MC09g0056 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC09g0056
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	alpha/beta-Hydrolases superfamily protein
Location	MC09: 537664 .. 541550 (+)
RNA-Seq Expression	MC09g0056
Synteny	MC09g0056

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAGTTTTCAACCAGCTTAGGTCTTGTTGAATGTTGTTTCATTTTTTTGGTGGGATTGCATTACAAAAAATGGGTTCCAAAGAAATCCAATTTGCCAGTGATTATCTGATACTGAAGCCGGAGGAAGCAACTCTGCTGGATCTGTTTCTCTTCGTACTTCCTTTTGGTTTCAAAAATCTGAGGAAGTTGGTGGATTGTCCCCCCGAAAAAGAAGAGTCTTACAACTCCTTTGGAAACCGATGGATTATCTTCTTCTCCATTTTGTGGCAGAAAATCCTTCTCGCATTCTTAAATCAGCACAAAGGTATATTTTCATAACGAGGGTCGGGATAAAATTTTATGGAAAATACTTGGCTCCTTAATCTTTTCCTCACCATCTTTGACAACTATTTGACAACTAAGGGATAGAAATGAAAAAAATAGACTTAAAAGAAAAAAAAAAACGAGTCTTTCTATGGTGGTGAAGACAAGATTTGATTCTTACTGATAAGGAGCCAAGTATTTTTCGAATTTTATTATACCCGTTTGGTAACATTAAAAAAACCTAAACATTTTGCAGTTAATTATATTTTTCTTATTTCTCATTTTTCGAAAACATCTTTAAAATTGTGTAGAAATTTTGAGAACGATCGGGCTCGTTTGGTAAGATTATCATTTCTTGTTTCTCGTTTTCAATTTTCTTAAAAAATCTAAACATTTTGCAACTAACTTTTTGTATAGAAATTTTGGAAACAACACAAACAAATTTTTTCTTTCTTGCTCTCATTTCTAAATTTGTTCATAAACATAAATAGAAAGGAAACATATTTTACCAAACAGACCCTAAGTTTCTTGTTTCTTGTTTTCGTCTTCAATTTGTTATAAAACATACATAATCATCTTTGAATTCATGAGTGTTGTGGCCTACAATTCTATTTTTATTGGTTAAAAATGAAGAAACAGAAATTAAAAAACGAGAATGTTATGAAACAGGTGTTTTAAGGACTGAGTTTTAGTTTTTAGTTAATTGAGTTTTGTTACGTGGGCATGCAGAAAAGCCAAAAACTGAGGATCCACCGACAGTGAACTGTAAAGATTGGAGTCTAAGTGGTCGGGATAAGAACATAAAATTAGACGACCCTTTCCAATATTATGCCATCCTCTCAGTCAGAGCTTCGGCTTTGGCGAGCGAAGACTTTTCACCTCATCCTTCTGTGGTCGAGACCGTCGTCAATTCCTGCTGGAATGTAAATACAATATAATCCTTACCTCTCTCTCTTCATTTTTAATGATTTGGGTTACTAAAAAGATTCCTATAGATCGGTTAAGAGGTAAGAATTGTTAAGGTTTATGTGTTATTTAGCCGATTGATTATAATAACTAGGGGTATAAGCCTTTGGCTCGACTTAGTTTGGGAGTCAAATCGAAGCCGAACTGATAATAAACAACGTATTAACAAAAAAAATGAGTCGAATCGATAGTGATCAATATAAGATCGAGTCACGCTGAACTAACAAACCCGTCGGTTTATTGGTTTGGTCAGGCGGTTTGATTTCCACATGTCCTTGGATTTTAGGGTAGCAAACCTATTGCGTCGTACTACTTGGATCTACGGTTGGTTAGAAGAGATGGAGTGAATGGGAGGGAGGGAGGTGGGAGAGAGTCGGGAGGGATGGTTAGGAGAAAGATTCCTATTGATCGGTAAAGAAGCAAGTATAATAAGTTAATAACTCACTAGATAAAGACAGTTATTACTCCTCTTATTTCTTAATTGTTTAAAGAAAAATAAAATTGAGCTATATATGTTCGTACTAATATATCAGATTATTGTTATAAAGTAGTATAACAGATATAACTTCAATTATTATCCTCGTTGAATACTTGGGAATAGTGATGAGAACTAATGATCATGACGTTTTATTCTTTTCAATGCAGATGAAACTACTTGGATGCTACGATCTTTGGAATGGTAAGACTTTGGACTGTCACAGTCATAATAAATTTAATTAATTAATGGTCTTAAATTGATTTAACATAAATGAATGATTAATATTCTAAACATCCAATAACTTATTTTAAAATGAAATACCAATTAACTGTGACCATAATGAATCCATATTGCAAAAGACTGCCTATTAGTGGAGATGATTGTATGATTTGTGTAAGATACATAGTTTTTTTCTTTCCTTTTCTTTTATGCAACAAGTGAGGTGCGAAAAATGACAGGTCACGGTGACAATTATCGATAAACTAAGTTCATGTTTGTTGTTTTAAATTTGAACACGATGGTTTTCAGACTTCCAAGACATGGGTTCGACTCAAGTGTTTATGTTTCAGAATACTGCCACAGATCCAAACGTAACAGTGGTGGCATTTAGAGGCACCACTGACGTTTATGATATGAAGGTAGATTTGGACTTTTCCTGGTACGAGATTGCAAATGTGGGCAAAATCCACAGAGGGTTCATGGAAGCCCTTGGCCTGCAGAAGCAGCTAGGTTGGCCTAAGGAACTCGTAAAACCCGAGCACGATTATGCATATTACTTCCTCCGTCAACAACTCAGAGACATTGCCAAATCCAACGACAAGGCAAGGTTCATCATCACTGGCCATAGCTTGGGCGCAGCCCTCGCCACCCTGTTCGTCACCATCCTCGCCTTCCACGAGGAACTCGATTTGTTAGAGAAGTTAAAGGCTGTTTACACCTTTGGCCAACCCAGAGTCGGCGATGTCCAGTTTGTTCAGTTTATGGAAAACGTTAATTCAAAAGTACATAAATTCCCATATTACAGGTACGTTTACTCTATGGACGTGGTTCCCAGAATTCCTTTCGATGTCGAAGGTGATCAATGGTACAAACACTTTGGCGGATGTCTTTACTACGACTGCAACTACACTGGGGAGGTAATTATGAACCTTCGGCTGCAACCGCTCTTTTTCTCTCTTAATTAAATTATTATCCGAAGAGTATATTGGATTTGTAAAAAGAGTACAAACAAAGTTCTACATTGGTCAGGAGAGGGGACGTGGTCATGATATATAACAGAGGATAATTATCTTCATAGACATTTTAGGTGAAACCAAAAGTAAAATCGTAAGAATTATGCCCAAAGCAGACAATATCAATTAGTTTTTCAATAGAGGATGAGACGACTATTTTGTTACAATCATATTGAATAACTTCTCTTTTTTAGCTTTTTCGGGAGAGTAAACTAATGTTTTGGGTGCAATATACGCAGTTTCTGGAGGAGGAGCCAAACAAGAACTACTTGTCAATACCTTGGCTAATACCCAAATACTTGTACGCTTGGTGGGAGTTGCTAAGAAGTCTACTGCTCCCAGTAATCATGTGTAATCCGGATTACTTTGAGACTTCTCAGATTTTCTATGCCAGGGTTTTTGGGCTGTTTATTCCAGGAGCATCAGCTCACAACGTTGTGAACTATATTTACTCGGCCCGTTGGGGAAAAATAGTATGCAGAGACACCCAGAAAAAAGTAATTCCTGCAGACTATCTTGAACGCTGCTGATCGGTGCCCGAATATGAAGAAGAAGATAGAAGATATCATATATAATGAAAATACTTGGTCCCTCAACAGTAAGTAGCAATCTTGTCCTACCATATAAGAAAAAGAATACAAAAATTGAATGTCAATATTTTAATGGTGGTGAAGACAAGATTTTATCCTTGCCGTCTTAGGAACCAAGTATTTTAATTTCCTAATATGTTTGTAATGTGACTTTGCCTAAAATAAGTGTGTTTGTTTGTTAGTGTATTAAGTTTGTACGTATTTCTAAAAACTGCTCCTTTGGGTTTGGGTTTGTAGCTCATGAAATAAATAAACTCATGTGCCAATTCTACATGGCTCAGCTTTCTTAATCTTAATTAGTTTATGCAAATCTAAC

mRNA sequence

AAAAAGTTTTCAACCAGCTTAGGTCTTGTTGAATGTTGTTTCATTTTTTTGGTGGGATTGCATTACAAAAAATGGGTTCCAAAGAAATCCAATTTGCCAGTGATTATCTGATACTGAAGCCGGAGGAAGCAACTCTGCTGGATCTGTTTCTCTTCGTACTTCCTTTTGGTTTCAAAAATCTGAGGAAGTTGGTGGATTGTCCCCCCGAAAAAGAAGAGTCTTACAACTCCTTTGGAAACCGATGGATTATCTTCTTCTCCATTTTGTGGCAGAAAATCCTTCTCGCATTCTTAAATCAGCACAAAGAACGATCGGGCTCGTTTGGTAAGATTATCATTTCTTGTTTCTCGTTTTCAATTTTCTTAAAAAATCTAAACATTTTGCAACTAACTTTTTTTTTGTTACGTGGGCATGCAGAAAAGCCAAAAACTGAGGATCCACCGACAGTGAACTGTAAAGATTGGAGTCTAAGTGGTCGGGATAAGAACATAAAATTAGACGACCCTTTCCAATATTATGCCATCCTCTCAGTCAGAGCTTCGGCTTTGGCGAGCGAAGACTTTTCACCTCATCCTTCTGTGGTCGAGACCGTCGTCAATTCCTGCTGGAATATGAAACTACTTGGATGCTACGATCTTTGGAATGACTTCCAAGACATGGGTTCGACTCAAGTGTTTATGTTTCAGAATACTGCCACAGATCCAAACGTAACAGTGGTGGCATTTAGAGGCACCACTGACGTTTATGATATGAAGGTAGATTTGGACTTTTCCTGGTACGAGATTGCAAATGTGGGCAAAATCCACAGAGGGTTCATGGAAGCCCTTGGCCTGCAGAAGCAGCTAGGTTGGCCTAAGGAACTCGTAAAACCCGAGCACGATTATGCATATTACTTCCTCCGTCAACAACTCAGAGACATTGCCAAATCCAACGACAAGGCAAGGTTCATCATCACTGGCCATAGCTTGGGCGCAGCCCTCGCCACCCTGTTCGTCACCATCCTCGCCTTCCACGAGGAACTCGATTTGTTAGAGAAGTTAAAGGCTGTTTACACCTTTGGCCAACCCAGAGTCGGCGATGTCCAGTTTGTTCAGTTTATGGAAAACGTTAATTCAAAAGTACATAAATTCCCATATTACAGGTACGTTTACTCTATGGACGTGGTTCCCAGAATTCCTTTCGATGTCGAAGGTGATCAATGGTACAAACACTTTGGCGGATGTCTTTACTACGACTGCAACTACACTGGGGAGTTTCTGGAGGAGGAGCCAAACAAGAACTACTTGTCAATACCTTGGCTAATACCCAAATACTTGTACGCTTGGTGGGAGTTGCTAAGAAGTCTACTGCTCCCAGTAATCATGTGTAATCCGGATTACTTTGAGACTTCTCAGATTTTCTATGCCAGGGTTTTTGGGCTGTTTATTCCAGGAGCATCAGCTCACAACGTTGTGAACTATATTTACTCGGCCCGTTGGGGAAAAATAGTATGCAGAGACACCCAGAAAAAAGTAATTCCTGCAGACTATCTTGAACGCTGCTGATCGGTGCCCGAATATGAAGAAGAAGATAGAAGATATCATATATAATGAAAATACTTGGTCCCTCAACAGTAAGTAGCAATCTTGTCCTACCATATAAGAAAAAGAATACAAAAATTGAATGTCAATATTTTAATGGTGGTGAAGACAAGATTTTATCCTTGCCGTCTTAGGAACCAAGTATTTTAATTTCCTAATATGTTTGTAATGTGACTTTGCCTAAAATAAGTGTGTTTGTTTGTTAGTGTATTAAGTTTGTACGTATTTCTAAAAACTGCTCCTTTGGGTTTGGGTTTGTAGCTCATGAAATAAATAAACTCATGTGCCAATTCTACATGGCTCAGCTTTCTTAATCTTAATTAGTTTATGCAAATCTAAC

Coding sequence (CDS)

ATGTTGTTTCATTTTTTTGGTGGGATTGCATTACAAAAAATGGGTTCCAAAGAAATCCAATTTGCCAGTGATTATCTGATACTGAAGCCGGAGGAAGCAACTCTGCTGGATCTGTTTCTCTTCGTACTTCCTTTTGGTTTCAAAAATCTGAGGAAGTTGGTGGATTGTCCCCCCGAAAAAGAAGAGTCTTACAACTCCTTTGGAAACCGATGGATTATCTTCTTCTCCATTTTGTGGCAGAAAATCCTTCTCGCATTCTTAAATCAGCACAAAGAACGATCGGGCTCGTTTGGTAAGATTATCATTTCTTGTTTCTCGTTTTCAATTTTCTTAAAAAATCTAAACATTTTGCAACTAACTTTTTTTTTGTTACGTGGGCATGCAGAAAAGCCAAAAACTGAGGATCCACCGACAGTGAACTGTAAAGATTGGAGTCTAAGTGGTCGGGATAAGAACATAAAATTAGACGACCCTTTCCAATATTATGCCATCCTCTCAGTCAGAGCTTCGGCTTTGGCGAGCGAAGACTTTTCACCTCATCCTTCTGTGGTCGAGACCGTCGTCAATTCCTGCTGGAATATGAAACTACTTGGATGCTACGATCTTTGGAATGACTTCCAAGACATGGGTTCGACTCAAGTGTTTATGTTTCAGAATACTGCCACAGATCCAAACGTAACAGTGGTGGCATTTAGAGGCACCACTGACGTTTATGATATGAAGGTAGATTTGGACTTTTCCTGGTACGAGATTGCAAATGTGGGCAAAATCCACAGAGGGTTCATGGAAGCCCTTGGCCTGCAGAAGCAGCTAGGTTGGCCTAAGGAACTCGTAAAACCCGAGCACGATTATGCATATTACTTCCTCCGTCAACAACTCAGAGACATTGCCAAATCCAACGACAAGGCAAGGTTCATCATCACTGGCCATAGCTTGGGCGCAGCCCTCGCCACCCTGTTCGTCACCATCCTCGCCTTCCACGAGGAACTCGATTTGTTAGAGAAGTTAAAGGCTGTTTACACCTTTGGCCAACCCAGAGTCGGCGATGTCCAGTTTGTTCAGTTTATGGAAAACGTTAATTCAAAAGTACATAAATTCCCATATTACAGGTACGTTTACTCTATGGACGTGGTTCCCAGAATTCCTTTCGATGTCGAAGGTGATCAATGGTACAAACACTTTGGCGGATGTCTTTACTACGACTGCAACTACACTGGGGAGTTTCTGGAGGAGGAGCCAAACAAGAACTACTTGTCAATACCTTGGCTAATACCCAAATACTTGTACGCTTGGTGGGAGTTGCTAAGAAGTCTACTGCTCCCAGTAATCATGTGTAATCCGGATTACTTTGAGACTTCTCAGATTTTCTATGCCAGGGTTTTTGGGCTGTTTATTCCAGGAGCATCAGCTCACAACGTTGTGAACTATATTTACTCGGCCCGTTGGGGAAAAATAGTATGCAGAGACACCCAGAAAAAAGTAATTCCTGCAGACTATCTTGAACGCTGCTGA

Protein sequence

MLFHFFGGIALQKMGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWIIFFSILWQKILLAFLNQHKERSGSFGKIIISCFSFSIFLKNLNILQLTFFLLRGHAEKPKTEDPPTVNCKDWSLSGRDKNIKLDDPFQYYAILSVRASALASEDFSPHPSVVETVVNSCWNMKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVAFRGTTDVYDMKVDLDFSWYEIANVGKIHRGFMEALGLQKQLGWPKELVKPEHDYAYYFLRQQLRDIAKSNDKARFIITGHSLGAALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFPYYRYVYSMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPKYLYAWWELLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASAHNVVNYIYSARWGKIVCRDTQKKVIPADYLERC

Homology

[Close]

BLAST of MC09g0056 vs. ExPASy Swiss-Prot
Match: F4JFU8 (Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 2.8e-67
Identity = 174/506 (34.39%), Postives = 258/506 (50.99%), Query Frame = 0


Query: 23  SDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEES-----YNSFGNRWIIFFSI 82
           S+YLI++P     +DLF + +     +  K ++ P  +E S       +  +RW+I  SI
Sbjct: 17  SNYLIVRPHRGGYIDLFRYGVRDDQTSKAKFLEMPDNREWSTITIDEEAEDHRWVIVVSI 76

Query: 83  LWQKILLAFLNQHKERSGSFGKIIISCFSFSIFLKNLNILQLTFFLLRGHAEKPKTEDPP 142
           L +KI +  L    E +G      +  F  ++F  N     L   L++     P+     
Sbjct: 77  LVRKI-IRLLRTPMEFTG-----FVVDFFLNLFSANGGFFGLLLRLIQAKVVIPERGSVT 136

Query: 143 TVNC-----------KDWS----LSGRDK----NIKLDDPFQYYAILSVRASALASEDFS 202
            V+            K+W+    L G D      +K++   +    L V AS LA E   
Sbjct: 137 FVSTIGQLDGRISLYKEWNFVEHLEGIDSVDSGRVKIELGSRGLMDLCVMASKLAYE--- 196

Query: 203 PHPSVVETVVNSCWNMKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVAFRGTT--D 262
            +  VVE VV+  W M L+   D WND+Q   STQVF+F +   D N+ V++FRGT   D
Sbjct: 197 -NAKVVENVVDLHWKMNLVEFLDCWNDYQKQMSTQVFVFTDKQKDANLIVISFRGTEPFD 256

Query: 263 VYDMKVDLDFSWYEIANVGKIHRGFMEALGL------------------QKQLGWPKELV 322
             D   D D+SWYE+ NVGK+H GF+EA+GL                   ++    K L+
Sbjct: 257 ADDWGTDFDYSWYEVPNVGKLHMGFLEAMGLGNRDDTTTFHYNLFEQTSSEEENSKKNLL 316

Query: 323 KPEHDYAYYFLRQQLRDIAKSNDKARFIITGHSLGAALATLFVTILAFHEELDLLEKLKA 382
                 AYY +R  L+ +   ++ ARF++TGHSLG ALA LF T+L  +EE +++++L  
Sbjct: 317 DMVERSAYYAVRVILKRLLSEHENARFVVTGHSLGGALAILFPTLLVLNEETEIMKRLLG 376

Query: 383 VYTFGQPRVGDVQFVQFME-NVNSKVHKFPYYRYVYSMDVVPRIPFDVEGDQWYKHFGGC 442
           VYTFGQPR+G+ +   FM+  +N  V +  Y+R VY  D+VPR+P+D +    YKHFG C
Sbjct: 377 VYTFGQPRIGNREVGLFMKAQLNQPVDR--YFRVVYCNDIVPRLPYD-DKTFLYKHFGLC 436

Query: 443 LYYDCNYTGEFLEEEPNKNYLSIPWLIPKYLYAWWELLRSLLLPVIMCNPDYFETSQIFY 484
           L+YD  Y     E+EP+ N   + + I  ++ A WEL+R L +      PDY E      
Sbjct: 437 LFYDSFYNETKAEDEPDPNPYGLRYKILGHVIAVWELVRGLTMGYTH-GPDYKEGWFRIL 496

[Close]

BLAST of MC09g0056 vs. ExPASy Swiss-Prot
Match: A0A1S3ZP85 (Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 1.4e-66
Identity = 175/501 (34.93%), Postives = 244/501 (48.70%), Query Frame = 0


Query: 69  NRWIIFFSILWQKILLAFLNQHKERSGSFGKIIISCFSFSIFLKNLNILQLTFFLLRGHA 128
           +RW+IF SI+ +K L+A   +  E +G   +  ++ FS      N N L L + +L G  
Sbjct: 85  HRWVIFVSIIVRK-LIAIFGKPMEWTGYLVEFFLNLFSL-----NGNFLGLLYNILHGKV 144

Query: 129 EKPKTEDPPTVNCKDWSLSGR---------DKNIKLDDPFQYYAI-------LSVRASAL 188
             P       ++     L GR          K I   D +Q   I       L + AS L
Sbjct: 145 VMPHRGSETFISAIG-HLDGRINLYKSETLTKEIGEPDFWQKIGIGHRDLMDLCMMASKL 204

Query: 189 ASEDFSPHPSVVETVVNSCWNMKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVAFR 248
           A E    +  VV  VVN  W M  +  Y+ WNDF+   STQVF+  +   D N+ +V+FR
Sbjct: 205 AYE----NEKVVRNVVNLHWKMHFVDFYNCWNDFEKEMSTQVFLLCDKPKDANLILVSFR 264

Query: 249 GTT--DVYDMKVDLDFSWYEIANVGKIHRGFMEALGLQKQLGW----------------- 308
           GT   D  D   D D+SWYEI  +GK+H GF+EALGL  +                    
Sbjct: 265 GTEPFDADDWITDFDYSWYEIPKLGKVHMGFLEALGLGNRTNASTFHEQLFVNNLKFANL 324

Query: 309 --------PKELVKPEHDY----------------------------------AYYFLRQ 368
                   P E  K    +                                  AYY +R 
Sbjct: 325 ENVHATIPPSESSKSSTSFSDSDAHTGSDLSSDSERPTDTRKKKFRLEIPERTAYYVVRS 384

Query: 369 QLRDIAKSNDKARFIITGHSLGAALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQ 428
           +L+ + K +  A+F++TGHSLG ALA LF  +L  HEE+D++E+L  +YT+GQPRVG+ Q
Sbjct: 385 KLKRLLKEHKNAKFVVTGHSLGGALAILFPAVLVLHEEVDVMERLLGIYTYGQPRVGNRQ 444

Query: 429 FVQFME-NVNSKVHKFPYYRYVYSMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLE 488
             +FME ++   V K  Y+R VY  D+VPR+P+D      +KHFG C YY+  Y  + + 
Sbjct: 445 LGRFMEAHLEHPVPK--YFRVVYCNDLVPRLPYD-NKTFLFKHFGICQYYNSLYIEQNIN 504

Query: 489 EEPNKNYLSIPWLIPKYLYAWWELLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASA 492
           EEPN NY  + +L+P YL A WEL+RS  +   M   +Y E  +    R  GLF+PG SA
Sbjct: 505 EEPNPNYFGMRFLVPLYLNAGWELIRSFTMG-YMYGSEYEECWESVMLRALGLFLPGISA 564

[Close]

BLAST of MC09g0056 vs. ExPASy Swiss-Prot
Match: Q5VKJ7 (Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 2.3e-58
Identity = 163/511 (31.90%), Postives = 247/511 (48.34%), Query Frame = 0


Query: 22  ASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRW--IIFFSILW 81
           ++ +LI+ P+E T LDLF  ++     +  K      ++  +  ++ +RW   +F   + 
Sbjct: 9   STSHLIVSPDEGTFLDLFKHIVLSDLGSGAKFFRASDQRVPATAAYYSRWPVSVFICKIL 68

Query: 82  QKILLA----------FLNQHKERSGSFGKIIISCFSFSI---------FLKNLNILQLT 141
           Q   +            LN + +  G  G I+ + F   +         F+  +  L  +
Sbjct: 69  QLFQMPAAMLGHLTDFLLNFYYQNHGFLG-ILRNIFLIRLKIPKRGEADFISTIGYLD-S 128

Query: 142 FFLLRGHAEKPKTEDPPTVNCKDWSL-SGRDKNIKLDDPFQYYAILSVRASALASEDFSP 201
              L G        D    N  + SL  G +  IK     +    L + AS LA E    
Sbjct: 129 RMDLHGTPMVSHQADEVISNADNPSLKEGHNSKIKGALGNRSLMDLCIMASKLAYE---- 188

Query: 202 HPSVVETVVNSCWNMKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVAFRGT--TDV 261
           +  VVE VV   W M  +  Y   N FQD  +T  F+F +   D N+ V++FRGT    +
Sbjct: 189 NTKVVERVVAEHWKMHFVADYGGMNYFQDARNTHAFIFCDKPKDANLIVISFRGTGPFSI 248

Query: 262 YDMKVDLDFSWYEIANVGKIHRGFMEALGL--------------QKQLGWPKELVK---- 321
            +   D DFS   + + G +H GF+EA+GL               K  G   EL K    
Sbjct: 249 PNWCTDFDFSLVGLGDAGSVHVGFLEAMGLGHRNSISSFETSINTKSPGSITELRKESEM 308

Query: 322 -PEH-----DYAYYFLRQQLRDIAKSNDKARFIITGHSLGAALATLFVTILAFHEELDLL 381
            P+H     D  Y+     L+ + K +  A+F++TGHSLG ALA LF  IL   +E ++L
Sbjct: 309 APDHLVWAYDGVYFLAASTLKGLLKDHKNAKFVVTGHSLGGALAILFTCILEIQQETEVL 368

Query: 382 EKLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFPYYRYVYSMDVVPRIPFDVEGDQWYKH 441
           ++L  VYTFGQPR+G+     FM+N      +  Y+R VY  D+VPR+PFD +    ++H
Sbjct: 369 DRLLNVYTFGQPRIGNYNLGYFMQN-RLNFPERRYFRVVYCNDMVPRVPFD-DVFFTFEH 428

Query: 442 FGGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPKYLYAWWELLRSLLLPVIMCNPDYFETS 485
           FG C+YYD  + G F +EEP++N   I   I  ++ AWWEL RS +L  +    +Y ET 
Sbjct: 429 FGTCIYYDSRFFGYFTKEEPSRNPFGIENAISAHITAWWELWRSFILNHVY-GAEYKETW 488

[Close]

BLAST of MC09g0056 vs. ExPASy Swiss-Prot
Match: O59952 (Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.1e-07
Identity = 60/238 (25.21%), Postives = 105/238 (44.12%), Query Frame = 0


Query: 149 RDKNIKLDDPFQYYAILSVRASALASEDFSPHPSVVETVVNSCWNMKLLGCYDLWNDFQD 208
           R+ +  L + F  +A  S  A    + D +P  + +    N+C  ++      L++ F+D
Sbjct: 22  REVSQDLFNQFNLFAQYSAAAYCGKNND-APAGTNITCTGNACPEVEKADATFLYS-FED 81

Query: 209 MGSTQVFMFQNTATDPNVTVVAFRGTTDVYDMKVDLDFSWYEIANV---GKIHRGFMEAL 268
            G   V  F        + V++FRG+  + +   +L+F   EI ++    + H GF  + 
Sbjct: 82  SGVGDVTGFLALDNTNKLIVLSFRGSRSIENWIGNLNFDLKEINDICSGCRGHDGFTSS- 141

Query: 269 GLQKQLGWPKELVKPEHDYAYYFLRQQLRDIAKSNDKARFIITGHSLGAALATLFVTILA 328
                  W               LRQ++ D  + +   R + TGHSLG ALAT+    L 
Sbjct: 142 -------WRS---------VADTLRQKVEDAVREHPDYRVVFTGHSLGGALATVAGADLR 201

Query: 329 FH-EELDLLEKLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFPYYRYVYSMDVVPRIP 383
            +  ++D       V+++G PRVG+  F +F+            YR  ++ D+VPR+P
Sbjct: 202 GNGYDID-------VFSYGAPRVGNRAFAEFLTVQTGGT----LYRITHTNDIVPRLP 229

[Close]

BLAST of MC09g0056 vs. ExPASy Swiss-Prot
Match: P19515 (Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2)

HSP 1 Score: 59.3 bits (142), Expect = 1.4e-07
Identity = 47/156 (30.13%), Postives = 69/156 (44.23%), Query Frame = 0


Query: 229 VAFRGTTDVYDMKVDLDF--SWYEIANVGKIHRGFMEALGLQKQLGWPKELVKPEHDYAY 288
           + FRG++ + +   DL F    Y   +  K+H+GF+++ G  +      ELV        
Sbjct: 171 IVFRGSSSIRNWIADLTFVPVSYPPVSGTKVHKGFLDSYGEVQ-----NELV-------- 230

Query: 289 YFLRQQLRDIAKSNDKARFIITGHSLGAALATLFVTILAFHEELDLLEKLKAVYTFGQPR 348
                 + D  K     +  +TGHSLG A A L    L +  E  L      +YT GQPR
Sbjct: 231 ----ATVLDQFKQYPSYKVAVTGHSLGGATALLCALDL-YQREEGLSSSNLFLYTQGQPR 290

Query: 349 VGDVQFVQFMENVNSKVHKFPYYRYVYSMDVVPRIP 383
           VGD  F  ++ +        PY R V   D+VP +P
Sbjct: 291 VGDPAFANYVVSTG-----IPYRRTVNERDIVPHLP 303

[Close]

BLAST of MC09g0056 vs. NCBI nr
Match: XP_022152547.1 (uncharacterized protein LOC111020243 [Momordica charantia])

HSP 1 Score: 929 bits (2401), Expect = 0.0
Identity = 452/490 (92.24%), Postives = 453/490 (92.45%), Query Frame = 0


Query: 14  MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII 73
           MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII
Sbjct: 1   MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII 60

Query: 74  FFSILWQKILLAFLNQHKERSGSFGKIIISCFSFSIFLKNLNILQLTFFLLRGHAEKPKT 133
           FFSILWQKILLAFLNQHKE                                     KPKT
Sbjct: 61  FFSILWQKILLAFLNQHKE-------------------------------------KPKT 120

Query: 134 EDPPTVNCKDWSLSGRDKNIKLDDPFQYYAILSVRASALASEDFSPHPSVVETVVNSCWN 193
           EDPPTVNCKDWSLSGRDKNIKLDDPFQYYAILSVRASALA+EDFSPHPSVVETVVNSCWN
Sbjct: 121 EDPPTVNCKDWSLSGRDKNIKLDDPFQYYAILSVRASALATEDFSPHPSVVETVVNSCWN 180

Query: 194 MKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVAFRGTTDVYDMKVDLDFSWYEIAN 253
           MKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVAFRGTTDVYDMKVDLDFSWYEIAN
Sbjct: 181 MKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVAFRGTTDVYDMKVDLDFSWYEIAN 240

Query: 254 VGKIHRGFMEALGLQKQLGWPKELVKPEHDYAYYFLRQQLRDIAKSNDKARFIITGHSLG 313
           VGKIHRGFMEALGLQKQLGWPKELVKPEHDYAYYFLRQQLRDIAKSNDKARFIITGHSLG
Sbjct: 241 VGKIHRGFMEALGLQKQLGWPKELVKPEHDYAYYFLRQQLRDIAKSNDKARFIITGHSLG 300

Query: 314 AALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFPYYRYVY 373
           AALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFPYYRYVY
Sbjct: 301 AALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFPYYRYVY 360

Query: 374 SMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPKYLYAWWE 433
           SMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPKYLYAWWE
Sbjct: 361 SMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPKYLYAWWE 420

Query: 434 LLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASAHNVVNYIYSARWGKIVCRDTQKK 493
           LLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASAHNVVNYIYSARWGKIVCRDTQKK
Sbjct: 421 LLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASAHNVVNYIYSARWGKIVCRDTQKK 453

Query: 494 VIPADYLERC 503
           VIPADYLERC
Sbjct: 481 VIPADYLERC 453

[Close]

BLAST of MC09g0056 vs. NCBI nr
Match: XP_022139747.1 (uncharacterized protein LOC111010582 [Momordica charantia])

HSP 1 Score: 595 bits (1534), Expect = 2.56e-208
Identity = 296/487 (60.78%), Postives = 348/487 (71.46%), Query Frame = 0


Query: 14  MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII 73
           MGS+E +F+SDYLILKPEEATLL LFLF LPFG++ LR LVDCP  KE SY +FGNRW+I
Sbjct: 1   MGSQENKFSSDYLILKPEEATLLHLFLFTLPFGYRKLRNLVDCPVAKENSYTTFGNRWVI 60

Query: 74  FFSILWQKILLAFLNQHKERSGSFGKIIISCFSFSIFLKNLNILQLTFFLLRGHAEKPKT 133
           F SIL QK LLA  N  K      GK                                KT
Sbjct: 61  FISILLQKFLLAIANLIKYCKSMRGKF------------------------------HKT 120

Query: 134 EDPPTVNCKDWSLSGRDKNIKL--DDPFQYYAILSVRASALASEDF-SPHPSVVETVVNS 193
              P VNCK+W + G +KNIKL  DD F+YY  L+V AS LA +D+ SP PSVV+TVVN 
Sbjct: 121 CAAPKVNCKEWKIIGMEKNIKLADDDTFKYYGALTVMASNLAYQDYNSPEPSVVQTVVNG 180

Query: 194 CWNMKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVAFRGTTDVYDMKVDLDFSWYE 253
           CW M LLGCYD WNDFQ+  +TQVFMFQNTATDPNVTVVAFRG++++YD  VDL+ SWY 
Sbjct: 181 CWKMNLLGCYDFWNDFQNKATTQVFMFQNTATDPNVTVVAFRGSSEIYDWMVDLNLSWYN 240

Query: 254 IANVGKIHRGFMEALGLQKQLGWPKELVKPEHDYAYYFLRQQLRDIAKSNDKARFIITGH 313
           I  +GKIH GFM+ALGLQK  GWPKEL  P+HD+AYY LRQQLRDI KSND ARFI TGH
Sbjct: 241 IEGIGKIHGGFMQALGLQKGTGWPKELANPDHDFAYYTLRQQLRDIVKSNDNARFIFTGH 300

Query: 314 SLGAALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFPYYR 373
           SLG ALA LF T+LAFH++  LLEKL+AVYTFGQPRVGD +F QFMEN   K + F YYR
Sbjct: 301 SLGGALAVLFATLLAFHDDATLLEKLQAVYTFGQPRVGDRRFAQFMENTIEK-YGFKYYR 360

Query: 374 YVYSMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPKYLYA 433
           YVYS D+VPR+PFD   + WY+HFGGC+Y++C+Y G+FLE +PNKNY S  WLI KY+ A
Sbjct: 361 YVYSSDLVPRVPFDFNDNLWYEHFGGCVYFNCSYNGKFLEVQPNKNYFSPIWLISKYVSA 420

Query: 434 WWELLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASAHNVVNYIYSARWGKIVCRDT 493
           WWEL+RSL++PVI C+ DY E       R+FGL  PGASAH  +NYI S RWGK    + 
Sbjct: 421 WWELIRSLMIPVIKCSSDYLEGFSNLLLRLFGLLFPGASAHMCLNYINSIRWGKC---NH 453

Query: 494 QKKVIPA 497
           +K +IPA
Sbjct: 481 EKTLIPA 453

[Close]

BLAST of MC09g0056 vs. NCBI nr
Match: XP_022135571.1 (uncharacterized protein LOC111007483 isoform X2 [Momordica charantia])

HSP 1 Score: 566 bits (1458), Expect = 1.31e-196
Identity = 296/495 (59.80%), Postives = 358/495 (72.32%), Query Frame = 0


Query: 14  MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII 73
           MG ++ QF++DYLILKPEEATLLDLFLF+LPFG+  LRKLVDCP EKEESY SFGNRW+I
Sbjct: 1   MGPEDYQFSNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEKEESYKSFGNRWVI 60

Query: 74  FFSILWQKILLAFLNQHKERSGSFGKIIISCFSFSIFLKNLNILQLTFFLLRGHAEKPKT 133
           F SIL QK LLA  N  ++     GK                     F+ ++ +A +   
Sbjct: 61  FISILSQKFLLAIANLLEKWEPMRGK---------------------FWRVQTYASE--- 120

Query: 134 EDPPTVNCKDWSLSGRDKNIKL---DDPFQYYAILSVRASALASEDFSP-HPSVVETVVN 193
                VNC+DW + G+++NIKL   DD F YY  ++V AS L  +D SP   SVV TVVN
Sbjct: 121 -----VNCRDWKI-GKEENIKLLNDDDTFVYYGAVTVMASNLVYQDCSPPESSVVATVVN 180

Query: 194 SCWNMKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVAFRGTTDVYDMKVDLDFSWY 253
           SCW MKLLGCYD  N F+    TQ  MFQNTATDPNVTVVAF+GT  +YD  +DL+FSWY
Sbjct: 181 SCWKMKLLGCYDFENMFESKARTQATMFQNTATDPNVTVVAFKGTNTIYDAILDLNFSWY 240

Query: 254 EI-ANVGKIHRGFMEALGLQK-QLGWPKELVKPEHDYAYYFLRQQLRDIAKSNDKARFII 313
            +   +G IH GFMEALGLQK  +GWPKEL K +H +AYY+LRQQLRDIAKSNDKA+FI 
Sbjct: 241 NVDQRIGNIHSGFMEALGLQKGTIGWPKELPKSDHQFAYYYLRQQLRDIAKSNDKAKFIF 300

Query: 314 TGHSLGAALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFP 373
           TGHSLG ALA LFVTIL++H E D+L KL+AVYTFGQPRVG+ QFV+FME+  SK + F 
Sbjct: 301 TGHSLGGALAILFVTILSYHNESDVLNKLQAVYTFGQPRVGNSQFVKFMEST-SKQYAFK 360

Query: 374 YYRYVYSMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPKY 433
           YYRYVYSMD+VPRIPFD+  D+WYKHFGGC+Y++C Y+G+F+E +PNKNY S  WLIPKY
Sbjct: 361 YYRYVYSMDLVPRIPFDIF-DEWYKHFGGCVYFNCCYSGQFMEVQPNKNYFSPLWLIPKY 420

Query: 434 LYAWWELLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASAHNVVNYIYSARWGKIV- 493
           L+A WEL+RSL++P+I  +  YFE  +    R FGL IPGASAH  VNYIY AR GK+  
Sbjct: 421 LFALWELIRSLMMPLIKFSLYYFEGFKTLLLRFFGLLIPGASAHLCVNYIYLARQGKMPP 463

Query: 494 CRDTQKKVIPADYLE 501
            +D QK +I  D LE
Sbjct: 481 LKDIQKTLISEDGLE 463

[Close]

BLAST of MC09g0056 vs. NCBI nr
Match: XP_022135562.1 (uncharacterized protein LOC111007483 isoform X1 [Momordica charantia])

HSP 1 Score: 557 bits (1435), Expect = 4.19e-193
Identity = 295/495 (59.60%), Postives = 354/495 (71.52%), Query Frame = 0


Query: 14  MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII 73
           MGS E +F++DYL LKP+EATLLDLF+F+LPFG   LRKLVDCPPEKEESY SF NRW+I
Sbjct: 1   MGSLEYEFSNDYLKLKPKEATLLDLFIFLLPFGSIKLRKLVDCPPEKEESYTSFTNRWVI 60

Query: 74  FFSILWQKILLAFLNQHKERSGSFGKIIISCFSFSIFLKNLNILQLTFFLLRGHAEKPKT 133
           FFSIL QK L A  N  ++   S  K                        LR   +   +
Sbjct: 61  FFSILSQKSLFALANLLEKWESSTEK------------------------LRTAPQTYAS 120

Query: 134 EDPPTVNCKDWSLSGRDKNIKL---DDPFQYYAILSVRASALASEDFSP-HPSVVETVVN 193
           E    VNC+DW + G+++NIKL   DD F YY  ++V AS L  +D SP   SVV TVVN
Sbjct: 121 E----VNCRDWKI-GKEENIKLLNDDDTFVYYGAVTVMASNLVYQDCSPPESSVVATVVN 180

Query: 194 SCWNMKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVAFRGTTDVYDMKVDLDFSWY 253
           SCW MKLLGCYD  N F+    TQ  MFQNTATDPNVTVVAF+GT  +YD  +DL+FSWY
Sbjct: 181 SCWKMKLLGCYDFENMFESKARTQATMFQNTATDPNVTVVAFKGTNTIYDAILDLNFSWY 240

Query: 254 EI-ANVGKIHRGFMEALGLQK-QLGWPKELVKPEHDYAYYFLRQQLRDIAKSNDKARFII 313
            +   +G IH GFMEALGLQK  +GWPKEL K +H +AYY+LRQQLRDIAKSNDKA+FI 
Sbjct: 241 NVDQRIGNIHSGFMEALGLQKGTIGWPKELPKSDHQFAYYYLRQQLRDIAKSNDKAKFIF 300

Query: 314 TGHSLGAALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFP 373
           TGHSLG ALA LFVTIL++H E D+L KL+AVYTFGQPRVG+ QFV+FME+  SK + F 
Sbjct: 301 TGHSLGGALAILFVTILSYHNESDVLNKLQAVYTFGQPRVGNSQFVKFMEST-SKQYAFK 360

Query: 374 YYRYVYSMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPKY 433
           YYRYVYSMD+VPRIPFD+  D+WYKHFGGC+Y++C Y+G+F+E +PNKNY S  WLIPKY
Sbjct: 361 YYRYVYSMDLVPRIPFDIF-DEWYKHFGGCVYFNCCYSGQFMEVQPNKNYFSPLWLIPKY 420

Query: 434 LYAWWELLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASAHNVVNYIYSARWGKIV- 493
           L+A WEL+RSL++P+I  +  YFE  +    R FGL IPGASAH  VNYIY AR GK+  
Sbjct: 421 LFALWELIRSLMMPLIKFSLYYFEGFKTLLLRFFGLLIPGASAHLCVNYIYLARQGKMPP 464

Query: 494 CRDTQKKVIPADYLE 501
            +D QK +I  D LE
Sbjct: 481 LKDIQKTLISEDGLE 464

[Close]

BLAST of MC09g0056 vs. NCBI nr
Match: XP_022132571.1 (uncharacterized protein LOC111005368 isoform X1 [Momordica charantia])

HSP 1 Score: 511 bits (1317), Expect = 1.37e-175
Identity = 268/479 (55.95%), Postives = 330/479 (68.89%), Query Frame = 0


Query: 14  MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII 73
           MGS++ QF+ DYLILKPEEATLLDLFLF+LPFGF+ +RKLVDCP  KE SY S  +R II
Sbjct: 1   MGSEDHQFSDDYLILKPEEATLLDLFLFILPFGFRKIRKLVDCPAAKENSYRSIQSRLII 60

Query: 74  FFSILWQKILLAFLNQHKERSGSFGKIIISCFSFSIFLKNLNILQLTFFLLRGH----AE 133
           F SIL+QK++LA  N  K+                            F L+RG     + 
Sbjct: 61  FVSILFQKLVLAISNLAKK----------------------------FKLMRGKLWAVSY 120

Query: 134 KPKTEDPPTVNCKDWSLSGRDKNIKL-DDPFQYYAILSVRASALASEDFSPHPSVVETVV 193
           KP     P VNC+DW + G +KN+KL +D F+YY+ L+V AS LA  D+SP P++V+TVV
Sbjct: 121 KPYA---PKVNCRDWKIGG-EKNVKLPEDNFKYYSALTVMASQLAYTDYSPQPNIVQTVV 180

Query: 194 NSCWNMKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVAFRGTTDVYDMKVDLDFSW 253
             CW M L+  Y   N F +   T  FMFQ T+TDPNV ++AF+GT+   D  VD++FSW
Sbjct: 181 EGCWKMNLINTYKFRNGFIEKARTCAFMFQTTSTDPNVIILAFKGTSQCCDCAVDVNFSW 240

Query: 254 YEIANVGKIHRGFMEALGLQKQLGWPKELVKPEHDYAYYFLRQQLRDIAKSNDKARFIIT 313
             I  VG+IH GFM+ALGLQ+   WP EL KP  D+AYY LRQQLRDIAKSND ARFI T
Sbjct: 241 CSIPGVGRIHMGFMQALGLQEAFHWPDELPKPNSDFAYYTLRQQLRDIAKSNDNARFIFT 300

Query: 314 GHSLGAALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFPY 373
           GHSLG ALA LFVTILA+H E D+LEKL+AVYTFGQPRVGD +F QFMEN   K + F Y
Sbjct: 301 GHSLGGALAVLFVTILAYHNESDVLEKLQAVYTFGQPRVGDPKFAQFMENTTKK-YDFKY 360

Query: 374 YRYVYSMDVVPRIPFDVEGDQW--YKHFGGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPK 433
           YRYVYS D+V RIPF+  GD +  YKHFGGC+Y+DC Y G+FLEE+PN NY    +LIP+
Sbjct: 361 YRYVYSFDLVSRIPFNC-GDFFCSYKHFGGCVYFDCCYNGKFLEEQPNNNYYWPIYLIPQ 420

Query: 434 YLYAWWELLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASAHNVVNYIYSARWGKI 485
           +L A WE +RSL++P++ C+ DYFE       RV GLFIP  SAH ++NYI S R+GKI
Sbjct: 421 FLAALWEFIRSLMIPLVKCSWDYFEGFGNLKNRVLGLFIPATSAHMLLNYINSTRYGKI 445

[Close]

BLAST of MC09g0056 vs. ExPASy TrEMBL
Match: A0A6J1DGJ7 (uncharacterized protein LOC111020243 OS=Momordica charantia OX=3673 GN=LOC111020243 PE=4 SV=1)

HSP 1 Score: 929 bits (2401), Expect = 0.0
Identity = 452/490 (92.24%), Postives = 453/490 (92.45%), Query Frame = 0


Query: 14  MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII 73
           MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII
Sbjct: 1   MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII 60

Query: 74  FFSILWQKILLAFLNQHKERSGSFGKIIISCFSFSIFLKNLNILQLTFFLLRGHAEKPKT 133
           FFSILWQKILLAFLNQHKE                                     KPKT
Sbjct: 61  FFSILWQKILLAFLNQHKE-------------------------------------KPKT 120

Query: 134 EDPPTVNCKDWSLSGRDKNIKLDDPFQYYAILSVRASALASEDFSPHPSVVETVVNSCWN 193
           EDPPTVNCKDWSLSGRDKNIKLDDPFQYYAILSVRASALA+EDFSPHPSVVETVVNSCWN
Sbjct: 121 EDPPTVNCKDWSLSGRDKNIKLDDPFQYYAILSVRASALATEDFSPHPSVVETVVNSCWN 180

Query: 194 MKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVAFRGTTDVYDMKVDLDFSWYEIAN 253
           MKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVAFRGTTDVYDMKVDLDFSWYEIAN
Sbjct: 181 MKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVAFRGTTDVYDMKVDLDFSWYEIAN 240

Query: 254 VGKIHRGFMEALGLQKQLGWPKELVKPEHDYAYYFLRQQLRDIAKSNDKARFIITGHSLG 313
           VGKIHRGFMEALGLQKQLGWPKELVKPEHDYAYYFLRQQLRDIAKSNDKARFIITGHSLG
Sbjct: 241 VGKIHRGFMEALGLQKQLGWPKELVKPEHDYAYYFLRQQLRDIAKSNDKARFIITGHSLG 300

Query: 314 AALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFPYYRYVY 373
           AALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFPYYRYVY
Sbjct: 301 AALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFPYYRYVY 360

Query: 374 SMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPKYLYAWWE 433
           SMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPKYLYAWWE
Sbjct: 361 SMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPKYLYAWWE 420

Query: 434 LLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASAHNVVNYIYSARWGKIVCRDTQKK 493
           LLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASAHNVVNYIYSARWGKIVCRDTQKK
Sbjct: 421 LLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASAHNVVNYIYSARWGKIVCRDTQKK 453

Query: 494 VIPADYLERC 503
           VIPADYLERC
Sbjct: 481 VIPADYLERC 453

[Close]

BLAST of MC09g0056 vs. ExPASy TrEMBL
Match: A0A6J1CEU2 (uncharacterized protein LOC111010582 OS=Momordica charantia OX=3673 GN=LOC111010582 PE=4 SV=1)

HSP 1 Score: 595 bits (1534), Expect = 1.24e-208
Identity = 296/487 (60.78%), Postives = 348/487 (71.46%), Query Frame = 0


Query: 14  MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII 73
           MGS+E +F+SDYLILKPEEATLL LFLF LPFG++ LR LVDCP  KE SY +FGNRW+I
Sbjct: 1   MGSQENKFSSDYLILKPEEATLLHLFLFTLPFGYRKLRNLVDCPVAKENSYTTFGNRWVI 60

Query: 74  FFSILWQKILLAFLNQHKERSGSFGKIIISCFSFSIFLKNLNILQLTFFLLRGHAEKPKT 133
           F SIL QK LLA  N  K      GK                                KT
Sbjct: 61  FISILLQKFLLAIANLIKYCKSMRGKF------------------------------HKT 120

Query: 134 EDPPTVNCKDWSLSGRDKNIKL--DDPFQYYAILSVRASALASEDF-SPHPSVVETVVNS 193
              P VNCK+W + G +KNIKL  DD F+YY  L+V AS LA +D+ SP PSVV+TVVN 
Sbjct: 121 CAAPKVNCKEWKIIGMEKNIKLADDDTFKYYGALTVMASNLAYQDYNSPEPSVVQTVVNG 180

Query: 194 CWNMKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVAFRGTTDVYDMKVDLDFSWYE 253
           CW M LLGCYD WNDFQ+  +TQVFMFQNTATDPNVTVVAFRG++++YD  VDL+ SWY 
Sbjct: 181 CWKMNLLGCYDFWNDFQNKATTQVFMFQNTATDPNVTVVAFRGSSEIYDWMVDLNLSWYN 240

Query: 254 IANVGKIHRGFMEALGLQKQLGWPKELVKPEHDYAYYFLRQQLRDIAKSNDKARFIITGH 313
           I  +GKIH GFM+ALGLQK  GWPKEL  P+HD+AYY LRQQLRDI KSND ARFI TGH
Sbjct: 241 IEGIGKIHGGFMQALGLQKGTGWPKELANPDHDFAYYTLRQQLRDIVKSNDNARFIFTGH 300

Query: 314 SLGAALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFPYYR 373
           SLG ALA LF T+LAFH++  LLEKL+AVYTFGQPRVGD +F QFMEN   K + F YYR
Sbjct: 301 SLGGALAVLFATLLAFHDDATLLEKLQAVYTFGQPRVGDRRFAQFMENTIEK-YGFKYYR 360

Query: 374 YVYSMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPKYLYA 433
           YVYS D+VPR+PFD   + WY+HFGGC+Y++C+Y G+FLE +PNKNY S  WLI KY+ A
Sbjct: 361 YVYSSDLVPRVPFDFNDNLWYEHFGGCVYFNCSYNGKFLEVQPNKNYFSPIWLISKYVSA 420

Query: 434 WWELLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASAHNVVNYIYSARWGKIVCRDT 493
           WWEL+RSL++PVI C+ DY E       R+FGL  PGASAH  +NYI S RWGK    + 
Sbjct: 421 WWELIRSLMIPVIKCSSDYLEGFSNLLLRLFGLLFPGASAHMCLNYINSIRWGKC---NH 453

Query: 494 QKKVIPA 497
           +K +IPA
Sbjct: 481 EKTLIPA 453

[Close]

BLAST of MC09g0056 vs. ExPASy TrEMBL
Match: A0A6J1C148 (uncharacterized protein LOC111007483 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007483 PE=4 SV=1)

HSP 1 Score: 566 bits (1458), Expect = 6.34e-197
Identity = 296/495 (59.80%), Postives = 358/495 (72.32%), Query Frame = 0


Query: 14  MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII 73
           MG ++ QF++DYLILKPEEATLLDLFLF+LPFG+  LRKLVDCP EKEESY SFGNRW+I
Sbjct: 1   MGPEDYQFSNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEKEESYKSFGNRWVI 60

Query: 74  FFSILWQKILLAFLNQHKERSGSFGKIIISCFSFSIFLKNLNILQLTFFLLRGHAEKPKT 133
           F SIL QK LLA  N  ++     GK                     F+ ++ +A +   
Sbjct: 61  FISILSQKFLLAIANLLEKWEPMRGK---------------------FWRVQTYASE--- 120

Query: 134 EDPPTVNCKDWSLSGRDKNIKL---DDPFQYYAILSVRASALASEDFSP-HPSVVETVVN 193
                VNC+DW + G+++NIKL   DD F YY  ++V AS L  +D SP   SVV TVVN
Sbjct: 121 -----VNCRDWKI-GKEENIKLLNDDDTFVYYGAVTVMASNLVYQDCSPPESSVVATVVN 180

Query: 194 SCWNMKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVAFRGTTDVYDMKVDLDFSWY 253
           SCW MKLLGCYD  N F+    TQ  MFQNTATDPNVTVVAF+GT  +YD  +DL+FSWY
Sbjct: 181 SCWKMKLLGCYDFENMFESKARTQATMFQNTATDPNVTVVAFKGTNTIYDAILDLNFSWY 240

Query: 254 EI-ANVGKIHRGFMEALGLQK-QLGWPKELVKPEHDYAYYFLRQQLRDIAKSNDKARFII 313
            +   +G IH GFMEALGLQK  +GWPKEL K +H +AYY+LRQQLRDIAKSNDKA+FI 
Sbjct: 241 NVDQRIGNIHSGFMEALGLQKGTIGWPKELPKSDHQFAYYYLRQQLRDIAKSNDKAKFIF 300

Query: 314 TGHSLGAALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFP 373
           TGHSLG ALA LFVTIL++H E D+L KL+AVYTFGQPRVG+ QFV+FME+  SK + F 
Sbjct: 301 TGHSLGGALAILFVTILSYHNESDVLNKLQAVYTFGQPRVGNSQFVKFMEST-SKQYAFK 360

Query: 374 YYRYVYSMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPKY 433
           YYRYVYSMD+VPRIPFD+  D+WYKHFGGC+Y++C Y+G+F+E +PNKNY S  WLIPKY
Sbjct: 361 YYRYVYSMDLVPRIPFDIF-DEWYKHFGGCVYFNCCYSGQFMEVQPNKNYFSPLWLIPKY 420

Query: 434 LYAWWELLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASAHNVVNYIYSARWGKIV- 493
           L+A WEL+RSL++P+I  +  YFE  +    R FGL IPGASAH  VNYIY AR GK+  
Sbjct: 421 LFALWELIRSLMMPLIKFSLYYFEGFKTLLLRFFGLLIPGASAHLCVNYIYLARQGKMPP 463

Query: 494 CRDTQKKVIPADYLE 501
            +D QK +I  D LE
Sbjct: 481 LKDIQKTLISEDGLE 463

[Close]

BLAST of MC09g0056 vs. ExPASy TrEMBL
Match: A0A6J1C321 (uncharacterized protein LOC111007483 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007483 PE=4 SV=1)

HSP 1 Score: 557 bits (1435), Expect = 2.03e-193
Identity = 295/495 (59.60%), Postives = 354/495 (71.52%), Query Frame = 0


Query: 14  MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII 73
           MGS E +F++DYL LKP+EATLLDLF+F+LPFG   LRKLVDCPPEKEESY SF NRW+I
Sbjct: 1   MGSLEYEFSNDYLKLKPKEATLLDLFIFLLPFGSIKLRKLVDCPPEKEESYTSFTNRWVI 60

Query: 74  FFSILWQKILLAFLNQHKERSGSFGKIIISCFSFSIFLKNLNILQLTFFLLRGHAEKPKT 133
           FFSIL QK L A  N  ++   S  K                        LR   +   +
Sbjct: 61  FFSILSQKSLFALANLLEKWESSTEK------------------------LRTAPQTYAS 120

Query: 134 EDPPTVNCKDWSLSGRDKNIKL---DDPFQYYAILSVRASALASEDFSP-HPSVVETVVN 193
           E    VNC+DW + G+++NIKL   DD F YY  ++V AS L  +D SP   SVV TVVN
Sbjct: 121 E----VNCRDWKI-GKEENIKLLNDDDTFVYYGAVTVMASNLVYQDCSPPESSVVATVVN 180

Query: 194 SCWNMKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVAFRGTTDVYDMKVDLDFSWY 253
           SCW MKLLGCYD  N F+    TQ  MFQNTATDPNVTVVAF+GT  +YD  +DL+FSWY
Sbjct: 181 SCWKMKLLGCYDFENMFESKARTQATMFQNTATDPNVTVVAFKGTNTIYDAILDLNFSWY 240

Query: 254 EI-ANVGKIHRGFMEALGLQK-QLGWPKELVKPEHDYAYYFLRQQLRDIAKSNDKARFII 313
            +   +G IH GFMEALGLQK  +GWPKEL K +H +AYY+LRQQLRDIAKSNDKA+FI 
Sbjct: 241 NVDQRIGNIHSGFMEALGLQKGTIGWPKELPKSDHQFAYYYLRQQLRDIAKSNDKAKFIF 300

Query: 314 TGHSLGAALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFP 373
           TGHSLG ALA LFVTIL++H E D+L KL+AVYTFGQPRVG+ QFV+FME+  SK + F 
Sbjct: 301 TGHSLGGALAILFVTILSYHNESDVLNKLQAVYTFGQPRVGNSQFVKFMEST-SKQYAFK 360

Query: 374 YYRYVYSMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPKY 433
           YYRYVYSMD+VPRIPFD+  D+WYKHFGGC+Y++C Y+G+F+E +PNKNY S  WLIPKY
Sbjct: 361 YYRYVYSMDLVPRIPFDIF-DEWYKHFGGCVYFNCCYSGQFMEVQPNKNYFSPLWLIPKY 420

Query: 434 LYAWWELLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASAHNVVNYIYSARWGKIV- 493
           L+A WEL+RSL++P+I  +  YFE  +    R FGL IPGASAH  VNYIY AR GK+  
Sbjct: 421 LFALWELIRSLMMPLIKFSLYYFEGFKTLLLRFFGLLIPGASAHLCVNYIYLARQGKMPP 464

Query: 494 CRDTQKKVIPADYLE 501
            +D QK +I  D LE
Sbjct: 481 LKDIQKTLISEDGLE 464

[Close]

BLAST of MC09g0056 vs. ExPASy TrEMBL
Match: A0A6J1BSM4 (uncharacterized protein LOC111005368 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005368 PE=4 SV=1)

HSP 1 Score: 511 bits (1317), Expect = 6.64e-176
Identity = 268/479 (55.95%), Postives = 330/479 (68.89%), Query Frame = 0


Query: 14  MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII 73
           MGS++ QF+ DYLILKPEEATLLDLFLF+LPFGF+ +RKLVDCP  KE SY S  +R II
Sbjct: 1   MGSEDHQFSDDYLILKPEEATLLDLFLFILPFGFRKIRKLVDCPAAKENSYRSIQSRLII 60

Query: 74  FFSILWQKILLAFLNQHKERSGSFGKIIISCFSFSIFLKNLNILQLTFFLLRGH----AE 133
           F SIL+QK++LA  N  K+                            F L+RG     + 
Sbjct: 61  FVSILFQKLVLAISNLAKK----------------------------FKLMRGKLWAVSY 120

Query: 134 KPKTEDPPTVNCKDWSLSGRDKNIKL-DDPFQYYAILSVRASALASEDFSPHPSVVETVV 193
           KP     P VNC+DW + G +KN+KL +D F+YY+ L+V AS LA  D+SP P++V+TVV
Sbjct: 121 KPYA---PKVNCRDWKIGG-EKNVKLPEDNFKYYSALTVMASQLAYTDYSPQPNIVQTVV 180

Query: 194 NSCWNMKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVAFRGTTDVYDMKVDLDFSW 253
             CW M L+  Y   N F +   T  FMFQ T+TDPNV ++AF+GT+   D  VD++FSW
Sbjct: 181 EGCWKMNLINTYKFRNGFIEKARTCAFMFQTTSTDPNVIILAFKGTSQCCDCAVDVNFSW 240

Query: 254 YEIANVGKIHRGFMEALGLQKQLGWPKELVKPEHDYAYYFLRQQLRDIAKSNDKARFIIT 313
             I  VG+IH GFM+ALGLQ+   WP EL KP  D+AYY LRQQLRDIAKSND ARFI T
Sbjct: 241 CSIPGVGRIHMGFMQALGLQEAFHWPDELPKPNSDFAYYTLRQQLRDIAKSNDNARFIFT 300

Query: 314 GHSLGAALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFPY 373
           GHSLG ALA LFVTILA+H E D+LEKL+AVYTFGQPRVGD +F QFMEN   K + F Y
Sbjct: 301 GHSLGGALAVLFVTILAYHNESDVLEKLQAVYTFGQPRVGDPKFAQFMENTTKK-YDFKY 360

Query: 374 YRYVYSMDVVPRIPFDVEGDQW--YKHFGGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPK 433
           YRYVYS D+V RIPF+  GD +  YKHFGGC+Y+DC Y G+FLEE+PN NY    +LIP+
Sbjct: 361 YRYVYSFDLVSRIPFNC-GDFFCSYKHFGGCVYFDCCYNGKFLEEQPNNNYYWPIYLIPQ 420

Query: 434 YLYAWWELLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASAHNVVNYIYSARWGKI 485
           +L A WE +RSL++P++ C+ DYFE       RV GLFIP  SAH ++NYI S R+GKI
Sbjct: 421 FLAALWEFIRSLMIPLVKCSWDYFEGFGNLKNRVLGLFIPATSAHMLLNYINSTRYGKI 445

[Close]

BLAST of MC09g0056 vs. TAIR 10
Match: AT1G45201.1 (triacylglycerol lipase-like 1 )

HSP 1 Score: 326.6 bits (836), Expect = 3.4e-89
Identity = 187/487 (38.40%), Postives = 271/487 (55.65%), Query Frame = 0


Query: 14  MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESY-NSFGNRWI 73
           M    ++F + Y ++ P +A+ LDL L +      + R  +D PP+  + +  SF +RWI
Sbjct: 1   MSKTNMKFCNSYFLVDPTKASFLDLLLLLFSSNLTSAR-FIDSPPDTLKGFRRSFASRWI 60

Query: 74  IFFSILWQKILL------AFLNQHKERSGSFGKIIISCFSFSIFLKNLNILQLTFFLLRG 133
           +  +I  QK+L+      AF+ Q               +  ++   N     L   L+ G
Sbjct: 61  LALAIFLQKVLMLLSKPFAFIGQK------------LTYWLNLLTANGGFFNLILNLMSG 120

Query: 134 HAEKPKTEDPPTVNCKDWSLSGRDKNIKLDD-----PFQYYAILSVRASALASEDFSPHP 193
              KP        +     +   D+ I+LD+       +Y ++LS+ AS ++ E   P+ 
Sbjct: 121 KLVKPDKSSATYTS----FIGCSDRRIELDEKINVGSIEYKSMLSIMASKISYES-KPY- 180

Query: 194 SVVETVVNSCWNMKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVAFRGTT--DVYD 253
             + +VV + W M L+G YD +N FQ+   TQ F+F+ ++T+P++ VV+FRGT   +  D
Sbjct: 181 --ITSVVKNTWKMDLVGNYDFYNAFQESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAAD 240

Query: 254 MKVDLDFSWYEIANVGKIHRGFMEALGLQKQLGWPKELVKPEHDYAYYFLRQQLRDIAKS 313
              DLD SWYE+ NVGK+H GF  ALGLQK  GWPKE +   H YAYY +RQ LRD    
Sbjct: 241 WCTDLDLSWYEMKNVGKVHAGFSRALGLQKD-GWPKENISLLHQYAYYTIRQMLRDKLGR 300

Query: 314 NDKARFIITGHSLGAALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMENV 373
           N   ++I+TGHSLG ALA LF  ILA H E +LL+KL+ +YTFGQPRVGD  F +FM+ V
Sbjct: 301 NKNLKYILTGHSLGGALAALFPAILAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGV 360

Query: 374 NSKVHKFPYYRYVYSMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLEEEPNKNYLS 433
             K H   Y R+VY+ DVVPR+PFD +    YKH+G C  ++  Y G+  E+ PN NY +
Sbjct: 361 VKK-HGIEYERFVYNNDVVPRVPFDDKYLFSYKHYGPCNSFNSLYKGKVREDAPNANYFN 420

Query: 434 IPWLIPKYLYAWWELLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASAHNVVNYIYS 487
           + WLIP+ L   WE +RS +L       +Y E   + + RV G+  PG S H   +Y+ S
Sbjct: 421 LLWLIPQLLTGLWEFIRSFILQ-FWKGDEYKENWLMRFVRVVGIVFPGGSNHFPFDYVNS 463

[Close]

BLAST of MC09g0056 vs. TAIR 10
Match: AT5G42930.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 324.3 bits (830), Expect = 1.7e-88
Identity = 191/468 (40.81%), Postives = 275/468 (58.76%), Query Frame = 0


Query: 24  DYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEK-EESYNSFGNRWIIFFSILWQKI 83
           +Y +L P EAT+ DL   +      + RK +    E+ E+  + F  RWIIF SI+ QK+
Sbjct: 6   NYFVLDPREATVSDLIHLLFSSDLGD-RKFIHSSEERIEDDLSRFRRRWIIFVSIVIQKL 65

Query: 84  LLAFLNQHKERSGSFGKIIISCFSFSIFLKNLNILQLTFFLLRGHAEKPKTEDPPTVNCK 143
           ++ F     ++   F    ++C+  ++   N   L +   L +G+   P+          
Sbjct: 66  IILF-----KKPLYFVGFSLACW-LNLLSSNGGFLMILPNLFKGNIIWPEKASATFA--- 125

Query: 144 DWSLSGR-DKNIKLDDPFQ-----YYAILSVRASALASEDFSPHPSVVETVVNSCWNMKL 203
             SL+G  D+ ++L+   +     Y A+LS+ AS L+ E+     + V +V+++ W M L
Sbjct: 126 --SLNGNLDRRVELNPKVERGSKRYKAMLSIMASKLSYENI----NFVSSVLHNHWKMDL 185

Query: 204 LGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVAFRGTT--DVYDMKVDLDFSWYEIANV 263
           LG Y  WN +Q   ST+V + ++T+TDPN+ +V+FRGT   D  D   DLD SWYE+ NV
Sbjct: 186 LGFYSCWNGYQKQRSTEVIVIKDTSTDPNLIIVSFRGTDPFDADDWCTDLDLSWYEVKNV 245

Query: 264 GKIHRGFMEALGLQKQLGWPKELVKPEHD-----YAYYFLRQQLRDIAKSNDKARFIITG 323
           GKIH GFM+ALGLQK+ GWPKE+   E       YAYY +R+ L++I   N  ++FI+TG
Sbjct: 246 GKIHGGFMKALGLQKE-GWPKEVNLDETQNATTLYAYYTVRRHLKEILDQNPTSKFILTG 305

Query: 324 HSLGAALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMENVNSKVHKFPYY 383
           HSLG ALA LF  +L  H+E  +LE+L+ VYTFGQPRVGD +F  FM++ + K     Y 
Sbjct: 306 HSLGGALAILFTAVLVMHDEEQMLERLEGVYTFGQPRVGDEEFGNFMKD-SLKKFDVKYE 365

Query: 384 RYVYSMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLEEEPNKNYLSIPWLIPKYLY 443
           RYVY  D+VPR+PFD +    +KHFG CLYYD  Y G+  EEEPNKNY ++ W++PK + 
Sbjct: 366 RYVYCNDMVPRLPFD-DKTLMFKHFGACLYYDSFYKGKVEEEEPNKNYFNLVWVLPKIMN 425

Query: 444 AWWELLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASAHNVVNYI 478
           A WEL+RS ++P      ++ E   +   RV  L IPG  AH    YI
Sbjct: 426 ALWELIRSFVMP-YWKGGEFREGWFLRCFRVVALLIPGLPAHFPNEYI 453

[Close]

BLAST of MC09g0056 vs. TAIR 10
Match: AT1G56630.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 295.4 bits (755), Expect = 8.5e-80
Identity = 191/500 (38.20%), Postives = 266/500 (53.20%), Query Frame = 0


Query: 24  DYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEK-EESYNSFGNRWIIFFSILWQKI 83
           +Y +L P EAT+ DL   +     +N RK VD   E  E     F  RWIIF SI+ QK+
Sbjct: 6   NYFVLDPREATVTDLMRLLFYSDLEN-RKFVDTSVENLENRLCEFRGRWIIFVSIVVQKL 65

Query: 84  L-------------LAFLNQHKERSGSFGKIIISCFSFSIFLKNLNILQLTFFLLRGHAE 143
           L             L F       +G F KI ++                TF  + G+ +
Sbjct: 66  LIILRKPLSFLGFALGFWLNLPSSNGGFFKIFLNLVKGRFIWPEKT--SATFASINGNLD 125

Query: 144 KPKTEDPPTVNCKDWSLSGRDKNIKLDDPFQYYAILSVRASALASEDFSPHPSVVETVVN 203
           + K E             G  ++IK+ D  +Y  +LS+ AS LA E    +   + +V+ 
Sbjct: 126 Q-KVE------------LGLGRSIKIGDE-RYKPLLSIMASKLAYE----NEHFIRSVLQ 185

Query: 204 SCWNMKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVAFRGTT--DVYDMKVDLDFS 263
             W M LLG Y   NDF    ST+V + ++T  +PN+ VV+FRGT   +  D   DLD S
Sbjct: 186 DHWQMDLLGFYSCPNDFDQTRSTEVIVIRDTKDNPNLIVVSFRGTDPFNADDWCTDLDLS 245

Query: 264 WYEIANVGKIHRGFMEALGLQKQLGWPKELVKPE-----HDYAYYFLRQQLRDIAKSNDK 323
           W+ + NVGKIH GFM+ALGL K+ GW +E+   +        AYY + +QL+++ + N  
Sbjct: 246 WHNVMNVGKIHGGFMKALGLPKE-GWYEEINSDQTQNKTSQLAYYTILRQLKEVFEQNPT 305

Query: 324 ARFIITGHSLGAALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMENVNSK 383
           ++FI++GHSLG ALA LF  +L  H+E ++LE+L+ VYTFGQPRVGD  F  +M++   K
Sbjct: 306 SKFILSGHSLGGALAILFTAVLIMHDEKEMLERLEGVYTFGQPRVGDEDFGIYMKD-KLK 365

Query: 384 VHKFPYYRYVYSMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLEEEPNKNYLSIPW 443
                Y RYVY  D+VPR+PFD +    +KHFGGCLY D  Y G+  EEEPNKNY +I W
Sbjct: 366 EFDVKYKRYVYCNDMVPRLPFD-DKTLMFKHFGGCLYCDSFYKGKVEEEEPNKNYFNIFW 425

Query: 444 LIPKYLYAWWELLRSLLLPVIMC--NPDYFETSQIFYARVFGLFIPGASAHNVVNYIYSA 501
           +IPK + A WEL+RS    +I C    +Y E   +   R+  L IPG  AH    Y+  A
Sbjct: 426 VIPKIINAVWELIRSF---IISCWQGREYREGWLLTCFRLVALLIPGLPAHFPNEYVNVA 478

[Close]

BLAST of MC09g0056 vs. TAIR 10
Match: AT5G67050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 275.0 bits (702), Expect = 1.2e-73
Identity = 171/483 (35.40%), Postives = 252/483 (52.17%), Query Frame = 0


Query: 14  MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESYNSFGNRWII 73
           M S +      YLIL+PEE    +L   +     +  R +       E   +SF +RW+I
Sbjct: 2   MNSDDDDEPRGYLILRPEELRPWELVRLLFSGDIEKPRSV----DSSETEEHSFRHRWLI 61

Query: 74  FFSILWQKILLAFLNQHKERSGSFGKIIISCFSFSI-FLKNLNILQLTFFLLRGHAEKPK 133
           F S++  K+L  F             ++ S   FS+ FL N +   L    LRG    P+
Sbjct: 62  FVSLVLLKLLRFF--------SKLLALVGSALEFSLNFLSNNSFSGL---FLRGEVVMPQ 121

Query: 134 TED---PPTVNCKDWSLSGRDKNIKLDDPFQYYAILSVRASALASEDFSPHPSVVETVVN 193
                    +   D  +S  D  +  +D  +YYA LS+ AS +A E    + + ++ VV 
Sbjct: 122 RTSENYQSFIGHLDTRVS-LDMTLNREDGEKYYAALSIMASKIAYE----NAARIKHVVE 181

Query: 194 SCWNMKLLGCYDLWNDFQDMGSTQVFMFQNTAT------DPNVTVVAFRGTT--DVYDMK 253
           + WNMK LG  D WN++Q+  +TQ F+     T           VVAFRGT   +  D  
Sbjct: 182 NHWNMKYLGLVDYWNEYQEKETTQAFIMSTDETTTRSNGQETTVVVAFRGTELFNSEDWC 241

Query: 254 VDLDFSWYEIANVGKIHRGFMEALGLQKQLGWPKE-LVKPEH--DYAYYFLRQQLRDIAK 313
            D D +W+E+ N+G IH GFM+ALGLQ    WPKE L  P+     AYY +R  L+ +  
Sbjct: 242 SDFDITWFELPNIGNIHGGFMKALGLQNNCSWPKEPLSNPDRKSPLAYYSIRDSLKTLIA 301

Query: 314 SNDKARFIITGHSLGAALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMEN 373
            N   +F++TGHSLG ALA LF  +L  H E +LLE+++ VYT+GQPRVGD +F +FME 
Sbjct: 302 QNKNTKFVLTGHSLGGALAILFTAVLVIHHETELLERIQGVYTYGQPRVGDSKFGEFMEK 361

Query: 374 VNSKVHKFPYYRYVYSMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGEFLEEEPNKNYL 433
              K +   YYR+VY+ D+VPR+P+D + D  +KHFG C+YYD NY  + + E+ ++N+ 
Sbjct: 362 KLEK-YNIKYYRFVYNNDIVPRLPYD-DKDLMFKHFGTCIYYDQNYQAKVMREQSDENFF 421

Query: 434 SIPWLIPKYLYAWWELLRSLLLPVIMCNPDYFETSQIFYARVFGLFIPGASAHNVVNYIY 482
            +  +I     A  E +RS  + V     +Y E   +   R  G+ +PG S H   +Y+ 
Sbjct: 422 LLRGIIKMMWSAILEFIRSFTI-VAEKGSEYSEGWLLKGGRALGIIVPGVSNHTPQDYVN 461

[Close]

BLAST of MC09g0056 vs. TAIR 10
Match: AT1G45201.2 (triacylglycerol lipase-like 1 )

HSP 1 Score: 273.5 bits (698), Expect = 3.4e-73
Identity = 158/408 (38.73%), Postives = 229/408 (56.13%), Query Frame = 0


Query: 14  MGSKEIQFASDYLILKPEEATLLDLFLFVLPFGFKNLRKLVDCPPEKEESY-NSFGNRWI 73
           M    ++F + Y ++ P +A+ LDL L +      + R  +D PP+  + +  SF +RWI
Sbjct: 1   MSKTNMKFCNSYFLVDPTKASFLDLLLLLFSSNLTSAR-FIDSPPDTLKGFRRSFASRWI 60

Query: 74  IFFSILWQKILL------AFLNQHKERSGSFGKIIISCFSFSIFLKNLNILQLTFFLLRG 133
           +  +I  QK+L+      AF+ Q               +  ++   N     L   L+ G
Sbjct: 61  LALAIFLQKVLMLLSKPFAFIGQK------------LTYWLNLLTANGGFFNLILNLMSG 120

Query: 134 HAEKPKTEDPPTVNCKDWSLSGRDKNIKLDD-----PFQYYAILSVRASALASEDFSPHP 193
              KP        +     +   D+ I+LD+       +Y ++LS+ AS ++ E   P+ 
Sbjct: 121 KLVKPDKSSATYTS----FIGCSDRRIELDEKINVGSIEYKSMLSIMASKISYES-KPY- 180

Query: 194 SVVETVVNSCWNMKLLGCYDLWNDFQDMGSTQVFMFQNTATDPNVTVVAFRGTT--DVYD 253
             + +VV + W M L+G YD +N FQ+   TQ F+F+ ++T+P++ VV+FRGT   +  D
Sbjct: 181 --ITSVVKNTWKMDLVGNYDFYNAFQESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAAD 240

Query: 254 MKVDLDFSWYEIANVGKIHRGFMEALGLQKQLGWPKELVKPEHDYAYYFLRQQLRDIAKS 313
              DLD SWYE+ NVGK+H GF  ALGLQK  GWPKE +   H YAYY +RQ LRD    
Sbjct: 241 WCTDLDLSWYEMKNVGKVHAGFSRALGLQKD-GWPKENISLLHQYAYYTIRQMLRDKLGR 300

Query: 314 NDKARFIITGHSLGAALATLFVTILAFHEELDLLEKLKAVYTFGQPRVGDVQFVQFMENV 373
           N   ++I+TGHSLG ALA LF  ILA H E +LL+KL+ +YTFGQPRVGD  F +FM+ V
Sbjct: 301 NKNLKYILTGHSLGGALAALFPAILAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGV 360

Query: 374 NSKVHKFPYYRYVYSMDVVPRIPFDVEGDQWYKHFGGCLYYDCNYTGE 408
             K H   Y R+VY+ DVVPR+PFD +    YKH+G C  ++  Y G+
Sbjct: 361 VKK-HGIEYERFVYNNDVVPRVPFDDKYLFSYKHYGPCNSFNSLYKGK 385

The following BLAST results are available for this feature:

BLAST of MC09g0056 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JFU8	2.8e-67	34.39	Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1	[more]
A0A1S3ZP85	1.4e-66	34.93	Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1	[more]
Q5VKJ7	2.3e-58	31.90	Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1	[more]
O59952	1.1e-07	25.21	Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1	[more]
P19515	1.4e-07	30.13	Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2	[more]

BLAST of MC09g0056 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152547.1	0.0	92.24	uncharacterized protein LOC111020243 [Momordica charantia]	[more]
XP_022139747.1	2.56e-208	60.78	uncharacterized protein LOC111010582 [Momordica charantia]	[more]
XP_022135571.1	1.31e-196	59.80	uncharacterized protein LOC111007483 isoform X2 [Momordica charantia]	[more]
XP_022135562.1	4.19e-193	59.60	uncharacterized protein LOC111007483 isoform X1 [Momordica charantia]	[more]
XP_022132571.1	1.37e-175	55.95	uncharacterized protein LOC111005368 isoform X1 [Momordica charantia]	[more]

BLAST of MC09g0056 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DGJ7	0.0	92.24	uncharacterized protein LOC111020243 OS=Momordica charantia OX=3673 GN=LOC111020...	[more]
A0A6J1CEU2	1.24e-208	60.78	uncharacterized protein LOC111010582 OS=Momordica charantia OX=3673 GN=LOC111010...	[more]
A0A6J1C148	6.34e-197	59.80	uncharacterized protein LOC111007483 isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1C321	2.03e-193	59.60	uncharacterized protein LOC111007483 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1BSM4	6.64e-176	55.95	uncharacterized protein LOC111005368 isoform X1 OS=Momordica charantia OX=3673 G...	[more]

BLAST of MC09g0056 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G45201.1	3.4e-89	38.40	triacylglycerol lipase-like 1	[more]
AT5G42930.1	1.7e-88	40.81	alpha/beta-Hydrolases superfamily protein	[more]
AT1G56630.1	8.5e-80	38.20	alpha/beta-Hydrolases superfamily protein	[more]
AT5G67050.1	1.2e-73	35.40	alpha/beta-Hydrolases superfamily protein	[more]
AT1G45201.2	3.4e-73	38.73	triacylglycerol lipase-like 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 159..420 e-value: 6.1E-44 score: 152.2
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 203..414
IPR002921	Fungal lipase-like domain	PFAM	PF01764	Lipase_3	coord: 228..384 e-value: 1.3E-35 score: 122.4
IPR044819	Triacylglycerol lipase OBL1-like	PANTHER	PTHR46086	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 18..488
None	No IPR available	PANTHER	PTHR46086:SF4	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 18..488
None	No IPR available	CDD	cd00519	Lipase_3	coord: 200..407 e-value: 4.31603E-40 score: 142.615

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC09g0056.1	MC09g0056.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016020	membrane
molecular_function	GO:0004806	triglyceride lipase activity