MC08g_new0028 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g_new0028
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionprotein NRT1/ PTR FAMILY 1.2-like
LocationMC08: 2398978 .. 2402518 (+)
RNA-Seq ExpressionMC08g_new0028
SyntenyMC08g_new0028
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGCACTTCAATACTGAAGTCTAATGTTACCCCGATATCTTTCCTTTTTAATTTCTTAGATTAGATGGGAAAAGTAAAATTAACAATTTGTATTTTTATGAATACATGTTTAATCTTGGTAGATTCCACCTTCCACAATAAGCGGCAGTCGCTATATCTTGACATTGCATTGAATATATTTTTTTTTCTTATTTTGATTTCGACATTTCATTGAATTTGATTGGTTTCGTTCAGTTCTTATTTTTCGACCTGCCTCTGCAGAGTAATGAGCGACTCTTTCAATAATTTTTCGAGAGAGAGAGTGGAATCGAGAACTGTCTGTGATTGTTGAAGGTCGCGAGATTGTTGGAGAGAATGGAGGTTGTAGAAAATGAGGAGAAGACAGGAATCGCCCAAAGCAGTAGCGAGGGAACCCATCAAGCTTCGAATTGCAGAACTGGCGGATTAAGAACAATGCCCTTCATAATCGGTATTCAGTTCGATTCCGTCTCTGATTTTAGTAGCTTCGCTTTCTAAATTAGATTTATGATGAGCTCTCTGTGTCTTTGTGGCTTTGCTTGCGTCTATTTCCGGCAGTTAATGAAACTTTTGAGAAGATCGCAAGTTTAGGGCTAACTCCTAACATGGTGTTCTACTTGAGGGACGATTACGGGTTTCAGATTGCTGCTGCTTCTAGCATTCTGTCTCTGTGGTCTGCTGCTTCCAATGCTTTGGCCATTGCTGGAGCTGTTCTGGCTGATTCTTACGTGGGGCGTTTCCGCAGTATAATTCTCGGATCCTTCAGTAGCTTTCTTGTAAGCCTGTTGATTGTCGAAATTGAACTGTTTCCATTGATGTAATACTAATACACTTTTGGTTAAGAAACACATTACCCTCGTACTTTCATTAATTTAGTTCCTAAACTTTCTGTAATAACTTAGTCTTTTTGTAGTTTAAAATCTTAGTTTGATCGTGAAATACAAAATTTTTCATGGTACGGGCTAGATTGTTTATAAATTTTGAACTACAAGAACTGAGGAACTAAATTATGTGGTGTTTAAACGTTATTTTTGTTGTTTCGGAAAGGGGAGCTTTTTAATACATCACTGTTGTGTGGTATAGTTAAAGAACTGCATGCTTGGGTCTGGACTTTTGCTGCTTGCTTGTTTTGTGGTATAGTTAAAGAAGACTTATAGGATCAATACAGAGCATATGAACCTCCAAGACTCTCTGGGCCTTAAATCCCCTTGCTTGTGTCATTGATCACTTGATAGTTTGAGTTATATTTTATATAGAACTTCTTTTAAGGACAAGCTCTATATTTTGCTCCAGCTGCGTGTGTAAAATTATGTGGTTTGCTTTATTTCTTCAGGGGATGGCTCTATTGTGGTTAACAGCAATGATCCCACAACTGAAACCTTCATCTTGTTCATCCTCTGGAATATTGTGCGACTCTGCTAATCCGTACCAGCTCGCGATACTGTTCACTTCGTTGGGATTTATTTCCATTGGAGCGGGTTGCATTAGACCATGTTCTATCGCGTTTGGTGCAGACCAACTCACCAATGAGAAAAAGCCCAATAACGAGGGTGTCTTAGATACCTACTTCAATTGGTATTATGCATCAAGTGGGATAGCTTCAGTTATCGCATTAACTCTTATTGTGTATATTCAAGATGAATTTGGATGGAGAACTGGATTTGCAGTTCCTGCGGTTCTCATGCTTTTTTCTGTGCTAATATTTCTAGTGGGTTCTTCTCTATATGTGAAAGTGAAGCCTACACAGAGCTTGTTAACTGGGCTTTTACAAGTAATTGTGGTGGCATTTAAGAACAGAAGATTGTCGCTCCCTTATAGTAACTATGACCAGTACTACCTTGGCCCTGATCCGAAGTTCCTTGTCCCAACTGATTCTATGAGGTATGTATCTCAATGTCATGTTCGTCACTATTGTTATGTTCTTGAATTTGCCATATTATTTTCTGCTGAGAATTATGTCCTGATGTCTAAATCATTGCCTGTGAAATGAGATTTCCGTTCTGCACTTTTTATTGAGTCATTTTCAATAAATTATTGAATTATTGTCTCGACTCGATAGTCATTGAATCATATCCATTAGATGAATTGCTTCTTAAAGTAACTTAAAGTTGTCTTTCGCTTCAAAGTTACAGGTTTGTGAAAGTTTAATAAGAGAGAGGTGAGGGGATTTGAACGTCCCACCTTTAGGGAGATAAATAATGTCTTGACTAGTCGTGTGATGCTCAAATGCATAATAATTTTTAACTTCTACAACCTATTCAAAAGTAGTTAAATACAGTTTTGATAAACAAAAACAGAAACTCATGTCCAAAGCAGCTCCTTTGATTCATAAAAAGTAACACTAGGCACTCTTGATTTATCCATTTACTATTGACAATGTCACAATTCCCAGTTATGTTCCTAAGAAGATGAAAAGTCTTATCAGCACAATTCCCCTTCCTTCAGGTGTCTTAATAAAGCCTGTCTTATAAAAGATGCTGAGAATGATTTAAATCCAGATGGCTCAATATCAAATCCATGGAACCTGTGCTCTGTGGAACATGTAGAGTCGCTAAAAAATTTTCTAAGAATCATCCCAATGTGGTCAACAGGCATTCTCATGCTTGTCTGCTTAAATCAAAACTCATTTGGCACACTCCAAGCCAAGACTATGAACAGGCACATCACTGGTAGTTTCGAAATCCCCGCAGGCTCAATTGGTGTGTGCATGATTATTTCTTTATCCGTGTGGCTTGCCTTTTATGACAGAATAATGGTGCCTTTATTGGAAAGGTGTGGTCATGCTGGGGGACTCAGTCCTAGATTGAGGATGGGAATTGGTCTGCTTCTCTCGTGCTTTGCAATGGCCATTTCAGCGGTCGTTGAAGGTACAAGGCGTAGAATTGCCACAGAAGGTCTAGAAGACCAACCATATGCTGTCATTGACATGTCAGTTCTATGGATAGTGCCTCAATGTGTAGTCCTGGGAGTTGCCGAAGGTTTCAATGCAATCGGACAGATCGAGTATTTCTACTCGCAAATGCCGAAGACGTTGTCGAGCGTGGCAGTTGCTCTATACACGGTTGAGATGGCTGTAGCTAGCTTGGTGGGAAGCGCTCTTGTGCAGATGGTAAATGGCATAACAGGAAAAGGAAATGAAACAAGCTGGTTGGATAACAATCTCAATAAGGGTCACTTGGATTATTTTTACTGGTTATGTGCTGCCTTGGCTTTGATGAACTTTGTTGGTTATCTTCTATGTAACAGAACTTATGGATTGTCTGAGAACAGACGATGCAATGAATCAAATGAGGTACAAGACTTTGAGTATAGGAAATTACCTTCATCTTAGAGATGGAAAAGATTGCATTCTTCTTCAGATTTGGATGTACCATAGGACCATAGGTCCATAACTATGATATTCAGCTGCATTTGAATCTTCTCTTCATTTATGCTTTTGACTTCAACTATATTTCTGAGCAATGTGAGCATCCTTTTATTTCCTTTATTTGGAGATAGTCTCATTTTGGTATCTTTTGGCCCA

mRNA sequence

ATGCTTTGGCCATTGCTGGAGCTGTTCTGGCTGATTCTTACGTGGGGCGTTTCCGCAGGGATGGCTCTATTGTGGTTAACAGCAATGATCCCACAACTGAAACCTTCATCTTGTTCATCCTCTGGAATATTGTGCGACTCTGCTAATCCGTACCAGCTCGCGATACTGTTCACTTCGTTGGGATTTATTTCCATTGGAGCGGGTTGCATTAGACCATGTTCTATCGCGTTTGGTGCAGACCAACTCACCAATGAGAAAAAGCCCAATAACGAGGGTGTCTTAGATACCTACTTCAATTGGTATTATGCATCAAGTGGGATAGCTTCAGTTATCGCATTAACTCTTATTGTGTATATTCAAGATGAATTTGGATGGAGAACTGGATTTGCAGTTCCTGCGGTTCTCATGCTTTTTTCTGTGCTAATATTTCTAGTGGGTTCTTCTCTATATGTGAAAGTGAAGCCTACACAGAGCTTGTTAACTGGGCTTTTACAAGTAATTGTGGTGGCATTTAAGAACAGAAGATTGTCGCTCCCTTATAGTAACTATGACCAGTACTACCTTGGCCCTGATCCGAAGTTCCTTGTCCCAACTGATTCTATGAGGTGTCTTAATAAAGCCTGTCTTATAAAAGATGCTGAGAATGATTTAAATCCAGATGGCTCAATATCAAATCCATGGAACCTGTGCTCTGTGGAACATGTAGAGTCGCTAAAAAATTTTCTAAGAATCATCCCAATGTGGTCAACAGGCATTCTCATGCTTGTCTGCTTAAATCAAAACTCATTTGGCACACTCCAAGCCAAGACTATGAACAGGCACATCACTGGTAGTTTCGAAATCCCCGCAGGCTCAATTGGTGTGTGCATGATTATTTCTTTATCCGTGTGGCTTGCCTTTTATGACAGAATAATGGTGCCTTTATTGGAAAGGTGTGGTCATGCTGGGGGACTCAGTCCTAGATTGAGGATGGGAATTGGTCTGCTTCTCTCGTGCTTTGCAATGGCCATTTCAGCGGTCGTTGAAGGTACAAGGCGTAGAATTGCCACAGAAGGTCTAGAAGACCAACCATATGCTGTCATTGACATGTCAGTTCTATGGATAGTGCCTCAATGTGTAGTCCTGGGAGTTGCCGAAGGTTTCAATGCAATCGGACAGATCGAGTATTTCTACTCGCAAATGCCGAAGACGTTGTCGAGCGTGGCAGTTGCTCTATACACGGTTGAGATGGCTGTAGCTAGCTTGGTGGGAAGCGCTCTTGTGCAGATGGTAAATGGCATAACAGGAAAAGGAAATGAAACAAGCTGGTTGGATAACAATCTCAATAAGGGTCACTTGGATTATTTTTACTGGTTATGTGCTGCCTTGGCTTTGATGAACTTTGTTGGTTATCTTCTATGTAACAGAACTTATGGATTGTCTGAGAACAGACGATGCAATGAATCAAATGAGGTACAAGACTTTGAGTATAGGAAATTACCTTCATCTTAG

Coding sequence (CDS)

ATGCTTTGGCCATTGCTGGAGCTGTTCTGGCTGATTCTTACGTGGGGCGTTTCCGCAGGGATGGCTCTATTGTGGTTAACAGCAATGATCCCACAACTGAAACCTTCATCTTGTTCATCCTCTGGAATATTGTGCGACTCTGCTAATCCGTACCAGCTCGCGATACTGTTCACTTCGTTGGGATTTATTTCCATTGGAGCGGGTTGCATTAGACCATGTTCTATCGCGTTTGGTGCAGACCAACTCACCAATGAGAAAAAGCCCAATAACGAGGGTGTCTTAGATACCTACTTCAATTGGTATTATGCATCAAGTGGGATAGCTTCAGTTATCGCATTAACTCTTATTGTGTATATTCAAGATGAATTTGGATGGAGAACTGGATTTGCAGTTCCTGCGGTTCTCATGCTTTTTTCTGTGCTAATATTTCTAGTGGGTTCTTCTCTATATGTGAAAGTGAAGCCTACACAGAGCTTGTTAACTGGGCTTTTACAAGTAATTGTGGTGGCATTTAAGAACAGAAGATTGTCGCTCCCTTATAGTAACTATGACCAGTACTACCTTGGCCCTGATCCGAAGTTCCTTGTCCCAACTGATTCTATGAGGTGTCTTAATAAAGCCTGTCTTATAAAAGATGCTGAGAATGATTTAAATCCAGATGGCTCAATATCAAATCCATGGAACCTGTGCTCTGTGGAACATGTAGAGTCGCTAAAAAATTTTCTAAGAATCATCCCAATGTGGTCAACAGGCATTCTCATGCTTGTCTGCTTAAATCAAAACTCATTTGGCACACTCCAAGCCAAGACTATGAACAGGCACATCACTGGTAGTTTCGAAATCCCCGCAGGCTCAATTGGTGTGTGCATGATTATTTCTTTATCCGTGTGGCTTGCCTTTTATGACAGAATAATGGTGCCTTTATTGGAAAGGTGTGGTCATGCTGGGGGACTCAGTCCTAGATTGAGGATGGGAATTGGTCTGCTTCTCTCGTGCTTTGCAATGGCCATTTCAGCGGTCGTTGAAGGTACAAGGCGTAGAATTGCCACAGAAGGTCTAGAAGACCAACCATATGCTGTCATTGACATGTCAGTTCTATGGATAGTGCCTCAATGTGTAGTCCTGGGAGTTGCCGAAGGTTTCAATGCAATCGGACAGATCGAGTATTTCTACTCGCAAATGCCGAAGACGTTGTCGAGCGTGGCAGTTGCTCTATACACGGTTGAGATGGCTGTAGCTAGCTTGGTGGGAAGCGCTCTTGTGCAGATGGTAAATGGCATAACAGGAAAAGGAAATGAAACAAGCTGGTTGGATAACAATCTCAATAAGGGTCACTTGGATTATTTTTACTGGTTATGTGCTGCCTTGGCTTTGATGAACTTTGTTGGTTATCTTCTATGTAACAGAACTTATGGATTGTCTGAGAACAGACGATGCAATGAATCAAATGAGGTACAAGACTTTGAGTATAGGAAATTACCTTCATCTTAG

Protein sequence

MLWPLLELFWLILTWGVSAGMALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFSVLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTDSMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLNQNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLSPRLRMGIGLLLSCFAMAISAVVEGTRRRIATEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFEYRKLPSS
Homology
BLAST of MC08g_new0028 vs. ExPASy Swiss-Prot
Match: Q9M817 (Protein NRT1/ PTR FAMILY 1.2 OS=Arabidopsis thaliana OX=3702 GN=NPF1.2 PE=1 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 1.2e-126
Identity = 229/480 (47.71%), Postives = 336/480 (70.00%), Query Frame = 0

Query: 20  GMALLWLTAMIPQLKPSSC--SSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAF 79
           GM LLWLTAM+PQ+KPS C  +++G  C S+   QLA+L+++   ISIG+G IRPCS+AF
Sbjct: 106 GMVLLWLTAMLPQVKPSPCDPTAAGSHCGSSTASQLALLYSAFALISIGSGGIRPCSLAF 165

Query: 80  GADQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLML 139
           GADQL N++ P NE VL+++F WYYASS +A +IA T IVYIQ+  GW+ GF VPAVLML
Sbjct: 166 GADQLDNKENPKNERVLESFFGWYYASSAVAVLIAFTGIVYIQEHLGWKIGFGVPAVLML 225

Query: 140 FSVLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLP--YSNYDQYYLGPDPKFL 199
            + L+F++ S LYV    T+SL TGL Q IV A+K R+LSLP  + ++D YY   D +  
Sbjct: 226 IAALLFILASPLYVTRGVTKSLFTGLAQAIVAAYKKRKLSLPDHHDSFDCYYHMKDSEIK 285

Query: 200 VPTDSMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILML 259
            P+  +R LNKACLI + E ++  DG   NPW LC+ + VE LK  +++IP+WSTGI+M 
Sbjct: 286 APSQKLRFLNKACLISNREEEIGSDGFALNPWRLCTTDKVEELKALIKVIPIWSTGIMMS 345

Query: 260 VCLNQNSFGTLQAKTMNRHIT---GSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERC 319
           +  +Q+SF  LQA +M+R ++    SF++PAGS G+  II+L++W+  YDR ++PL  + 
Sbjct: 346 INTSQSSFQLLQATSMDRRLSRHGSSFQVPAGSFGMFTIIALALWVILYDRAVIPLASKI 405

Query: 320 -GHAGGLSPRLRMGIGLLLSCFAMAISAVVEGTRRRIA-TEGLEDQPYAVIDMSVLWIVP 379
            G    LS +LRMG+GL +S  AMAISA+VE  RR+ A ++G  +   AV+D+S +W+VP
Sbjct: 406 RGRPFRLSVKLRMGLGLFMSFLAMAISAMVESFRRKKAISQGYANNSNAVVDISAMWLVP 465

Query: 380 QCVVLGVAEGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGK 439
           Q V+ G+AE   AIGQ E+FY++ PK++SS+A +L+ + MAVASL+ S ++  VN +T +
Sbjct: 466 QYVLHGLAEALTAIGQTEFFYTEFPKSMSSIAASLFGLGMAVASLLASVVLNAVNELTSR 525

Query: 440 GNETSWLDNNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYG-LSENRRCNESNEVQDFE 490
             + SW+ +N+NKGH +Y+YW+ A ++ +N + Y++C+ +YG L +  R    N V++ E
Sbjct: 526 NGKESWVSDNINKGHYNYYYWVLAIMSFINVIYYVICSWSYGPLVDQVRNGRVNGVREEE 585

BLAST of MC08g_new0028 vs. ExPASy Swiss-Prot
Match: Q8LPL2 (Protein NRT1/ PTR FAMILY 1.1 OS=Arabidopsis thaliana OX=3702 GN=NPF1.1 PE=1 SV=2)

HSP 1 Score: 438.0 bits (1125), Expect = 1.5e-121
Identity = 215/460 (46.74%), Postives = 321/460 (69.78%), Query Frame = 0

Query: 20  GMALLWLTAMIPQLKPSSC-SSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFG 79
           GM +LWLTAM+PQ+KPS C +++G  C SA   QLA+L+T+   ISIG+G IRPCS+AFG
Sbjct: 108 GMVVLWLTAMLPQVKPSPCVATAGTNCSSATSSQLALLYTAFALISIGSGGIRPCSLAFG 167

Query: 80  ADQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLF 139
           ADQL N++ P NE VL+++F WYYASS +A +IA T+IVYIQD  GW+ GF +PA+LML 
Sbjct: 168 ADQLDNKENPKNERVLESFFGWYYASSSVAVLIAFTVIVYIQDHLGWKIGFGIPAILMLL 227

Query: 140 SVLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLP--YSNYDQYYLGPDPKFLV 199
           +  +F+  S LYVK   ++SL TGL QV+  A+  R L+LP  + + D YY   D +   
Sbjct: 228 AGFLFVFASPLYVKRDVSKSLFTGLAQVVAAAYVKRNLTLPDHHDSRDCYYRLKDSELKA 287

Query: 200 PTDSMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLV 259
           P+D +R LNKAC I + + DL  DG   N W LC+ + VE LK  +++IP+WSTGI+M +
Sbjct: 288 PSDKLRFLNKACAISNRDEDLGSDGLALNQWRLCTTDQVEKLKALVKVIPVWSTGIMMSI 347

Query: 260 CLNQNSFGTLQAKTMNRHIT--GSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERC-G 319
            ++QNSF  LQAK+M+R ++   +F+IPAGS G+  II+L  W+  YDR ++PL  +  G
Sbjct: 348 NVSQNSFQLLQAKSMDRRLSSNSTFQIPAGSFGMFTIIALISWVVLYDRAILPLASKIRG 407

Query: 320 HAGGLSPRLRMGIGLLLSCFAMAISAVVEGTRRRIA-TEGLEDQPYAVIDMSVLWIVPQC 379
               ++ ++RMG+GL +S  AMA+SA VE  RR+ A ++GL +   + + +S +W+VPQ 
Sbjct: 408 RPVRVNVKIRMGLGLFISFLAMAVSATVEHYRRKTAISQGLANDANSTVSISAMWLVPQY 467

Query: 380 VVLGVAEGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGN 439
           V+ G+AE    IGQ E+FY++ PK++SS+A +L+ + MAVA+++ S ++  V   + +GN
Sbjct: 468 VLHGLAEALTGIGQTEFFYTEFPKSMSSIAASLFGLGMAVANILASVILNAVKNSSKQGN 527

Query: 440 ETSWLDNNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYG 473
             SW+++N+NKGH DY+YW+ A L+ +N + Y++C+ +YG
Sbjct: 528 -VSWIEDNINKGHYDYYYWVLAILSFVNVIYYVVCSWSYG 566

BLAST of MC08g_new0028 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 4.9e-93
Identity = 190/483 (39.34%), Postives = 284/483 (58.80%), Query Frame = 0

Query: 20  GMALLWLTAMIPQLKPSSCSSSGIL-CDSANPYQLAILFTSLGFISIGAGCIRPCSIAFG 79
           G+  + LTA  PQL P+SC+S   L C   N  Q+ +L   L F+S+G+G IRPCSI FG
Sbjct: 136 GLITITLTASFPQLHPASCNSQDPLSCGGPNKLQIGVLLLGLCFLSVGSGGIRPCSIPFG 195

Query: 80  ADQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLF 139
            DQ     +   +GV  ++FNWYY +  +  +I  T++VYIQD+  W  GF++P  LM  
Sbjct: 196 VDQFDQRTEEGVKGVA-SFFNWYYMTFTVVLIITQTVVVYIQDQVSWIIGFSIPTGLMAL 255

Query: 140 SVLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSN------YDQYYLGPDP 199
           +V++F  G   YV VKP  S+ +G+ QVIV A K R+L LP  +      YD        
Sbjct: 256 AVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKRKLKLPAEDDGTVTYYDPAIKSSVL 315

Query: 200 KFLVPTDSMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGI 259
             L  ++  RCL+KA ++   E DL P+G  ++ W LCSV+ VE +K  +RI+P+WS GI
Sbjct: 316 SKLHRSNQFRCLDKAAVV--IEGDLTPEGPPADKWRLCSVQEVEEVKCLIRIVPIWSAGI 375

Query: 260 LMLVCL-NQNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLER 319
           + L  +  Q +F   QA  M+R++   FEIPAGS+ V  ++++ ++L FYDR+ VP + R
Sbjct: 376 ISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSLSVISLLTIGIFLPFYDRVFVPFMRR 435

Query: 320 -CGHAGGLSPRLRMGIGLLLSCFAMAISAVVEGTRR-RIATEGLEDQPYAVIDMSVLWIV 379
             GH  G++   R+G G++ + F+M ++ +VE  RR R    G    P  +  MSV W+ 
Sbjct: 436 ITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMRRIRSINAG---DPTGMTPMSVFWLS 495

Query: 380 PQCVVLGVAEGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITG 439
           PQ +++G+ E FN IGQIE+F SQ P+ + S+A +L+++  A +S + S LV +V+  +G
Sbjct: 496 PQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSLFSLSFAGSSYLSSFLVTVVHKFSG 555

Query: 440 KGNETSWLDNNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFE 493
             +   WL+ NLN G LDYFY+L A L ++N V +  C R Y      R      ++DFE
Sbjct: 556 GHDRPDWLNKNLNAGKLDYFYYLIAVLGVVNLVYFWYCARGY------RYKVGLPIEDFE 606

BLAST of MC08g_new0028 vs. ExPASy Swiss-Prot
Match: Q9SX20 (Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 1.5e-86
Identity = 191/478 (39.96%), Postives = 285/478 (59.62%), Query Frame = 0

Query: 9   FWLILTWGV--SAGMALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIG 68
           FW I    +    GM LL ++A+IP L+P  C    + C  A+  QL+IL+ +L   ++G
Sbjct: 95  FWTITFASIIYQIGMTLLTISAIIPTLRPPPCKGEEV-CVVADTAQLSILYVALLLGALG 154

Query: 69  AGCIRPCSIAFGADQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWR 128
           +G IRPC +AFGADQ  +E  PN       YFNWYY   G A ++A+T++V+IQD  GW 
Sbjct: 155 SGGIRPCVVAFGADQF-DESDPNQTTKTWNYFNWYYFCMGAAVLLAVTVLVWIQDNVGWG 214

Query: 129 TGFAVPAVLMLFSVLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRL------SLPY 188
            G  +P V M  SV+ F+ G  LY  + P  S  T L+QV V AF+ R+L      SL Y
Sbjct: 215 LGLGIPTVAMFLSVIAFVGGFQLYRHLVPAGSPFTRLIQVGVAAFRKRKLRMVSDPSLLY 274

Query: 189 SNYD---QYYLGPDPKFLVPTDSMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVES 248
            N +      LG     L  T  M  L+KA ++ + E++L P G I N W L +V  VE 
Sbjct: 275 FNDEIDAPISLGGK---LTHTKHMSFLDKAAIVTE-EDNLKP-GQIPNHWRLSTVHRVEE 334

Query: 249 LKNFLRIIPMWSTGILMLVC-LNQNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSV 308
           LK+ +R+ P+ ++GIL++     Q +F   QAKTMNRH+T SF+IPAGS+ V   +++  
Sbjct: 335 LKSVIRMGPIGASGILLITAYAQQGTFSLQQAKTMNRHLTNSFQIPAGSMSVFTTVAMLT 394

Query: 309 WLAFYDRIMVPLLER-CGHAGGLSPRLRMGIGLLLSCFAMAISAVVEGTRRRIATE-GLE 368
            + FYDR+ V +  +  G   G++   RMGIG ++S  A  ++  VE  R+ +A E GL 
Sbjct: 395 TIIFYDRVFVKVARKFTGLERGITFLHRMGIGFVISIIATLVAGFVEVKRKSVAIEHGLL 454

Query: 369 DQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVAS 428
           D+P+ ++ +S LW++PQ  + GVAE F +IG +E+FY Q P+++ S A AL+ + +++ +
Sbjct: 455 DKPHTIVPISFLWLIPQYGLHGVAEAFMSIGHLEFFYDQAPESMRSTATALFWMAISIGN 514

Query: 429 LVGSALVQMVNGITGKGNETSWL-DNNLNKGHLDYFYWLCAALALMNFVGYLLCNRTY 472
            V + LV +V+  + K + ++WL DNNLN+G L+YFYWL   L  +N V YL C + Y
Sbjct: 515 YVSTLLVTLVHKFSAKPDGSNWLPDNNLNRGRLEYFYWLITVLQAVNLVYYLWCAKIY 565

BLAST of MC08g_new0028 vs. ExPASy Swiss-Prot
Match: Q9LV10 (Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana OX=3702 GN=NPF2.11 PE=1 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 2.7e-83
Identity = 177/483 (36.65%), Postives = 283/483 (58.59%), Query Frame = 0

Query: 20  GMALLWLTAMIPQLKPSSC-SSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFG 79
           G  ++ LTA +PQL P++C +++  +C+  +  Q+A L   LGF+ +GAG IRPC++AFG
Sbjct: 128 GSFVILLTAAVPQLHPAACGTAADSICNGPSGGQIAFLLMGLGFLVVGAGGIRPCNLAFG 187

Query: 80  ADQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLF 139
           ADQ  N K  + +  +D++FNWY+ +   A +++LTL+VY+Q    W  G  +PAVLM  
Sbjct: 188 ADQF-NPKSESGKRGIDSFFNWYFFTFTFAQILSLTLVVYVQSNVSWTIGLTIPAVLMFL 247

Query: 140 SVLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRL---SLPYSNYDQYYLGPDPKF- 199
           + LIF  G  LYVK+K + S L G+ QVI VA K R L     P+ N   YY    PK+ 
Sbjct: 248 ACLIFFAGDKLYVKIKASGSPLAGIAQVIAVAIKKRGLKPAKQPWLNLYNYY---PPKYA 307

Query: 200 ---LVPTDSMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMW-ST 259
              L  TD  R L+KA ++   E+ L PDG  ++PW LC+++ VE +K  +R++P+W ++
Sbjct: 308 NSKLKYTDQFRFLDKAAIL-TPEDKLQPDGKPADPWKLCTMQQVEEVKCIVRVLPIWFAS 367

Query: 260 GILMLVCLNQNSFGTLQAKTMNRHI-TGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLL 319
            I  L    Q ++   QA   +R + +G F IPA +  V ++  ++V++  YDR++VP +
Sbjct: 368 SIYYLTITQQMTYPVFQALQSDRRLGSGGFVIPAATYVVFLMTGMTVFIVVYDRVLVPTM 427

Query: 320 ER-CGHAGGLSPRLRMGIGLLLSCFAMAISAVVEGTRRRIA----TEGLEDQPYAVIDMS 379
            R  G   G++   R+G G+  +  ++ ++  VE  RR  A    T G+  +   +  MS
Sbjct: 428 RRITGLDTGITLLQRIGTGIFFATASLVVAGFVEERRRTFALTKPTLGMAPRKGEISSMS 487

Query: 380 VLWIVPQCVVLGVAEGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMV 439
            +W++PQ  + GVAE F AIGQ+E++Y Q P+ + S A +++ V   V+S +GS L+  V
Sbjct: 488 AMWLIPQLSLAGVAEAFAAIGQMEFYYKQFPENMRSFAGSIFYVGGGVSSYLGSFLIATV 547

Query: 440 NGITGKGNETSWLDNNLNKGHLDYFYWLCAALALMNFVGYLLCNRTY---GLSENRRCNE 485
           +  T   +  +WL  +LNKG LD FY++ A +  +NF  +L+ +R Y   G  +     E
Sbjct: 548 HRTTQNSSGGNWLAEDLNKGRLDLFYFMIAGILAVNFAYFLVMSRWYRYKGSDDEVTTYE 605

BLAST of MC08g_new0028 vs. NCBI nr
Match: XP_022132098.1 (protein NRT1/ PTR FAMILY 1.2-like isoform X2 [Momordica charantia])

HSP 1 Score: 989 bits (2558), Expect = 0.0
Identity = 496/497 (99.80%), Postives = 496/497 (99.80%), Query Frame = 0

Query: 1   MLWPLLELFWLILTWGVSAGMALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSL 60
           MLWPLLELFWLILTWGVSAGMALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSL
Sbjct: 1   MLWPLLELFWLILTWGVSAGMALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSL 60

Query: 61  GFISIGAGCIRPCSIAFGADQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQ 120
           GFISIGAGCIRPCSIAFGADQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQ
Sbjct: 61  GFISIGAGCIRPCSIAFGADQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQ 120

Query: 121 DEFGWRTGFAVPAVLMLFSVLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPY 180
           DEFGWRTGFAVPAVLMLFSVLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPY
Sbjct: 121 DEFGWRTGFAVPAVLMLFSVLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPY 180

Query: 181 SNYDQYYLGPDPKFLVPTDSMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKN 240
           SNYDQYYLGPDPKFLVPTDSMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKN
Sbjct: 181 SNYDQYYLGPDPKFLVPTDSMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKN 240

Query: 241 FLRIIPMWSTGILMLVCLNQNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAF 300
           FLRIIPMWSTGILMLVCLNQNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAF
Sbjct: 241 FLRIIPMWSTGILMLVCLNQNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAF 300

Query: 301 YDRIMVPLLERCGHAGGLSPRLRMGIGLLLSCFAMAISAVVEGTRRRIATE-GLEDQPYA 360
           YDRIMVPLLERCGHAGGLSPRLRMGIGLLLSCFAMAISAVVEGTRRRIATE GLEDQPYA
Sbjct: 301 YDRIMVPLLERCGHAGGLSPRLRMGIGLLLSCFAMAISAVVEGTRRRIATEEGLEDQPYA 360

Query: 361 VIDMSVLWIVPQCVVLGVAEGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSA 420
           VIDMSVLWIVPQCVVLGVAEGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSA
Sbjct: 361 VIDMSVLWIVPQCVVLGVAEGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSA 420

Query: 421 LVQMVNGITGKGNETSWLDNNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRRC 480
           LVQMVNGITGKGNETSWLDNNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRRC
Sbjct: 421 LVQMVNGITGKGNETSWLDNNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRRC 480

Query: 481 NESNEVQDFEYRKLPSS 496
           NESNEVQDFEYRKLPSS
Sbjct: 481 NESNEVQDFEYRKLPSS 497

BLAST of MC08g_new0028 vs. NCBI nr
Match: XP_022132097.1 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 [Momordica charantia])

HSP 1 Score: 952 bits (2460), Expect = 0.0
Identity = 477/478 (99.79%), Postives = 477/478 (99.79%), Query Frame = 0

Query: 20  GMALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGA 79
           GMALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGA
Sbjct: 112 GMALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGA 171

Query: 80  DQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFS 139
           DQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFS
Sbjct: 172 DQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFS 231

Query: 140 VLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTD 199
           VLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTD
Sbjct: 232 VLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTD 291

Query: 200 SMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLN 259
           SMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLN
Sbjct: 292 SMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLN 351

Query: 260 QNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLS 319
           QNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLS
Sbjct: 352 QNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLS 411

Query: 320 PRLRMGIGLLLSCFAMAISAVVEGTRRRIATE-GLEDQPYAVIDMSVLWIVPQCVVLGVA 379
           PRLRMGIGLLLSCFAMAISAVVEGTRRRIATE GLEDQPYAVIDMSVLWIVPQCVVLGVA
Sbjct: 412 PRLRMGIGLLLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVA 471

Query: 380 EGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLD 439
           EGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLD
Sbjct: 472 EGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLD 531

Query: 440 NNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFEYRKLPSS 496
           NNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFEYRKLPSS
Sbjct: 532 NNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFEYRKLPSS 589

BLAST of MC08g_new0028 vs. NCBI nr
Match: XP_022131895.1 (protein NRT1/ PTR FAMILY 1.2-like [Momordica charantia])

HSP 1 Score: 802 bits (2072), Expect = 2.65e-288
Identity = 393/478 (82.22%), Postives = 435/478 (91.00%), Query Frame = 0

Query: 20  GMALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGA 79
           GMALLWLTA IPQLKPSSCSSSGILCDSAN YQLA+LFTSLGFISIGAGCI+PCS+AFGA
Sbjct: 112 GMALLWLTATIPQLKPSSCSSSGILCDSANLYQLAVLFTSLGFISIGAGCIKPCSMAFGA 171

Query: 80  DQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFS 139
           DQLTNEK  +N+GVLDTYFNWYYA+SGI++VI +T+IVYIQDEFGW TG+ VPAVLML S
Sbjct: 172 DQLTNEKNSSNKGVLDTYFNWYYAASGISTVIGMTVIVYIQDEFGWGTGYVVPAVLMLLS 231

Query: 140 VLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTD 199
            LIFL GSSLYVKVKP QSL TG+ QVIVVAFKNRRLSLPYSN+DQYYLG DPK LVPTD
Sbjct: 232 ALIFLAGSSLYVKVKPAQSLFTGIFQVIVVAFKNRRLSLPYSNFDQYYLGHDPKLLVPTD 291

Query: 200 SMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLN 259
           SMRCLNKACLIKDAENDLNPDGS+SNPWNLCSV+ VESLKNF+RIIP+WSTGI+M+VC+N
Sbjct: 292 SMRCLNKACLIKDAENDLNPDGSVSNPWNLCSVDQVESLKNFIRIIPVWSTGIIMVVCIN 351

Query: 260 QNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLS 319
           QN+ G LQAK MNRHITGSFE PAGSI V MIISLS+WL FYDRI++PLL+RCGHAG LS
Sbjct: 352 QNALGALQAKIMNRHITGSFEFPAGSINVFMIISLSLWLVFYDRIIMPLLKRCGHAGRLS 411

Query: 320 PRLRMGIGLLLSCFAMAISAVVEGTRRRIATE-GLEDQPYAVIDMSVLWIVPQCVVLGVA 379
           P LR+GIGLLLSCFAMAI+AVVE TRRRIA E GLEDQP AV+DMS LW+VPQCVVLGVA
Sbjct: 412 PELRIGIGLLLSCFAMAIAAVVEATRRRIAIEEGLEDQPNAVVDMSALWLVPQCVVLGVA 471

Query: 380 EGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLD 439
           EGFNA+GQIEYF+S++ K  SS+AVAL+TVEM+VA+LVGS LVQ+VNGITGKGNETSWLD
Sbjct: 472 EGFNAVGQIEYFFSKISKVSSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNETSWLD 531

Query: 440 NNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFEYRKLPSS 496
           NNLNKGHLDYFYWLCAALAL+N  GYLL NR YG SE  + NES+E+QDFEYR LPSS
Sbjct: 532 NNLNKGHLDYFYWLCAALALVNLCGYLLYNRAYGFSEKIQGNESSEIQDFEYRNLPSS 589

BLAST of MC08g_new0028 vs. NCBI nr
Match: XP_022962309.1 (protein NRT1/ PTR FAMILY 1.2-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 759 bits (1960), Expect = 5.25e-273
Identity = 378/477 (79.25%), Postives = 427/477 (89.52%), Query Frame = 0

Query: 21  MALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGAD 80
           M LLWLTA+IPQL+P+SCS SGILCDSANPYQLA+LF+SL FISIGAGCIRPCS+AFGAD
Sbjct: 1   MILLWLTAIIPQLRPASCSPSGILCDSANPYQLAVLFSSLVFISIGAGCIRPCSVAFGAD 60

Query: 81  QLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFSV 140
           QLT E+KPNNE VLD+YFNWYYAS GIA++IAL+LIV+IQD+FGW  GFAVPAVLMLFSV
Sbjct: 61  QLTKEEKPNNESVLDSYFNWYYASIGIATIIALSLIVFIQDKFGWGIGFAVPAVLMLFSV 120

Query: 141 LIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTDS 200
           LIFLVGSSLYVKVKPTQSLLTG L+VIVVAFKNR+LSLP+SN+DQYYLG DPK  +PT+S
Sbjct: 121 LIFLVGSSLYVKVKPTQSLLTGFLRVIVVAFKNRKLSLPFSNFDQYYLGRDPKCRIPTNS 180

Query: 201 MRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLNQ 260
           MR LNKACLIKDA+N+LNPDGS+SNPWNLCSV+ VESLK FLRIIPMWSTGILMLVCLNQ
Sbjct: 181 MRYLNKACLIKDAKNNLNPDGSVSNPWNLCSVDQVESLKAFLRIIPMWSTGILMLVCLNQ 240

Query: 261 NSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLSP 320
           NSFGT+QAKTMNRHIT SFEIPAGSI + M++SL  W+ FYDRI+VPLL R GHAGGLSP
Sbjct: 241 NSFGTIQAKTMNRHITRSFEIPAGSITIFMVLSLFSWIMFYDRILVPLLARHGHAGGLSP 300

Query: 321 RLRMGIGLLLSCFAMAISAVVEGTRRRIATE-GLEDQPYAVIDMSVLWIVPQCVVLGVAE 380
           +LR+GIGLLLSCFAMAISA V   RRR+A E G EDQP AVI+MS LW+VPQCVVLGVAE
Sbjct: 301 KLRIGIGLLLSCFAMAISAGVACIRRRVAIEQGFEDQPNAVINMSALWMVPQCVVLGVAE 360

Query: 381 GFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLDN 440
             N++GQ+EYFYSQMPKTLSSVAVALY+ EMAVA+LVGS +VQMVN ITG+GN+TSWLDN
Sbjct: 361 ALNSVGQVEYFYSQMPKTLSSVAVALYSFEMAVANLVGSIIVQMVNSITGEGNKTSWLDN 420

Query: 441 NLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFEYRKLPSS 496
           N+NKGHLDYFYWLCAALAL+NF GYL+    Y  +  R+  E NE++DFEYR LPSS
Sbjct: 421 NVNKGHLDYFYWLCAALALINFFGYLV----YCWALGRQDKEPNEMEDFEYRNLPSS 473

BLAST of MC08g_new0028 vs. NCBI nr
Match: XP_023545603.1 (protein NRT1/ PTR FAMILY 1.2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 763 bits (1970), Expect = 7.49e-273
Identity = 378/478 (79.08%), Postives = 427/478 (89.33%), Query Frame = 0

Query: 20  GMALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGA 79
           GM LLWLTA+IPQL+P+SCS SGILCDSANPYQLA+LF+SL FISIGAGCIRPCS+AFGA
Sbjct: 112 GMILLWLTAIIPQLRPASCSPSGILCDSANPYQLAVLFSSLVFISIGAGCIRPCSVAFGA 171

Query: 80  DQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFS 139
           DQLT E+KPNN+ VLD+YFNWYYAS GIA++IAL+LIV+IQD+FGW  GFAVPAVLMLFS
Sbjct: 172 DQLTKEEKPNNDSVLDSYFNWYYASIGIATIIALSLIVFIQDQFGWGIGFAVPAVLMLFS 231

Query: 140 VLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTD 199
           VLIFLVGSSLYVKVKPTQSLLTG L+VIVVAFKNR+LSLP+SN+DQYYLG DPK  +PTD
Sbjct: 232 VLIFLVGSSLYVKVKPTQSLLTGFLRVIVVAFKNRKLSLPFSNFDQYYLGRDPKCPIPTD 291

Query: 200 SMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLN 259
           SMRCLNKACLIKD +N+LNPDGS+SNPWNLCSV+ VESLK FLRIIPMWSTGILMLVCLN
Sbjct: 292 SMRCLNKACLIKDGKNNLNPDGSVSNPWNLCSVDQVESLKAFLRIIPMWSTGILMLVCLN 351

Query: 260 QNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLS 319
           QNSFGT+QAKTMNRHIT +FEIPAGSI + M++SL  W+ FYDRI+VPLL R GHAGGLS
Sbjct: 352 QNSFGTIQAKTMNRHITRTFEIPAGSITIFMVLSLFSWIMFYDRILVPLLARHGHAGGLS 411

Query: 320 PRLRMGIGLLLSCFAMAISAVVEGTRRRIATE-GLEDQPYAVIDMSVLWIVPQCVVLGVA 379
           P+LR+GIGLLLS FAMAISA V   RRR+A E G EDQP AVI+MS LW+VPQCVVLGVA
Sbjct: 412 PKLRIGIGLLLSFFAMAISAGVAYIRRRVAIEQGFEDQPNAVINMSALWMVPQCVVLGVA 471

Query: 380 EGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLD 439
           E  N++GQ+EYFYSQMPKTLSSVAVALY+ EMAVA+LVGS +VQMVN ITG+GN+TSWLD
Sbjct: 472 EALNSVGQVEYFYSQMPKTLSSVAVALYSFEMAVANLVGSIIVQMVNSITGEGNKTSWLD 531

Query: 440 NNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFEYRKLPSS 496
           NN+NKGHLDYFYWLCAALAL+NF GYL+C    G    R+  E NE++DFEYR LPSS
Sbjct: 532 NNVNKGHLDYFYWLCAALALINFFGYLVCCWALG----RQHKEPNEMEDFEYRNLPSS 585

BLAST of MC08g_new0028 vs. ExPASy TrEMBL
Match: A0A6J1BVB4 (protein NRT1/ PTR FAMILY 1.2-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111005055 PE=3 SV=1)

HSP 1 Score: 989 bits (2558), Expect = 0.0
Identity = 496/497 (99.80%), Postives = 496/497 (99.80%), Query Frame = 0

Query: 1   MLWPLLELFWLILTWGVSAGMALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSL 60
           MLWPLLELFWLILTWGVSAGMALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSL
Sbjct: 1   MLWPLLELFWLILTWGVSAGMALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSL 60

Query: 61  GFISIGAGCIRPCSIAFGADQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQ 120
           GFISIGAGCIRPCSIAFGADQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQ
Sbjct: 61  GFISIGAGCIRPCSIAFGADQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQ 120

Query: 121 DEFGWRTGFAVPAVLMLFSVLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPY 180
           DEFGWRTGFAVPAVLMLFSVLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPY
Sbjct: 121 DEFGWRTGFAVPAVLMLFSVLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPY 180

Query: 181 SNYDQYYLGPDPKFLVPTDSMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKN 240
           SNYDQYYLGPDPKFLVPTDSMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKN
Sbjct: 181 SNYDQYYLGPDPKFLVPTDSMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKN 240

Query: 241 FLRIIPMWSTGILMLVCLNQNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAF 300
           FLRIIPMWSTGILMLVCLNQNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAF
Sbjct: 241 FLRIIPMWSTGILMLVCLNQNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAF 300

Query: 301 YDRIMVPLLERCGHAGGLSPRLRMGIGLLLSCFAMAISAVVEGTRRRIATE-GLEDQPYA 360
           YDRIMVPLLERCGHAGGLSPRLRMGIGLLLSCFAMAISAVVEGTRRRIATE GLEDQPYA
Sbjct: 301 YDRIMVPLLERCGHAGGLSPRLRMGIGLLLSCFAMAISAVVEGTRRRIATEEGLEDQPYA 360

Query: 361 VIDMSVLWIVPQCVVLGVAEGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSA 420
           VIDMSVLWIVPQCVVLGVAEGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSA
Sbjct: 361 VIDMSVLWIVPQCVVLGVAEGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSA 420

Query: 421 LVQMVNGITGKGNETSWLDNNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRRC 480
           LVQMVNGITGKGNETSWLDNNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRRC
Sbjct: 421 LVQMVNGITGKGNETSWLDNNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRRC 480

Query: 481 NESNEVQDFEYRKLPSS 496
           NESNEVQDFEYRKLPSS
Sbjct: 481 NESNEVQDFEYRKLPSS 497

BLAST of MC08g_new0028 vs. ExPASy TrEMBL
Match: A0A6J1BSW7 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005055 PE=3 SV=1)

HSP 1 Score: 952 bits (2460), Expect = 0.0
Identity = 477/478 (99.79%), Postives = 477/478 (99.79%), Query Frame = 0

Query: 20  GMALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGA 79
           GMALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGA
Sbjct: 112 GMALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGA 171

Query: 80  DQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFS 139
           DQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFS
Sbjct: 172 DQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFS 231

Query: 140 VLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTD 199
           VLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTD
Sbjct: 232 VLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTD 291

Query: 200 SMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLN 259
           SMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLN
Sbjct: 292 SMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLN 351

Query: 260 QNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLS 319
           QNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLS
Sbjct: 352 QNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLS 411

Query: 320 PRLRMGIGLLLSCFAMAISAVVEGTRRRIATE-GLEDQPYAVIDMSVLWIVPQCVVLGVA 379
           PRLRMGIGLLLSCFAMAISAVVEGTRRRIATE GLEDQPYAVIDMSVLWIVPQCVVLGVA
Sbjct: 412 PRLRMGIGLLLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVA 471

Query: 380 EGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLD 439
           EGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLD
Sbjct: 472 EGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLD 531

Query: 440 NNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFEYRKLPSS 496
           NNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFEYRKLPSS
Sbjct: 532 NNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFEYRKLPSS 589

BLAST of MC08g_new0028 vs. ExPASy TrEMBL
Match: A0A6J1BQY7 (protein NRT1/ PTR FAMILY 1.2-like OS=Momordica charantia OX=3673 GN=LOC111004925 PE=3 SV=1)

HSP 1 Score: 802 bits (2072), Expect = 1.28e-288
Identity = 393/478 (82.22%), Postives = 435/478 (91.00%), Query Frame = 0

Query: 20  GMALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGA 79
           GMALLWLTA IPQLKPSSCSSSGILCDSAN YQLA+LFTSLGFISIGAGCI+PCS+AFGA
Sbjct: 112 GMALLWLTATIPQLKPSSCSSSGILCDSANLYQLAVLFTSLGFISIGAGCIKPCSMAFGA 171

Query: 80  DQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFS 139
           DQLTNEK  +N+GVLDTYFNWYYA+SGI++VI +T+IVYIQDEFGW TG+ VPAVLML S
Sbjct: 172 DQLTNEKNSSNKGVLDTYFNWYYAASGISTVIGMTVIVYIQDEFGWGTGYVVPAVLMLLS 231

Query: 140 VLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTD 199
            LIFL GSSLYVKVKP QSL TG+ QVIVVAFKNRRLSLPYSN+DQYYLG DPK LVPTD
Sbjct: 232 ALIFLAGSSLYVKVKPAQSLFTGIFQVIVVAFKNRRLSLPYSNFDQYYLGHDPKLLVPTD 291

Query: 200 SMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLN 259
           SMRCLNKACLIKDAENDLNPDGS+SNPWNLCSV+ VESLKNF+RIIP+WSTGI+M+VC+N
Sbjct: 292 SMRCLNKACLIKDAENDLNPDGSVSNPWNLCSVDQVESLKNFIRIIPVWSTGIIMVVCIN 351

Query: 260 QNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLS 319
           QN+ G LQAK MNRHITGSFE PAGSI V MIISLS+WL FYDRI++PLL+RCGHAG LS
Sbjct: 352 QNALGALQAKIMNRHITGSFEFPAGSINVFMIISLSLWLVFYDRIIMPLLKRCGHAGRLS 411

Query: 320 PRLRMGIGLLLSCFAMAISAVVEGTRRRIATE-GLEDQPYAVIDMSVLWIVPQCVVLGVA 379
           P LR+GIGLLLSCFAMAI+AVVE TRRRIA E GLEDQP AV+DMS LW+VPQCVVLGVA
Sbjct: 412 PELRIGIGLLLSCFAMAIAAVVEATRRRIAIEEGLEDQPNAVVDMSALWLVPQCVVLGVA 471

Query: 380 EGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLD 439
           EGFNA+GQIEYF+S++ K  SS+AVAL+TVEM+VA+LVGS LVQ+VNGITGKGNETSWLD
Sbjct: 472 EGFNAVGQIEYFFSKISKVSSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNETSWLD 531

Query: 440 NNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFEYRKLPSS 496
           NNLNKGHLDYFYWLCAALAL+N  GYLL NR YG SE  + NES+E+QDFEYR LPSS
Sbjct: 532 NNLNKGHLDYFYWLCAALALVNLCGYLLYNRAYGFSEKIQGNESSEIQDFEYRNLPSS 589

BLAST of MC08g_new0028 vs. ExPASy TrEMBL
Match: A0A6J1HGQ1 (protein NRT1/ PTR FAMILY 1.2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462798 PE=3 SV=1)

HSP 1 Score: 759 bits (1960), Expect = 2.54e-273
Identity = 378/477 (79.25%), Postives = 427/477 (89.52%), Query Frame = 0

Query: 21  MALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGAD 80
           M LLWLTA+IPQL+P+SCS SGILCDSANPYQLA+LF+SL FISIGAGCIRPCS+AFGAD
Sbjct: 1   MILLWLTAIIPQLRPASCSPSGILCDSANPYQLAVLFSSLVFISIGAGCIRPCSVAFGAD 60

Query: 81  QLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFSV 140
           QLT E+KPNNE VLD+YFNWYYAS GIA++IAL+LIV+IQD+FGW  GFAVPAVLMLFSV
Sbjct: 61  QLTKEEKPNNESVLDSYFNWYYASIGIATIIALSLIVFIQDKFGWGIGFAVPAVLMLFSV 120

Query: 141 LIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTDS 200
           LIFLVGSSLYVKVKPTQSLLTG L+VIVVAFKNR+LSLP+SN+DQYYLG DPK  +PT+S
Sbjct: 121 LIFLVGSSLYVKVKPTQSLLTGFLRVIVVAFKNRKLSLPFSNFDQYYLGRDPKCRIPTNS 180

Query: 201 MRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLNQ 260
           MR LNKACLIKDA+N+LNPDGS+SNPWNLCSV+ VESLK FLRIIPMWSTGILMLVCLNQ
Sbjct: 181 MRYLNKACLIKDAKNNLNPDGSVSNPWNLCSVDQVESLKAFLRIIPMWSTGILMLVCLNQ 240

Query: 261 NSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLSP 320
           NSFGT+QAKTMNRHIT SFEIPAGSI + M++SL  W+ FYDRI+VPLL R GHAGGLSP
Sbjct: 241 NSFGTIQAKTMNRHITRSFEIPAGSITIFMVLSLFSWIMFYDRILVPLLARHGHAGGLSP 300

Query: 321 RLRMGIGLLLSCFAMAISAVVEGTRRRIATE-GLEDQPYAVIDMSVLWIVPQCVVLGVAE 380
           +LR+GIGLLLSCFAMAISA V   RRR+A E G EDQP AVI+MS LW+VPQCVVLGVAE
Sbjct: 301 KLRIGIGLLLSCFAMAISAGVACIRRRVAIEQGFEDQPNAVINMSALWMVPQCVVLGVAE 360

Query: 381 GFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLDN 440
             N++GQ+EYFYSQMPKTLSSVAVALY+ EMAVA+LVGS +VQMVN ITG+GN+TSWLDN
Sbjct: 361 ALNSVGQVEYFYSQMPKTLSSVAVALYSFEMAVANLVGSIIVQMVNSITGEGNKTSWLDN 420

Query: 441 NLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFEYRKLPSS 496
           N+NKGHLDYFYWLCAALAL+NF GYL+    Y  +  R+  E NE++DFEYR LPSS
Sbjct: 421 NVNKGHLDYFYWLCAALALINFFGYLV----YCWALGRQDKEPNEMEDFEYRNLPSS 473

BLAST of MC08g_new0028 vs. ExPASy TrEMBL
Match: A0A6J1K777 (protein NRT1/ PTR FAMILY 1.2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491783 PE=3 SV=1)

HSP 1 Score: 758 bits (1957), Expect = 5.60e-273
Identity = 377/477 (79.04%), Postives = 425/477 (89.10%), Query Frame = 0

Query: 21  MALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGAD 80
           M LLWLTA+IPQL+P+SCS SGILCDSANPYQLA+LF+SL FISIGAGCIRPCS+AFGAD
Sbjct: 1   MILLWLTAIIPQLRPASCSPSGILCDSANPYQLAVLFSSLVFISIGAGCIRPCSVAFGAD 60

Query: 81  QLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFSV 140
           QLT E+KPNNE VLD+YFNWYYAS GIA++ AL+LIV+IQD+FGW  GFAVPAVLMLFSV
Sbjct: 61  QLTKEEKPNNESVLDSYFNWYYASIGIATIFALSLIVFIQDKFGWGIGFAVPAVLMLFSV 120

Query: 141 LIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTDS 200
           LIFLVGSSLYVKVKPTQSLLTG L+VIVVAFKNR+LSLP+SN+DQYYLG DPK  +PT+S
Sbjct: 121 LIFLVGSSLYVKVKPTQSLLTGFLRVIVVAFKNRKLSLPFSNFDQYYLGCDPKCPIPTNS 180

Query: 201 MRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLNQ 260
           MR LNKACLIKDA+N+LNPDGS+SNPWNLCSV+ VESLK FLRIIPMWSTGILMLVCLNQ
Sbjct: 181 MRYLNKACLIKDAKNNLNPDGSVSNPWNLCSVDQVESLKAFLRIIPMWSTGILMLVCLNQ 240

Query: 261 NSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLSP 320
           NSFGT+QAKTMNRHIT SFEIPAGSI + M++SL  W+ FYDRI+VPLL R GHAGGLSP
Sbjct: 241 NSFGTIQAKTMNRHITRSFEIPAGSITIFMVLSLFSWIMFYDRILVPLLARYGHAGGLSP 300

Query: 321 RLRMGIGLLLSCFAMAISAVVEGTRRRIATE-GLEDQPYAVIDMSVLWIVPQCVVLGVAE 380
           +LR+GIGLLLSCFAMAISA V   RRR+A E G EDQP AVI+MS LW+VPQCVVLGVAE
Sbjct: 301 KLRIGIGLLLSCFAMAISAGVACIRRRVAIEQGFEDQPNAVINMSALWMVPQCVVLGVAE 360

Query: 381 GFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLDN 440
             N++GQ+EYFYSQMPK LSSVAVALY+ EMAVA+LVGS +VQMVN ITG+GN+TSWLDN
Sbjct: 361 ALNSVGQVEYFYSQMPKMLSSVAVALYSFEMAVANLVGSIIVQMVNSITGEGNKTSWLDN 420

Query: 441 NLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFEYRKLPSS 496
           N+NKGHLDYFYWLCAALAL+NF GYL+C    G    R+  E NE++DFEYR LPSS
Sbjct: 421 NVNKGHLDYFYWLCAALALINFFGYLVCCWALG----RQDKEPNEMEDFEYRNLPSS 473

BLAST of MC08g_new0028 vs. TAIR 10
Match: AT1G52190.1 (Major facilitator superfamily protein )

HSP 1 Score: 454.9 bits (1169), Expect = 8.2e-128
Identity = 229/480 (47.71%), Postives = 336/480 (70.00%), Query Frame = 0

Query: 20  GMALLWLTAMIPQLKPSSC--SSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAF 79
           GM LLWLTAM+PQ+KPS C  +++G  C S+   QLA+L+++   ISIG+G IRPCS+AF
Sbjct: 106 GMVLLWLTAMLPQVKPSPCDPTAAGSHCGSSTASQLALLYSAFALISIGSGGIRPCSLAF 165

Query: 80  GADQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLML 139
           GADQL N++ P NE VL+++F WYYASS +A +IA T IVYIQ+  GW+ GF VPAVLML
Sbjct: 166 GADQLDNKENPKNERVLESFFGWYYASSAVAVLIAFTGIVYIQEHLGWKIGFGVPAVLML 225

Query: 140 FSVLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLP--YSNYDQYYLGPDPKFL 199
            + L+F++ S LYV    T+SL TGL Q IV A+K R+LSLP  + ++D YY   D +  
Sbjct: 226 IAALLFILASPLYVTRGVTKSLFTGLAQAIVAAYKKRKLSLPDHHDSFDCYYHMKDSEIK 285

Query: 200 VPTDSMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILML 259
            P+  +R LNKACLI + E ++  DG   NPW LC+ + VE LK  +++IP+WSTGI+M 
Sbjct: 286 APSQKLRFLNKACLISNREEEIGSDGFALNPWRLCTTDKVEELKALIKVIPIWSTGIMMS 345

Query: 260 VCLNQNSFGTLQAKTMNRHIT---GSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERC 319
           +  +Q+SF  LQA +M+R ++    SF++PAGS G+  II+L++W+  YDR ++PL  + 
Sbjct: 346 INTSQSSFQLLQATSMDRRLSRHGSSFQVPAGSFGMFTIIALALWVILYDRAVIPLASKI 405

Query: 320 -GHAGGLSPRLRMGIGLLLSCFAMAISAVVEGTRRRIA-TEGLEDQPYAVIDMSVLWIVP 379
            G    LS +LRMG+GL +S  AMAISA+VE  RR+ A ++G  +   AV+D+S +W+VP
Sbjct: 406 RGRPFRLSVKLRMGLGLFMSFLAMAISAMVESFRRKKAISQGYANNSNAVVDISAMWLVP 465

Query: 380 QCVVLGVAEGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGK 439
           Q V+ G+AE   AIGQ E+FY++ PK++SS+A +L+ + MAVASL+ S ++  VN +T +
Sbjct: 466 QYVLHGLAEALTAIGQTEFFYTEFPKSMSSIAASLFGLGMAVASLLASVVLNAVNELTSR 525

Query: 440 GNETSWLDNNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYG-LSENRRCNESNEVQDFE 490
             + SW+ +N+NKGH +Y+YW+ A ++ +N + Y++C+ +YG L +  R    N V++ E
Sbjct: 526 NGKESWVSDNINKGHYNYYYWVLAIMSFINVIYYVICSWSYGPLVDQVRNGRVNGVREEE 585

BLAST of MC08g_new0028 vs. TAIR 10
Match: AT3G16180.1 (Major facilitator superfamily protein )

HSP 1 Score: 438.0 bits (1125), Expect = 1.0e-122
Identity = 215/460 (46.74%), Postives = 321/460 (69.78%), Query Frame = 0

Query: 20  GMALLWLTAMIPQLKPSSC-SSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFG 79
           GM +LWLTAM+PQ+KPS C +++G  C SA   QLA+L+T+   ISIG+G IRPCS+AFG
Sbjct: 108 GMVVLWLTAMLPQVKPSPCVATAGTNCSSATSSQLALLYTAFALISIGSGGIRPCSLAFG 167

Query: 80  ADQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLF 139
           ADQL N++ P NE VL+++F WYYASS +A +IA T+IVYIQD  GW+ GF +PA+LML 
Sbjct: 168 ADQLDNKENPKNERVLESFFGWYYASSSVAVLIAFTVIVYIQDHLGWKIGFGIPAILMLL 227

Query: 140 SVLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLP--YSNYDQYYLGPDPKFLV 199
           +  +F+  S LYVK   ++SL TGL QV+  A+  R L+LP  + + D YY   D +   
Sbjct: 228 AGFLFVFASPLYVKRDVSKSLFTGLAQVVAAAYVKRNLTLPDHHDSRDCYYRLKDSELKA 287

Query: 200 PTDSMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLV 259
           P+D +R LNKAC I + + DL  DG   N W LC+ + VE LK  +++IP+WSTGI+M +
Sbjct: 288 PSDKLRFLNKACAISNRDEDLGSDGLALNQWRLCTTDQVEKLKALVKVIPVWSTGIMMSI 347

Query: 260 CLNQNSFGTLQAKTMNRHIT--GSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERC-G 319
            ++QNSF  LQAK+M+R ++   +F+IPAGS G+  II+L  W+  YDR ++PL  +  G
Sbjct: 348 NVSQNSFQLLQAKSMDRRLSSNSTFQIPAGSFGMFTIIALISWVVLYDRAILPLASKIRG 407

Query: 320 HAGGLSPRLRMGIGLLLSCFAMAISAVVEGTRRRIA-TEGLEDQPYAVIDMSVLWIVPQC 379
               ++ ++RMG+GL +S  AMA+SA VE  RR+ A ++GL +   + + +S +W+VPQ 
Sbjct: 408 RPVRVNVKIRMGLGLFISFLAMAVSATVEHYRRKTAISQGLANDANSTVSISAMWLVPQY 467

Query: 380 VVLGVAEGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGN 439
           V+ G+AE    IGQ E+FY++ PK++SS+A +L+ + MAVA+++ S ++  V   + +GN
Sbjct: 468 VLHGLAEALTGIGQTEFFYTEFPKSMSSIAASLFGLGMAVANILASVILNAVKNSSKQGN 527

Query: 440 ETSWLDNNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYG 473
             SW+++N+NKGH DY+YW+ A L+ +N + Y++C+ +YG
Sbjct: 528 -VSWIEDNINKGHYDYYYWVLAILSFVNVIYYVVCSWSYG 566

BLAST of MC08g_new0028 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 343.2 bits (879), Expect = 3.5e-94
Identity = 190/483 (39.34%), Postives = 284/483 (58.80%), Query Frame = 0

Query: 20  GMALLWLTAMIPQLKPSSCSSSGIL-CDSANPYQLAILFTSLGFISIGAGCIRPCSIAFG 79
           G+  + LTA  PQL P+SC+S   L C   N  Q+ +L   L F+S+G+G IRPCSI FG
Sbjct: 136 GLITITLTASFPQLHPASCNSQDPLSCGGPNKLQIGVLLLGLCFLSVGSGGIRPCSIPFG 195

Query: 80  ADQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLF 139
            DQ     +   +GV  ++FNWYY +  +  +I  T++VYIQD+  W  GF++P  LM  
Sbjct: 196 VDQFDQRTEEGVKGVA-SFFNWYYMTFTVVLIITQTVVVYIQDQVSWIIGFSIPTGLMAL 255

Query: 140 SVLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSN------YDQYYLGPDP 199
           +V++F  G   YV VKP  S+ +G+ QVIV A K R+L LP  +      YD        
Sbjct: 256 AVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKRKLKLPAEDDGTVTYYDPAIKSSVL 315

Query: 200 KFLVPTDSMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGI 259
             L  ++  RCL+KA ++   E DL P+G  ++ W LCSV+ VE +K  +RI+P+WS GI
Sbjct: 316 SKLHRSNQFRCLDKAAVV--IEGDLTPEGPPADKWRLCSVQEVEEVKCLIRIVPIWSAGI 375

Query: 260 LMLVCL-NQNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLER 319
           + L  +  Q +F   QA  M+R++   FEIPAGS+ V  ++++ ++L FYDR+ VP + R
Sbjct: 376 ISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSLSVISLLTIGIFLPFYDRVFVPFMRR 435

Query: 320 -CGHAGGLSPRLRMGIGLLLSCFAMAISAVVEGTRR-RIATEGLEDQPYAVIDMSVLWIV 379
             GH  G++   R+G G++ + F+M ++ +VE  RR R    G    P  +  MSV W+ 
Sbjct: 436 ITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMRRIRSINAG---DPTGMTPMSVFWLS 495

Query: 380 PQCVVLGVAEGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITG 439
           PQ +++G+ E FN IGQIE+F SQ P+ + S+A +L+++  A +S + S LV +V+  +G
Sbjct: 496 PQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSLFSLSFAGSSYLSSFLVTVVHKFSG 555

Query: 440 KGNETSWLDNNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFE 493
             +   WL+ NLN G LDYFY+L A L ++N V +  C R Y      R      ++DFE
Sbjct: 556 GHDRPDWLNKNLNAGKLDYFYYLIAVLGVVNLVYFWYCARGY------RYKVGLPIEDFE 606

BLAST of MC08g_new0028 vs. TAIR 10
Match: AT1G68570.1 (Major facilitator superfamily protein )

HSP 1 Score: 321.6 bits (823), Expect = 1.1e-87
Identity = 191/478 (39.96%), Postives = 285/478 (59.62%), Query Frame = 0

Query: 9   FWLILTWGV--SAGMALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIG 68
           FW I    +    GM LL ++A+IP L+P  C    + C  A+  QL+IL+ +L   ++G
Sbjct: 95  FWTITFASIIYQIGMTLLTISAIIPTLRPPPCKGEEV-CVVADTAQLSILYVALLLGALG 154

Query: 69  AGCIRPCSIAFGADQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWR 128
           +G IRPC +AFGADQ  +E  PN       YFNWYY   G A ++A+T++V+IQD  GW 
Sbjct: 155 SGGIRPCVVAFGADQF-DESDPNQTTKTWNYFNWYYFCMGAAVLLAVTVLVWIQDNVGWG 214

Query: 129 TGFAVPAVLMLFSVLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRL------SLPY 188
            G  +P V M  SV+ F+ G  LY  + P  S  T L+QV V AF+ R+L      SL Y
Sbjct: 215 LGLGIPTVAMFLSVIAFVGGFQLYRHLVPAGSPFTRLIQVGVAAFRKRKLRMVSDPSLLY 274

Query: 189 SNYD---QYYLGPDPKFLVPTDSMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVES 248
            N +      LG     L  T  M  L+KA ++ + E++L P G I N W L +V  VE 
Sbjct: 275 FNDEIDAPISLGGK---LTHTKHMSFLDKAAIVTE-EDNLKP-GQIPNHWRLSTVHRVEE 334

Query: 249 LKNFLRIIPMWSTGILMLVC-LNQNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSV 308
           LK+ +R+ P+ ++GIL++     Q +F   QAKTMNRH+T SF+IPAGS+ V   +++  
Sbjct: 335 LKSVIRMGPIGASGILLITAYAQQGTFSLQQAKTMNRHLTNSFQIPAGSMSVFTTVAMLT 394

Query: 309 WLAFYDRIMVPLLER-CGHAGGLSPRLRMGIGLLLSCFAMAISAVVEGTRRRIATE-GLE 368
            + FYDR+ V +  +  G   G++   RMGIG ++S  A  ++  VE  R+ +A E GL 
Sbjct: 395 TIIFYDRVFVKVARKFTGLERGITFLHRMGIGFVISIIATLVAGFVEVKRKSVAIEHGLL 454

Query: 369 DQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVAS 428
           D+P+ ++ +S LW++PQ  + GVAE F +IG +E+FY Q P+++ S A AL+ + +++ +
Sbjct: 455 DKPHTIVPISFLWLIPQYGLHGVAEAFMSIGHLEFFYDQAPESMRSTATALFWMAISIGN 514

Query: 429 LVGSALVQMVNGITGKGNETSWL-DNNLNKGHLDYFYWLCAALALMNFVGYLLCNRTY 472
            V + LV +V+  + K + ++WL DNNLN+G L+YFYWL   L  +N V YL C + Y
Sbjct: 515 YVSTLLVTLVHKFSAKPDGSNWLPDNNLNRGRLEYFYWLITVLQAVNLVYYLWCAKIY 565

BLAST of MC08g_new0028 vs. TAIR 10
Match: AT5G62680.1 (Major facilitator superfamily protein )

HSP 1 Score: 310.8 bits (795), Expect = 1.9e-84
Identity = 177/483 (36.65%), Postives = 283/483 (58.59%), Query Frame = 0

Query: 20  GMALLWLTAMIPQLKPSSC-SSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFG 79
           G  ++ LTA +PQL P++C +++  +C+  +  Q+A L   LGF+ +GAG IRPC++AFG
Sbjct: 128 GSFVILLTAAVPQLHPAACGTAADSICNGPSGGQIAFLLMGLGFLVVGAGGIRPCNLAFG 187

Query: 80  ADQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLF 139
           ADQ  N K  + +  +D++FNWY+ +   A +++LTL+VY+Q    W  G  +PAVLM  
Sbjct: 188 ADQF-NPKSESGKRGIDSFFNWYFFTFTFAQILSLTLVVYVQSNVSWTIGLTIPAVLMFL 247

Query: 140 SVLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRL---SLPYSNYDQYYLGPDPKF- 199
           + LIF  G  LYVK+K + S L G+ QVI VA K R L     P+ N   YY    PK+ 
Sbjct: 248 ACLIFFAGDKLYVKIKASGSPLAGIAQVIAVAIKKRGLKPAKQPWLNLYNYY---PPKYA 307

Query: 200 ---LVPTDSMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMW-ST 259
              L  TD  R L+KA ++   E+ L PDG  ++PW LC+++ VE +K  +R++P+W ++
Sbjct: 308 NSKLKYTDQFRFLDKAAIL-TPEDKLQPDGKPADPWKLCTMQQVEEVKCIVRVLPIWFAS 367

Query: 260 GILMLVCLNQNSFGTLQAKTMNRHI-TGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLL 319
            I  L    Q ++   QA   +R + +G F IPA +  V ++  ++V++  YDR++VP +
Sbjct: 368 SIYYLTITQQMTYPVFQALQSDRRLGSGGFVIPAATYVVFLMTGMTVFIVVYDRVLVPTM 427

Query: 320 ER-CGHAGGLSPRLRMGIGLLLSCFAMAISAVVEGTRRRIA----TEGLEDQPYAVIDMS 379
            R  G   G++   R+G G+  +  ++ ++  VE  RR  A    T G+  +   +  MS
Sbjct: 428 RRITGLDTGITLLQRIGTGIFFATASLVVAGFVEERRRTFALTKPTLGMAPRKGEISSMS 487

Query: 380 VLWIVPQCVVLGVAEGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMV 439
            +W++PQ  + GVAE F AIGQ+E++Y Q P+ + S A +++ V   V+S +GS L+  V
Sbjct: 488 AMWLIPQLSLAGVAEAFAAIGQMEFYYKQFPENMRSFAGSIFYVGGGVSSYLGSFLIATV 547

Query: 440 NGITGKGNETSWLDNNLNKGHLDYFYWLCAALALMNFVGYLLCNRTY---GLSENRRCNE 485
           +  T   +  +WL  +LNKG LD FY++ A +  +NF  +L+ +R Y   G  +     E
Sbjct: 548 HRTTQNSSGGNWLAEDLNKGRLDLFYFMIAGILAVNFAYFLVMSRWYRYKGSDDEVTTYE 605

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M8171.2e-12647.71Protein NRT1/ PTR FAMILY 1.2 OS=Arabidopsis thaliana OX=3702 GN=NPF1.2 PE=1 SV=1[more]
Q8LPL21.5e-12146.74Protein NRT1/ PTR FAMILY 1.1 OS=Arabidopsis thaliana OX=3702 GN=NPF1.1 PE=1 SV=2[more]
Q8RX774.9e-9339.34Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV... [more]
Q9SX201.5e-8639.96Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1[more]
Q9LV102.7e-8336.65Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana OX=3702 GN=NPF2.11 PE=1 SV... [more]
Match NameE-valueIdentityDescription
XP_022132098.10.099.80protein NRT1/ PTR FAMILY 1.2-like isoform X2 [Momordica charantia][more]
XP_022132097.10.099.79protein NRT1/ PTR FAMILY 1.2-like isoform X1 [Momordica charantia][more]
XP_022131895.12.65e-28882.22protein NRT1/ PTR FAMILY 1.2-like [Momordica charantia][more]
XP_022962309.15.25e-27379.25protein NRT1/ PTR FAMILY 1.2-like isoform X2 [Cucurbita moschata][more]
XP_023545603.17.49e-27379.08protein NRT1/ PTR FAMILY 1.2-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1BVB40.099.80protein NRT1/ PTR FAMILY 1.2-like isoform X2 OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1BSW70.099.79protein NRT1/ PTR FAMILY 1.2-like isoform X1 OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1BQY71.28e-28882.22protein NRT1/ PTR FAMILY 1.2-like OS=Momordica charantia OX=3673 GN=LOC111004925... [more]
A0A6J1HGQ12.54e-27379.25protein NRT1/ PTR FAMILY 1.2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1K7775.60e-27379.04protein NRT1/ PTR FAMILY 1.2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT1G52190.18.2e-12847.71Major facilitator superfamily protein [more]
AT3G16180.11.0e-12246.74Major facilitator superfamily protein [more]
AT1G69870.13.5e-9439.34nitrate transporter 1.7 [more]
AT1G68570.11.1e-8739.96Major facilitator superfamily protein [more]
AT5G62680.11.9e-8436.65Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 19..437
e-value: 8.5E-79
score: 265.2
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 19..475
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 8..480
e-value: 5.7E-129
score: 432.7
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 51..474
NoneNo IPR availablePANTHERPTHR11654:SF529SUBFAMILY NOT NAMEDcoord: 19..475

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g_new0028.1MC08g_new0028.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity