MC08g_new0561 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g_new0561
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description(R)-mandelonitrile lyase 1-like
LocationMC08: 29995629 .. 29996273 (+)
RNA-Seq ExpressionMC08g_new0561
SyntenyMC08g_new0561
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGTTTGTGTACGATGCCAATAATTTGCCAGAAAAGGAGGAATACGACTACATAATAATAGGGGGAGGAACGGCAAGCTGTCCATTAGCTGTAACAATATCATCAAACTTCTCTGTCCTCCTTCTTGAAAGAGGTTCTGTCCCTTATAACTTCTCTACTGTACTTAACATACAAGGTCTTTCAAATGTTTTCACCAACGACGATGACGGCGAAAATCCCTTCCAGCGGTTCATATCAGAGGACGGTGTGGAGAACATAAGAGGGTGGATCCTCGGCGGCGGCAGCATGATCAATGCTGGCTTCTACTCGAGGGCTCATAAAGAGTTCTTTGAAATGCAAGGCATGGAATGGGACATGGAAATGGTGGAGCAGGCGTATAAATGTGTGGAAGAGACTGTGGTGTCTCGCCCGAGCTTGAGTTCTTGGCAATCTGCTTTTAGAAGTGCTTTGTTGGAAAGTGGAGTTGGCCCTGATAATGATTTTGATTTGAGAAACCTTGTGGGGACTAAAATTGGAGGTTCCATATTTGGTAGGGAAGGGAATAGACATGGAGCCGTGGAGCTTCTCAATAAAGCTAATCCCGTAAATCTTAAAGTTGTCGTCCGTGCCAAAGTTGAAAGAATCATTTTCTTTGGTAAGTGA

mRNA sequence

ATGAAGTTTGTGTACGATGCCAATAATTTGCCAGAAAAGGAGGAATACGACTACATAATAATAGGGGGAGGAACGGCAAGCTGTCCATTAGCTGTAACAATATCATCAAACTTCTCTGTCCTCCTTCTTGAAAGAGGTTCTGTCCCTTATAACTTCTCTACTGTACTTAACATACAAGGTCTTTCAAATGTTTTCACCAACGACGATGACGGCGAAAATCCCTTCCAGCGGTTCATATCAGAGGACGGTGTGGAGAACATAAGAGGGTGGATCCTCGGCGGCGGCAGCATGATCAATGCTGGCTTCTACTCGAGGGCTCATAAAGAGTTCTTTGAAATGCAAGGCATGGAATGGGACATGGAAATGGTGGAGCAGGCGTATAAATGTGTGGAAGAGACTGTGGTGTCTCGCCCGAGCTTGAGTTCTTGGCAATCTGCTTTTAGAAGTGCTTTGTTGGAAAGTGGAGTTGGCCCTGATAATGATTTTGATTTGAGAAACCTTGTGGGGACTAAAATTGGAGGTTCCATATTTGGTAGGGAAGGGAATAGACATGGAGCCGTGGAGCTTCTCAATAAAGCTAATCCCGTAAATCTTAAAGTTGTCGTCCGTGCCAAAGTTGAAAGAATCATTTTCTTTGGTAAGTGA

Coding sequence (CDS)

ATGAAGTTTGTGTACGATGCCAATAATTTGCCAGAAAAGGAGGAATACGACTACATAATAATAGGGGGAGGAACGGCAAGCTGTCCATTAGCTGTAACAATATCATCAAACTTCTCTGTCCTCCTTCTTGAAAGAGGTTCTGTCCCTTATAACTTCTCTACTGTACTTAACATACAAGGTCTTTCAAATGTTTTCACCAACGACGATGACGGCGAAAATCCCTTCCAGCGGTTCATATCAGAGGACGGTGTGGAGAACATAAGAGGGTGGATCCTCGGCGGCGGCAGCATGATCAATGCTGGCTTCTACTCGAGGGCTCATAAAGAGTTCTTTGAAATGCAAGGCATGGAATGGGACATGGAAATGGTGGAGCAGGCGTATAAATGTGTGGAAGAGACTGTGGTGTCTCGCCCGAGCTTGAGTTCTTGGCAATCTGCTTTTAGAAGTGCTTTGTTGGAAAGTGGAGTTGGCCCTGATAATGATTTTGATTTGAGAAACCTTGTGGGGACTAAAATTGGAGGTTCCATATTTGGTAGGGAAGGGAATAGACATGGAGCCGTGGAGCTTCTCAATAAAGCTAATCCCGTAAATCTTAAAGTTGTCGTCCGTGCCAAAGTTGAAAGAATCATTTTCTTTGGTAAGTGA

Protein sequence

MKFVYDANNLPEKEEYDYIIIGGGTASCPLAVTISSNFSVLLLERGSVPYNFSTVLNIQGLSNVFTNDDDGENPFQRFISEDGVENIRGWILGGGSMINAGFYSRAHKEFFEMQGMEWDMEMVEQAYKCVEETVVSRPSLSSWQSAFRSALLESGVGPDNDFDLRNLVGTKIGGSIFGREGNRHGAVELLNKANPVNLKVVVRAKVERIIFFGK
Homology
BLAST of MC08g_new0561 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 4.5e-59
Identity = 107/211 (50.71%), Postives = 148/211 (70.14%), Query Frame = 0

Query: 1   MKFVYDANNLPEKEEYDYIIIGGGTASCPLAVTISSNFSVLLLERGSVPYNFSTVLNIQG 60
           + FVYDA +   +  YDYII+GGGTA CPLA T+S+N+SVL+LERGS+P  +  +L   G
Sbjct: 39  LSFVYDATDPELEGSYDYIIVGGGTAGCPLAATLSANYSVLVLERGSLPTEYPNLLISDG 98

Query: 61  LSNVFTNDDDGENPFQRFISEDGVENIRGWILGGGSMINAGFYSRAHKEFFEMQGMEWDM 120
                  +DDG+ P +RF+SEDG++N+RG +LGG SMINAG Y RA+  FF   G+EWDM
Sbjct: 99  FVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSMINAGVYVRANTSFFNQTGIEWDM 158

Query: 121 EMVEQAYKCVEETVVSRPSLSSWQSAFRSALLESGVGPDNDFDLRNLVGTKIGGSIFGRE 180
           ++V Q Y+ VE+T+V  P   +WQ+   +A LE+G+ P+N F + +L GT++ GS F   
Sbjct: 159 DLVNQTYEWVEDTIVFEPDSQTWQTVIGTAYLEAGILPNNGFSVDHLAGTRLTGSTFDNN 218

Query: 181 GNRHGAVELLNKANPVNLKVVVRAKVERIIF 212
           G RH + ELLNK +P NL+V V+A VE+IIF
Sbjct: 219 GTRHASDELLNKGDPNNLRVAVQAAVEKIIF 249

BLAST of MC08g_new0561 vs. ExPASy Swiss-Prot
Match: O50048 ((R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 9.9e-59
Identity = 107/211 (50.71%), Postives = 146/211 (69.19%), Query Frame = 0

Query: 1   MKFVYDANNLPEKEEYDYIIIGGGTASCPLAVTISSNFSVLLLERGSVPYNFSTVLNIQG 60
           ++FVYDAN+   +  YDYII+GGGTA CPLA T+S+N+SVL+LERG++P  +  +L   G
Sbjct: 40  LEFVYDANDTELEGTYDYIIVGGGTAGCPLAATLSANYSVLVLERGTLPTEYPNLLTSDG 99

Query: 61  LSNVFTNDDDGENPFQRFISEDGVENIRGWILGGGSMINAGFYSRAHKEFFEMQGMEWDM 120
                  +DDG+ P +RF+S DG++N+RG +LGG SMINAG Y RA+  FF   G+EWDM
Sbjct: 100 FIYNLQQEDDGQTPVERFVSGDGIDNVRGRVLGGTSMINAGVYVRANTSFFNQTGIEWDM 159

Query: 121 EMVEQAYKCVEETVVSRPSLSSWQSAFRSALLESGVGPDNDFDLRNLVGTKIGGSIFGRE 180
           ++V + Y  VE+T+V +P    WQ+   +A LE G+ PDN F L +L GT++ GS F   
Sbjct: 160 DLVNKTYDWVEDTIVFKPDFQFWQNLTGTAFLEVGILPDNGFSLDHLEGTRLTGSTFDNN 219

Query: 181 GNRHGAVELLNKANPVNLKVVVRAKVERIIF 212
           G RH + ELLNK +P NL+V V A VE+IIF
Sbjct: 220 GTRHASDELLNKGDPNNLRVAVHAAVEKIIF 250

BLAST of MC08g_new0561 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 2.9e-58
Identity = 111/210 (52.86%), Postives = 144/210 (68.57%), Query Frame = 0

Query: 1   MKFVYDANNLPEKEEYDYIIIGGGTASCPLAVTISSNFSVLLLERGSVPYNFSTVLNIQG 60
           M+F+ +A +   ++ YDYII+GGGTA CPLA T+S +F VLLLERG VPYN   V++  G
Sbjct: 39  MRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMSHDG 98

Query: 61  LSNVFTNDDDGENPFQRFISEDGVENIRGWILGGGSMINAGFYSRAHKEFFEMQGMEWDM 120
                T+ ++ ++P Q FISE+GV N RG +LGG S INAGFYSRA K+FFE  G+ WD+
Sbjct: 99  FLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENSGLVWDL 158

Query: 121 EMVEQAYKCVEETVVSRPSLSSWQSAFRSALLESGVGPDNDFDLRNLVGTKIGGSIFGRE 180
             V Q+Y+ VE  +V RP L +WQ+A R ALLE GV P N F L + VGTKIGGS F R 
Sbjct: 159 SSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGSTFDRT 218

Query: 181 GNRHGAVELLNKANPVNLKVVVRAKVERII 211
           G RH + +LL  A   N++V V A VER++
Sbjct: 219 GRRHSSADLLRYARSSNIRVAVYATVERVL 248

BLAST of MC08g_new0561 vs. ExPASy Swiss-Prot
Match: O82784 ((R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 6.4e-58
Identity = 105/211 (49.76%), Postives = 150/211 (71.09%), Query Frame = 0

Query: 1   MKFVYDANNLPEKEEYDYIIIGGGTASCPLAVTISSNFSVLLLERGSVPYNFSTVLNIQG 60
           +KFVY+A +L  +  YDYII+GGGT+ CPLA T+S+N+SVL+LERG++   +   L + G
Sbjct: 40  LKFVYNAVDLELEGSYDYIIVGGGTSGCPLAATLSANYSVLVLERGTIATEYPNTLTVDG 99

Query: 61  LSNVFTNDDDGENPFQRFISEDGVENIRGWILGGGSMINAGFYSRAHKEFFEMQGMEWDM 120
            +      DDG+ P +RF+SEDG++N+R  ILGG ++INAG Y+RA++ F+   G+EWD+
Sbjct: 100 FAYNLQQQDDGKTPVERFVSEDGIDNVRSRILGGTTIINAGVYARANESFYNNSGVEWDL 159

Query: 121 EMVEQAYKCVEETVVSRPSLSSWQSAFRSALLESGVGPDNDFDLRNLVGTKIGGSIFGRE 180
           ++V +AY+ VE+ +V +PS  SWQS   +A LE+GV PDN F L +  GT++ GS F   
Sbjct: 160 DLVNEAYEWVEDAIVYKPSNQSWQSITGTAFLEAGVHPDNGFGLVHEEGTRLTGSTFDNS 219

Query: 181 GNRHGAVELLNKANPVNLKVVVRAKVERIIF 212
           G RH + ELLNK +P NLKV V A V++IIF
Sbjct: 220 GTRHASDELLNKGDPDNLKVAVEAAVQKIIF 250

BLAST of MC08g_new0561 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 3.2e-57
Identity = 104/211 (49.29%), Postives = 146/211 (69.19%), Query Frame = 0

Query: 1   MKFVYDANNLPEKEEYDYIIIGGGTASCPLAVTISSNFSVLLLERGSVPYNFSTVLNIQG 60
           + F YDA +L  +  YDY+I+GGGT+ CPLA T+S  + VL+LERGS+P  +  VL   G
Sbjct: 39  LSFAYDATDLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADG 98

Query: 61  LSNVFTNDDDGENPFQRFISEDGVENIRGWILGGGSMINAGFYSRAHKEFFEMQGMEWDM 120
                  +DDG+ P +RF+SEDG++N+RG +LGG S+INAG Y+RA+   +   G++WDM
Sbjct: 99  FVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSIINAGVYARANTSIYSASGVDWDM 158

Query: 121 EMVEQAYKCVEETVVSRPSLSSWQSAFRSALLESGVGPDNDFDLRNLVGTKIGGSIFGRE 180
           ++V Q Y+ VE+T+V +P+  SWQS  ++A LE+GV P++ F L +  GT+I GS F  +
Sbjct: 159 DLVNQTYEWVEDTIVYKPNSQSWQSVTKTAFLEAGVHPNHGFSLDHEEGTRITGSTFDNK 218

Query: 181 GNRHGAVELLNKANPVNLKVVVRAKVERIIF 212
           G RH A ELLNK N  NL+V V A VE+IIF
Sbjct: 219 GTRHAADELLNKGNSNNLRVGVHASVEKIIF 249

BLAST of MC08g_new0561 vs. NCBI nr
Match: XP_022153319.1 ((R)-mandelonitrile lyase-like [Momordica charantia])

HSP 1 Score: 429 bits (1103), Expect = 1.69e-146
Identity = 213/213 (100.00%), Postives = 213/213 (100.00%), Query Frame = 0

Query: 1   MKFVYDANNLPEKEEYDYIIIGGGTASCPLAVTISSNFSVLLLERGSVPYNFSTVLNIQG 60
           MKFVYDANNLPEKEEYDYIIIGGGTASCPLAVTISSNFSVLLLERGSVPYNFSTVLNIQG
Sbjct: 53  MKFVYDANNLPEKEEYDYIIIGGGTASCPLAVTISSNFSVLLLERGSVPYNFSTVLNIQG 112

Query: 61  LSNVFTNDDDGENPFQRFISEDGVENIRGWILGGGSMINAGFYSRAHKEFFEMQGMEWDM 120
           LSNVFTNDDDGENPFQRFISEDGVENIRGWILGGGSMINAGFYSRAHKEFFEMQGMEWDM
Sbjct: 113 LSNVFTNDDDGENPFQRFISEDGVENIRGWILGGGSMINAGFYSRAHKEFFEMQGMEWDM 172

Query: 121 EMVEQAYKCVEETVVSRPSLSSWQSAFRSALLESGVGPDNDFDLRNLVGTKIGGSIFGRE 180
           EMVEQAYKCVEETVVSRPSLSSWQSAFRSALLESGVGPDNDFDLRNLVGTKIGGSIFGRE
Sbjct: 173 EMVEQAYKCVEETVVSRPSLSSWQSAFRSALLESGVGPDNDFDLRNLVGTKIGGSIFGRE 232

Query: 181 GNRHGAVELLNKANPVNLKVVVRAKVERIIFFG 213
           GNRHGAVELLNKANPVNLKVVVRAKVERIIFFG
Sbjct: 233 GNRHGAVELLNKANPVNLKVVVRAKVERIIFFG 265

BLAST of MC08g_new0561 vs. NCBI nr
Match: KAA0067276.1 ((R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa] >TYK10890.1 (R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 313 bits (802), Expect = 3.42e-105
Identity = 151/214 (70.56%), Postives = 183/214 (85.51%), Query Frame = 0

Query: 1   MKFVYDANNLPEKEEYDYIIIGGGTASCPLAVTISSNFSVLLLERGSVPYNFSTVLNIQG 60
           MKFV++A +LP KEEYDYIIIGGGTA CPLA T+SS FS+L+LERGS P  + +VLN QG
Sbjct: 36  MKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKFSILVLERGSDPNKYPSVLNAQG 95

Query: 61  LSNVFTNDDDGENPFQRFISEDGVENIRGWILGGGSMINAGFYSRAHKEFFEMQGMEWDM 120
           ++N F   DDG+NPFQ F+SEDGVENIRG +LGGGSM+NAGFYSR H+EFFE  G++WDM
Sbjct: 96  INNAFAAGDDGKNPFQYFVSEDGVENIRGRVLGGGSMVNAGFYSRGHREFFENAGVDWDM 155

Query: 121 EMVEQAYKCVEETVVSRPSLSS-WQSAFRSALLESGVGPDNDFDLRNLVGTKIGGSIFGR 180
           E+VE+AY+ VEETVVS+P L++ WQ+AFRSALLE GV PDN FDLR+LVGTKIGGSIF  
Sbjct: 156 ELVEKAYEWVEETVVSQPILNNAWQAAFRSALLEGGVVPDNGFDLRHLVGTKIGGSIFDN 215

Query: 181 EGNRHGAVELLNKANPVNLKVVVRAKVERIIFFG 213
           +GNRHGAVELLNKANP N+KV ++A V++I+F G
Sbjct: 216 KGNRHGAVELLNKANPTNIKVAIQATVQKILFSG 249

BLAST of MC08g_new0561 vs. NCBI nr
Match: XP_022153313.1 ((R)-mandelonitrile lyase 1-like [Momordica charantia])

HSP 1 Score: 323 bits (828), Expect = 4.19e-105
Identity = 157/213 (73.71%), Postives = 182/213 (85.45%), Query Frame = 0

Query: 1   MKFVYDANNLPEKEEYDYIIIGGGTASCPLAVTISSNFSVLLLERGSVPYNFSTVLNIQG 60
           MKFV+DA +LPE  EYDYII+GGG A CPLA T+SS FS+L+LERGS P  F +VL  QG
Sbjct: 38  MKFVHDAKDLPETGEYDYIIVGGGAAGCPLATTLSSKFSILILERGSEPNKFPSVLTEQG 97

Query: 61  LSNVFTNDDDGENPFQRFISEDGVENIRGWILGGGSMINAGFYSRAHKEFFEMQGMEWDM 120
            S  FT +DDG+NPFQRF+SEDGVEN+RG +LGGG+MIN GFYSR H+EFF+  G+EWDM
Sbjct: 98  RSKAFTAEDDGKNPFQRFVSEDGVENLRGRVLGGGTMINGGFYSRGHREFFDTAGVEWDM 157

Query: 121 EMVEQAYKCVEETVVSRPSLSSWQSAFRSALLESGVGPDNDFDLRNLVGTKIGGSIFGRE 180
           E+VE+AY+ VEETVVSRP+LS+WQSAFRSALLESGV PDN FDLR+LVGT+IGGS F  +
Sbjct: 158 ELVEKAYQWVEETVVSRPNLSAWQSAFRSALLESGVLPDNGFDLRHLVGTRIGGSTFDGK 217

Query: 181 GNRHGAVELLNKANPVNLKVVVRAKVERIIFFG 213
           GNRHGAVELLNKANP NLKVV+RA VERIIF G
Sbjct: 218 GNRHGAVELLNKANPANLKVVIRATVERIIFSG 250

BLAST of MC08g_new0561 vs. NCBI nr
Match: TYK10894.1 ((R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 315 bits (807), Expect = 1.93e-104
Identity = 153/213 (71.83%), Postives = 180/213 (84.51%), Query Frame = 0

Query: 1   MKFVYDANNLPEKEEYDYIIIGGGTASCPLAVTISSNFSVLLLERGSVPYNFSTVLNIQG 60
           MKFV++A +LP KEEYDYIIIGGGTA CPLA T+SS FSVLLLERGS P  + +VLN QG
Sbjct: 36  MKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQG 95

Query: 61  LSNVFTNDDDGENPFQRFISEDGVENIRGWILGGGSMINAGFYSRAHKEFFEMQGMEWDM 120
           L NVF   DDG+NPFQRF+SEDG+ENIRG +LGGGSM+NAGFYSR H+EFF   G++W+M
Sbjct: 96  LLNVFAAGDDGKNPFQRFVSEDGIENIRGRVLGGGSMVNAGFYSRGHQEFFASAGVDWNM 155

Query: 121 EMVEQAYKCVEETVVSRPSLSSWQSAFRSALLESGVGPDNDFDLRNLVGTKIGGSIFGRE 180
           E+VE+AY+ VEETVVS+P L++WQSAFRS+LLE GV PDN FDLR+LVGTK GGSIF  +
Sbjct: 156 ELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVIPDNGFDLRHLVGTKTGGSIFDNK 215

Query: 181 GNRHGAVELLNKANPVNLKVVVRAKVERIIFFG 213
           GNRHGAVELLNKANP N+KV   A V+RI+F G
Sbjct: 216 GNRHGAVELLNKANPTNIKVATEATVQRILFSG 248

BLAST of MC08g_new0561 vs. NCBI nr
Match: XP_038889699.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 321 bits (822), Expect = 3.17e-104
Identity = 158/213 (74.18%), Postives = 181/213 (84.98%), Query Frame = 0

Query: 1   MKFVYDANNLPEKEEYDYIIIGGGTASCPLAVTISSNFSVLLLERGSVPYNFSTVLNIQG 60
           +KFV++AN LPE  EYDYIIIGGGTA CPLA T+SS +SVLLLERGS P  + +VLN QG
Sbjct: 35  LKFVHEANELPETGEYDYIIIGGGTAGCPLAATLSSKYSVLLLERGSDPNKYPSVLNEQG 94

Query: 61  LSNVFTNDDDGENPFQRFISEDGVENIRGWILGGGSMINAGFYSRAHKEFFEMQGMEWDM 120
           L N F  +DDG+NPFQ FISEDGVENIRG +LGGGSMINAGFYSR H+EFFE  G++WDM
Sbjct: 95  LLNAFVAEDDGQNPFQHFISEDGVENIRGRVLGGGSMINAGFYSRGHREFFETAGVDWDM 154

Query: 121 EMVEQAYKCVEETVVSRPSLSSWQSAFRSALLESGVGPDNDFDLRNLVGTKIGGSIFGRE 180
           E+VE+AY+ VE+TVVSRPSLS+WQSAFR ALLE+GVGPDN FDL++LVGTK GGSIF  +
Sbjct: 155 ELVEKAYQWVEQTVVSRPSLSAWQSAFRGALLEAGVGPDNGFDLKHLVGTKTGGSIFDNK 214

Query: 181 GNRHGAVELLNKANPVNLKVVVRAKVERIIFFG 213
           GNRHGAVELLNKA P NLKV  +A VERIIF G
Sbjct: 215 GNRHGAVELLNKAEPQNLKVGTQAIVERIIFTG 247

BLAST of MC08g_new0561 vs. ExPASy TrEMBL
Match: A0A6J1DIQ5 ((R)-mandelonitrile lyase-like OS=Momordica charantia OX=3673 GN=LOC111020841 PE=3 SV=1)

HSP 1 Score: 429 bits (1103), Expect = 8.17e-147
Identity = 213/213 (100.00%), Postives = 213/213 (100.00%), Query Frame = 0

Query: 1   MKFVYDANNLPEKEEYDYIIIGGGTASCPLAVTISSNFSVLLLERGSVPYNFSTVLNIQG 60
           MKFVYDANNLPEKEEYDYIIIGGGTASCPLAVTISSNFSVLLLERGSVPYNFSTVLNIQG
Sbjct: 53  MKFVYDANNLPEKEEYDYIIIGGGTASCPLAVTISSNFSVLLLERGSVPYNFSTVLNIQG 112

Query: 61  LSNVFTNDDDGENPFQRFISEDGVENIRGWILGGGSMINAGFYSRAHKEFFEMQGMEWDM 120
           LSNVFTNDDDGENPFQRFISEDGVENIRGWILGGGSMINAGFYSRAHKEFFEMQGMEWDM
Sbjct: 113 LSNVFTNDDDGENPFQRFISEDGVENIRGWILGGGSMINAGFYSRAHKEFFEMQGMEWDM 172

Query: 121 EMVEQAYKCVEETVVSRPSLSSWQSAFRSALLESGVGPDNDFDLRNLVGTKIGGSIFGRE 180
           EMVEQAYKCVEETVVSRPSLSSWQSAFRSALLESGVGPDNDFDLRNLVGTKIGGSIFGRE
Sbjct: 173 EMVEQAYKCVEETVVSRPSLSSWQSAFRSALLESGVGPDNDFDLRNLVGTKIGGSIFGRE 232

Query: 181 GNRHGAVELLNKANPVNLKVVVRAKVERIIFFG 213
           GNRHGAVELLNKANPVNLKVVVRAKVERIIFFG
Sbjct: 233 GNRHGAVELLNKANPVNLKVVVRAKVERIIFFG 265

BLAST of MC08g_new0561 vs. ExPASy TrEMBL
Match: A0A0A0LJ80 (GMC_OxRdtase_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G055020 PE=3 SV=1)

HSP 1 Score: 314 bits (805), Expect = 5.79e-106
Identity = 153/213 (71.83%), Postives = 178/213 (83.57%), Query Frame = 0

Query: 1   MKFVYDANNLPEKEEYDYIIIGGGTASCPLAVTISSNFSVLLLERGSVPYNFSTVLNIQG 60
           MKFV++A +LP KEEYDYIIIGGGTA CPLA T+SS FSVLLLERGS P  + +VLN +G
Sbjct: 37  MKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEKG 96

Query: 61  LSNVFTNDDDGENPFQRFISEDGVENIRGWILGGGSMINAGFYSRAHKEFFEMQGMEWDM 120
           LS  F  +DDG+ PFQRF+SEDGVENIRG ILGGGSM+NAGFYSR HKEFFE  G++WDM
Sbjct: 97  LSKAFDTEDDGKTPFQRFVSEDGVENIRGRILGGGSMVNAGFYSRGHKEFFESAGVDWDM 156

Query: 121 EMVEQAYKCVEETVVSRPSLSSWQSAFRSALLESGVGPDNDFDLRNLVGTKIGGSIFGRE 180
           E+VE+AY+ +EE+VVS+P L  WQSAFRSALLE G+ PDN FDLR+LVGTK GGSI   +
Sbjct: 157 ELVEKAYEWIEESVVSQPILKDWQSAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIIDDK 216

Query: 181 GNRHGAVELLNKANPVNLKVVVRAKVERIIFFG 213
           GNRHGAVELLNKANP NLKV + A V+RI+F G
Sbjct: 217 GNRHGAVELLNKANPKNLKVAIEATVQRILFSG 249

BLAST of MC08g_new0561 vs. ExPASy TrEMBL
Match: A0A5D3CHQ2 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00620 PE=3 SV=1)

HSP 1 Score: 313 bits (802), Expect = 1.66e-105
Identity = 151/214 (70.56%), Postives = 183/214 (85.51%), Query Frame = 0

Query: 1   MKFVYDANNLPEKEEYDYIIIGGGTASCPLAVTISSNFSVLLLERGSVPYNFSTVLNIQG 60
           MKFV++A +LP KEEYDYIIIGGGTA CPLA T+SS FS+L+LERGS P  + +VLN QG
Sbjct: 36  MKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKFSILVLERGSDPNKYPSVLNAQG 95

Query: 61  LSNVFTNDDDGENPFQRFISEDGVENIRGWILGGGSMINAGFYSRAHKEFFEMQGMEWDM 120
           ++N F   DDG+NPFQ F+SEDGVENIRG +LGGGSM+NAGFYSR H+EFFE  G++WDM
Sbjct: 96  INNAFAAGDDGKNPFQYFVSEDGVENIRGRVLGGGSMVNAGFYSRGHREFFENAGVDWDM 155

Query: 121 EMVEQAYKCVEETVVSRPSLSS-WQSAFRSALLESGVGPDNDFDLRNLVGTKIGGSIFGR 180
           E+VE+AY+ VEETVVS+P L++ WQ+AFRSALLE GV PDN FDLR+LVGTKIGGSIF  
Sbjct: 156 ELVEKAYEWVEETVVSQPILNNAWQAAFRSALLEGGVVPDNGFDLRHLVGTKIGGSIFDN 215

Query: 181 EGNRHGAVELLNKANPVNLKVVVRAKVERIIFFG 213
           +GNRHGAVELLNKANP N+KV ++A V++I+F G
Sbjct: 216 KGNRHGAVELLNKANPTNIKVAIQATVQKILFSG 249

BLAST of MC08g_new0561 vs. ExPASy TrEMBL
Match: A0A6J1DH55 ((R)-mandelonitrile lyase 1-like OS=Momordica charantia OX=3673 GN=LOC111020834 PE=3 SV=1)

HSP 1 Score: 323 bits (828), Expect = 2.03e-105
Identity = 157/213 (73.71%), Postives = 182/213 (85.45%), Query Frame = 0

Query: 1   MKFVYDANNLPEKEEYDYIIIGGGTASCPLAVTISSNFSVLLLERGSVPYNFSTVLNIQG 60
           MKFV+DA +LPE  EYDYII+GGG A CPLA T+SS FS+L+LERGS P  F +VL  QG
Sbjct: 38  MKFVHDAKDLPETGEYDYIIVGGGAAGCPLATTLSSKFSILILERGSEPNKFPSVLTEQG 97

Query: 61  LSNVFTNDDDGENPFQRFISEDGVENIRGWILGGGSMINAGFYSRAHKEFFEMQGMEWDM 120
            S  FT +DDG+NPFQRF+SEDGVEN+RG +LGGG+MIN GFYSR H+EFF+  G+EWDM
Sbjct: 98  RSKAFTAEDDGKNPFQRFVSEDGVENLRGRVLGGGTMINGGFYSRGHREFFDTAGVEWDM 157

Query: 121 EMVEQAYKCVEETVVSRPSLSSWQSAFRSALLESGVGPDNDFDLRNLVGTKIGGSIFGRE 180
           E+VE+AY+ VEETVVSRP+LS+WQSAFRSALLESGV PDN FDLR+LVGT+IGGS F  +
Sbjct: 158 ELVEKAYQWVEETVVSRPNLSAWQSAFRSALLESGVLPDNGFDLRHLVGTRIGGSTFDGK 217

Query: 181 GNRHGAVELLNKANPVNLKVVVRAKVERIIFFG 213
           GNRHGAVELLNKANP NLKVV+RA VERIIF G
Sbjct: 218 GNRHGAVELLNKANPANLKVVIRATVERIIFSG 250

BLAST of MC08g_new0561 vs. ExPASy TrEMBL
Match: A0A5D3CI96 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00680 PE=3 SV=1)

HSP 1 Score: 315 bits (807), Expect = 9.33e-105
Identity = 153/213 (71.83%), Postives = 180/213 (84.51%), Query Frame = 0

Query: 1   MKFVYDANNLPEKEEYDYIIIGGGTASCPLAVTISSNFSVLLLERGSVPYNFSTVLNIQG 60
           MKFV++A +LP KEEYDYIIIGGGTA CPLA T+SS FSVLLLERGS P  + +VLN QG
Sbjct: 36  MKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQG 95

Query: 61  LSNVFTNDDDGENPFQRFISEDGVENIRGWILGGGSMINAGFYSRAHKEFFEMQGMEWDM 120
           L NVF   DDG+NPFQRF+SEDG+ENIRG +LGGGSM+NAGFYSR H+EFF   G++W+M
Sbjct: 96  LLNVFAAGDDGKNPFQRFVSEDGIENIRGRVLGGGSMVNAGFYSRGHQEFFASAGVDWNM 155

Query: 121 EMVEQAYKCVEETVVSRPSLSSWQSAFRSALLESGVGPDNDFDLRNLVGTKIGGSIFGRE 180
           E+VE+AY+ VEETVVS+P L++WQSAFRS+LLE GV PDN FDLR+LVGTK GGSIF  +
Sbjct: 156 ELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVIPDNGFDLRHLVGTKTGGSIFDNK 215

Query: 181 GNRHGAVELLNKANPVNLKVVVRAKVERIIFFG 213
           GNRHGAVELLNKANP N+KV   A V+RI+F G
Sbjct: 216 GNRHGAVELLNKANPTNIKVATEATVQRILFSG 248

BLAST of MC08g_new0561 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 226.5 bits (576), Expect = 2.1e-59
Identity = 111/210 (52.86%), Postives = 144/210 (68.57%), Query Frame = 0

Query: 1   MKFVYDANNLPEKEEYDYIIIGGGTASCPLAVTISSNFSVLLLERGSVPYNFSTVLNIQG 60
           M+F+ +A +   ++ YDYII+GGGTA CPLA T+S +F VLLLERG VPYN   V++  G
Sbjct: 39  MRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMSHDG 98

Query: 61  LSNVFTNDDDGENPFQRFISEDGVENIRGWILGGGSMINAGFYSRAHKEFFEMQGMEWDM 120
                T+ ++ ++P Q FISE+GV N RG +LGG S INAGFYSRA K+FFE  G+ WD+
Sbjct: 99  FLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENSGLVWDL 158

Query: 121 EMVEQAYKCVEETVVSRPSLSSWQSAFRSALLESGVGPDNDFDLRNLVGTKIGGSIFGRE 180
             V Q+Y+ VE  +V RP L +WQ+A R ALLE GV P N F L + VGTKIGGS F R 
Sbjct: 159 SSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGSTFDRT 218

Query: 181 GNRHGAVELLNKANPVNLKVVVRAKVERII 211
           G RH + +LL  A   N++V V A VER++
Sbjct: 219 GRRHSSADLLRYARSSNIRVAVYATVERVL 248

BLAST of MC08g_new0561 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 193.7 bits (491), Expect = 1.5e-49
Identity = 99/209 (47.37%), Postives = 132/209 (63.16%), Query Frame = 0

Query: 3   FVYDANNLPEKEEYDYIIIGGGTASCPLAVTISSNFSVLLLERGSVPYNFSTVLNIQGLS 62
           F+ DA   P    YDYIIIGGGTA CPLA T+S N SVLLLERG  PYN   +  +    
Sbjct: 33  FMRDATGSPTTSYYDYIIIGGGTAGCPLAATLSQNASVLLLERGDSPYNNPNITRLSAFG 92

Query: 63  NVFTNDDDGENPFQRFISEDGVENIRGWILGGGSMINAGFYSRAHKEFFEMQGMEWDMEM 122
               +D    +P QRF+SEDGV N R  +LGGGS +NAGFY+RA  ++  ++ M WD  +
Sbjct: 93  AAL-SDLSESSPSQRFVSEDGVINARARVLGGGSALNAGFYTRAGTKY--VRNMGWDGAL 152

Query: 123 VEQAYKCVEETVVSRPSLSSWQSAFRSALLESGVGPDNDFDLRNLVGTKIGGSIFGREGN 182
             ++Y+ VE  V  +P +  WQ+A R  LLE+G+ P+N F   ++ GTK GG+IF R GN
Sbjct: 153 ANESYQWVEAKVAFQPPMGRWQTAVRDGLLEAGIVPNNGFTYDHINGTKFGGTIFDRNGN 212

Query: 183 RHGAVELLNKANPVNLKVVVRAKVERIIF 212
           RH A +LL  A+P  + V++ A V RI+F
Sbjct: 213 RHTAADLLEYADPKGITVLLHATVHRILF 238

BLAST of MC08g_new0561 vs. TAIR 10
Match: AT3G56060.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 192.6 bits (488), Expect = 3.3e-49
Identity = 103/210 (49.05%), Postives = 135/210 (64.29%), Query Frame = 0

Query: 2   KFVYDANNLPEKEEYDYIIIGGGTASCPLAVTISSNFSVLLLERGSVPYNFSTVLNIQGL 61
           +F+ DA   P+   +DYIIIGGGTA C LA T+S N +VL+LERG  PY+     +I   
Sbjct: 32  RFMKDATLAPKLSHFDYIIIGGGTAGCALAATLSQNATVLVLERGGSPYDDPAATDIGNF 91

Query: 62  SNVFTNDDDGENPFQRFISEDGVENIRGWILGGGSMINAGFYSRAHKEFFEMQGMEWDME 121
           +N   N     +  Q FISEDGV N R  +LGGG++INAGFYSRA ++F    G  W+ +
Sbjct: 92  ANTLLNITP-NSWSQLFISEDGVFNSRARVLGGGTVINAGFYSRAEEDFVAEAG--WERD 151

Query: 122 MVEQAYKCVEETVVSRPSLSSWQSAFRSALLESGVGPDNDFDLRNLVGTKIGGSIFGREG 181
            VE AY+ VE+ VV  P ++ WQSAFR  LLE+GV P N F   ++VGTK GG+IF R+G
Sbjct: 152 EVEAAYEWVEKKVVFEPPVNKWQSAFRDGLLEAGVTPYNGFTYEHIVGTKFGGTIFDRDG 211

Query: 182 NRHGAVELLNKANPVNLKVVVRAKVERIIF 212
           +RH A  LL  ANP  + V + A V +I+F
Sbjct: 212 HRHTAANLLEYANPNMIVVYLHASVHKILF 238

BLAST of MC08g_new0561 vs. TAIR 10
Match: AT5G51930.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 191.4 bits (485), Expect = 7.3e-49
Identity = 102/209 (48.80%), Postives = 131/209 (62.68%), Query Frame = 0

Query: 3   FVYDANNLPEKEEYDYIIIGGGTASCPLAVTISSNFSVLLLERGSVPYNFSTVLNIQGLS 62
           F+ DA   P+   +DYIIIGGGTA C LA T+S N SVL+LERG  PY   T  ++    
Sbjct: 60  FMKDATLAPKNASFDYIIIGGGTAGCALAATLSQNASVLVLERGGSPYENPTATDMGNSV 119

Query: 63  NVFTNDDDGENPFQRFISEDGVENIRGWILGGGSMINAGFYSRAHKEFFEMQGMEWDMEM 122
           N   N+    +  Q FISEDGV N R  +LGGGS+IN GFYSRA  ++ E    EW+ME 
Sbjct: 120 NTLLNNTP-NSWSQLFISEDGVYNTRPRVLGGGSVINGGFYSRAGNDYVE--EAEWEMEE 179

Query: 123 VEQAYKCVEETVVSRPSLSSWQSAFRSALLESGVGPDNDFDLRNLVGTKIGGSIFGREGN 182
           VE AY+ VE+ +V  P +  WQ AF+  LLE+G  PDN F   ++ GTKIGG+IF R G+
Sbjct: 180 VEAAYEWVEKKLVFEPQVIEWQKAFKDGLLEAGESPDNGFTYDHIYGTKIGGTIFDRAGH 239

Query: 183 RHGAVELLNKANPVNLKVVVRAKVERIIF 212
           RH A  LL  ANP  + V + A V +++F
Sbjct: 240 RHTAANLLEYANPNRIVVYLHASVHKVLF 265

BLAST of MC08g_new0561 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 189.9 bits (481), Expect = 2.1e-48
Identity = 99/200 (49.50%), Postives = 131/200 (65.50%), Query Frame = 0

Query: 12  EKEEYDYIIIGGGTASCPLAVTISSNFSVLLLERGSVPYNFSTVLNIQGLSNVFTNDDDG 71
           +   YDYI+IGGGTA CPLA T+S NFSVL+LERG VP+  + V  ++   ++   D   
Sbjct: 59  QDSSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRNF-HIGLADISA 118

Query: 72  ENPFQRFISEDGVENIRGWILGGGSMINAGFYSRAHKEFFEMQGMEWDMEMVEQAYKCVE 131
            +  Q F+S DGV N R  +LGGGS INAGFYSRA   F +  G  WD ++V+++Y  VE
Sbjct: 119 SSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYPWVE 178

Query: 132 ETVVSRPSLSSWQSAFRSALLESGVGPDNDFDLRNLVGTKIGGSIFGREGNRHGAVELLN 191
             +V +P L+ WQ A R +LLE GV P N F   ++ GTKIGG+IF R G RH A ELL 
Sbjct: 179 REIVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLA 238

Query: 192 KANPVNLKVVVRAKVERIIF 212
            ANP  L+V++ A V++I+F
Sbjct: 239 YANPQKLRVLIYATVQKIVF 255

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P527074.5e-5950.71(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1[more]
O500489.9e-5950.71(R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1[more]
Q9SSM22.9e-5852.86(R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 ... [more]
O827846.4e-5849.76(R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1[more]
Q945K23.2e-5749.29(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022153319.11.69e-146100.00(R)-mandelonitrile lyase-like [Momordica charantia][more]
KAA0067276.13.42e-10570.56(R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa] >TYK10890.1 (R)-mande... [more]
XP_022153313.14.19e-10573.71(R)-mandelonitrile lyase 1-like [Momordica charantia][more]
TYK10894.11.93e-10471.83(R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa][more]
XP_038889699.13.17e-10474.18(R)-mandelonitrile lyase 1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1DIQ58.17e-147100.00(R)-mandelonitrile lyase-like OS=Momordica charantia OX=3673 GN=LOC111020841 PE=... [more]
A0A0A0LJ805.79e-10671.83GMC_OxRdtase_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G055... [more]
A0A5D3CHQ21.66e-10570.56(R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A6J1DH552.03e-10573.71(R)-mandelonitrile lyase 1-like OS=Momordica charantia OX=3673 GN=LOC111020834 P... [more]
A0A5D3CI969.33e-10571.83(R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
Match NameE-valueIdentityDescription
AT1G73050.12.1e-5952.86Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G12570.11.5e-4947.37Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT3G56060.13.3e-4949.05Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51930.17.3e-4948.80Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G72970.12.1e-4849.50Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 16..211
e-value: 1.6E-11
score: 44.2
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 1..214
e-value: 1.4E-45
score: 157.7
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 5..211
NoneNo IPR availablePANTHERPTHR45968:SF23(R)-MANDELONITRILE LYASE 4-RELATEDcoord: 1..211
NoneNo IPR availablePANTHERPTHR45968OSJNBA0019K04.7 PROTEINcoord: 1..211
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..28
score: 5.0

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g_new0561.1MC08g_new0561.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors