MC09g0201 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g0201
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionMidasin
LocationMC09: 1786023 .. 1786670 (-)
RNA-Seq ExpressionMC09g0201
SyntenyMC09g0201
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCATTGACGGGAGATTTAAGCTTGAATCCGCACTGGAAAGATTTCTTTACCGTTGTCCAGCTCTTACACATCTCTCACCGTTTGATATGCTTATCAAAGAGGTATGTTTTCCCAATATTGCATATAATGTTTGGTAATTTTGAGAGCATGATTCTCAAATGGTATTTGAAATACCACAACAACGTAGCAATTTTAATATCAGTCCTTTTGACCGTTCCTATGATCGATATGAGATTACTGCCGGGAATTGAAGGATTTGTTTCTCTGCCTCTTATTATATCTTAACTCTCCTTTTTGTACTCTGGCTTTTATAGGGATATGCAGTGACTGAAGAGGAAGCGTTGAGTATTGCAGCAGAGCTGTTTCTGAATCCAAATCATTCAATAACTTTGTTAGGTTGTTTCCGCCCATTAGCTCAAAAGATTCTGGTCAGAGTTGTAGGATTGCTAAGACTTGTGCCCAATTTGAGATCAAACTCAGATATCACAGCTTCCAAAATTCACGAAGAGGACCTGAATGGAGTTGTCAATATAATTTCGCTTTATGACCAAAGAGGGAGGGGGCTTGATCTTCACGAGCTTGCTTGTCTAGCATTTTGTCGTGCCCTTGATTTGGCCCCCTTTTTATTAGGGTTTGTCACTCAT

mRNA sequence

ATGGCCATTGACGGGAGATTTAAGCTTGAATCCGCACTGGAAAGATTTCTTTACCGTTGTCCAGCTCTTACACATCTCTCACCGTTTGATATGCTTATCAAAGAGGGATATGCAGTGACTGAAGAGGAAGCGTTGAGTATTGCAGCAGAGCTGTTTCTGAATCCAAATCATTCAATAACTTTGTTAGGTTGTTTCCGCCCATTAGCTCAAAAGATTCTGGTCAGAGTTGTAGGATTGCTAAGACTTGTGCCCAATTTGAGATCAAACTCAGATATCACAGCTTCCAAAATTCACGAAGAGGACCTGAATGGAGTTGTCAATATAATTTCGCTTTATGACCAAAGAGGGAGGGGGCTTGATCTTCACGAGCTTGCTTGTCTAGCATTTTGTCGTGCCCTTGATTTGGCCCCCTTTTTATTAGGGTTTGTCACTCAT

Coding sequence (CDS)

ATGGCCATTGACGGGAGATTTAAGCTTGAATCCGCACTGGAAAGATTTCTTTACCGTTGTCCAGCTCTTACACATCTCTCACCGTTTGATATGCTTATCAAAGAGGGATATGCAGTGACTGAAGAGGAAGCGTTGAGTATTGCAGCAGAGCTGTTTCTGAATCCAAATCATTCAATAACTTTGTTAGGTTGTTTCCGCCCATTAGCTCAAAAGATTCTGGTCAGAGTTGTAGGATTGCTAAGACTTGTGCCCAATTTGAGATCAAACTCAGATATCACAGCTTCCAAAATTCACGAAGAGGACCTGAATGGAGTTGTCAATATAATTTCGCTTTATGACCAAAGAGGGAGGGGGCTTGATCTTCACGAGCTTGCTTGTCTAGCATTTTGTCGTGCCCTTGATTTGGCCCCCTTTTTATTAGGGTTTGTCACTCAT

Protein sequence

MAIDGRFKLESALERFLYRCPALTHLSPFDMLIKEGYAVTEEEALSIAAELFLNPNHSITLLGCFRPLAQKILVRVVGLLRLVPNLRSNSDITASKIHEEDLNGVVNIISLYDQRGRGLDLHELACLAFCRALDLAPFLLGFVTH
Homology
BLAST of MC09g0201 vs. ExPASy Swiss-Prot
Match: A0A1P8AUY4 (Midasin OS=Arabidopsis thaliana OX=3702 GN=MDN1 PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.0e-22
Identity = 60/146 (41.10%), Postives = 87/146 (59.59%), Query Frame = 0

Query: 1   MAIDGRFKLESALERFLYRCPALTHLSPF-DMLIKEGYAVTEEEALSIAAELFLNPNHSI 60
           MAIDG F L+ ALE F  RCP +     F  +L K G  V  EE +    + FL+P  ++
Sbjct: 1   MAIDGSFNLKLALETFSVRCPKVAAFPCFTSILSKGGEVVDNEEVIHALGDAFLHPEFTV 60

Query: 61  TLLGCFRPLAQKILVRVVGLLRLVPNLRSNSDIT--ASKIHEEDLNGVVNIISLYDQRGR 120
            L+ CF P+ + ++ RVVGLLRLV +L+S+ D +   S + +  +   +++I  Y +RG+
Sbjct: 61  PLVHCFLPIIRNVVDRVVGLLRLVDDLKSSIDYSDDVSSVLDNAMTEGISVIDFYVRRGQ 120

Query: 121 GLDLHELACLAFCRALDLAPFLLGFV 144
            L+LHE ACLAF RAL     LLG +
Sbjct: 121 RLELHECACLAFSRALHFNTSLLGSI 146

BLAST of MC09g0201 vs. NCBI nr
Match: XP_022152480.1 (midasin isoform X2 [Momordica charantia])

HSP 1 Score: 278 bits (710), Expect = 2.21e-83
Identity = 141/141 (100.00%), Postives = 141/141 (100.00%), Query Frame = 0

Query: 1   MAIDGRFKLESALERFLYRCPALTHLSPFDMLIKEGYAVTEEEALSIAAELFLNPNHSIT 60
           MAIDGRFKLESALERFLYRCPALTHLSPFDMLIKEGYAVTEEEALSIAAELFLNPNHSIT
Sbjct: 1   MAIDGRFKLESALERFLYRCPALTHLSPFDMLIKEGYAVTEEEALSIAAELFLNPNHSIT 60

Query: 61  LLGCFRPLAQKILVRVVGLLRLVPNLRSNSDITASKIHEEDLNGVVNIISLYDQRGRGLD 120
           LLGCFRPLAQKILVRVVGLLRLVPNLRSNSDITASKIHEEDLNGVVNIISLYDQRGRGLD
Sbjct: 61  LLGCFRPLAQKILVRVVGLLRLVPNLRSNSDITASKIHEEDLNGVVNIISLYDQRGRGLD 120

Query: 121 LHELACLAFCRALDLAPFLLG 141
           LHELACLAFCRALDLAPFLLG
Sbjct: 121 LHELACLAFCRALDLAPFLLG 141

BLAST of MC09g0201 vs. NCBI nr
Match: XP_022152472.1 (midasin isoform X1 [Momordica charantia])

HSP 1 Score: 278 bits (710), Expect = 2.22e-83
Identity = 141/141 (100.00%), Postives = 141/141 (100.00%), Query Frame = 0

Query: 1   MAIDGRFKLESALERFLYRCPALTHLSPFDMLIKEGYAVTEEEALSIAAELFLNPNHSIT 60
           MAIDGRFKLESALERFLYRCPALTHLSPFDMLIKEGYAVTEEEALSIAAELFLNPNHSIT
Sbjct: 1   MAIDGRFKLESALERFLYRCPALTHLSPFDMLIKEGYAVTEEEALSIAAELFLNPNHSIT 60

Query: 61  LLGCFRPLAQKILVRVVGLLRLVPNLRSNSDITASKIHEEDLNGVVNIISLYDQRGRGLD 120
           LLGCFRPLAQKILVRVVGLLRLVPNLRSNSDITASKIHEEDLNGVVNIISLYDQRGRGLD
Sbjct: 61  LLGCFRPLAQKILVRVVGLLRLVPNLRSNSDITASKIHEEDLNGVVNIISLYDQRGRGLD 120

Query: 121 LHELACLAFCRALDLAPFLLG 141
           LHELACLAFCRALDLAPFLLG
Sbjct: 121 LHELACLAFCRALDLAPFLLG 141

BLAST of MC09g0201 vs. NCBI nr
Match: XP_023548564.1 (midasin [Cucurbita pepo subsp. pepo])

HSP 1 Score: 239 bits (611), Expect = 4.68e-70
Identity = 121/141 (85.82%), Postives = 131/141 (92.91%), Query Frame = 0

Query: 1   MAIDGRFKLESALERFLYRCPALTHLSPFDMLIKEGYAVTEEEALSIAAELFLNPNHSIT 60
           MAIDG FKLE+ALERFL RCPALTHLS FD LIK+GYAVTEEE LS AAELFLNPN+SI 
Sbjct: 1   MAIDGSFKLEAALERFLSRCPALTHLSLFDTLIKKGYAVTEEEVLSTAAELFLNPNYSIC 60

Query: 61  LLGCFRPLAQKILVRVVGLLRLVPNLRSNSDITASKIHEEDLNGVVNIISLYDQRGRGLD 120
           L+GCFRPLAQKI+ RV+GLLRLVPNLRSNS+IT S+IHEE+LNGVVNIIS Y+QRGRGLD
Sbjct: 61  LIGCFRPLAQKIVDRVIGLLRLVPNLRSNSNITDSEIHEEELNGVVNIISFYEQRGRGLD 120

Query: 121 LHELACLAFCRALDLAPFLLG 141
           LHELACLAFCRALDLAPFLLG
Sbjct: 121 LHELACLAFCRALDLAPFLLG 141

BLAST of MC09g0201 vs. NCBI nr
Match: KAG7014001.1 (Midasin [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 238 bits (606), Expect = 2.21e-69
Identity = 120/141 (85.11%), Postives = 130/141 (92.20%), Query Frame = 0

Query: 1   MAIDGRFKLESALERFLYRCPALTHLSPFDMLIKEGYAVTEEEALSIAAELFLNPNHSIT 60
           MAIDG FKLE+ALERFL RCPALTHLS FD LIK+GYAVTEEE LS AAELFLNPN+SI 
Sbjct: 1   MAIDGSFKLEAALERFLSRCPALTHLSLFDTLIKKGYAVTEEEVLSTAAELFLNPNYSIC 60

Query: 61  LLGCFRPLAQKILVRVVGLLRLVPNLRSNSDITASKIHEEDLNGVVNIISLYDQRGRGLD 120
           L+GCFRPLAQ I+ RV+GLLRLVPNLRSNS+IT S+IHEE+LNGVVNIIS Y+QRGRGLD
Sbjct: 61  LIGCFRPLAQNIVDRVIGLLRLVPNLRSNSNITDSEIHEEELNGVVNIISFYEQRGRGLD 120

Query: 121 LHELACLAFCRALDLAPFLLG 141
           LHELACLAFCRALDLAPFLLG
Sbjct: 121 LHELACLAFCRALDLAPFLLG 141

BLAST of MC09g0201 vs. NCBI nr
Match: KAG6575456.1 (Midasin, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 238 bits (606), Expect = 2.21e-69
Identity = 120/141 (85.11%), Postives = 130/141 (92.20%), Query Frame = 0

Query: 1   MAIDGRFKLESALERFLYRCPALTHLSPFDMLIKEGYAVTEEEALSIAAELFLNPNHSIT 60
           MAIDG FKLE+ALERFL RCPALTHLS FD LIK+GYAVTEEE LS AAELFLNPN+SI 
Sbjct: 1   MAIDGSFKLEAALERFLSRCPALTHLSLFDTLIKKGYAVTEEEVLSTAAELFLNPNYSIC 60

Query: 61  LLGCFRPLAQKILVRVVGLLRLVPNLRSNSDITASKIHEEDLNGVVNIISLYDQRGRGLD 120
           L+GCFRPLAQ I+ RV+GLLRLVPNLRSNS+IT S+IHEE+LNGVVNIIS Y+QRGRGLD
Sbjct: 61  LIGCFRPLAQNIVDRVIGLLRLVPNLRSNSNITDSEIHEEELNGVVNIISFYEQRGRGLD 120

Query: 121 LHELACLAFCRALDLAPFLLG 141
           LHELACLAFCRALDLAPFLLG
Sbjct: 121 LHELACLAFCRALDLAPFLLG 141

BLAST of MC09g0201 vs. ExPASy TrEMBL
Match: A0A6J1DE15 (Midasin OS=Momordica charantia OX=3673 GN=LOC111020190 PE=3 SV=1)

HSP 1 Score: 278 bits (710), Expect = 1.07e-83
Identity = 141/141 (100.00%), Postives = 141/141 (100.00%), Query Frame = 0

Query: 1   MAIDGRFKLESALERFLYRCPALTHLSPFDMLIKEGYAVTEEEALSIAAELFLNPNHSIT 60
           MAIDGRFKLESALERFLYRCPALTHLSPFDMLIKEGYAVTEEEALSIAAELFLNPNHSIT
Sbjct: 1   MAIDGRFKLESALERFLYRCPALTHLSPFDMLIKEGYAVTEEEALSIAAELFLNPNHSIT 60

Query: 61  LLGCFRPLAQKILVRVVGLLRLVPNLRSNSDITASKIHEEDLNGVVNIISLYDQRGRGLD 120
           LLGCFRPLAQKILVRVVGLLRLVPNLRSNSDITASKIHEEDLNGVVNIISLYDQRGRGLD
Sbjct: 61  LLGCFRPLAQKILVRVVGLLRLVPNLRSNSDITASKIHEEDLNGVVNIISLYDQRGRGLD 120

Query: 121 LHELACLAFCRALDLAPFLLG 141
           LHELACLAFCRALDLAPFLLG
Sbjct: 121 LHELACLAFCRALDLAPFLLG 141

BLAST of MC09g0201 vs. ExPASy TrEMBL
Match: A0A6J1DGC5 (Midasin OS=Momordica charantia OX=3673 GN=LOC111020190 PE=3 SV=1)

HSP 1 Score: 278 bits (710), Expect = 1.07e-83
Identity = 141/141 (100.00%), Postives = 141/141 (100.00%), Query Frame = 0

Query: 1   MAIDGRFKLESALERFLYRCPALTHLSPFDMLIKEGYAVTEEEALSIAAELFLNPNHSIT 60
           MAIDGRFKLESALERFLYRCPALTHLSPFDMLIKEGYAVTEEEALSIAAELFLNPNHSIT
Sbjct: 1   MAIDGRFKLESALERFLYRCPALTHLSPFDMLIKEGYAVTEEEALSIAAELFLNPNHSIT 60

Query: 61  LLGCFRPLAQKILVRVVGLLRLVPNLRSNSDITASKIHEEDLNGVVNIISLYDQRGRGLD 120
           LLGCFRPLAQKILVRVVGLLRLVPNLRSNSDITASKIHEEDLNGVVNIISLYDQRGRGLD
Sbjct: 61  LLGCFRPLAQKILVRVVGLLRLVPNLRSNSDITASKIHEEDLNGVVNIISLYDQRGRGLD 120

Query: 121 LHELACLAFCRALDLAPFLLG 141
           LHELACLAFCRALDLAPFLLG
Sbjct: 121 LHELACLAFCRALDLAPFLLG 141

BLAST of MC09g0201 vs. ExPASy TrEMBL
Match: A0A6J1GQ37 (Midasin OS=Cucurbita moschata OX=3662 GN=LOC111456078 PE=3 SV=1)

HSP 1 Score: 237 bits (605), Expect = 1.46e-69
Identity = 120/141 (85.11%), Postives = 130/141 (92.20%), Query Frame = 0

Query: 1   MAIDGRFKLESALERFLYRCPALTHLSPFDMLIKEGYAVTEEEALSIAAELFLNPNHSIT 60
           MAIDG FKLE+ALERFL RCPALTHLS FD LIK+GYAVTEEE LS AAELFLNPN+SI 
Sbjct: 1   MAIDGSFKLEAALERFLSRCPALTHLSLFDTLIKKGYAVTEEEVLSTAAELFLNPNYSIC 60

Query: 61  LLGCFRPLAQKILVRVVGLLRLVPNLRSNSDITASKIHEEDLNGVVNIISLYDQRGRGLD 120
           L+GCFRPLAQKI+ RV+GLLRLVPNLRSNS+I  S+IHEE+LNGVVNIIS Y+QRGRGLD
Sbjct: 61  LIGCFRPLAQKIVDRVIGLLRLVPNLRSNSNIIDSEIHEEELNGVVNIISFYEQRGRGLD 120

Query: 121 LHELACLAFCRALDLAPFLLG 141
           LHELACLAFCRALDLAPFLLG
Sbjct: 121 LHELACLAFCRALDLAPFLLG 141

BLAST of MC09g0201 vs. ExPASy TrEMBL
Match: A0A6J1JXY2 (Midasin OS=Cucurbita maxima OX=3661 GN=LOC111488513 PE=3 SV=1)

HSP 1 Score: 235 bits (600), Expect = 6.86e-69
Identity = 118/141 (83.69%), Postives = 129/141 (91.49%), Query Frame = 0

Query: 1   MAIDGRFKLESALERFLYRCPALTHLSPFDMLIKEGYAVTEEEALSIAAELFLNPNHSIT 60
           MAIDG FKLE+ALERFL RCPALTHLS FD LIK+GYAVTEEE LS AAE+FLNPN+SI 
Sbjct: 1   MAIDGSFKLEAALERFLSRCPALTHLSLFDTLIKKGYAVTEEEVLSTAAEMFLNPNYSIC 60

Query: 61  LLGCFRPLAQKILVRVVGLLRLVPNLRSNSDITASKIHEEDLNGVVNIISLYDQRGRGLD 120
           L+GCFRPLAQKI+ RV+GLLRLVPNLRSNS IT S+IHEE+LNGV+NIIS Y+QRGRGLD
Sbjct: 61  LIGCFRPLAQKIVDRVIGLLRLVPNLRSNSKITDSEIHEEELNGVINIISFYEQRGRGLD 120

Query: 121 LHELACLAFCRALDLAPFLLG 141
           LHELAC AFCRALDLAPFLLG
Sbjct: 121 LHELACQAFCRALDLAPFLLG 141

BLAST of MC09g0201 vs. ExPASy TrEMBL
Match: A0A0A0KAY4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G450650 PE=4 SV=1)

HSP 1 Score: 219 bits (557), Expect = 1.27e-67
Identity = 109/145 (75.17%), Postives = 128/145 (88.28%), Query Frame = 0

Query: 1   MAIDGRFKLESALERFLYRCPALTHLSPFDMLIKEGYAVTEEEALSIAAELFLNPNHSIT 60
           MAIDG FKLESA+ERFL RCP+LTH SPF+ +I++GY VTEEE LS+ AELFLNPN+S+ 
Sbjct: 1   MAIDGSFKLESAVERFLSRCPSLTHQSPFETIIRKGYGVTEEEVLSLLAELFLNPNYSVC 60

Query: 61  LLGCFRPLAQKILVRVVGLLRLVPNLRSNSDITASKIHEEDLNGVVNIISLYDQRGRGLD 120
           LLGCFRP+AQKI+ RV+GLLR VPNLRSNS  T S+IHE++L+GVVNIIS YDQRGRG+D
Sbjct: 61  LLGCFRPVAQKIVDRVIGLLRHVPNLRSNS--TYSEIHEKELDGVVNIISFYDQRGRGVD 120

Query: 121 LHELACLAFCRALDLAPFLLGFVTH 145
           LHELACLAFCRALDLAPFLL  + +
Sbjct: 121 LHELACLAFCRALDLAPFLLRTILN 143

BLAST of MC09g0201 vs. TAIR 10
Match: AT1G67120.1 (ATPases;nucleotide binding;ATP binding;nucleoside-triphosphatases;transcription factor binding )

HSP 1 Score: 107.8 bits (268), Expect = 7.2e-24
Identity = 60/146 (41.10%), Postives = 87/146 (59.59%), Query Frame = 0

Query: 1   MAIDGRFKLESALERFLYRCPALTHLSPF-DMLIKEGYAVTEEEALSIAAELFLNPNHSI 60
           MAIDG F L+ ALE F  RCP +     F  +L K G  V  EE +    + FL+P  ++
Sbjct: 1   MAIDGSFNLKLALETFSVRCPKVAAFPCFTSILSKGGEVVDNEEVIHALGDAFLHPEFTV 60

Query: 61  TLLGCFRPLAQKILVRVVGLLRLVPNLRSNSDIT--ASKIHEEDLNGVVNIISLYDQRGR 120
            L+ CF P+ + ++ RVVGLLRLV +L+S+ D +   S + +  +   +++I  Y +RG+
Sbjct: 61  PLVHCFLPIIRNVVDRVVGLLRLVDDLKSSIDYSDDVSSVLDNAMTEGISVIDFYVRRGQ 120

Query: 121 GLDLHELACLAFCRALDLAPFLLGFV 144
            L+LHE ACLAF RAL     LLG +
Sbjct: 121 RLELHECACLAFSRALHFNTSLLGSI 146

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A1P8AUY41.0e-2241.10Midasin OS=Arabidopsis thaliana OX=3702 GN=MDN1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022152480.12.21e-83100.00midasin isoform X2 [Momordica charantia][more]
XP_022152472.12.22e-83100.00midasin isoform X1 [Momordica charantia][more]
XP_023548564.14.68e-7085.82midasin [Cucurbita pepo subsp. pepo][more]
KAG7014001.12.21e-6985.11Midasin [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6575456.12.21e-6985.11Midasin, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A6J1DE151.07e-83100.00Midasin OS=Momordica charantia OX=3673 GN=LOC111020190 PE=3 SV=1[more]
A0A6J1DGC51.07e-83100.00Midasin OS=Momordica charantia OX=3673 GN=LOC111020190 PE=3 SV=1[more]
A0A6J1GQ371.46e-6985.11Midasin OS=Cucurbita moschata OX=3662 GN=LOC111456078 PE=3 SV=1[more]
A0A6J1JXY26.86e-6983.69Midasin OS=Cucurbita maxima OX=3661 GN=LOC111488513 PE=3 SV=1[more]
A0A0A0KAY41.27e-6775.17Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G450650 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G67120.17.2e-2441.10ATPases;nucleotide binding;ATP binding;nucleoside-triphosphatases;transcription ... [more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g0201.1MC09g0201.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000027 ribosomal large subunit assembly
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity