MC08g_new0037 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g_new0037
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionAcetylglutamate kinase
LocationMC08: 2847016 .. 2848861 (+)
RNA-Seq ExpressionMC08g_new0037
SyntenyMC08g_new0037
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAATAACCAAATAATTGTGCATATTGTCGGAGAAAAAAAAGGGGAAAAACACTAAATAATTGTTCTTCAATTTCATATTATTGTTGTCCTTTTCAAAACCTCAAATTGTATTAATCAAATCAGAAAAAGAAATTCTTATAAACTGAGTTATCTGTTTCCTTACTGGGCTGCTCTTGCTCTAAAGCCCTAGTTGCCCTTTCCAATCCCCTGCTTCAAATCGCCGCCTCCCTCCGATCTCCGTCGCCGGCCGGGAAAATGCCGACGCTCGAAAGCTTGCTCAGTCCATCCGCCTCGAACCTATTTTCCTCCAAAACCTCGATTAATCCTTCCATTTCCAGTTCATCGGTTTTCTCGCCCGCTTTGCGTTTGCGCAAATTGGCGTCGTCTTCTTCGTCGCTGTCGATCAGATGCTCGGCGACACAGCAACTGGAGCCTGTTGTTGATTCTGTTACTCCGGGGCAGTTCCGAGTCGATGTTCTATCGGAATCCCTGCCTTTTATCCAGAAATTTCGAGGTAAGACAATCGTCGTCAAGTACGGAGGCGCCGCCATGAAATCTCGGTCGCTACAGGCCTCGGTTGTTAACGATCTCGTTCTTCTGTCCTGCGTTGGACTTCGTCCGATCTTGGTTCATGGCGGTGGGCCGGAAATTAACAACTGGCTTAAACGGCTCAACATCGAGGTGGTTTTCCGCGACGGACTGCGGGTGACCGACGCCGACACCATGAAAATAGTGTCTATGGTATTGGTCGGCGATGTGAACAAGAATCTGGTTTCGTTGATCAATAAGGCTGGGGCATCGGCCGCTGGGCTCTGTGGTGTGGACGGGCGGCTACTAACCGCCCGGCCAGCCCCTAATGCGGCACAATTAGGGTTCGTCGGCGAAGTAGCCCGCGTGGACCCTACGGTACTTCAGGCACTTCTGGACAAAGGCCACATTCCAGTGGTCTCTTCGGTGGCGGCGGACGAGTCCGGGCAGATGTACAACATCAACGCGGACACAGTCGCCGGAGAATTAGCAGCGGCGCTGGGGGCTGAGAAATTGATCTTGCTGACCGATGTGGCCGGGATTCTGGAAGACCGAGACGACCCTAAAAGCTTGGTGAAGCAGATAGATATTAAAGGGGTGAAGAAGATGATGGAGGAGGGAAGAATCGGCGGAGGTATGATTCCGAAGGTGAGTTGCTGTGTCCGATCGTTGGCTCAGGGGGTGAGGACGGCAAGTATCATCGACGGGAGGCTGCCGCATTCCTTGCTGCTGGAGATCATGACCGACAATGGAGCCGGAACCATGATTACAGGGTGACACCATGAGAGGTTTGTTTCAACTCCTAGATCTGTCTGGGAAGTGTATAAAATTTTTAAATGAACTCTGGAAAACAGAGTTGGATAGAAATTGTATGAGTAATTGCGATTTGATTCGCCTGAGTCTGAATCTCTGATCAAATCTGAATGTGTTGATTAATAAAGTTATAAGAGATTTTCATTCTTCTGGTGCAAGAACATGGAGTTTTAGAATTTGATTTTTCTCAGCATTGCCTCGTGCGCATTGGAATGAGTTTATGACTCTTTGCATAAGCCGTAGCCATTTGTGCAAAGAAAAAATAATTGGCTCACAGTGAGAGTACAATCTTCCTTACTTTAATTGGATAAAGAAATGAGTTGAAAGTGATTAGGTAGAAATATAACTAGAATCTTTTCTTCTCTTAGCAAATAGTTAGTTTAAATCCCATGTGATCTTAGTAAACCTGTTTGTTTTATTTTAGACATTCTGTCACCATTAGGTTAGGGTAGGTGTTCGAGTTTTGTTTTATTAAGATTAAATTACAAGTTTAGTTTATGGACA

mRNA sequence

ATGCCGACGCTCGAAAGCTTGCTCAGTCCATCCGCCTCGAACCTATTTTCCTCCAAAACCTCGATTAATCCTTCCATTTCCAGTTCATCGGTTTTCTCGCCCGCTTTGCGTTTGCGCAAATTGGCGTCGTCTTCTTCGTCGCTGTCGATCAGATGCTCGGCGACACAGCAACTGGAGCCTGTTGTTGATTCTGTTACTCCGGGGCAGTTCCGAGTCGATGTTCTATCGGAATCCCTGCCTTTTATCCAGAAATTTCGAGGTAAGACAATCGTCGTCAAGTACGGAGGCGCCGCCATGAAATCTCGGTCGCTACAGGCCTCGGTTGTTAACGATCTCGTTCTTCTGTCCTGCGTTGGACTTCGTCCGATCTTGGTTCATGGCGGTGGGCCGGAAATTAACAACTGGCTTAAACGGCTCAACATCGAGGTGGTTTTCCGCGACGGACTGCGGGTGACCGACGCCGACACCATGAAAATAGTGTCTATGGTATTGGTCGGCGATGTGAACAAGAATCTGGTTTCGTTGATCAATAAGGCTGGGGCATCGGCCGCTGGGCTCTGTGGTGTGGACGGGCGGCTACTAACCGCCCGGCCAGCCCCTAATGCGGCACAATTAGGGTTCGTCGGCGAAGTAGCCCGCGTGGACCCTACGGTACTTCAGGCACTTCTGGACAAAGGCCACATTCCAGTGGTCTCTTCGGTGGCGGCGGACGAGTCCGGGCAGATGTACAACATCAACGCGGACACAGTCGCCGGAGAATTAGCAGCGGCGCTGGGGGCTGAGAAATTGATCTTGCTGACCGATGTGGCCGGGATTCTGGAAGACCGAGACGACCCTAAAAGCTTGGTGAAGCAGATAGATATTAAAGGGGTGAAGAAGATGATGGAGGAGGGAAGAATCGGCGGAGGTATGATTCCGAAGGTGAGTTGCTGTGTCCGATCGTTGGCTCAGGGGGTGAGGACGGCAAGTATCATCGACGGGAGGCTGCCGCATTCCTTGCTGCTGGAGATCATGACCGACAATGGAGCCGGAACCATGATTACAGGGTGA

Coding sequence (CDS)

ATGCCGACGCTCGAAAGCTTGCTCAGTCCATCCGCCTCGAACCTATTTTCCTCCAAAACCTCGATTAATCCTTCCATTTCCAGTTCATCGGTTTTCTCGCCCGCTTTGCGTTTGCGCAAATTGGCGTCGTCTTCTTCGTCGCTGTCGATCAGATGCTCGGCGACACAGCAACTGGAGCCTGTTGTTGATTCTGTTACTCCGGGGCAGTTCCGAGTCGATGTTCTATCGGAATCCCTGCCTTTTATCCAGAAATTTCGAGGTAAGACAATCGTCGTCAAGTACGGAGGCGCCGCCATGAAATCTCGGTCGCTACAGGCCTCGGTTGTTAACGATCTCGTTCTTCTGTCCTGCGTTGGACTTCGTCCGATCTTGGTTCATGGCGGTGGGCCGGAAATTAACAACTGGCTTAAACGGCTCAACATCGAGGTGGTTTTCCGCGACGGACTGCGGGTGACCGACGCCGACACCATGAAAATAGTGTCTATGGTATTGGTCGGCGATGTGAACAAGAATCTGGTTTCGTTGATCAATAAGGCTGGGGCATCGGCCGCTGGGCTCTGTGGTGTGGACGGGCGGCTACTAACCGCCCGGCCAGCCCCTAATGCGGCACAATTAGGGTTCGTCGGCGAAGTAGCCCGCGTGGACCCTACGGTACTTCAGGCACTTCTGGACAAAGGCCACATTCCAGTGGTCTCTTCGGTGGCGGCGGACGAGTCCGGGCAGATGTACAACATCAACGCGGACACAGTCGCCGGAGAATTAGCAGCGGCGCTGGGGGCTGAGAAATTGATCTTGCTGACCGATGTGGCCGGGATTCTGGAAGACCGAGACGACCCTAAAAGCTTGGTGAAGCAGATAGATATTAAAGGGGTGAAGAAGATGATGGAGGAGGGAAGAATCGGCGGAGGTATGATTCCGAAGGTGAGTTGCTGTGTCCGATCGTTGGCTCAGGGGGTGAGGACGGCAAGTATCATCGACGGGAGGCTGCCGCATTCCTTGCTGCTGGAGATCATGACCGACAATGGAGCCGGAACCATGATTACAGGGTGA

Protein sequence

MPTLESLLSPSASNLFSSKTSINPSISSSSVFSPALRLRKLASSSSSLSIRCSATQQLEPVVDSVTPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLRPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDADTMKIVSMVLVGDVNKNLVSLINKAGASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIGGGMIPKVSCCVRSLAQGVRTASIIDGRLPHSLLLEIMTDNGAGTMITG
Homology
BLAST of MC08g_new0037 vs. ExPASy Swiss-Prot
Match: Q9SCL7 (Acetylglutamate kinase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NAGK PE=1 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 1.5e-120
Identity = 240/351 (68.38%), Postives = 279/351 (79.49%), Query Frame = 0

Query: 1   MPTLESLLSPSASNLFSSKTSINPSISSSSVFSPALRLRKLASSSSSLSIRCSATQQLEP 60
           M T+ S  SP +     S T  NP  +     SP+L       +   L     AT    P
Sbjct: 1   MATVTSNASPKS----FSFTVSNPFKTLIPNKSPSLCYPTRNKNHHRLGFSIKATVSTPP 60

Query: 61  VV--DSVTPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCV 120
            +   +     +RV++LSESLPFIQKFRGKTIVVKYGGAAM S  L++SVV+DLVLL+CV
Sbjct: 61  SIATGNAPSPDYRVEILSESLPFIQKFRGKTIVVKYGGAAMTSPELKSSVVSDLVLLACV 120

Query: 121 GLRPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDADTMKIVSMVLVGDVNKNLVSLINK 180
           GLRPILVHGGGP+IN +LK+LNI   FRDGLRVTDA TM+IVSMVLVG VNKNLVSLIN 
Sbjct: 121 GLRPILVHGGGPDINRYLKQLNIPAEFRDGLRVTDATTMEIVSMVLVGKVNKNLVSLINA 180

Query: 181 AGASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADE 240
           AGA+A GL G DGRLLTARP PN+AQLGFVGEVARVDP+VL+ L+D G+IPV++SVAAD+
Sbjct: 181 AGATAVGLSGHDGRLLTARPVPNSAQLGFVGEVARVDPSVLRPLVDYGYIPVIASVAADD 240

Query: 241 SGQMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEG 300
           SGQ YNINADTVAGELAAALGAEKLILLTDVAGILE+++DP SL+K+IDIKGVKKM+E+G
Sbjct: 241 SGQAYNINADTVAGELAAALGAEKLILLTDVAGILENKEDPSSLIKEIDIKGVKKMIEDG 300

Query: 301 RIGGGMIPKVSCCVRSLAQGVRTASIIDGRLPHSLLLEIMTDNGAGTMITG 350
           ++ GGMIPKV CC+RSLAQGV+TASIIDGR  HSLL EIM+D GAGTMITG
Sbjct: 301 KVAGGMIPKVKCCIRSLAQGVKTASIIDGRRQHSLLHEIMSDEGAGTMITG 347

BLAST of MC08g_new0037 vs. ExPASy Swiss-Prot
Match: Q8YXG8 (Acetylglutamate kinase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=argB PE=3 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 5.9e-101
Identity = 186/279 (66.67%), Postives = 224/279 (80.29%), Query Frame = 0

Query: 71  RVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLRPILVHGGGP 130
           RV VLSE+LP+IQ+F G+T+VVKYGGAAMK   L+  V+ D+V LSCVGLRPILVHGGGP
Sbjct: 16  RVQVLSEALPYIQQFAGRTVVVKYGGAAMKDSHLKDQVIRDIVFLSCVGLRPILVHGGGP 75

Query: 131 EINNWLKRLNIEVVFRDGLRVTDADTMKIVSMVLVGDVNKNLVSLINKAGASAAGLCGVD 190
           EIN+WL +L IE  F++GLRVTDA TM +V MVLVG VNK +VSLIN+AG  A GLCG D
Sbjct: 76  EINSWLDKLGIEAQFKNGLRVTDAPTMDVVEMVLVGRVNKEIVSLINQAGGLAVGLCGKD 135

Query: 191 GRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQMYNINADTV 250
           G L+TARP      +GFVGEV+ V+  +L+ L   G+IPVVSSVAAD+SGQ YNINADTV
Sbjct: 136 GNLITARPQGQEG-IGFVGEVSNVNIKILETLASNGYIPVVSSVAADDSGQAYNINADTV 195

Query: 251 AGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIGGGMIPKVSC 310
           AGE+AAALGAEKLILLTD  GIL+D  DP +L+ ++DI+  ++++  G + GGMIPKV+C
Sbjct: 196 AGEIAAALGAEKLILLTDTRGILKDYQDPGTLIPKVDIREARELINSGVVSGGMIPKVTC 255

Query: 311 CVRSLAQGVRTASIIDGRLPHSLLLEIMTDNGAGTMITG 350
           CVRSLAQGVR A IIDGR+PH+LLLEI TD G GTMI G
Sbjct: 256 CVRSLAQGVRAAHIIDGRIPHALLLEIFTDVGIGTMILG 293

BLAST of MC08g_new0037 vs. ExPASy Swiss-Prot
Match: Q3MFQ8 (Acetylglutamate kinase OS=Trichormus variabilis (strain ATCC 29413 / PCC 7937) OX=240292 GN=argB PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.3e-100
Identity = 186/279 (66.67%), Postives = 224/279 (80.29%), Query Frame = 0

Query: 71  RVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLRPILVHGGGP 130
           RV VLSE+LP+IQ+F G+T+VVKYGGAAMK   L+  V+ D+V LSCVGLRPILVHGGGP
Sbjct: 16  RVQVLSEALPYIQQFAGRTVVVKYGGAAMKDSHLKDQVIRDIVFLSCVGLRPILVHGGGP 75

Query: 131 EINNWLKRLNIEVVFRDGLRVTDADTMKIVSMVLVGDVNKNLVSLINKAGASAAGLCGVD 190
           EIN+WL +L IE  F++GLRVTDA TM +V MVLVG VNK +VSLIN+AG  A GLCG D
Sbjct: 76  EINSWLDKLGIEAQFKNGLRVTDAPTMDVVEMVLVGRVNKEIVSLINQAGGLAVGLCGKD 135

Query: 191 GRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQMYNINADTV 250
           G L+TARP      +GFVGEV+ V+  +L+ L   G+IPVVSSVAAD+SGQ YNINADTV
Sbjct: 136 GNLITARPQGQEG-IGFVGEVSNVNIKILETLASNGYIPVVSSVAADDSGQAYNINADTV 195

Query: 251 AGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIGGGMIPKVSC 310
           AGE+AAALGAEKLILLTD  GIL+D  DP +L+ ++DI+  ++++  G + GGMIPKV+C
Sbjct: 196 AGEIAAALGAEKLILLTDTRGILKDYKDPGTLIPKVDIREARELINGGVVSGGMIPKVTC 255

Query: 311 CVRSLAQGVRTASIIDGRLPHSLLLEIMTDNGAGTMITG 350
           CVRSLAQGVR A IIDGR+PH+LLLEI TD G GTMI G
Sbjct: 256 CVRSLAQGVRAAHIIDGRIPHALLLEIFTDVGIGTMILG 293

BLAST of MC08g_new0037 vs. ExPASy Swiss-Prot
Match: B0CAM3 (Acetylglutamate kinase OS=Acaryochloris marina (strain MBIC 11017) OX=329726 GN=argB PE=3 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 3.8e-100
Identity = 184/278 (66.19%), Postives = 225/278 (80.94%), Query Frame = 0

Query: 71  RVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLRPILVHGGGP 130
           RV VLSE+LP+IQ F G+T+VVKYGGAAMK  +L+A+V+ D+V ++CVGLRP+LVHGGGP
Sbjct: 7   RVQVLSEALPYIQSFAGRTVVVKYGGAAMKDSTLKATVIRDVVFMACVGLRPVLVHGGGP 66

Query: 131 EINNWLKRLNIEVVFRDGLRVTDADTMKIVSMVLVGDVNKNLVSLINKAGASAAGLCGVD 190
           EIN WL++L IE  F  GLRVTDA TM +V MVLVG VNK LVSLIN+AG SA G+CG D
Sbjct: 67  EINLWLEKLGIEPQFMKGLRVTDAPTMDVVEMVLVGRVNKELVSLINQAGGSAVGVCGKD 126

Query: 191 GRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQMYNINADTV 250
           G+LLTARP   +  +GFVGEVA V+P ++  LLD GHIP+VSSVA DE GQ+YNINADTV
Sbjct: 127 GKLLTARPQGESG-IGFVGEVASVNPQLIHNLLDGGHIPIVSSVATDEEGQVYNINADTV 186

Query: 251 AGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIGGGMIPKVSC 310
           AGELAAAL AEKLILLTD  GIL+D  D  SL+ ++DI+  ++++    + GGMIPKV+C
Sbjct: 187 AGELAAALDAEKLILLTDTPGILQDYKDATSLIHKLDIRQARELIAAEVVAGGMIPKVTC 246

Query: 311 CVRSLAQGVRTASIIDGRLPHSLLLEIMTDNGAGTMIT 349
           C+RSLAQG++ A IIDGR PH+LLLEI TD+G GTMIT
Sbjct: 247 CIRSLAQGIQAAHIIDGRAPHALLLEIFTDSGIGTMIT 283

BLAST of MC08g_new0037 vs. ExPASy Swiss-Prot
Match: B0JHB1 (Acetylglutamate kinase OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=argB PE=3 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 8.5e-100
Identity = 177/277 (63.90%), Postives = 231/277 (83.39%), Query Frame = 0

Query: 71  RVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLRPILVHGGGP 130
           RV +LSE+LP+IQ+FRG+T+VVKYGGAAM +  ++ +V+ D+V LSCVG+RP++VHGGGP
Sbjct: 12  RVKILSEALPYIQEFRGRTVVVKYGGAAMNNSDIKDTVMRDIVFLSCVGVRPVIVHGGGP 71

Query: 131 EINNWLKRLNIEVVFRDGLRVTDADTMKIVSMVLVGDVNKNLVSLINKAGASAAGLCGVD 190
           EIN+WL +L IE  F+DGLRVTDADTM +V MVLVG VNK +V+LIN+AG  A GLCG D
Sbjct: 72  EINSWLDKLGIEPQFKDGLRVTDADTMDVVEMVLVGRVNKEIVALINQAGGKAVGLCGKD 131

Query: 191 GRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQMYNINADTV 250
           G L+TARP      +GFVGEV+ VD +V+++L++ G+IPV+SSVAADE+GQ +NINADTV
Sbjct: 132 GNLITARPVGKEG-IGFVGEVSSVDTSVVESLVNSGYIPVISSVAADETGQAHNINADTV 191

Query: 251 AGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIGGGMIPKVSC 310
           AGE+AAALGA KLIL+TD AGIL++  DP +L+ ++DI+  ++++++G + GGMIPKV C
Sbjct: 192 AGEIAAALGAAKLILMTDTAGILQNYKDPSTLLAKLDIQQARELIQKGIVAGGMIPKVGC 251

Query: 311 CVRSLAQGVRTASIIDGRLPHSLLLEIMTDNGAGTMI 348
           CVRSLAQGV+ A IIDGRLPH+LLLEI TD G G+MI
Sbjct: 252 CVRSLAQGVQAAHIIDGRLPHALLLEIFTDRGIGSMI 287

BLAST of MC08g_new0037 vs. NCBI nr
Match: XP_022131382.1 (acetylglutamate kinase, chloroplastic [Momordica charantia])

HSP 1 Score: 656 bits (1693), Expect = 5.42e-237
Identity = 349/349 (100.00%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 1   MPTLESLLSPSASNLFSSKTSINPSISSSSVFSPALRLRKLASSSSSLSIRCSATQQLEP 60
           MPTLESLLSPSASNLFSSKTSINPSISSSSVFSPALRLRKLASSSSSLSIRCSATQQLEP
Sbjct: 1   MPTLESLLSPSASNLFSSKTSINPSISSSSVFSPALRLRKLASSSSSLSIRCSATQQLEP 60

Query: 61  VVDSVTPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGL 120
           VVDSVTPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGL
Sbjct: 61  VVDSVTPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGL 120

Query: 121 RPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDADTMKIVSMVLVGDVNKNLVSLINKAG 180
           RPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDADTMKIVSMVLVGDVNKNLVSLINKAG
Sbjct: 121 RPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDADTMKIVSMVLVGDVNKNLVSLINKAG 180

Query: 181 ASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESG 240
           ASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESG
Sbjct: 181 ASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESG 240

Query: 241 QMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRI 300
           QMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRI
Sbjct: 241 QMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRI 300

Query: 301 GGGMIPKVSCCVRSLAQGVRTASIIDGRLPHSLLLEIMTDNGAGTMITG 349
           GGGMIPKVSCCVRSLAQGVRTASIIDGRLPHSLLLEIMTDNGAGTMITG
Sbjct: 301 GGGMIPKVSCCVRSLAQGVRTASIIDGRLPHSLLLEIMTDNGAGTMITG 349

BLAST of MC08g_new0037 vs. NCBI nr
Match: XP_038886528.1 (acetylglutamate kinase, chloroplastic [Benincasa hispida])

HSP 1 Score: 624 bits (1608), Expect = 4.87e-224
Identity = 329/349 (94.27%), Postives = 341/349 (97.71%), Query Frame = 0

Query: 1   MPTLESLLSPSASNLFSSKTSINPSISSSSVFSPALRLRKLASSSSSLSIRCSATQQLEP 60
           MP +ESLL+PSAS LF+SK+ +NPSISSSS+FSPA RLR+L+ SSSSLSIRCSATQQ+E 
Sbjct: 1   MPAVESLLNPSASKLFTSKSLLNPSISSSSIFSPAPRLRRLSCSSSSLSIRCSATQQIEA 60

Query: 61  VVDSVTPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGL 120
           VVDSVTPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGL
Sbjct: 61  VVDSVTPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGL 120

Query: 121 RPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDADTMKIVSMVLVGDVNKNLVSLINKAG 180
           RPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDA+TMKIVSMVLVGDVNKNLVSLINKAG
Sbjct: 121 RPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAG 180

Query: 181 ASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESG 240
           ASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESG
Sbjct: 181 ASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESG 240

Query: 241 QMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRI 300
           QMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMM+EGRI
Sbjct: 241 QMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMDEGRI 300

Query: 301 GGGMIPKVSCCVRSLAQGVRTASIIDGRLPHSLLLEIMTDNGAGTMITG 349
           GGGMIPKV+CCVRSLAQGVRTASIIDGRL HSLLLEIMTDNGAGTMI G
Sbjct: 301 GGGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 349

BLAST of MC08g_new0037 vs. NCBI nr
Match: XP_022929565.1 (acetylglutamate kinase, chloroplastic-like [Cucurbita moschata] >KAG6598822.1 Acetylglutamate kinase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029767.1 Acetylglutamate kinase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 619 bits (1597), Expect = 2.22e-222
Identity = 331/349 (94.84%), Postives = 340/349 (97.42%), Query Frame = 0

Query: 1   MPTLESLLSPSASNLFSSKTSINPSISSSSVFSPALRLRKLASSSSSLSIRCSATQQLEP 60
           M T+ESLL+PSAS LFSSK+  NPSISSSS+FSPA RLR+L SSSSSLSIRCSATQQ+E 
Sbjct: 1   MSTVESLLNPSASKLFSSKSLFNPSISSSSIFSPAPRLRRL-SSSSSLSIRCSATQQMEA 60

Query: 61  VVDSVTPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGL 120
           VVDSV+PGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGL
Sbjct: 61  VVDSVSPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGL 120

Query: 121 RPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDADTMKIVSMVLVGDVNKNLVSLINKAG 180
           RPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDA+TMKIVSMVLVGDVNKNLVSLINKAG
Sbjct: 121 RPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAG 180

Query: 181 ASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESG 240
           ASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESG
Sbjct: 181 ASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESG 240

Query: 241 QMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRI 300
           QMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMM+EGRI
Sbjct: 241 QMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMDEGRI 300

Query: 301 GGGMIPKVSCCVRSLAQGVRTASIIDGRLPHSLLLEIMTDNGAGTMITG 349
           GGGMIPKVSCCVRSLAQGVRTASIIDGRL HSLLLEIMTDNGAGTMI G
Sbjct: 301 GGGMIPKVSCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 348

BLAST of MC08g_new0037 vs. NCBI nr
Match: XP_022932833.1 (acetylglutamate kinase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 618 bits (1594), Expect = 6.37e-222
Identity = 330/349 (94.56%), Postives = 339/349 (97.13%), Query Frame = 0

Query: 1   MPTLESLLSPSASNLFSSKTSINPSISSSSVFSPALRLRKLASSSSSLSIRCSATQQLEP 60
           MPT+ESLL+PSA NLFSSK+  NPSISSSS+FSPA RLR+L SSSSSLSIRCSATQQ+E 
Sbjct: 1   MPTIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRL-SSSSSLSIRCSATQQMEA 60

Query: 61  VVDSVTPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGL 120
           VVDSV+P QFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGL
Sbjct: 61  VVDSVSPEQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGL 120

Query: 121 RPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDADTMKIVSMVLVGDVNKNLVSLINKAG 180
           RPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDA+TMKIVSMVLVGDVNKNLVSLINKAG
Sbjct: 121 RPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAG 180

Query: 181 ASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESG 240
           ASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIP VSSVAADESG
Sbjct: 181 ASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPAVSSVAADESG 240

Query: 241 QMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRI 300
           QMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRI
Sbjct: 241 QMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRI 300

Query: 301 GGGMIPKVSCCVRSLAQGVRTASIIDGRLPHSLLLEIMTDNGAGTMITG 349
           GGGMIPKV+CCVRSLAQGVRTASIIDGRL HSLLLEIMTDNGAGTMI G
Sbjct: 301 GGGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 348

BLAST of MC08g_new0037 vs. NCBI nr
Match: XP_004138756.1 (acetylglutamate kinase, chloroplastic [Cucumis sativus] >KGN62888.1 hypothetical protein Csa_022602 [Cucumis sativus])

HSP 1 Score: 618 bits (1593), Expect = 1.01e-221
Identity = 329/351 (93.73%), Postives = 340/351 (96.87%), Query Frame = 0

Query: 1   MPTLESLLSPSASNLFSSKTSINPSISSSSVFSPALRLRKL--ASSSSSLSIRCSATQQL 60
           MP +ESLLSPSAS LF+SK+ +NPSISSSS+FSPA R R+L  +SSSSSLSIRCSATQQ+
Sbjct: 1   MPAVESLLSPSASKLFTSKSLLNPSISSSSIFSPAPRFRRLPSSSSSSSLSIRCSATQQM 60

Query: 61  EPVVDSVTPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCV 120
           E VVDSVTPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCV
Sbjct: 61  EAVVDSVTPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCV 120

Query: 121 GLRPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDADTMKIVSMVLVGDVNKNLVSLINK 180
           GLRPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDA+TMKIVSMVLVGDVNKNLVSLINK
Sbjct: 121 GLRPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDAETMKIVSMVLVGDVNKNLVSLINK 180

Query: 181 AGASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADE 240
           AGASAAGLCGVDGRLL ARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADE
Sbjct: 181 AGASAAGLCGVDGRLLMARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADE 240

Query: 241 SGQMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEG 300
           SGQMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMM+EG
Sbjct: 241 SGQMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMDEG 300

Query: 301 RIGGGMIPKVSCCVRSLAQGVRTASIIDGRLPHSLLLEIMTDNGAGTMITG 349
           RIGGGMIPKV+CCVRSLAQGVRTASIIDGRL HSLLLEIMTDNGAGTMI G
Sbjct: 301 RIGGGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 351

BLAST of MC08g_new0037 vs. ExPASy TrEMBL
Match: A0A6J1BQV1 (acetylglutamate kinase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111004618 PE=3 SV=1)

HSP 1 Score: 656 bits (1693), Expect = 2.63e-237
Identity = 349/349 (100.00%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 1   MPTLESLLSPSASNLFSSKTSINPSISSSSVFSPALRLRKLASSSSSLSIRCSATQQLEP 60
           MPTLESLLSPSASNLFSSKTSINPSISSSSVFSPALRLRKLASSSSSLSIRCSATQQLEP
Sbjct: 1   MPTLESLLSPSASNLFSSKTSINPSISSSSVFSPALRLRKLASSSSSLSIRCSATQQLEP 60

Query: 61  VVDSVTPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGL 120
           VVDSVTPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGL
Sbjct: 61  VVDSVTPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGL 120

Query: 121 RPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDADTMKIVSMVLVGDVNKNLVSLINKAG 180
           RPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDADTMKIVSMVLVGDVNKNLVSLINKAG
Sbjct: 121 RPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDADTMKIVSMVLVGDVNKNLVSLINKAG 180

Query: 181 ASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESG 240
           ASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESG
Sbjct: 181 ASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESG 240

Query: 241 QMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRI 300
           QMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRI
Sbjct: 241 QMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRI 300

Query: 301 GGGMIPKVSCCVRSLAQGVRTASIIDGRLPHSLLLEIMTDNGAGTMITG 349
           GGGMIPKVSCCVRSLAQGVRTASIIDGRLPHSLLLEIMTDNGAGTMITG
Sbjct: 301 GGGMIPKVSCCVRSLAQGVRTASIIDGRLPHSLLLEIMTDNGAGTMITG 349

BLAST of MC08g_new0037 vs. ExPASy TrEMBL
Match: A0A6J1EUQ8 (acetylglutamate kinase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111436114 PE=3 SV=1)

HSP 1 Score: 619 bits (1597), Expect = 1.08e-222
Identity = 331/349 (94.84%), Postives = 340/349 (97.42%), Query Frame = 0

Query: 1   MPTLESLLSPSASNLFSSKTSINPSISSSSVFSPALRLRKLASSSSSLSIRCSATQQLEP 60
           M T+ESLL+PSAS LFSSK+  NPSISSSS+FSPA RLR+L SSSSSLSIRCSATQQ+E 
Sbjct: 1   MSTVESLLNPSASKLFSSKSLFNPSISSSSIFSPAPRLRRL-SSSSSLSIRCSATQQMEA 60

Query: 61  VVDSVTPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGL 120
           VVDSV+PGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGL
Sbjct: 61  VVDSVSPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGL 120

Query: 121 RPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDADTMKIVSMVLVGDVNKNLVSLINKAG 180
           RPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDA+TMKIVSMVLVGDVNKNLVSLINKAG
Sbjct: 121 RPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAG 180

Query: 181 ASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESG 240
           ASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESG
Sbjct: 181 ASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESG 240

Query: 241 QMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRI 300
           QMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMM+EGRI
Sbjct: 241 QMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMDEGRI 300

Query: 301 GGGMIPKVSCCVRSLAQGVRTASIIDGRLPHSLLLEIMTDNGAGTMITG 349
           GGGMIPKVSCCVRSLAQGVRTASIIDGRL HSLLLEIMTDNGAGTMI G
Sbjct: 301 GGGMIPKVSCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 348

BLAST of MC08g_new0037 vs. ExPASy TrEMBL
Match: A0A6J1F2V3 (acetylglutamate kinase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111439296 PE=3 SV=1)

HSP 1 Score: 618 bits (1594), Expect = 3.08e-222
Identity = 330/349 (94.56%), Postives = 339/349 (97.13%), Query Frame = 0

Query: 1   MPTLESLLSPSASNLFSSKTSINPSISSSSVFSPALRLRKLASSSSSLSIRCSATQQLEP 60
           MPT+ESLL+PSA NLFSSK+  NPSISSSS+FSPA RLR+L SSSSSLSIRCSATQQ+E 
Sbjct: 1   MPTIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRL-SSSSSLSIRCSATQQMEA 60

Query: 61  VVDSVTPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGL 120
           VVDSV+P QFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGL
Sbjct: 61  VVDSVSPEQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGL 120

Query: 121 RPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDADTMKIVSMVLVGDVNKNLVSLINKAG 180
           RPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDA+TMKIVSMVLVGDVNKNLVSLINKAG
Sbjct: 121 RPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAG 180

Query: 181 ASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESG 240
           ASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIP VSSVAADESG
Sbjct: 181 ASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPAVSSVAADESG 240

Query: 241 QMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRI 300
           QMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRI
Sbjct: 241 QMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRI 300

Query: 301 GGGMIPKVSCCVRSLAQGVRTASIIDGRLPHSLLLEIMTDNGAGTMITG 349
           GGGMIPKV+CCVRSLAQGVRTASIIDGRL HSLLLEIMTDNGAGTMI G
Sbjct: 301 GGGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 348

BLAST of MC08g_new0037 vs. ExPASy TrEMBL
Match: A0A0A0LM56 (AA_kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G379190 PE=3 SV=1)

HSP 1 Score: 618 bits (1593), Expect = 4.90e-222
Identity = 329/351 (93.73%), Postives = 340/351 (96.87%), Query Frame = 0

Query: 1   MPTLESLLSPSASNLFSSKTSINPSISSSSVFSPALRLRKL--ASSSSSLSIRCSATQQL 60
           MP +ESLLSPSAS LF+SK+ +NPSISSSS+FSPA R R+L  +SSSSSLSIRCSATQQ+
Sbjct: 1   MPAVESLLSPSASKLFTSKSLLNPSISSSSIFSPAPRFRRLPSSSSSSSLSIRCSATQQM 60

Query: 61  EPVVDSVTPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCV 120
           E VVDSVTPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCV
Sbjct: 61  EAVVDSVTPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCV 120

Query: 121 GLRPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDADTMKIVSMVLVGDVNKNLVSLINK 180
           GLRPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDA+TMKIVSMVLVGDVNKNLVSLINK
Sbjct: 121 GLRPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDAETMKIVSMVLVGDVNKNLVSLINK 180

Query: 181 AGASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADE 240
           AGASAAGLCGVDGRLL ARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADE
Sbjct: 181 AGASAAGLCGVDGRLLMARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADE 240

Query: 241 SGQMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEG 300
           SGQMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMM+EG
Sbjct: 241 SGQMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMDEG 300

Query: 301 RIGGGMIPKVSCCVRSLAQGVRTASIIDGRLPHSLLLEIMTDNGAGTMITG 349
           RIGGGMIPKV+CCVRSLAQGVRTASIIDGRL HSLLLEIMTDNGAGTMI G
Sbjct: 301 RIGGGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 351

BLAST of MC08g_new0037 vs. ExPASy TrEMBL
Match: A0A6J1K509 (acetylglutamate kinase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111492351 PE=3 SV=1)

HSP 1 Score: 617 bits (1591), Expect = 8.84e-222
Identity = 330/349 (94.56%), Postives = 339/349 (97.13%), Query Frame = 0

Query: 1   MPTLESLLSPSASNLFSSKTSINPSISSSSVFSPALRLRKLASSSSSLSIRCSATQQLEP 60
           M T+ESLL+PSAS LFS K+  NPSISSSS+FSPA RLR+L SSSSSLSIRCSATQQ+E 
Sbjct: 1   MSTVESLLNPSASKLFSFKSLFNPSISSSSIFSPAPRLRRL-SSSSSLSIRCSATQQMEA 60

Query: 61  VVDSVTPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGL 120
           VVDSV+PGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGL
Sbjct: 61  VVDSVSPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGL 120

Query: 121 RPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDADTMKIVSMVLVGDVNKNLVSLINKAG 180
           RPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDA+TMKIVSMVLVGDVNKNLVSLINKAG
Sbjct: 121 RPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAG 180

Query: 181 ASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESG 240
           ASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESG
Sbjct: 181 ASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESG 240

Query: 241 QMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRI 300
           QMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMM+EGRI
Sbjct: 241 QMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMDEGRI 300

Query: 301 GGGMIPKVSCCVRSLAQGVRTASIIDGRLPHSLLLEIMTDNGAGTMITG 349
           GGGMIPKVSCCVRSLAQGVRTASIIDGRL HSLLLEIMTDNGAGTMI G
Sbjct: 301 GGGMIPKVSCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 348

BLAST of MC08g_new0037 vs. TAIR 10
Match: AT3G57560.1 (N-acetyl-l-glutamate kinase )

HSP 1 Score: 434.1 bits (1115), Expect = 1.1e-121
Identity = 240/351 (68.38%), Postives = 279/351 (79.49%), Query Frame = 0

Query: 1   MPTLESLLSPSASNLFSSKTSINPSISSSSVFSPALRLRKLASSSSSLSIRCSATQQLEP 60
           M T+ S  SP +     S T  NP  +     SP+L       +   L     AT    P
Sbjct: 1   MATVTSNASPKS----FSFTVSNPFKTLIPNKSPSLCYPTRNKNHHRLGFSIKATVSTPP 60

Query: 61  VV--DSVTPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCV 120
            +   +     +RV++LSESLPFIQKFRGKTIVVKYGGAAM S  L++SVV+DLVLL+CV
Sbjct: 61  SIATGNAPSPDYRVEILSESLPFIQKFRGKTIVVKYGGAAMTSPELKSSVVSDLVLLACV 120

Query: 121 GLRPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDADTMKIVSMVLVGDVNKNLVSLINK 180
           GLRPILVHGGGP+IN +LK+LNI   FRDGLRVTDA TM+IVSMVLVG VNKNLVSLIN 
Sbjct: 121 GLRPILVHGGGPDINRYLKQLNIPAEFRDGLRVTDATTMEIVSMVLVGKVNKNLVSLINA 180

Query: 181 AGASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADE 240
           AGA+A GL G DGRLLTARP PN+AQLGFVGEVARVDP+VL+ L+D G+IPV++SVAAD+
Sbjct: 181 AGATAVGLSGHDGRLLTARPVPNSAQLGFVGEVARVDPSVLRPLVDYGYIPVIASVAADD 240

Query: 241 SGQMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEG 300
           SGQ YNINADTVAGELAAALGAEKLILLTDVAGILE+++DP SL+K+IDIKGVKKM+E+G
Sbjct: 241 SGQAYNINADTVAGELAAALGAEKLILLTDVAGILENKEDPSSLIKEIDIKGVKKMIEDG 300

Query: 301 RIGGGMIPKVSCCVRSLAQGVRTASIIDGRLPHSLLLEIMTDNGAGTMITG 350
           ++ GGMIPKV CC+RSLAQGV+TASIIDGR  HSLL EIM+D GAGTMITG
Sbjct: 301 KVAGGMIPKVKCCIRSLAQGVKTASIIDGRRQHSLLHEIMSDEGAGTMITG 347

BLAST of MC08g_new0037 vs. TAIR 10
Match: AT4G37670.2 (N-acetyl-l-glutamate synthase 2 )

HSP 1 Score: 55.1 bits (131), Expect = 1.3e-07
Identity = 70/276 (25.36%), Postives = 119/276 (43.12%), Query Frame = 0

Query: 9   SPSASNLFSSKTSINPS--ISSSSVFSPALRLRKLASSSSSLSIRCSATQQLEPVVDSVT 68
           S S  + F  K  +NP+    + S F P   +     +    ++  +   + E  VD   
Sbjct: 24  SKSNFSSFKPKNKLNPTQFRFNCSWFKPVSSITAAKCNMFDYAVTAAGDVEAEHPVDD-- 83

Query: 69  PGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLRPILVH 128
             QF V    E+ P++   RG T VV   G  +   S  A ++ D+  L  +G+R +LV 
Sbjct: 84  -KQF-VRWFREAWPYLWAHRGCTFVVIISGEIIAGSSCDA-ILKDIAFLHHLGIRFVLVP 143

Query: 129 GGGPEINNWLKRLNIEVVFRDGLRVTDADTMK-------IVSMVLVGDVN--KNLVSLIN 188
           G   +I+  L     E  +    RVTDA +++        +S++L   ++   ++ ++  
Sbjct: 144 GTQEQIDQLLAERGREATYVGRYRVTDAASLQAAKEAAGAISVMLEAKLSPGPSICNIRR 203

Query: 189 KAGASAAGLCGV---DGRLLTA--RPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVS 248
               S     GV    G    A  R   +    G  GEV ++D   +   LD G + ++ 
Sbjct: 204 HGDRSRLHDIGVRVDTGNFFAAKRRGVVDGVDFGATGEVKKIDVDRICERLDGGSVVLLR 263

Query: 249 SVAADESGQMYNINADTVAGELAAALGAEKLILLTD 269
           ++    SG++ N N   VA   A A+GA+KLI + D
Sbjct: 264 NLGHSSSGEVLNCNTYEVATACALAIGADKLICIMD 294

BLAST of MC08g_new0037 vs. TAIR 10
Match: AT4G37670.1 (N-acetyl-l-glutamate synthase 2 )

HSP 1 Score: 55.1 bits (131), Expect = 1.3e-07
Identity = 70/276 (25.36%), Postives = 119/276 (43.12%), Query Frame = 0

Query: 9   SPSASNLFSSKTSINPS--ISSSSVFSPALRLRKLASSSSSLSIRCSATQQLEPVVDSVT 68
           S S  + F  K  +NP+    + S F P   +     +    ++  +   + E  VD   
Sbjct: 24  SKSNFSSFKPKNKLNPTQFRFNCSWFKPVSSITAAKCNMFDYAVTAAGDVEAEHPVDD-- 83

Query: 69  PGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLRPILVH 128
             QF V    E+ P++   RG T VV   G  +   S  A ++ D+  L  +G+R +LV 
Sbjct: 84  -KQF-VRWFREAWPYLWAHRGCTFVVIISGEIIAGSSCDA-ILKDIAFLHHLGIRFVLVP 143

Query: 129 GGGPEINNWLKRLNIEVVFRDGLRVTDADTMK-------IVSMVLVGDVN--KNLVSLIN 188
           G   +I+  L     E  +    RVTDA +++        +S++L   ++   ++ ++  
Sbjct: 144 GTQEQIDQLLAERGREATYVGRYRVTDAASLQAAKEAAGAISVMLEAKLSPGPSICNIRR 203

Query: 189 KAGASAAGLCGV---DGRLLTA--RPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVS 248
               S     GV    G    A  R   +    G  GEV ++D   +   LD G + ++ 
Sbjct: 204 HGDRSRLHDIGVRVDTGNFFAAKRRGVVDGVDFGATGEVKKIDVDRICERLDGGSVVLLR 263

Query: 249 SVAADESGQMYNINADTVAGELAAALGAEKLILLTD 269
           ++    SG++ N N   VA   A A+GA+KLI + D
Sbjct: 264 NLGHSSSGEVLNCNTYEVATACALAIGADKLICIMD 294

BLAST of MC08g_new0037 vs. TAIR 10
Match: AT2G22910.1 (N-acetyl-l-glutamate synthase 1 )

HSP 1 Score: 49.3 bits (116), Expect = 7.3e-06
Identity = 63/279 (22.58%), Postives = 118/279 (42.29%), Query Frame = 0

Query: 44  SSSSLSIRCSATQQLEPVVDSVTPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRS 103
           S +  + +C+     E   + V   QF V    E+ P++   R  T VV   G  +    
Sbjct: 51  SPALTAAKCNIFDYAETGENLVGDKQF-VRWFREAWPYLWAHRSCTFVVTISGDVLDGPY 110

Query: 104 LQASVVNDLVLLSCVGLRPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDADTMK----- 163
               V+ D+  L  +G++ +LV G   +I+  L     E  +    RVTD+ +++     
Sbjct: 111 CDL-VLKDIAFLHHLGIKFVLVPGTQVQIDQLLAERGREPTYVGRYRVTDSASLQAAKEA 170

Query: 164 --IVSMVLVGDVNKN-LVSLINKAGASA----AGLCGVDGRLLTA--RPAPNAAQLGFVG 223
              +S+++   ++    +  I + G S+     G+    G    A  R   +    G  G
Sbjct: 171 AGAISVMIEAKLSPGPSIYNIRRHGDSSRLHETGVRVDTGNFFAAKRRGVVDGVDFGATG 230

Query: 224 EVARVDPTVLQALLDKGHIPVVSSVAADESGQMYNINADTVAGELAAALGAEKLILLTDV 283
            V ++D   ++  LD G + ++ ++    +G++ N N   VA   A A+GA+KLI + D 
Sbjct: 231 LVKKIDVDRIRERLDSGSVVLLRNLGHSSTGEVLNCNTYEVATACALAIGADKLICIMDG 290

Query: 284 AGILEDRDDPKSLVKQIDIKGVKKMMEEGRIGGGMIPKV 309
             + E+    + L  Q     V+K  ++  I    +  V
Sbjct: 291 PVLDENGHLVRFLTLQEADTLVRKRAQQSEIAANYVKAV 327

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SCL71.5e-12068.38Acetylglutamate kinase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NAGK PE... [more]
Q8YXG85.9e-10166.67Acetylglutamate kinase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) O... [more]
Q3MFQ81.3e-10066.67Acetylglutamate kinase OS=Trichormus variabilis (strain ATCC 29413 / PCC 7937) O... [more]
B0CAM33.8e-10066.19Acetylglutamate kinase OS=Acaryochloris marina (strain MBIC 11017) OX=329726 GN=... [more]
B0JHB18.5e-10063.90Acetylglutamate kinase OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=... [more]
Match NameE-valueIdentityDescription
XP_022131382.15.42e-237100.00acetylglutamate kinase, chloroplastic [Momordica charantia][more]
XP_038886528.14.87e-22494.27acetylglutamate kinase, chloroplastic [Benincasa hispida][more]
XP_022929565.12.22e-22294.84acetylglutamate kinase, chloroplastic-like [Cucurbita moschata] >KAG6598822.1 Ac... [more]
XP_022932833.16.37e-22294.56acetylglutamate kinase, chloroplastic-like [Cucurbita moschata][more]
XP_004138756.11.01e-22193.73acetylglutamate kinase, chloroplastic [Cucumis sativus] >KGN62888.1 hypothetical... [more]
Match NameE-valueIdentityDescription
A0A6J1BQV12.63e-237100.00acetylglutamate kinase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11100... [more]
A0A6J1EUQ81.08e-22294.84acetylglutamate kinase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1F2V33.08e-22294.56acetylglutamate kinase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A0A0LM564.90e-22293.73AA_kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G379190 P... [more]
A0A6J1K5098.84e-22294.56acetylglutamate kinase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
Match NameE-valueIdentityDescription
AT3G57560.11.1e-12168.38N-acetyl-l-glutamate kinase [more]
AT4G37670.21.3e-0725.36N-acetyl-l-glutamate synthase 2 [more]
AT4G37670.11.3e-0725.36N-acetyl-l-glutamate synthase 2 [more]
AT2G22910.17.3e-0622.58N-acetyl-l-glutamate synthase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001057Glutamate/acetylglutamate kinasePRINTSPR00474GLU5KINASEcoord: 246..273
score: 36.51
coord: 299..319
score: 29.28
coord: 119..133
score: 29.63
IPR036393Acetylglutamate kinase-like superfamilyGENE3D3.40.1160.10coord: 65..349
e-value: 4.8E-104
score: 349.4
IPR036393Acetylglutamate kinase-like superfamilySUPERFAMILY53633Carbamate kinase-likecoord: 71..348
IPR001048Aspartate/glutamate/uridylate kinasePFAMPF00696AA_kinasecoord: 88..325
e-value: 5.7E-43
score: 147.2
IPR004662Acetylglutamate kinase familyTIGRFAMTIGR00761TIGR00761coord: 89..324
e-value: 5.7E-84
score: 279.2
NoneNo IPR availablePIRSRPIRSR016496-1PIRSR016496-1coord: 209..348
e-value: 1.1E-11
score: 42.0
NoneNo IPR availablePANTHERPTHR23342N-ACETYLGLUTAMATE SYNTHASEcoord: 17..349
NoneNo IPR availablePANTHERPTHR23342:SF16BNAC04G55460D PROTEINcoord: 17..349
IPR037528Acetylglutamate kinase ArgBHAMAPMF_00082ArgBcoord: 87..342
score: 48.429764
IPR041727N-Acetyl-L-glutamate kinase, cyclicCDDcd04250AAK_NAGK-Ccoord: 74..347
e-value: 9.95194E-148
score: 416.136

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g_new0037.1MC08g_new0037.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006526 arginine biosynthetic process
biological_process GO:0016310 phosphorylation
cellular_component GO:0005759 mitochondrial matrix
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004042 acetyl-CoA:L-glutamate N-acetyltransferase activity
molecular_function GO:0003991 acetylglutamate kinase activity
molecular_function GO:0034618 arginine binding
molecular_function GO:0005524 ATP binding