MC09g0195 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g0195
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionprotein BIG GRAIN 1-like B
LocationMC09: 1707126 .. 1708373 (-)
RNA-Seq ExpressionMC09g0195
SyntenyMC09g0195
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGTTTGGGACCAACAGATGCCAGAAGATCGACACGGCCGCCACCGGAGGCAGAATCCGTCATTTTCTTCTTCTCTTCTTGACGCCATTTATCGCTCCATCGACGAAACCAACGGTGTCCAGGGCGAAGAGCAGGTCGTCTTGTACAGAGAGACTATGAGGAAGAAGAAGCAGAGCATTGGCTTGGAAGACGGTGAAGATGATGGACTCAATTTCCGACGCGCTTGTATGATCGAGAAGTGGATGGAGAAGAAGGTGAGCGAGAAGGTCGTGGTTCGCCGGAGTTCTATGGCGGATTTCGATCGGAAGGCGAGGAATGTTCGAGATCGCGATCGCGATCGCAATCGTGGTTTTAAGAAATTGATGAATTCGAATTCGAGTTCGAGTTCCTCTGAATCCAGTTGTGGAGGGGGATTTTCTTCGTCCGAGTCGGAATCGAGGTCGTCTTCTTATACAATGCTGAGGCCGAGGCCGATAAGGACGGAGAAGCCTCAATTCGACGGCCATGGCGACGCGATTAAAGTTTCGGTCCCCAATCAGAAGCCGAAGCACGAAAACGGCTTCGTGAAGACGAAATCGAAAGCCCTAAAAATCTACGGCGATCTGAAGAAAGTGAAGCAGCCAATCTCTCCCGGCGGCCGCTTCGCGACCTTCCTGAACTCCCTCTTCAACGGCGGCAGTCCAAAGAAGCAAAAATTCACAAACTCCGCCCAACAGAACGACGCCTGTATGAGAAAATCCAAGTCCGCTCAAGGATCGACGTGTTCTTCCGCCTCCTCGTTCTCCAGGTCCTGTCTCAGCAAAACACCGTCGTCCGGCGAGAAACTCATCAACAATGGCAGCACAAAACGATCGGTCAGATTTTGCCCCGTGAGCGTGATACTAGACGAGGACTCTCGGCCTTGCGGACATAAAAGCCTGCAAGACGAACTAGGTCTAATGGCGGCAGCGACAACTCTGCGAAACGCTCTGAGGAACGACCATTGCCGAGCAGATGAAGCTACAGAGGATCTGATGAACAGTTACCAAAAGAAGATCAATGGCGATGTATATATAAACAGAGAAGAAGACGACGAAGATGAAGAGGAAGACGACGAAGAAGAAGAAGACGACGACGATGCAGTGAGTTGCTCGAGCTCTGATTTGTTCGAATTGGATAATCTCTCATCGATCGGAATCGAAAGGTATCGCCAAGAACTTCCAGTTTATGAAACAACGCATTTCAGTACGAATCAGGCCATCGCC

mRNA sequence

ATGGATGTTTGGGACCAACAGATGCCAGAAGATCGACACGGCCGCCACCGGAGGCAGAATCCGTCATTTTCTTCTTCTCTTCTTGACGCCATTTATCGCTCCATCGACGAAACCAACGGTGTCCAGGGCGAAGAGCAGGTCGTCTTTTCTATGGCGGATTTCGATCGGAAGGCGAGGAATGTTCGAGATCGCGATCGCGATCGCAATCGTGGTTTTAAGAAATTGATGAATTCGAATTCGAGTTCGAGTTCCTCTGAATCCAGTTGTGGAGGGGGATTTTCTTCGTCCGAGTCGGAATCGAGGTCGTCTTCTTATACAATGCTGAGGCCGAGGCCGATAAGGACGGAGAAGCCTCAATTCGACGGCCATGGCGACGCGATTAAAGTTTCGGTCCCCAATCAGAAGCCGAAGCACGAAAACGGCTTCGTGAAGACGAAATCGAAAGCCCTAAAAATCTACGGCGATCTGAAGAAAGTGAAGCAGCCAATCTCTCCCGGCGGCCGCTTCGCGACCTTCCTGAACTCCCTCTTCAACGGCGGCAGTCCAAAGAAGCAAAAATTCACAAACTCCGCCCAACAGAACGACGCCTGTATGAGAAAATCCAAGTCCGCTCAAGGATCGACGTGTTCTTCCGCCTCCTCGTTCTCCAGGTCCTGTCTCAGCAAAACACCGTCGTCCGGCGAGAAACTCATCAACAATGGCAGCACAAAACGATCGGTCAGATTTTGCCCCGTGAGCGTGATACTAGACGAGGACTCTCGGCCTTGCGGACATAAAAGCCTGCAAGACGAACTAGGTCTAATGGCGGCAGCGACAACTCTGCGAAACGCTCTGAGGAACGACCATTGCCGAGCAGATGAAGCTACAGAGGATCTGATGAACAGTTACCAAAAGAAGATCAATGGCGATGTATATATAAACAGAGAAGAAGACGACGAAGATGAAGAGGAAGACGACGAAGAAGAAGAAGACGACGACGATGCAGTGAGTTGCTCGAGCTCTGATTTGTTCGAATTGGATAATCTCTCATCGATCGGAATCGAAAGGTATCGCCAAGAACTTCCAGTTTATGAAACAACGCATTTCAGTACGAATCAGGCCATCGCC

Coding sequence (CDS)

ATGGATGTTTGGGACCAACAGATGCCAGAAGATCGACACGGCCGCCACCGGAGGCAGAATCCGTCATTTTCTTCTTCTCTTCTTGACGCCATTTATCGCTCCATCGACGAAACCAACGGTGTCCAGGGCGAAGAGCAGGTCGTCTTTTCTATGGCGGATTTCGATCGGAAGGCGAGGAATGTTCGAGATCGCGATCGCGATCGCAATCGTGGTTTTAAGAAATTGATGAATTCGAATTCGAGTTCGAGTTCCTCTGAATCCAGTTGTGGAGGGGGATTTTCTTCGTCCGAGTCGGAATCGAGGTCGTCTTCTTATACAATGCTGAGGCCGAGGCCGATAAGGACGGAGAAGCCTCAATTCGACGGCCATGGCGACGCGATTAAAGTTTCGGTCCCCAATCAGAAGCCGAAGCACGAAAACGGCTTCGTGAAGACGAAATCGAAAGCCCTAAAAATCTACGGCGATCTGAAGAAAGTGAAGCAGCCAATCTCTCCCGGCGGCCGCTTCGCGACCTTCCTGAACTCCCTCTTCAACGGCGGCAGTCCAAAGAAGCAAAAATTCACAAACTCCGCCCAACAGAACGACGCCTGTATGAGAAAATCCAAGTCCGCTCAAGGATCGACGTGTTCTTCCGCCTCCTCGTTCTCCAGGTCCTGTCTCAGCAAAACACCGTCGTCCGGCGAGAAACTCATCAACAATGGCAGCACAAAACGATCGGTCAGATTTTGCCCCGTGAGCGTGATACTAGACGAGGACTCTCGGCCTTGCGGACATAAAAGCCTGCAAGACGAACTAGGTCTAATGGCGGCAGCGACAACTCTGCGAAACGCTCTGAGGAACGACCATTGCCGAGCAGATGAAGCTACAGAGGATCTGATGAACAGTTACCAAAAGAAGATCAATGGCGATGTATATATAAACAGAGAAGAAGACGACGAAGATGAAGAGGAAGACGACGAAGAAGAAGAAGACGACGACGATGCAGTGAGTTGCTCGAGCTCTGATTTGTTCGAATTGGATAATCTCTCATCGATCGGAATCGAAAGGTATCGCCAAGAACTTCCAGTTTATGAAACAACGCATTTCAGTACGAATCAGGCCATCGCC

Protein sequence

MDVWDQQMPEDRHGRHRRQNPSFSSSLLDAIYRSIDETNGVQGEEQVVFSMADFDRKARNVRDRDRDRNRGFKKLMNSNSSSSSSESSCGGGFSSSESESRSSSYTMLRPRPIRTEKPQFDGHGDAIKVSVPNQKPKHENGFVKTKSKALKIYGDLKKVKQPISPGGRFATFLNSLFNGGSPKKQKFTNSAQQNDACMRKSKSAQGSTCSSASSFSRSCLSKTPSSGEKLINNGSTKRSVRFCPVSVILDEDSRPCGHKSLQDELGLMAAATTLRNALRNDHCRADEATEDLMNSYQKKINGDVYINREEDDEDEEEDDEEEEDDDDAVSCSSSDLFELDNLSSIGIERYRQELPVYETTHFSTNQAIA
Homology
BLAST of MC09g0195 vs. ExPASy Swiss-Prot
Match: Q9SLL2 (Protein BIG GRAIN 1-like B OS=Arabidopsis thaliana OX=3702 GN=At1g54200 PE=1 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 4.4e-62
Identity = 181/384 (47.14%), Postives = 234/384 (60.94%), Query Frame = 0

Query: 1   MDVWD-QQMPEDRHGRHR----RQNPSFSSSLLDAIYRSIDETNGVQGEEQVVFSMADFD 60
           MD WD   +P D   R R     ++PSFSS+LLD IYRSID+++      +        D
Sbjct: 1   MDPWDTYNIPLDHQHRLRNRRDHRHPSFSSTLLDQIYRSIDDSSTNSSSMRKTKHQNRED 60

Query: 61  RKARNVRDRDRDRNRGFKKLMNSNSSSSSSESSCGGGFSSSESE----SRSSSYTMLRPR 120
            +    R  D +R++  K +       SSS SS   GFSSSES+       SS  +  P+
Sbjct: 61  TRVSANRRDDFNRSKNLKTIEPVFFKHSSSSSSDSSGFSSSESDYFYRRSKSSPAISHPK 120

Query: 121 PIRTEKPQFDGHGDAIKVSVPNQKPKHENG-FVKTKSKALKIYGDLKKVKQPISPGGRFA 180
           PIRT   +F+      +   PN   K E+G F+KTKSKALKIY DLKKVKQPISPGGR A
Sbjct: 121 PIRTTVERFERSPQNHR---PNSSNKQEHGSFLKTKSKALKIYSDLKKVKQPISPGGRLA 180

Query: 181 TFLNSLFNG-GSPKKQKFTNSAQQNDACMRKSKSAQGSTCSSASSFSRSCLSKTPSSGEK 240
           TFLNS+F G G+ KK    N+   +      + S+  +TCSSASSFSRSCLSKTPSS EK
Sbjct: 181 TFLNSIFTGAGNTKKLNKINTTVTSTTAAAAAASST-TTCSSASSFSRSCLSKTPSSSEK 240

Query: 241 LINNGSTKRSVRFCPVSVILDEDSRPCGHKSLQ----DELGLMAAATTLRNALRNDHCRA 300
                 +KRSVRFCPV+VI DEDS    +K+ +    +E    +   TL N +  ++ R 
Sbjct: 241 ------SKRSVRFCPVNVIFDEDSSKYNNKNNKVYGNNEREYESIRHTLENRVMEENRRV 300

Query: 301 DEATEDLMNSYQKKINGDVYINREEDDEDEEEDDEEEEDDDDAVSCSSSDLFELDNLSSI 360
            EA ++L+ SYQKK       N+E  +   E  D+EE+DDDDA+SC+SSDLFELDNLS+I
Sbjct: 301 IEAAKELLRSYQKK-------NKEVIEVSVE--DDEEDDDDDALSCTSSDLFELDNLSAI 360

Query: 361 GIERYRQELPVYETTHFSTNQAIA 370
           GI+RYR+ELPVYETT  +TN+ I+
Sbjct: 361 GIDRYREELPVYETTRLNTNRIIS 365

BLAST of MC09g0195 vs. ExPASy Swiss-Prot
Match: Q9LVK2 (Protein BIG GRAIN 1-like A OS=Arabidopsis thaliana OX=3702 GN=At3g13980 PE=3 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 5.9e-51
Identity = 178/394 (45.18%), Postives = 222/394 (56.35%), Query Frame = 0

Query: 4   WDQQMPEDRHGRHRRQNPSFSSSLLDAIYRSIDETNGVQGEEQVVFSMADF-DRKARNVR 63
           W++      H     +NPSFSS+LLD IYRSID+++     E +         R A    
Sbjct: 5   WEKPKSSSHH-----RNPSFSSTLLDQIYRSIDDSSPPPPLESIKKKKHHHQQRNASLHE 64

Query: 64  DRD-----------RDRNRGFKK---LMNSNSSSSSSESSCGGGFSSSESE-----SRSS 123
           DR+            D  R  +K   L +SNSSSS S      GFSSSES+     S+SS
Sbjct: 65  DREISPIYHRRSIAADFERSRRKTDFLRHSNSSSSDS-----SGFSSSESDSFHGRSKSS 124

Query: 124 ----SYTMLRPRPIRTEKPQFDGHGDAIKVSVPNQKPKHENGFVKTKSKALKIYGDLKKV 183
               S +  +P+PIRT       H  A+      QKPK   GF++TKSKALKIY DLKKV
Sbjct: 125 ASPPSSSRQQPKPIRTSSVD---HSSAV------QKPKELGGFLRTKSKALKIYSDLKKV 184

Query: 184 KQPISPGGRFATFLNSLFNGG--SPKKQKFTNSAQQNDACMRKSKSAQGSTCSSASSFSR 243
           KQPISPGGR ATFLNSLF     +PKK K T +     A + +  S+  STCSSASSFSR
Sbjct: 185 KQPISPGGRLATFLNSLFTNAATNPKKHKKTTTV----AVVEEPHSS--STCSSASSFSR 244

Query: 244 SCLSKTPSSGEKLINNGSTKRSVRFCPVSVILDEDSRPCGHKSLQDELGLMAAATTLRNA 303
           SCLSKTPSS      +G +KRSVRFCPV+VILDEDS      +  +E            A
Sbjct: 245 SCLSKTPSS------SGKSKRSVRFCPVNVILDEDSSFTMPYAYNNE-----RLYDNNEA 304

Query: 304 LR-NDHCRADEATEDLMNSYQKKINGDVYINREEDDEDEEEDDEEEEDDDDAVSCSSSDL 363
            R  +H R  +A +DL+ +Y  K                  ++ EE+D+DDA SC+SSDL
Sbjct: 305 KRVEEHRRVIQAAKDLLRTYHNK----------NKVTTTNINNVEEDDEDDAASCASSDL 352

Query: 364 FELDNLSSIGIERYRQELPVYETTHF-STNQAIA 370
           FEL+NLS+IGIERYR+ELPVYETT   + N+ IA
Sbjct: 365 FELENLSAIGIERYREELPVYETTRLDNMNRVIA 352

BLAST of MC09g0195 vs. ExPASy Swiss-Prot
Match: Q9M2B3 (Protein BIG GRAIN 1-like C OS=Arabidopsis thaliana OX=3702 GN=At3g42800 PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.1e-25
Identity = 127/369 (34.42%), Postives = 180/369 (48.78%), Query Frame = 0

Query: 17  RRQNPSFSSSLLDAIYRSIDETNGVQGE------EQVVFSMAD-----FDRKARNVRDRD 76
           R++ PSFSSS+LD++YRSIDE++G+Q +      E V  S +       D K   +R   
Sbjct: 6   RKRTPSFSSSVLDSVYRSIDESDGLQSDLKGSINENVSSSSSSPSPNKKDDKLTTLRRAI 65

Query: 77  RDRNRGFKKLMNSNSSSSSSESSCGGGFSSSESESRSSSYTMLRPRPIRTEKPQFDGHGD 136
            D     +  + + SS++++ SS    FSSSE+ES  +       R +R    Q    GD
Sbjct: 66  MDE----EHWLYARSSTTTTNSSDSSSFSSSEAESYRTK------RRLRKLAEQGKRSGD 125

Query: 137 AIKVSVPNQKPKHENGFVKT----KSKALKIYGDLKKVKQPISPGGRFATFLNSLFNGGS 196
             + +           F K+    K KA+KI  +LK+ KQP+SPG R  +FLNS+F   +
Sbjct: 126 ERQRTKRTVMDNDSRLFSKSDDDKKPKAVKIIEELKRSKQPVSPGARLTSFLNSIFQSNA 185

Query: 197 PKKQKFTNSAQQNDACMRKSKSAQGSTCSSASSFSRSCLSKTPSSGEKLINN-GSTKRSV 256
            KK K                 + G T    SS S+SC S+T +  +   NN    +RS+
Sbjct: 186 -KKVKL---------------CSVGKTTDVKSSSSKSCFSRTRNKTDNNNNNCKKLERSI 245

Query: 257 RFCPVSVILDEDSRPCGHKSLQDELGLMAAATTLRNALRNDHCRADEATEDLMNSYQKKI 316
           RF PV V +D D R    K +   +       T + +++ +    D  TE    +    +
Sbjct: 246 RFYPVRVTIDGDCRDYAQKHI-TRVRKPIPEFTAKKSVKEEIKTNDHHTEFTCITRNIGL 305

Query: 317 NGDVYINREEDDEDEEEDDEEEEDDDDAVSCSSSDLFELDNLSSIGIERYRQELPVYETT 370
              V  N+ E  E+EE          DA S SSSDLFELD+   IG+ RY +ELPVYETT
Sbjct: 306 KDFVRSNKYEGKEEEE----------DAWSHSSSDLFELDSY-RIGMGRYLKELPVYETT 336

BLAST of MC09g0195 vs. ExPASy Swiss-Prot
Match: Q9LYH0 (Protein BIG GRAIN 1-like D OS=Arabidopsis thaliana OX=3702 GN=At5g12050 PE=2 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 2.1e-24
Identity = 138/374 (36.90%), Postives = 187/374 (50.00%), Query Frame = 0

Query: 18  RQNPSFSSSLLDAIYRSIDETNGVQGEEQVVFSMADFDRK----ARNVRDRDRDRNRGFK 77
           ++NPSFSS+LLD IY SID     Q  +  V S+    +K     R+V DR   R+R F 
Sbjct: 5   KRNPSFSSTLLDEIYNSIDPK--TQKTQPYVGSVNTTTKKQSIVTRSVPDRKIHRDRFF- 64

Query: 78  KLMNSNSSSSSSESSCGGGFSSSESE---------SRSSSYTMLRPRPIRTEKPQFDGHG 137
               S SSSS S SS    FSSS++E         SR   +   + +P +TE        
Sbjct: 65  ---GSVSSSSDSNSSI---FSSSDTELTHGKKTTSSRPLCFGPSKTKPRKTEDKTLFHQN 124

Query: 138 DAIKV--------SVPNQKPKHENGFVKTKSKALKIYGDLKKVKQPISPGGRFATFLNSL 197
            A +V         VP      EN       +++K  G+ KK K P SPGGR   FLNSL
Sbjct: 125 RATRVYDDYDYASDVPKFNRHDENWENTRNRRSVKSSGNQKKPKTPASPGGRIVNFLNSL 184

Query: 198 FNGGSPK----KQKFTNSAQQNDACMRKSKS---AQGSTCSSASSFSRSCLSKTPSSGEK 257
           F+  S +    K     ++  + A +RK+ +   +  +TCSSASSFSRSC++K     EK
Sbjct: 185 FSNNSKQSNAVKSYPRKTSYDDSAYVRKTSNDYHSSTTTCSSASSFSRSCMNK---GYEK 244

Query: 258 LINNGSTKRSVRFCPVSVILDEDSRPCGHKSLQDELGLMAAATTLRNALRNDHCRADE-- 317
             ++G  KRSVRF PV+VI+ E          +D      A  +++  + +   R+ E  
Sbjct: 245 --SSGRIKRSVRFSPVNVIVPESFT----SKEEDYFSNGNARKSVKKNVEDGGRRSVEEI 304

Query: 318 ATEDLMNSYQKKINGDVYINREEDDEDEEEDDEEEEDDDDAVSCSSSDLFELDNLSSIGI 361
           A E L + ++   N  V  N  ED ED++EDD    DDDD  S SSSDLFELD + +   
Sbjct: 305 AREFLRDYHKNHENSLVKTNGFEDYEDDDEDD----DDDDVASDSSSDLFELDLVGNHHH 356

BLAST of MC09g0195 vs. ExPASy Swiss-Prot
Match: Q10R09 (Protein BIG GRAIN 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BG1 PE=1 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 9.2e-20
Identity = 120/363 (33.06%), Postives = 161/363 (44.35%), Query Frame = 0

Query: 15  RHRRQNPSFSSSLLDAIYRSIDET----NGVQGEEQVVFSMADFDRKARNVRDRDRDRNR 74
           R+    PSFSS+LLDAIY+S+DE      G  G E V  +       A +  +  +    
Sbjct: 16  RYVADQPSFSSTLLDAIYKSMDEQPGHGGGATGVEAVAAAAKKQHEAALHYGNYYKPSLA 75

Query: 75  GFKKLMNSN---SSSSSSESSCGGGFSSSESESRSSSYTMLRPRPIRTEKPQFDGHGDAI 134
           G  +        ++SSSSE S  GGFSSSE+ES        R RPIRT  P         
Sbjct: 76  GSYRARAPGPHATTSSSSECSSYGGFSSSEAESSHHR----RLRPIRTTVPG-------- 135

Query: 135 KVSVPNQKPKHENGFVKTKSKALKIYGDLKKVKQPISPGGRFATFLNSLFNGGSPKKQKF 194
               P   P+      K K     I   L+ +++P SPG R A FLNS+F G        
Sbjct: 136 --GAPGPAPEK-----KAKKPGASIRAKLRDLRKPASPGARLAGFLNSIFAG-------- 195

Query: 195 TNSAQQNDACMRKSKSAQGSTCSSASSFSRSCLSKTPSSGEKLINNGSTKRSVRFCPVSV 254
               ++  A    + +   S CS+ASS+SRSCLSKTPS+       G  KR+VRF     
Sbjct: 196 ----KRAPATPPSATAGAESACSTASSYSRSCLSKTPST------RGQAKRTVRF----- 255

Query: 255 ILDEDSRPCGHKSLQDELGLMAAATTLRNALRNDHCRADEATEDLMNSYQKKINGDVYIN 314
            LD D+      ++ D   +                   EA + ++            + 
Sbjct: 256 -LDSDTESLASSTVVDRRRVPV-----------------EAVQQML------------LQ 297

Query: 315 REEDDEDEEEDDEEEEDDDDAVSCSSSDLFELDNLSSIGI--ERYRQELPVYETTHFSTN 369
           R E + DE         DDD  S +SSDLFEL+N ++I      YR ELPVYETT  + N
Sbjct: 316 RMEMESDE---------DDDESSDASSDLFELENFAAIAPAGAAYRDELPVYETTRVALN 297

BLAST of MC09g0195 vs. NCBI nr
Match: XP_022145624.1 (protein BIG GRAIN 1-like B [Momordica charantia])

HSP 1 Score: 682 bits (1760), Expect = 1.09e-245
Identity = 368/416 (88.46%), Postives = 368/416 (88.46%), Query Frame = 0

Query: 1   MDVWDQQMPEDRHGRHRRQNPSFSSSLLDAIYRSIDETNGVQGEEQVVF----------- 60
           MDVWDQQMPEDRHGRHRRQNPSFSSSLLDAIYRSIDETNGVQGEEQVV            
Sbjct: 1   MDVWDQQMPEDRHGRHRRQNPSFSSSLLDAIYRSIDETNGVQGEEQVVLYRETMRKKKQS 60

Query: 61  ------------------------------------SMADFDRKARNVRDRDRDRNRGFK 120
                                               SMADFDRKARNVRDRDRDRNRGFK
Sbjct: 61  IGLEDGEDDGLNFRRACMIEKWMEKKVSEKVVVRRSSMADFDRKARNVRDRDRDRNRGFK 120

Query: 121 KLMNSNSSSSSSESSCGGGFSSSESESRSSSYTMLRPRPIRTEKPQFDGHGDAIKVSVPN 180
           KLMNSNSSSSSSESSCGGGFSSSESESRSSSYTMLRPRPIRTEKPQFDGHGDAIKVSVPN
Sbjct: 121 KLMNSNSSSSSSESSCGGGFSSSESESRSSSYTMLRPRPIRTEKPQFDGHGDAIKVSVPN 180

Query: 181 QKPKHENGFVKTKSKALKIYGDLKKVKQPISPGGRFATFLNSLFNGGSPKKQKFTNSAQQ 240
           QKPKHENGFVKTKSKALKIYGDLKKVKQPISPGGRFATFLNSLFNGGSPKKQKFTNSAQQ
Sbjct: 181 QKPKHENGFVKTKSKALKIYGDLKKVKQPISPGGRFATFLNSLFNGGSPKKQKFTNSAQQ 240

Query: 241 NDACMRKSKSAQGSTCSSASSFSRSCLSKTPSSGEKLINNGSTKRSVRFCPVSVILDEDS 300
           NDACMRKSKSAQGSTCSSASSFSRSCLSKTPSSGEKLINNGSTKRSVRFCPVSVILDEDS
Sbjct: 241 NDACMRKSKSAQGSTCSSASSFSRSCLSKTPSSGEKLINNGSTKRSVRFCPVSVILDEDS 300

Query: 301 RPCGHKSLQDELGLMAAATTLRNALRNDHCRADEATEDLMNSYQKKINGDVYINREEDDE 360
           RPCGHKSLQDELGLMAAATTLRNALRNDHCRADEATEDLMNSYQKKINGDVYINREEDDE
Sbjct: 301 RPCGHKSLQDELGLMAAATTLRNALRNDHCRADEATEDLMNSYQKKINGDVYINREEDDE 360

Query: 361 DEEEDDEEEEDDDDAVSCSSSDLFELDNLSSIGIERYRQELPVYETTHFSTNQAIA 369
           DEEEDDEEEEDDDDAVSCSSSDLFELDNLSSIGIERYRQELPVYETTHFSTNQAIA
Sbjct: 361 DEEEDDEEEEDDDDAVSCSSSDLFELDNLSSIGIERYRQELPVYETTHFSTNQAIA 416

BLAST of MC09g0195 vs. NCBI nr
Match: XP_038896283.1 (protein BIG GRAIN 1-like B [Benincasa hispida])

HSP 1 Score: 577 bits (1487), Expect = 3.22e-204
Identity = 321/419 (76.61%), Postives = 346/419 (82.58%), Query Frame = 0

Query: 1   MDVWDQQMPEDRHG--RHRRQNPSFSSSLLDAIYRSIDETNGVQGEEQVVF--------- 60
           MD+WD+Q+PE  H   RHRRQNPSFSSSLLDAIYRSIDE N VQ EEQV+          
Sbjct: 1   MDLWDKQLPEHPHAGARHRRQNPSFSSSLLDAIYRSIDEPNAVQREEQVILYTETMRKKQ 60

Query: 61  ------------------------------------SMADFDRKARNVRDRDRDRNRGFK 120
                                               SMADFDRKARNVRDRD      FK
Sbjct: 61  SIDDEIEDDRLNFRRACMIEKWMEKKVSEKVVVRRTSMADFDRKARNVRDRD------FK 120

Query: 121 KLMNSNSSSSSSESSCGGGFSSSESESRSSSYTMLRPRPIRTEKPQFDGHGDAIKVSVPN 180
           KLMNS  SSSSS+SS GGGFSSSESE+RSSSYTM RPRPIRTEKP F+G+GD IKVS+PN
Sbjct: 121 KLMNS--SSSSSDSSSGGGFSSSESETRSSSYTMQRPRPIRTEKPHFEGYGDGIKVSIPN 180

Query: 181 QKPKHENGFVKTKSKALKIYGDLKKVKQPISPGGRFATFLNSLFNGGSPKKQKFTNSAQQ 240
           QKPKHE+ FVKTKSKALKIYGDLKKVKQPISPGGR ATFLNSLFNGGSPKKQKFTNS +Q
Sbjct: 181 QKPKHESSFVKTKSKALKIYGDLKKVKQPISPGGRLATFLNSLFNGGSPKKQKFTNSVKQ 240

Query: 241 NDACMRKSKSAQGSTCSSASSFSRSCLSKTPSSGEKLINNGSTKRSVRFCPVSVILDEDS 300
           NDACMRKSKSAQGSTCSSASSFSRSCLSKTPSSGEKLIN+GSTKRSVRFCPVSVILDEDS
Sbjct: 241 NDACMRKSKSAQGSTCSSASSFSRSCLSKTPSSGEKLINSGSTKRSVRFCPVSVILDEDS 300

Query: 301 RPCGHKSLQDELGLMAAATTLRNALRNDHCRADEATEDLM---NSYQKKINGDVYINREE 360
           RPCGHKSLQDELGLMAAATTLRNALRNDHCRA+EA+ED+M   NSYQKKINGDVYINREE
Sbjct: 301 RPCGHKSLQDELGLMAAATTLRNALRNDHCRAEEASEDVMMNMNSYQKKINGDVYINREE 360

Query: 361 DDEDEEEDDEEEEDDDDAVSCSSSDLFELDNLSSIGIERYRQELPVYETTHFSTNQAIA 369
           D EDE+E++E+++DD+DA+SCSSSDLFELDNLSSIGIERYR+ELPVYETTHF+TNQAIA
Sbjct: 361 D-EDEDEEEEDDDDDEDALSCSSSDLFELDNLSSIGIERYREELPVYETTHFNTNQAIA 410

BLAST of MC09g0195 vs. NCBI nr
Match: KAG6593542.1 (Protein BIG GRAIN 1-like A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 568 bits (1465), Expect = 1.07e-200
Identity = 322/428 (75.23%), Postives = 346/428 (80.84%), Query Frame = 0

Query: 1   MDVWDQQM--PEDRHGRHRRQNPSFSSSLLDAIYRSIDETNGVQGEEQVVF--------- 60
           MDVWDQQ+  P+DRHGRHRRQNPSFSSSLLDAIYRSIDE+NGV+GEE+V+          
Sbjct: 1   MDVWDQQLQLPQDRHGRHRRQNPSFSSSLLDAIYRSIDESNGVEGEEKVILYRETMRKKQ 60

Query: 61  -------------------------------------SMADFDRKARNVRDRD----RDR 120
                                                SMADFDRKARNVRDRD    RDR
Sbjct: 61  SIAMKEDEDDRLNFRRACMIEKWMEKKVSEKVVVRKSSMADFDRKARNVRDRDNRDNRDR 120

Query: 121 ----NRGFKKLMNSNSSSSSSESSCGGGFSSSESESRSSSYTMLRPRPIRTEKPQFDGHG 180
               +R FKKLMNS  SSSSSESS GGGFSSSESESRSSSYTM RPRPIRTEK +FDGH 
Sbjct: 121 RDHRDRDFKKLMNS--SSSSSESSSGGGFSSSESESRSSSYTMHRPRPIRTEKAEFDGHS 180

Query: 181 DAIKVSVPNQKPKHENGFVKTKSKALKIYGDLKKVKQPISPGGRFATFLNSLFNGGSPKK 240
           D IK S+PNQKPKHENGFVKTKSKAL+IYGDLKKVKQPISPGGR ATFLNSLFNGGSP+K
Sbjct: 181 DEIKFSIPNQKPKHENGFVKTKSKALRIYGDLKKVKQPISPGGRLATFLNSLFNGGSPRK 240

Query: 241 QKFTNSAQQNDACMRKSKSAQGSTCSSASSFSRSCLSKTPSSGEKLINNGSTKRSVRFCP 300
           QKFTNS++Q DACMRKSKS QGSTCSSASSFSRSCLSKTPS+GEKLINNGSTKRSVRFC 
Sbjct: 241 QKFTNSSKQTDACMRKSKSTQGSTCSSASSFSRSCLSKTPSNGEKLINNGSTKRSVRFC- 300

Query: 301 VSVILDEDSRPCGHKSLQDELGLMAAATTLRNALRNDHCRADEATEDLMNSYQKKINGDV 360
               LDEDSRPCGHKSLQD+LGLMAAATT+RN LRNDHC  +EA+ DLMNSYQKKINGDV
Sbjct: 301 ----LDEDSRPCGHKSLQDKLGLMAAATTMRNVLRNDHCLEEEASADLMNSYQKKINGDV 360

Query: 361 YINR--EEDDEDEEEDDE-EEEDDDDAVSCSSSDLFELDNLSSIGIERYRQELPVYETTH 369
           YINR  E +DEDEE+DDE EEEDD+DAVSCSSSDLFELDNLSSIGI+RYR+ELPVYETTH
Sbjct: 361 YINRVVENEDEDEEDDDEDEEEDDEDAVSCSSSDLFELDNLSSIGIQRYREELPVYETTH 420

BLAST of MC09g0195 vs. NCBI nr
Match: KAG7025884.1 (Protein BIG GRAIN 1-like A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 568 bits (1465), Expect = 1.07e-200
Identity = 322/428 (75.23%), Postives = 346/428 (80.84%), Query Frame = 0

Query: 1   MDVWDQQM--PEDRHGRHRRQNPSFSSSLLDAIYRSIDETNGVQGEEQVVF--------- 60
           MDVWDQQ+  P+DRHGRHRRQNPSFSSSLLDAIYRSIDE+NGV+GEE+V+          
Sbjct: 1   MDVWDQQLQLPQDRHGRHRRQNPSFSSSLLDAIYRSIDESNGVEGEEKVILYRETMRKKQ 60

Query: 61  -------------------------------------SMADFDRKARNVRDRD----RDR 120
                                                SMADFDRKARNVRDRD    RDR
Sbjct: 61  SVAMKEDEDDRLNFRRACMIEKWMEKKVSEKVVVRKSSMADFDRKARNVRDRDNRDNRDR 120

Query: 121 ----NRGFKKLMNSNSSSSSSESSCGGGFSSSESESRSSSYTMLRPRPIRTEKPQFDGHG 180
               +R FKKLMNS  SSSSSESS GGGFSSSESESRSSSYTM RPRPIRTEK +FDGH 
Sbjct: 121 RDHRDRDFKKLMNS--SSSSSESSSGGGFSSSESESRSSSYTMHRPRPIRTEKAEFDGHS 180

Query: 181 DAIKVSVPNQKPKHENGFVKTKSKALKIYGDLKKVKQPISPGGRFATFLNSLFNGGSPKK 240
           D IK S+PNQKPKHENGFVKTKSKAL+IYGDLKKVKQPISPGGR ATFLNSLFNGGSP+K
Sbjct: 181 DEIKFSIPNQKPKHENGFVKTKSKALRIYGDLKKVKQPISPGGRLATFLNSLFNGGSPRK 240

Query: 241 QKFTNSAQQNDACMRKSKSAQGSTCSSASSFSRSCLSKTPSSGEKLINNGSTKRSVRFCP 300
           QKFTNS++Q DACMRKSKS QGSTCSSASSFSRSCLSKTPS+GEKLINNGSTKRSVRFC 
Sbjct: 241 QKFTNSSKQTDACMRKSKSTQGSTCSSASSFSRSCLSKTPSNGEKLINNGSTKRSVRFC- 300

Query: 301 VSVILDEDSRPCGHKSLQDELGLMAAATTLRNALRNDHCRADEATEDLMNSYQKKINGDV 360
               LDEDSRPCGHKSLQD+LGLMAAATT+RN LRNDHC  +EA+ DLMNSYQKKINGDV
Sbjct: 301 ----LDEDSRPCGHKSLQDKLGLMAAATTMRNVLRNDHCLEEEASADLMNSYQKKINGDV 360

Query: 361 YINR--EEDDEDEEEDDEEEEDDD-DAVSCSSSDLFELDNLSSIGIERYRQELPVYETTH 369
           YINR  E +DEDEE+DDEEEE+DD DAVSCSSSDLFELDNLSSIGI+RYR+ELPVYETTH
Sbjct: 361 YINRVVENEDEDEEDDDEEEEEDDEDAVSCSSSDLFELDNLSSIGIQRYREELPVYETTH 420

BLAST of MC09g0195 vs. NCBI nr
Match: XP_022964194.1 (protein BIG GRAIN 1-like B [Cucurbita moschata])

HSP 1 Score: 563 bits (1451), Expect = 1.29e-198
Identity = 319/425 (75.06%), Postives = 344/425 (80.94%), Query Frame = 0

Query: 1   MDVWDQQM--PEDRHGRHRRQNPSFSSSLLDAIYRSIDETNGVQGEEQVVF--------- 60
           MDVWDQQ+  P+DRHGRHRRQNPSFSSSLLDAIYRSIDE+NGV+GEE+V+          
Sbjct: 1   MDVWDQQLQLPQDRHGRHRRQNPSFSSSLLDAIYRSIDESNGVEGEEKVILYRETMRKKQ 60

Query: 61  -------------------------------------SMADFDRKARNVRDRD-RDR--- 120
                                                SMADFDRKARNVRDRD RDR   
Sbjct: 61  SIAMKEDEDDRLNFRRACMIEKWMEKKVSEKVVVRKSSMADFDRKARNVRDRDNRDRRDH 120

Query: 121 -NRGFKKLMNSNSSSSSSESSCGGGFSSSESESRSSSYTMLRPRPIRTEKPQFDGHGDAI 180
            +R FKKLMNS  SSSSSESS GGGFSSSESESRSSSYTM RPRPIRTEK +FDGH D I
Sbjct: 121 RDRDFKKLMNS--SSSSSESSSGGGFSSSESESRSSSYTMHRPRPIRTEKAEFDGHSDEI 180

Query: 181 KVSVPNQKPKHENGFVKTKSKALKIYGDLKKVKQPISPGGRFATFLNSLFNGGSPKKQKF 240
           K S+PNQKPKHENGFVKTKSKAL+IYGDLKKVKQPISPGGR ATFLNSLFNGGSP+KQKF
Sbjct: 181 KFSIPNQKPKHENGFVKTKSKALRIYGDLKKVKQPISPGGRLATFLNSLFNGGSPRKQKF 240

Query: 241 TNSAQQNDACMRKSKSAQGSTCSSASSFSRSCLSKTPSSGEKLINNGSTKRSVRFCPVSV 300
           TNS++Q DACMRKSKS QGSTCSSASSFSRSCLSKT S+GEKLINNGSTKRSVRFC    
Sbjct: 241 TNSSKQTDACMRKSKSTQGSTCSSASSFSRSCLSKTSSNGEKLINNGSTKRSVRFC---- 300

Query: 301 ILDEDSRPCGHKSLQDELGLMAAATTLRNALRNDHCRADEATEDLMNSYQKKINGDVYIN 360
            LDEDSRPCGHK LQ++LGLMAAATT+RN LRNDHC  +EA+ DLMNSYQKKINGDVYIN
Sbjct: 301 -LDEDSRPCGHKGLQNKLGLMAAATTMRNVLRNDHCLEEEASADLMNSYQKKINGDVYIN 360

Query: 361 R--EEDDEDEEEDDEEEEDDD-DAVSCSSSDLFELDNLSSIGIERYRQELPVYETTHFST 369
           R  E +DEDEE+DDEEEE+DD DAVSCSSSDLFELDNLSSIGI+RYR+ELPVYETTHF+T
Sbjct: 361 RVVENEDEDEEDDDEEEEEDDEDAVSCSSSDLFELDNLSSIGIQRYREELPVYETTHFTT 418

BLAST of MC09g0195 vs. ExPASy TrEMBL
Match: A0A6J1CVT3 (protein BIG GRAIN 1-like B OS=Momordica charantia OX=3673 GN=LOC111015022 PE=3 SV=1)

HSP 1 Score: 682 bits (1760), Expect = 5.30e-246
Identity = 368/416 (88.46%), Postives = 368/416 (88.46%), Query Frame = 0

Query: 1   MDVWDQQMPEDRHGRHRRQNPSFSSSLLDAIYRSIDETNGVQGEEQVVF----------- 60
           MDVWDQQMPEDRHGRHRRQNPSFSSSLLDAIYRSIDETNGVQGEEQVV            
Sbjct: 1   MDVWDQQMPEDRHGRHRRQNPSFSSSLLDAIYRSIDETNGVQGEEQVVLYRETMRKKKQS 60

Query: 61  ------------------------------------SMADFDRKARNVRDRDRDRNRGFK 120
                                               SMADFDRKARNVRDRDRDRNRGFK
Sbjct: 61  IGLEDGEDDGLNFRRACMIEKWMEKKVSEKVVVRRSSMADFDRKARNVRDRDRDRNRGFK 120

Query: 121 KLMNSNSSSSSSESSCGGGFSSSESESRSSSYTMLRPRPIRTEKPQFDGHGDAIKVSVPN 180
           KLMNSNSSSSSSESSCGGGFSSSESESRSSSYTMLRPRPIRTEKPQFDGHGDAIKVSVPN
Sbjct: 121 KLMNSNSSSSSSESSCGGGFSSSESESRSSSYTMLRPRPIRTEKPQFDGHGDAIKVSVPN 180

Query: 181 QKPKHENGFVKTKSKALKIYGDLKKVKQPISPGGRFATFLNSLFNGGSPKKQKFTNSAQQ 240
           QKPKHENGFVKTKSKALKIYGDLKKVKQPISPGGRFATFLNSLFNGGSPKKQKFTNSAQQ
Sbjct: 181 QKPKHENGFVKTKSKALKIYGDLKKVKQPISPGGRFATFLNSLFNGGSPKKQKFTNSAQQ 240

Query: 241 NDACMRKSKSAQGSTCSSASSFSRSCLSKTPSSGEKLINNGSTKRSVRFCPVSVILDEDS 300
           NDACMRKSKSAQGSTCSSASSFSRSCLSKTPSSGEKLINNGSTKRSVRFCPVSVILDEDS
Sbjct: 241 NDACMRKSKSAQGSTCSSASSFSRSCLSKTPSSGEKLINNGSTKRSVRFCPVSVILDEDS 300

Query: 301 RPCGHKSLQDELGLMAAATTLRNALRNDHCRADEATEDLMNSYQKKINGDVYINREEDDE 360
           RPCGHKSLQDELGLMAAATTLRNALRNDHCRADEATEDLMNSYQKKINGDVYINREEDDE
Sbjct: 301 RPCGHKSLQDELGLMAAATTLRNALRNDHCRADEATEDLMNSYQKKINGDVYINREEDDE 360

Query: 361 DEEEDDEEEEDDDDAVSCSSSDLFELDNLSSIGIERYRQELPVYETTHFSTNQAIA 369
           DEEEDDEEEEDDDDAVSCSSSDLFELDNLSSIGIERYRQELPVYETTHFSTNQAIA
Sbjct: 361 DEEEDDEEEEDDDDAVSCSSSDLFELDNLSSIGIERYRQELPVYETTHFSTNQAIA 416

BLAST of MC09g0195 vs. ExPASy TrEMBL
Match: A0A6J1HME7 (protein BIG GRAIN 1-like B OS=Cucurbita moschata OX=3662 GN=LOC111464284 PE=3 SV=1)

HSP 1 Score: 563 bits (1451), Expect = 6.25e-199
Identity = 319/425 (75.06%), Postives = 344/425 (80.94%), Query Frame = 0

Query: 1   MDVWDQQM--PEDRHGRHRRQNPSFSSSLLDAIYRSIDETNGVQGEEQVVF--------- 60
           MDVWDQQ+  P+DRHGRHRRQNPSFSSSLLDAIYRSIDE+NGV+GEE+V+          
Sbjct: 1   MDVWDQQLQLPQDRHGRHRRQNPSFSSSLLDAIYRSIDESNGVEGEEKVILYRETMRKKQ 60

Query: 61  -------------------------------------SMADFDRKARNVRDRD-RDR--- 120
                                                SMADFDRKARNVRDRD RDR   
Sbjct: 61  SIAMKEDEDDRLNFRRACMIEKWMEKKVSEKVVVRKSSMADFDRKARNVRDRDNRDRRDH 120

Query: 121 -NRGFKKLMNSNSSSSSSESSCGGGFSSSESESRSSSYTMLRPRPIRTEKPQFDGHGDAI 180
            +R FKKLMNS  SSSSSESS GGGFSSSESESRSSSYTM RPRPIRTEK +FDGH D I
Sbjct: 121 RDRDFKKLMNS--SSSSSESSSGGGFSSSESESRSSSYTMHRPRPIRTEKAEFDGHSDEI 180

Query: 181 KVSVPNQKPKHENGFVKTKSKALKIYGDLKKVKQPISPGGRFATFLNSLFNGGSPKKQKF 240
           K S+PNQKPKHENGFVKTKSKAL+IYGDLKKVKQPISPGGR ATFLNSLFNGGSP+KQKF
Sbjct: 181 KFSIPNQKPKHENGFVKTKSKALRIYGDLKKVKQPISPGGRLATFLNSLFNGGSPRKQKF 240

Query: 241 TNSAQQNDACMRKSKSAQGSTCSSASSFSRSCLSKTPSSGEKLINNGSTKRSVRFCPVSV 300
           TNS++Q DACMRKSKS QGSTCSSASSFSRSCLSKT S+GEKLINNGSTKRSVRFC    
Sbjct: 241 TNSSKQTDACMRKSKSTQGSTCSSASSFSRSCLSKTSSNGEKLINNGSTKRSVRFC---- 300

Query: 301 ILDEDSRPCGHKSLQDELGLMAAATTLRNALRNDHCRADEATEDLMNSYQKKINGDVYIN 360
            LDEDSRPCGHK LQ++LGLMAAATT+RN LRNDHC  +EA+ DLMNSYQKKINGDVYIN
Sbjct: 301 -LDEDSRPCGHKGLQNKLGLMAAATTMRNVLRNDHCLEEEASADLMNSYQKKINGDVYIN 360

Query: 361 R--EEDDEDEEEDDEEEEDDD-DAVSCSSSDLFELDNLSSIGIERYRQELPVYETTHFST 369
           R  E +DEDEE+DDEEEE+DD DAVSCSSSDLFELDNLSSIGI+RYR+ELPVYETTHF+T
Sbjct: 361 RVVENEDEDEEDDDEEEEEDDEDAVSCSSSDLFELDNLSSIGIQRYREELPVYETTHFTT 418

BLAST of MC09g0195 vs. ExPASy TrEMBL
Match: A0A0A0K9K7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G450580 PE=3 SV=1)

HSP 1 Score: 562 bits (1448), Expect = 2.22e-198
Identity = 318/431 (73.78%), Postives = 344/431 (79.81%), Query Frame = 0

Query: 1   MDVWDQQMPEDRHG-RHRRQNPSFSSSLLDAIYRSIDETNGVQGEEQVVF---------- 60
           MD+WD+Q+PE  HG RHR+QNPSFSSSLLDAIYRSID+T GVQ +E ++F          
Sbjct: 1   MDLWDKQLPEHPHGPRHRKQNPSFSSSLLDAIYRSIDDTTGVQRQEHLIFYTAETTRNKQ 60

Query: 61  --------------------------------------------SMADFDRKARNVRDRD 120
                                                       SMADFDRKARNVRDRD
Sbjct: 61  SSSSSSSSIDHHEIQEDRLNFRRACMIEKWMEKKVCEKVVVRRTSMADFDRKARNVRDRD 120

Query: 121 RDRNRGFKKLMNSNSSSSSSESSCGGGFSSSESESRSSSYTMLRPRPIRTEKPQFDGHGD 180
            +      KLMNS  SSSSSESS GGGFSSSESE+RSSSYTM RPRPIRTEKPQF+G+GD
Sbjct: 121 FNH-----KLMNS--SSSSSESSSGGGFSSSESETRSSSYTMQRPRPIRTEKPQFEGYGD 180

Query: 181 AIKVSVPNQKPKHENGFVKTKSKALKIYGDLKKVKQPISPGGRFATFLNSLFNGGSPKKQ 240
            IKVS+PNQKPKHEN FVKTKSKALKIYGDLKKVKQPISPGGR ATFLNSLFNGGSPKKQ
Sbjct: 181 GIKVSIPNQKPKHENSFVKTKSKALKIYGDLKKVKQPISPGGRLATFLNSLFNGGSPKKQ 240

Query: 241 KFTNSAQQNDACMRKSKSAQGSTCSSASSFSRSCLSKTPSSGEKLINNGSTKRSVRFCPV 300
           KFTNS++QNDACMRKSKSAQGSTCSSASSFSRSCLSKTPSSGEKLIN+GSTKRSVRFCPV
Sbjct: 241 KFTNSSKQNDACMRKSKSAQGSTCSSASSFSRSCLSKTPSSGEKLINSGSTKRSVRFCPV 300

Query: 301 SVILDEDSRPCGHKSLQDELGLMAAATTLRNALRNDHCRADEATED-----LMNSYQKKI 360
           SVILDE+SRPCGHKSLQDELGLMAA TTLRNALRNDHCR  E +E+     + N Y+KKI
Sbjct: 301 SVILDEESRPCGHKSLQDELGLMAATTTLRNALRNDHCRGKEGSEEQVLMSMNNCYEKKI 360

Query: 361 -NGDVYINREED-DEDEEEDDEEEEDDDDAVSCSSSDLFELDNLSSIGIERYRQELPVYE 369
            NGDVYINREED DEDEEED+EE+ED+DDAVSCSSSDLFELDNLSSIGIERYR+ELPVYE
Sbjct: 361 INGDVYINREEDEDEDEEEDEEEDEDEDDAVSCSSSDLFELDNLSSIGIERYREELPVYE 420

BLAST of MC09g0195 vs. ExPASy TrEMBL
Match: A0A6J1KKD7 (protein BIG GRAIN 1-like B OS=Cucurbita maxima OX=3661 GN=LOC111494822 PE=3 SV=1)

HSP 1 Score: 555 bits (1430), Expect = 1.09e-195
Identity = 316/428 (73.83%), Postives = 342/428 (79.91%), Query Frame = 0

Query: 1   MDVWDQQM--PEDRHGRHRRQNPSFSSSLLDAIYRSIDETNGVQGEEQVVF--------- 60
           MDVWDQQ+  P+DRHGRHRRQNPSFSSSLLDAIYRSIDE+NGV+GEE+V+          
Sbjct: 1   MDVWDQQLQLPQDRHGRHRRQNPSFSSSLLDAIYRSIDESNGVEGEEKVILYRETMRKKQ 60

Query: 61  -------------------------------------SMADFDRKARNVRDRD----RDR 120
                                                SM DFDRKARNVRDRD    RDR
Sbjct: 61  SIAMKEDEDDRLNFRRACMIEKWMEKKVSEKVVVRKSSMVDFDRKARNVRDRDNRDNRDR 120

Query: 121 ----NRGFKKLMNSNSSSSSSESSCGGGFSSSESESRSSSYTMLRPRPIRTEKPQFDGHG 180
               +R FKKLMNS  SSSSSESS GGGFSSSESESRSSSYTM RPRPIR EK + DGH 
Sbjct: 121 RDHRDRDFKKLMNS--SSSSSESSSGGGFSSSESESRSSSYTMHRPRPIRMEKAELDGHS 180

Query: 181 DAIKVSVPNQKPKHENGFVKTKSKALKIYGDLKKVKQPISPGGRFATFLNSLFNGGSPKK 240
             IK S+PNQKPKHENGFVKTKSKAL+IYGDLKKVKQPISPGGR ATFLNSLFNGGSP+K
Sbjct: 181 VEIKFSIPNQKPKHENGFVKTKSKALRIYGDLKKVKQPISPGGRLATFLNSLFNGGSPRK 240

Query: 241 QKFTNSAQQNDACMRKSKSAQGSTCSSASSFSRSCLSKTPSSGEKLINNGSTKRSVRFCP 300
           QKFTNS++Q DACMRKSKS QGSTCSSASSFSRSCLSKTPS+GEKLINNGSTKRSVRFC 
Sbjct: 241 QKFTNSSKQTDACMRKSKSTQGSTCSSASSFSRSCLSKTPSNGEKLINNGSTKRSVRFC- 300

Query: 301 VSVILDEDSRPCGHKSLQDELGLMAAATTLRNALRNDHCRADEATEDLMNSYQKKINGDV 360
               LDEDSRPCGHKSLQ++LGLMAAATT+RN LRNDHC  +EA+ DLMNSYQKKIN DV
Sbjct: 301 ----LDEDSRPCGHKSLQNKLGLMAAATTMRNVLRNDHCLEEEASADLMNSYQKKINDDV 360

Query: 361 YINR--EEDDEDEEEDDE-EEEDDDDAVSCSSSDLFELDNLSSIGIERYRQELPVYETTH 369
           YINR  E++DEDEE+DDE EEEDD+DAVSCSSSDLFELDNLSSIGI+RYR+ELPVYETTH
Sbjct: 361 YINRVVEDEDEDEEDDDEDEEEDDEDAVSCSSSDLFELDNLSSIGIQRYREELPVYETTH 420

BLAST of MC09g0195 vs. ExPASy TrEMBL
Match: A0A6J1GM94 (protein BIG GRAIN 1-like B OS=Cucurbita moschata OX=3662 GN=LOC111455790 PE=3 SV=1)

HSP 1 Score: 550 bits (1418), Expect = 2.48e-194
Identity = 309/415 (74.46%), Postives = 332/415 (80.00%), Query Frame = 0

Query: 1   MDVWDQQMPEDRHGRHRRQNPSFSSSLLDAIYRSIDETNGVQGEEQVVF----------- 60
           MDVWD+Q+ EDRHGRHR+QNPSFSSSLLDAIYRSIDE+NG + EEQV+            
Sbjct: 1   MDVWDKQLQEDRHGRHRKQNPSFSSSLLDAIYRSIDESNGDEREEQVILYRETMRKKQSI 60

Query: 61  -----------------------------------SMADFDRKARNVRDRDRDRNRGFKK 120
                                              SM DFDRKARNVRDRD      FKK
Sbjct: 61  GFEEDEDDRLNFRRACMIEKWMEKKVSEKVVVRRSSMVDFDRKARNVRDRD------FKK 120

Query: 121 LMNSNSSSSSSESSCGGGFSSSESESRSSSYTMLRPRPIRTEKPQFDGHGDAIKVSVPNQ 180
           LMNS  SSSSSESS GGGFSSSESE+RSS YTM RPRPIRTEKPQF+             
Sbjct: 121 LMNS--SSSSSESSSGGGFSSSESETRSSPYTMQRPRPIRTEKPQFE------------- 180

Query: 181 KPKHENGFVKTKSKALKIYGDLKKVKQPISPGGRFATFLNSLFNGGSPKKQKFTNSAQQN 240
           KPK+ENGFVKTKSKALKIYGDLKKVKQPISPGGR ATFLNSLFNGGSPKKQKFTNSA+QN
Sbjct: 181 KPKNENGFVKTKSKALKIYGDLKKVKQPISPGGRLATFLNSLFNGGSPKKQKFTNSAKQN 240

Query: 241 DACMRKSKSAQGSTCSSASSFSRSCLSKTPSSGEKLINNGSTKRSVRFCPVSVILDEDSR 300
           DACMRKSKS+QGSTCSSASSFSRSCLSKTPSS EKLIN+ STKRSVRFCPVSVILDEDSR
Sbjct: 241 DACMRKSKSSQGSTCSSASSFSRSCLSKTPSSSEKLINSSSTKRSVRFCPVSVILDEDSR 300

Query: 301 PCGHKSLQDELGLMAAATTLRNALRNDHCRADEATEDLMNSYQKKINGDVYINREEDDED 360
           PCGHKSLQDELGLMAAATTLRNA+RNDHCRA+EA++DLMNS+QKKIN DVYINREE++ED
Sbjct: 301 PCGHKSLQDELGLMAAATTLRNAIRNDHCRAEEASKDLMNSFQKKINDDVYINREEEEED 360

Query: 361 EEEDDEEEEDDDDAVSCSSSDLFELDNLSSIGIERYRQELPVYETTHFSTNQAIA 369
              DDE+E+DD+DA SCSSSDLFELDNLSSIGIERYR+ELPVYETTHFSTNQAIA
Sbjct: 361 ---DDEDEDDDEDAASCSSSDLFELDNLSSIGIERYREELPVYETTHFSTNQAIA 391

BLAST of MC09g0195 vs. TAIR 10
Match: AT1G54200.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13980.1); Has 1084 Blast hits to 581 proteins in 136 species: Archae - 0; Bacteria - 72; Metazoa - 212; Fungi - 78; Plants - 102; Viruses - 0; Other Eukaryotes - 620 (source: NCBI BLink). )

HSP 1 Score: 240.0 bits (611), Expect = 3.1e-63
Identity = 181/384 (47.14%), Postives = 234/384 (60.94%), Query Frame = 0

Query: 1   MDVWD-QQMPEDRHGRHR----RQNPSFSSSLLDAIYRSIDETNGVQGEEQVVFSMADFD 60
           MD WD   +P D   R R     ++PSFSS+LLD IYRSID+++      +        D
Sbjct: 1   MDPWDTYNIPLDHQHRLRNRRDHRHPSFSSTLLDQIYRSIDDSSTNSSSMRKTKHQNRED 60

Query: 61  RKARNVRDRDRDRNRGFKKLMNSNSSSSSSESSCGGGFSSSESE----SRSSSYTMLRPR 120
            +    R  D +R++  K +       SSS SS   GFSSSES+       SS  +  P+
Sbjct: 61  TRVSANRRDDFNRSKNLKTIEPVFFKHSSSSSSDSSGFSSSESDYFYRRSKSSPAISHPK 120

Query: 121 PIRTEKPQFDGHGDAIKVSVPNQKPKHENG-FVKTKSKALKIYGDLKKVKQPISPGGRFA 180
           PIRT   +F+      +   PN   K E+G F+KTKSKALKIY DLKKVKQPISPGGR A
Sbjct: 121 PIRTTVERFERSPQNHR---PNSSNKQEHGSFLKTKSKALKIYSDLKKVKQPISPGGRLA 180

Query: 181 TFLNSLFNG-GSPKKQKFTNSAQQNDACMRKSKSAQGSTCSSASSFSRSCLSKTPSSGEK 240
           TFLNS+F G G+ KK    N+   +      + S+  +TCSSASSFSRSCLSKTPSS EK
Sbjct: 181 TFLNSIFTGAGNTKKLNKINTTVTSTTAAAAAASST-TTCSSASSFSRSCLSKTPSSSEK 240

Query: 241 LINNGSTKRSVRFCPVSVILDEDSRPCGHKSLQ----DELGLMAAATTLRNALRNDHCRA 300
                 +KRSVRFCPV+VI DEDS    +K+ +    +E    +   TL N +  ++ R 
Sbjct: 241 ------SKRSVRFCPVNVIFDEDSSKYNNKNNKVYGNNEREYESIRHTLENRVMEENRRV 300

Query: 301 DEATEDLMNSYQKKINGDVYINREEDDEDEEEDDEEEEDDDDAVSCSSSDLFELDNLSSI 360
            EA ++L+ SYQKK       N+E  +   E  D+EE+DDDDA+SC+SSDLFELDNLS+I
Sbjct: 301 IEAAKELLRSYQKK-------NKEVIEVSVE--DDEEDDDDDALSCTSSDLFELDNLSAI 360

Query: 361 GIERYRQELPVYETTHFSTNQAIA 370
           GI+RYR+ELPVYETT  +TN+ I+
Sbjct: 361 GIDRYREELPVYETTRLNTNRIIS 365

BLAST of MC09g0195 vs. TAIR 10
Match: AT3G13980.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G54200.1); Has 1485 Blast hits to 418 proteins in 98 species: Archae - 0; Bacteria - 6; Metazoa - 246; Fungi - 61; Plants - 107; Viruses - 6; Other Eukaryotes - 1059 (source: NCBI BLink). )

HSP 1 Score: 203.0 bits (515), Expect = 4.2e-52
Identity = 178/394 (45.18%), Postives = 222/394 (56.35%), Query Frame = 0

Query: 4   WDQQMPEDRHGRHRRQNPSFSSSLLDAIYRSIDETNGVQGEEQVVFSMADF-DRKARNVR 63
           W++      H     +NPSFSS+LLD IYRSID+++     E +         R A    
Sbjct: 5   WEKPKSSSHH-----RNPSFSSTLLDQIYRSIDDSSPPPPLESIKKKKHHHQQRNASLHE 64

Query: 64  DRD-----------RDRNRGFKK---LMNSNSSSSSSESSCGGGFSSSESE-----SRSS 123
           DR+            D  R  +K   L +SNSSSS S      GFSSSES+     S+SS
Sbjct: 65  DREISPIYHRRSIAADFERSRRKTDFLRHSNSSSSDS-----SGFSSSESDSFHGRSKSS 124

Query: 124 ----SYTMLRPRPIRTEKPQFDGHGDAIKVSVPNQKPKHENGFVKTKSKALKIYGDLKKV 183
               S +  +P+PIRT       H  A+      QKPK   GF++TKSKALKIY DLKKV
Sbjct: 125 ASPPSSSRQQPKPIRTSSVD---HSSAV------QKPKELGGFLRTKSKALKIYSDLKKV 184

Query: 184 KQPISPGGRFATFLNSLFNGG--SPKKQKFTNSAQQNDACMRKSKSAQGSTCSSASSFSR 243
           KQPISPGGR ATFLNSLF     +PKK K T +     A + +  S+  STCSSASSFSR
Sbjct: 185 KQPISPGGRLATFLNSLFTNAATNPKKHKKTTTV----AVVEEPHSS--STCSSASSFSR 244

Query: 244 SCLSKTPSSGEKLINNGSTKRSVRFCPVSVILDEDSRPCGHKSLQDELGLMAAATTLRNA 303
           SCLSKTPSS      +G +KRSVRFCPV+VILDEDS      +  +E            A
Sbjct: 245 SCLSKTPSS------SGKSKRSVRFCPVNVILDEDSSFTMPYAYNNE-----RLYDNNEA 304

Query: 304 LR-NDHCRADEATEDLMNSYQKKINGDVYINREEDDEDEEEDDEEEEDDDDAVSCSSSDL 363
            R  +H R  +A +DL+ +Y  K                  ++ EE+D+DDA SC+SSDL
Sbjct: 305 KRVEEHRRVIQAAKDLLRTYHNK----------NKVTTTNINNVEEDDEDDAASCASSDL 352

Query: 364 FELDNLSSIGIERYRQELPVYETTHF-STNQAIA 370
           FEL+NLS+IGIERYR+ELPVYETT   + N+ IA
Sbjct: 365 FELENLSAIGIERYREELPVYETTRLDNMNRVIA 352

BLAST of MC09g0195 vs. TAIR 10
Match: AT3G42800.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G54200.1); Has 853 Blast hits to 340 proteins in 98 species: Archae - 0; Bacteria - 94; Metazoa - 145; Fungi - 62; Plants - 95; Viruses - 0; Other Eukaryotes - 457 (source: NCBI BLink). )

HSP 1 Score: 119.0 bits (297), Expect = 8.0e-27
Identity = 127/369 (34.42%), Postives = 180/369 (48.78%), Query Frame = 0

Query: 17  RRQNPSFSSSLLDAIYRSIDETNGVQGE------EQVVFSMAD-----FDRKARNVRDRD 76
           R++ PSFSSS+LD++YRSIDE++G+Q +      E V  S +       D K   +R   
Sbjct: 6   RKRTPSFSSSVLDSVYRSIDESDGLQSDLKGSINENVSSSSSSPSPNKKDDKLTTLRRAI 65

Query: 77  RDRNRGFKKLMNSNSSSSSSESSCGGGFSSSESESRSSSYTMLRPRPIRTEKPQFDGHGD 136
            D     +  + + SS++++ SS    FSSSE+ES  +       R +R    Q    GD
Sbjct: 66  MDE----EHWLYARSSTTTTNSSDSSSFSSSEAESYRTK------RRLRKLAEQGKRSGD 125

Query: 137 AIKVSVPNQKPKHENGFVKT----KSKALKIYGDLKKVKQPISPGGRFATFLNSLFNGGS 196
             + +           F K+    K KA+KI  +LK+ KQP+SPG R  +FLNS+F   +
Sbjct: 126 ERQRTKRTVMDNDSRLFSKSDDDKKPKAVKIIEELKRSKQPVSPGARLTSFLNSIFQSNA 185

Query: 197 PKKQKFTNSAQQNDACMRKSKSAQGSTCSSASSFSRSCLSKTPSSGEKLINN-GSTKRSV 256
            KK K                 + G T    SS S+SC S+T +  +   NN    +RS+
Sbjct: 186 -KKVKL---------------CSVGKTTDVKSSSSKSCFSRTRNKTDNNNNNCKKLERSI 245

Query: 257 RFCPVSVILDEDSRPCGHKSLQDELGLMAAATTLRNALRNDHCRADEATEDLMNSYQKKI 316
           RF PV V +D D R    K +   +       T + +++ +    D  TE    +    +
Sbjct: 246 RFYPVRVTIDGDCRDYAQKHI-TRVRKPIPEFTAKKSVKEEIKTNDHHTEFTCITRNIGL 305

Query: 317 NGDVYINREEDDEDEEEDDEEEEDDDDAVSCSSSDLFELDNLSSIGIERYRQELPVYETT 370
              V  N+ E  E+EE          DA S SSSDLFELD+   IG+ RY +ELPVYETT
Sbjct: 306 KDFVRSNKYEGKEEEE----------DAWSHSSSDLFELDSY-RIGMGRYLKELPVYETT 336

BLAST of MC09g0195 vs. TAIR 10
Match: AT5G12050.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13980.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 114.8 bits (286), Expect = 1.5e-25
Identity = 138/374 (36.90%), Postives = 187/374 (50.00%), Query Frame = 0

Query: 18  RQNPSFSSSLLDAIYRSIDETNGVQGEEQVVFSMADFDRK----ARNVRDRDRDRNRGFK 77
           ++NPSFSS+LLD IY SID     Q  +  V S+    +K     R+V DR   R+R F 
Sbjct: 5   KRNPSFSSTLLDEIYNSIDPK--TQKTQPYVGSVNTTTKKQSIVTRSVPDRKIHRDRFF- 64

Query: 78  KLMNSNSSSSSSESSCGGGFSSSESE---------SRSSSYTMLRPRPIRTEKPQFDGHG 137
               S SSSS S SS    FSSS++E         SR   +   + +P +TE        
Sbjct: 65  ---GSVSSSSDSNSSI---FSSSDTELTHGKKTTSSRPLCFGPSKTKPRKTEDKTLFHQN 124

Query: 138 DAIKV--------SVPNQKPKHENGFVKTKSKALKIYGDLKKVKQPISPGGRFATFLNSL 197
            A +V         VP      EN       +++K  G+ KK K P SPGGR   FLNSL
Sbjct: 125 RATRVYDDYDYASDVPKFNRHDENWENTRNRRSVKSSGNQKKPKTPASPGGRIVNFLNSL 184

Query: 198 FNGGSPK----KQKFTNSAQQNDACMRKSKS---AQGSTCSSASSFSRSCLSKTPSSGEK 257
           F+  S +    K     ++  + A +RK+ +   +  +TCSSASSFSRSC++K     EK
Sbjct: 185 FSNNSKQSNAVKSYPRKTSYDDSAYVRKTSNDYHSSTTTCSSASSFSRSCMNK---GYEK 244

Query: 258 LINNGSTKRSVRFCPVSVILDEDSRPCGHKSLQDELGLMAAATTLRNALRNDHCRADE-- 317
             ++G  KRSVRF PV+VI+ E          +D      A  +++  + +   R+ E  
Sbjct: 245 --SSGRIKRSVRFSPVNVIVPESFT----SKEEDYFSNGNARKSVKKNVEDGGRRSVEEI 304

Query: 318 ATEDLMNSYQKKINGDVYINREEDDEDEEEDDEEEEDDDDAVSCSSSDLFELDNLSSIGI 361
           A E L + ++   N  V  N  ED ED++EDD    DDDD  S SSSDLFELD + +   
Sbjct: 305 AREFLRDYHKNHENSLVKTNGFEDYEDDDEDD----DDDDVASDSSSDLFELDLVGNHHH 356

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SLL24.4e-6247.14Protein BIG GRAIN 1-like B OS=Arabidopsis thaliana OX=3702 GN=At1g54200 PE=1 SV=... [more]
Q9LVK25.9e-5145.18Protein BIG GRAIN 1-like A OS=Arabidopsis thaliana OX=3702 GN=At3g13980 PE=3 SV=... [more]
Q9M2B31.1e-2534.42Protein BIG GRAIN 1-like C OS=Arabidopsis thaliana OX=3702 GN=At3g42800 PE=2 SV=... [more]
Q9LYH02.1e-2436.90Protein BIG GRAIN 1-like D OS=Arabidopsis thaliana OX=3702 GN=At5g12050 PE=2 SV=... [more]
Q10R099.2e-2033.06Protein BIG GRAIN 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BG1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022145624.11.09e-24588.46protein BIG GRAIN 1-like B [Momordica charantia][more]
XP_038896283.13.22e-20476.61protein BIG GRAIN 1-like B [Benincasa hispida][more]
KAG6593542.11.07e-20075.23Protein BIG GRAIN 1-like A, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7025884.11.07e-20075.23Protein BIG GRAIN 1-like A, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022964194.11.29e-19875.06protein BIG GRAIN 1-like B [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1CVT35.30e-24688.46protein BIG GRAIN 1-like B OS=Momordica charantia OX=3673 GN=LOC111015022 PE=3 S... [more]
A0A6J1HME76.25e-19975.06protein BIG GRAIN 1-like B OS=Cucurbita moschata OX=3662 GN=LOC111464284 PE=3 SV... [more]
A0A0A0K9K72.22e-19873.78Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G450580 PE=3 SV=1[more]
A0A6J1KKD71.09e-19573.83protein BIG GRAIN 1-like B OS=Cucurbita maxima OX=3661 GN=LOC111494822 PE=3 SV=1[more]
A0A6J1GM942.48e-19474.46protein BIG GRAIN 1-like B OS=Cucurbita moschata OX=3662 GN=LOC111455790 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT1G54200.13.1e-6347.14unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT3G13980.14.2e-5245.18unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT3G42800.18.0e-2734.42unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT5G12050.11.5e-2536.90unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 306..326
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 52..73
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 74..109
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 302..334
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 307..331
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..18
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 52..140
NoneNo IPR availablePANTHERPTHR33541:SF2PROTEIN BIG GRAIN 1-LIKE Acoord: 10..369
IPR039621Protein BIG GRAIN 1-likePANTHERPTHR33541PROTEIN BIG GRAIN 1-LIKE A-RELATEDcoord: 10..369

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g0195.1MC09g0195.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009734 auxin-activated signaling pathway
cellular_component GO:0005886 plasma membrane