MC08g_new0537 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g_new0537
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Ribonuclease H
Location	MC08: 28398187 .. 28398965 (+)
RNA-Seq Expression	MC08g_new0537
Synteny	MC08g_new0537

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CCGCTTCGAGGGAGTGTTATGCCTCCGCACTCAAAGGATCATCGGTATGCGCCCTCGAAACTCACGCCAGTGGGAAGGGGACACCCGAGTTCAAGGCCGACCTGGCAAGAAGGGAGTTTTCCGCGCCCACTGAGGAGCTCGAGCTTGTTCCTCTGCTTAGTCCCGAGAAGCAGGTAAGCATAGGAACTAAGTTGGGGGCCACCGACAGAGAGGAGCTAATCCACTTCCTCAGATCCAACTCGGACGTCTTCGCATGGTCTCACGAGGACATGCCCGGTATCGACCCGGAGATTATGACGCATCGCCTCAGCATAGATCCGTCATTCCGACCTGTGAAACAAAAACGAAGACCTATAAACAAGGAGAGAAGTGATGTAATTGTTGAGGAAGTTAACAAACTTTTGAAAGCTGAATACATAAGAGAGGGGGCCAATGTTTGGGGAAGAGAATACCCTGGACACGTGGAGGACCGCTATTGGTGCTCCAAGATGACTCATGAGTCATGCCCAGCTCGAACCGCCGAGATCACCACCAGTAGCGTTGACAGGTGCTAAGTATGAGGGCCGAGGTGCGACCTGGCCAAGGTCCGATCTACCGGGAAGCTCGGTGGGGGCCGATGTGAGCTACTGTCAGCGGCGTGATTATCGGGTGTAACCGTCGGTCCCGAGATTACCGGGTGTAACCGTCGCGAGATTAACGGGACTACCACCCATAAGTAGAGGACCTCACTCCACGATCAGGTACGATGATTTTTGACCTCGAACTAAAACTGGGAATCC

mRNA sequence

ATGCCCGGTATCGACCCGGAGATTATGACGCATCGCCTCAGCATAGATCCGTCATTCCGACCTGTGAAACAAAAACGAAGACCTATAAACAAGGAGAGAAGTGATGTAATTGTTGAGGAAGTTAACAAACTTTTGAAAGCTGAATACATAAGAGAGGGGGCCAATGTTTGGGGAAGAGAATACCCTGGACACGTGGAGGACCGCTATTGGTGCTCCAAGATGACTCATGAGTCATGCCCAGCTCGAACCGCCGAGATCACCACCAGTAGCGTTGACAGGTGCTAA

Coding sequence (CDS)

ATGCCCGGTATCGACCCGGAGATTATGACGCATCGCCTCAGCATAGATCCGTCATTCCGACCTGTGAAACAAAAACGAAGACCTATAAACAAGGAGAGAAGTGATGTAATTGTTGAGGAAGTTAACAAACTTTTGAAAGCTGAATACATAAGAGAGGGGGCCAATGTTTGGGGAAGAGAATACCCTGGACACGTGGAGGACCGCTATTGGTGCTCCAAGATGACTCATGAGTCATGCCCAGCTCGAACCGCCGAGATCACCACCAGTAGCGTTGACAGGTGCTAA

Protein sequence

MPGIDPEIMTHRLSIDPSFRPVKQKRRPINKERSDVIVEEVNKLLKAEYIREGANVWGREYPGHVEDRYWCSKMTHESCPARTAEITTSSVDRC

Homology

[Close]

BLAST of MC08g_new0537 vs. NCBI nr
Match: XP_022136647.1 (uncharacterized protein LOC111008302 [Momordica charantia])

HSP 1 Score: 79.0 bits (193), Expect = 1.24e-14
Identity = 38/40 (95.00%), Postives = 39/40 (97.50%), Query Frame = 0


Query: 1   MPGIDPEIMTHRLSIDPSFRPVKQKRRPINKERSDVIVEE 40
           MP IDP+IMTHRLSIDPSFRPVKQKRRPINKERSDVIVEE
Sbjct: 71  MPSIDPKIMTHRLSIDPSFRPVKQKRRPINKERSDVIVEE 110

[Close]

BLAST of MC08g_new0537 vs. NCBI nr
Match: XP_022154603.1 (uncharacterized protein LOC111021825 [Momordica charantia])

HSP 1 Score: 75.1 bits (183), Expect = 2.78e-13
Identity = 34/52 (65.38%), Postives = 44/52 (84.62%), Query Frame = 0


Query: 1  MPGIDPEIMTHRLSIDPSFRPVKQKRRPINKERSDVIVEEVNKLLKAEYIRE 52
          MPGIDP I+ HRL +DPS+RPV+QKRRP + ERS+VI +EV +LL+A +IRE
Sbjct: 1  MPGIDPSIIVHRLRVDPSYRPVRQKRRPFDAERSNVICKEVEQLLRANFIRE 52

[Close]

BLAST of MC08g_new0537 vs. NCBI nr
Match: XP_010496846.1 (PREDICTED: protein NYNRIN-like [Camelina sativa])

HSP 1 Score: 68.9 bits (167), Expect = 4.20e-11
Identity = 30/52 (57.69%), Postives = 43/52 (82.69%), Query Frame = 0


Query: 1  MPGIDPEIMTHRLSIDPSFRPVKQKRRPINKERSDVIVEEVNKLLKAEYIRE 52
          MPGI PE+++H L++DP+FRPVKQKRR +  ER++++ +EV +LLKA  IRE
Sbjct: 11 MPGISPEVISHELNVDPTFRPVKQKRRKLGPERAEIVNKEVERLLKAGQIRE 62

[Close]

BLAST of MC08g_new0537 vs. NCBI nr
Match: XP_019089153.1 (PREDICTED: uncharacterized protein LOC109127968 [Camelina sativa])

HSP 1 Score: 68.9 bits (167), Expect = 4.24e-11
Identity = 30/52 (57.69%), Postives = 43/52 (82.69%), Query Frame = 0


Query: 1  MPGIDPEIMTHRLSIDPSFRPVKQKRRPINKERSDVIVEEVNKLLKAEYIRE 52
          MPGI PE+++H L++DP+FRPVKQKRR +  ER++++ +EV +LLKA  IRE
Sbjct: 11 MPGISPEVISHELNVDPTFRPVKQKRRKLGPERAEIVNKEVERLLKAGQIRE 62

[Close]

BLAST of MC08g_new0537 vs. NCBI nr
Match: KAG7585529.1 (P-loop containing nucleoside triphosphate hydrolase [Arabidopsis thaliana x Arabidopsis arenosa])

HSP 1 Score: 68.2 bits (165), Expect = 8.10e-11
Identity = 32/52 (61.54%), Postives = 42/52 (80.77%), Query Frame = 0


Query: 1    MPGIDPEIMTHRLSIDPSFRPVKQKRRPINKERSDVIVEEVNKLLKAEYIRE 52
            MPGIDP I+ H+L++DPSF+PVKQKRR +  ER+ V+ +EV+KLLK   IRE
Sbjct: 1958 MPGIDPSIICHKLNVDPSFKPVKQKRRKLGVERAKVVNDEVDKLLKIGSIRE 2009

[Close]

BLAST of MC08g_new0537 vs. ExPASy TrEMBL
Match: A0A6J1C617 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111008302 PE=4 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 6.00e-15
Identity = 38/40 (95.00%), Postives = 39/40 (97.50%), Query Frame = 0


Query: 1   MPGIDPEIMTHRLSIDPSFRPVKQKRRPINKERSDVIVEE 40
           MP IDP+IMTHRLSIDPSFRPVKQKRRPINKERSDVIVEE
Sbjct: 71  MPSIDPKIMTHRLSIDPSFRPVKQKRRPINKERSDVIVEE 110

[Close]

BLAST of MC08g_new0537 vs. ExPASy TrEMBL
Match: A0A6J1DKR2 (uncharacterized protein LOC111021825 OS=Momordica charantia OX=3673 GN=LOC111021825 PE=4 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.34e-13
Identity = 34/52 (65.38%), Postives = 44/52 (84.62%), Query Frame = 0


Query: 1  MPGIDPEIMTHRLSIDPSFRPVKQKRRPINKERSDVIVEEVNKLLKAEYIRE 52
          MPGIDP I+ HRL +DPS+RPV+QKRRP + ERS+VI +EV +LL+A +IRE
Sbjct: 1  MPGIDPSIIVHRLRVDPSYRPVRQKRRPFDAERSNVICKEVEQLLRANFIRE 52

[Close]

BLAST of MC08g_new0537 vs. ExPASy TrEMBL
Match: A0A2N9EKX3 (Retrotrans_gag domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS3317 PE=4 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 4.28e-12
Identity = 33/52 (63.46%), Postives = 43/52 (82.69%), Query Frame = 0


Query: 1   MPGIDPEIMTHRLSIDPSFRPVKQKRRPINKERSDVIVEEVNKLLKAEYIRE 52
           MPGIDP I++H+L++DPS RPVKQKRR    ER++ I+EEV+KLL A +IRE
Sbjct: 708 MPGIDPSIISHKLNVDPSLRPVKQKRRVFAPERNNAIMEEVDKLLAANFIRE 759

[Close]

BLAST of MC08g_new0537 vs. ExPASy TrEMBL
Match: A0A2N9FH63 (Reverse transcriptase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS14444 PE=4 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 5.97e-12
Identity = 32/52 (61.54%), Postives = 43/52 (82.69%), Query Frame = 0


Query: 1   MPGIDPEIMTHRLSIDPSFRPVKQKRRPINKERSDVIVEEVNKLLKAEYIRE 52
           MPGIDP I++H+L++DPS RP+KQKRR    ER++ I+EEV+KLL A +IRE
Sbjct: 738 MPGIDPSIISHKLNVDPSLRPIKQKRRVFAPERNNAIMEEVDKLLAANFIRE 789

[Close]

BLAST of MC08g_new0537 vs. ExPASy TrEMBL
Match: A0A2N9FG08 (Ribonuclease H OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS14044 PE=4 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 8.17e-12
Identity = 31/52 (59.62%), Postives = 43/52 (82.69%), Query Frame = 0


Query: 1   MPGIDPEIMTHRLSIDPSFRPVKQKRRPINKERSDVIVEEVNKLLKAEYIRE 52
           MPGIDP +++H+L++DPS RP+KQKRR    ER++ I+EEV+KLL A +IRE
Sbjct: 766 MPGIDPSVISHKLNVDPSLRPIKQKRRVFAPERNNAIMEEVDKLLAANFIRE 817

The following BLAST results are available for this feature:

BLAST of MC08g_new0537 vs.


Match Name	E-value	Identity	Description

BLAST of MC08g_new0537 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022136647.1	1.24e-14	95.00	uncharacterized protein LOC111008302 [Momordica charantia]	[more]
XP_022154603.1	2.78e-13	65.38	uncharacterized protein LOC111021825 [Momordica charantia]	[more]
XP_010496846.1	4.20e-11	57.69	PREDICTED: protein NYNRIN-like [Camelina sativa]	[more]
XP_019089153.1	4.24e-11	57.69	PREDICTED: uncharacterized protein LOC109127968 [Camelina sativa]	[more]
KAG7585529.1	8.10e-11	61.54	P-loop containing nucleoside triphosphate hydrolase [Arabidopsis thaliana x Arab...	[more]

BLAST of MC08g_new0537 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C617	6.00e-15	95.00	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111008302 PE=4 SV=1	[more]
A0A6J1DKR2	1.34e-13	65.38	uncharacterized protein LOC111021825 OS=Momordica charantia OX=3673 GN=LOC111021...	[more]
A0A2N9EKX3	4.28e-12	63.46	Retrotrans_gag domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCU...	[more]
A0A2N9FH63	5.97e-12	61.54	Reverse transcriptase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS14444 PE=4 SV=1	[more]
A0A2N9FG08	8.17e-12	59.62	Ribonuclease H OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS14044 PE=4 SV=1	[more]

BLAST of MC08g_new0537 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.10.10	HIV Type 1 Reverse Transcriptase, subunit A, domain 1	coord: 3..61 e-value: 5.5E-7 score: 31.6
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 8..52

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g_new0537.1	MC08g_new0537.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0030430	host cell cytoplasm
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity