MC08g_new0200 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g_new0200 vs. ExPASy Swiss-Prot
Match: Q9LY84 (GDSL esterase/lipase At5g14450 OS=Arabidopsis thaliana OX=3702 GN=At5g14450 PE=2 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 9.8e-126
Identity = 214/381 (56.17%), Postives = 277/381 (72.70%), Query Frame = 0

Query: 2   KIQMAFTV-AWILLS--AAWISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGE 61
           K+ ++ TV +W+LL   A   S++    C FPAIYNFGDSNSDTGGISAAF P   P G+
Sbjct: 9   KLMVSSTVFSWLLLCLFAVTTSVSVQPTCTFPAIYNFGDSNSDTGGISAAFEPIRDPYGQ 68

Query: 62  TFFHKAAGRGCDGRHIIDFIANHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESV 121
            FFH+  GR  DGR  IDFIA  L LPYLSAYLNS+G+NFRHGANFATGGSTIRRQNE++
Sbjct: 69  GFFHRPTGRDSDGRLTIDFIAERLGLPYLSAYLNSLGSNFRHGANFATGGSTIRRQNETI 128

Query: 122 FLNGASPFSLDIQVVQFRQFKNRTIDLYREATAEKSIRSTLPVPEDFSKALFTMDIGQND 181
           F  G SPFSLD+Q+ QF QFK R+  L+ +  +    R  LP  E+F+KAL+T DIGQND
Sbjct: 129 FQYGISPFSLDMQIAQFDQFKARSALLFTQIKSRYD-REKLPRQEEFAKALYTFDIGQND 188

Query: 182 LVAGFRKMTNEQLRSVIPDIIGEFATAVEDLYKEGARAFWVHNSGPIGCIPV---AIRSS 241
           L  GFR M+ +QL++ IPDI+   A+AV ++Y++G R FWVHN+GP GC+PV    + + 
Sbjct: 189 LSVGFRTMSVDQLKATIPDIVNHLASAVRNIYQQGGRTFWVHNTGPFGCLPVNMFYMGTP 248

Query: 242 VPGDLDKNGCVKYQNDAAAEFNTQLKERLLQLRANLSDVSLVYVDVFAAKTKLISNAKEE 301
            PG LDK+GCVK QN+ A EFN +LKE ++ LR  L+  ++ YVDV+ AK +++SN K+ 
Sbjct: 249 APGYLDKSGCVKAQNEMAMEFNRKLKETVINLRKELTQAAITYVDVYTAKYEMMSNPKKL 308

Query: 302 GFRETGAICCGYHEGYNHVWCGNRAVINGSDVYAGSCPDPSKFISWDGVHYTEAANRWIA 361
           GF     +CCGYHE Y+H+WCGN+  +N +++Y GSCP+P   +SWDGVHYTEAAN+ +A
Sbjct: 309 GFANPLKVCCGYHEKYDHIWCGNKGKVNNTEIYGGSCPNPVMAVSWDGVHYTEAANKHVA 368

Query: 362 DRILHGYFSDPQVPITHACRR 377
           DR L+G  +DP VPIT AC R
Sbjct: 369 DRTLNGLLTDPPVPITRACYR 388

BLAST of MC08g_new0200 vs. ExPASy Swiss-Prot
Match: Q9LIN2 (GDSL esterase/lipase At3g26430 OS=Arabidopsis thaliana OX=3702 GN=At3g26430 PE=1 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 3.4e-94
Identity = 175/376 (46.54%), Postives = 245/376 (65.16%), Query Frame = 0

Query: 9   VAWILLSAAWI-SMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFFHKAAGR 68
           V  +LL++  I   A S +C+FPAI+NFGDSNSDTGG+SA+F     P G+TFFH  +GR
Sbjct: 8   VKCVLLASCLIHPRACSPSCNFPAIFNFGDSNSDTGGLSASFGQAPYPNGQTFFHSPSGR 67

Query: 69  GCDGRHIIDFIANHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFLNGASPFS 128
             DGR IIDFIA  L LPYL+A+L+SIG+NF HGANFAT GST+R  N ++  +G SP S
Sbjct: 68  FSDGRLIIDFIAEELGLPYLNAFLDSIGSNFSHGANFATAGSTVRPPNATIAQSGVSPIS 127

Query: 129 LDIQVVQFRQFKNRTIDLYREATAEKSIRSTLPVPEDFSKALFTMDIGQNDLVAGFR-KM 188
           LD+Q+VQF  F  R+  +       K +   LP  E FS+AL+T DIGQNDL AG +  M
Sbjct: 128 LDVQLVQFSDFITRSQLIRNRGGVFKKL---LPKKEYFSQALYTFDIGQNDLTAGLKLNM 187

Query: 189 TNEQLRSVIPDIIGEFATAVEDLYKEGARAFWVHNSGPIGCIPVAI-RSSVP-GDLDKNG 248
           T++Q+++ IPD+  + +  +  +Y +G R FW+HN+ P+GC+P  + R  VP   +D +G
Sbjct: 188 TSDQIKAYIPDVHDQLSNVIRKVYSKGGRRFWIHNTAPLGCLPYVLDRFPVPASQIDNHG 247

Query: 249 CVKYQNDAAAEFNTQLKERLLQLRANLSDVSLVYVDVFAAKTKLISNAKEEGFRETGAIC 308
           C   +N+ A  +N++LK R+++LR  LS+ +  YVD+++ K  LI+ AK+ GFR     C
Sbjct: 248 CAIPRNEIARYYNSELKRRVIELRKELSEAAFTYVDIYSIKLTLITQAKKLGFRYPLVAC 307

Query: 309 CGYHEGYNH---VWCGNRAVINGSD-VYAGSCPDPSKFISWDGVHYTEAANRWIADRILH 368
           CG+   YN    + CG + +I G + V A SC D S  +SWDG+H+TE  N WI  +I  
Sbjct: 308 CGHGGKYNFNKLIKCGAKVMIKGKEIVLAKSCNDVSFRVSWDGIHFTETTNSWIFQQIND 367

Query: 369 GYFSDPQVPITHACRR 377
           G FSDP +P+  AC R
Sbjct: 368 GAFSDPPLPVKSACTR 380

BLAST of MC08g_new0200 vs. ExPASy Swiss-Prot
Match: B4FZ87 (GDSL esterase/lipase ACHE OS=Zea mays OX=4577 GN=ACHE PE=1 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 6.4e-93
Identity = 176/356 (49.44%), Postives = 228/356 (64.04%), Query Frame = 0

Query: 27  CDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFFHKAAGRGCDGRHIIDFIANHLELPY 86
           C FPA++NFGDSNSDTGG+S+ F     P G TFF   AGR CDGR +IDFIA  L L +
Sbjct: 38  CHFPAVFNFGDSNSDTGGLSSLFGAAPPPNGRTFFGMPAGRYCDGRLVIDFIAESLGLTH 97

Query: 87  LSAYLNSIGANFRHGANFATGGSTIRRQNESVFLNGASPFSLDIQVVQFRQFKNRTIDLY 146
           LSAYLNSIG+NF  GANFAT GS+IRRQN S+FL+G SP SLD+Q  +F QF NR+  +Y
Sbjct: 98  LSAYLNSIGSNFTQGANFATAGSSIRRQNTSLFLSGFSPISLDVQFWEFEQFINRSQLVY 157

Query: 147 REATAEKSIRSTLPVPEDFSKALFTMDIGQNDLVAG-FRKMTNEQLRSVIPDIIGEFATA 206
                    R  LP  E FS+AL+T DIGQND+ +  F   T E++ ++IPD++    + 
Sbjct: 158 NNKGG--IYREILPRAEYFSQALYTFDIGQNDITSSYFVNNTTEEVEAIIPDLMERLTSI 217

Query: 207 VEDLYKEGARAFWVHNSGPIGCIPVAI--RSSVPGDLDKNGCVKYQNDAAAEFNTQLKER 266
           ++ +Y  G R FW+HN+GP+GC+P A+  R  +    D  GC    N  A  FN +LKE 
Sbjct: 218 IQSVYSRGGRYFWIHNTGPLGCLPYALLHRPDLAIPADGTGCSVTYNKVAQLFNLRLKET 277

Query: 267 LLQLRANLSDVSLVYVDVFAAKTKLISNAKEEGFRETGAICCGYHEG-YN---HVWCGNR 326
           +  LR    D +  YVDV+ AK KLIS A + GF +    CCGY  G YN    V CG +
Sbjct: 278 VASLRKTHPDAAFTYVDVYTAKYKLISQANKLGFDDPLLTCCGYGGGRYNLDLSVGCGGK 337

Query: 327 AVINGSDVYAG-SCPDPSKFISWDGVHYTEAANRWIADRILHGYFSDPQVPITHAC 375
             +NG+ V  G SC +PSK +SWDGVH+TEAAN+++ D+I+ G  SDP V +  AC
Sbjct: 338 KQVNGTSVVVGKSCENPSKRVSWDGVHFTEAANKFVFDQIVAGALSDPPVALRQAC 391

BLAST of MC08g_new0200 vs. ExPASy Swiss-Prot
Match: Q9FXE5 (Alpha-L-fucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=FXG1 PE=2 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 9.3e-92
Identity = 168/357 (47.06%), Postives = 235/357 (65.83%), Query Frame = 0

Query: 27  CDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFFHKAAGRGCDGRHIIDFIANHLELPY 86
           C FPAI+NFGDSNSDTGG+SAAF     P G +FF   AGR CDGR +IDFIA  L LPY
Sbjct: 26  CHFPAIFNFGDSNSDTGGLSAAFGQAGPPHGSSFFGSPAGRYCDGRLVIDFIAESLGLPY 85

Query: 87  LSAYLNSIGANFRHGANFATGGSTIRRQNESVFLNGASPFSLDIQVVQFRQFKNRTIDLY 146
           LSA+L+S+G+NF HGANFAT GS IR  N ++  +G SPFSLD+Q VQF  F NR+  + 
Sbjct: 86  LSAFLDSVGSNFSHGANFATAGSPIRALNSTLRQSGFSPFSLDVQFVQFYNFHNRSQTVR 145

Query: 147 REATAEKSIRSTLPVPEDFSKALFTMDIGQNDLVAG-FRKMTNEQLRSVIPDIIGEFATA 206
                 K++   LP  + FSKAL+T DIGQNDL AG F   T EQ+ + +P+II +F  A
Sbjct: 146 SRGGVYKTM---LPESDSFSKALYTFDIGQNDLTAGYFANKTVEQVETEVPEIISQFMNA 205

Query: 207 VEDLYKEGARAFWVHNSGPIGCIPVAIR--SSVPGDLDKNGCVKYQNDAAAEFNTQLKER 266
           ++++Y +G R FW+HN+GPIGC+   I    +   D D +GCV   N  A +FN  LK+ 
Sbjct: 206 IKNIYGQGGRYFWIHNTGPIGCLAYVIERFPNKASDFDSHGCVSPLNHLAQQFNHALKQA 265

Query: 267 LLQLRANLSDVSLVYVDVFAAKTKLISNAKEEGFRETGAICCGYHEGYNH---VWCGNRA 326
           +++LR++LS+ ++ YVDV++ K +L  +A+  GF+ +   CCG+   YN+   + CG + 
Sbjct: 266 VIELRSSLSEAAITYVDVYSLKHELFVHAQGHGFKGSLVSCCGHGGKYNYNKGIGCGMKK 325

Query: 327 VINGSDVYAGS-CPDPSKFISWDGVHYTEAANRWIADRILHGYFSDPQVPITHACRR 377
           ++ G +VY G  C +P K + WDGVH+T+AAN++I D+I  G        ++ AC+R
Sbjct: 326 IVKGKEVYIGKPCDEPDKAVVWDGVHFTQAANKFIFDKIAPG--------LSKACKR 371

BLAST of MC08g_new0200 vs. ExPASy Swiss-Prot
Match: Q9LII9 (GDSL esterase/lipase At3g27950 OS=Arabidopsis thaliana OX=3702 GN=At3g27950 PE=2 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 3.5e-91
Identity = 182/374 (48.66%), Postives = 233/374 (62.30%), Query Frame = 0

Query: 2   KIQMAFTVAWILLSAAWISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFF 61
           KI +A  V  +LL       A S +C+FPA++NFGDSNSDTG ISAA      P G  FF
Sbjct: 5   KITLAIIV--LLLGFTEKLSALSSSCNFPAVFNFGDSNSDTGAISAAIGEVPPPNGVAFF 64

Query: 62  HKAAGRGCDGRHIIDFIANHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFLN 121
            ++AGR  DGR IIDFI  +L LPYL+ YL+S+GAN+RHGANFATGGS IR       L 
Sbjct: 65  GRSAGRHSDGRLIIDFITENLTLPYLTPYLDSVGANYRHGANFATGGSCIRPT-----LA 124

Query: 122 GASPFSLDIQVVQFRQFKNRTIDLYREATAEKSIRSTLPVPEDFSKALFTMDIGQNDLVA 181
             SPF L  QV QF  FK RT+ LY +   +    + L     FSKAL+T+DIGQNDL  
Sbjct: 125 CFSPFHLGTQVSQFIHFKTRTLSLYNQTNGK---FNRLSHTNYFSKALYTLDIGQNDLAI 184

Query: 182 GFRKMTNEQLRSVIPDIIGEFATAVEDLYKEGARAFWVHNSGPIGCIPVAIRSSVPGDLD 241
           GF+ MT EQL++ IP II  F  A++ LYKEGAR F +HN+GP GC+P  +++      D
Sbjct: 185 GFQNMTEEQLKATIPLIIENFTIALKLLYKEGARFFSIHNTGPTGCLPYLLKAFPAIPRD 244

Query: 242 KNGCVKYQNDAAAEFNTQLKERLLQLRANLSDVSLVYVDVFAAKTKLISNAKEEGFRETG 301
             GC+K  N+ A EFN QLK ++ QL+  L      YVDV++AK  LI+ AK  GF +  
Sbjct: 245 PYGCLKPLNNVAIEFNKQLKNKITQLKKELPSSFFTYVDVYSAKYNLITKAKALGFIDPF 304

Query: 302 AICCGYHEGYNHVWCGNRAVINGSDVYAGSCPDPSKFISWDGVHYTEAANRWIADRILHG 361
             CC    G   + CG    +NG+++Y+ SC +   FISWDG+HYTE AN  +A+RIL G
Sbjct: 305 DYCCVGAIG-RGMGCGKTIFLNGTELYSSSCQNRKNFISWDGIHYTETANMLVANRILDG 364

Query: 362 YFSDPQVPITHACR 376
             SDP +P   AC+
Sbjct: 365 SISDPPLPTQKACK 367

BLAST of MC08g_new0200 vs. NCBI nr
Match: XP_022152138.1 (GDSL esterase/lipase At5g14450-like [Momordica charantia])

HSP 1 Score: 784 bits (2025), Expect = 1.50e-286
Identity = 380/380 (100.00%), Postives = 380/380 (100.00%), Query Frame = 0

Query: 1   MKIQMAFTVAWILLSAAWISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETF 60
           MKIQMAFTVAWILLSAAWISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETF
Sbjct: 1   MKIQMAFTVAWILLSAAWISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETF 60

Query: 61  FHKAAGRGCDGRHIIDFIANHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFL 120
           FHKAAGRGCDGRHIIDFIANHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFL
Sbjct: 61  FHKAAGRGCDGRHIIDFIANHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFL 120

Query: 121 NGASPFSLDIQVVQFRQFKNRTIDLYREATAEKSIRSTLPVPEDFSKALFTMDIGQNDLV 180
           NGASPFSLDIQVVQFRQFKNRTIDLYREATAEKSIRSTLPVPEDFSKALFTMDIGQNDLV
Sbjct: 121 NGASPFSLDIQVVQFRQFKNRTIDLYREATAEKSIRSTLPVPEDFSKALFTMDIGQNDLV 180

Query: 181 AGFRKMTNEQLRSVIPDIIGEFATAVEDLYKEGARAFWVHNSGPIGCIPVAIRSSVPGDL 240
           AGFRKMTNEQLRSVIPDIIGEFATAVEDLYKEGARAFWVHNSGPIGCIPVAIRSSVPGDL
Sbjct: 181 AGFRKMTNEQLRSVIPDIIGEFATAVEDLYKEGARAFWVHNSGPIGCIPVAIRSSVPGDL 240

Query: 241 DKNGCVKYQNDAAAEFNTQLKERLLQLRANLSDVSLVYVDVFAAKTKLISNAKEEGFRET 300
           DKNGCVKYQNDAAAEFNTQLKERLLQLRANLSDVSLVYVDVFAAKTKLISNAKEEGFRET
Sbjct: 241 DKNGCVKYQNDAAAEFNTQLKERLLQLRANLSDVSLVYVDVFAAKTKLISNAKEEGFRET 300

Query: 301 GAICCGYHEGYNHVWCGNRAVINGSDVYAGSCPDPSKFISWDGVHYTEAANRWIADRILH 360
           GAICCGYHEGYNHVWCGNRAVINGSDVYAGSCPDPSKFISWDGVHYTEAANRWIADRILH
Sbjct: 301 GAICCGYHEGYNHVWCGNRAVINGSDVYAGSCPDPSKFISWDGVHYTEAANRWIADRILH 360

Query: 361 GYFSDPQVPITHACRRFSEG 380
           GYFSDPQVPITHACRRFSEG
Sbjct: 361 GYFSDPQVPITHACRRFSEG 380

BLAST of MC08g_new0200 vs. NCBI nr
Match: XP_038905601.1 (GDSL esterase/lipase At5g14450-like isoform X1 [Benincasa hispida])

HSP 1 Score: 632 bits (1629), Expect = 2.88e-226
Identity = 307/378 (81.22%), Postives = 333/378 (88.10%), Query Frame = 0

Query: 1   MKIQMAFTVAWILLSAAWISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETF 60
           MKIQMA TVA IL     ISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETF
Sbjct: 5   MKIQMALTVASIL-----ISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETF 64

Query: 61  FHKAAGRGCDGRHIIDFIANHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFL 120
           FHK AGRGCDGR IIDFIA HLELPYLSAYLNSIG NFRHGANFATGGSTIRRQNESVF 
Sbjct: 65  FHKTAGRGCDGRLIIDFIAKHLELPYLSAYLNSIGTNFRHGANFATGGSTIRRQNESVFE 124

Query: 121 NGASPFSLDIQVVQFRQFKNRTIDLYREATAEKSIRSTLPVPEDFSKALFTMDIGQNDLV 180
           NG SPFSLDIQ+VQFRQFKNRTID Y EA  + SI++TLP+PE+FSKA+FT+DIGQNDL 
Sbjct: 125 NGVSPFSLDIQIVQFRQFKNRTIDRYLEAV-DDSIKNTLPIPEEFSKAIFTIDIGQNDLS 184

Query: 181 AGFRKMTNEQLRSVIPDIIGEFATAVEDLYKEGARAFWVHNSGPIGCIPVAIRS---SVP 240
           AGFRKMTN Q  + IP+II EF+ A+EDLYKEGARAFWVHN+GPIGCIPVAIRS    +P
Sbjct: 185 AGFRKMTNAQFLTTIPNIIDEFSRAIEDLYKEGARAFWVHNTGPIGCIPVAIRSIPNPIP 244

Query: 241 GDLDKNGCVKYQNDAAAEFNTQLKERLLQLRANLSDVSLVYVDVFAAKTKLISNAKEEGF 300
           GD D+NGCV+YQNDAA EFN QLKER+++LR  LSD SLVYVDVFAAK KLI+NAKEEGF
Sbjct: 245 GDFDQNGCVRYQNDAALEFNKQLKERIIKLRTILSDASLVYVDVFAAKLKLIANAKEEGF 304

Query: 301 RETGAICCGYHEGYNHVWCGNRAVINGSDVYAGSCPDPSKFISWDGVHYTEAANRWIADR 360
            E G ICCGYHEG NHVWCGNR +INGS+VY GSC DPSKFISWDGVHYTEAANRWIA++
Sbjct: 305 MEKGEICCGYHEGNNHVWCGNRKIINGSEVYGGSCDDPSKFISWDGVHYTEAANRWIANQ 364

Query: 361 ILHGYFSDPQVPITHACR 375
           I+ G FSDPQ+PITHACR
Sbjct: 365 IVKGSFSDPQIPITHACR 376

BLAST of MC08g_new0200 vs. NCBI nr
Match: XP_004141199.2 (GDSL esterase/lipase At5g14450 [Cucumis sativus] >KGN59717.1 hypothetical protein Csa_001756 [Cucumis sativus])

HSP 1 Score: 630 bits (1626), Expect = 9.21e-226
Identity = 314/378 (83.07%), Postives = 336/378 (88.89%), Query Frame = 0

Query: 1   MKIQMAFTVAWILLSAAWISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETF 60
           MK++M F+VA I+L    ISMADSRAC+FPAIYNFGDSNSDTGGISAAFYPTILPCG+TF
Sbjct: 7   MKMKMVFSVASIIL----ISMADSRACNFPAIYNFGDSNSDTGGISAAFYPTILPCGQTF 66

Query: 61  FHKAAGRGCDGRHIIDFIANHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFL 120
           FHK AGRGCDGR IIDFIA  LELPYLSAYLNSIG NFRHGANFATGGSTIRRQNESVF 
Sbjct: 67  FHKTAGRGCDGRLIIDFIAKQLELPYLSAYLNSIGTNFRHGANFATGGSTIRRQNESVFE 126

Query: 121 NGASPFSLDIQVVQFRQFKNRTIDLYREATAEKSIRSTLPVPEDFSKALFTMDIGQNDLV 180
           NG SPFSLDIQVVQFRQFKNRTID Y EA  + SIRSTLPVPE+FSKALFT+DIGQNDL 
Sbjct: 127 NGISPFSLDIQVVQFRQFKNRTIDRYVEAI-DDSIRSTLPVPEEFSKALFTIDIGQNDLS 186

Query: 181 AGFRKMTNEQLRSVIPDIIGEFATAVEDLYKEGARAFWVHNSGPIGCIPVAIRS-SVP-- 240
           AGFRKMTN+Q R  IPDII EFATAVEDLYKEGARAFWVHN+GPIGCIPVAIRS S P  
Sbjct: 187 AGFRKMTNDQFRKAIPDIINEFATAVEDLYKEGARAFWVHNTGPIGCIPVAIRSVSNPKE 246

Query: 241 GDLDKNGCVKYQNDAAAEFNTQLKERLLQLRANLSDVSLVYVDVFAAKTKLISNAKEEGF 300
           GDLD+NGCVK QNDAA EFN QLKER+++LRANL D SLVYVDV+AAK KLI+NAKEEGF
Sbjct: 247 GDLDRNGCVKEQNDAALEFNRQLKERVVKLRANLLDASLVYVDVYAAKIKLIANAKEEGF 306

Query: 301 RETGAICCGYHEGYNHVWCGNRAVINGSDVYAGSCPDPSKFISWDGVHYTEAANRWIADR 360
            E GAICCGYHEG NHVWCGNR  INGS+VYAGSC DPSKFISWDGVHYTEAAN+WIA++
Sbjct: 307 MEKGAICCGYHEGLNHVWCGNRKTINGSEVYAGSCEDPSKFISWDGVHYTEAANQWIANQ 366

Query: 361 ILHGYFSDPQVPITHACR 375
           I+ G FSDPQVPI HACR
Sbjct: 367 IVKGSFSDPQVPIMHACR 379

BLAST of MC08g_new0200 vs. NCBI nr
Match: XP_038905602.1 (GDSL esterase/lipase At5g14450-like isoform X2 [Benincasa hispida])

HSP 1 Score: 630 bits (1624), Expect = 1.60e-225
Identity = 306/378 (80.95%), Postives = 332/378 (87.83%), Query Frame = 0

Query: 1   MKIQMAFTVAWILLSAAWISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETF 60
           MKIQMA TVA IL     ISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETF
Sbjct: 5   MKIQMALTVASIL-----ISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETF 64

Query: 61  FHKAAGRGCDGRHIIDFIANHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFL 120
           FHK AGRGCDGR IIDFIA HLELPYLSAYLNSIG NFRHGANFATGGSTIRRQNESVF 
Sbjct: 65  FHKTAGRGCDGRLIIDFIAKHLELPYLSAYLNSIGTNFRHGANFATGGSTIRRQNESVFE 124

Query: 121 NGASPFSLDIQVVQFRQFKNRTIDLYREATAEKSIRSTLPVPEDFSKALFTMDIGQNDLV 180
           NG SPFSLDIQ+VQFRQFKNRTID Y E   + SI++TLP+PE+FSKA+FT+DIGQNDL 
Sbjct: 125 NGVSPFSLDIQIVQFRQFKNRTIDRYLEV--DDSIKNTLPIPEEFSKAIFTIDIGQNDLS 184

Query: 181 AGFRKMTNEQLRSVIPDIIGEFATAVEDLYKEGARAFWVHNSGPIGCIPVAIRS---SVP 240
           AGFRKMTN Q  + IP+II EF+ A+EDLYKEGARAFWVHN+GPIGCIPVAIRS    +P
Sbjct: 185 AGFRKMTNAQFLTTIPNIIDEFSRAIEDLYKEGARAFWVHNTGPIGCIPVAIRSIPNPIP 244

Query: 241 GDLDKNGCVKYQNDAAAEFNTQLKERLLQLRANLSDVSLVYVDVFAAKTKLISNAKEEGF 300
           GD D+NGCV+YQNDAA EFN QLKER+++LR  LSD SLVYVDVFAAK KLI+NAKEEGF
Sbjct: 245 GDFDQNGCVRYQNDAALEFNKQLKERIIKLRTILSDASLVYVDVFAAKLKLIANAKEEGF 304

Query: 301 RETGAICCGYHEGYNHVWCGNRAVINGSDVYAGSCPDPSKFISWDGVHYTEAANRWIADR 360
            E G ICCGYHEG NHVWCGNR +INGS+VY GSC DPSKFISWDGVHYTEAANRWIA++
Sbjct: 305 MEKGEICCGYHEGNNHVWCGNRKIINGSEVYGGSCDDPSKFISWDGVHYTEAANRWIANQ 364

Query: 361 ILHGYFSDPQVPITHACR 375
           I+ G FSDPQ+PITHACR
Sbjct: 365 IVKGSFSDPQIPITHACR 375

BLAST of MC08g_new0200 vs. NCBI nr
Match: XP_022982674.1 (GDSL esterase/lipase At5g14450-like [Cucurbita maxima])

HSP 1 Score: 628 bits (1620), Expect = 5.83e-225
Identity = 309/376 (82.18%), Postives = 333/376 (88.56%), Query Frame = 0

Query: 1   MKIQMAF-TVAWILLSAAWISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGET 60
           MKI MAF TVA IL     ISMADSRAC FPAIYNFGDSNSDTGGISAAFYPTILPCGET
Sbjct: 3   MKIPMAFFTVASIL-----ISMADSRACGFPAIYNFGDSNSDTGGISAAFYPTILPCGET 62

Query: 61  FFHKAAGRGCDGRHIIDFIANHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVF 120
           FFHK AGRGCDGR IIDF+A HLELPYLSAYLNSIGANFRHGANFATGGSTIR QNESVF
Sbjct: 63  FFHKTAGRGCDGRLIIDFLAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRNQNESVF 122

Query: 121 LNGASPFSLDIQVVQFRQFKNRTIDLYREATAEKSIRSTLPVPEDFSKALFTMDIGQNDL 180
            NG SPFSLDIQVVQFRQFKNRTID Y E T +  IRSTLPVPE+FS ALFT+DIGQNDL
Sbjct: 123 ENGVSPFSLDIQVVQFRQFKNRTIDRYHEETND-FIRSTLPVPEEFSNALFTIDIGQNDL 182

Query: 181 VAGFRKMTNEQLRSVIPDIIGEFATAVEDLYKEGARAFWVHNSGPIGCIPVAIRSSVPGD 240
            AGFRKMTN Q +  IP+IIGEF  A+E+LY+EGARAFWVHN+GPIGCIPVAIR++V GD
Sbjct: 183 SAGFRKMTNYQFQMAIPNIIGEFDAAIEELYREGARAFWVHNTGPIGCIPVAIRTTVHGD 242

Query: 241 LDKNGCVKYQNDAAAEFNTQLKERLLQLRANLSDVSLVYVDVFAAKTKLISNAKEEGFRE 300
           LD+NGCVKYQNDAA EFN QLKER++QLRA LSD SLVYVDVFAAK +LI+NAKEEGF E
Sbjct: 243 LDQNGCVKYQNDAALEFNRQLKERVIQLRAKLSDASLVYVDVFAAKLQLIANAKEEGFME 302

Query: 301 TGAICCGYHEGYNHVWCGNRAVINGSDVYAGSCPDPSKFISWDGVHYTEAANRWIADRIL 360
            GAICCGYHEG +HVWCGN+ +INGS VYAGSC DPS+FISWDGVHYTEAANRWIA++I+
Sbjct: 303 MGAICCGYHEGNDHVWCGNQKIINGSQVYAGSCDDPSRFISWDGVHYTEAANRWIANQII 362

Query: 361 HGYFSDPQVPITHACR 375
            G FSDPQVPITHACR
Sbjct: 363 KGSFSDPQVPITHACR 372

BLAST of MC08g_new0200 vs. ExPASy TrEMBL
Match: A0A6J1DE21 (GDSL esterase/lipase At5g14450-like OS=Momordica charantia OX=3673 GN=LOC111019926 PE=3 SV=1)

HSP 1 Score: 784 bits (2025), Expect = 7.29e-287
Identity = 380/380 (100.00%), Postives = 380/380 (100.00%), Query Frame = 0

Query: 1   MKIQMAFTVAWILLSAAWISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETF 60
           MKIQMAFTVAWILLSAAWISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETF
Sbjct: 1   MKIQMAFTVAWILLSAAWISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETF 60

Query: 61  FHKAAGRGCDGRHIIDFIANHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFL 120
           FHKAAGRGCDGRHIIDFIANHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFL
Sbjct: 61  FHKAAGRGCDGRHIIDFIANHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFL 120

Query: 121 NGASPFSLDIQVVQFRQFKNRTIDLYREATAEKSIRSTLPVPEDFSKALFTMDIGQNDLV 180
           NGASPFSLDIQVVQFRQFKNRTIDLYREATAEKSIRSTLPVPEDFSKALFTMDIGQNDLV
Sbjct: 121 NGASPFSLDIQVVQFRQFKNRTIDLYREATAEKSIRSTLPVPEDFSKALFTMDIGQNDLV 180

Query: 181 AGFRKMTNEQLRSVIPDIIGEFATAVEDLYKEGARAFWVHNSGPIGCIPVAIRSSVPGDL 240
           AGFRKMTNEQLRSVIPDIIGEFATAVEDLYKEGARAFWVHNSGPIGCIPVAIRSSVPGDL
Sbjct: 181 AGFRKMTNEQLRSVIPDIIGEFATAVEDLYKEGARAFWVHNSGPIGCIPVAIRSSVPGDL 240

Query: 241 DKNGCVKYQNDAAAEFNTQLKERLLQLRANLSDVSLVYVDVFAAKTKLISNAKEEGFRET 300
           DKNGCVKYQNDAAAEFNTQLKERLLQLRANLSDVSLVYVDVFAAKTKLISNAKEEGFRET
Sbjct: 241 DKNGCVKYQNDAAAEFNTQLKERLLQLRANLSDVSLVYVDVFAAKTKLISNAKEEGFRET 300

Query: 301 GAICCGYHEGYNHVWCGNRAVINGSDVYAGSCPDPSKFISWDGVHYTEAANRWIADRILH 360
           GAICCGYHEGYNHVWCGNRAVINGSDVYAGSCPDPSKFISWDGVHYTEAANRWIADRILH
Sbjct: 301 GAICCGYHEGYNHVWCGNRAVINGSDVYAGSCPDPSKFISWDGVHYTEAANRWIADRILH 360

Query: 361 GYFSDPQVPITHACRRFSEG 380
           GYFSDPQVPITHACRRFSEG
Sbjct: 361 GYFSDPQVPITHACRRFSEG 380

BLAST of MC08g_new0200 vs. ExPASy TrEMBL
Match: A0A0A0LG79 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G840430 PE=3 SV=1)

HSP 1 Score: 630 bits (1626), Expect = 4.46e-226
Identity = 314/378 (83.07%), Postives = 336/378 (88.89%), Query Frame = 0

Query: 1   MKIQMAFTVAWILLSAAWISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETF 60
           MK++M F+VA I+L    ISMADSRAC+FPAIYNFGDSNSDTGGISAAFYPTILPCG+TF
Sbjct: 7   MKMKMVFSVASIIL----ISMADSRACNFPAIYNFGDSNSDTGGISAAFYPTILPCGQTF 66

Query: 61  FHKAAGRGCDGRHIIDFIANHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFL 120
           FHK AGRGCDGR IIDFIA  LELPYLSAYLNSIG NFRHGANFATGGSTIRRQNESVF 
Sbjct: 67  FHKTAGRGCDGRLIIDFIAKQLELPYLSAYLNSIGTNFRHGANFATGGSTIRRQNESVFE 126

Query: 121 NGASPFSLDIQVVQFRQFKNRTIDLYREATAEKSIRSTLPVPEDFSKALFTMDIGQNDLV 180
           NG SPFSLDIQVVQFRQFKNRTID Y EA  + SIRSTLPVPE+FSKALFT+DIGQNDL 
Sbjct: 127 NGISPFSLDIQVVQFRQFKNRTIDRYVEAI-DDSIRSTLPVPEEFSKALFTIDIGQNDLS 186

Query: 181 AGFRKMTNEQLRSVIPDIIGEFATAVEDLYKEGARAFWVHNSGPIGCIPVAIRS-SVP-- 240
           AGFRKMTN+Q R  IPDII EFATAVEDLYKEGARAFWVHN+GPIGCIPVAIRS S P  
Sbjct: 187 AGFRKMTNDQFRKAIPDIINEFATAVEDLYKEGARAFWVHNTGPIGCIPVAIRSVSNPKE 246

Query: 241 GDLDKNGCVKYQNDAAAEFNTQLKERLLQLRANLSDVSLVYVDVFAAKTKLISNAKEEGF 300
           GDLD+NGCVK QNDAA EFN QLKER+++LRANL D SLVYVDV+AAK KLI+NAKEEGF
Sbjct: 247 GDLDRNGCVKEQNDAALEFNRQLKERVVKLRANLLDASLVYVDVYAAKIKLIANAKEEGF 306

Query: 301 RETGAICCGYHEGYNHVWCGNRAVINGSDVYAGSCPDPSKFISWDGVHYTEAANRWIADR 360
            E GAICCGYHEG NHVWCGNR  INGS+VYAGSC DPSKFISWDGVHYTEAAN+WIA++
Sbjct: 307 MEKGAICCGYHEGLNHVWCGNRKTINGSEVYAGSCEDPSKFISWDGVHYTEAANQWIANQ 366

Query: 361 ILHGYFSDPQVPITHACR 375
           I+ G FSDPQVPI HACR
Sbjct: 367 IVKGSFSDPQVPIMHACR 379

BLAST of MC08g_new0200 vs. ExPASy TrEMBL
Match: A0A6J1J589 (GDSL esterase/lipase At5g14450-like OS=Cucurbita maxima OX=3661 GN=LOC111481479 PE=3 SV=1)

HSP 1 Score: 628 bits (1620), Expect = 2.82e-225
Identity = 309/376 (82.18%), Postives = 333/376 (88.56%), Query Frame = 0

Query: 1   MKIQMAF-TVAWILLSAAWISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGET 60
           MKI MAF TVA IL     ISMADSRAC FPAIYNFGDSNSDTGGISAAFYPTILPCGET
Sbjct: 3   MKIPMAFFTVASIL-----ISMADSRACGFPAIYNFGDSNSDTGGISAAFYPTILPCGET 62

Query: 61  FFHKAAGRGCDGRHIIDFIANHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVF 120
           FFHK AGRGCDGR IIDF+A HLELPYLSAYLNSIGANFRHGANFATGGSTIR QNESVF
Sbjct: 63  FFHKTAGRGCDGRLIIDFLAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRNQNESVF 122

Query: 121 LNGASPFSLDIQVVQFRQFKNRTIDLYREATAEKSIRSTLPVPEDFSKALFTMDIGQNDL 180
            NG SPFSLDIQVVQFRQFKNRTID Y E T +  IRSTLPVPE+FS ALFT+DIGQNDL
Sbjct: 123 ENGVSPFSLDIQVVQFRQFKNRTIDRYHEETND-FIRSTLPVPEEFSNALFTIDIGQNDL 182

Query: 181 VAGFRKMTNEQLRSVIPDIIGEFATAVEDLYKEGARAFWVHNSGPIGCIPVAIRSSVPGD 240
            AGFRKMTN Q +  IP+IIGEF  A+E+LY+EGARAFWVHN+GPIGCIPVAIR++V GD
Sbjct: 183 SAGFRKMTNYQFQMAIPNIIGEFDAAIEELYREGARAFWVHNTGPIGCIPVAIRTTVHGD 242

Query: 241 LDKNGCVKYQNDAAAEFNTQLKERLLQLRANLSDVSLVYVDVFAAKTKLISNAKEEGFRE 300
           LD+NGCVKYQNDAA EFN QLKER++QLRA LSD SLVYVDVFAAK +LI+NAKEEGF E
Sbjct: 243 LDQNGCVKYQNDAALEFNRQLKERVIQLRAKLSDASLVYVDVFAAKLQLIANAKEEGFME 302

Query: 301 TGAICCGYHEGYNHVWCGNRAVINGSDVYAGSCPDPSKFISWDGVHYTEAANRWIADRIL 360
            GAICCGYHEG +HVWCGN+ +INGS VYAGSC DPS+FISWDGVHYTEAANRWIA++I+
Sbjct: 303 MGAICCGYHEGNDHVWCGNQKIINGSQVYAGSCDDPSRFISWDGVHYTEAANRWIANQII 362

Query: 361 HGYFSDPQVPITHACR 375
            G FSDPQVPITHACR
Sbjct: 363 KGSFSDPQVPITHACR 372

BLAST of MC08g_new0200 vs. ExPASy TrEMBL
Match: A0A1S3CN47 (GDSL esterase/lipase At5g14450-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502686 PE=3 SV=1)

HSP 1 Score: 624 bits (1610), Expect = 1.31e-223
Identity = 306/381 (80.31%), Postives = 334/381 (87.66%), Query Frame = 0

Query: 1   MKIQMAFTVAWILLSAAWISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETF 60
           MKIQ+AFTVA I+L    ISM DSRAC+FPAIYNFGDSNSDTGGISAAFYPTILPCG+TF
Sbjct: 5   MKIQLAFTVASIIL----ISMVDSRACNFPAIYNFGDSNSDTGGISAAFYPTILPCGQTF 64

Query: 61  FHKAAGRGCDGRHIIDFIANHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFL 120
           FHK AGRGCDGR IIDF A HLELPYLSAYLNSIG NFRHGANFATGGSTIRRQNESVF 
Sbjct: 65  FHKTAGRGCDGRLIIDFFAKHLELPYLSAYLNSIGTNFRHGANFATGGSTIRRQNESVFE 124

Query: 121 NGASPFSLDIQVVQFRQFKNRTIDLYREATAEKSIRSTLPVPEDFSKALFTMDIGQNDLV 180
           NG SPF LDIQVVQFRQFKNRTID Y E   + SIRS+LPVPE+FSKALFT+DIGQNDL 
Sbjct: 125 NGVSPFPLDIQVVQFRQFKNRTIDRYVEV--DGSIRSSLPVPEEFSKALFTIDIGQNDLS 184

Query: 181 AGFRKMTNEQLRSVIPDIIGEFATAVEDLYKEGARAFWVHNSGPIGCIPVAIRSSV---P 240
           AGFRKMTN+Q +  IPDII EFATAVEDLYKEG RAFWVHN+GPIGCIPVAIRS     P
Sbjct: 185 AGFRKMTNDQFQKAIPDIINEFATAVEDLYKEGGRAFWVHNTGPIGCIPVAIRSISKPNP 244

Query: 241 GDLDKNGCVKYQNDAAAEFNTQLKERLLQLRANLSDVSLVYVDVFAAKTKLISNAKEEGF 300
           GDLD+NGCVKYQN+AA EFN QLKER+++LRA LS+  +VYVDV+AAK KLI+NAKEEGF
Sbjct: 245 GDLDQNGCVKYQNNAALEFNRQLKERVVKLRAKLSNAFIVYVDVYAAKIKLIANAKEEGF 304

Query: 301 RETGAICCGYHEGYNHVWCGNRAVINGSDVYAGSCPDPSKFISWDGVHYTEAANRWIADR 360
            E GAICCGYHEG +HVWCGN+ +INGS+VYAGSC DPSKFISWDGVHYTEAAN+WIA++
Sbjct: 305 MEKGAICCGYHEGVDHVWCGNKKIINGSEVYAGSCKDPSKFISWDGVHYTEAANQWIANQ 364

Query: 361 ILHGYFSDPQVPITHACRRFS 378
           I+ G FSDPQVPI HAC  FS
Sbjct: 365 IVDGSFSDPQVPIIHACPSFS 379

BLAST of MC08g_new0200 vs. ExPASy TrEMBL
Match: A0A1S3CP81 (GDSL esterase/lipase At5g14450-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103502686 PE=3 SV=1)

HSP 1 Score: 624 bits (1609), Expect = 1.80e-223
Identity = 306/381 (80.31%), Postives = 334/381 (87.66%), Query Frame = 0

Query: 1   MKIQMAFTVAWILLSAAWISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETF 60
           MKIQ+AFTVA I+L    ISM DSRAC+FPAIYNFGDSNSDTGGISAAFYPTILPCG+TF
Sbjct: 5   MKIQLAFTVASIIL----ISMVDSRACNFPAIYNFGDSNSDTGGISAAFYPTILPCGQTF 64

Query: 61  FHKAAGRGCDGRHIIDFIANHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFL 120
           FHK AGRGCDGR IIDF A HLELPYLSAYLNSIG NFRHGANFATGGSTIRRQNESVF 
Sbjct: 65  FHKTAGRGCDGRLIIDFFAKHLELPYLSAYLNSIGTNFRHGANFATGGSTIRRQNESVFE 124

Query: 121 NGASPFSLDIQVVQFRQFKNRTIDLYREATAEKSIRSTLPVPEDFSKALFTMDIGQNDLV 180
           NG SPF LDIQVVQFRQFKNRTID Y E   + SIRS+LPVPE+FSKALFT+DIGQNDL 
Sbjct: 125 NGVSPFPLDIQVVQFRQFKNRTIDRYVE---DGSIRSSLPVPEEFSKALFTIDIGQNDLS 184

Query: 181 AGFRKMTNEQLRSVIPDIIGEFATAVEDLYKEGARAFWVHNSGPIGCIPVAIRSSV---P 240
           AGFRKMTN+Q +  IPDII EFATAVEDLYKEG RAFWVHN+GPIGCIPVAIRS     P
Sbjct: 185 AGFRKMTNDQFQKAIPDIINEFATAVEDLYKEGGRAFWVHNTGPIGCIPVAIRSISKPNP 244

Query: 241 GDLDKNGCVKYQNDAAAEFNTQLKERLLQLRANLSDVSLVYVDVFAAKTKLISNAKEEGF 300
           GDLD+NGCVKYQN+AA EFN QLKER+++LRA LS+  +VYVDV+AAK KLI+NAKEEGF
Sbjct: 245 GDLDQNGCVKYQNNAALEFNRQLKERVVKLRAKLSNAFIVYVDVYAAKIKLIANAKEEGF 304

Query: 301 RETGAICCGYHEGYNHVWCGNRAVINGSDVYAGSCPDPSKFISWDGVHYTEAANRWIADR 360
            E GAICCGYHEG +HVWCGN+ +INGS+VYAGSC DPSKFISWDGVHYTEAAN+WIA++
Sbjct: 305 MEKGAICCGYHEGVDHVWCGNKKIINGSEVYAGSCKDPSKFISWDGVHYTEAANQWIANQ 364

Query: 361 ILHGYFSDPQVPITHACRRFS 378
           I+ G FSDPQVPI HAC  FS
Sbjct: 365 IVDGSFSDPQVPIIHACPSFS 378

BLAST of MC08g_new0200 vs. TAIR 10
Match: AT5G14450.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 451.4 bits (1160), Expect = 7.0e-127
Identity = 214/381 (56.17%), Postives = 277/381 (72.70%), Query Frame = 0

Query: 2   KIQMAFTV-AWILLS--AAWISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGE 61
           K+ ++ TV +W+LL   A   S++    C FPAIYNFGDSNSDTGGISAAF P   P G+
Sbjct: 9   KLMVSSTVFSWLLLCLFAVTTSVSVQPTCTFPAIYNFGDSNSDTGGISAAFEPIRDPYGQ 68

Query: 62  TFFHKAAGRGCDGRHIIDFIANHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESV 121
            FFH+  GR  DGR  IDFIA  L LPYLSAYLNS+G+NFRHGANFATGGSTIRRQNE++
Sbjct: 69  GFFHRPTGRDSDGRLTIDFIAERLGLPYLSAYLNSLGSNFRHGANFATGGSTIRRQNETI 128

Query: 122 FLNGASPFSLDIQVVQFRQFKNRTIDLYREATAEKSIRSTLPVPEDFSKALFTMDIGQND 181
           F  G SPFSLD+Q+ QF QFK R+  L+ +  +    R  LP  E+F+KAL+T DIGQND
Sbjct: 129 FQYGISPFSLDMQIAQFDQFKARSALLFTQIKSRYD-REKLPRQEEFAKALYTFDIGQND 188

Query: 182 LVAGFRKMTNEQLRSVIPDIIGEFATAVEDLYKEGARAFWVHNSGPIGCIPV---AIRSS 241
           L  GFR M+ +QL++ IPDI+   A+AV ++Y++G R FWVHN+GP GC+PV    + + 
Sbjct: 189 LSVGFRTMSVDQLKATIPDIVNHLASAVRNIYQQGGRTFWVHNTGPFGCLPVNMFYMGTP 248

Query: 242 VPGDLDKNGCVKYQNDAAAEFNTQLKERLLQLRANLSDVSLVYVDVFAAKTKLISNAKEE 301
            PG LDK+GCVK QN+ A EFN +LKE ++ LR  L+  ++ YVDV+ AK +++SN K+ 
Sbjct: 249 APGYLDKSGCVKAQNEMAMEFNRKLKETVINLRKELTQAAITYVDVYTAKYEMMSNPKKL 308

Query: 302 GFRETGAICCGYHEGYNHVWCGNRAVINGSDVYAGSCPDPSKFISWDGVHYTEAANRWIA 361
           GF     +CCGYHE Y+H+WCGN+  +N +++Y GSCP+P   +SWDGVHYTEAAN+ +A
Sbjct: 309 GFANPLKVCCGYHEKYDHIWCGNKGKVNNTEIYGGSCPNPVMAVSWDGVHYTEAANKHVA 368

Query: 362 DRILHGYFSDPQVPITHACRR 377
           DR L+G  +DP VPIT AC R
Sbjct: 369 DRTLNGLLTDPPVPITRACYR 388

BLAST of MC08g_new0200 vs. TAIR 10
Match: AT3G26430.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 346.7 bits (888), Expect = 2.4e-95
Identity = 175/376 (46.54%), Postives = 245/376 (65.16%), Query Frame = 0

Query: 9   VAWILLSAAWI-SMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFFHKAAGR 68
           V  +LL++  I   A S +C+FPAI+NFGDSNSDTGG+SA+F     P G+TFFH  +GR
Sbjct: 8   VKCVLLASCLIHPRACSPSCNFPAIFNFGDSNSDTGGLSASFGQAPYPNGQTFFHSPSGR 67

Query: 69  GCDGRHIIDFIANHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFLNGASPFS 128
             DGR IIDFIA  L LPYL+A+L+SIG+NF HGANFAT GST+R  N ++  +G SP S
Sbjct: 68  FSDGRLIIDFIAEELGLPYLNAFLDSIGSNFSHGANFATAGSTVRPPNATIAQSGVSPIS 127

Query: 129 LDIQVVQFRQFKNRTIDLYREATAEKSIRSTLPVPEDFSKALFTMDIGQNDLVAGFR-KM 188
           LD+Q+VQF  F  R+  +       K +   LP  E FS+AL+T DIGQNDL AG +  M
Sbjct: 128 LDVQLVQFSDFITRSQLIRNRGGVFKKL---LPKKEYFSQALYTFDIGQNDLTAGLKLNM 187

Query: 189 TNEQLRSVIPDIIGEFATAVEDLYKEGARAFWVHNSGPIGCIPVAI-RSSVP-GDLDKNG 248
           T++Q+++ IPD+  + +  +  +Y +G R FW+HN+ P+GC+P  + R  VP   +D +G
Sbjct: 188 TSDQIKAYIPDVHDQLSNVIRKVYSKGGRRFWIHNTAPLGCLPYVLDRFPVPASQIDNHG 247

Query: 249 CVKYQNDAAAEFNTQLKERLLQLRANLSDVSLVYVDVFAAKTKLISNAKEEGFRETGAIC 308
           C   +N+ A  +N++LK R+++LR  LS+ +  YVD+++ K  LI+ AK+ GFR     C
Sbjct: 248 CAIPRNEIARYYNSELKRRVIELRKELSEAAFTYVDIYSIKLTLITQAKKLGFRYPLVAC 307

Query: 309 CGYHEGYNH---VWCGNRAVINGSD-VYAGSCPDPSKFISWDGVHYTEAANRWIADRILH 368
           CG+   YN    + CG + +I G + V A SC D S  +SWDG+H+TE  N WI  +I  
Sbjct: 308 CGHGGKYNFNKLIKCGAKVMIKGKEIVLAKSCNDVSFRVSWDGIHFTETTNSWIFQQIND 367

Query: 369 GYFSDPQVPITHACRR 377
           G FSDP +P+  AC R
Sbjct: 368 GAFSDPPLPVKSACTR 380

BLAST of MC08g_new0200 vs. TAIR 10
Match: AT1G67830.1 (alpha-fucosidase 1 )

HSP 1 Score: 338.6 bits (867), Expect = 6.6e-93
Identity = 168/357 (47.06%), Postives = 235/357 (65.83%), Query Frame = 0

Query: 27  CDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFFHKAAGRGCDGRHIIDFIANHLELPY 86
           C FPAI+NFGDSNSDTGG+SAAF     P G +FF   AGR CDGR +IDFIA  L LPY
Sbjct: 26  CHFPAIFNFGDSNSDTGGLSAAFGQAGPPHGSSFFGSPAGRYCDGRLVIDFIAESLGLPY 85

Query: 87  LSAYLNSIGANFRHGANFATGGSTIRRQNESVFLNGASPFSLDIQVVQFRQFKNRTIDLY 146
           LSA+L+S+G+NF HGANFAT GS IR  N ++  +G SPFSLD+Q VQF  F NR+  + 
Sbjct: 86  LSAFLDSVGSNFSHGANFATAGSPIRALNSTLRQSGFSPFSLDVQFVQFYNFHNRSQTVR 145

Query: 147 REATAEKSIRSTLPVPEDFSKALFTMDIGQNDLVAG-FRKMTNEQLRSVIPDIIGEFATA 206
                 K++   LP  + FSKAL+T DIGQNDL AG F   T EQ+ + +P+II +F  A
Sbjct: 146 SRGGVYKTM---LPESDSFSKALYTFDIGQNDLTAGYFANKTVEQVETEVPEIISQFMNA 205

Query: 207 VEDLYKEGARAFWVHNSGPIGCIPVAIR--SSVPGDLDKNGCVKYQNDAAAEFNTQLKER 266
           ++++Y +G R FW+HN+GPIGC+   I    +   D D +GCV   N  A +FN  LK+ 
Sbjct: 206 IKNIYGQGGRYFWIHNTGPIGCLAYVIERFPNKASDFDSHGCVSPLNHLAQQFNHALKQA 265

Query: 267 LLQLRANLSDVSLVYVDVFAAKTKLISNAKEEGFRETGAICCGYHEGYNH---VWCGNRA 326
           +++LR++LS+ ++ YVDV++ K +L  +A+  GF+ +   CCG+   YN+   + CG + 
Sbjct: 266 VIELRSSLSEAAITYVDVYSLKHELFVHAQGHGFKGSLVSCCGHGGKYNYNKGIGCGMKK 325

Query: 327 VINGSDVYAGS-CPDPSKFISWDGVHYTEAANRWIADRILHGYFSDPQVPITHACRR 377
           ++ G +VY G  C +P K + WDGVH+T+AAN++I D+I  G        ++ AC+R
Sbjct: 326 IVKGKEVYIGKPCDEPDKAVVWDGVHFTQAANKFIFDKIAPG--------LSKACKR 371

BLAST of MC08g_new0200 vs. TAIR 10
Match: AT3G27950.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 337.4 bits (864), Expect = 1.5e-92
Identity = 182/374 (48.66%), Postives = 231/374 (61.76%), Query Frame = 0

Query: 2   KIQMAFTVAWILLSAAWISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFF 61
           KI +A  V  +LL       A S +C+FPA++NFGDSNSDTG ISAA      P G  FF
Sbjct: 5   KITLAIIV--LLLGFTEKLSALSSSCNFPAVFNFGDSNSDTGAISAAIGEVPPPNGVAFF 64

Query: 62  HKAAGRGCDGRHIIDFIANHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFLN 121
            ++AGR  DGR IIDFI  +L LPYL+ YL+S+GAN+RHGANFATGGS IR       L 
Sbjct: 65  GRSAGRHSDGRLIIDFITENLTLPYLTPYLDSVGANYRHGANFATGGSCIRPT-----LA 124

Query: 122 GASPFSLDIQVVQFRQFKNRTIDLYREATAEKSIRSTLPVPEDFSKALFTMDIGQNDLVA 181
             SPF L  QV QF  FK RT+ LY +               DFSKAL+T+DIGQNDL  
Sbjct: 125 CFSPFHLGTQVSQFIHFKTRTLSLYNQT-------------NDFSKALYTLDIGQNDLAI 184

Query: 182 GFRKMTNEQLRSVIPDIIGEFATAVEDLYKEGARAFWVHNSGPIGCIPVAIRSSVPGDLD 241
           GF+ MT EQL++ IP II  F  A++ LYKEGAR F +HN+GP GC+P  +++      D
Sbjct: 185 GFQNMTEEQLKATIPLIIENFTIALKLLYKEGARFFSIHNTGPTGCLPYLLKAFPAIPRD 244

Query: 242 KNGCVKYQNDAAAEFNTQLKERLLQLRANLSDVSLVYVDVFAAKTKLISNAKEEGFRETG 301
             GC+K  N+ A EFN QLK ++ QL+  L      YVDV++AK  LI+ AK  GF +  
Sbjct: 245 PYGCLKPLNNVAIEFNKQLKNKITQLKKELPSSFFTYVDVYSAKYNLITKAKALGFIDPF 304

Query: 302 AICCGYHEGYNHVWCGNRAVINGSDVYAGSCPDPSKFISWDGVHYTEAANRWIADRILHG 361
             CC    G   + CG    +NG+++Y+ SC +   FISWDG+HYTE AN  +A+RIL G
Sbjct: 305 DYCCVGAIG-RGMGCGKTIFLNGTELYSSSCQNRKNFISWDGIHYTETANMLVANRILDG 357

Query: 362 YFSDPQVPITHACR 376
             SDP +P   AC+
Sbjct: 365 SISDPPLPTQKACK 357

BLAST of MC08g_new0200 vs. TAIR 10
Match: AT4G01130.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 278.9 bits (712), Expect = 6.2e-75
Identity = 151/375 (40.27%), Postives = 214/375 (57.07%), Query Frame = 0

Query: 6   AFTVAWILLSAAWISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFFHKAA 65
           +F++  +++   +    DS+ CDF AI+NFGDSNSDTGG  AAF     P G T+F K A
Sbjct: 10  SFSLLVLIIVMLYGHKGDSK-CDFEAIFNFGDSNSDTGGFWAAFPAQSGPWGMTYFKKPA 69

Query: 66  GRGCDGRHIIDFIANHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFLNGASP 125
           GR  DGR IIDF+A  L +P+LS YL SIG++FRHGANFAT  ST+   N S+F++G SP
Sbjct: 70  GRASDGRLIIDFLAKSLGMPFLSPYLQSIGSDFRHGANFATLASTVLLPNTSLFVSGISP 129

Query: 126 FSLDIQVVQFRQFKNRTIDLYREATAEKSIRSTLPVPEDFSKALFTMDIGQNDLVAGFRK 185
           FSL IQ+ Q +QFK   +++    + ++     LP    F K+L+T  IGQND  +    
Sbjct: 130 FSLAIQLNQMKQFK---VNVDESHSLDRPGLKILPSKIVFGKSLYTFYIGQNDFTSNLAS 189

Query: 186 MTNEQLRSVIPDIIGEFATAVEDLYKEGARAFWVHNSGPIGCIPVAIR--SSVPGDLDKN 245
           +  E+++  +P +IG+ A  ++++Y  G R F V N  P+GC P  +   +    DLDK 
Sbjct: 190 IGVERVKLYLPQVIGQIAGTIKEIYGIGGRTFLVLNLAPVGCYPAILTGYTHTDADLDKY 249

Query: 246 GCVKYQNDAAAEFNTQLKERLLQLRANLSDVSLVYVDVFAAKTKLISNAKEEGFRETGAI 305
           GC+   N A   +NT L + L Q R  L + +++Y+D       L  + K  G +     
Sbjct: 250 GCLIPVNKAVKYYNTLLNKTLSQTRTELKNATVIYLDTHKILLDLFQHPKSYGMKHGIKA 309

Query: 306 CCGY-HEGYN---HVWCGNRAVINGSDVYAGSCPDPSKFISWDGVHYTEAANRWIADRIL 365
           CCGY    YN    ++CGN  VI      A +C DP  ++SWDG+H TEAAN  I+  IL
Sbjct: 310 CCGYGGRPYNFNQKLFCGNTKVIGNFSTTAKACHDPHNYVSWDGIHATEAANHHISMAIL 369

Query: 366 HGYFSDPQVPITHAC 375
            G  S P   + + C
Sbjct: 370 DGSISYPPFILNNLC 380