MC08g2577 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g2577
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	thermospermine synthase ACAULIS5-like
Location	MC08: 34399432 .. 34401596 (-)
RNA-Seq Expression	MC08g2577
Synteny	MC08g2577

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTTCATCCCTCTGCTCTGCCCTTTCTGTTCTCTTTATCCCCAACGCCAACGCCACTGCTCTCCCTCTCCAACATGACCGCCAACTCTCTCAATGGTACGAGGAGCAACTCGAGGATGATCTCAAATGGTCCTTTGCTGTCAACAGGTCCATCCTTTCTCTCTATATGCCTTGCCTTTAACTGTAGGGTAGATAACACTAATTTTGAGCACAACCGATCTTCACATTCACTTCCTCTAGGATACTTCATGCCACGACCAGCGAGTTTCAAGATGTAGTTCTCTTAGATACCAAACGATTTGGAAAGGTTTGGTTTATATCACACATCTTCTTCCTTACCAATAACACTTGATTAGTCCAAACGAGTTTACTGACACCATTTCAGGCTCTATTGCTTGATGGGAAGCTGCAAAGTGCTGAAAAAGATGAATTCATTTACCATGAGTCCTTGGTGCATCCTGCTCTGTTACTGCATCATAGGTATGCTGCCTTCCTTTTTGGTCGTGGGTCTGCAATCTGCAGCAAATCCGATTTTGTTCTTATCTTCCCTCTCGTCTCTTTGGATGATTTCAGCCCCAAAACAGTATTTATAATGGGAGGTGGAGAAGGGTCCACAGCTAGAGAGACGCTAAAGCACAAAGATATTGAGAAAGTTGTTATGTGTGATATTGATAGGGTATGGTTCTCAAACTCTTAAGAAAGATGCACTTATTTGTAATTTGTATCAATATGACCGAAAAATTAATAAGTAAAGAAGTGTTGATGGAAGAAAGGGAGGAGAACAAACCAATTGGTGCAATTGTCTTTCATTTGTGTTGTTACTGATAACCTTAGAGGGAACAACTTTGTAGGAGGTTGTCAATTTCTGCAATGCCCATCTGACCGAAAACCAGGATGCATTTCAGGACGAGAAACTTCACATTATTTTTGACGATGCAAAGTGAGTAGTTTTCCCTTTGGTCTATTATCCCATAAGCATGATTTCGATTTCCTTTTAATGATAGAGTTTCAGTAGGCACATGCCATAGTCCGTTCCATCTGCCATTTGCTATTAGTGCAATGTGTGTTATTGAGCTTGTCAAAGCTATATTTCTTGAACCAATGCATATTTCCCAAAGGATACAAGTTCTGTAATATCCATATGTCCAGTCGTCTAACCTAACCCACTCATAAATGAAACAGGGCTGGATTGGAGGGACAACCAGAGAAGTTCGATGTAATCATTGGCGATCTAGCGGATCCACACGAAGGAGGACCATGCAATCATCTTTACACTAAATCCTTCTATGAGGGAGTGATTAAGCCCAAGCTGAATGATAATGGTATATTTGTTACTCAGGTACATTATATTCTCAGTCCTAAATCTTCTCAACGCTATAGCAACCTCTCTCTGTTTTGTTCTTTATTCTCCTCTGTAAAATGTAGGCTGGCCCTGCTGGTATGCTCTCCCACAAGGTCTTCTCATCAATATACAACACTGTAAAGCATGTCTTCAGATGTAAGAATTTTGTTAGTATAAGCCGTGATTCGTTTTATTATGCAATTCGACTGTATTTTATTGATGCGATCTTCTATATATAAATTGTATCAATATAAATATTGGTTGGCGTAACATGTTTTGTTTCATCTGCACAGATGTGATTGCATACACAGCTCATGTTCCATCCTATGCAGATTCATGTGGATGGGTTTTGGTAAGCAACTAGAAAGAATTTAATTCCTTGTAATTGCCTGAACTGAAGGGAAAAAGAGGAGAGATGAGACATTATTATNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTATTGTACAGGCTTGTGATCATCCTCTTAAACTGGATGTGGAGGATCTGAACAACAGGATACGTGAGAGAGTCCAGGGCGAGCTACGCTACTTGGATGGTGCATTCATCGTATCATCAACGGTCATAAACAAGACTATTCGTACAACGTAAGTTCTCTACTAGATTGAATGAGCATTGATTTTTTGAAAACAAAACGAAATAGTTATCAAAGAGCACCTTAGCTTCCCCGCTAGATTGTATCTTGAGCATCGAGTTTTGGATGGTTATTTGTGTGTTGTGGGGTGCAGGGTGAAGAATGAGACTCATGTGTTCACTGAGGAAGATGCAAGGTTCGCACATGGCCAAGGCCTC

mRNA sequence

ATGTTTTCATCCCTCTGCTCTGCCCTTTCTGTTCTCTTTATCCCCAACGCCAACGCCACTGCTCTCCCTCTCCAACATGACCGCCAACTCTCTCAATGGTACGAGGAGCAACTCGAGGATGATCTCAAATGGTCCTTTGCTGTCAACAGGATACTTCATGCCACGACCAGCGAGTTTCAAGATGTAGTTCTCTTAGATACCAAACGATTTGGAAAGGCTCTATTGCTTGATGGGAAGCTGCAAAGTGCTGAAAAAGATGAATTCATTTACCATGAGTCCTTGGTGCATCCTGCTCTGTTACTGCATCATAGCCCCAAAACAGTATTTATAATGGGAGGTGGAGAAGGGTCCACAGCTAGAGAGACGCTAAAGCACAAAGATATTGAGAAAGTTGTTATGTGTGATATTGATAGGGAGGTTGTCAATTTCTGCAATGCCCATCTGACCGAAAACCAGGATGCATTTCAGGACGAGAAACTTCACATTATTTTTGACGATGCAAAGGCTGGATTGGAGGGACAACCAGAGAAGTTCGATGTAATCATTGGCGATCTAGCGGATCCACACGAAGGAGGACCATGCAATCATCTTTACACTAAATCCTTCTATGAGGGAGTGATTAAGCCCAAGCTGAATGATAATGGTATATTTGTTACTCAGGCTGGCCCTGCTGGTATGCTCTCCCACAAGGTCTTCTCATCAATATACAACACTGTAAAGCATGTCTTCAGATATGTGATTGCATACACAGCTCATGTTCCATCCTATGCAGATTCATGTGGATGGGTTTTGGCTTGTGATCATCCTCTTAAACTGGATGTGGAGGATCTGAACAACAGGATACGTGAGAGAGTCCAGGGCGAGCTACGCTACTTGGATGGTGCATTCATCGTATCATCAACGGTCATAAACAAGACTATTCGTACAACGGTGAAGAATGAGACTCATGTGTTCACTGAGGAAGATGCAAGGTTCGCACATGGCCAAGGCCTC

Coding sequence (CDS)

ATGTTTTCATCCCTCTGCTCTGCCCTTTCTGTTCTCTTTATCCCCAACGCCAACGCCACTGCTCTCCCTCTCCAACATGACCGCCAACTCTCTCAATGGTACGAGGAGCAACTCGAGGATGATCTCAAATGGTCCTTTGCTGTCAACAGGATACTTCATGCCACGACCAGCGAGTTTCAAGATGTAGTTCTCTTAGATACCAAACGATTTGGAAAGGCTCTATTGCTTGATGGGAAGCTGCAAAGTGCTGAAAAAGATGAATTCATTTACCATGAGTCCTTGGTGCATCCTGCTCTGTTACTGCATCATAGCCCCAAAACAGTATTTATAATGGGAGGTGGAGAAGGGTCCACAGCTAGAGAGACGCTAAAGCACAAAGATATTGAGAAAGTTGTTATGTGTGATATTGATAGGGAGGTTGTCAATTTCTGCAATGCCCATCTGACCGAAAACCAGGATGCATTTCAGGACGAGAAACTTCACATTATTTTTGACGATGCAAAGGCTGGATTGGAGGGACAACCAGAGAAGTTCGATGTAATCATTGGCGATCTAGCGGATCCACACGAAGGAGGACCATGCAATCATCTTTACACTAAATCCTTCTATGAGGGAGTGATTAAGCCCAAGCTGAATGATAATGGTATATTTGTTACTCAGGCTGGCCCTGCTGGTATGCTCTCCCACAAGGTCTTCTCATCAATATACAACACTGTAAAGCATGTCTTCAGATATGTGATTGCATACACAGCTCATGTTCCATCCTATGCAGATTCATGTGGATGGGTTTTGGCTTGTGATCATCCTCTTAAACTGGATGTGGAGGATCTGAACAACAGGATACGTGAGAGAGTCCAGGGCGAGCTACGCTACTTGGATGGTGCATTCATCGTATCATCAACGGTCATAAACAAGACTATTCGTACAACGGTGAAGAATGAGACTCATGTGTTCACTGAGGAAGATGCAAGGTTCGCACATGGCCAAGGCCTC

Protein sequence

MFSSLCSALSVLFIPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQDVVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTARETLKHKDIEKVVMCDIDREVVNFCNAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDVIIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLACDHPLKLDVEDLNNRIRERVQGELRYLDGAFIVSSTVINKTIRTTVKNETHVFTEEDARFAHGQGL

Homology

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BLAST of MC08g2577 vs. ExPASy Swiss-Prot
Match: Q9S7X6 (Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 3.5e-119
Identity = 200/319 (62.70%), Postives = 251/319 (78.68%), Query Frame = 0


Query: 14  IPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQDVVLLDTKRFGKA 73
           I  A +    L  ++Q   WYEE ++DDLKWSFA+N +LH  TSE+QD+ LLDTKRFGK 
Sbjct: 16  IHKATSPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKV 75

Query: 74  LLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTARETLKHKDIEKVVM 133
           L++DGK+QSAE+DEFIYHE L+HPALL H +PKTVFIMGGGEGS ARE LKH  IEKVVM
Sbjct: 76  LVIDGKMQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVM 135

Query: 134 CDIDREVVNFCNAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDVIIGDLADPHEGGP 193
           CDID+EVV+FC   LT N DAF ++KL ++  DAKA LE + EKFD+I+GDLADP EGGP
Sbjct: 136 CDIDQEVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGP 195

Query: 194 CNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHK-VFSSIYNTVKHVFRYVIAYTAH 253
           C  LYTKSFY+ ++KPKL+ NGIFVTQAGPAG+ +HK VF+SIYNT+K VF+YV AYTAH
Sbjct: 196 CYQLYTKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAH 255

Query: 254 VPSYADSCGWVLACDHPLKLDVEDLNNRIRERVQGELRYLDGAFIVSSTVINKTIRTTVK 313
           VPS+AD+ GWV+A DH   ++V++++ RI ERV GEL YL+    VS+  +NKTI   ++
Sbjct: 256 VPSFADTWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLNKTISLALE 315

Query: 314 NETHVFTEEDARFAHGQGL 332
            ET V++EE+ARF HG G+
Sbjct: 316 KETEVYSEENARFIHGHGV 334

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BLAST of MC08g2577 vs. ExPASy Swiss-Prot
Match: Q975S5 (Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) OX=273063 GN=speE PE=3 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 1.9e-56
Identity = 116/279 (41.58%), Postives = 172/279 (61.65%), Query Frame = 0


Query: 47  AVNRILHATTSEFQDVVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPK 106
           A+ +++    + +Q  +L++  RFGKAL+LDGK+QS   DEFIYHE+LVHP LL  ++P+
Sbjct: 20  AITKVIAEEKTPYQRALLVELARFGKALILDGKIQSTITDEFIYHEALVHPLLLSINNPE 79

Query: 107 TVFIMGGGEGSTARETLKHKDIEKVVMCDIDREVVNFCNAHLTE-NQDAFQDEKLHIIFD 166
            + I+GGGEG+T RE LKHK I+ V M DID  V++F   +L E +Q AF + K  ++ +
Sbjct: 80  KILILGGGEGATLREVLKHKTIKNVTMVDIDPVVIDFAKKYLQEWHQGAFDNPKSKLVIE 139

Query: 167 DAKAGLEGQPEKFDVIIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAG 226
           D    ++   EKFD ++ DL DP +  P   LYTK FYE V   +++  GI      P+ 
Sbjct: 140 DGYKFIKETKEKFDAVVIDLTDPIKDSPSQMLYTKEFYEEV--KRISKWGIVTQATSPS- 199

Query: 227 MLSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLACD--HPLKLDVEDLNNRIRE 286
             S + FS IYNT+KHVF+ V A   +VP++    G+V A D  +P +   E++NNRI+E
Sbjct: 200 -FSLETFSIIYNTIKHVFKKVSAGITYVPAFDGLWGFVYASDEVNPAEFSKEEINNRIKE 259

Query: 287 RVQGELRYLDGAFIVSSTVINKTIRTTVKNETHVFTEED 323
           R+ G LR+ DG   ++   I K IR  ++ E  + T E+
Sbjct: 260 RIDGSLRFYDGETHITMFSIPKHIREVLEKENKISTREN 294

[Close]

BLAST of MC08g2577 vs. ExPASy Swiss-Prot
Match: Q72K55 (Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) OX=262724 GN=speE PE=3 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 4.6e-55
Identity = 117/287 (40.77%), Postives = 171/287 (59.58%), Query Frame = 0


Query: 48  VNRILHATTSEFQDVVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKT 107
           + R++ +  + FQD  L ++K FGK L+LD  +QS E+DE+IYHE+LVHPA+L H  PK 
Sbjct: 21  MERVIASGKTPFQDYFLFESKGFGKVLILDKDVQSTERDEYIYHETLVHPAMLTHPEPKR 80

Query: 108 VFIMGGGEGSTARETLKHKDIEKVVMCDIDREVVNFCNAHLTE-NQDAFQDEKLHIIFDD 167
           V I+GGGEG+T RE LKH  +EK VM DID E+V     H+ E +Q AF D +  ++ DD
Sbjct: 81  VLIVGGGEGATLREVLKHPTVEKAVMVDIDGELVEVAKRHMPEWHQGAFDDPRAVLVIDD 140

Query: 168 AKAGLEGQPEKFDVIIGDLADP-HEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAG 227
           A+A LE   E++DV+I DL DP  E  P   LYT  FY  ++K  LN  G+   QAG   
Sbjct: 141 ARAYLERTEERYDVVIIDLTDPVGEDNPARLLYTVEFYR-LVKAHLNPGGVMGMQAGMIL 200

Query: 228 MLSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLACD--HPLKLDVEDLNNRIRE 287
           +  H+V   ++ TV+  FRYV +Y  H+P +  + G++LA D   P       +  RIRE
Sbjct: 201 LTHHRVHPVVHRTVREAFRYVRSYKNHIPGFFLNFGFLLASDAFDPAAFSEGVIEARIRE 260

Query: 288 RVQGELRYLDGAFIVSSTVINKTIRTTVKNETHVFTEEDARFAHGQG 331
           R    LR+L   ++ +  V+ K +   ++ ET V T+++  +   +G
Sbjct: 261 R-NLALRHLTAPYLEAMFVLPKDLLEALEKETMVSTDQNPFYVTPEG 305

[Close]

BLAST of MC08g2577 vs. ExPASy Swiss-Prot
Match: Q5SK28 (Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) OX=300852 GN=speE PE=1 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 4.6e-55
Identity = 117/287 (40.77%), Postives = 171/287 (59.58%), Query Frame = 0


Query: 48  VNRILHATTSEFQDVVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKT 107
           + R++ +  + FQD  L ++K FGK L+LD  +QS E+DE+IYHE+LVHPA+L H  PK 
Sbjct: 21  MERVIASGKTPFQDYFLFESKGFGKVLILDKDVQSTERDEYIYHETLVHPAMLTHPEPKR 80

Query: 108 VFIMGGGEGSTARETLKHKDIEKVVMCDIDREVVNFCNAHLTE-NQDAFQDEKLHIIFDD 167
           V I+GGGEG+T RE LKH  +EK VM DID E+V     H+ E +Q AF D +  ++ DD
Sbjct: 81  VLIVGGGEGATLREVLKHPTVEKAVMVDIDGELVEVAKRHMPEWHQGAFDDPRAVLVIDD 140

Query: 168 AKAGLEGQPEKFDVIIGDLADP-HEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAG 227
           A+A LE   E++DV+I DL DP  E  P   LYT  FY  ++K  LN  G+   QAG   
Sbjct: 141 ARAYLERTEERYDVVIIDLTDPVGEDNPARLLYTVEFYR-LVKAHLNPGGVMGMQAGMIL 200

Query: 228 MLSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLACD--HPLKLDVEDLNNRIRE 287
           +  H+V   ++ TV+  FRYV +Y  H+P +  + G++LA D   P       +  RIRE
Sbjct: 201 LTHHRVHPVVHRTVREAFRYVRSYKNHIPGFFLNFGFLLASDAFDPAAFSEGVIEARIRE 260

Query: 288 RVQGELRYLDGAFIVSSTVINKTIRTTVKNETHVFTEEDARFAHGQG 331
           R    LR+L   ++ +  V+ K +   ++ ET V T+++  +   +G
Sbjct: 261 R-NLALRHLTAPYLEAMFVLPKDLLEALEKETMVSTDQNPFYVTPEG 305

[Close]

BLAST of MC08g2577 vs. ExPASy Swiss-Prot
Match: Q9YE02 (Polyamine aminopropyltransferase OS=Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) OX=272557 GN=speE PE=3 SV=2)

HSP 1 Score: 210.7 bits (535), Expect = 2.5e-53
Identity = 119/299 (39.80%), Postives = 173/299 (57.86%), Query Frame = 0


Query: 33  WYEEQLEDDLKWSFAVNRILHATTSEFQDVVLLDTKRFGKALLLDGKLQSAEKDEFIYHE 92
           W+ E L  D      +  ++    S FQ++ ++     GK L+LDGK QS+E DEF+YHE
Sbjct: 15  WFLEWLTPDRATLKHIEDVIFQGRSRFQEIAVVRVSGEGKVLVLDGKTQSSESDEFMYHE 74

Query: 93  SLVHPALLLHHSPKTVFIMGGGEGSTARETLKHKDIEKVVMCDIDREVVNFCNAHLTE-N 152
           +LVHPA++LH SP+ V I+GGGEG+T RE LKH+ +EK VM DID  VVN    HL E +
Sbjct: 75  ALVHPAMILHGSPRKVLILGGGEGATLREVLKHRSVEKAVMVDIDETVVNVAREHLREWH 134

Query: 153 QDAFQDEKLHIIFDDAKAGLEGQPEK-FDVIIGDLADPHEGGPCNHLYTKSFYEGVIKPK 212
           + AF D +  ++ DDA   +  + E  FDV+I DL DP E GP   LY++ +Y  ++K  
Sbjct: 135 RGAFDDPRAEVVIDDAWNYVASKAETGFDVVIADLVDPLEAGPATRLYSEEYYR-MVKDV 194

Query: 213 LNDNGIFVTQAGPAGMLSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLACDHPL 272
           +N  G+FVTQA     L+ +  + I NTV  VF++V +Y  +VPS+    G+V+A D   
Sbjct: 195 MNPGGVFVTQAVSISHLT-EYHAIIRNTVARVFKHVESYGVYVPSFDSMWGFVVASDDKD 254

Query: 273 KLDVED---LNNRIRERVQG-ELRYLDGAFIVSSTVINKTIRTTVKNETHVFTEEDARF 326
              + D      R+  ++QG ELR+LD A ++    I K  R  +  E    T ++  F
Sbjct: 255 PRILGDRGFFETRLSHQLQGAELRFLDYASMLHMLNIPKMYREAIAREKRYATLDNQVF 311

[Close]

BLAST of MC08g2577 vs. NCBI nr
Match: XP_038890496.1 (thermospermine synthase ACAULIS5-like [Benincasa hispida])

HSP 1 Score: 615 bits (1585), Expect = 4.17e-221
Identity = 295/331 (89.12%), Postives = 318/331 (96.07%), Query Frame = 0


Query: 1   MFSSLCSALSVLFIPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQ 60
           MFSS CSALSVLFIPN+  T+LPL H  Q+S+W+EEQLEDDLKWSFAVNRILHA TSEFQ
Sbjct: 1   MFSSFCSALSVLFIPNS--TSLPLDHIHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQ 60

Query: 61  DVVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTAR 120
           D+VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHH+PKTVFIMGGGEGSTAR
Sbjct: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTAR 120

Query: 121 ETLKHKDIEKVVMCDIDREVVNFCNAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDV 180
           ETLKHKDIEKVVMCDIDR+VVNFC AHL ENQDAFQDEKLHIIFDDAKAGLEG+PEKFDV
Sbjct: 121 ETLKHKDIEKVVMCDIDRDVVNFCRAHLKENQDAFQDEKLHIIFDDAKAGLEGRPEKFDV 180

Query: 181 IIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHKVFSSIYNTVK 240
           IIGDL+DPHEGGPCNHLYTKSFYE VIKPKLNDNGIFVTQAGPAG+LSH+VFSSIYNTVK
Sbjct: 181 IIGDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHEVFSSIYNTVK 240

Query: 241 HVFRYVIAYTAHVPSYADSCGWVLACDHPLKLDVEDLNNRIRERVQGELRYLDGAFIVSS 300
           HVF+YVIAYTAHVPSYADSCGWVLACDHP++LD+EDLNNRIRERV+GEL YLDGA+IVSS
Sbjct: 241 HVFKYVIAYTAHVPSYADSCGWVLACDHPIELDMEDLNNRIRERVRGELHYLDGAYIVSS 300

Query: 301 TVINKTIRTTVKNETHVFTEEDARFAHGQGL 331
           TV+NKTIR ++ NETH+FTEEDARFAHG+GL
Sbjct: 301 TVVNKTIRASMMNETHLFTEEDARFAHGRGL 329

[Close]

BLAST of MC08g2577 vs. NCBI nr
Match: XP_004141174.1 (thermospermine synthase ACAULIS5 [Cucumis sativus] >KGN60515.1 hypothetical protein Csa_019446 [Cucumis sativus])

HSP 1 Score: 612 bits (1579), Expect = 3.42e-220
Identity = 295/331 (89.12%), Postives = 316/331 (95.47%), Query Frame = 0


Query: 1   MFSSLCSALSVLFIPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQ 60
           MFSS CSALSVLF+PN+++  LPL H +QL QW+EE+LEDDLKWSFAVNR+LHATTSEFQ
Sbjct: 1   MFSSFCSALSVLFVPNSSS--LPLDHIQQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQ 60

Query: 61  DVVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTAR 120
           D+VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHH+PKTVFIMGGGEG TAR
Sbjct: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTAR 120

Query: 121 ETLKHKDIEKVVMCDIDREVVNFCNAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDV 180
           ETLKHK IEKVVMCDIDR+VVNFC AHL ENQDAFQDE+LHIIFDDAKAGLEG+PEKFDV
Sbjct: 121 ETLKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDERLHIIFDDAKAGLEGRPEKFDV 180

Query: 181 IIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHKVFSSIYNTVK 240
           IIGDL+DPHEGGPCNHLYTKSFYE VIKPKL+DNGIFVTQAGPAG+LSHKVFSSIYNTVK
Sbjct: 181 IIGDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVK 240

Query: 241 HVFRYVIAYTAHVPSYADSCGWVLACDHPLKLDVEDLNNRIRERVQGELRYLDGAFIVSS 300
           HVFRYVIAYTAHVPSYADSCGWVLA DHP+KLD+EDLNN+IRERVQGEL YLDGAFIVSS
Sbjct: 241 HVFRYVIAYTAHVPSYADSCGWVLASDHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSS 300

Query: 301 TVINKTIRTTVKNETHVFTEEDARFAHGQGL 331
           TVINKTIRT + NETHVFTEEDARFAHG+GL
Sbjct: 301 TVINKTIRTLMMNETHVFTEEDARFAHGRGL 329

[Close]

BLAST of MC08g2577 vs. NCBI nr
Match: XP_008459675.1 (PREDICTED: thermospermine synthase ACAULIS5-like [Cucumis melo] >KAA0061473.1 thermospermine synthase ACAULIS5-like [Cucumis melo var. makuwa] >TYK10801.1 thermospermine synthase ACAULIS5-like [Cucumis melo var. makuwa])

HSP 1 Score: 607 bits (1564), Expect = 6.61e-218
Identity = 294/331 (88.82%), Postives = 314/331 (94.86%), Query Frame = 0


Query: 1   MFSSLCSALSVLFIPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQ 60
           MFSS CSALSVLFIPN+++  LPL H  QL QW+EE+LEDDLKWSFAVNR+LHATTSEFQ
Sbjct: 1   MFSSFCSALSVLFIPNSSS--LPLDHIHQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQ 60

Query: 61  DVVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTAR 120
           D+VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHH+PKTVFIMGGGEGSTAR
Sbjct: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTAR 120

Query: 121 ETLKHKDIEKVVMCDIDREVVNFCNAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDV 180
           ETLKHK IEKVVMCDIDR+VVNFC AHL ENQDAFQDEKLHIIFDDAKAGLEG+ EKFDV
Sbjct: 121 ETLKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDEKLHIIFDDAKAGLEGRAEKFDV 180

Query: 181 IIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHKVFSSIYNTVK 240
           IIGDL+DPHEGGPCNHLYTKSFYE VIKPKL+DNGIFVTQAGPAG+LSHKVFSSIYNTVK
Sbjct: 181 IIGDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVK 240

Query: 241 HVFRYVIAYTAHVPSYADSCGWVLACDHPLKLDVEDLNNRIRERVQGELRYLDGAFIVSS 300
           HVF+YVIAYTAHVPSYADSCGWVLA DHP++LD EDLNN+I ERV+GELRYLDGAFIVSS
Sbjct: 241 HVFKYVIAYTAHVPSYADSCGWVLASDHPIRLDKEDLNNKIHERVRGELRYLDGAFIVSS 300

Query: 301 TVINKTIRTTVKNETHVFTEEDARFAHGQGL 331
           TVINKTIRT + NETHVFTEEDARFAHG+GL
Sbjct: 301 TVINKTIRTLMMNETHVFTEEDARFAHGRGL 329

[Close]

BLAST of MC08g2577 vs. NCBI nr
Match: XP_022946737.1 (thermospermine synthase ACAULIS5-like [Cucurbita moschata])

HSP 1 Score: 582 bits (1500), Expect = 3.34e-208
Identity = 282/331 (85.20%), Postives = 304/331 (91.84%), Query Frame = 0


Query: 1   MFSSLCSALSVLFIPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQ 60
           M S LCSAL  L IP +N+TA+P      +S+W+EEQLEDDLKWSFAVNRILHA TS+FQ
Sbjct: 1   MLSPLCSALPALSIPTSNSTAVP-----HVSEWFEEQLEDDLKWSFAVNRILHAATSKFQ 60

Query: 61  DVVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTAR 120
           D+VLLDTKRFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHH+PKTVFI+GGGEGSTAR
Sbjct: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTAR 120

Query: 121 ETLKHKDIEKVVMCDIDREVVNFCNAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDV 180
           ETLKHK IEKVVMCDIDR+VVNFC AHL+ENQDAFQDEKLHIIFDDAK GLEGQPEKFDV
Sbjct: 121 ETLKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDV 180

Query: 181 IIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHKVFSSIYNTVK 240
           II DLADPHEGGPCNHLYTKSFYE  IKPKLNDNGIFVTQAGP+G+LS+ VFSSIYNTVK
Sbjct: 181 IISDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVK 240

Query: 241 HVFRYVIAYTAHVPSYADSCGWVLACDHPLKLDVEDLNNRIRERVQGELRYLDGAFIVSS 300
           HVF+YVIAYTAH+PSYADS GWVLA DHP KLD+EDL+ RIRERV+GELRYLDGAFIVSS
Sbjct: 241 HVFKYVIAYTAHIPSYADSSGWVLASDHPFKLDIEDLDKRIRERVRGELRYLDGAFIVSS 300

Query: 301 TVINKTIRTTVKNETHVFTEEDARFAHGQGL 331
           TVINKTIRT + NETHVFTEEDARF HGQGL
Sbjct: 301 TVINKTIRTAMMNETHVFTEEDARFTHGQGL 326

[Close]

BLAST of MC08g2577 vs. NCBI nr
Match: KAG7030499.1 (Thermospermine synthase ACAULIS5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 582 bits (1500), Expect = 3.34e-208
Identity = 282/331 (85.20%), Postives = 304/331 (91.84%), Query Frame = 0


Query: 1   MFSSLCSALSVLFIPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQ 60
           M S LCSAL  L IP +N+TA+P      +S+W+EEQLEDDLKWSFAVNRILHA TS+FQ
Sbjct: 1   MLSPLCSALPALSIPTSNSTAVP-----HVSEWFEEQLEDDLKWSFAVNRILHAATSKFQ 60

Query: 61  DVVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTAR 120
           D+VLLDTKRFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHH+PKTVFI+GGGEGSTAR
Sbjct: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTAR 120

Query: 121 ETLKHKDIEKVVMCDIDREVVNFCNAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDV 180
           ETLKHK IEKVVMCDIDR+VVNFC AHL+ENQDAFQDEKLHIIFDDAK GLEGQPEKFD+
Sbjct: 121 ETLKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDI 180

Query: 181 IIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHKVFSSIYNTVK 240
           II DLADPHEGGPCNHLYTKSFYE  IKPKLNDNGIFVTQAGP+G+LS+ VFSSIYNTVK
Sbjct: 181 IISDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVK 240

Query: 241 HVFRYVIAYTAHVPSYADSCGWVLACDHPLKLDVEDLNNRIRERVQGELRYLDGAFIVSS 300
           HVF+YVIAYTAH+PSYADS GWVLA DHP KLDVEDL+ RIRERV+GELRYLDGAFIVSS
Sbjct: 241 HVFKYVIAYTAHIPSYADSSGWVLASDHPFKLDVEDLDKRIRERVRGELRYLDGAFIVSS 300

Query: 301 TVINKTIRTTVKNETHVFTEEDARFAHGQGL 331
           TVINKTIRT + NETHVFTEEDARF HGQGL
Sbjct: 301 TVINKTIRTAMMNETHVFTEEDARFTHGQGL 326

[Close]

BLAST of MC08g2577 vs. ExPASy TrEMBL
Match: A0A0A0LET5 (PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000240 PE=3 SV=1)

HSP 1 Score: 612 bits (1579), Expect = 1.66e-220
Identity = 295/331 (89.12%), Postives = 316/331 (95.47%), Query Frame = 0


Query: 1   MFSSLCSALSVLFIPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQ 60
           MFSS CSALSVLF+PN+++  LPL H +QL QW+EE+LEDDLKWSFAVNR+LHATTSEFQ
Sbjct: 1   MFSSFCSALSVLFVPNSSS--LPLDHIQQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQ 60

Query: 61  DVVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTAR 120
           D+VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHH+PKTVFIMGGGEG TAR
Sbjct: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTAR 120

Query: 121 ETLKHKDIEKVVMCDIDREVVNFCNAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDV 180
           ETLKHK IEKVVMCDIDR+VVNFC AHL ENQDAFQDE+LHIIFDDAKAGLEG+PEKFDV
Sbjct: 121 ETLKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDERLHIIFDDAKAGLEGRPEKFDV 180

Query: 181 IIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHKVFSSIYNTVK 240
           IIGDL+DPHEGGPCNHLYTKSFYE VIKPKL+DNGIFVTQAGPAG+LSHKVFSSIYNTVK
Sbjct: 181 IIGDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVK 240

Query: 241 HVFRYVIAYTAHVPSYADSCGWVLACDHPLKLDVEDLNNRIRERVQGELRYLDGAFIVSS 300
           HVFRYVIAYTAHVPSYADSCGWVLA DHP+KLD+EDLNN+IRERVQGEL YLDGAFIVSS
Sbjct: 241 HVFRYVIAYTAHVPSYADSCGWVLASDHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSS 300

Query: 301 TVINKTIRTTVKNETHVFTEEDARFAHGQGL 331
           TVINKTIRT + NETHVFTEEDARFAHG+GL
Sbjct: 301 TVINKTIRTLMMNETHVFTEEDARFAHGRGL 329

[Close]

BLAST of MC08g2577 vs. ExPASy TrEMBL
Match: A0A5A7UZV1 (Thermospermine synthase ACAULIS5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G001170 PE=3 SV=1)

HSP 1 Score: 607 bits (1564), Expect = 3.20e-218
Identity = 294/331 (88.82%), Postives = 314/331 (94.86%), Query Frame = 0


Query: 1   MFSSLCSALSVLFIPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQ 60
           MFSS CSALSVLFIPN+++  LPL H  QL QW+EE+LEDDLKWSFAVNR+LHATTSEFQ
Sbjct: 1   MFSSFCSALSVLFIPNSSS--LPLDHIHQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQ 60

Query: 61  DVVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTAR 120
           D+VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHH+PKTVFIMGGGEGSTAR
Sbjct: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTAR 120

Query: 121 ETLKHKDIEKVVMCDIDREVVNFCNAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDV 180
           ETLKHK IEKVVMCDIDR+VVNFC AHL ENQDAFQDEKLHIIFDDAKAGLEG+ EKFDV
Sbjct: 121 ETLKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDEKLHIIFDDAKAGLEGRAEKFDV 180

Query: 181 IIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHKVFSSIYNTVK 240
           IIGDL+DPHEGGPCNHLYTKSFYE VIKPKL+DNGIFVTQAGPAG+LSHKVFSSIYNTVK
Sbjct: 181 IIGDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVK 240

Query: 241 HVFRYVIAYTAHVPSYADSCGWVLACDHPLKLDVEDLNNRIRERVQGELRYLDGAFIVSS 300
           HVF+YVIAYTAHVPSYADSCGWVLA DHP++LD EDLNN+I ERV+GELRYLDGAFIVSS
Sbjct: 241 HVFKYVIAYTAHVPSYADSCGWVLASDHPIRLDKEDLNNKIHERVRGELRYLDGAFIVSS 300

Query: 301 TVINKTIRTTVKNETHVFTEEDARFAHGQGL 331
           TVINKTIRT + NETHVFTEEDARFAHG+GL
Sbjct: 301 TVINKTIRTLMMNETHVFTEEDARFAHGRGL 329

[Close]

BLAST of MC08g2577 vs. ExPASy TrEMBL
Match: A0A1S3CAR3 (thermospermine synthase ACAULIS5-like OS=Cucumis melo OX=3656 GN=LOC103498720 PE=3 SV=1)

HSP 1 Score: 607 bits (1564), Expect = 3.20e-218
Identity = 294/331 (88.82%), Postives = 314/331 (94.86%), Query Frame = 0


Query: 1   MFSSLCSALSVLFIPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQ 60
           MFSS CSALSVLFIPN+++  LPL H  QL QW+EE+LEDDLKWSFAVNR+LHATTSEFQ
Sbjct: 1   MFSSFCSALSVLFIPNSSS--LPLDHIHQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQ 60

Query: 61  DVVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTAR 120
           D+VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHH+PKTVFIMGGGEGSTAR
Sbjct: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTAR 120

Query: 121 ETLKHKDIEKVVMCDIDREVVNFCNAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDV 180
           ETLKHK IEKVVMCDIDR+VVNFC AHL ENQDAFQDEKLHIIFDDAKAGLEG+ EKFDV
Sbjct: 121 ETLKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDEKLHIIFDDAKAGLEGRAEKFDV 180

Query: 181 IIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHKVFSSIYNTVK 240
           IIGDL+DPHEGGPCNHLYTKSFYE VIKPKL+DNGIFVTQAGPAG+LSHKVFSSIYNTVK
Sbjct: 181 IIGDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVK 240

Query: 241 HVFRYVIAYTAHVPSYADSCGWVLACDHPLKLDVEDLNNRIRERVQGELRYLDGAFIVSS 300
           HVF+YVIAYTAHVPSYADSCGWVLA DHP++LD EDLNN+I ERV+GELRYLDGAFIVSS
Sbjct: 241 HVFKYVIAYTAHVPSYADSCGWVLASDHPIRLDKEDLNNKIHERVRGELRYLDGAFIVSS 300

Query: 301 TVINKTIRTTVKNETHVFTEEDARFAHGQGL 331
           TVINKTIRT + NETHVFTEEDARFAHG+GL
Sbjct: 301 TVINKTIRTLMMNETHVFTEEDARFAHGRGL 329

[Close]

BLAST of MC08g2577 vs. ExPASy TrEMBL
Match: A0A6J1G4J8 (thermospermine synthase ACAULIS5-like OS=Cucurbita moschata OX=3662 GN=LOC111450716 PE=3 SV=1)

HSP 1 Score: 582 bits (1500), Expect = 1.62e-208
Identity = 282/331 (85.20%), Postives = 304/331 (91.84%), Query Frame = 0


Query: 1   MFSSLCSALSVLFIPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQ 60
           M S LCSAL  L IP +N+TA+P      +S+W+EEQLEDDLKWSFAVNRILHA TS+FQ
Sbjct: 1   MLSPLCSALPALSIPTSNSTAVP-----HVSEWFEEQLEDDLKWSFAVNRILHAATSKFQ 60

Query: 61  DVVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTAR 120
           D+VLLDTKRFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHH+PKTVFI+GGGEGSTAR
Sbjct: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTAR 120

Query: 121 ETLKHKDIEKVVMCDIDREVVNFCNAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDV 180
           ETLKHK IEKVVMCDIDR+VVNFC AHL+ENQDAFQDEKLHIIFDDAK GLEGQPEKFDV
Sbjct: 121 ETLKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDV 180

Query: 181 IIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHKVFSSIYNTVK 240
           II DLADPHEGGPCNHLYTKSFYE  IKPKLNDNGIFVTQAGP+G+LS+ VFSSIYNTVK
Sbjct: 181 IISDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVK 240

Query: 241 HVFRYVIAYTAHVPSYADSCGWVLACDHPLKLDVEDLNNRIRERVQGELRYLDGAFIVSS 300
           HVF+YVIAYTAH+PSYADS GWVLA DHP KLD+EDL+ RIRERV+GELRYLDGAFIVSS
Sbjct: 241 HVFKYVIAYTAHIPSYADSSGWVLASDHPFKLDIEDLDKRIRERVRGELRYLDGAFIVSS 300

Query: 301 TVINKTIRTTVKNETHVFTEEDARFAHGQGL 331
           TVINKTIRT + NETHVFTEEDARF HGQGL
Sbjct: 301 TVINKTIRTAMMNETHVFTEEDARFTHGQGL 326

[Close]

BLAST of MC08g2577 vs. ExPASy TrEMBL
Match: A0A6J1KJ05 (thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC111493662 PE=3 SV=1)

HSP 1 Score: 577 bits (1488), Expect = 1.09e-206
Identity = 280/331 (84.59%), Postives = 304/331 (91.84%), Query Frame = 0


Query: 1   MFSSLCSALSVLFIPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQ 60
           M S LCSAL  L IP +N+TA+P     Q+S+W+EEQLEDDLKWSFAV RILHA TS+FQ
Sbjct: 1   MLSPLCSALPALSIPTSNSTAVP-----QVSEWFEEQLEDDLKWSFAVTRILHAATSKFQ 60

Query: 61  DVVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTAR 120
           D+VLLDTKRFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHH+PKTVFI+GGGEGSTAR
Sbjct: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTAR 120

Query: 121 ETLKHKDIEKVVMCDIDREVVNFCNAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDV 180
           ETLKHK IEKVVMCDIDR+VVNFC AHL+ENQDAFQDEKLHIIF+DAK GLEGQPEKFDV
Sbjct: 121 ETLKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFNDAKVGLEGQPEKFDV 180

Query: 181 IIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHKVFSSIYNTVK 240
           II DLADPHEGGPCNHLYTKSFYE  IKPKLNDNGIFVTQAGP+G+LS+ VFSSIYNTVK
Sbjct: 181 IISDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVK 240

Query: 241 HVFRYVIAYTAHVPSYADSCGWVLACDHPLKLDVEDLNNRIRERVQGELRYLDGAFIVSS 300
           HVF+YVIAYTAH+PSYADS GWVLA DHP +LD+EDL+ RIRERV+GELRYLDGAFIVSS
Sbjct: 241 HVFKYVIAYTAHIPSYADSSGWVLASDHPFELDMEDLDKRIRERVRGELRYLDGAFIVSS 300

Query: 301 TVINKTIRTTVKNETHVFTEEDARFAHGQGL 331
           TVINKTIRT + NETHVFTEEDARF HGQGL
Sbjct: 301 TVINKTIRTAMMNETHVFTEEDARFTHGQGL 326

[Close]

BLAST of MC08g2577 vs. TAIR 10
Match: AT5G19530.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 429.5 bits (1103), Expect = 2.5e-120
Identity = 200/319 (62.70%), Postives = 251/319 (78.68%), Query Frame = 0


Query: 14  IPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQDVVLLDTKRFGKA 73
           I  A +    L  ++Q   WYEE ++DDLKWSFA+N +LH  TSE+QD+ LLDTKRFGK 
Sbjct: 16  IHKATSPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKV 75

Query: 74  LLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTARETLKHKDIEKVVM 133
           L++DGK+QSAE+DEFIYHE L+HPALL H +PKTVFIMGGGEGS ARE LKH  IEKVVM
Sbjct: 76  LVIDGKMQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVM 135

Query: 134 CDIDREVVNFCNAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDVIIGDLADPHEGGP 193
           CDID+EVV+FC   LT N DAF ++KL ++  DAKA LE + EKFD+I+GDLADP EGGP
Sbjct: 136 CDIDQEVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGP 195

Query: 194 CNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHK-VFSSIYNTVKHVFRYVIAYTAH 253
           C  LYTKSFY+ ++KPKL+ NGIFVTQAGPAG+ +HK VF+SIYNT+K VF+YV AYTAH
Sbjct: 196 CYQLYTKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAH 255

Query: 254 VPSYADSCGWVLACDHPLKLDVEDLNNRIRERVQGELRYLDGAFIVSSTVINKTIRTTVK 313
           VPS+AD+ GWV+A DH   ++V++++ RI ERV GEL YL+    VS+  +NKTI   ++
Sbjct: 256 VPSFADTWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLNKTISLALE 315

Query: 314 NETHVFTEEDARFAHGQGL 332
            ET V++EE+ARF HG G+
Sbjct: 316 KETEVYSEENARFIHGHGV 334

[Close]

BLAST of MC08g2577 vs. TAIR 10
Match: AT5G19530.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 429.5 bits (1103), Expect = 2.5e-120
Identity = 200/319 (62.70%), Postives = 251/319 (78.68%), Query Frame = 0


Query: 14  IPNANATALPLQHDRQLSQWYEEQLEDDLKWSFAVNRILHATTSEFQDVVLLDTKRFGKA 73
           I  A +    L  ++Q   WYEE ++DDLKWSFA+N +LH  TSE+QD+ LLDTKRFGK 
Sbjct: 9   IHKATSPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKV 68

Query: 74  LLLDGKLQSAEKDEFIYHESLVHPALLLHHSPKTVFIMGGGEGSTARETLKHKDIEKVVM 133
           L++DGK+QSAE+DEFIYHE L+HPALL H +PKTVFIMGGGEGS ARE LKH  IEKVVM
Sbjct: 69  LVIDGKMQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVM 128

Query: 134 CDIDREVVNFCNAHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDVIIGDLADPHEGGP 193
           CDID+EVV+FC   LT N DAF ++KL ++  DAKA LE + EKFD+I+GDLADP EGGP
Sbjct: 129 CDIDQEVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGP 188

Query: 194 CNHLYTKSFYEGVIKPKLNDNGIFVTQAGPAGMLSHK-VFSSIYNTVKHVFRYVIAYTAH 253
           C  LYTKSFY+ ++KPKL+ NGIFVTQAGPAG+ +HK VF+SIYNT+K VF+YV AYTAH
Sbjct: 189 CYQLYTKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAH 248

Query: 254 VPSYADSCGWVLACDHPLKLDVEDLNNRIRERVQGELRYLDGAFIVSSTVINKTIRTTVK 313
           VPS+AD+ GWV+A DH   ++V++++ RI ERV GEL YL+    VS+  +NKTI   ++
Sbjct: 249 VPSFADTWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLNKTISLALE 308

Query: 314 NETHVFTEEDARFAHGQGL 332
            ET V++EE+ARF HG G+
Sbjct: 309 KETEVYSEENARFIHGHGV 327

[Close]

BLAST of MC08g2577 vs. TAIR 10
Match: AT1G23820.2 (spermidine synthase 1 )

HSP 1 Score: 126.7 bits (317), Expect = 3.4e-29
Identity = 70/224 (31.25%), Postives = 118/224 (52.68%), Query Frame = 0


Query: 45  SFAVNRILHATTSEFQDVVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHS 104
           S  V ++L    S++QDV++  +  +GK L+LDG +Q  E+DE  Y E + H  L    +
Sbjct: 105 SLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPN 164

Query: 105 PKTVFIMGGGEGSTARETLKHKDIEKVVMCDIDREVVNFCNAHLTENQDAFQDEKLHIIF 164
           PK V ++GGG+G   RE  +H  IE++ MC+ID+ VV+       +    ++D +++++ 
Sbjct: 165 PKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVI 224

Query: 165 DDAKAGLEGQPE-KFDVIIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGP 224
            D  A L+   E  +D +I D +DP   GP   L+ K F++ V +  L   G+  TQA  
Sbjct: 225 GDGVAFLKNAAEGSYDAVIVDSSDPI--GPAKELFEKPFFQSVAR-ALRPGGVVCTQA-E 284

Query: 225 AGMLSHKVFSSIYNTVKHVFRYVIAYT-AHVPSYADSCG-WVLA 266
           +  L   +   I +  + +F+  + Y    VP+Y    G W+ A
Sbjct: 285 SLWLHMDIIEDIVSNCREIFKGSVNYAWTSVPTYPRHWGHWIYA 324

[Close]

BLAST of MC08g2577 vs. TAIR 10
Match: AT1G70310.1 (spermidine synthase 2 )

HSP 1 Score: 125.2 bits (313), Expect = 1.0e-28
Identity = 69/224 (30.80%), Postives = 116/224 (51.79%), Query Frame = 0


Query: 45  SFAVNRILHATTSEFQDVVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHS 104
           S  V +IL    S++QDV++  +  +GK L+LDG +Q  E+DE  Y E + H  L    +
Sbjct: 65  SLKVEKILFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSISN 124

Query: 105 PKTVFIMGGGEGSTARETLKHKDIEKVVMCDIDREVVNFCNAHLTENQDAFQDEKLHIIF 164
           PK V ++GGG+G   RE  +H  +E++ +C+ID+ VV+    +       ++D ++++I 
Sbjct: 125 PKKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDVAKQYFPNVAVGYEDPRVNLII 184

Query: 165 DDAKAGLEGQPE-KFDVIIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGP 224
            D  A L+   E  +D +I D +DP   GP   L+ K F+E V +  L   G+  TQA  
Sbjct: 185 GDGVAFLKNAAEGTYDAVIVDSSDPI--GPAKELFEKPFFESVNR-ALRPGGVVCTQA-E 244

Query: 225 AGMLSHKVFSSIYNTVKHVFRYVIAYT-AHVPSYADSCGWVLAC 267
           +  L   +   I +  + +F+  + Y    VP+Y       + C
Sbjct: 245 SLWLHMDIIEDIVSNCRDIFKGSVNYAWTSVPTYPSGVIGFMLC 284

[Close]

BLAST of MC08g2577 vs. TAIR 10
Match: AT1G23820.1 (spermidine synthase 1 )

HSP 1 Score: 125.2 bits (313), Expect = 1.0e-28
Identity = 68/224 (30.36%), Postives = 116/224 (51.79%), Query Frame = 0


Query: 45  SFAVNRILHATTSEFQDVVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHS 104
           S  V ++L    S++QDV++  +  +GK L+LDG +Q  E+DE  Y E + H  L    +
Sbjct: 105 SLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPN 164

Query: 105 PKTVFIMGGGEGSTARETLKHKDIEKVVMCDIDREVVNFCNAHLTENQDAFQDEKLHIIF 164
           PK V ++GGG+G   RE  +H  IE++ MC+ID+ VV+       +    ++D +++++ 
Sbjct: 165 PKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVI 224

Query: 165 DDAKAGLEGQPE-KFDVIIGDLADPHEGGPCNHLYTKSFYEGVIKPKLNDNGIFVTQAGP 224
            D  A L+   E  +D +I D +DP   GP   L+ K F++ V +  L   G+  TQA  
Sbjct: 225 GDGVAFLKNAAEGSYDAVIVDSSDPI--GPAKELFEKPFFQSVAR-ALRPGGVVCTQA-E 284

Query: 225 AGMLSHKVFSSIYNTVKHVFRYVIAYT-AHVPSYADSCGWVLAC 267
           +  L   +   I +  + +F+  + Y    VP+Y       + C
Sbjct: 285 SLWLHMDIIEDIVSNCREIFKGSVNYAWTSVPTYPSGVIGFMLC 324

The following BLAST results are available for this feature:

BLAST of MC08g2577 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9S7X6	3.5e-119	62.70	Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV...	[more]
Q975S5	1.9e-56	41.58	Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / ...	[more]
Q72K55	4.6e-55	40.77	Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / ...	[more]
Q5SK28	4.6e-55	40.77	Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC 27634 / DS...	[more]
Q9YE02	2.5e-53	39.80	Polyamine aminopropyltransferase OS=Aeropyrum pernix (strain ATCC 700893 / DSM 1...	[more]

BLAST of MC08g2577 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038890496.1	4.17e-221	89.12	thermospermine synthase ACAULIS5-like [Benincasa hispida]	[more]
XP_004141174.1	3.42e-220	89.12	thermospermine synthase ACAULIS5 [Cucumis sativus] >KGN60515.1 hypothetical prot...	[more]
XP_008459675.1	6.61e-218	88.82	PREDICTED: thermospermine synthase ACAULIS5-like [Cucumis melo] >KAA0061473.1 th...	[more]
XP_022946737.1	3.34e-208	85.20	thermospermine synthase ACAULIS5-like [Cucurbita moschata]	[more]
KAG7030499.1	3.34e-208	85.20	Thermospermine synthase ACAULIS5 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of MC08g2577 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LET5	1.66e-220	89.12	PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000240 PE=3 S...	[more]
A0A5A7UZV1	3.20e-218	88.82	Thermospermine synthase ACAULIS5-like OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A1S3CAR3	3.20e-218	88.82	thermospermine synthase ACAULIS5-like OS=Cucumis melo OX=3656 GN=LOC103498720 PE...	[more]
A0A6J1G4J8	1.62e-208	85.20	thermospermine synthase ACAULIS5-like OS=Cucurbita moschata OX=3662 GN=LOC111450...	[more]
A0A6J1KJ05	1.09e-206	84.59	thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC11149366...	[more]

BLAST of MC08g2577 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G19530.1	2.5e-120	62.70	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT5G19530.2	2.5e-120	62.70	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G23820.2	3.4e-29	31.25	spermidine synthase 1	[more]
AT1G70310.1	1.0e-28	30.80	spermidine synthase 2	[more]
AT1G23820.1	1.0e-28	30.36	spermidine synthase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR035246	Spermidine synthase, tetramerisation domain	PFAM	PF17284	Spermine_synt_N	coord: 32..84 e-value: 1.8E-12 score: 46.9
IPR037163	Spermidine synthase, tetramerisation domain superfamily	GENE3D	2.30.140.10	Spermidine synthase, tetramerisation domain	coord: 30..83 e-value: 1.1E-15 score: 59.5
None	No IPR available	PFAM	PF01564	Spermine_synth	coord: 87..259 e-value: 3.0E-36 score: 124.6
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 84..330 e-value: 5.6E-72 score: 244.3
None	No IPR available	PANTHER	PTHR43317:SF6	THERMOSPERMINE SYNTHASE ACAULIS5-LIKE	coord: 18..330
None	No IPR available	PANTHER	PTHR43317	THERMOSPERMINE SYNTHASE ACAULIS5	coord: 18..330
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 112..220 e-value: 3.81473E-5 score: 40.4911
IPR030374	Polyamine biosynthesis domain	PROSITE	PS51006	PABS_2	coord: 31..267 score: 85.513931
IPR001045	Spermidine/spermine synthases	HAMAP	MF_00198	Spermidine_synth	coord: 32..312 score: 29.62141
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 31..311

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g2577.1	MC08g2577.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0006596	polyamine biosynthetic process
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0010487	thermospermine synthase activity
molecular_function	GO:0003824	catalytic activity