MC08g_new0607 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g_new0607 vs. NCBI nr
Match: KAG6599284.1 (putative nucleolar protein 5-2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 167 bits (423), Expect = 9.32e-43
Identity = 118/299 (39.46%), Postives = 143/299 (47.83%), Query Frame = 0

Query: 1   MADFRYGFFIRPENKKLGLHPDEKSKTSHVSDHKCKPAIVDAEGKKQPITVLSDETYVIK 60
           MADFRYGFF RP+NK L    +EK+ TSH+ D K KPA VDA G++ P  VL    YV  
Sbjct: 1   MADFRYGFFCRPDNKNLEPQQEEKNTTSHIPDPKYKPAHVDAGGRRLPTVVLPRPRYVTT 60

Query: 61  KDTYVEEFYSSLGEESQYSYTMEYEPVTYYGRRHDKARNQHPSL-ARDRPTEVDKFITDV 120
            +TY EEFYS+  +  QYS+T   EPV  YG  HDK   +HPS  A +RPTE ++ +  V
Sbjct: 61  TETYTEEFYSNSRDGFQYSHTKNSEPVKSYGLHHDKGI-RHPSRPALNRPTEFNRLLVKV 120

Query: 121 QMEASKPMSPTYRRQISNTRVEDDIINGCHDDYNKKIANKPSGYAKQHAKHRNGYNSNND 180
           +ME                  ED +                        KH         
Sbjct: 121 EME------------------EDQV------------------------KHE-------- 180

Query: 181 EYDDSDDEPRTMTSHTFGLAPLGWARPSDELPARGLSLSGPMNDIGKAIECLKEAANLQS 240
                                              + +S PMNDI KAIE  KEA NL  
Sbjct: 181 -----------------------------------MRISKPMNDIEKAIEFFKEAVNLPY 213

Query: 241 GKS----ALDHRKKDGYTKTIDSNEAARQYGKLIPLPPVQGAHVATIDSKEAARKYKGT 294
            K+    A DH KKDGYTKTIDSNEAAR++G     PPV  A+ ATI+ KEAARKYK +
Sbjct: 241 CKNNACHAPDHPKKDGYTKTIDSNEAARRFGNFGVPPPVPSANGATINCKEAARKYKAS 213

BLAST of MC08g_new0607 vs. NCBI nr
Match: KGN60838.1 (hypothetical protein Csa_019431 [Cucumis sativus])

HSP 1 Score: 140 bits (352), Expect = 1.30e-36
Identity = 108/301 (35.88%), Postives = 132/301 (43.85%), Query Frame = 0

Query: 1   MADFRYGFFIRPENKKLGLHPDE-KSKTSHVSDHKCKPAIVDAEGKKQPITVLSDETYVI 60
           MADFRYGFF+RP+NKK+  +P E KSK  H+SD K KP  +DA G++QPI +L    YV 
Sbjct: 1   MADFRYGFFVRPDNKKIESNPAELKSKAGHISDPKSKPPYLDAGGRQQPIAILPRTRYVT 60

Query: 61  KKDTYVEEFYSSLGEESQYSYTMEYEPVTYYGRRHDKAR-NQHPSLARDRPTEVDKFITD 120
             + Y EE +           T +YEPV    +  DK   N H  LA ++  E +K + +
Sbjct: 61  PIEKYTEEVH-----------TKKYEPV----KNSDKGTLNSH--LAINQRIEFNKLLAN 120

Query: 121 VQMEASKPMSPTYRRQISNTRVEDDIINGCHDDYNKKIANKPSGYAKQHAKHRNGYNSNN 180
           VQ EA  P                         Y  ++                      
Sbjct: 121 VQKEARLPK------------------------YEMRL---------------------- 180

Query: 181 DEYDDSDDEPRTMTSHTFGLAPLGWARPSDELPARGLSLSGPMNDIGKAIECLKEAANLQ 240
                                                 LS PM DIGKAIECLKE  NL 
Sbjct: 181 --------------------------------------LSEPMTDIGKAIECLKEVVNLD 200

Query: 241 SGKS---ALDHRKKDGYTKTIDSNEAARQYGKLIPLPPVQGAHVATIDSKEAARKYKGTK 296
             K+   A   R+KD  TKTIDS EAAR+YGK     PV  A+VATID KEAARKY G  
Sbjct: 241 CSKNNACAASARRKDSCTKTIDSKEAARRYGKFGAPVPVSNANVATIDCKEAARKYNGAA 200

BLAST of MC08g_new0607 vs. NCBI nr
Match: KAG6751695.1 (hypothetical protein POTOM_043894 [Populus tomentosa])

HSP 1 Score: 128 bits (321), Expect = 6.02e-31
Identity = 109/318 (34.28%), Postives = 153/318 (48.11%), Query Frame = 0

Query: 1   MADFRYGF-----FIRPENKKLGLHPDEKSKTSHVSDHKCKPAIVDAEGKKQP-ITVLSD 60
           MA F Y +     +  P +   G   D  SKT +V DH C+P I+DA G+K+P I+  +D
Sbjct: 1   MAGFGYSYRGYSSYDPPTSDDYGCSDDYGSKTGYVPDHVCRPVIIDANGRKRPVISYRAD 60

Query: 61  ET---YVIKKDTYVEEFYSSLGEESQYSYTMEYEPVTYYGRRHDKARNQHPSLARD-RPT 120
           +    YV K +TY ++      E        E+E V  +   +  A+N+    + D RP 
Sbjct: 61  QNADHYVTKTETYYQQHVHPPVE-------YEHEMVPRWSEENPVAKNKFRLASVDGRPP 120

Query: 121 EVDKFITDVQMEASKPMS-----PTYRRQISNTRVEDDIINGC--HDDYNKKIANKPSGY 180
           +V++FIT VQ+EAS+P        +YRR        D   +G   H  ++ K   KP   
Sbjct: 121 KVEEFITKVQIEASRPKERAPWDASYRRPTPKPTGYDGYTDGYDEHSGFSNKDLLKPGVN 180

Query: 181 AKQHAKHRNGYNSNNDEYDDSDDEPRTMTSHTFGLAPLGWARPSDELPAR--GLSLSGPM 240
           A     HRN    N D+Y          T +T G    GWARPS    A     +LSG  
Sbjct: 181 A-----HRN---DNYDDYYRKQGSNMEPTMNTSG----GWARPSHSTWAAPPNATLSGAT 240

Query: 241 NDIGKAIECLKEAANLQSGKSALDHRKKDGYTKTIDSNEAARQYGKL--IPLPPVQG-AH 296
           NDI  A+E L++AA      S     ++  + KTIDS EAAR+YG       P  +  ++
Sbjct: 241 NDISAAVELLRQAAKPSFSTSPPSRYREPTHAKTIDSKEAARRYGNFNYSSRPYARDDSY 299

BLAST of MC08g_new0607 vs. NCBI nr
Match: KAA0063635.1 (hypothetical protein E6C27_scaffold329G001480 [Cucumis melo var. makuwa] >TYK18391.1 hypothetical protein E5676_scaffold456G001150 [Cucumis melo var. makuwa])

HSP 1 Score: 125 bits (313), Expect = 7.67e-31
Identity = 95/299 (31.77%), Postives = 128/299 (42.81%), Query Frame = 0

Query: 1   MADFRYGFFIRPENKKLGLHPDEKSKTSHVSDHKCKPAIVDAEGKKQPITVLSDETYVIK 60
           MADFRYGFF+RP+NKK+  +P+EKS+  H+S+ K KP  +DA G+++P  +L    YV  
Sbjct: 1   MADFRYGFFVRPDNKKIESNPEEKSRAGHISNPKSKPPNLDAGGRQRPTAILPRTRYVTP 60

Query: 61  KDTYVEEFYSSLGEESQYSYTMEYEPVTYYGRRHDKARNQHPSLARDRPTEVDKFITDVQ 120
            + + EE +           T +YEP+    +  DK      +LA ++  E +K +   Q
Sbjct: 61  IEKHTEEVH-----------TKKYEPM----KNSDKG-TLDSNLAINQRIEFNKLLAKAQ 120

Query: 121 MEASKPMSPTYRRQISNTRVEDDIINGCHDDYNKKIANKPSGYAKQHAKHRNGYNSNNDE 180
            E   P                         Y  ++                        
Sbjct: 121 EEVRLPK------------------------YEMRL------------------------ 180

Query: 181 YDDSDDEPRTMTSHTFGLAPLGWARPSDELPARGLSLSGPMNDIGKAIECLKEAANLQSG 240
                                               LS PMNDIGKAIECLKEA NL   
Sbjct: 181 ------------------------------------LSEPMNDIGKAIECLKEAVNLDCS 199

Query: 241 KS---ALDHRKKDGYTKTIDSNEAARQYGKLIPLPPVQGAHVATIDSKEAARKYKGTKV 296
           K+   +  +  KD  TKTIDS EAAR+YG    L  V  A+ ATI+ KEAARKY G +V
Sbjct: 241 KNNACSASYHHKDSCTKTIDSKEAARRYGTFGALGRVSSANEATINCKEAARKYNGAEV 199

BLAST of MC08g_new0607 vs. NCBI nr
Match: XP_011003819.1 (PREDICTED: uncharacterized protein LOC105110465 [Populus euphratica])

HSP 1 Score: 126 bits (317), Expect = 1.69e-30
Identity = 109/294 (37.07%), Postives = 147/294 (50.00%), Query Frame = 0

Query: 22  DEKSKTSHVSDHKCKPAIVDAEGKKQPI-TVLSDET---YVIKKDTYVEEFYSSLGEESQ 81
           D   +T +V +H C+P I+DA+G+K+PI +  +D+    YV K +TY +E          
Sbjct: 16  DYGRRTVYVPEHVCRPVIIDADGRKRPIISYRADQNADHYVTKTETYYQELVYP------ 75

Query: 82  YSYTMEYEP--VTYYGRRHDKARNQHP-SLARDRPTEVDKFITDVQMEASKPMS-----P 141
               MEYE   V  +   +  A N+   + A DRP +V++FIT VQ+EAS+P        
Sbjct: 76  ---PMEYEDKHVPRWAEENRVAENKFRLASADDRPQKVEEFITKVQIEASRPKEYAPWDA 135

Query: 142 TYRRQISNTRVED--DIINGCHDDYNKKIANKPSGYAKQHAKHRNGYNSNNDEYDDSDDE 201
           +YRRQ   +   D  D  NG    +N K   KP G A     HRN  ++N D+Y      
Sbjct: 136 SYRRQTPKSTGYDGYDEYNG----FNNKDLLKPRGDA-----HRN--DNNYDDYYRKQGS 195

Query: 202 PRTMTSHTFGLAPLGWARPSDELPAR--GLSLSGPMNDIGKAIECLKEAANLQSGKSALD 261
               T  T G    GWARPS    A      LSG  NDI  A+E L+EAA      S   
Sbjct: 196 NMEPTMKTSG----GWARPSHSTWAAPPNAPLSGATNDISAAVELLREAAKPSVTTSPPS 255

Query: 262 HRKKDGYTKTIDSNEAARQYGKL-IPLPPV--QGAHVATIDSKEAARKYKGTKV 296
             ++  Y  TIDS EAAR+YGK      P     ++ +TIDS+EAARKY G+ V
Sbjct: 256 RYREPAYANTIDSKEAARRYGKFNFSSRPYARDDSYTSTIDSREAARKYSGSAV 285

BLAST of MC08g_new0607 vs. ExPASy TrEMBL
Match: A0A0A0LG87 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G012700 PE=4 SV=1)

HSP 1 Score: 140 bits (352), Expect = 6.27e-37
Identity = 108/301 (35.88%), Postives = 132/301 (43.85%), Query Frame = 0

Query: 1   MADFRYGFFIRPENKKLGLHPDE-KSKTSHVSDHKCKPAIVDAEGKKQPITVLSDETYVI 60
           MADFRYGFF+RP+NKK+  +P E KSK  H+SD K KP  +DA G++QPI +L    YV 
Sbjct: 1   MADFRYGFFVRPDNKKIESNPAELKSKAGHISDPKSKPPYLDAGGRQQPIAILPRTRYVT 60

Query: 61  KKDTYVEEFYSSLGEESQYSYTMEYEPVTYYGRRHDKAR-NQHPSLARDRPTEVDKFITD 120
             + Y EE +           T +YEPV    +  DK   N H  LA ++  E +K + +
Sbjct: 61  PIEKYTEEVH-----------TKKYEPV----KNSDKGTLNSH--LAINQRIEFNKLLAN 120

Query: 121 VQMEASKPMSPTYRRQISNTRVEDDIINGCHDDYNKKIANKPSGYAKQHAKHRNGYNSNN 180
           VQ EA  P                         Y  ++                      
Sbjct: 121 VQKEARLPK------------------------YEMRL---------------------- 180

Query: 181 DEYDDSDDEPRTMTSHTFGLAPLGWARPSDELPARGLSLSGPMNDIGKAIECLKEAANLQ 240
                                                 LS PM DIGKAIECLKE  NL 
Sbjct: 181 --------------------------------------LSEPMTDIGKAIECLKEVVNLD 200

Query: 241 SGKS---ALDHRKKDGYTKTIDSNEAARQYGKLIPLPPVQGAHVATIDSKEAARKYKGTK 296
             K+   A   R+KD  TKTIDS EAAR+YGK     PV  A+VATID KEAARKY G  
Sbjct: 241 CSKNNACAASARRKDSCTKTIDSKEAARRYGKFGAPVPVSNANVATIDCKEAARKYNGAA 200

BLAST of MC08g_new0607 vs. ExPASy TrEMBL
Match: A0A5A7VCJ6 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G001150 PE=4 SV=1)

HSP 1 Score: 125 bits (313), Expect = 3.71e-31
Identity = 95/299 (31.77%), Postives = 128/299 (42.81%), Query Frame = 0

Query: 1   MADFRYGFFIRPENKKLGLHPDEKSKTSHVSDHKCKPAIVDAEGKKQPITVLSDETYVIK 60
           MADFRYGFF+RP+NKK+  +P+EKS+  H+S+ K KP  +DA G+++P  +L    YV  
Sbjct: 1   MADFRYGFFVRPDNKKIESNPEEKSRAGHISNPKSKPPNLDAGGRQRPTAILPRTRYVTP 60

Query: 61  KDTYVEEFYSSLGEESQYSYTMEYEPVTYYGRRHDKARNQHPSLARDRPTEVDKFITDVQ 120
            + + EE +           T +YEP+    +  DK      +LA ++  E +K +   Q
Sbjct: 61  IEKHTEEVH-----------TKKYEPM----KNSDKG-TLDSNLAINQRIEFNKLLAKAQ 120

Query: 121 MEASKPMSPTYRRQISNTRVEDDIINGCHDDYNKKIANKPSGYAKQHAKHRNGYNSNNDE 180
            E   P                         Y  ++                        
Sbjct: 121 EEVRLPK------------------------YEMRL------------------------ 180

Query: 181 YDDSDDEPRTMTSHTFGLAPLGWARPSDELPARGLSLSGPMNDIGKAIECLKEAANLQSG 240
                                               LS PMNDIGKAIECLKEA NL   
Sbjct: 181 ------------------------------------LSEPMNDIGKAIECLKEAVNLDCS 199

Query: 241 KS---ALDHRKKDGYTKTIDSNEAARQYGKLIPLPPVQGAHVATIDSKEAARKYKGTKV 296
           K+   +  +  KD  TKTIDS EAAR+YG    L  V  A+ ATI+ KEAARKY G +V
Sbjct: 241 KNNACSASYHHKDSCTKTIDSKEAARRYGTFGALGRVSSANEATINCKEAARKYNGAEV 199

BLAST of MC08g_new0607 vs. ExPASy TrEMBL
Match: E5GBR2 (Uncharacterized protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 123 bits (309), Expect = 1.45e-30
Identity = 94/299 (31.44%), Postives = 127/299 (42.47%), Query Frame = 0

Query: 1   MADFRYGFFIRPENKKLGLHPDEKSKTSHVSDHKCKPAIVDAEGKKQPITVLSDETYVIK 60
           MADFRYGFF+RP+NKK+  +P+EKS+  H+S+ K KP  +D  G+++P  +L    YV  
Sbjct: 1   MADFRYGFFVRPDNKKIESNPEEKSRAGHISNPKSKPPNLDVGGRQRPTAILPRTRYVTP 60

Query: 61  KDTYVEEFYSSLGEESQYSYTMEYEPVTYYGRRHDKARNQHPSLARDRPTEVDKFITDVQ 120
            + + EE +           T +YEP+    +  DK      +LA ++  E +K +   Q
Sbjct: 61  IEKHTEEVH-----------TKKYEPM----KNSDKG-TLDSNLAINQRIEFNKLLAKAQ 120

Query: 121 MEASKPMSPTYRRQISNTRVEDDIINGCHDDYNKKIANKPSGYAKQHAKHRNGYNSNNDE 180
            E   P                         Y  ++                        
Sbjct: 121 EEVRLPK------------------------YEMRL------------------------ 180

Query: 181 YDDSDDEPRTMTSHTFGLAPLGWARPSDELPARGLSLSGPMNDIGKAIECLKEAANLQSG 240
                                               LS PMNDIGKAIECLKEA NL   
Sbjct: 181 ------------------------------------LSEPMNDIGKAIECLKEAVNLDCS 199

Query: 241 KS---ALDHRKKDGYTKTIDSNEAARQYGKLIPLPPVQGAHVATIDSKEAARKYKGTKV 296
           K+   +  +  KD  TKTIDS EAAR+YG    L  V  A+ ATI+ KEAARKY G +V
Sbjct: 241 KNNACSASYHHKDSCTKTIDSKEAARRYGTFGALGRVSSANEATINCKEAARKYNGAEV 199

BLAST of MC08g_new0607 vs. ExPASy TrEMBL
Match: A0A4U5R1K5 (Uncharacterized protein OS=Populus alba OX=43335 GN=D5086_0000012360 PE=4 SV=1)

HSP 1 Score: 125 bits (315), Expect = 2.19e-30
Identity = 108/318 (33.96%), Postives = 152/318 (47.80%), Query Frame = 0

Query: 1   MADFRYGF-----FIRPENKKLGLHPDEKSKTSHVSDHKCKPAIVDAEGKKQP-ITVLSD 60
           MA F Y +     +  P +   G   D  SKT +V DH C+P I+DA G+K+P I+  +D
Sbjct: 1   MAGFGYSYRGYSSYDPPTSDDYGCSDDYGSKTGYVPDHVCRPVIIDANGRKRPVISYRAD 60

Query: 61  ET---YVIKKDTYVEEFYSSLGEESQYSYTMEYEPVTYYGRRHDKARNQHPSLARD-RPT 120
           +    YV K +TY ++      E        E++ V  +   +  A N+    + D RP 
Sbjct: 61  QNADHYVTKTETYYQQHVHPPVE-------YEHKMVPRWSEENPVAENKFRLASVDGRPP 120

Query: 121 EVDKFITDVQMEASKPMS-----PTYRRQISNTRVEDDIINGC--HDDYNKKIANKPSGY 180
           +V++FIT VQ+EAS+P        +YRR        D   +G   H  ++ K   KP   
Sbjct: 121 KVEEFITKVQIEASRPKERAPWDASYRRPTPKPTGYDGYTDGYDEHSGFSNKDLLKPGVN 180

Query: 181 AKQHAKHRNGYNSNNDEYDDSDDEPRTMTSHTFGLAPLGWARPSDELPAR--GLSLSGPM 240
           A     HRN    N D+Y          T +T G    GWARPS    A     +LSG  
Sbjct: 181 A-----HRN---DNYDDYYRKQGSNMEPTMNTSG----GWARPSHSTWAAPPNATLSGAT 240

Query: 241 NDIGKAIECLKEAANLQSGKSALDHRKKDGYTKTIDSNEAARQYGKL--IPLPPVQG-AH 296
           NDI  A+E L++AA      S     ++  + KTIDS EAAR+YG       P  +  ++
Sbjct: 241 NDISAAVELLRQAAKPSFSTSPPSRYREPTHAKTIDSKEAARRYGNFNYSSRPYARDDSY 299

BLAST of MC08g_new0607 vs. ExPASy TrEMBL
Match: A9PCB5 (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_013G032900 PE=2 SV=1)

HSP 1 Score: 118 bits (296), Expect = 1.16e-27
Identity = 101/294 (34.35%), Postives = 141/294 (47.96%), Query Frame = 0

Query: 22  DEKSKTSHVSDHKCKPAIVDAEGKKQPITVL----SDETYVIKKDTYVEEFYSSLGEESQ 81
           D  SKT +V DH C+P I+DA+G+K+PI       + + YV K +TY ++      E   
Sbjct: 21  DYGSKTGYVPDHVCRPVIIDADGRKRPIISYRVDQNADQYVTKTETYYQQHVHPPKE--- 80

Query: 82  YSYTMEYEPVTYYGRRHDKARNQ--HPSLARD-RPTEVDKFITDVQMEASKPMS-----P 141
                E + V  +   +  A N+   P  + D RP +V++FIT VQ+EAS+P        
Sbjct: 81  ----YEDKEVPRWSEEYHGAENKFRRPLASADGRPQKVEEFITKVQVEASRPQEYAPWDA 140

Query: 142 TYRRQISNTRVEDDIINGCHD--DYNKKIANKPSGYAKQHAKHRNGYNSNNDEYDDSDDE 201
           +YRRQ   +   D    G  +   ++ K   KP G A     HRN    N D+Y      
Sbjct: 141 SYRRQTPKSTGYDGYTGGYDERSGFSNKDLLKPGGNA-----HRN---DNYDDYYRKQGS 200

Query: 202 PRTMTSHTFGLAPLGWARPSDELPAR--GLSLSGPMNDIGKAIECLKEAANLQSGKSALD 261
               T +T G    GWARPS    A      LSG  NDI  A+  L+E A      S   
Sbjct: 201 NMEPTMNTSG----GWARPSHSTWAAPPNAPLSGATNDISAAVGLLREVAKPSFSTSPPS 260

Query: 262 HRKKDGYTKTIDSNEAARQYGKL--IPLPPVQG-AHVATIDSKEAARKYKGTKV 296
             ++  +  TIDS EAAR+YG       P  +  ++ +TIDS+EAARKY G+ V
Sbjct: 261 RYREPTHANTIDSKEAARRYGNFNYSSRPYTRDDSYTSTIDSREAARKYSGSAV 295