVCF 120

Query: 149 ADKFGVIWVVDLDGGIDENQPLANKTAVSLLSHYCSIITSLKFSLDGRFLVSADRDFKIR 208
           ADKFGVIWVVDLDGG+D NQPL +K A+SLLSHYCSIITSLKFSLDGRFLVSADRDFKIR
Sbjct: 121 ADKFGVIWVVDLDGGVDGNQPLVSKKAMSLLSHYCSIITSLKFSLDGRFLVSADRDFKIR 180

Query: 209 VTTFPTKPVDEAHEIQSFCLGHTDFVSCVAFISNLECPHGFLISGSGDSTVRLWDILSGS 268
           VTTFPTKPV+EA EIQSFCLGHTDFVSC+AFISNLEC HGFLISGSGDSTVRLWDIL GS
Sbjct: 181 VTTFPTKPVNEAREIQSFCLGHTDFVSCIAFISNLECSHGFLISGSGDSTVRLWDILCGS 240

Query: 269 LLDIFEIGDKVELLESKEREGCNSAVTSLCTIADGPLVAVAVQRQI---FLFFFSFLHGI 328
           LLD  +IGDKVELL+SKEREGC+SAVTSLCTIADG LVAVAVQRQ     LFF   LHGI
Sbjct: 241 LLDTCDIGDKVELLDSKEREGCHSAVTSLCTIADGALVAVAVQRQSSPSMLFFLLCLHGI 300

Query: 329 ILLNCDLSNRTLSVVKVVSTREETFIPTSVSSSCSTGYLWMVTGVSSLHDVGQSSLACVK 388
           ILLNCDLSN TLSVVKVVS  EETFIPTS+S+SCSTGYLWMVTGVSSLH++G SSLA VK
Sbjct: 301 ILLNCDLSNHTLSVVKVVSISEETFIPTSLSTSCSTGYLWMVTGVSSLHNLGHSSLARVK 360

Query: 389 VISGFNKDNPYSTGNELFVLEDDAIPGGKKLLEKLQGGVSFDEKVFLAAAEAVKTAMSNL 448
           V+SGFNKDN   T N++ V+EDDAIPG  KLLEKLQGGV+FD+KVFLAAAEAVKTAMSNL
Sbjct: 361 VMSGFNKDN--YTANDVSVVEDDAIPGETKLLEKLQGGVTFDDKVFLAAAEAVKTAMSNL 420

Query: 449 LTKKQYSTEKRDFRKRTRNDRKLK 469
           LTKKQYS EKRDFRKRTRNDRKLK
Sbjct: 421 LTKKQYSMEKRDFRKRTRNDRKLK 442

BLAST of MC09g0727 vs. TAIR 10
Match: AT1G03110.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 486.1 bits (1250), Expect = 3.2e-137
Identity = 255/439 (58.09%), Postives = 317/439 (72.21%), Query Frame = 0

Query: 30  EETPVEEGDTNKDVEVAPALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLVDESGEQHH 89
           EE+ +EE +    +EVAPALI+VHP++KSVAVAVGSDLR+FDL+ NCPVSLVDES     
Sbjct: 3   EESHIEEVEIQNKLEVAPALISVHPSQKSVAVAVGSDLRIFDLIENCPVSLVDESDGPIR 62

Query: 90  KDSIRAICYGSKGQLFVSAGDDKTVKIWSTESWRCIYNVCSEKRVSAAAISPDGLHVCFA 149
           K+SIRAI Y + G+LFVSAGDDK VKIWS +SWRC+  VCSEKRVSA AIS D  HVC+A
Sbjct: 63  KESIRAIRYSTSGKLFVSAGDDKLVKIWSADSWRCLNTVCSEKRVSAVAISSDDSHVCYA 122

Query: 150 DKFGVIWVVDLDGGIDENQPLANKTAVSLLSHYCSIITSLKFSLDGRFLVSADRDFKIRV 209
           DKFGV+WV++LD GI++ + L +K    LLSHYCSIITSL+FS DGR+++SADRDFKIRV
Sbjct: 123 DKFGVVWVIELD-GINDGKTLPSKKGALLLSHYCSIITSLEFSPDGRYILSADRDFKIRV 182

Query: 210 TTFPTKPVDEAHEIQSFCLGHTDFVSCVAFISNLECPHGFLISGSGDSTVRLWDILSGSL 269
           T FP KP++ AHEIQSFCLGH++F++C AF+S  E   G+L+SGSGDSTVRLWDI SGSL
Sbjct: 183 TVFPKKPLEGAHEIQSFCLGHSEFITCTAFVSTPELTQGYLMSGSGDSTVRLWDITSGSL 242

Query: 270 LDIFEIGDKVELLESKEREG-CNSAVTSLCTIADGPLVAVAVQRQIFLFFFSFLHGIILL 329
           LD  E+       ES E E      VT +C I +  L AVA+Q            GI LL
Sbjct: 243 LDTCEVSTVAGHAESNENESPTQVTVTDICAIPNSSLAAVAIQS---------FQGIFLL 302

Query: 330 NCDLSNRTLSVVKVVSTREETFIPTSVSSSCSTGYLWMVTGVSSLHDVGQSSLACVKVIS 389
           +CDL+  TLS+ KV+    E+FIPTS++ S S+  LWMV+G S+L        + V+VIS
Sbjct: 303 SCDLTAHTLSITKVIKIPGESFIPTSIAVSASSRLLWMVSGASNLPGSNHPGFSRVRVIS 362

Query: 390 GFNKDNPYSTGNELFVLEDDAIPGGKKLLEKLQGGVSFDEKVFLAAAEAVKTAMSNLLTK 449
               ++         +LED+ IPGG KLLE+LQG V+ +E V  AAAEAV+ AMS+LL K
Sbjct: 363 CLETESS-------SILEDEQIPGGTKLLEQLQGKVTIEESVMSAAAEAVRAAMSSLLMK 422

Query: 450 KQYSTEKRDFRKRTRNDRK 468
           KQYS EKR+FRKRTRND+K
Sbjct: 423 KQYSEEKREFRKRTRNDKK 424

BLAST of MC09g0727 vs. TAIR 10
Match: AT5G50120.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 72.4 bits (176), Expect = 1.1e-12
Identity = 54/182 (29.67%), Postives = 90/182 (49.45%), Query Frame = 0

Query: 88  HHKDSIRAICYGSKGQLFVSAGDDKTVKIWSTESWRCI--YNVCSEKRVSAAAISPDG-L 147
           HH D++  +     G L  S   D+T+KIW T  ++C+  +    +  ++A A+S +G +
Sbjct: 163 HHVDAVSGLALSRDGTLLYSVSWDRTLKIWRTTDFKCLESFTNAHDDAINAVALSENGDI 222

Query: 148 HVCFADKFGVIWVVDLDGGIDENQPLANK-TAVSLLSHYCSIITSLKFS-LDGRFLVSAD 207
           +   +D+   +W  +++   +EN     K + V++LS + S I +L  S  +G  L S  
Sbjct: 223 YTGSSDQRIKVWRKNIN---EENVKKKRKHSLVAILSEHNSGINALALSGTNGSLLHSGG 282

Query: 208 RDFKIRVTTFPTKPVDEAHEI--QSFCLGHTDFVSCVAFISNLECPHGFLISGSGDSTVR 263
            D  I V        D+  +I       GHT+ V C+A +S++ C      SGS D TVR
Sbjct: 283 SDGSILVW-----ERDDGGDIVVVGMLRGHTESVLCLAVVSDILC------SGSADKTVR 330

BLAST of MC09g0727 vs. TAIR 10
Match: AT1G24130.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 71.2 bits (173), Expect = 2.4e-12
Identity = 48/180 (26.67%), Postives = 83/180 (46.11%), Query Frame = 0

Query: 88  HHKDSIRAICYGSKGQLFVSAGDDKTVKIWSTESWRCIYNV--CSEKRVSAAAISPDG-L 147
           HH D++ ++     G L  SA  D++ KIW T  ++C+ ++    +  ++A  +S DG +
Sbjct: 190 HHVDAVSSLALSQDGSLLYSASWDRSFKIWRTSDFKCLDSIEKAHDDAINAIVVSKDGFV 249

Query: 148 HVCFADKFGVIWVVDLDGGIDENQPLANKTAVSLLSHYCSIITSLKFSLDGRFLVSADRD 207
           +   ADK   +W          N+     + V+ L+ + S + +L  S DG+ L S   D
Sbjct: 250 YTGSADKKIKVW----------NKKDKKHSLVATLTKHLSAVNALAISEDGKVLYSGACD 309

Query: 208 FKIRVTTFPTKPVDEA--HEIQSFCLGHTDFVSCVAFISNLECPHGFLISGSGDSTVRLW 263
             I V        DE     +     GH   + C+A  S+L      ++SGS D ++R+W
Sbjct: 310 RSILVWERLINGDDEELHMSVVGALRGHRKAIMCLAVASDL------VLSGSADKSLRVW 353

BLAST of MC09g0727 vs. TAIR 10
Match: AT3G49660.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 63.2 bits (152), Expect = 6.6e-10
Identity = 37/92 (40.22%), Postives = 53/92 (57.61%), Query Frame = 0

Query: 179 LSHYCSIITSLKFSLDGRFLVSADRDFKIRVTTFPTKPVDEAHEIQSFCLGHTDFVSCVA 238
           L+ +   ++S+KFS DGR L SA  D  IR  T  T     A  +Q F  GH + +S VA
Sbjct: 20  LTSHNRAVSSVKFSSDGRLLASASADKTIRTYTINTINDPIAEPVQEF-TGHENGISDVA 79

Query: 239 FISNLECPHGFLISGSGDSTVRLWDILSGSLL 271
           F S+      F++S S D T++LWD+ +GSL+
Sbjct: 80  FSSDAR----FIVSASDDKTLKLWDVETGSLI 106

BLAST of MC09g0727 vs. TAIR 10
Match: AT1G18080.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 59.7 bits (143), Expect = 7.3e-09
Identity = 53/186 (28.49%), Postives = 79/186 (42.47%), Query Frame = 0

Query: 89  HKDSIRAICYG-SKGQLFVSAGDDKTVKIWSTESWRCIYNVCSEKR------VSAAAISP 148
           H D + AI        + VSA  DK++ +W        Y V   +       V    +S 
Sbjct: 14  HTDMVTAIATPIDNADIIVSASRDKSIILWKLTKDDKAYGVAQRRLTGHSHFVEDVVLSS 73

Query: 149 DGLHVCFADKFGVIWVVDLDGGIDENQPLANKTAVSLLSHYCSIITSLKFSLDGRFLVSA 208
           DG         G + + DL  G+   +          + H   ++ S+ FSLD R +VSA
Sbjct: 74  DGQFALSGSWDGELRLWDLAAGVSTRR---------FVGHTKDVL-SVAFSLDNRQIVSA 133

Query: 209 DRDFKIRV--TTFPTK-PVDEAHEIQSFCLGHTDFVSCVAFISNLECPHGFLISGSGDST 265
            RD  I++  T    K  + E  E      GH D+VSCV F  N   P   ++S S D T
Sbjct: 134 SRDRTIKLWNTLGECKYTISEGGE------GHRDWVSCVRFSPNTLQP--TIVSASWDKT 181

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q54UI36.6e-2331.13tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 OS=Dictyosteli... [more]
Q7ZY781.3e-2128.47tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 OS=Xenopus lae... [more]
Q5FVB61.3e-1829.36tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 OS=Xenopus tro... [more]
A4IGH45.4e-1731.76tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 OS=Danio rerio... [more]
P570819.3e-1730.73tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4 OS=Homo sapien... [more]
Match NameE-valueIdentityDescription
XP_022150294.11.04e-30997.74tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 [Momordica cha... [more]
XP_023549061.13.56e-27687.98tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 [Cucurbita pep... [more]
KAG7014455.11.35e-27485.59F-box/kelch-repeat protein SKIP30 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022954128.11.68e-27487.53tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 [Cucurbita mos... [more]
XP_022991957.11.27e-27187.30tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 [Cucurbita max... [more]
Match NameE-valueIdentityDescription
A0A6J1D9K95.05e-31097.74tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit OS=Momordica charan... [more]
A0A6J1GQ898.15e-27587.53tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit OS=Cucurbita moscha... [more]
A0A6J1JND16.14e-27287.30tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit OS=Cucurbita maxima... [more]
A0A0A0KAG08.09e-27286.85tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit OS=Cucumis sativus ... [more]
A0A5A7VCF28.52e-27086.71tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit OS=Cucumis melo var... [more]
Match NameE-valueIdentityDescription
AT1G03110.13.2e-13758.09Transducin/WD40 repeat-like superfamily protein [more]
AT5G50120.11.1e-1229.67Transducin/WD40 repeat-like superfamily protein [more]
AT1G24130.12.4e-1226.67Transducin/WD40 repeat-like superfamily protein [more]
AT3G49660.16.6e-1040.22Transducin/WD40 repeat-like superfamily protein [more]
AT1G18080.17.3e-0928.49Transducin/WD40 repeat-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001680WD40 repeatSMARTSM00320WD40_4coord: 121..159
e-value: 150.0
score: 3.2
coord: 219..263
e-value: 5.9E-5
score: 32.5
coord: 80..118
e-value: 1.5E-4
score: 31.1
coord: 172..211
e-value: 0.32
score: 20.1
IPR001680WD40 repeatPFAMPF00400WD40coord: 180..209
e-value: 0.012
score: 16.5
coord: 227..263
e-value: 4.7E-4
score: 20.9
coord: 88..118
e-value: 1.3E-4
score: 22.7
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 227..272
score: 12.246197
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 86..127
score: 12.881143
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3D2.130.10.10coord: 47..128
e-value: 1.2E-12
score: 49.7
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3D2.130.10.10coord: 129..316
e-value: 5.5E-24
score: 87.2
IPR028884tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunitPANTHERPTHR16288WD40 REPEAT PROTEIN 4coord: 48..467
IPR028884tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunitHAMAPMF_03056TRM82coord: 56..462
score: 22.814522
IPR019775WD40 repeat, conserved sitePROSITEPS00678WD_REPEATS_1coord: 250..264
NoneNo IPR availablePROSITEPS50294WD_REPEATS_REGIONcoord: 86..121
score: 10.363778
NoneNo IPR availablePROSITEPS50294WD_REPEATS_REGIONcoord: 227..264
score: 9.678328
IPR036322WD40-repeat-containing domain superfamilySUPERFAMILY50978WD40 repeat-likecoord: 50..317

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g0727.1MC09g0727.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0036265 RNA (guanine-N7)-methylation
biological_process GO:0106004 tRNA (guanine-N7)-methylation
cellular_component GO:0005829 cytosol
cellular_component GO:0005634 nucleus
cellular_component GO:0043527 tRNA methyltransferase complex
molecular_function GO:0005515 protein binding
molecular_function GO:0008176 tRNA (guanine-N7-)-methyltransferase activity