MC08g_new0030 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g_new0030 vs. ExPASy Swiss-Prot
Match: Q9M817 (Protein NRT1/ PTR FAMILY 1.2 OS=Arabidopsis thaliana OX=3702 GN=NPF1.2 PE=1 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 1.8e-147
Identity = 263/545 (48.26%), Postives = 385/545 (70.64%), Query Frame = 0

Query: 29  GGLRTMPFIIANEVFEKISSLGLSPNMMFYFRDEYGFQIAAASSVLSLWSAASNALSIAG 88
           GG+ TMPFIIANE FEK++S GL PNM+ Y   +Y F +A  ++VL +WSAASN   + G
Sbjct: 23  GGIITMPFIIANEAFEKVASYGLLPNMIMYLIRDYRFGVAKGTNVLFMWSAASNFTPLLG 82

Query: 89  AVLADSYMGRFRVIILGSFCSFLGMALLWLTATIPQLKPSSC--SSSGILCDSANLYQLA 148
           A L+DSY+GRF  I + S  SFLGM LLWLTA +PQ+KPS C  +++G  C S+   QLA
Sbjct: 83  AFLSDSYLGRFLTISIASLSSFLGMVLLWLTAMLPQVKPSPCDPTAAGSHCGSSTASQLA 142

Query: 149 VLFTSLGFISIGAGCIKPCSMAFGADQLTNEKNSSNKGVLDTYFNWYYAASGISTVIGMT 208
           +L+++   ISIG+G I+PCS+AFGADQL N++N  N+ VL+++F WYYA+S ++ +I  T
Sbjct: 143 LLYSAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSAVAVLIAFT 202

Query: 209 VIVYIQDEFGWGTGYVVPAVLMLLSALIFLAGSSLYVKVKPAQSLFTGIFQVIVVAFKNR 268
            IVYIQ+  GW  G+ VPAVLML++AL+F+  S LYV     +SLFTG+ Q IV A+K R
Sbjct: 203 GIVYIQEHLGWKIGFGVPAVLMLIAALLFILASPLYVTRGVTKSLFTGLAQAIVAAYKKR 262

Query: 269 RLSLP--YSNFDQYYLGHDPKLLVPTDSMRCLNKACLIKDAENDLNPDGSVSNPWNLCSV 328
           +LSLP  + +FD YY   D ++  P+  +R LNKACLI + E ++  DG   NPW LC+ 
Sbjct: 263 KLSLPDHHDSFDCYYHMKDSEIKAPSQKLRFLNKACLISNREEEIGSDGFALNPWRLCTT 322

Query: 329 DQVESLKNFIRIIPVWSTGIIMVVCINQNALGALQAKIMNRHIT---GSFEFPAGSINVF 388
           D+VE LK  I++IP+WSTGI+M +  +Q++   LQA  M+R ++    SF+ PAGS  +F
Sbjct: 323 DKVEELKALIKVIPIWSTGIMMSINTSQSSFQLLQATSMDRRLSRHGSSFQVPAGSFGMF 382

Query: 389 MIISLSLWLVFYDRIIMPLLKRC-GHAGRLSPELRIGIGLLLSCFAMAIAAVVEATRRRI 448
            II+L+LW++ YDR ++PL  +  G   RLS +LR+G+GL +S  AMAI+A+VE+ RR+ 
Sbjct: 383 TIIALALWVILYDRAVIPLASKIRGRPFRLSVKLRMGLGLFMSFLAMAISAMVESFRRKK 442

Query: 449 AIEEGLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQIEYFFSKISKVSSSMAVALHT 508
           AI +G  +  NAVVD+SA+WLVPQ V+ G+AE   A+GQ E+F+++  K  SS+A +L  
Sbjct: 443 AISQGYANNSNAVVDISAMWLVPQYVLHGLAEALTAIGQTEFFYTEFPKSMSSIAASLFG 502

Query: 509 VEMSVANLVGSILVQIVNGITGKGNETSWLDNNLNKGHLDYFYWLCAALALVNLCGYLLY 566
           + M+VA+L+ S+++  VN +T +  + SW+ +N+NKGH +Y+YW+ A ++ +N+  Y++ 
Sbjct: 503 LGMAVASLLASVVLNAVNELTSRNGKESWVSDNINKGHYNYYYWVLAIMSFINVIYYVIC 562

BLAST of MC08g_new0030 vs. ExPASy Swiss-Prot
Match: Q8LPL2 (Protein NRT1/ PTR FAMILY 1.1 OS=Arabidopsis thaliana OX=3702 GN=NPF1.1 PE=1 SV=2)

HSP 1 Score: 507.3 bits (1305), Expect = 2.3e-142
Identity = 255/543 (46.96%), Postives = 374/543 (68.88%), Query Frame = 0

Query: 29  GGLRTMPFIIANEVFEKISSLGLSPNMMFYFRDEYGFQIAAASSVLSLWSAASNALSIAG 88
           GGL TMPFIIANE FEK++S GL  NM+ Y   +Y   +    +VL +W AA+N + + G
Sbjct: 25  GGLLTMPFIIANEGFEKVASYGLLQNMILYLMSDYRLGLVKGQTVLFMWVAATNFMPLVG 84

Query: 89  AVLADSYMGRFRVIILGSFCSFLGMALLWLTATIPQLKPSSC-SSSGILCDSANLYQLAV 148
           A L+DSY+GRF  I++ S  S LGM +LWLTA +PQ+KPS C +++G  C SA   QLA+
Sbjct: 85  AFLSDSYLGRFLTIVIASLSSLLGMVVLWLTAMLPQVKPSPCVATAGTNCSSATSSQLAL 144

Query: 149 LFTSLGFISIGAGCIKPCSMAFGADQLTNEKNSSNKGVLDTYFNWYYAASGISTVIGMTV 208
           L+T+   ISIG+G I+PCS+AFGADQL N++N  N+ VL+++F WYYA+S ++ +I  TV
Sbjct: 145 LYTAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSSVAVLIAFTV 204

Query: 209 IVYIQDEFGWGTGYVVPAVLMLLSALIFLAGSSLYVKVKPAQSLFTGIFQVIVVAFKNRR 268
           IVYIQD  GW  G+ +PA+LMLL+  +F+  S LYVK   ++SLFTG+ QV+  A+  R 
Sbjct: 205 IVYIQDHLGWKIGFGIPAILMLLAGFLFVFASPLYVKRDVSKSLFTGLAQVVAAAYVKRN 264

Query: 269 LSLP--YSNFDQYYLGHDPKLLVPTDSMRCLNKACLIKDAENDLNPDGSVSNPWNLCSVD 328
           L+LP  + + D YY   D +L  P+D +R LNKAC I + + DL  DG   N W LC+ D
Sbjct: 265 LTLPDHHDSRDCYYRLKDSELKAPSDKLRFLNKACAISNRDEDLGSDGLALNQWRLCTTD 324

Query: 329 QVESLKNFIRIIPVWSTGIIMVVCINQNALGALQAKIMNRHIT--GSFEFPAGSINVFMI 388
           QVE LK  +++IPVWSTGI+M + ++QN+   LQAK M+R ++   +F+ PAGS  +F I
Sbjct: 325 QVEKLKALVKVIPVWSTGIMMSINVSQNSFQLLQAKSMDRRLSSNSTFQIPAGSFGMFTI 384

Query: 389 ISLSLWLVFYDRIIMPLLKRC-GHAGRLSPELRIGIGLLLSCFAMAIAAVVEATRRRIAI 448
           I+L  W+V YDR I+PL  +  G   R++ ++R+G+GL +S  AMA++A VE  RR+ AI
Sbjct: 385 IALISWVVLYDRAILPLASKIRGRPVRVNVKIRMGLGLFISFLAMAVSATVEHYRRKTAI 444

Query: 449 EEGLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQIEYFFSKISKVSSSMAVALHTVE 508
            +GL +  N+ V +SA+WLVPQ V+ G+AE    +GQ E+F+++  K  SS+A +L  + 
Sbjct: 445 SQGLANDANSTVSISAMWLVPQYVLHGLAEALTGIGQTEFFYTEFPKSMSSIAASLFGLG 504

Query: 509 MSVANLVGSILVQIVNGITGKGNETSWLDNNLNKGHLDYFYWLCAALALVNLCGYLLYNR 566
           M+VAN++ S+++  V   + +GN  SW+++N+NKGH DY+YW+ A L+ VN+  Y++ + 
Sbjct: 505 MAVANILASVILNAVKNSSKQGN-VSWIEDNINKGHYDYYYWVLAILSFVNVIYYVVCSW 564

BLAST of MC08g_new0030 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 2.4e-107
Identity = 217/566 (38.34%), Postives = 332/566 (58.66%), Query Frame = 0

Query: 27  RTGGLRTMPFIIANEVFEKISSLGLSPNMMFYFRDEYGFQIAAASSVLSLWSAASNALSI 86
           + GG R + FI+ NE  E++ S+GL  N M Y    +  +   A++V+++WS  +N   +
Sbjct: 51  KPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPL 110

Query: 87  AGAVLADSYMGRFRVIILGSFCSFLGMALLWLTATIPQLKPSSCSSSGIL-CDSANLYQL 146
            GA ++D+Y+GRF+ I   SF + LG+  + LTA+ PQL P+SC+S   L C   N  Q+
Sbjct: 111 VGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQI 170

Query: 147 AVLFTSLGFISIGAGCIKPCSMAFGADQLTNEKNSSNKGVLDTYFNWYYAASGISTVIGM 206
            VL   L F+S+G+G I+PCS+ FG DQ         KGV  ++FNWYY    +  +I  
Sbjct: 171 GVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVA-SFFNWYYMTFTVVLIITQ 230

Query: 207 TVIVYIQDEFGWGTGYVVPAVLMLLSALIFLAGSSLYVKVKPAQSLFTGIFQVIVVAFKN 266
           TV+VYIQD+  W  G+ +P  LM L+ ++F AG   YV VKP  S+F+GI QVIV A K 
Sbjct: 231 TVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKK 290

Query: 267 RRLSLPYSNFDQYYLGHDPKL-------LVPTDSMRCLNKACLIKDAENDLNPDGSVSNP 326
           R+L LP  + D     +DP +       L  ++  RCL+KA ++   E DL P+G  ++ 
Sbjct: 291 RKLKLPAED-DGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVV--IEGDLTPEGPPADK 350

Query: 327 WNLCSVDQVESLKNFIRIIPVWSTGIIMVVCI-NQNALGALQAKIMNRHITGSFEFPAGS 386
           W LCSV +VE +K  IRI+P+WS GII +  +  Q      QA  M+R++   FE PAGS
Sbjct: 351 WRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGS 410

Query: 387 INVFMIISLSLWLVFYDRIIMPLLKR-CGHAGRLSPELRIGIGLLLSCFAMAIAAVVEAT 446
           ++V  ++++ ++L FYDR+ +P ++R  GH   ++   RIG G++ + F+M +A +VE  
Sbjct: 411 LSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERM 470

Query: 447 RRRIAIEEGLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQIEYFFSKISKVSSSMAV 506
           RR  +I  G    P  +  MS  WL PQ +++G+ E FN +GQIE+F S+  +   S+A 
Sbjct: 471 RRIRSINAG---DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIAN 530

Query: 507 ALHTVEMSVANLVGSILVQIVNGITGKGNETSWLDNNLNKGHLDYFYWLCAALALVNLCG 566
           +L ++  + ++ + S LV +V+  +G  +   WL+ NLN G LDYFY+L A L +VNL  
Sbjct: 531 SLFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGVVNLVY 590

Query: 567 YLLYNRAYGFSEKIQGNESSEIQDFE 583
           +    R Y +   +       I+DFE
Sbjct: 591 FWYCARGYRYKVGL------PIEDFE 603

BLAST of MC08g_new0030 vs. ExPASy Swiss-Prot
Match: Q9SX20 (Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 5.5e-99
Identity = 217/569 (38.14%), Postives = 336/569 (59.05%), Query Frame = 0

Query: 21  HQASNCRTGGLRTMPFIIANEVFEKISSLGLSPNMMFYFRDEYGFQIAAASSVLSLWSAA 80
           H   N   GGL TMPFI ANE+ EK++ +G   NM+ Y   +    +  A++ L+ ++  
Sbjct: 17  HGRPNRPKGGLITMPFIFANEICEKLAVVGFHANMISYLTTQLHLPLTKAANTLTNFAGT 76

Query: 81  SNALSIAGAVLADSYMGRFRVIILGSFCSFLGMALLWLTATIPQLKPSSCSSSGILCDSA 140
           S+   + GA +ADS+ GRF  I   S    +GM LL ++A IP L+P  C    + C  A
Sbjct: 77  SSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPTLRPPPCKGEEV-CVVA 136

Query: 141 NLYQLAVLFTSLGFISIGAGCIKPCSMAFGADQLTNEKNSSNKGVLDTYFNWYYAASGIS 200
           +  QL++L+ +L   ++G+G I+PC +AFGADQ  +E + +       YFNWYY   G +
Sbjct: 137 DTAQLSILYVALLLGALGSGGIRPCVVAFGADQF-DESDPNQTTKTWNYFNWYYFCMGAA 196

Query: 201 TVIGMTVIVYIQDEFGWGTGYVVPAVLMLLSALIFLAGSSLYVKVKPAQSLFTGIFQVIV 260
            ++ +TV+V+IQD  GWG G  +P V M LS + F+ G  LY  + PA S FT + QV V
Sbjct: 197 VLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRHLVPAGSPFTRLIQVGV 256

Query: 261 VAFKNRRL------SLPYSNFD---QYYLGHDPKLLVPTDSMRCLNKACLIKDAENDLNP 320
            AF+ R+L      SL Y N +      LG     L  T  M  L+KA ++ + E++L P
Sbjct: 257 AAFRKRKLRMVSDPSLLYFNDEIDAPISLGGK---LTHTKHMSFLDKAAIVTE-EDNLKP 316

Query: 321 DGSVSNPWNLCSVDQVESLKNFIRIIPVWSTGIIMVVCINQNALGAL-QAKIMNRHITGS 380
            G + N W L +V +VE LK+ IR+ P+ ++GI+++    Q    +L QAK MNRH+T S
Sbjct: 317 -GQIPNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQGTFSLQQAKTMNRHLTNS 376

Query: 381 FEFPAGSINVFMIISLSLWLVFYDRIIMPLLKR-CGHAGRLSPELRIGIGLLLSCFAMAI 440
           F+ PAGS++VF  +++   ++FYDR+ + + ++  G    ++   R+GIG ++S  A  +
Sbjct: 377 FQIPAGSMSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITFLHRMGIGFVISIIATLV 436

Query: 441 AAVVEATRRRIAIEEGLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQIEYFFSKISK 500
           A  VE  R+ +AIE GL D+P+ +V +S LWL+PQ  + GVAE F ++G +E+F+ +  +
Sbjct: 437 AGFVEVKRKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFMSIGHLEFFYDQAPE 496

Query: 501 VSSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNETSWL-DNNLNKGHLDYFYWLCAA 560
              S A AL  + +S+ N V ++LV +V+  + K + ++WL DNNLN+G L+YFYWL   
Sbjct: 497 SMRSTATALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNLNRGRLEYFYWLITV 556

Query: 561 LALVNLCGYLLYNRAYGFSEKIQGNESSE 578
           L  VNL  YL   + Y + + +Q + S E
Sbjct: 557 LQAVNLVYYLWCAKIYTY-KPVQVHHSKE 577

BLAST of MC08g_new0030 vs. ExPASy Swiss-Prot
Match: Q9M9V7 (Protein NRT1/ PTR FAMILY 2.9 OS=Arabidopsis thaliana OX=3702 GN=NPF2.9 PE=1 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 1.4e-94
Identity = 196/563 (34.81%), Postives = 324/563 (57.55%), Query Frame = 0

Query: 30  GLRTMPFIIANEVFEKISSLGLSPNMMFYFRDEYGFQIAAASSVLSLWSAASNALSIAGA 89
           G + MPFII NE FEK+  +G S N++ Y    +  +   A+ V++++   SN  +I  A
Sbjct: 22  GWKVMPFIIGNETFEKLGIVGSSSNLVIYLTTVFNMKSITAAKVVNIYGGTSNFGTIVAA 81

Query: 90  VLADSYMGRFRVIILGSFCSFLGMALLWLTATIPQLKPSSCSSS-GILCDSANLYQLAVL 149
            L DSY GR++ +       FLG   + LTA I  L P+ C+   G +C+  ++ Q+  L
Sbjct: 82  FLCDSYFGRYKTLSFAMIACFLGSVAMDLTAVIHPLHPAQCAKEIGSVCNGPSIGQIMFL 141

Query: 150 FTSLGFISIGAGCIKPCSMAFGADQLTNEKNSSNKGVLDTYFNWYYAASGISTVIGMTVI 209
             ++  + IGAG I+PC++ FGADQ   +     +G+ +++FNWY+     + ++ +T+I
Sbjct: 142 AGAMVLLVIGAGGIRPCNLPFGADQFDPKTKEGKRGI-ESFFNWYFFTFTFAQMVSLTLI 201

Query: 210 VYIQDEFGWGTGYVVPAVLMLLSALIFLAGSSLYVKVKPAQSLFTGIFQVIVVAFKNRRL 269
           VY+Q    W  G  +PA+LMLL  +IF AGS LYVKVK + S    I +VIVVA K RRL
Sbjct: 202 VYVQSNVSWSIGLAIPAILMLLGCIIFFAGSKLYVKVKASGSPIHSITRVIVVAIKKRRL 261

Query: 270 SLPYSNFDQYYLGHDPK--LLVPTDSMRCLNKACLIKDAENDLNPDGSVSNPWNLCSVDQ 329
                N    Y+  D K   L  T+  R L+K+  I+  ++ LN DGS  + W LCS+ Q
Sbjct: 262 KPVGPNELYNYIASDFKNSKLGHTEQFRFLDKSA-IQTQDDKLNKDGSPVDAWKLCSMQQ 321

Query: 330 VESLKNFIRIIPVW-STGIIMVVCINQNALGALQAKIMNRHI-TGSFEFPAGSINVFMII 389
           VE +K  IR++PVW S  +  +  I Q      Q+   +R +  GSF+ PAGS  VF+++
Sbjct: 322 VEEVKCVIRVLPVWLSAALFYLAYIQQTTYTIFQSLQSDRRLGPGSFQIPAGSYTVFLML 381

Query: 390 SLSLWLVFYDRIIMPLLKR-CGHAGRLSPELRIGIGLLLSCFAMAIAAVVEATRRRIAIE 449
            +++++  YDR+++P L++  G  G ++   R+G GL L   +M ++A+VE  RR++A+ 
Sbjct: 382 GMTIFIPIYDRVLVPFLRKYTGRDGGITQLQRVGAGLFLCITSMMVSAIVEQYRRKVALT 441

Query: 450 E---GLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQIEYFFSKISKVSSSMAVALHT 509
           +   GL  +  A+  MS +WL+PQ V++G+A+    VGQ+E+++ +  +   S A +L+ 
Sbjct: 442 KPTLGLAPRKGAISSMSGMWLIPQLVLMGIADALAGVGQMEFYYKQFPENMRSFAGSLYY 501

Query: 510 VEMSVANLVGSILVQIVNGITGKGNETSWLDNNLNKGHLDYFYWLCAALALVNLCGYLLY 569
             + +A+ + + L+  V+  T   +  SWL  +LNKG L+YFY+L A +  +NL  +LL 
Sbjct: 502 CGIGLASYLSTFLLSAVHDTTEGFSGGSWLPEDLNKGRLEYFYFLVAGMMTLNLAYFLLV 561

Query: 570 NRAYGFSEKIQGNESSEIQDFEY 584
           +  Y + + +  ++  +    E+
Sbjct: 562 SHWYRYKDVVAKDKDMDKTSAEF 582

BLAST of MC08g_new0030 vs. NCBI nr
Match: XP_022131895.1 (protein NRT1/ PTR FAMILY 1.2-like [Momordica charantia])

HSP 1 Score: 1156 bits (2990), Expect = 0.0
Identity = 589/589 (100.00%), Postives = 589/589 (100.00%), Query Frame = 0

Query: 1   MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIANEVFEKISSLGLSPNMMFYFR 60
           MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIANEVFEKISSLGLSPNMMFYFR
Sbjct: 1   MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIANEVFEKISSLGLSPNMMFYFR 60

Query: 61  DEYGFQIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIILGSFCSFLGMALLWLTA 120
           DEYGFQIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIILGSFCSFLGMALLWLTA
Sbjct: 61  DEYGFQIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIILGSFCSFLGMALLWLTA 120

Query: 121 TIPQLKPSSCSSSGILCDSANLYQLAVLFTSLGFISIGAGCIKPCSMAFGADQLTNEKNS 180
           TIPQLKPSSCSSSGILCDSANLYQLAVLFTSLGFISIGAGCIKPCSMAFGADQLTNEKNS
Sbjct: 121 TIPQLKPSSCSSSGILCDSANLYQLAVLFTSLGFISIGAGCIKPCSMAFGADQLTNEKNS 180

Query: 181 SNKGVLDTYFNWYYAASGISTVIGMTVIVYIQDEFGWGTGYVVPAVLMLLSALIFLAGSS 240
           SNKGVLDTYFNWYYAASGISTVIGMTVIVYIQDEFGWGTGYVVPAVLMLLSALIFLAGSS
Sbjct: 181 SNKGVLDTYFNWYYAASGISTVIGMTVIVYIQDEFGWGTGYVVPAVLMLLSALIFLAGSS 240

Query: 241 LYVKVKPAQSLFTGIFQVIVVAFKNRRLSLPYSNFDQYYLGHDPKLLVPTDSMRCLNKAC 300
           LYVKVKPAQSLFTGIFQVIVVAFKNRRLSLPYSNFDQYYLGHDPKLLVPTDSMRCLNKAC
Sbjct: 241 LYVKVKPAQSLFTGIFQVIVVAFKNRRLSLPYSNFDQYYLGHDPKLLVPTDSMRCLNKAC 300

Query: 301 LIKDAENDLNPDGSVSNPWNLCSVDQVESLKNFIRIIPVWSTGIIMVVCINQNALGALQA 360
           LIKDAENDLNPDGSVSNPWNLCSVDQVESLKNFIRIIPVWSTGIIMVVCINQNALGALQA
Sbjct: 301 LIKDAENDLNPDGSVSNPWNLCSVDQVESLKNFIRIIPVWSTGIIMVVCINQNALGALQA 360

Query: 361 KIMNRHITGSFEFPAGSINVFMIISLSLWLVFYDRIIMPLLKRCGHAGRLSPELRIGIGL 420
           KIMNRHITGSFEFPAGSINVFMIISLSLWLVFYDRIIMPLLKRCGHAGRLSPELRIGIGL
Sbjct: 361 KIMNRHITGSFEFPAGSINVFMIISLSLWLVFYDRIIMPLLKRCGHAGRLSPELRIGIGL 420

Query: 421 LLSCFAMAIAAVVEATRRRIAIEEGLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQI 480
           LLSCFAMAIAAVVEATRRRIAIEEGLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQI
Sbjct: 421 LLSCFAMAIAAVVEATRRRIAIEEGLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQI 480

Query: 481 EYFFSKISKVSSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNETSWLDNNLNKGHLD 540
           EYFFSKISKVSSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNETSWLDNNLNKGHLD
Sbjct: 481 EYFFSKISKVSSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNETSWLDNNLNKGHLD 540

Query: 541 YFYWLCAALALVNLCGYLLYNRAYGFSEKIQGNESSEIQDFEYRNLPSS 589
           YFYWLCAALALVNLCGYLLYNRAYGFSEKIQGNESSEIQDFEYRNLPSS
Sbjct: 541 YFYWLCAALALVNLCGYLLYNRAYGFSEKIQGNESSEIQDFEYRNLPSS 589

BLAST of MC08g_new0030 vs. NCBI nr
Match: XP_022132097.1 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 [Momordica charantia])

HSP 1 Score: 988 bits (2554), Expect = 0.0
Identity = 490/589 (83.19%), Postives = 540/589 (91.68%), Query Frame = 0

Query: 1   MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIANEVFEKISSLGLSPNMMFYFR 60
           MEVVEN EKTGIA+SS EGTHQASNCRTGGLRTMPFII NE FEKI+SLGL+PNM+FY R
Sbjct: 1   MEVVENEEKTGIAQSSSEGTHQASNCRTGGLRTMPFIIVNETFEKIASLGLTPNMVFYLR 60

Query: 61  DEYGFQIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIILGSFCSFLGMALLWLTA 120
           D+YGFQIAAASS+LSLWSAASNAL+IAGAVLADSY+GRFR IILGSF SFLGMALLWLTA
Sbjct: 61  DDYGFQIAAASSILSLWSAASNALAIAGAVLADSYVGRFRSIILGSFSSFLGMALLWLTA 120

Query: 121 TIPQLKPSSCSSSGILCDSANLYQLAVLFTSLGFISIGAGCIKPCSMAFGADQLTNEKNS 180
            IPQLKPSSCSSSGILCDSAN YQLA+LFTSLGFISIGAGCI+PCS+AFGADQLTNEK  
Sbjct: 121 MIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKP 180

Query: 181 SNKGVLDTYFNWYYAASGISTVIGMTVIVYIQDEFGWGTGYVVPAVLMLLSALIFLAGSS 240
           +N+GVLDTYFNWYYA+SGI++VI +T+IVYIQDEFGW TG+ VPAVLML S LIFL GSS
Sbjct: 181 NNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKVKPAQSLFTGIFQVIVVAFKNRRLSLPYSNFDQYYLGHDPKLLVPTDSMRCLNKAC 300
           LYVKVKP QSL TG+ QVIVVAFKNRRLSLPYSN+DQYYLG DPK LVPTDSMRCLNKAC
Sbjct: 241 LYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTDSMRCLNKAC 300

Query: 301 LIKDAENDLNPDGSVSNPWNLCSVDQVESLKNFIRIIPVWSTGIIMVVCINQNALGALQA 360
           LIKDAENDLNPDGS+SNPWNLCSV+ VESLKNF+RIIP+WSTGI+M+VC+NQN+ G LQA
Sbjct: 301 LIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLNQNSFGTLQA 360

Query: 361 KIMNRHITGSFEFPAGSINVFMIISLSLWLVFYDRIIMPLLKRCGHAGRLSPELRIGIGL 420
           K MNRHITGSFE PAGSI V MIISLS+WL FYDRI++PLL+RCGHAG LSP LR+GIGL
Sbjct: 361 KTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLSPRLRMGIGL 420

Query: 421 LLSCFAMAIAAVVEATRRRIAIEEGLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQI 480
           LLSCFAMAI+AVVE TRRRIA EEGLEDQP AV+DMS LW+VPQCVVLGVAEGFNA+GQI
Sbjct: 421 LLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQI 480

Query: 481 EYFFSKISKVSSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNETSWLDNNLNKGHLD 540
           EYF+S++ K  SS+AVAL+TVEM+VA+LVGS LVQ+VNGITGKGNETSWLDNNLNKGHLD
Sbjct: 481 EYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLD 540

Query: 541 YFYWLCAALALVNLCGYLLYNRAYGFSEKIQGNESSEIQDFEYRNLPSS 589
           YFYWLCAALAL+N  GYLL NR YG SE  + NES+E+QDFEYR LPSS
Sbjct: 541 YFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFEYRKLPSS 589

BLAST of MC08g_new0030 vs. NCBI nr
Match: XP_022962308.1 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 867 bits (2241), Expect = 2.18e-312
Identity = 430/589 (73.01%), Postives = 509/589 (86.42%), Query Frame = 0

Query: 1   MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIANEVFEKISSLGLSPNMMFYFR 60
           MEV+E+ EKTGIA S  E   QASN   GGL+TMPFII NE FE+++SLGL+PNM+FY R
Sbjct: 1   MEVLEDGEKTGIAASGSEEAEQASNYTNGGLKTMPFIIVNETFERVASLGLTPNMVFYLR 60

Query: 61  DEYGFQIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIILGSFCSFLGMALLWLTA 120
           D +GF+IAAAS +LSLWSAASNAL+I GAVLADSY+GRF VIILGS  S LGM LLWLTA
Sbjct: 61  DVFGFEIAAASIILSLWSAASNALAIVGAVLADSYLGRFCVIILGSCSSLLGMILLWLTA 120

Query: 121 TIPQLKPSSCSSSGILCDSANLYQLAVLFTSLGFISIGAGCIKPCSMAFGADQLTNEKNS 180
            IPQL+P+SCS SGILCDSAN YQLAVLF+SL FISIGAGCI+PCS+AFGADQLT E+  
Sbjct: 121 IIPQLRPASCSPSGILCDSANPYQLAVLFSSLVFISIGAGCIRPCSVAFGADQLTKEEKP 180

Query: 181 SNKGVLDTYFNWYYAASGISTVIGMTVIVYIQDEFGWGTGYVVPAVLMLLSALIFLAGSS 240
           +N+ VLD+YFNWYYA+ GI+T+I +++IV+IQD+FGWG G+ VPAVLML S LIFL GSS
Sbjct: 181 NNESVLDSYFNWYYASIGIATIIALSLIVFIQDKFGWGIGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKVKPAQSLFTGIFQVIVVAFKNRRLSLPYSNFDQYYLGHDPKLLVPTDSMRCLNKAC 300
           LYVKVKP QSL TG  +VIVVAFKNR+LSLP+SNFDQYYLG DPK  +PT+SMR LNKAC
Sbjct: 241 LYVKVKPTQSLLTGFLRVIVVAFKNRKLSLPFSNFDQYYLGRDPKCRIPTNSMRYLNKAC 300

Query: 301 LIKDAENDLNPDGSVSNPWNLCSVDQVESLKNFIRIIPVWSTGIIMVVCINQNALGALQA 360
           LIKDA+N+LNPDGSVSNPWNLCSVDQVESLK F+RIIP+WSTGI+M+VC+NQN+ G +QA
Sbjct: 301 LIKDAKNNLNPDGSVSNPWNLCSVDQVESLKAFLRIIPMWSTGILMLVCLNQNSFGTIQA 360

Query: 361 KIMNRHITGSFEFPAGSINVFMIISLSLWLVFYDRIIMPLLKRCGHAGRLSPELRIGIGL 420
           K MNRHIT SFE PAGSI +FM++SL  W++FYDRI++PLL R GHAG LSP+LRIGIGL
Sbjct: 361 KTMNRHITRSFEIPAGSITIFMVLSLFSWIMFYDRILVPLLARHGHAGGLSPKLRIGIGL 420

Query: 421 LLSCFAMAIAAVVEATRRRIAIEEGLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQI 480
           LLSCFAMAI+A V   RRR+AIE+G EDQPNAV++MSALW+VPQCVVLGVAE  N+VGQ+
Sbjct: 421 LLSCFAMAISAGVACIRRRVAIEQGFEDQPNAVINMSALWMVPQCVVLGVAEALNSVGQV 480

Query: 481 EYFFSKISKVSSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNETSWLDNNLNKGHLD 540
           EYF+S++ K  SS+AVAL++ EM+VANLVGSI+VQ+VN ITG+GN+TSWLDNN+NKGHLD
Sbjct: 481 EYFYSQMPKTLSSVAVALYSFEMAVANLVGSIIVQMVNSITGEGNKTSWLDNNVNKGHLD 540

Query: 541 YFYWLCAALALVNLCGYLLYNRAYGFSEKIQGNESSEIQDFEYRNLPSS 589
           YFYWLCAALAL+N  GYL+Y  A G  +K    E +E++DFEYRNLPSS
Sbjct: 541 YFYWLCAALALINFFGYLVYCWALGRQDK----EPNEMEDFEYRNLPSS 585

BLAST of MC08g_new0030 vs. NCBI nr
Match: XP_022996584.1 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 864 bits (2232), Expect = 3.93e-311
Identity = 429/589 (72.84%), Postives = 509/589 (86.42%), Query Frame = 0

Query: 1   MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIANEVFEKISSLGLSPNMMFYFR 60
           MEV+E+ EKTGI+ S  EGT QASN   GGL+TMPFII NE FE+++SLGL+PNM+FY R
Sbjct: 1   MEVLEDGEKTGISASGSEGTEQASNHTNGGLKTMPFIIVNETFERVASLGLTPNMVFYLR 60

Query: 61  DEYGFQIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIILGSFCSFLGMALLWLTA 120
           D +GFQIA AS +LSLWSAASNAL+I GAVLADSY+GRF VIILGS  S LGM LLWLTA
Sbjct: 61  DVFGFQIADASIILSLWSAASNALAIVGAVLADSYLGRFCVIILGSCSSLLGMILLWLTA 120

Query: 121 TIPQLKPSSCSSSGILCDSANLYQLAVLFTSLGFISIGAGCIKPCSMAFGADQLTNEKNS 180
            IPQL+P+SCS SGILCDSAN YQLAVLF+SL FISIGAGCI+PCS+AFGADQLT E+  
Sbjct: 121 IIPQLRPASCSPSGILCDSANPYQLAVLFSSLVFISIGAGCIRPCSVAFGADQLTKEEKP 180

Query: 181 SNKGVLDTYFNWYYAASGISTVIGMTVIVYIQDEFGWGTGYVVPAVLMLLSALIFLAGSS 240
           +N+ VLD+YFNWYYA+ GI+T+  +++IV+IQD+FGWG G+ VPAVLML S LIFL GSS
Sbjct: 181 NNESVLDSYFNWYYASIGIATIFALSLIVFIQDKFGWGIGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKVKPAQSLFTGIFQVIVVAFKNRRLSLPYSNFDQYYLGHDPKLLVPTDSMRCLNKAC 300
           LYVKVKP QSL TG  +VIVVAFKNR+LSLP+SNFDQYYLG DPK  +PT+SMR LNKAC
Sbjct: 241 LYVKVKPTQSLLTGFLRVIVVAFKNRKLSLPFSNFDQYYLGCDPKCPIPTNSMRYLNKAC 300

Query: 301 LIKDAENDLNPDGSVSNPWNLCSVDQVESLKNFIRIIPVWSTGIIMVVCINQNALGALQA 360
           LIKDA+N+LNPDGSVSNPWNLCSVDQVESLK F+RIIP+WSTGI+M+VC+NQN+ G +QA
Sbjct: 301 LIKDAKNNLNPDGSVSNPWNLCSVDQVESLKAFLRIIPMWSTGILMLVCLNQNSFGTIQA 360

Query: 361 KIMNRHITGSFEFPAGSINVFMIISLSLWLVFYDRIIMPLLKRCGHAGRLSPELRIGIGL 420
           K MNRHIT SFE PAGSI +FM++SL  W++FYDRI++PLL R GHAG LSP+LRIGIGL
Sbjct: 361 KTMNRHITRSFEIPAGSITIFMVLSLFSWIMFYDRILVPLLARYGHAGGLSPKLRIGIGL 420

Query: 421 LLSCFAMAIAAVVEATRRRIAIEEGLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQI 480
           LLSCFAMAI+A V   RRR+AIE+G EDQPNAV++MSALW+VPQCVVLGVAE  N+VGQ+
Sbjct: 421 LLSCFAMAISAGVACIRRRVAIEQGFEDQPNAVINMSALWMVPQCVVLGVAEALNSVGQV 480

Query: 481 EYFFSKISKVSSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNETSWLDNNLNKGHLD 540
           EYF+S++ K+ SS+AVAL++ EM+VANLVGSI+VQ+VN ITG+GN+TSWLDNN+NKGHLD
Sbjct: 481 EYFYSQMPKMLSSVAVALYSFEMAVANLVGSIIVQMVNSITGEGNKTSWLDNNVNKGHLD 540

Query: 541 YFYWLCAALALVNLCGYLLYNRAYGFSEKIQGNESSEIQDFEYRNLPSS 589
           YFYWLCAALAL+N  GYL+   A G  +K    E +E++DFEYRNLPSS
Sbjct: 541 YFYWLCAALALINFFGYLVCCWALGRQDK----EPNEMEDFEYRNLPSS 585

BLAST of MC08g_new0030 vs. NCBI nr
Match: KAG6598782.1 (Protein NRT1/ PTR FAMILY 1.2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 863 bits (2231), Expect = 5.58e-311
Identity = 428/589 (72.67%), Postives = 507/589 (86.08%), Query Frame = 0

Query: 1   MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIANEVFEKISSLGLSPNMMFYFR 60
           MEV+E+ EK+GIA S  EG  QASN   GGL+TMPFII NE FE+++SLGL+PNM+FY R
Sbjct: 1   MEVLEDGEKSGIAASGSEGAEQASNYTNGGLKTMPFIIVNETFERVASLGLTPNMVFYLR 60

Query: 61  DEYGFQIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIILGSFCSFLGMALLWLTA 120
           D +GF+IAAAS +LSLWSAASNAL+I GAVLADSY+GRF VIILGS  S LGM LLWLTA
Sbjct: 61  DVFGFEIAAASIILSLWSAASNALAIVGAVLADSYLGRFCVIILGSCSSLLGMILLWLTA 120

Query: 121 TIPQLKPSSCSSSGILCDSANLYQLAVLFTSLGFISIGAGCIKPCSMAFGADQLTNEKNS 180
            IPQL+P SCS SGILCDSAN YQLAVLF+SL FISIGAGCI+PCS+AFGADQLT E+  
Sbjct: 121 IIPQLRPESCSPSGILCDSANPYQLAVLFSSLVFISIGAGCIRPCSVAFGADQLTKEEKP 180

Query: 181 SNKGVLDTYFNWYYAASGISTVIGMTVIVYIQDEFGWGTGYVVPAVLMLLSALIFLAGSS 240
           +N+ VLD+YFNWYYA+ GI+T++ +++IV+IQD+FGWG G+ VPAVLML S LIFL GSS
Sbjct: 181 NNESVLDSYFNWYYASIGIATIVALSLIVFIQDKFGWGIGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKVKPAQSLFTGIFQVIVVAFKNRRLSLPYSNFDQYYLGHDPKLLVPTDSMRCLNKAC 300
           LYVKVKP QSL TG  +VIVVAFKNR+LSLP+SNFDQYYLG DPK  +PT+SMR LNKAC
Sbjct: 241 LYVKVKPTQSLLTGFLRVIVVAFKNRKLSLPFSNFDQYYLGRDPKCRIPTNSMRYLNKAC 300

Query: 301 LIKDAENDLNPDGSVSNPWNLCSVDQVESLKNFIRIIPVWSTGIIMVVCINQNALGALQA 360
           LIKDA+N+LNPDGSVSNPWNLCSVDQVESLK F+RIIP+WSTGI+M+VC+NQN+ G +QA
Sbjct: 301 LIKDAKNNLNPDGSVSNPWNLCSVDQVESLKAFLRIIPMWSTGILMLVCLNQNSFGTIQA 360

Query: 361 KIMNRHITGSFEFPAGSINVFMIISLSLWLVFYDRIIMPLLKRCGHAGRLSPELRIGIGL 420
           K MNRHIT SFE PAGSI +FM++SL  W++FYDRI++PLL R GHAG LSP+LRIGIGL
Sbjct: 361 KTMNRHITRSFEIPAGSITIFMVLSLFSWIMFYDRILVPLLARHGHAGGLSPKLRIGIGL 420

Query: 421 LLSCFAMAIAAVVEATRRRIAIEEGLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQI 480
           LLSCFAMAI+A V   RRR+AIE+G EDQPNAV++MSALW+VPQCVVLGVAE  N+VGQ+
Sbjct: 421 LLSCFAMAISAGVACIRRRVAIEQGFEDQPNAVINMSALWMVPQCVVLGVAEALNSVGQV 480

Query: 481 EYFFSKISKVSSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNETSWLDNNLNKGHLD 540
           EYF+S++ K  SS+AVAL++ EM VANLVGSI+VQ+VN ITG+GN+TSWLDNN+NKGHLD
Sbjct: 481 EYFYSQMPKTLSSVAVALYSFEMVVANLVGSIIVQMVNSITGEGNKTSWLDNNVNKGHLD 540

Query: 541 YFYWLCAALALVNLCGYLLYNRAYGFSEKIQGNESSEIQDFEYRNLPSS 589
           YFYWLCAALAL+N  GYL+   A G  +K    E +E++DFEYRNLPSS
Sbjct: 541 YFYWLCAALALINFFGYLVCCWALGRQDK----EPNEMEDFEYRNLPSS 585

BLAST of MC08g_new0030 vs. ExPASy TrEMBL
Match: A0A6J1BQY7 (protein NRT1/ PTR FAMILY 1.2-like OS=Momordica charantia OX=3673 GN=LOC111004925 PE=3 SV=1)

HSP 1 Score: 1156 bits (2990), Expect = 0.0
Identity = 589/589 (100.00%), Postives = 589/589 (100.00%), Query Frame = 0

Query: 1   MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIANEVFEKISSLGLSPNMMFYFR 60
           MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIANEVFEKISSLGLSPNMMFYFR
Sbjct: 1   MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIANEVFEKISSLGLSPNMMFYFR 60

Query: 61  DEYGFQIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIILGSFCSFLGMALLWLTA 120
           DEYGFQIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIILGSFCSFLGMALLWLTA
Sbjct: 61  DEYGFQIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIILGSFCSFLGMALLWLTA 120

Query: 121 TIPQLKPSSCSSSGILCDSANLYQLAVLFTSLGFISIGAGCIKPCSMAFGADQLTNEKNS 180
           TIPQLKPSSCSSSGILCDSANLYQLAVLFTSLGFISIGAGCIKPCSMAFGADQLTNEKNS
Sbjct: 121 TIPQLKPSSCSSSGILCDSANLYQLAVLFTSLGFISIGAGCIKPCSMAFGADQLTNEKNS 180

Query: 181 SNKGVLDTYFNWYYAASGISTVIGMTVIVYIQDEFGWGTGYVVPAVLMLLSALIFLAGSS 240
           SNKGVLDTYFNWYYAASGISTVIGMTVIVYIQDEFGWGTGYVVPAVLMLLSALIFLAGSS
Sbjct: 181 SNKGVLDTYFNWYYAASGISTVIGMTVIVYIQDEFGWGTGYVVPAVLMLLSALIFLAGSS 240

Query: 241 LYVKVKPAQSLFTGIFQVIVVAFKNRRLSLPYSNFDQYYLGHDPKLLVPTDSMRCLNKAC 300
           LYVKVKPAQSLFTGIFQVIVVAFKNRRLSLPYSNFDQYYLGHDPKLLVPTDSMRCLNKAC
Sbjct: 241 LYVKVKPAQSLFTGIFQVIVVAFKNRRLSLPYSNFDQYYLGHDPKLLVPTDSMRCLNKAC 300

Query: 301 LIKDAENDLNPDGSVSNPWNLCSVDQVESLKNFIRIIPVWSTGIIMVVCINQNALGALQA 360
           LIKDAENDLNPDGSVSNPWNLCSVDQVESLKNFIRIIPVWSTGIIMVVCINQNALGALQA
Sbjct: 301 LIKDAENDLNPDGSVSNPWNLCSVDQVESLKNFIRIIPVWSTGIIMVVCINQNALGALQA 360

Query: 361 KIMNRHITGSFEFPAGSINVFMIISLSLWLVFYDRIIMPLLKRCGHAGRLSPELRIGIGL 420
           KIMNRHITGSFEFPAGSINVFMIISLSLWLVFYDRIIMPLLKRCGHAGRLSPELRIGIGL
Sbjct: 361 KIMNRHITGSFEFPAGSINVFMIISLSLWLVFYDRIIMPLLKRCGHAGRLSPELRIGIGL 420

Query: 421 LLSCFAMAIAAVVEATRRRIAIEEGLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQI 480
           LLSCFAMAIAAVVEATRRRIAIEEGLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQI
Sbjct: 421 LLSCFAMAIAAVVEATRRRIAIEEGLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQI 480

Query: 481 EYFFSKISKVSSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNETSWLDNNLNKGHLD 540
           EYFFSKISKVSSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNETSWLDNNLNKGHLD
Sbjct: 481 EYFFSKISKVSSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNETSWLDNNLNKGHLD 540

Query: 541 YFYWLCAALALVNLCGYLLYNRAYGFSEKIQGNESSEIQDFEYRNLPSS 589
           YFYWLCAALALVNLCGYLLYNRAYGFSEKIQGNESSEIQDFEYRNLPSS
Sbjct: 541 YFYWLCAALALVNLCGYLLYNRAYGFSEKIQGNESSEIQDFEYRNLPSS 589

BLAST of MC08g_new0030 vs. ExPASy TrEMBL
Match: A0A6J1BSW7 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005055 PE=3 SV=1)

HSP 1 Score: 988 bits (2554), Expect = 0.0
Identity = 490/589 (83.19%), Postives = 540/589 (91.68%), Query Frame = 0

Query: 1   MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIANEVFEKISSLGLSPNMMFYFR 60
           MEVVEN EKTGIA+SS EGTHQASNCRTGGLRTMPFII NE FEKI+SLGL+PNM+FY R
Sbjct: 1   MEVVENEEKTGIAQSSSEGTHQASNCRTGGLRTMPFIIVNETFEKIASLGLTPNMVFYLR 60

Query: 61  DEYGFQIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIILGSFCSFLGMALLWLTA 120
           D+YGFQIAAASS+LSLWSAASNAL+IAGAVLADSY+GRFR IILGSF SFLGMALLWLTA
Sbjct: 61  DDYGFQIAAASSILSLWSAASNALAIAGAVLADSYVGRFRSIILGSFSSFLGMALLWLTA 120

Query: 121 TIPQLKPSSCSSSGILCDSANLYQLAVLFTSLGFISIGAGCIKPCSMAFGADQLTNEKNS 180
            IPQLKPSSCSSSGILCDSAN YQLA+LFTSLGFISIGAGCI+PCS+AFGADQLTNEK  
Sbjct: 121 MIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKP 180

Query: 181 SNKGVLDTYFNWYYAASGISTVIGMTVIVYIQDEFGWGTGYVVPAVLMLLSALIFLAGSS 240
           +N+GVLDTYFNWYYA+SGI++VI +T+IVYIQDEFGW TG+ VPAVLML S LIFL GSS
Sbjct: 181 NNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKVKPAQSLFTGIFQVIVVAFKNRRLSLPYSNFDQYYLGHDPKLLVPTDSMRCLNKAC 300
           LYVKVKP QSL TG+ QVIVVAFKNRRLSLPYSN+DQYYLG DPK LVPTDSMRCLNKAC
Sbjct: 241 LYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTDSMRCLNKAC 300

Query: 301 LIKDAENDLNPDGSVSNPWNLCSVDQVESLKNFIRIIPVWSTGIIMVVCINQNALGALQA 360
           LIKDAENDLNPDGS+SNPWNLCSV+ VESLKNF+RIIP+WSTGI+M+VC+NQN+ G LQA
Sbjct: 301 LIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLNQNSFGTLQA 360

Query: 361 KIMNRHITGSFEFPAGSINVFMIISLSLWLVFYDRIIMPLLKRCGHAGRLSPELRIGIGL 420
           K MNRHITGSFE PAGSI V MIISLS+WL FYDRI++PLL+RCGHAG LSP LR+GIGL
Sbjct: 361 KTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLSPRLRMGIGL 420

Query: 421 LLSCFAMAIAAVVEATRRRIAIEEGLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQI 480
           LLSCFAMAI+AVVE TRRRIA EEGLEDQP AV+DMS LW+VPQCVVLGVAEGFNA+GQI
Sbjct: 421 LLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQI 480

Query: 481 EYFFSKISKVSSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNETSWLDNNLNKGHLD 540
           EYF+S++ K  SS+AVAL+TVEM+VA+LVGS LVQ+VNGITGKGNETSWLDNNLNKGHLD
Sbjct: 481 EYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLD 540

Query: 541 YFYWLCAALALVNLCGYLLYNRAYGFSEKIQGNESSEIQDFEYRNLPSS 589
           YFYWLCAALAL+N  GYLL NR YG SE  + NES+E+QDFEYR LPSS
Sbjct: 541 YFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFEYRKLPSS 589

BLAST of MC08g_new0030 vs. ExPASy TrEMBL
Match: A0A6J1HEG1 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462798 PE=3 SV=1)

HSP 1 Score: 867 bits (2241), Expect = 1.05e-312
Identity = 430/589 (73.01%), Postives = 509/589 (86.42%), Query Frame = 0

Query: 1   MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIANEVFEKISSLGLSPNMMFYFR 60
           MEV+E+ EKTGIA S  E   QASN   GGL+TMPFII NE FE+++SLGL+PNM+FY R
Sbjct: 1   MEVLEDGEKTGIAASGSEEAEQASNYTNGGLKTMPFIIVNETFERVASLGLTPNMVFYLR 60

Query: 61  DEYGFQIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIILGSFCSFLGMALLWLTA 120
           D +GF+IAAAS +LSLWSAASNAL+I GAVLADSY+GRF VIILGS  S LGM LLWLTA
Sbjct: 61  DVFGFEIAAASIILSLWSAASNALAIVGAVLADSYLGRFCVIILGSCSSLLGMILLWLTA 120

Query: 121 TIPQLKPSSCSSSGILCDSANLYQLAVLFTSLGFISIGAGCIKPCSMAFGADQLTNEKNS 180
            IPQL+P+SCS SGILCDSAN YQLAVLF+SL FISIGAGCI+PCS+AFGADQLT E+  
Sbjct: 121 IIPQLRPASCSPSGILCDSANPYQLAVLFSSLVFISIGAGCIRPCSVAFGADQLTKEEKP 180

Query: 181 SNKGVLDTYFNWYYAASGISTVIGMTVIVYIQDEFGWGTGYVVPAVLMLLSALIFLAGSS 240
           +N+ VLD+YFNWYYA+ GI+T+I +++IV+IQD+FGWG G+ VPAVLML S LIFL GSS
Sbjct: 181 NNESVLDSYFNWYYASIGIATIIALSLIVFIQDKFGWGIGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKVKPAQSLFTGIFQVIVVAFKNRRLSLPYSNFDQYYLGHDPKLLVPTDSMRCLNKAC 300
           LYVKVKP QSL TG  +VIVVAFKNR+LSLP+SNFDQYYLG DPK  +PT+SMR LNKAC
Sbjct: 241 LYVKVKPTQSLLTGFLRVIVVAFKNRKLSLPFSNFDQYYLGRDPKCRIPTNSMRYLNKAC 300

Query: 301 LIKDAENDLNPDGSVSNPWNLCSVDQVESLKNFIRIIPVWSTGIIMVVCINQNALGALQA 360
           LIKDA+N+LNPDGSVSNPWNLCSVDQVESLK F+RIIP+WSTGI+M+VC+NQN+ G +QA
Sbjct: 301 LIKDAKNNLNPDGSVSNPWNLCSVDQVESLKAFLRIIPMWSTGILMLVCLNQNSFGTIQA 360

Query: 361 KIMNRHITGSFEFPAGSINVFMIISLSLWLVFYDRIIMPLLKRCGHAGRLSPELRIGIGL 420
           K MNRHIT SFE PAGSI +FM++SL  W++FYDRI++PLL R GHAG LSP+LRIGIGL
Sbjct: 361 KTMNRHITRSFEIPAGSITIFMVLSLFSWIMFYDRILVPLLARHGHAGGLSPKLRIGIGL 420

Query: 421 LLSCFAMAIAAVVEATRRRIAIEEGLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQI 480
           LLSCFAMAI+A V   RRR+AIE+G EDQPNAV++MSALW+VPQCVVLGVAE  N+VGQ+
Sbjct: 421 LLSCFAMAISAGVACIRRRVAIEQGFEDQPNAVINMSALWMVPQCVVLGVAEALNSVGQV 480

Query: 481 EYFFSKISKVSSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNETSWLDNNLNKGHLD 540
           EYF+S++ K  SS+AVAL++ EM+VANLVGSI+VQ+VN ITG+GN+TSWLDNN+NKGHLD
Sbjct: 481 EYFYSQMPKTLSSVAVALYSFEMAVANLVGSIIVQMVNSITGEGNKTSWLDNNVNKGHLD 540

Query: 541 YFYWLCAALALVNLCGYLLYNRAYGFSEKIQGNESSEIQDFEYRNLPSS 589
           YFYWLCAALAL+N  GYL+Y  A G  +K    E +E++DFEYRNLPSS
Sbjct: 541 YFYWLCAALALINFFGYLVYCWALGRQDK----EPNEMEDFEYRNLPSS 585

BLAST of MC08g_new0030 vs. ExPASy TrEMBL
Match: A0A6J1K947 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491783 PE=3 SV=1)

HSP 1 Score: 864 bits (2232), Expect = 1.90e-311
Identity = 429/589 (72.84%), Postives = 509/589 (86.42%), Query Frame = 0

Query: 1   MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIANEVFEKISSLGLSPNMMFYFR 60
           MEV+E+ EKTGI+ S  EGT QASN   GGL+TMPFII NE FE+++SLGL+PNM+FY R
Sbjct: 1   MEVLEDGEKTGISASGSEGTEQASNHTNGGLKTMPFIIVNETFERVASLGLTPNMVFYLR 60

Query: 61  DEYGFQIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIILGSFCSFLGMALLWLTA 120
           D +GFQIA AS +LSLWSAASNAL+I GAVLADSY+GRF VIILGS  S LGM LLWLTA
Sbjct: 61  DVFGFQIADASIILSLWSAASNALAIVGAVLADSYLGRFCVIILGSCSSLLGMILLWLTA 120

Query: 121 TIPQLKPSSCSSSGILCDSANLYQLAVLFTSLGFISIGAGCIKPCSMAFGADQLTNEKNS 180
            IPQL+P+SCS SGILCDSAN YQLAVLF+SL FISIGAGCI+PCS+AFGADQLT E+  
Sbjct: 121 IIPQLRPASCSPSGILCDSANPYQLAVLFSSLVFISIGAGCIRPCSVAFGADQLTKEEKP 180

Query: 181 SNKGVLDTYFNWYYAASGISTVIGMTVIVYIQDEFGWGTGYVVPAVLMLLSALIFLAGSS 240
           +N+ VLD+YFNWYYA+ GI+T+  +++IV+IQD+FGWG G+ VPAVLML S LIFL GSS
Sbjct: 181 NNESVLDSYFNWYYASIGIATIFALSLIVFIQDKFGWGIGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKVKPAQSLFTGIFQVIVVAFKNRRLSLPYSNFDQYYLGHDPKLLVPTDSMRCLNKAC 300
           LYVKVKP QSL TG  +VIVVAFKNR+LSLP+SNFDQYYLG DPK  +PT+SMR LNKAC
Sbjct: 241 LYVKVKPTQSLLTGFLRVIVVAFKNRKLSLPFSNFDQYYLGCDPKCPIPTNSMRYLNKAC 300

Query: 301 LIKDAENDLNPDGSVSNPWNLCSVDQVESLKNFIRIIPVWSTGIIMVVCINQNALGALQA 360
           LIKDA+N+LNPDGSVSNPWNLCSVDQVESLK F+RIIP+WSTGI+M+VC+NQN+ G +QA
Sbjct: 301 LIKDAKNNLNPDGSVSNPWNLCSVDQVESLKAFLRIIPMWSTGILMLVCLNQNSFGTIQA 360

Query: 361 KIMNRHITGSFEFPAGSINVFMIISLSLWLVFYDRIIMPLLKRCGHAGRLSPELRIGIGL 420
           K MNRHIT SFE PAGSI +FM++SL  W++FYDRI++PLL R GHAG LSP+LRIGIGL
Sbjct: 361 KTMNRHITRSFEIPAGSITIFMVLSLFSWIMFYDRILVPLLARYGHAGGLSPKLRIGIGL 420

Query: 421 LLSCFAMAIAAVVEATRRRIAIEEGLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQI 480
           LLSCFAMAI+A V   RRR+AIE+G EDQPNAV++MSALW+VPQCVVLGVAE  N+VGQ+
Sbjct: 421 LLSCFAMAISAGVACIRRRVAIEQGFEDQPNAVINMSALWMVPQCVVLGVAEALNSVGQV 480

Query: 481 EYFFSKISKVSSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNETSWLDNNLNKGHLD 540
           EYF+S++ K+ SS+AVAL++ EM+VANLVGSI+VQ+VN ITG+GN+TSWLDNN+NKGHLD
Sbjct: 481 EYFYSQMPKMLSSVAVALYSFEMAVANLVGSIIVQMVNSITGEGNKTSWLDNNVNKGHLD 540

Query: 541 YFYWLCAALALVNLCGYLLYNRAYGFSEKIQGNESSEIQDFEYRNLPSS 589
           YFYWLCAALAL+N  GYL+   A G  +K    E +E++DFEYRNLPSS
Sbjct: 541 YFYWLCAALALINFFGYLVCCWALGRQDK----EPNEMEDFEYRNLPSS 585

BLAST of MC08g_new0030 vs. ExPASy TrEMBL
Match: A0A6J1BVB4 (protein NRT1/ PTR FAMILY 1.2-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111005055 PE=3 SV=1)

HSP 1 Score: 805 bits (2080), Expect = 9.59e-290
Identity = 394/478 (82.43%), Postives = 436/478 (91.21%), Query Frame = 0

Query: 112 GMALLWLTATIPQLKPSSCSSSGILCDSANLYQLAVLFTSLGFISIGAGCIKPCSMAFGA 171
           GMALLWLTA IPQLKPSSCSSSGILCDSAN YQLA+LFTSLGFISIGAGCI+PCS+AFGA
Sbjct: 20  GMALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGA 79

Query: 172 DQLTNEKNSSNKGVLDTYFNWYYAASGISTVIGMTVIVYIQDEFGWGTGYVVPAVLMLLS 231
           DQLTNEK  +N+GVLDTYFNWYYA+SGI++VI +T+IVYIQDEFGW TG+ VPAVLML S
Sbjct: 80  DQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFS 139

Query: 232 ALIFLAGSSLYVKVKPAQSLFTGIFQVIVVAFKNRRLSLPYSNFDQYYLGHDPKLLVPTD 291
            LIFL GSSLYVKVKP QSL TG+ QVIVVAFKNRRLSLPYSN+DQYYLG DPK LVPTD
Sbjct: 140 VLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTD 199

Query: 292 SMRCLNKACLIKDAENDLNPDGSVSNPWNLCSVDQVESLKNFIRIIPVWSTGIIMVVCIN 351
           SMRCLNKACLIKDAENDLNPDGS+SNPWNLCSV+ VESLKNF+RIIP+WSTGI+M+VC+N
Sbjct: 200 SMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLN 259

Query: 352 QNALGALQAKIMNRHITGSFEFPAGSINVFMIISLSLWLVFYDRIIMPLLKRCGHAGRLS 411
           QN+ G LQAK MNRHITGSFE PAGSI V MIISLS+WL FYDRI++PLL+RCGHAG LS
Sbjct: 260 QNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLS 319

Query: 412 PELRIGIGLLLSCFAMAIAAVVEATRRRIAIEEGLEDQPNAVVDMSALWLVPQCVVLGVA 471
           P LR+GIGLLLSCFAMAI+AVVE TRRRIA EEGLEDQP AV+DMS LW+VPQCVVLGVA
Sbjct: 320 PRLRMGIGLLLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVA 379

Query: 472 EGFNAVGQIEYFFSKISKVSSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNETSWLD 531
           EGFNA+GQIEYF+S++ K  SS+AVAL+TVEM+VA+LVGS LVQ+VNGITGKGNETSWLD
Sbjct: 380 EGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLD 439

Query: 532 NNLNKGHLDYFYWLCAALALVNLCGYLLYNRAYGFSEKIQGNESSEIQDFEYRNLPSS 589
           NNLNKGHLDYFYWLCAALAL+N  GYLL NR YG SE  + NES+E+QDFEYR LPSS
Sbjct: 440 NNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFEYRKLPSS 497

BLAST of MC08g_new0030 vs. TAIR 10
Match: AT1G52190.1 (Major facilitator superfamily protein )

HSP 1 Score: 524.2 bits (1349), Expect = 1.3e-148
Identity = 263/545 (48.26%), Postives = 385/545 (70.64%), Query Frame = 0

Query: 29  GGLRTMPFIIANEVFEKISSLGLSPNMMFYFRDEYGFQIAAASSVLSLWSAASNALSIAG 88
           GG+ TMPFIIANE FEK++S GL PNM+ Y   +Y F +A  ++VL +WSAASN   + G
Sbjct: 23  GGIITMPFIIANEAFEKVASYGLLPNMIMYLIRDYRFGVAKGTNVLFMWSAASNFTPLLG 82

Query: 89  AVLADSYMGRFRVIILGSFCSFLGMALLWLTATIPQLKPSSC--SSSGILCDSANLYQLA 148
           A L+DSY+GRF  I + S  SFLGM LLWLTA +PQ+KPS C  +++G  C S+   QLA
Sbjct: 83  AFLSDSYLGRFLTISIASLSSFLGMVLLWLTAMLPQVKPSPCDPTAAGSHCGSSTASQLA 142

Query: 149 VLFTSLGFISIGAGCIKPCSMAFGADQLTNEKNSSNKGVLDTYFNWYYAASGISTVIGMT 208
           +L+++   ISIG+G I+PCS+AFGADQL N++N  N+ VL+++F WYYA+S ++ +I  T
Sbjct: 143 LLYSAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSAVAVLIAFT 202

Query: 209 VIVYIQDEFGWGTGYVVPAVLMLLSALIFLAGSSLYVKVKPAQSLFTGIFQVIVVAFKNR 268
            IVYIQ+  GW  G+ VPAVLML++AL+F+  S LYV     +SLFTG+ Q IV A+K R
Sbjct: 203 GIVYIQEHLGWKIGFGVPAVLMLIAALLFILASPLYVTRGVTKSLFTGLAQAIVAAYKKR 262

Query: 269 RLSLP--YSNFDQYYLGHDPKLLVPTDSMRCLNKACLIKDAENDLNPDGSVSNPWNLCSV 328
           +LSLP  + +FD YY   D ++  P+  +R LNKACLI + E ++  DG   NPW LC+ 
Sbjct: 263 KLSLPDHHDSFDCYYHMKDSEIKAPSQKLRFLNKACLISNREEEIGSDGFALNPWRLCTT 322

Query: 329 DQVESLKNFIRIIPVWSTGIIMVVCINQNALGALQAKIMNRHIT---GSFEFPAGSINVF 388
           D+VE LK  I++IP+WSTGI+M +  +Q++   LQA  M+R ++    SF+ PAGS  +F
Sbjct: 323 DKVEELKALIKVIPIWSTGIMMSINTSQSSFQLLQATSMDRRLSRHGSSFQVPAGSFGMF 382

Query: 389 MIISLSLWLVFYDRIIMPLLKRC-GHAGRLSPELRIGIGLLLSCFAMAIAAVVEATRRRI 448
            II+L+LW++ YDR ++PL  +  G   RLS +LR+G+GL +S  AMAI+A+VE+ RR+ 
Sbjct: 383 TIIALALWVILYDRAVIPLASKIRGRPFRLSVKLRMGLGLFMSFLAMAISAMVESFRRKK 442

Query: 449 AIEEGLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQIEYFFSKISKVSSSMAVALHT 508
           AI +G  +  NAVVD+SA+WLVPQ V+ G+AE   A+GQ E+F+++  K  SS+A +L  
Sbjct: 443 AISQGYANNSNAVVDISAMWLVPQYVLHGLAEALTAIGQTEFFYTEFPKSMSSIAASLFG 502

Query: 509 VEMSVANLVGSILVQIVNGITGKGNETSWLDNNLNKGHLDYFYWLCAALALVNLCGYLLY 566
           + M+VA+L+ S+++  VN +T +  + SW+ +N+NKGH +Y+YW+ A ++ +N+  Y++ 
Sbjct: 503 LGMAVASLLASVVLNAVNELTSRNGKESWVSDNINKGHYNYYYWVLAIMSFINVIYYVIC 562

BLAST of MC08g_new0030 vs. TAIR 10
Match: AT3G16180.1 (Major facilitator superfamily protein )

HSP 1 Score: 507.3 bits (1305), Expect = 1.7e-143
Identity = 255/543 (46.96%), Postives = 374/543 (68.88%), Query Frame = 0

Query: 29  GGLRTMPFIIANEVFEKISSLGLSPNMMFYFRDEYGFQIAAASSVLSLWSAASNALSIAG 88
           GGL TMPFIIANE FEK++S GL  NM+ Y   +Y   +    +VL +W AA+N + + G
Sbjct: 25  GGLLTMPFIIANEGFEKVASYGLLQNMILYLMSDYRLGLVKGQTVLFMWVAATNFMPLVG 84

Query: 89  AVLADSYMGRFRVIILGSFCSFLGMALLWLTATIPQLKPSSC-SSSGILCDSANLYQLAV 148
           A L+DSY+GRF  I++ S  S LGM +LWLTA +PQ+KPS C +++G  C SA   QLA+
Sbjct: 85  AFLSDSYLGRFLTIVIASLSSLLGMVVLWLTAMLPQVKPSPCVATAGTNCSSATSSQLAL 144

Query: 149 LFTSLGFISIGAGCIKPCSMAFGADQLTNEKNSSNKGVLDTYFNWYYAASGISTVIGMTV 208
           L+T+   ISIG+G I+PCS+AFGADQL N++N  N+ VL+++F WYYA+S ++ +I  TV
Sbjct: 145 LYTAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSSVAVLIAFTV 204

Query: 209 IVYIQDEFGWGTGYVVPAVLMLLSALIFLAGSSLYVKVKPAQSLFTGIFQVIVVAFKNRR 268
           IVYIQD  GW  G+ +PA+LMLL+  +F+  S LYVK   ++SLFTG+ QV+  A+  R 
Sbjct: 205 IVYIQDHLGWKIGFGIPAILMLLAGFLFVFASPLYVKRDVSKSLFTGLAQVVAAAYVKRN 264

Query: 269 LSLP--YSNFDQYYLGHDPKLLVPTDSMRCLNKACLIKDAENDLNPDGSVSNPWNLCSVD 328
           L+LP  + + D YY   D +L  P+D +R LNKAC I + + DL  DG   N W LC+ D
Sbjct: 265 LTLPDHHDSRDCYYRLKDSELKAPSDKLRFLNKACAISNRDEDLGSDGLALNQWRLCTTD 324

Query: 329 QVESLKNFIRIIPVWSTGIIMVVCINQNALGALQAKIMNRHIT--GSFEFPAGSINVFMI 388
           QVE LK  +++IPVWSTGI+M + ++QN+   LQAK M+R ++   +F+ PAGS  +F I
Sbjct: 325 QVEKLKALVKVIPVWSTGIMMSINVSQNSFQLLQAKSMDRRLSSNSTFQIPAGSFGMFTI 384

Query: 389 ISLSLWLVFYDRIIMPLLKRC-GHAGRLSPELRIGIGLLLSCFAMAIAAVVEATRRRIAI 448
           I+L  W+V YDR I+PL  +  G   R++ ++R+G+GL +S  AMA++A VE  RR+ AI
Sbjct: 385 IALISWVVLYDRAILPLASKIRGRPVRVNVKIRMGLGLFISFLAMAVSATVEHYRRKTAI 444

Query: 449 EEGLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQIEYFFSKISKVSSSMAVALHTVE 508
            +GL +  N+ V +SA+WLVPQ V+ G+AE    +GQ E+F+++  K  SS+A +L  + 
Sbjct: 445 SQGLANDANSTVSISAMWLVPQYVLHGLAEALTGIGQTEFFYTEFPKSMSSIAASLFGLG 504

Query: 509 MSVANLVGSILVQIVNGITGKGNETSWLDNNLNKGHLDYFYWLCAALALVNLCGYLLYNR 566
           M+VAN++ S+++  V   + +GN  SW+++N+NKGH DY+YW+ A L+ VN+  Y++ + 
Sbjct: 505 MAVANILASVILNAVKNSSKQGN-VSWIEDNINKGHYDYYYWVLAILSFVNVIYYVVCSW 564

BLAST of MC08g_new0030 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 391.0 bits (1003), Expect = 1.7e-108
Identity = 217/566 (38.34%), Postives = 332/566 (58.66%), Query Frame = 0

Query: 27  RTGGLRTMPFIIANEVFEKISSLGLSPNMMFYFRDEYGFQIAAASSVLSLWSAASNALSI 86
           + GG R + FI+ NE  E++ S+GL  N M Y    +  +   A++V+++WS  +N   +
Sbjct: 51  KPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPL 110

Query: 87  AGAVLADSYMGRFRVIILGSFCSFLGMALLWLTATIPQLKPSSCSSSGIL-CDSANLYQL 146
            GA ++D+Y+GRF+ I   SF + LG+  + LTA+ PQL P+SC+S   L C   N  Q+
Sbjct: 111 VGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQI 170

Query: 147 AVLFTSLGFISIGAGCIKPCSMAFGADQLTNEKNSSNKGVLDTYFNWYYAASGISTVIGM 206
            VL   L F+S+G+G I+PCS+ FG DQ         KGV  ++FNWYY    +  +I  
Sbjct: 171 GVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVA-SFFNWYYMTFTVVLIITQ 230

Query: 207 TVIVYIQDEFGWGTGYVVPAVLMLLSALIFLAGSSLYVKVKPAQSLFTGIFQVIVVAFKN 266
           TV+VYIQD+  W  G+ +P  LM L+ ++F AG   YV VKP  S+F+GI QVIV A K 
Sbjct: 231 TVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKK 290

Query: 267 RRLSLPYSNFDQYYLGHDPKL-------LVPTDSMRCLNKACLIKDAENDLNPDGSVSNP 326
           R+L LP  + D     +DP +       L  ++  RCL+KA ++   E DL P+G  ++ 
Sbjct: 291 RKLKLPAED-DGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVV--IEGDLTPEGPPADK 350

Query: 327 WNLCSVDQVESLKNFIRIIPVWSTGIIMVVCI-NQNALGALQAKIMNRHITGSFEFPAGS 386
           W LCSV +VE +K  IRI+P+WS GII +  +  Q      QA  M+R++   FE PAGS
Sbjct: 351 WRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGS 410

Query: 387 INVFMIISLSLWLVFYDRIIMPLLKR-CGHAGRLSPELRIGIGLLLSCFAMAIAAVVEAT 446
           ++V  ++++ ++L FYDR+ +P ++R  GH   ++   RIG G++ + F+M +A +VE  
Sbjct: 411 LSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERM 470

Query: 447 RRRIAIEEGLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQIEYFFSKISKVSSSMAV 506
           RR  +I  G    P  +  MS  WL PQ +++G+ E FN +GQIE+F S+  +   S+A 
Sbjct: 471 RRIRSINAG---DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIAN 530

Query: 507 ALHTVEMSVANLVGSILVQIVNGITGKGNETSWLDNNLNKGHLDYFYWLCAALALVNLCG 566
           +L ++  + ++ + S LV +V+  +G  +   WL+ NLN G LDYFY+L A L +VNL  
Sbjct: 531 SLFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGVVNLVY 590

Query: 567 YLLYNRAYGFSEKIQGNESSEIQDFE 583
           +    R Y +   +       I+DFE
Sbjct: 591 FWYCARGYRYKVGL------PIEDFE 603

BLAST of MC08g_new0030 vs. TAIR 10
Match: AT1G68570.1 (Major facilitator superfamily protein )

HSP 1 Score: 363.2 bits (931), Expect = 3.9e-100
Identity = 217/569 (38.14%), Postives = 336/569 (59.05%), Query Frame = 0

Query: 21  HQASNCRTGGLRTMPFIIANEVFEKISSLGLSPNMMFYFRDEYGFQIAAASSVLSLWSAA 80
           H   N   GGL TMPFI ANE+ EK++ +G   NM+ Y   +    +  A++ L+ ++  
Sbjct: 17  HGRPNRPKGGLITMPFIFANEICEKLAVVGFHANMISYLTTQLHLPLTKAANTLTNFAGT 76

Query: 81  SNALSIAGAVLADSYMGRFRVIILGSFCSFLGMALLWLTATIPQLKPSSCSSSGILCDSA 140
           S+   + GA +ADS+ GRF  I   S    +GM LL ++A IP L+P  C    + C  A
Sbjct: 77  SSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPTLRPPPCKGEEV-CVVA 136

Query: 141 NLYQLAVLFTSLGFISIGAGCIKPCSMAFGADQLTNEKNSSNKGVLDTYFNWYYAASGIS 200
           +  QL++L+ +L   ++G+G I+PC +AFGADQ  +E + +       YFNWYY   G +
Sbjct: 137 DTAQLSILYVALLLGALGSGGIRPCVVAFGADQF-DESDPNQTTKTWNYFNWYYFCMGAA 196

Query: 201 TVIGMTVIVYIQDEFGWGTGYVVPAVLMLLSALIFLAGSSLYVKVKPAQSLFTGIFQVIV 260
            ++ +TV+V+IQD  GWG G  +P V M LS + F+ G  LY  + PA S FT + QV V
Sbjct: 197 VLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRHLVPAGSPFTRLIQVGV 256

Query: 261 VAFKNRRL------SLPYSNFD---QYYLGHDPKLLVPTDSMRCLNKACLIKDAENDLNP 320
            AF+ R+L      SL Y N +      LG     L  T  M  L+KA ++ + E++L P
Sbjct: 257 AAFRKRKLRMVSDPSLLYFNDEIDAPISLGGK---LTHTKHMSFLDKAAIVTE-EDNLKP 316

Query: 321 DGSVSNPWNLCSVDQVESLKNFIRIIPVWSTGIIMVVCINQNALGAL-QAKIMNRHITGS 380
            G + N W L +V +VE LK+ IR+ P+ ++GI+++    Q    +L QAK MNRH+T S
Sbjct: 317 -GQIPNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQGTFSLQQAKTMNRHLTNS 376

Query: 381 FEFPAGSINVFMIISLSLWLVFYDRIIMPLLKR-CGHAGRLSPELRIGIGLLLSCFAMAI 440
           F+ PAGS++VF  +++   ++FYDR+ + + ++  G    ++   R+GIG ++S  A  +
Sbjct: 377 FQIPAGSMSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITFLHRMGIGFVISIIATLV 436

Query: 441 AAVVEATRRRIAIEEGLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQIEYFFSKISK 500
           A  VE  R+ +AIE GL D+P+ +V +S LWL+PQ  + GVAE F ++G +E+F+ +  +
Sbjct: 437 AGFVEVKRKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFMSIGHLEFFYDQAPE 496

Query: 501 VSSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNETSWL-DNNLNKGHLDYFYWLCAA 560
              S A AL  + +S+ N V ++LV +V+  + K + ++WL DNNLN+G L+YFYWL   
Sbjct: 497 SMRSTATALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNLNRGRLEYFYWLITV 556

Query: 561 LALVNLCGYLLYNRAYGFSEKIQGNESSE 578
           L  VNL  YL   + Y + + +Q + S E
Sbjct: 557 LQAVNLVYYLWCAKIYTY-KPVQVHHSKE 577

BLAST of MC08g_new0030 vs. TAIR 10
Match: AT1G18880.1 (Major facilitator superfamily protein )

HSP 1 Score: 348.6 bits (893), Expect = 9.9e-96
Identity = 196/563 (34.81%), Postives = 324/563 (57.55%), Query Frame = 0

Query: 30  GLRTMPFIIANEVFEKISSLGLSPNMMFYFRDEYGFQIAAASSVLSLWSAASNALSIAGA 89
           G + MPFII NE FEK+  +G S N++ Y    +  +   A+ V++++   SN  +I  A
Sbjct: 22  GWKVMPFIIGNETFEKLGIVGSSSNLVIYLTTVFNMKSITAAKVVNIYGGTSNFGTIVAA 81

Query: 90  VLADSYMGRFRVIILGSFCSFLGMALLWLTATIPQLKPSSCSSS-GILCDSANLYQLAVL 149
            L DSY GR++ +       FLG   + LTA I  L P+ C+   G +C+  ++ Q+  L
Sbjct: 82  FLCDSYFGRYKTLSFAMIACFLGSVAMDLTAVIHPLHPAQCAKEIGSVCNGPSIGQIMFL 141

Query: 150 FTSLGFISIGAGCIKPCSMAFGADQLTNEKNSSNKGVLDTYFNWYYAASGISTVIGMTVI 209
             ++  + IGAG I+PC++ FGADQ   +     +G+ +++FNWY+     + ++ +T+I
Sbjct: 142 AGAMVLLVIGAGGIRPCNLPFGADQFDPKTKEGKRGI-ESFFNWYFFTFTFAQMVSLTLI 201

Query: 210 VYIQDEFGWGTGYVVPAVLMLLSALIFLAGSSLYVKVKPAQSLFTGIFQVIVVAFKNRRL 269
           VY+Q    W  G  +PA+LMLL  +IF AGS LYVKVK + S    I +VIVVA K RRL
Sbjct: 202 VYVQSNVSWSIGLAIPAILMLLGCIIFFAGSKLYVKVKASGSPIHSITRVIVVAIKKRRL 261

Query: 270 SLPYSNFDQYYLGHDPK--LLVPTDSMRCLNKACLIKDAENDLNPDGSVSNPWNLCSVDQ 329
                N    Y+  D K   L  T+  R L+K+  I+  ++ LN DGS  + W LCS+ Q
Sbjct: 262 KPVGPNELYNYIASDFKNSKLGHTEQFRFLDKSA-IQTQDDKLNKDGSPVDAWKLCSMQQ 321

Query: 330 VESLKNFIRIIPVW-STGIIMVVCINQNALGALQAKIMNRHI-TGSFEFPAGSINVFMII 389
           VE +K  IR++PVW S  +  +  I Q      Q+   +R +  GSF+ PAGS  VF+++
Sbjct: 322 VEEVKCVIRVLPVWLSAALFYLAYIQQTTYTIFQSLQSDRRLGPGSFQIPAGSYTVFLML 381

Query: 390 SLSLWLVFYDRIIMPLLKR-CGHAGRLSPELRIGIGLLLSCFAMAIAAVVEATRRRIAIE 449
            +++++  YDR+++P L++  G  G ++   R+G GL L   +M ++A+VE  RR++A+ 
Sbjct: 382 GMTIFIPIYDRVLVPFLRKYTGRDGGITQLQRVGAGLFLCITSMMVSAIVEQYRRKVALT 441

Query: 450 E---GLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQIEYFFSKISKVSSSMAVALHT 509
           +   GL  +  A+  MS +WL+PQ V++G+A+    VGQ+E+++ +  +   S A +L+ 
Sbjct: 442 KPTLGLAPRKGAISSMSGMWLIPQLVLMGIADALAGVGQMEFYYKQFPENMRSFAGSLYY 501

Query: 510 VEMSVANLVGSILVQIVNGITGKGNETSWLDNNLNKGHLDYFYWLCAALALVNLCGYLLY 569
             + +A+ + + L+  V+  T   +  SWL  +LNKG L+YFY+L A +  +NL  +LL 
Sbjct: 502 CGIGLASYLSTFLLSAVHDTTEGFSGGSWLPEDLNKGRLEYFYFLVAGMMTLNLAYFLLV 561

Query: 570 NRAYGFSEKIQGNESSEIQDFEY 584
           +  Y + + +  ++  +    E+
Sbjct: 562 SHWYRYKDVVAKDKDMDKTSAEF 582