MC09g0697 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g0697
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionAdenylate isopentenyltransferase
LocationMC09: 6460241 .. 6461044 (-)
RNA-Seq ExpressionMC09g0697
SyntenyMC09g0697
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAAAGAGAAGGTGGTGATTGTGATCGGCGCCACCGGCACGGGCAAGTCCCGACTATCGATCGACATCGCCACCCGGTTCTCATCGGAAGTCATCAACTCCGACAAAATGCAAGTCTACGAGGGTCTCGACGTAGTAACCAACAAGGTCACCAAGCAAGAACAACAGGGAGTGCCCCATCACTTGCTCGGAACAATCAATCCCTTCTATGATTTCTCGGCGACCGACTTCTCCGACATGGCCGGGCTTTCCTTGGACGCTATCGCCGCCCGCGATCGCCTCCCGATCATCGCTGGGGGCTCTAATTCTTACATAGAAGCCTTAATCGACCCAAACTTTCAATCGAGATACGAATGTTGTTTCCTTTGGGTGGATGTGTCAATGCCTGTATTGTGTTCCTTTGTATCCAAACGAGTAGACAAGATGGTAGCGAACGGAATGGTCGACGAGGCTGAAAAGTTCTTTGATCCTGCCAGAGATTATTCTCGTGGGATCAAAAGGGCAATCGGGGTTCCTGAATTCGACTCGTATTTTCGATACAAATCATTGCTAGACGAAGAAACTCAAGATAAACTTCTCCAAGATGCAATATCAAAAGTGAAGGACCACACATGCAGGCTAGCATTGAGACAATTAGGGAAAATCCAACGGCTCAAAAACGTCAAGGGATGGAAGATACACCAATTGGATGCAACAGAGGCCTTTACAAAGCAAGGGAAAGAATCAGAGGAAGCATGGAACAAGCTTGTGGCGGCACCGAGCATGGCGATAATCGAAAAATTTCTTCACCAAAACGTCTTCACG

mRNA sequence

CAAAAAGAGAAGGTGGTGATTGTGATCGGCGCCACCGGCACGGGCAAGTCCCGACTATCGATCGACATCGCCACCCGGTTCTCATCGGAAGTCATCAACTCCGACAAAATGCAAGTCTACGAGGGTCTCGACGTAGTAACCAACAAGGTCACCAAGCAAGAACAACAGGGAGTGCCCCATCACTTGCTCGGAACAATCAATCCCTTCTATGATTTCTCGGCGACCGACTTCTCCGACATGGCCGGGCTTTCCTTGGACGCTATCGCCGCCCGCGATCGCCTCCCGATCATCGCTGGGGGCTCTAATTCTTACATAGAAGCCTTAATCGACCCAAACTTTCAATCGAGATACGAATGTTGTTTCCTTTGGGTGGATGTGTCAATGCCTGTATTGTGTTCCTTTGTATCCAAACGAGTAGACAAGATGGTAGCGAACGGAATGGTCGACGAGGCTGAAAAGTTCTTTGATCCTGCCAGAGATTATTCTCGTGGGATCAAAAGGGCAATCGGGGTTCCTGAATTCGACTCGTATTTTCGATACAAATCATTGCTAGACGAAGAAACTCAAGATAAACTTCTCCAAGATGCAATATCAAAAGTGAAGGACCACACATGCAGGCTAGCATTGAGACAATTAGGGAAAATCCAACGGCTCAAAAACGTCAAGGGATGGAAGATACACCAATTGGATGCAACAGAGGCCTTTACAAAGCAAGGGAAAGAATCAGAGGAAGCATGGAACAAGCTTGTGGCGGCACCGAGCATGGCGATAATCGAAAAATTTCTTCACCAAAACGTCTTCACG

Coding sequence (CDS)

CAAAAAGAGAAGGTGGTGATTGTGATCGGCGCCACCGGCACGGGCAAGTCCCGACTATCGATCGACATCGCCACCCGGTTCTCATCGGAAGTCATCAACTCCGACAAAATGCAAGTCTACGAGGGTCTCGACGTAGTAACCAACAAGGTCACCAAGCAAGAACAACAGGGAGTGCCCCATCACTTGCTCGGAACAATCAATCCCTTCTATGATTTCTCGGCGACCGACTTCTCCGACATGGCCGGGCTTTCCTTGGACGCTATCGCCGCCCGCGATCGCCTCCCGATCATCGCTGGGGGCTCTAATTCTTACATAGAAGCCTTAATCGACCCAAACTTTCAATCGAGATACGAATGTTGTTTCCTTTGGGTGGATGTGTCAATGCCTGTATTGTGTTCCTTTGTATCCAAACGAGTAGACAAGATGGTAGCGAACGGAATGGTCGACGAGGCTGAAAAGTTCTTTGATCCTGCCAGAGATTATTCTCGTGGGATCAAAAGGGCAATCGGGGTTCCTGAATTCGACTCGTATTTTCGATACAAATCATTGCTAGACGAAGAAACTCAAGATAAACTTCTCCAAGATGCAATATCAAAAGTGAAGGACCACACATGCAGGCTAGCATTGAGACAATTAGGGAAAATCCAACGGCTCAAAAACGTCAAGGGATGGAAGATACACCAATTGGATGCAACAGAGGCCTTTACAAAGCAAGGGAAAGAATCAGAGGAAGCATGGAACAAGCTTGTGGCGGCACCGAGCATGGCGATAATCGAAAAATTTCTTCACCAAAACGTCTTCACG

Protein sequence

QKEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPHHLLGTINPFYDFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECCFLWVDVSMPVLCSFVSKRVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDKLLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGKESEEAWNKLVAAPSMAIIEKFLHQNVFT
Homology
BLAST of MC09g0697 vs. ExPASy Swiss-Prot
Match: Q94ID2 (Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT5 PE=1 SV=2)

HSP 1 Score: 311.6 bits (797), Expect = 8.6e-84
Identity = 159/267 (59.55%), Postives = 206/267 (77.15%), Query Frame = 0

Query: 1   QKEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPH 60
           +K+KVV V+GATGTGKSRL+ID+ATRF +E++NSDK+QVY+GLD+VTNKVT +E  GVPH
Sbjct: 31  RKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDIVTNKVTPEESLGVPH 90

Query: 61  HLLGTINPFY-DFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDP--NFQSRY 120
           HLLGT++  Y DF+A DF   A  ++++I  RDR+PIIAGGSNSYIEAL++   +F+ RY
Sbjct: 91  HLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAGGSNSYIEALVNDCVDFRLRY 150

Query: 121 ECCFLWVDVSMPVLCSFVSKRVDKMVANGMVDEAEKFFDP-ARDYSRGIKRAIGVPEFDS 180
            CCFLWVDVS PVL SFVS+RVDKMV  G+VDE  + FDP + DYS GI+RAIGVPE D 
Sbjct: 151 NCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDPSSSDYSAGIRRAIGVPELDE 210

Query: 181 YFRYKSL-LDEETQDKLLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWKIHQLDATEAF 240
           + R +      ET ++LL+ AI K+K++TC LA RQL KIQRL     W +H++DATE F
Sbjct: 211 FLRSEMRNYPAETTERLLETAIEKIKENTCLLACRQLQKIQRLYKQWKWNMHRVDATEVF 270

Query: 241 TKQGKESEEAWNKLVAAPSMAIIEKFL 263
            ++G+E++EAW+  VA PS   +EKFL
Sbjct: 271 LRRGEEADEAWDNSVAHPSALAVEKFL 297

BLAST of MC09g0697 vs. ExPASy Swiss-Prot
Match: Q93WC9 (Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT3 PE=1 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 5.2e-81
Identity = 148/267 (55.43%), Postives = 202/267 (75.66%), Query Frame = 0

Query: 2   KEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPHH 61
           K+KVV+++GATGTGKSRLS+DIATRF +E+INSDK+QV++GLD+VTNK+T +E  GVPHH
Sbjct: 40  KDKVVVIMGATGTGKSRLSVDIATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHH 99

Query: 62  LLGTINPFYDFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDP---NFQSRYE 121
           LLG + P  D +A ++  MA LS++++  R +LPII GGSNSY+EAL+D     F+SRY+
Sbjct: 100 LLGVLPPEADLTAANYCHMANLSIESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYD 159

Query: 122 CCFLWVDVSMPVLCSFVSKRVDKMVANGMVDEAEKFFDPAR-DYSRGIKRAIGVPEFDSY 181
           CCFLWVDV++PVL  FVS+RVDKMV +GMV+E  +FFD +  DYSRGIK+AIG PEFD +
Sbjct: 160 CCFLWVDVALPVLHGFVSERVDKMVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRF 219

Query: 182 FRYKSLLDEETQDKLLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTK 241
           FR +  L+ E +++LL   + ++K +T  LA RQ  KI+RL+ VK W I ++DAT  FTK
Sbjct: 220 FRNEQFLNVEDREELLSKVLEEIKRNTFELACRQREKIERLRKVKKWSIQRVDATPVFTK 279

Query: 242 QGK--ESEEAWNKLVAAPSMAIIEKFL 263
           +    ++  AW +LVA PS   + +FL
Sbjct: 280 RRSKMDANVAWERLVAGPSTDTVSRFL 306

BLAST of MC09g0697 vs. ExPASy Swiss-Prot
Match: Q94ID1 (Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IPT7 PE=2 SV=2)

HSP 1 Score: 281.2 bits (718), Expect = 1.2e-74
Identity = 145/271 (53.51%), Postives = 201/271 (74.17%), Query Frame = 0

Query: 2   KEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPHH 61
           KEKV+ V+GATG+GKSRL+ID+ATRF  E+INSDK+Q+Y+GLDV+TNKVT +E +GVPHH
Sbjct: 33  KEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLTNKVTPKECRGVPHH 92

Query: 62  LLGTI-NPFYDFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDPN---FQSRY 121
           LLG   +   + +AT +S +A  ++  ++A ++LPI+AGGSNSYIEAL++ +     + Y
Sbjct: 93  LLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIEALVNHSSGFLLNNY 152

Query: 122 ECCFLWVDVSMPVLCSFVSKRVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSY 181
           +CCF+WVDVS+PVL SFVSKRVD+M+  G+++E  + F+P  +YS GI+RAIGVPE   Y
Sbjct: 153 DCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNPKANYSVGIRRAIGVPELHEY 212

Query: 182 FRYKSLLDEETQDKLLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWK--IHQLDATEAF 241
            R +SL+D  T+ K+L  A+  +K +T  LA RQL KIQRL   K WK  +H++DATE F
Sbjct: 213 LRNESLVDRATKSKMLDVAVKNIKKNTEILACRQLKKIQRLH--KKWKMSMHRVDATEVF 272

Query: 242 TKQG-KESEEAWNKLVAAPSMAIIEKFLHQN 266
            K+  +E +EAW  LVA PS  I++KF + N
Sbjct: 273 LKRNVEEQDEAWENLVARPSERIVDKFYNNN 301

BLAST of MC09g0697 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 1.3e-63
Identity = 133/303 (43.89%), Postives = 188/303 (62.05%), Query Frame = 0

Query: 1   QKEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPH 60
           +KEK+++++GATGTGKSRLSID+A  F  EVINSDKMQVY+GLD+ TNK++  ++ GVPH
Sbjct: 28  RKEKLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLDITTNKISVPDRGGVPH 87

Query: 61  HLLGTINPFY-DFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDPNFQS---- 120
           HLLG ++P   + +  DF  +AG ++  I  R +LP++ GGSNS+I AL+   F S    
Sbjct: 88  HLLGEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNSFIHALLVDRFDSSGPG 147

Query: 121 --------------RYECCFLWVDVSMPVLCSFVSKRVDKMVANGMVDEAEKFFDPA-RD 180
                         RY+CCFLWVDVS+ VL  +++KRVD M+  GM DE  +F+ P   D
Sbjct: 148 VFEEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLELGMFDELAEFYSPEDED 207

Query: 181 YSR------GIKRAIGVPEFDSYFRYKSLLDEETQDK--------LLQDAISKVKDHTCR 240
           +        G+++AIGVPEFD YF      D E +D           ++A+  +K++TC 
Sbjct: 208 HDEDSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGRDRVRRGAFEEAVRAIKENTCH 267

Query: 241 LALRQLGKIQRLKNVKGWKIHQLDATEAF-----TKQGKESEEAWNKLVAAPSMAIIEKF 265
           LA RQ+GKI RLK   GW + +LDATE+F     +  G++  E W K V  PS+ I+ +F
Sbjct: 268 LAKRQIGKILRLKGA-GWDLRRLDATESFRAAMTSDSGEKCTEIWEKQVLEPSVKIVSRF 327

BLAST of MC09g0697 vs. ExPASy Swiss-Prot
Match: Q94ID3 (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT1 PE=1 SV=2)

HSP 1 Score: 242.3 bits (617), Expect = 6.4e-63
Identity = 132/296 (44.59%), Postives = 194/296 (65.54%), Query Frame = 0

Query: 1   QKEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPH 60
           +K+KVV+++GATG GKSRLS+D+ATRF SE+INSDK+QVYEGL++ TN++T Q+++GVPH
Sbjct: 63  RKDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPH 122

Query: 61  HLLGTINPFY-DFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALI----DPNFQS 120
           HLLG INP + + +A +F   A   +  I +R ++PIIAGGSNS++ AL+    DP F  
Sbjct: 123 HLLGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDP 182

Query: 121 ------------RYECCFLWVDVSMPVLCSFVSKRVDKMVANGMVDEAEKFFDPAR---D 180
                       RYECCF+WVDVS  VL  ++ +RVD+M+ +GM +E  +F+DP +   +
Sbjct: 183 FSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLE 242

Query: 181 YSRGIKRAIGVPEFDSYFR----YKSLLDEETQDKLLQD-AISKVKDHTCRLALRQLGKI 240
              GI++AIGVPEFD YF+     K ++  +   K   D A+  +K +T  LA RQ+ KI
Sbjct: 243 TRFGIRKAIGVPEFDGYFKEYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKI 302

Query: 241 QRLKNVKGWKIHQLDATEAF------TKQGKESEEAWNKLVAAPSMAIIEKFLHQN 266
           + LK+  GW+I ++DAT +F      +   K+  E W + V  PS+ I+++ L QN
Sbjct: 303 EMLKDA-GWEIERVDATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIVKRHLVQN 357

BLAST of MC09g0697 vs. NCBI nr
Match: XP_022150248.1 (adenylate isopentenyltransferase 3, chloroplastic [Momordica charantia])

HSP 1 Score: 536 bits (1381), Expect = 3.71e-191
Identity = 268/268 (100.00%), Postives = 268/268 (100.00%), Query Frame = 0

Query: 1   QKEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPH 60
           QKEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPH
Sbjct: 38  QKEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPH 97

Query: 61  HLLGTINPFYDFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECC 120
           HLLGTINPFYDFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECC
Sbjct: 98  HLLGTINPFYDFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECC 157

Query: 121 FLWVDVSMPVLCSFVSKRVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRY 180
           FLWVDVSMPVLCSFVSKRVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRY
Sbjct: 158 FLWVDVSMPVLCSFVSKRVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRY 217

Query: 181 KSLLDEETQDKLLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGK 240
           KSLLDEETQDKLLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGK
Sbjct: 218 KSLLDEETQDKLLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGK 277

Query: 241 ESEEAWNKLVAAPSMAIIEKFLHQNVFT 268
           ESEEAWNKLVAAPSMAIIEKFLHQNVFT
Sbjct: 278 ESEEAWNKLVAAPSMAIIEKFLHQNVFT 305

BLAST of MC09g0697 vs. NCBI nr
Match: XP_038899604.1 (adenylate isopentenyltransferase 3, chloroplastic [Benincasa hispida])

HSP 1 Score: 461 bits (1186), Expect = 1.80e-161
Identity = 225/266 (84.59%), Postives = 246/266 (92.48%), Query Frame = 0

Query: 1   QKEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPH 60
           QKEKVVIVIGATGTGKSRLS+DIATRF SEVINSDKMQVY GLD+VTNK+TKQEQ+GVPH
Sbjct: 37  QKEKVVIVIGATGTGKSRLSVDIATRFPSEVINSDKMQVYRGLDIVTNKITKQEQRGVPH 96

Query: 61  HLLGTINPFYDFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECC 120
           HLLG INPF DFSAT+FSDMA LSLDAI + DRLPIIAGGSNSYIEALID  F+S+YECC
Sbjct: 97  HLLGIINPFSDFSATNFSDMAMLSLDAITSHDRLPIIAGGSNSYIEALIDSKFRSKYECC 156

Query: 121 FLWVDVSMPVLCSFVSKRVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRY 180
           FLWVDVSM VL SFVS+RVDKMVANGMVDEAEKFFDP RDYSRGIKRAIGVPEFDSYFRY
Sbjct: 157 FLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPTRDYSRGIKRAIGVPEFDSYFRY 216

Query: 181 KSLLDEETQDKLLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGK 240
           K LLDEE QDKLL+++ISKVK+HTC+LALRQLGKIQRL+N+KGW IH+LDATEAFTKQGK
Sbjct: 217 KPLLDEEAQDKLLKESISKVKEHTCKLALRQLGKIQRLRNIKGWNIHRLDATEAFTKQGK 276

Query: 241 ESEEAWNKLVAAPSMAIIEKFLHQNV 266
           E+EEAWNKLV  PS  +I++FLH NV
Sbjct: 277 EAEEAWNKLVTVPSTTLIDQFLHPNV 302

BLAST of MC09g0697 vs. NCBI nr
Match: KAG7025533.1 (Adenylate isopentenyltransferase 3, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 461 bits (1185), Expect = 2.56e-161
Identity = 225/268 (83.96%), Postives = 248/268 (92.54%), Query Frame = 0

Query: 1   QKEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPH 60
           +KEKVVIVIGATGTGKSRLSIDIATRF SEVINSDKMQVY+GLD++TNKVTKQEQ+GVPH
Sbjct: 37  EKEKVVIVIGATGTGKSRLSIDIATRFPSEVINSDKMQVYDGLDIITNKVTKQEQRGVPH 96

Query: 61  HLLGTINPFYDFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECC 120
           HLLG I+PF DFSAT+FSDMA LSLD I  RDRLPIIAGGSNSY+EALID NFQS+YECC
Sbjct: 97  HLLGIIDPFSDFSATNFSDMAMLSLDTIFGRDRLPIIAGGSNSYVEALIDSNFQSKYECC 156

Query: 121 FLWVDVSMPVLCSFVSKRVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRY 180
           FLWVDVSMPVL SFVSKRVDKMVANGMVDEAE+FFDPA DYSRGIKRAIGVPEFDSYFRY
Sbjct: 157 FLWVDVSMPVLHSFVSKRVDKMVANGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRY 216

Query: 181 KSLLDEETQDKLLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGK 240
           +SLLD+ETQ+KLLQ+AISKVK +TC+LA RQ+GKIQRL+N+KGWKIH+LDATEAF KQGK
Sbjct: 217 RSLLDDETQEKLLQEAISKVKKYTCKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGK 276

Query: 241 ESEEAWNKLVAAPSMAIIEKFLHQNVFT 268
           E+EE WNKLV  PS  II++FLH NV T
Sbjct: 277 EAEEEWNKLVTVPSTTIIDQFLHSNVIT 304

BLAST of MC09g0697 vs. NCBI nr
Match: XP_023514910.1 (adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 459 bits (1181), Expect = 1.04e-160
Identity = 224/268 (83.58%), Postives = 248/268 (92.54%), Query Frame = 0

Query: 1   QKEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPH 60
           +KEKVVIVIGATGTGKSRLSIDIATRF SEVINSDKMQVY+GLD++TNKVTKQEQ+GVPH
Sbjct: 37  EKEKVVIVIGATGTGKSRLSIDIATRFPSEVINSDKMQVYDGLDIITNKVTKQEQRGVPH 96

Query: 61  HLLGTINPFYDFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECC 120
           HLLG I+PF DFSAT+FSDMA LSLD I  RDRLPIIAGGSNSY+EALID NFQS+YECC
Sbjct: 97  HLLGIIDPFSDFSATNFSDMAMLSLDTILGRDRLPIIAGGSNSYVEALIDSNFQSKYECC 156

Query: 121 FLWVDVSMPVLCSFVSKRVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRY 180
           FLWVDVSMPVL SFVSKRVDKMVANGMVDEAE+FFDPA DYSRGIKRAIGVPEFDSYFRY
Sbjct: 157 FLWVDVSMPVLHSFVSKRVDKMVANGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRY 216

Query: 181 KSLLDEETQDKLLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGK 240
           +SLL++ETQ+KLLQ+AISKVK +TC+LA RQ+GKIQRL+N+KGWKIH+LDATEAF KQGK
Sbjct: 217 RSLLNDETQEKLLQEAISKVKKYTCKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGK 276

Query: 241 ESEEAWNKLVAAPSMAIIEKFLHQNVFT 268
           E+EE WNKLV  PS  II++FLH NV T
Sbjct: 277 EAEEEWNKLVTVPSTTIIDQFLHSNVIT 304

BLAST of MC09g0697 vs. NCBI nr
Match: XP_022960175.1 (adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 458 bits (1178), Expect = 2.97e-160
Identity = 225/268 (83.96%), Postives = 247/268 (92.16%), Query Frame = 0

Query: 1   QKEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPH 60
           +KEKVVIVIGATGTGKSRLSIDIATRF SEVINSDKMQVY+GLD+VTNKVTKQEQ+GVPH
Sbjct: 37  EKEKVVIVIGATGTGKSRLSIDIATRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPH 96

Query: 61  HLLGTINPFYDFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECC 120
           HLLG I+PF DFSAT+FSDMA LSL  I  RDRLPIIAGGSNSY+EALID NFQS+YECC
Sbjct: 97  HLLGIIDPFSDFSATNFSDMAMLSLVTILGRDRLPIIAGGSNSYVEALIDSNFQSKYECC 156

Query: 121 FLWVDVSMPVLCSFVSKRVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRY 180
           FLWVDVSMPVL SFVSKRVDKMVANGMVDEAE+FFDPA DYSRGIKRAIGVPEFDSYFRY
Sbjct: 157 FLWVDVSMPVLHSFVSKRVDKMVANGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRY 216

Query: 181 KSLLDEETQDKLLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGK 240
           +SLLD+ETQ+KLLQ+AISKVK +TC+LA RQ+GKIQRL+N+KGWKIH+LDATEAF KQGK
Sbjct: 217 RSLLDDETQEKLLQEAISKVKKYTCKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGK 276

Query: 241 ESEEAWNKLVAAPSMAIIEKFLHQNVFT 268
           E+EE WNKLV  PS  II++FLH NV T
Sbjct: 277 EAEEEWNKLVTVPSTTIIDQFLHSNVIT 304

BLAST of MC09g0697 vs. ExPASy TrEMBL
Match: A0A6J1D8Y2 (adenylate isopentenyltransferase 3, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111018461 PE=4 SV=1)

HSP 1 Score: 536 bits (1381), Expect = 1.80e-191
Identity = 268/268 (100.00%), Postives = 268/268 (100.00%), Query Frame = 0

Query: 1   QKEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPH 60
           QKEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPH
Sbjct: 38  QKEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPH 97

Query: 61  HLLGTINPFYDFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECC 120
           HLLGTINPFYDFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECC
Sbjct: 98  HLLGTINPFYDFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECC 157

Query: 121 FLWVDVSMPVLCSFVSKRVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRY 180
           FLWVDVSMPVLCSFVSKRVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRY
Sbjct: 158 FLWVDVSMPVLCSFVSKRVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRY 217

Query: 181 KSLLDEETQDKLLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGK 240
           KSLLDEETQDKLLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGK
Sbjct: 218 KSLLDEETQDKLLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGK 277

Query: 241 ESEEAWNKLVAAPSMAIIEKFLHQNVFT 268
           ESEEAWNKLVAAPSMAIIEKFLHQNVFT
Sbjct: 278 ESEEAWNKLVAAPSMAIIEKFLHQNVFT 305

BLAST of MC09g0697 vs. ExPASy TrEMBL
Match: A0A6J1H8C9 (adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111460993 PE=4 SV=1)

HSP 1 Score: 458 bits (1178), Expect = 1.44e-160
Identity = 225/268 (83.96%), Postives = 247/268 (92.16%), Query Frame = 0

Query: 1   QKEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPH 60
           +KEKVVIVIGATGTGKSRLSIDIATRF SEVINSDKMQVY+GLD+VTNKVTKQEQ+GVPH
Sbjct: 37  EKEKVVIVIGATGTGKSRLSIDIATRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPH 96

Query: 61  HLLGTINPFYDFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECC 120
           HLLG I+PF DFSAT+FSDMA LSL  I  RDRLPIIAGGSNSY+EALID NFQS+YECC
Sbjct: 97  HLLGIIDPFSDFSATNFSDMAMLSLVTILGRDRLPIIAGGSNSYVEALIDSNFQSKYECC 156

Query: 121 FLWVDVSMPVLCSFVSKRVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRY 180
           FLWVDVSMPVL SFVSKRVDKMVANGMVDEAE+FFDPA DYSRGIKRAIGVPEFDSYFRY
Sbjct: 157 FLWVDVSMPVLHSFVSKRVDKMVANGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRY 216

Query: 181 KSLLDEETQDKLLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGK 240
           +SLLD+ETQ+KLLQ+AISKVK +TC+LA RQ+GKIQRL+N+KGWKIH+LDATEAF KQGK
Sbjct: 217 RSLLDDETQEKLLQEAISKVKKYTCKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGK 276

Query: 241 ESEEAWNKLVAAPSMAIIEKFLHQNVFT 268
           E+EE WNKLV  PS  II++FLH NV T
Sbjct: 277 EAEEEWNKLVTVPSTTIIDQFLHSNVIT 304

BLAST of MC09g0697 vs. ExPASy TrEMBL
Match: A0A6J1KSJ2 (adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111497849 PE=4 SV=1)

HSP 1 Score: 457 bits (1177), Expect = 2.04e-160
Identity = 224/268 (83.58%), Postives = 248/268 (92.54%), Query Frame = 0

Query: 1   QKEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPH 60
           +KEKVVIVIGATGTGKSRLSIDIAT+F SEVINSDKMQVY+GLD+VTNKVTKQEQ+GVPH
Sbjct: 37  EKEKVVIVIGATGTGKSRLSIDIATQFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPH 96

Query: 61  HLLGTINPFYDFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECC 120
           HLLG I+PF DFSAT+FSDMA LSLD I  RDRLPIIAGGSNSYIEALID NFQS+YECC
Sbjct: 97  HLLGIIDPFSDFSATNFSDMAMLSLDTILGRDRLPIIAGGSNSYIEALIDSNFQSKYECC 156

Query: 121 FLWVDVSMPVLCSFVSKRVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRY 180
           FLWVDVSMP+L SFVSKRVDKMVANGMV+EA++FFDPA DYSRGIKRAIGVPEFDSYFRY
Sbjct: 157 FLWVDVSMPILHSFVSKRVDKMVANGMVNEAKEFFDPAGDYSRGIKRAIGVPEFDSYFRY 216

Query: 181 KSLLDEETQDKLLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGK 240
           +SLLD+ETQ+KLLQ+AISKVK +TC+LA RQ+GKIQRL+N+KGWKIH+LDATEAF KQGK
Sbjct: 217 RSLLDDETQEKLLQEAISKVKKYTCKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGK 276

Query: 241 ESEEAWNKLVAAPSMAIIEKFLHQNVFT 268
           E+EE WNKLV  PS  IIE+FLH NV T
Sbjct: 277 EAEEEWNKLVTVPSTTIIEQFLHSNVIT 304

BLAST of MC09g0697 vs. ExPASy TrEMBL
Match: A0A0A0K919 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G392940 PE=4 SV=1)

HSP 1 Score: 457 bits (1177), Expect = 2.04e-160
Identity = 225/266 (84.59%), Postives = 247/266 (92.86%), Query Frame = 0

Query: 1   QKEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPH 60
           QKEKVVIVIG TGTGKSRLSIDIATRF SEVINSDKMQVY GLD+VTNK+TKQEQ GVPH
Sbjct: 37  QKEKVVIVIGPTGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPH 96

Query: 61  HLLGTINPFYDFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECC 120
           HLLG INPF DFSA++FSDMA LSLDAI++ DRLPIIAGGSNSYIEALID NF+ RYECC
Sbjct: 97  HLLGIINPFLDFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDSNFRLRYECC 156

Query: 121 FLWVDVSMPVLCSFVSKRVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRY 180
           FLWVDVSM VL SFVS+RVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRY
Sbjct: 157 FLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRY 216

Query: 181 KSLLDEETQDKLLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGK 240
           +SLLDEE Q+KLL+++I+KVK+HTC+LALRQLGKIQRL+NVKGW IH+LDATEAFTKQGK
Sbjct: 217 RSLLDEEAQEKLLEESIAKVKEHTCKLALRQLGKIQRLRNVKGWNIHRLDATEAFTKQGK 276

Query: 241 ESEEAWNKLVAAPSMAIIEKFLHQNV 266
           E++EAWNK V  PS  II++FLHQNV
Sbjct: 277 EADEAWNKHVTIPSTTIIDQFLHQNV 302

BLAST of MC09g0697 vs. ExPASy TrEMBL
Match: A0A5D3DBM7 (Adenylate isopentenyltransferase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold328G00080 PE=4 SV=1)

HSP 1 Score: 452 bits (1162), Expect = 3.93e-158
Identity = 221/265 (83.40%), Postives = 245/265 (92.45%), Query Frame = 0

Query: 1   QKEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPH 60
           QKEKVV VIGATGTGKSRLSIDIATRF SEVINSDKMQVY GLD+VTNK+TKQEQ GVPH
Sbjct: 37  QKEKVVFVIGATGTGKSRLSIDIATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPH 96

Query: 61  HLLGTINPFYDFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECC 120
           HLLG INPF DFSA++FSDMA LSLDAI++ DRLPIIAGGSNSYIEALID NF+ +YECC
Sbjct: 97  HLLGIINPFLDFSASNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDSNFRLKYECC 156

Query: 121 FLWVDVSMPVLCSFVSKRVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRY 180
           FLWVDVSM VL SFVS+RVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFR 
Sbjct: 157 FLWVDVSMQVLQSFVSERVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRC 216

Query: 181 KSLLDEETQDKLLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGK 240
           +SLLDEE Q+KLL+++I+KVK+HTC+LAL+QLGKIQRL+N+KGW IH+LDATEAFTKQGK
Sbjct: 217 RSLLDEEAQEKLLEESIAKVKEHTCKLALKQLGKIQRLRNIKGWNIHRLDATEAFTKQGK 276

Query: 241 ESEEAWNKLVAAPSMAIIEKFLHQN 265
           E+EEAWNKLV  PS  II++FLH N
Sbjct: 277 EAEEAWNKLVTIPSTTIIDQFLHPN 301

BLAST of MC09g0697 vs. TAIR 10
Match: AT5G19040.1 (isopentenyltransferase 5 )

HSP 1 Score: 311.6 bits (797), Expect = 6.1e-85
Identity = 159/267 (59.55%), Postives = 206/267 (77.15%), Query Frame = 0

Query: 1   QKEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPH 60
           +K+KVV V+GATGTGKSRL+ID+ATRF +E++NSDK+QVY+GLD+VTNKVT +E  GVPH
Sbjct: 31  RKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDIVTNKVTPEESLGVPH 90

Query: 61  HLLGTINPFY-DFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDP--NFQSRY 120
           HLLGT++  Y DF+A DF   A  ++++I  RDR+PIIAGGSNSYIEAL++   +F+ RY
Sbjct: 91  HLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAGGSNSYIEALVNDCVDFRLRY 150

Query: 121 ECCFLWVDVSMPVLCSFVSKRVDKMVANGMVDEAEKFFDP-ARDYSRGIKRAIGVPEFDS 180
            CCFLWVDVS PVL SFVS+RVDKMV  G+VDE  + FDP + DYS GI+RAIGVPE D 
Sbjct: 151 NCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDPSSSDYSAGIRRAIGVPELDE 210

Query: 181 YFRYKSL-LDEETQDKLLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWKIHQLDATEAF 240
           + R +      ET ++LL+ AI K+K++TC LA RQL KIQRL     W +H++DATE F
Sbjct: 211 FLRSEMRNYPAETTERLLETAIEKIKENTCLLACRQLQKIQRLYKQWKWNMHRVDATEVF 270

Query: 241 TKQGKESEEAWNKLVAAPSMAIIEKFL 263
            ++G+E++EAW+  VA PS   +EKFL
Sbjct: 271 LRRGEEADEAWDNSVAHPSALAVEKFL 297

BLAST of MC09g0697 vs. TAIR 10
Match: AT3G63110.1 (isopentenyltransferase 3 )

HSP 1 Score: 302.4 bits (773), Expect = 3.7e-82
Identity = 148/267 (55.43%), Postives = 202/267 (75.66%), Query Frame = 0

Query: 2   KEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPHH 61
           K+KVV+++GATGTGKSRLS+DIATRF +E+INSDK+QV++GLD+VTNK+T +E  GVPHH
Sbjct: 40  KDKVVVIMGATGTGKSRLSVDIATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHH 99

Query: 62  LLGTINPFYDFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDP---NFQSRYE 121
           LLG + P  D +A ++  MA LS++++  R +LPII GGSNSY+EAL+D     F+SRY+
Sbjct: 100 LLGVLPPEADLTAANYCHMANLSIESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYD 159

Query: 122 CCFLWVDVSMPVLCSFVSKRVDKMVANGMVDEAEKFFDPAR-DYSRGIKRAIGVPEFDSY 181
           CCFLWVDV++PVL  FVS+RVDKMV +GMV+E  +FFD +  DYSRGIK+AIG PEFD +
Sbjct: 160 CCFLWVDVALPVLHGFVSERVDKMVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRF 219

Query: 182 FRYKSLLDEETQDKLLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTK 241
           FR +  L+ E +++LL   + ++K +T  LA RQ  KI+RL+ VK W I ++DAT  FTK
Sbjct: 220 FRNEQFLNVEDREELLSKVLEEIKRNTFELACRQREKIERLRKVKKWSIQRVDATPVFTK 279

Query: 242 QGK--ESEEAWNKLVAAPSMAIIEKFL 263
           +    ++  AW +LVA PS   + +FL
Sbjct: 280 RRSKMDANVAWERLVAGPSTDTVSRFL 306

BLAST of MC09g0697 vs. TAIR 10
Match: AT3G23630.1 (isopentenyltransferase 7 )

HSP 1 Score: 281.2 bits (718), Expect = 8.8e-76
Identity = 145/271 (53.51%), Postives = 201/271 (74.17%), Query Frame = 0

Query: 2   KEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPHH 61
           KEKV+ V+GATG+GKSRL+ID+ATRF  E+INSDK+Q+Y+GLDV+TNKVT +E +GVPHH
Sbjct: 33  KEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLTNKVTPKECRGVPHH 92

Query: 62  LLGTI-NPFYDFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDPN---FQSRY 121
           LLG   +   + +AT +S +A  ++  ++A ++LPI+AGGSNSYIEAL++ +     + Y
Sbjct: 93  LLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIEALVNHSSGFLLNNY 152

Query: 122 ECCFLWVDVSMPVLCSFVSKRVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSY 181
           +CCF+WVDVS+PVL SFVSKRVD+M+  G+++E  + F+P  +YS GI+RAIGVPE   Y
Sbjct: 153 DCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNPKANYSVGIRRAIGVPELHEY 212

Query: 182 FRYKSLLDEETQDKLLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWK--IHQLDATEAF 241
            R +SL+D  T+ K+L  A+  +K +T  LA RQL KIQRL   K WK  +H++DATE F
Sbjct: 213 LRNESLVDRATKSKMLDVAVKNIKKNTEILACRQLKKIQRLH--KKWKMSMHRVDATEVF 272

Query: 242 TKQG-KESEEAWNKLVAAPSMAIIEKFLHQN 266
            K+  +E +EAW  LVA PS  I++KF + N
Sbjct: 273 LKRNVEEQDEAWENLVARPSERIVDKFYNNN 301

BLAST of MC09g0697 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 242.3 bits (617), Expect = 4.5e-64
Identity = 132/296 (44.59%), Postives = 194/296 (65.54%), Query Frame = 0

Query: 1   QKEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPH 60
           +K+KVV+++GATG GKSRLS+D+ATRF SE+INSDK+QVYEGL++ TN++T Q+++GVPH
Sbjct: 63  RKDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPH 122

Query: 61  HLLGTINPFY-DFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALI----DPNFQS 120
           HLLG INP + + +A +F   A   +  I +R ++PIIAGGSNS++ AL+    DP F  
Sbjct: 123 HLLGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDP 182

Query: 121 ------------RYECCFLWVDVSMPVLCSFVSKRVDKMVANGMVDEAEKFFDPAR---D 180
                       RYECCF+WVDVS  VL  ++ +RVD+M+ +GM +E  +F+DP +   +
Sbjct: 183 FSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLE 242

Query: 181 YSRGIKRAIGVPEFDSYFR----YKSLLDEETQDKLLQD-AISKVKDHTCRLALRQLGKI 240
              GI++AIGVPEFD YF+     K ++  +   K   D A+  +K +T  LA RQ+ KI
Sbjct: 243 TRFGIRKAIGVPEFDGYFKEYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKI 302

Query: 241 QRLKNVKGWKIHQLDATEAF------TKQGKESEEAWNKLVAAPSMAIIEKFLHQN 266
           + LK+  GW+I ++DAT +F      +   K+  E W + V  PS+ I+++ L QN
Sbjct: 303 EMLKDA-GWEIERVDATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIVKRHLVQN 357

BLAST of MC09g0697 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 231.9 bits (590), Expect = 6.1e-61
Identity = 132/291 (45.36%), Postives = 188/291 (64.60%), Query Frame = 0

Query: 2   KEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPHH 61
           K+KVV+++GATG+GKS LSID+ATRFS E++NSDK+Q Y+GL V TN+++  E+ GVPHH
Sbjct: 42  KQKVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHH 101

Query: 62  LLGTINP-FYDFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALI----DPN---F 121
           LLG + P   + + ++F  +A  S+  I AR  LPIIAGGSNS+I AL+    DP    F
Sbjct: 102 LLGELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSFIHALLVDRFDPKTYPF 161

Query: 122 QS--------RYECCFLWVDVSMPVLCSFVSKRVDKMVANGMVDEAEKFFDPARDYS--- 181
            S        RYECCFLWVDVS+ VL  ++SKRVD+M+ +GM +E   F+DP    S   
Sbjct: 162 SSETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGSAIR 221

Query: 182 -RGIKRAIGVPEFDSYFRYKSLLDEETQDKLLQ----------DAISKVKDHTCRLALRQ 241
             GI + IG+PEFD YF   SL   E + K+ +          +A+ ++K++T RLA +Q
Sbjct: 222 AHGIHKTIGIPEFDRYF---SLYPPERKQKMSEWDQARKGAYDEAVQEIKENTWRLAKKQ 281

Query: 242 LGKIQRLKNVKGWKIHQLDATEAFTKQGKESEEAWNKLVAAPSMAIIEKFL 263
           + +I +LK+  GW I +LDAT +F   G+ S E W+  V   S+ ++++FL
Sbjct: 282 IERIMKLKS-SGWDIQRLDATPSF---GRSSREIWDNTVLDESIKVVKRFL 325

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94ID28.6e-8459.55Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q93WC95.2e-8155.43Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q94ID11.2e-7453.51Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=370... [more]
Q5GHF71.3e-6343.89Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1[more]
Q94ID36.4e-6344.59Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Match NameE-valueIdentityDescription
XP_022150248.13.71e-191100.00adenylate isopentenyltransferase 3, chloroplastic [Momordica charantia][more]
XP_038899604.11.80e-16184.59adenylate isopentenyltransferase 3, chloroplastic [Benincasa hispida][more]
KAG7025533.12.56e-16183.96Adenylate isopentenyltransferase 3, chloroplastic, partial [Cucurbita argyrosper... [more]
XP_023514910.11.04e-16083.58adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita pepo subsp. pe... [more]
XP_022960175.12.97e-16083.96adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1D8Y21.80e-191100.00adenylate isopentenyltransferase 3, chloroplastic OS=Momordica charantia OX=3673... [more]
A0A6J1H8C91.44e-16083.96adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita moschata OX=... [more]
A0A6J1KSJ22.04e-16083.58adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita maxima OX=36... [more]
A0A0A0K9192.04e-16084.59Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G392940 PE=4 SV=1[more]
A0A5D3DBM73.93e-15883.40Adenylate isopentenyltransferase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
Match NameE-valueIdentityDescription
AT5G19040.16.1e-8559.55isopentenyltransferase 5 [more]
AT3G63110.13.7e-8255.43isopentenyltransferase 3 [more]
AT3G23630.18.8e-7653.51isopentenyltransferase 7 [more]
AT1G68460.14.5e-6444.59isopentenyltransferase 1 [more]
AT3G19160.16.1e-6145.36ATP/ADP isopentenyltransferases [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF01715IPPTcoord: 38..110
e-value: 1.2E-20
score: 74.3
coord: 115..213
e-value: 3.4E-11
score: 43.3
NoneNo IPR availableGENE3D1.10.287.890Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domaincoord: 128..226
e-value: 1.9E-108
score: 363.7
NoneNo IPR availablePANTHERPTHR11088:SF74ADENYLATE ISOPENTENYLTRANSFERASE 5, CHLOROPLASTICcoord: 1..265
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 2..262
e-value: 1.9E-108
score: 363.7
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 3..155
IPR039657DimethylallyltransferasePANTHERPTHR11088TRNA DIMETHYLALLYLTRANSFERASEcoord: 1..265

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g0697.1MC09g0697.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009691 cytokinin biosynthetic process
biological_process GO:0006400 tRNA modification
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005634 nucleus
cellular_component GO:0009536 plastid
molecular_function GO:0009824 AMP dimethylallyltransferase activity
molecular_function GO:0052381 tRNA dimethylallyltransferase activity