MC08g_new0623 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g_new0623
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	oviduct-specific glycoprotein-like
Location	MC08: 32786471 .. 32787346 (-)
RNA-Seq Expression	MC08g_new0623
Synteny	MC08g_new0623

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCAATCCTGATGTGAAGACATTGTTGTCCATTCCAGGAGACAGAGACGTATTTACTAAAATGGCAAGCGAACCCACATATCATGAAGCATTTATTCAATCCTCCATTGAAGTAGCAGCAAAAGGCAAGTTCAACGATTTGGATCTGCAGTGGCTGTACCCATCTTCTCAAGAAGAAATGCAAAACTTTGAAATTCTCCTTGGTGCATGGCGCCAAGCTGCTGTGGATGATGCTAAAACCTACCATCGACCACAATGGATCTTGGTTGCTTCGGTCTCCAACTTACCCTATGTTGAGAGTAGTATCGTGTACCTAGTTCAAGGAATCAAACAAAACTTGGATTGGGTAAACCTCATTTCTTACGACTTTTACACCCCAACTTCCTTCTCCTCTACTACTGGACCATCCTCTGCACTCCACAACCGCATAGCCAGATCATTGTCCGCAAATTTTGGAATTGACGCTTGGATTCAAAGCCTTGCCAATTTGCCTCGTAAGCAGATTGTATTCGAAATCCCTTTCCACGGTTGGGCATGGAAGCTCGCCAACCCTGACAAGAACGACGTCTTTTCAAAGGCTCTTGGCCCAGCTAAAGGATATCAATATATTTCTCAAGACGGCCAAATCGATTACTCAAATGTCAGAAAGTTTATAAAAGAGAACCATGCTGAGTATGTGAAAGATGACTCCCGATATTCAATCTCCTACGCCTATGCTGGCAGCACTTGGATTGCATACGAAAGTGAATACATCATTCCGGATAAAATTTCATCTGCAAAGTCAAGCAAGGTGCTTGGTTACTTTGCTTGGAACATTGCTGCTGACAACGAAGTCAACACTCTTGCTAGCGCAGCCCAAACCAATTGGTCCTAA

mRNA sequence

ATGAGCAATCCTGATGTGAAGACATTGTTGTCCATTCCAGGAGACAGAGACGTATTTACTAAAATGGCAAGCGAACCCACATATCATGAAGCATTTATTCAATCCTCCATTGAAGTAGCAGCAAAAGGCAAGTTCAACGATTTGGATCTGCAGTGGCTGTACCCATCTTCTCAAGAAGAAATGCAAAACTTTGAAATTCTCCTTGGTGCATGGCGCCAAGCTGCTGTGGATGATGCTAAAACCTACCATCGACCACAATGGATCTTGGTTGCTTCGGTCTCCAACTTACCCTATGTTGAGAGTAGTATCGTGTACCTAGTTCAAGGAATCAAACAAAACTTGGATTGGGTAAACCTCATTTCTTACGACTTTTACACCCCAACTTCCTTCTCCTCTACTACTGGACCATCCTCTGCACTCCACAACCGCATAGCCAGATCATTGTCCGCAAATTTTGGAATTGACGCTTGGATTCAAAGCCTTGCCAATTTGCCTCGTAAGCAGATTGTATTCGAAATCCCTTTCCACGGTTGGGCATGGAAGCTCGCCAACCCTGACAAGAACGACGTCTTTTCAAAGGCTCTTGGCCCAGCTAAAGGATATCAATATATTTCTCAAGACGGCCAAATCGATTACTCAAATGTCAGAAAGTTTATAAAAGAGAACCATGCTGAGTATGTGAAAGATGACTCCCGATATTCAATCTCCTACGCCTATGCTGGCAGCACTTGGATTGCATACGAAAGTGAATACATCATTCCGGATAAAATTTCATCTGCAAAGTCAAGCAAGGTGCTTGGTTACTTTGCTTGGAACATTGCTGCTGACAACGAAGTCAACACTCTTGCTAGCGCAGCCCAAACCAATTGGTCCTAA

Coding sequence (CDS)

ATGAGCAATCCTGATGTGAAGACATTGTTGTCCATTCCAGGAGACAGAGACGTATTTACTAAAATGGCAAGCGAACCCACATATCATGAAGCATTTATTCAATCCTCCATTGAAGTAGCAGCAAAAGGCAAGTTCAACGATTTGGATCTGCAGTGGCTGTACCCATCTTCTCAAGAAGAAATGCAAAACTTTGAAATTCTCCTTGGTGCATGGCGCCAAGCTGCTGTGGATGATGCTAAAACCTACCATCGACCACAATGGATCTTGGTTGCTTCGGTCTCCAACTTACCCTATGTTGAGAGTAGTATCGTGTACCTAGTTCAAGGAATCAAACAAAACTTGGATTGGGTAAACCTCATTTCTTACGACTTTTACACCCCAACTTCCTTCTCCTCTACTACTGGACCATCCTCTGCACTCCACAACCGCATAGCCAGATCATTGTCCGCAAATTTTGGAATTGACGCTTGGATTCAAAGCCTTGCCAATTTGCCTCGTAAGCAGATTGTATTCGAAATCCCTTTCCACGGTTGGGCATGGAAGCTCGCCAACCCTGACAAGAACGACGTCTTTTCAAAGGCTCTTGGCCCAGCTAAAGGATATCAATATATTTCTCAAGACGGCCAAATCGATTACTCAAATGTCAGAAAGTTTATAAAAGAGAACCATGCTGAGTATGTGAAAGATGACTCCCGATATTCAATCTCCTACGCCTATGCTGGCAGCACTTGGATTGCATACGAAAGTGAATACATCATTCCGGATAAAATTTCATCTGCAAAGTCAAGCAAGGTGCTTGGTTACTTTGCTTGGAACATTGCTGCTGACAACGAAGTCAACACTCTTGCTAGCGCAGCCCAAACCAATTGGTCCTAA

Protein sequence

MSNPDVKTLLSIPGDRDVFTKMASEPTYHEAFIQSSIEVAAKGKFNDLDLQWLYPSSQEEMQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYLVQGIKQNLDWVNLISYDFYTPTSFSSTTGPSSALHNRIARSLSANFGIDAWIQSLANLPRKQIVFEIPFHGWAWKLANPDKNDVFSKALGPAKGYQYISQDGQIDYSNVRKFIKENHAEYVKDDSRYSISYAYAGSTWIAYESEYIIPDKISSAKSSKVLGYFAWNIAADNEVNTLASAAQTNWS

Homology

[Close]

BLAST of MC08g_new0623 vs. ExPASy Swiss-Prot
Match: O81862 (Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 3.8e-53
Identity = 123/293 (41.98%), Postives = 168/293 (57.34%), Query Frame = 0


Query: 3   NPDVKTLLSIPG---DRDVFTKMASEPTYHEAFIQSSIEVAAKGKFNDLDLQWLYPSSQE 62
           NP VKTLLSI G   D+  +  MAS PT  ++FI SSI VA    F+ LDL W YPSS  
Sbjct: 87  NPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPSSAT 146

Query: 63  EMQNFEILLGAWRQAAVDDAKTYHRPQWILVASV--SNLPYVESSIVYLVQGIKQNLDWV 122
           EM NF  LL  WR A V +A +  +P+ +L A+V  SN  Y   S++Y V  +  +LDWV
Sbjct: 147 EMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYY---SVLYPVSAVASSLDWV 206

Query: 123 NLISYDFYTPTSFSSTTGPSSALHNRIARSLSANFGIDAWIQSLANLPRKQIVFEIPFHG 182
           NL++YDFY P  +S  TGP +AL +      S + G  +WIQ  A LP K+ V   P++G
Sbjct: 207 NLMAYDFYGP-GWSRVTGPPAALFDPSNAGPSGDAGTRSWIQ--AGLPAKKAVLGFPYYG 266

Query: 183 WAWKLANPDKNDVFSKALGPAKGYQYISQDGQIDYSNVRKFIKENHAEYVKDDSRYSISY 242
           +AW+L N + +  ++   G A     IS DG I Y  +RKFI +N A  V + +     Y
Sbjct: 267 YAWRLTNANSHSYYAPTTGAA-----ISPDGSIGYGQIRKFIVDNGATTVYNSTVVG-DY 326

Query: 243 AYAGSTWIAYESEYIIPDKISSAKSSKVLGYFAWNIAADNEVNTLASAAQTNW 291
            YAG+ WI Y+    I  K+  AK   +LGYF+W++ AD+  + L+ AA   W
Sbjct: 327 CYAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADDN-SGLSRAASQAW 366

[Close]

BLAST of MC08g_new0623 vs. ExPASy Swiss-Prot
Match: A0A1B1J8Z2 (Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2)

HSP 1 Score: 163.7 bits (413), Expect = 3.1e-39
Identity = 101/298 (33.89%), Postives = 169/298 (56.71%), Query Frame = 0


Query: 4   PDVKTLLSIPG---DRDVFTKMASEPTYHEAFIQSSIEVAAKGKFNDLDLQWLYPSSQEE 63
           P V TLLSI G   +  +F++MAS      +FI S+I VA K +FN LDL W +P  +++
Sbjct: 114 PRVTTLLSIGGGGNNATLFSEMASSKQNRASFINSTIHVARKHEFNGLDLDWEWPGDEKD 173

Query: 64  MQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSI--------VYLVQGIKQ 123
           M N  +LL  W +A V +A T  + + +L ++V    Y  S+I         Y V+ I++
Sbjct: 174 MSNLALLLKEWYKALVVEANTSRKSRLLLTSAV----YFNSTISLIGNGPRSYPVRAIRK 233

Query: 124 NLDWVNLISYDFYTPTSFSSTTGPSSALHNRIARSLSANFGIDAWIQSLANLPRKQIVFE 183
            LDW + + +D+    ++++ TG ++AL++  + ++S  +GI +WI S   +P +++V  
Sbjct: 234 YLDWASPMCFDY--NGAWANETGFNAALYDPNS-NISTKYGIGSWIGS--GVPAEKLVMG 293

Query: 184 IPFHGWAWKLANPDKNDVFSKALGPAKGYQYISQDGQIDYSNVRKFIKENHAEYVKDDSR 243
           +P +G AW+L +P+ + V +KA+GPA     +  DG +DY  +  F K+  A+ V D+  
Sbjct: 294 LPLYGRAWELKDPNDHGVGAKAVGPA-----VDTDGSMDYDEILVFNKDTGAKVVYDEVA 353

Query: 244 YSISYAYAGSTWIAYESEYIIPDKISSAKSSKVLGYFAWNIAADNEVNTLASAAQTNW 291
            S  Y+Y+G+TWI Y+    I  K+  A+S  + GYF W I  D +  T++  A   W
Sbjct: 354 VSF-YSYSGTTWIGYDDGPSITKKVQFARSMGLKGYFFWAIGKDKDW-TISKQASNAW 395

[Close]

BLAST of MC08g_new0623 vs. ExPASy Swiss-Prot
Match: A0A072VEP0 (Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 2.9e-37
Identity = 98/300 (32.67%), Postives = 166/300 (55.33%), Query Frame = 0


Query: 3   NPDVKTLLSIPG---DRDVFTKMASEPTYHEAFIQSSIEVAAKGKFNDLDLQWLYPSSQE 62
           +P VKTLLSI G   +  +F+++AS     E FI S+IEVA K +F+ +DL W +P +Q+
Sbjct: 99  SPPVKTLLSIGGGGSNSTLFSEIASTKQNREIFINSTIEVARKYRFDGVDLDWEFPETQQ 158

Query: 63  EMQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSI---------VYLVQGI 122
           +M N  +L   W  A   +AK   +P+ +L ++V    Y  S++          Y  Q I
Sbjct: 159 DMFNLGLLYEEWYNALFAEAKVRRKPRLLLTSAV----YYNSTVRLIGKHGPRSYPTQAI 218

Query: 123 KQNLDWVNLISYDFYTPTSFSSTTGPSSALHNRIARSLSANFGIDAWIQSLANLPRKQIV 182
            + LDW + + +D++   ++ + T  ++AL++     +S NFG+ +WI+S   +  +++V
Sbjct: 219 NKYLDWASPMCFDYH--GTWDNNTDFNAALYDS-KSEISTNFGLHSWIKS--GVRPEKLV 278

Query: 183 FEIPFHGWAWKLANPDKNDVFSKALGPAKGYQYISQDGQIDYSNVRKFIKENHAEYVKDD 242
             +  +G AW+L +P+ N V ++A+GPA        DG ++Y+ + KF K++ A  V D 
Sbjct: 279 MGLALYGRAWELKDPNVNGVGAEAVGPA-----TDTDGSMNYNEILKFNKQSGANVVYDK 338

Query: 243 SRYSISYAYAGSTWIAYESEYIIPDKISSAKSSKVLGYFAWNIAADNEVNTLASAAQTNW 291
              S  Y+YAG+TWI Y+    I  K+  AKS  + GYF W +  D +  +++  A   W
Sbjct: 339 VAISF-YSYAGTTWIGYDDGPSITTKVRFAKSLGLKGYFFWALGKDKDW-SISKQASNAW 382

[Close]

BLAST of MC08g_new0623 vs. ExPASy Swiss-Prot
Match: U5N4E3 (Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2)

HSP 1 Score: 147.5 bits (371), Expect = 2.3e-34
Identity = 95/290 (32.76%), Postives = 156/290 (53.79%), Query Frame = 0


Query: 4   PDVKTLLSIPG---DRDVFTKMASEPTYHEAFIQSSIEVAAKGKFNDLDLQWLYPSSQEE 63
           P + TLLSI G   +  +F  +AS+PT    FI S+I+VA    F+ +D  W +P++ +E
Sbjct: 101 PPIPTLLSIGGATSNSTLFAFIASDPTARATFINSTIQVARTFGFDGIDFDWEFPTTTKE 160

Query: 64  MQNFEILLGAWRQAAVDDAKTYHRPQWILVASV---SNLPYVESSIVYLVQGIKQNLDWV 123
           M +   LL  WR+A  D+  +  RP  +L A+V    N        +Y V  I +NLDWV
Sbjct: 161 MNDLGELLFQWRRAISDETASTSRPPLLLTAAVYFAVNFFLSGERRMYPVDSINKNLDWV 220

Query: 124 NLISYDFYTPTSFSSTTGPSSALHNRIARSLSANFGIDAWIQSLANLPRKQIVFEIPFHG 183
           N++SYD     S S+ TG  S + +    ++S   G+ +WI+    +  ++IV  +P +G
Sbjct: 221 NVMSYDL--RGSGSNVTGAPSGMFDS-KSNVSVVSGLFSWIR--GGVAPEKIVMGMPLYG 280

Query: 184 WAWKLANPDKNDVFSKALGPAKGYQYISQDGQIDYSNVRKFIKENHAEYVKDDSRYSISY 243
            +WKL +P+ + + +  +GP  G      DG + Y  V  F K+  A+ V D    S+ Y
Sbjct: 281 KSWKLQDPNVHGIGAPNVGPGPGV-----DGGMAYFQVVDFNKQMGAKVVYDKETGSV-Y 340

Query: 244 AYAGSTWIAYESEYIIPDKISSAKSSKVLGYFAWNIAADNEVNTLASAAQ 288
           +Y+GSTWI Y+  + +  K+  A++ K+ GYF W    D     +++A++
Sbjct: 341 SYSGSTWIGYDDPFTVSVKVGFAQALKLGGYFFWAAGYDTSDWKVSTASK 379

[Close]

BLAST of MC08g_new0623 vs. ExPASy Swiss-Prot
Match: A0A072UR65 (Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 9.8e-33
Identity = 91/294 (30.95%), Postives = 152/294 (51.70%), Query Frame = 0


Query: 3   NPDVKTLLSIPG---DRDVFTKMASEPTYHEAFIQSSIEVAAKGKFNDLDLQWLYPSSQE 62
           +P   TL SI G   +  +   +AS+P    AFI S+I+VA    F+ +DL W +P + +
Sbjct: 94  SPPAATLFSIGGAGSNSSLLAFIASDPPACAAFINSTIDVARTFGFDGIDLDWEFPKNTK 153

Query: 63  EMQNFEILLGAWRQAAVDDAKTYHRPQWILVASV---SNLPYVESSIVYLVQGIKQNLDW 122
           EM +   +L  WR+A  D+  T  RP  +L A+V    N        +Y V  I +NLDW
Sbjct: 154 EMNDLGEMLFQWRKAISDEGATTGRPPLLLTAAVYFAVNFSIYGEPRMYPVNSINENLDW 213

Query: 123 VNLISYDFYTPTSFSSTTGPSSALHNRIARSLSANFGIDAWIQSLANLPRKQIVFEIPFH 182
           VN++SY+   P S + T  PS     +   ++S   G+ +WI S   +  +++V  +P +
Sbjct: 214 VNVMSYELRGPRS-NKTGAPSGTFDPK--SNVSVVSGLLSWIHS--GVVPEKLVMGMPLY 273

Query: 183 GWAWKLANPDKNDVFSKALGPAKGYQYISQDGQIDYSNVRKFIKENHAEYVKDDSRYSIS 242
           G +WKL +P+ + + + ++G   G      +G + Y  V  F ++  A+   D    S+ 
Sbjct: 274 GKSWKLRDPNVHGIGAPSVGSGPGV-----NGLMAYFQVLDFNRQKSAKVEYDVDTASV- 333

Query: 243 YAYAGSTWIAYESEYIIPDKISSAKSSKVLGYFAWNIAADNEVNTLASAAQTNW 291
           Y+Y+GSTWI Y++ + +  K+  A++ K+ GYF W    D     +A+ A   W
Sbjct: 334 YSYSGSTWIGYDNPFTVSIKVGFAQALKLRGYFFWVAGLDTLDWKIATQASKAW 376

[Close]

BLAST of MC08g_new0623 vs. NCBI nr
Match: XP_022142964.1 (oviduct-specific glycoprotein-like [Momordica charantia])

HSP 1 Score: 592 bits (1526), Expect = 1.71e-213
Identity = 291/291 (100.00%), Postives = 291/291 (100.00%), Query Frame = 0


Query: 1   MSNPDVKTLLSIPGDRDVFTKMASEPTYHEAFIQSSIEVAAKGKFNDLDLQWLYPSSQEE 60
           MSNPDVKTLLSIPGDRDVFTKMASEPTYHEAFIQSSIEVAAKGKFNDLDLQWLYPSSQEE
Sbjct: 1   MSNPDVKTLLSIPGDRDVFTKMASEPTYHEAFIQSSIEVAAKGKFNDLDLQWLYPSSQEE 60

Query: 61  MQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYLVQGIKQNLDWVNLI 120
           MQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYLVQGIKQNLDWVNLI
Sbjct: 61  MQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYLVQGIKQNLDWVNLI 120

Query: 121 SYDFYTPTSFSSTTGPSSALHNRIARSLSANFGIDAWIQSLANLPRKQIVFEIPFHGWAW 180
           SYDFYTPTSFSSTTGPSSALHNRIARSLSANFGIDAWIQSLANLPRKQIVFEIPFHGWAW
Sbjct: 121 SYDFYTPTSFSSTTGPSSALHNRIARSLSANFGIDAWIQSLANLPRKQIVFEIPFHGWAW 180

Query: 181 KLANPDKNDVFSKALGPAKGYQYISQDGQIDYSNVRKFIKENHAEYVKDDSRYSISYAYA 240
           KLANPDKNDVFSKALGPAKGYQYISQDGQIDYSNVRKFIKENHAEYVKDDSRYSISYAYA
Sbjct: 181 KLANPDKNDVFSKALGPAKGYQYISQDGQIDYSNVRKFIKENHAEYVKDDSRYSISYAYA 240

Query: 241 GSTWIAYESEYIIPDKISSAKSSKVLGYFAWNIAADNEVNTLASAAQTNWS 291
           GSTWIAYESEYIIPDKISSAKSSKVLGYFAWNIAADNEVNTLASAAQTNWS
Sbjct: 241 GSTWIAYESEYIIPDKISSAKSSKVLGYFAWNIAADNEVNTLASAAQTNWS 291

[Close]

BLAST of MC08g_new0623 vs. NCBI nr
Match: XP_022159350.1 (chitinase-3-like protein 2 [Momordica charantia])

HSP 1 Score: 536 bits (1380), Expect = 3.84e-190
Identity = 263/291 (90.38%), Postives = 276/291 (94.85%), Query Frame = 0


Query: 1   MSNPDVKTLLSIPGDRDVFTKMASEPTYHEAFIQSSIEVAAKGKFNDLDLQWLYPSSQEE 60
           MSNPDVKTLLSI GDRDVF KMASEPTY + FIQSSIEVAAKGKFN LDLQWLYPSS+EE
Sbjct: 70  MSNPDVKTLLSIRGDRDVFAKMASEPTYRDGFIQSSIEVAAKGKFNGLDLQWLYPSSEEE 129

Query: 61  MQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYLVQGIKQNLDWVNLI 120
           MQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVY V+ IKQNLDWVNLI
Sbjct: 130 MQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYPVEEIKQNLDWVNLI 189

Query: 121 SYDFYTPTSFSSTTGPSSALHNRIARSLSANFGIDAWIQSLANLPRKQIVFEIPFHGWAW 180
           SYDFYTP S  S TGPSSALHNR+ARSLSANFGIDAWIQSLANLPRKQIVF IPFHGWAW
Sbjct: 190 SYDFYTPASSPSATGPSSALHNRMARSLSANFGIDAWIQSLANLPRKQIVFGIPFHGWAW 249

Query: 181 KLANPDKNDVFSKALGPAKGYQYISQDGQIDYSNVRKFIKENHAEYVKDDSRYSISYAYA 240
           KLANPDK+DVFSKALGPAKG QYISQ+GQID SNV++FIK+NHA+YVKDDSRYSISYAYA
Sbjct: 250 KLANPDKHDVFSKALGPAKGDQYISQNGQIDXSNVKRFIKQNHAQYVKDDSRYSISYAYA 309

Query: 241 GSTWIAYESEYIIPDKISSAKSSKVLGYFAWNIAADNEVNTLASAAQTNWS 291
           G+TWIAYESEY IPDKISSAKSSKVLGYFAWNIAAD+EVNTLA+AAQTNWS
Sbjct: 310 GTTWIAYESEYTIPDKISSAKSSKVLGYFAWNIAADDEVNTLANAAQTNWS 360

[Close]

BLAST of MC08g_new0623 vs. NCBI nr
Match: XP_022999577.1 (chitotriosidase-1-like [Cucurbita maxima])

HSP 1 Score: 350 bits (897), Expect = 9.88e-117
Identity = 174/290 (60.00%), Postives = 218/290 (75.17%), Query Frame = 0


Query: 1   MSNPDVKTLLSIPGDRDVFTKMASEPTYHEAFIQSSIEVAAKGKFNDLDLQWLYPSSQEE 60
           +SNPDVKTLLSI GD  VF  MA++PT  +AFI+SSI  A  G F+ LDLQWLYPSSQ++
Sbjct: 70  LSNPDVKTLLSIAGDAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQDQ 129

Query: 61  MQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYLVQGIKQNLDWVNLI 120
           M NF+ L+ +W QA VDDA +   PQ +LVA+VSNLPY++++I Y    I Q LDWVNL+
Sbjct: 130 MANFKTLIHSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAITQKLDWVNLL 189

Query: 121 SYDFYTPTSFSSTTGPSSALHNRIARSLSANFGIDAWIQSLANLPRKQIVFEIPFHGWAW 180
           SYDFYTP SF + TGPSSA HN  A +LSANFGI +W QSL NL  K+IVF IPFHGWAW
Sbjct: 190 SYDFYTPDSFRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLSHKKIVFGIPFHGWAW 249

Query: 181 KLANPDKNDVFSKALGPAKGYQYISQDGQIDYSNVRKFIKENHAEYVKDDSRYSISYAYA 240
           KLAN  ++ +F+KA G A G +YI   GQI YS+V+KFI++N A     D  Y ++YAY 
Sbjct: 250 KLANSHQHALFAKADGAASG-EYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYAYY 309

Query: 241 GSTWIAYESEYIIPDKISSAKSSKVLGYFAWNIAADNEVNTLASAAQTNW 290
            +TWI +ESEY + DKI+ AK+ KVLGYFA+NIAAD+  NTLA+AA++NW
Sbjct: 310 ETTWITFESEYTVADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 358

[Close]

BLAST of MC08g_new0623 vs. NCBI nr
Match: KAG7030350.1 (Chitotriosidase-1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 348 bits (892), Expect = 5.66e-116
Identity = 173/290 (59.66%), Postives = 217/290 (74.83%), Query Frame = 0


Query: 1   MSNPDVKTLLSIPGDRDVFTKMASEPTYHEAFIQSSIEVAAKGKFNDLDLQWLYPSSQEE 60
           +SNP+VKTLLSI  D  +F  MA++PT  +AFI+SSI  A  G F+ LDLQWLYPSSQ++
Sbjct: 70  LSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQDQ 129

Query: 61  MQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYLVQGIKQNLDWVNLI 120
           M NF+ L+ +W QA VDDA +   PQ +LVA+VSNLPY++S+I Y    I Q LDWVNL+
Sbjct: 130 MDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWVNLL 189

Query: 121 SYDFYTPTSFSSTTGPSSALHNRIARSLSANFGIDAWIQSLANLPRKQIVFEIPFHGWAW 180
           SYDFYTP S  + TGPSSA HN  A +LSANFGI +W+QSL NLP K+IVF IPFHGWAW
Sbjct: 190 SYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKKIVFGIPFHGWAW 249

Query: 181 KLANPDKNDVFSKALGPAKGYQYISQDGQIDYSNVRKFIKENHAEYVKDDSRYSISYAYA 240
           KL N  ++ +F+KA G A G +YI   GQI YS+V+KFI++N A     D  Y ++YAY 
Sbjct: 250 KLFNSHQHTLFAKADGAATG-EYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYAYY 309

Query: 241 GSTWIAYESEYIIPDKISSAKSSKVLGYFAWNIAADNEVNTLASAAQTNW 290
            +TWI YESEY I DKI+ AK+ KVLGYFA+NIAAD+  NTLA+AA++NW
Sbjct: 310 ETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

[Close]

BLAST of MC08g_new0623 vs. NCBI nr
Match: XP_022999580.1 (chitotriosidase-1-like [Cucurbita maxima])

HSP 1 Score: 348 bits (892), Expect = 5.66e-116
Identity = 174/290 (60.00%), Postives = 216/290 (74.48%), Query Frame = 0


Query: 1   MSNPDVKTLLSIPGDRDVFTKMASEPTYHEAFIQSSIEVAAKGKFNDLDLQWLYPSSQEE 60
           +SNP VKTLLSI GD  VF  MA +PT  +AFI+SSI  A  G F+ LDLQWLYPSSQ++
Sbjct: 70  LSNPGVKTLLSIAGDAAVFAAMAVDPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQDQ 129

Query: 61  MQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYLVQGIKQNLDWVNLI 120
           M NF+ L+ +W QA VDDA +   PQ +LVA+VSNLPY++++I Y    I QNLDWVNL+
Sbjct: 130 MANFKTLIQSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAITQNLDWVNLL 189

Query: 121 SYDFYTPTSFSSTTGPSSALHNRIARSLSANFGIDAWIQSLANLPRKQIVFEIPFHGWAW 180
           SYDFYTP SF   TGPSSA HN  A +LSANFGI +W QSL NL  K+IVF IPFHGWAW
Sbjct: 190 SYDFYTPDSFRDATGPSSAFHNPKANALSANFGIRSWAQSLNNLSHKKIVFGIPFHGWAW 249

Query: 181 KLANPDKNDVFSKALGPAKGYQYISQDGQIDYSNVRKFIKENHAEYVKDDSRYSISYAYA 240
           KLAN  ++ +F+KA G A G +YI   GQI YS+V+KFI++N A     D  Y ++YAY 
Sbjct: 250 KLANSHQHALFAKADGAASG-EYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYAYY 309

Query: 241 GSTWIAYESEYIIPDKISSAKSSKVLGYFAWNIAADNEVNTLASAAQTNW 290
            +TWI +ESEY + DKI+ AK+ KVLGYFA+NIAAD+  NTLA+AA++NW
Sbjct: 310 ETTWITFESEYTVADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 358

[Close]

BLAST of MC08g_new0623 vs. ExPASy TrEMBL
Match: A0A6J1CMZ0 (oviduct-specific glycoprotein-like OS=Momordica charantia OX=3673 GN=LOC111012959 PE=4 SV=1)

HSP 1 Score: 592 bits (1526), Expect = 8.28e-214
Identity = 291/291 (100.00%), Postives = 291/291 (100.00%), Query Frame = 0


Query: 1   MSNPDVKTLLSIPGDRDVFTKMASEPTYHEAFIQSSIEVAAKGKFNDLDLQWLYPSSQEE 60
           MSNPDVKTLLSIPGDRDVFTKMASEPTYHEAFIQSSIEVAAKGKFNDLDLQWLYPSSQEE
Sbjct: 1   MSNPDVKTLLSIPGDRDVFTKMASEPTYHEAFIQSSIEVAAKGKFNDLDLQWLYPSSQEE 60

Query: 61  MQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYLVQGIKQNLDWVNLI 120
           MQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYLVQGIKQNLDWVNLI
Sbjct: 61  MQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYLVQGIKQNLDWVNLI 120

Query: 121 SYDFYTPTSFSSTTGPSSALHNRIARSLSANFGIDAWIQSLANLPRKQIVFEIPFHGWAW 180
           SYDFYTPTSFSSTTGPSSALHNRIARSLSANFGIDAWIQSLANLPRKQIVFEIPFHGWAW
Sbjct: 121 SYDFYTPTSFSSTTGPSSALHNRIARSLSANFGIDAWIQSLANLPRKQIVFEIPFHGWAW 180

Query: 181 KLANPDKNDVFSKALGPAKGYQYISQDGQIDYSNVRKFIKENHAEYVKDDSRYSISYAYA 240
           KLANPDKNDVFSKALGPAKGYQYISQDGQIDYSNVRKFIKENHAEYVKDDSRYSISYAYA
Sbjct: 181 KLANPDKNDVFSKALGPAKGYQYISQDGQIDYSNVRKFIKENHAEYVKDDSRYSISYAYA 240

Query: 241 GSTWIAYESEYIIPDKISSAKSSKVLGYFAWNIAADNEVNTLASAAQTNWS 291
           GSTWIAYESEYIIPDKISSAKSSKVLGYFAWNIAADNEVNTLASAAQTNWS
Sbjct: 241 GSTWIAYESEYIIPDKISSAKSSKVLGYFAWNIAADNEVNTLASAAQTNWS 291

[Close]

BLAST of MC08g_new0623 vs. ExPASy TrEMBL
Match: A0A6J1DYG4 (chitinase-3-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111025768 PE=4 SV=1)

HSP 1 Score: 536 bits (1380), Expect = 1.86e-190
Identity = 263/291 (90.38%), Postives = 276/291 (94.85%), Query Frame = 0


Query: 1   MSNPDVKTLLSIPGDRDVFTKMASEPTYHEAFIQSSIEVAAKGKFNDLDLQWLYPSSQEE 60
           MSNPDVKTLLSI GDRDVF KMASEPTY + FIQSSIEVAAKGKFN LDLQWLYPSS+EE
Sbjct: 70  MSNPDVKTLLSIRGDRDVFAKMASEPTYRDGFIQSSIEVAAKGKFNGLDLQWLYPSSEEE 129

Query: 61  MQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYLVQGIKQNLDWVNLI 120
           MQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVY V+ IKQNLDWVNLI
Sbjct: 130 MQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYPVEEIKQNLDWVNLI 189

Query: 121 SYDFYTPTSFSSTTGPSSALHNRIARSLSANFGIDAWIQSLANLPRKQIVFEIPFHGWAW 180
           SYDFYTP S  S TGPSSALHNR+ARSLSANFGIDAWIQSLANLPRKQIVF IPFHGWAW
Sbjct: 190 SYDFYTPASSPSATGPSSALHNRMARSLSANFGIDAWIQSLANLPRKQIVFGIPFHGWAW 249

Query: 181 KLANPDKNDVFSKALGPAKGYQYISQDGQIDYSNVRKFIKENHAEYVKDDSRYSISYAYA 240
           KLANPDK+DVFSKALGPAKG QYISQ+GQID SNV++FIK+NHA+YVKDDSRYSISYAYA
Sbjct: 250 KLANPDKHDVFSKALGPAKGDQYISQNGQIDXSNVKRFIKQNHAQYVKDDSRYSISYAYA 309

Query: 241 GSTWIAYESEYIIPDKISSAKSSKVLGYFAWNIAADNEVNTLASAAQTNWS 291
           G+TWIAYESEY IPDKISSAKSSKVLGYFAWNIAAD+EVNTLA+AAQTNWS
Sbjct: 310 GTTWIAYESEYTIPDKISSAKSSKVLGYFAWNIAADDEVNTLANAAQTNWS 360

[Close]

BLAST of MC08g_new0623 vs. ExPASy TrEMBL
Match: A0A6J1KB82 (chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493904 PE=4 SV=1)

HSP 1 Score: 350 bits (897), Expect = 4.78e-117
Identity = 174/290 (60.00%), Postives = 218/290 (75.17%), Query Frame = 0


Query: 1   MSNPDVKTLLSIPGDRDVFTKMASEPTYHEAFIQSSIEVAAKGKFNDLDLQWLYPSSQEE 60
           +SNPDVKTLLSI GD  VF  MA++PT  +AFI+SSI  A  G F+ LDLQWLYPSSQ++
Sbjct: 70  LSNPDVKTLLSIAGDAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQDQ 129

Query: 61  MQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYLVQGIKQNLDWVNLI 120
           M NF+ L+ +W QA VDDA +   PQ +LVA+VSNLPY++++I Y    I Q LDWVNL+
Sbjct: 130 MANFKTLIHSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAITQKLDWVNLL 189

Query: 121 SYDFYTPTSFSSTTGPSSALHNRIARSLSANFGIDAWIQSLANLPRKQIVFEIPFHGWAW 180
           SYDFYTP SF + TGPSSA HN  A +LSANFGI +W QSL NL  K+IVF IPFHGWAW
Sbjct: 190 SYDFYTPDSFRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLSHKKIVFGIPFHGWAW 249

Query: 181 KLANPDKNDVFSKALGPAKGYQYISQDGQIDYSNVRKFIKENHAEYVKDDSRYSISYAYA 240
           KLAN  ++ +F+KA G A G +YI   GQI YS+V+KFI++N A     D  Y ++YAY 
Sbjct: 250 KLANSHQHALFAKADGAASG-EYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYAYY 309

Query: 241 GSTWIAYESEYIIPDKISSAKSSKVLGYFAWNIAADNEVNTLASAAQTNW 290
            +TWI +ESEY + DKI+ AK+ KVLGYFA+NIAAD+  NTLA+AA++NW
Sbjct: 310 ETTWITFESEYTVADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 358

[Close]

BLAST of MC08g_new0623 vs. ExPASy TrEMBL
Match: A0A6J1KFT3 (chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493907 PE=4 SV=1)

HSP 1 Score: 348 bits (892), Expect = 2.74e-116
Identity = 174/290 (60.00%), Postives = 216/290 (74.48%), Query Frame = 0


Query: 1   MSNPDVKTLLSIPGDRDVFTKMASEPTYHEAFIQSSIEVAAKGKFNDLDLQWLYPSSQEE 60
           +SNP VKTLLSI GD  VF  MA +PT  +AFI+SSI  A  G F+ LDLQWLYPSSQ++
Sbjct: 70  LSNPGVKTLLSIAGDAAVFAAMAVDPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQDQ 129

Query: 61  MQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYLVQGIKQNLDWVNLI 120
           M NF+ L+ +W QA VDDA +   PQ +LVA+VSNLPY++++I Y    I QNLDWVNL+
Sbjct: 130 MANFKTLIQSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAITQNLDWVNLL 189

Query: 121 SYDFYTPTSFSSTTGPSSALHNRIARSLSANFGIDAWIQSLANLPRKQIVFEIPFHGWAW 180
           SYDFYTP SF   TGPSSA HN  A +LSANFGI +W QSL NL  K+IVF IPFHGWAW
Sbjct: 190 SYDFYTPDSFRDATGPSSAFHNPKANALSANFGIRSWAQSLNNLSHKKIVFGIPFHGWAW 249

Query: 181 KLANPDKNDVFSKALGPAKGYQYISQDGQIDYSNVRKFIKENHAEYVKDDSRYSISYAYA 240
           KLAN  ++ +F+KA G A G +YI   GQI YS+V+KFI++N A     D  Y ++YAY 
Sbjct: 250 KLANSHQHALFAKADGAASG-EYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYAYY 309

Query: 241 GSTWIAYESEYIIPDKISSAKSSKVLGYFAWNIAADNEVNTLASAAQTNW 290
            +TWI +ESEY + DKI+ AK+ KVLGYFA+NIAAD+  NTLA+AA++NW
Sbjct: 310 ETTWITFESEYTVADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 358

[Close]

BLAST of MC08g_new0623 vs. ExPASy TrEMBL
Match: A0A6J1G2P4 (acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE=4 SV=1)

HSP 1 Score: 347 bits (891), Expect = 3.89e-116
Identity = 174/290 (60.00%), Postives = 216/290 (74.48%), Query Frame = 0


Query: 1   MSNPDVKTLLSIPGDRDVFTKMASEPTYHEAFIQSSIEVAAKGKFNDLDLQWLYPSSQEE 60
           +SNP+VKTLLSI  D  VF  MA++PT  +AFI+SSI  A  G F+ LDLQWLYPSSQ++
Sbjct: 70  LSNPEVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQDQ 129

Query: 61  MQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYLVQGIKQNLDWVNLI 120
           M NF+ L+ +W QA VDDA +   PQ +LVA+VSNLPY++S+I Y    I Q LDWVNL+
Sbjct: 130 MDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWVNLL 189

Query: 121 SYDFYTPTSFSSTTGPSSALHNRIARSLSANFGIDAWIQSLANLPRKQIVFEIPFHGWAW 180
           SYDFYTP S  + TGPSSA HN  A +LSANFGI +W QSL NLP K+IVF IPFHGWAW
Sbjct: 190 SYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHGWAW 249

Query: 181 KLANPDKNDVFSKALGPAKGYQYISQDGQIDYSNVRKFIKENHAEYVKDDSRYSISYAYA 240
           KL N  ++ +F+KA G A G +YI   GQI YS+V+KFI++N A     D  Y ++YAY 
Sbjct: 250 KLFNSHQHTLFAKADGAATG-EYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYAYY 309

Query: 241 GSTWIAYESEYIIPDKISSAKSSKVLGYFAWNIAADNEVNTLASAAQTNW 290
            +TWI YESEY I DKI+ AK+ KVLGYFA+NIAAD+  NTLA+AA++NW
Sbjct: 310 ETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

[Close]

BLAST of MC08g_new0623 vs. TAIR 10
Match: AT4G19810.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 209.9 bits (533), Expect = 2.7e-54
Identity = 123/293 (41.98%), Postives = 168/293 (57.34%), Query Frame = 0


Query: 3   NPDVKTLLSIPG---DRDVFTKMASEPTYHEAFIQSSIEVAAKGKFNDLDLQWLYPSSQE 62
           NP VKTLLSI G   D+  +  MAS PT  ++FI SSI VA    F+ LDL W YPSS  
Sbjct: 87  NPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPSSAT 146

Query: 63  EMQNFEILLGAWRQAAVDDAKTYHRPQWILVASV--SNLPYVESSIVYLVQGIKQNLDWV 122
           EM NF  LL  WR A V +A +  +P+ +L A+V  SN  Y   S++Y V  +  +LDWV
Sbjct: 147 EMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYY---SVLYPVSAVASSLDWV 206

Query: 123 NLISYDFYTPTSFSSTTGPSSALHNRIARSLSANFGIDAWIQSLANLPRKQIVFEIPFHG 182
           NL++YDFY P  +S  TGP +AL +      S + G  +WIQ  A LP K+ V   P++G
Sbjct: 207 NLMAYDFYGP-GWSRVTGPPAALFDPSNAGPSGDAGTRSWIQ--AGLPAKKAVLGFPYYG 266

Query: 183 WAWKLANPDKNDVFSKALGPAKGYQYISQDGQIDYSNVRKFIKENHAEYVKDDSRYSISY 242
           +AW+L N + +  ++   G A     IS DG I Y  +RKFI +N A  V + +     Y
Sbjct: 267 YAWRLTNANSHSYYAPTTGAA-----ISPDGSIGYGQIRKFIVDNGATTVYNSTVVG-DY 326

Query: 243 AYAGSTWIAYESEYIIPDKISSAKSSKVLGYFAWNIAADNEVNTLASAAQTNW 291
            YAG+ WI Y+    I  K+  AK   +LGYF+W++ AD+  + L+ AA   W
Sbjct: 327 CYAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADDN-SGLSRAASQAW 366

[Close]

BLAST of MC08g_new0623 vs. TAIR 10
Match: AT4G19800.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 201.4 bits (511), Expect = 9.6e-52
Identity = 121/292 (41.44%), Postives = 166/292 (56.85%), Query Frame = 0


Query: 3   NPDVKTLLSIPG---DRDVFTKMASEPTYHEAFIQSSIEVAAKGKFNDLDLQWLYPSSQE 62
           NP VKTLLSI G   D+D F  MAS P    +FIQS+I VA    F+ LDL W YP ++E
Sbjct: 66  NPHVKTLLSIGGGNADKDAFASMASNPDSRASFIQSTITVARSYGFHGLDLDWEYPRNEE 125

Query: 63  EMQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESS---IVYLVQGIKQNLDW 122
           EM +F  LL  WR A   ++ +      IL A+V    Y  S+   + Y V  I  +LDW
Sbjct: 126 EMYDFGKLLEEWRSAVEAESNSSGTTALILTAAV----YYSSNYQGVPYPVLAISNSLDW 185

Query: 123 VNLISYDFYTPTSFSSTTGPSSALHNRIARSLSANFGIDAWIQSLANLPRKQIVFEIPFH 182
           +NL++YDFY P  +S+ TGP ++L+       S + G+  W +  A LP K+ V   P++
Sbjct: 186 INLMAYDFYGP-GWSTVTGPPASLY-LPTDGRSGDSGVRDWTE--AGLPAKKAVLGFPYY 245

Query: 183 GWAWKLANPDKNDVFSKALGPAKGYQYISQDGQIDYSNVRKFIKENHAEYVKDDSRYSIS 242
           GWAW LA+PD N   +   GPA     IS DG+I Y  ++ +I +N A  V DD      
Sbjct: 246 GWAWTLADPDVNGYDANTTGPA-----ISDDGEISYRQLQTWIVDNGATKVHDDMMVG-D 305

Query: 243 YAYAGSTWIAYESEYIIPDKISSAKSSKVLGYFAWNIAADNEVNTLASAAQT 289
           Y YAG+TWI Y+SE  I  K+  AK   +LGYF+W++  D+  + L+SA  T
Sbjct: 306 YCYAGTTWIGYDSEKSIVTKVIYAKQKGLLGYFSWHVGGDDN-SELSSAGST 342

[Close]

BLAST of MC08g_new0623 vs. TAIR 10
Match: AT4G19820.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 192.2 bits (487), Expect = 5.8e-49
Identity = 113/278 (40.65%), Postives = 156/278 (56.12%), Query Frame = 0


Query: 3   NPDVKTLLSIPGD---RDVFTKMASEPTYHEAFIQSSIEVAAKGKFNDLDLQWLYPSSQE 62
           NP VKTLLSI GD      F  MAS PT  + FI SSI++A    F+ LDL W YPS   
Sbjct: 85  NPTVKTLLSIGGDFTYNFAFASMASNPTSRKLFISSSIKLARSCGFHGLDLNWKYPSITT 144

Query: 63  EMQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYLVQGIKQNLDWVNL 122
           EM NF  LL  WR A   +A++  +P+ +L A+V    Y   S+++ V  +  +LDWVNL
Sbjct: 145 EMDNFGKLLREWRLAVEAEARSSGKPRLLLTAAVF-YSYSYYSVLHPVNAVADSLDWVNL 204

Query: 123 ISYDFYTPTSFSSTTGPSSALHNRIARSLSANFGIDAWIQSLANLPRKQIVFEIPFHGWA 182
           ++YDFY   S   T  P + L++ I    S + G+ AW Q  A LP K+ V   P +G+A
Sbjct: 205 VAYDFYESGSSRVTCSP-APLYDPITTGPSGDAGVRAWTQ--AGLPAKKAVLGFPLYGYA 264

Query: 183 WKLANPDKNDVFSKALGPAKGYQYISQDGQIDYSNVRKFIKENHAEYVKDDSRYSISYAY 242
           W L +   ++ ++ + GPA     IS DG I Y  +R+FI +N A  V  +S    +Y Y
Sbjct: 265 WCLTDAKNHNYYANSSGPA-----ISPDGSIGYDQIRRFIVDNKATMVY-NSNLVQNYCY 324

Query: 243 AGSTWIAYESEYIIPDKISSAKSSKVLGYFAWNIAADN 278
           A  TWI Y+    I  K+  AK   +LGYF+W+I AD+
Sbjct: 325 AKKTWIGYDDNQSIVMKVKYAKQRGLLGYFSWHIGADD 352

[Close]

BLAST of MC08g_new0623 vs. TAIR 10
Match: AT4G19760.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 184.5 bits (467), Expect = 1.2e-46
Identity = 114/293 (38.91%), Postives = 159/293 (54.27%), Query Frame = 0


Query: 3   NPDVKTLLSIPG---DRDVFTKMASEPTYHEAFIQSSIEVAAKGKFNDLDLQWLYPSSQE 62
           N DV+TLLSI G   D+ V   MAS     +AFI SSI++A K  F  LDL W YPS+  
Sbjct: 83  NTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGLDLAWEYPSNDV 142

Query: 63  EMQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYLVQGIKQNLDWVNL 122
           EM NF  LL  WR A V+++   ++   +L A+V   P  +  + Y V+ I  NLD+VN+
Sbjct: 143 EMTNFGKLLEEWRAAVVEESDKTNQLPLLLTAAVYYSPQYD-GVEYPVKAIADNLDFVNI 202

Query: 123 ISYDFYTPTSFSSTTGPSSALHNRIAR--SLSANFGIDAWIQSLANLPRKQIVFEIPFHG 182
           ++YDFY P  +S  TGP +AL +  +     S N G+  W+   A LP K+ V   P+ G
Sbjct: 203 MAYDFYGP-GWSPVTGPPAALFHDPSNPAGRSGNSGLRKWLDE-AKLPPKKAVLGFPYCG 262

Query: 183 WAWKLANPDKNDVFSKALGPAKGYQYISQDGQIDYSNVRKFIKENHAEYVKDDSRYSISY 242
           WAW L + + N   +   G A     IS DG I Y+ +R +I +N A    D +     Y
Sbjct: 263 WAWTLEDAENNGYDAATDGAA-----ISPDGSITYAKIRNYIVDNGAATFHDPAVIGF-Y 322

Query: 243 AYAGSTWIAYESEYIIPDKISSAKSSKVLGYFAWNIAADNEVNTLASAAQTNW 291
            Y G+TWI Y+    I  K+  AK + +LGYF+W++ AD     L+ AA   W
Sbjct: 323 CYVGNTWIGYDDNQSIVYKVKYAKFTGLLGYFSWHVGADYNCG-LSRAASIAW 365

[Close]

BLAST of MC08g_new0623 vs. TAIR 10
Match: AT4G19750.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 177.2 bits (448), Expect = 1.9e-44
Identity = 110/280 (39.29%), Postives = 152/280 (54.29%), Query Frame = 0


Query: 3   NPDVKTLLSIPG---DRDVFTKMASEPTYHEAFIQSSIEVAAKGKFNDLDLQWLYPSSQE 62
           N DV+TLLSI G   D+ V   MAS     +AFI SSI++A K  F  LDL W YPS+  
Sbjct: 75  NTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGLDLAWEYPSNDV 134

Query: 63  EMQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLP--YVESSIVYLVQGIKQNLDWV 122
           EM NF  L+  WR A V+++   ++   +L A+V   P  Y E    Y VQ I  NLD+V
Sbjct: 135 EMANFGKLVKEWRAAVVEESDRTNQLPLLLTAAVYYSPDYYGEE---YPVQAIADNLDFV 194

Query: 123 NLISYDFYTPTSFSSTTGPSSALHN-RIARSLSANFGIDAWIQSLANLPRKQIVFEIPFH 182
           N+++YDFY P  +S  TGP +AL +       S + G+  W++  A LP K+ V    + 
Sbjct: 195 NIMAYDFYGP-GWSPVTGPPAALFDPSNPAGRSGDSGLSKWLE--AKLPAKKAVLGFSYC 254

Query: 183 GWAWKLANPDKNDVFSKALGPAKGYQYISQDGQIDYSNVRKFIKENHAEYVKDDSRYSIS 242
           GWAW L + + N   +   G A     IS DG I Y+ +R +I +N A    D +     
Sbjct: 255 GWAWTLEDAENNGYDAATDGAA-----ISSDGSITYAKIRNYIIDNGAATFHDPAVIGF- 314

Query: 243 YAYAGSTWIAYESEYIIPDKISSAKSSKVLGYFAWNIAAD 277
           Y Y G+TWI Y+    I  K+  AK   +LGYF+W++ AD
Sbjct: 315 YCYVGTTWIGYDDNQSIVSKVRYAKLKGLLGYFSWHVGAD 342

The following BLAST results are available for this feature:

BLAST of MC08g_new0623 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81862	3.8e-53	41.98	Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1	[more]
A0A1B1J8Z2	3.1e-39	33.89	Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2	[more]
A0A072VEP0	2.9e-37	32.67	Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1	[more]
U5N4E3	2.3e-34	32.76	Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2	[more]
A0A072UR65	9.8e-33	30.95	Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1	[more]

BLAST of MC08g_new0623 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142964.1	1.71e-213	100.00	oviduct-specific glycoprotein-like [Momordica charantia]	[more]
XP_022159350.1	3.84e-190	90.38	chitinase-3-like protein 2 [Momordica charantia]	[more]
XP_022999577.1	9.88e-117	60.00	chitotriosidase-1-like [Cucurbita maxima]	[more]
KAG7030350.1	5.66e-116	59.66	Chitotriosidase-1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022999580.1	5.66e-116	60.00	chitotriosidase-1-like [Cucurbita maxima]	[more]

BLAST of MC08g_new0623 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CMZ0	8.28e-214	100.00	oviduct-specific glycoprotein-like OS=Momordica charantia OX=3673 GN=LOC11101295...	[more]
A0A6J1DYG4	1.86e-190	90.38	chitinase-3-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111025768 PE=4 S...	[more]
A0A6J1KB82	4.78e-117	60.00	chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493904 PE=4 SV=1	[more]
A0A6J1KFT3	2.74e-116	60.00	chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493907 PE=4 SV=1	[more]
A0A6J1G2P4	3.89e-116	60.00	acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE...	[more]

BLAST of MC08g_new0623 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G19810.1	2.7e-54	41.98	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19800.1	9.6e-52	41.44	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19820.1	5.8e-49	40.65	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19760.1	1.2e-46	38.91	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19750.1	1.9e-44	39.29	Glycosyl hydrolase family protein with chitinase insertion domain	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011583	Chitinase II	SMART	SM00636	2g34	coord: 1..276 e-value: 7.3E-25 score: 98.6
IPR029070	Chitinase insertion domain superfamily	GENE3D	3.10.50.10		coord: 175..247 e-value: 5.5E-61 score: 208.8
IPR029070	Chitinase insertion domain superfamily	SUPERFAMILY	54556	Chitinase insertion domain	coord: 177..247
IPR001223	Glycoside hydrolase family 18, catalytic domain	PFAM	PF00704	Glyco_hydro_18	coord: 2..276 e-value: 9.8E-47 score: 160.2
IPR001223	Glycoside hydrolase family 18, catalytic domain	PROSITE	PS51910	GH18_2	coord: 1..291 score: 35.302048
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 2..277 e-value: 5.5E-61 score: 208.8
None	No IPR available	PANTHER	PTHR11177	CHITINASE	coord: 2..286
None	No IPR available	PANTHER	PTHR11177:SF351	CHITINASE-LIKE PROTEIN-RELATED	coord: 2..286
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 3..278

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g_new0623.1	MC08g_new0623.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0008061	chitin binding
molecular_function	GO:0016787	hydrolase activity