MC08g2565 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g2565
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Mitogen-activated protein kinase
Location	MC08: 34329790 .. 34333341 (-)
RNA-Seq Expression	MC08g2565
Synteny	MC08g2565

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CGATCCCGATAAATCCTTTATGCAAGAAGTAGAACTTCCATTTCACTTCCTTCGAAGACTTCTGTACGACCTGCAACGCCACTGAAATCAAATCCGCCATTGATGAAGAAGGATAGCTCCATGAATCCTAACTTGAAGCTCATTCTACCTCCTCCTCACGAAGCTTCCTTCGCCAAGTTCATGTATTTTGAAATTTCTTCTCTCTCTTCCTCTCTTCCTCTCTCTGTGCGCTGCCAACACATTGGGCTTAATTACGTTTATGGTGTTCGCCATCATTGCAGAACCAAGAGTGGTGCTTTCACGGATGGCGATTTGCTTGTCAATAGAGACGGCGTCCGGATTGTTTCTCACAACGAAGACGAAACTGTACGCAATTTTTAGTTTGTTTCCTTAATTTTCCAACTCTTTCTCCATTGCTAGGGTACTGTTACCAGATGGCTCTCCCATTTCCCCCACCAGCTTTGTCAATTCTGGAATTAAATGTGTTTTTCTTTTATTTTCTCGGAGCTGCATGACACTTTACTTTTGCTCTGTTTGGTACCCGAGAAAATTCAGCTCCATACCTGAAAATCAGATCTAATTGCACTTTTTAATTGTTTCGTGGTTGTTTATTTTGTTGATTGTTCAGCCTCCACCAATAAAGCCCTCAGACAGCCATTTGTCTTTAGCAGATTTAGACTCGATAAAAGTCATCGGAAAAGGAAATGGTGGAATAGTTCAATTAGTCCGACATAAATGGACGAACCAGTTTTTTGCTCTAAAGGTTTCGCTCTCTCTCTCTCTCTTTTCTTTTTTAAGTAACGTGTTGTTGAGTACTTGTTGTATATGTTCTTTAAGTAAAATTTCAAGCAGAATCAAAAGTGTGCTAATAATTTCATTAATATTTGAAATAGTTGATCAGCTAGTTCTGGAAATTCATTTGATGGTGAAGCTGTTGCCGTTCAATTAAAAAAGATGATGTTTCTTTCACCATTGCTTTAGCTAGTGAATACAATACAAGCTTTACGTTTGTTTCTGCCGAACTTGCTTACCCTTGCCTCTATCTAGATTATAGAGTTTTCCTATAATATACCTCTTATACAAAGAACGTCCGTGTTTCCTTGTTTATATCTTGTGTTGGAGTTGAGTTCCTTCTGACGCTTTCTTTCTGATTCAGGTGATTCAAATGAACGCTGAGGAATCTCGTTGCAGGCTGGTTGCGCAGGAACTAAAAATTAATCAGTTGGCACATAACCATTACATTGTGGTCTGTTACCAGATCTTCTATGACAATGGTGCCATTTTCATCATCTTGGAGTACATGGATGGTGGGTCGCTCGCAGATCTTCTAAAACAGGTCAAAACGATTCTGGAGCCGTATCTTGCAGCCATTTGTTACCAAGTAGGGGGAAGTTTAAAGCACGTCCGATTTGTGAAAATCTAAACGAAAAATTTAAAGTTGACGATTTTTTTTTCTTCCAATTAATATAGGTACTCAAGGGGTTAATATACCTTCACCATGAAAAACATGTCATCCACAGAGACCTTAAGCCTTCTAATTTGTTGATAAATCATAGAGGGGAAGTTAAGATTACTGATTTTGGTGTTAGCGCTATATTGGCAAGTACATCTGGTCAGGCAAATTCTTTCGTTGGCACATTTCCTTACATGTCTGTAAGTGACTTAACATGAACCTAATCTCAGTAGCTATTGCTTGGTGTTTGTTCACTGTTTTTGCTTCACTAATGTATGGTATAGCACTCTAATTTCTACTAGTTTCACTGCAACGATTGCAGCCCGAGAGAATCAGTGGGGAAAAATATGATAACAGAAGTGACATATGGAGTTTAGGATTGGTGTTGCTAGAATGTGCAACGGGTCAATTTCCTTACACTCCACCGGACAAGGCTAAAGGATGGGGAAGCTTTTATGATGTTATGCTAGCCGTAGTAGATCATGCATCGCCTTCTGCTCCTGAAGAGTTTTCACCAGAGTTTTGCTCTTTCATTTCTTCATGGTAAACAAGCTTTTGTGTTTACTATTTGTATGGAATTGGTTTCTGCTGTTTTTGAAAGTTGGTTGGAAGACAGGTTTCATATCTCTACCCTAAATTTCTGAAAACACTTTCAAATATGCCCTGTCTGTTTTGGTTTTATATGTTGCTATTTAGGATTCTCTCCTTATCATAAATTGGATTTTTATATTTTGGTCAGCTTACAGAAAGACCCGCAGAAGAGAAGCTCTGCACGCCAACTTCTGGTATAATGATCCTTCTCAAGTTCTTTATTGCCAATGATTGAATTAGAACATGTCAAGTTGGGATCTGAATGCCAATTATATCTTGTTAATTGGATAATTTTTTATTTTGTAAGATGCATCCTTTCATCAAAAAGTTCGAACGCTTTGATGTCGATCTCGCAGCTTATTTCAAAGATGCTGGCTCTCCATTGGCAACATTCTAATGTTATACGGTACCTTGTGATCTCTCATTGATCTTGAGCTTGAAATTCAGCATTTTTTTATTTGAAGCGTGATACACAAAATATTTACTTACTCACTCGTTTTACCCGAGATTCCGTGTATGAATAGTGAGATTTACTTGGTATCTATACTTCATTCAAGTTTCCTTTAAATCTCACTTTTACCATCTAACTTTGGAAAACTTGGTCGCCAGGAGCAGAAGTCAGGCACAGCCTCTTCCACTTTGACATCTTATTCATTCACCCAATGTTTTTTGATATGGCATCAACTTTTGGAAGTGTTTGTGGAGGAAACAGCTGCTAAAAGTTCAAACTTCATAGATGAAATTCAAGAAGCCAATTCATTTGATCATGAGTAACTCACTCCTGATGTAAACTATGTGAAGCTCGTTTTAAAATAGTGTATGTATGTGTGTTTTTTTAAAAGAAAAATATGAGGAATGTGATTAATTGTTTCCACTGATGTAATGGAAATCGTTCTAGGCAGCCATGGGAAAACTTTTGGGTACAATATTTAGTGTTACTTTAATGGAAAATTGCTTCTACTTGCTTGATTTTTTTTTTCTGTTATAGTGTTATTTATAACAACATAAATACATATTGCCTGTCAACGAGTCGACTTAACTTACCTACACTTTGACTAATTTCAGGGAATTAAACACGGAAATTCGCTTGATTCATGATCGGTTAATTGACCTTCAAAAAAGGGAAAAAAGTAAGAAAGAAAAGAGCCTCCAATGAAGTGTATAAACTTTAAGAACATTTGAAGTAGTTTGTAGCACAAAACAATACTATACATTGGGAGATTTTACTATAACTATACAACAAATAATAATCAAGTTGTTCTTTCCTTTGGGGGGAAAAAAGCCATAAGAATGCAAAGCCAAACATTCCAATTCAGGAAGATGGAAATGTCACTCTTGACCTCCATTCCTGTATGGCAGAACGCAGAGTATGGTTTGGAATAAACATAGAATGCTGGAGCTTAAGCTTTGTCACGGGCGATAAGTCATGTTTCTCGAGCCACGCTTTAATCGCTACGTACTCGTAAGTAAACCCATCTGCTGCTATATAAGGATCATTCATTATTTCC

mRNA sequence

CGATCCCGATAAATCCTTTATGCAAGAAGTAGAACTTCCATTTCACTTCCTTCGAAGACTTCTGTACGACCTGCAACGCCACTGAAATCAAATCCGCCATTGATGAAGAAGGATAGCTCCATGAATCCTAACTTGAAGCTCATTCTACCTCCTCCTCACGAAGCTTCCTTCGCCAAGTTCATAACCAAGAGTGGTGCTTTCACGGATGGCGATTTGCTTGTCAATAGAGACGGCGTCCGGATTGTTTCTCACAACGAAGACGAAACTCCTCCACCAATAAAGCCCTCAGACAGCCATTTGTCTTTAGCAGATTTAGACTCGATAAAAGTCATCGGAAAAGGAAATGGTGGAATAGTTCAATTAGTCCGACATAAATGGACGAACCAGTTTTTTGCTCTAAAGGTGATTCAAATGAACGCTGAGGAATCTCGTTGCAGGCTGGTTGCGCAGGAACTAAAAATTAATCAGTTGGCACATAACCATTACATTGTGGTCTGTTACCAGATCTTCTATGACAATGGTGCCATTTTCATCATCTTGGAGTACATGGATGGTGGGTCGCTCGCAGATCTTCTAAAACAGGTCAAAACGATTCTGGAGCCGTATCTTGCAGCCATTTGTTACCAAGTACTCAAGGGGTTAATATACCTTCACCATGAAAAACATGTCATCCACAGAGACCTTAAGCCTTCTAATTTGTTGATAAATCATAGAGGGGAAGTTAAGATTACTGATTTTGGTGTTAGCGCTATATTGGCAAGTACATCTGGTCAGGCAAATTCTTTCGTTGGCACATTTCCTTACATGTCTCCCGAGAGAATCAGTGGGGAAAAATATGATAACAGAAGTGACATATGGAGTTTAGGATTGGTGTTGCTAGAATGTGCAACGGGTCAATTTCCTTACACTCCACCGGACAAGGCTAAAGGATGGGGAAGCTTTTATGATGTTATGCTAGCCGTAGTAGATCATGCATCGCCTTCTGCTCCTGAAGAGTTTTCACCAGAGTTTTGCTCTTTCATTTCTTCATGCTTACAGAAAGACCCGCAGAAGAGAAGCTCTGCACGCCAACTTCTGATGCATCCTTTCATCAAAAAGTTCGAACGCTTTGATGTCGATCTCGCAGCTTATTTCAAAGATGCTGGCTCTCCATTGGCAACATTCTAATGTTATACGGAGCAGAAGTCAGGCACAGCCTCTTCCACTTTGACATCTTATTCATTCACCCAATGTTTTTTGATATGGCATCAACTTTTGGAAGTGTTTGTGGAGGAAACAGCTGCTAAAAGTTCAAACTTCATAGATGAAATTCAAGAAGCCAATTCATTTGATCATGAGTAACTCACTCCTGATGTAAACTATGTGAAGCTCGTTTTAAAATAGTGTATGTATGTGTGTTTTTTTAAAAGAAAAATATGAGGAATGTGATTAATTGTTTCCACTGATGTAATGGAAATCGTTCTAGGCAGCCATGGGAAAACTTTTGGGTACAATATTTAGTGTTACTTTAATGGAAAATTGCTTCTACTTGCTTGATTTTTTTTTTCTGTTATAGTGTTATTTATAACAACATAAATACATATTGCCTGTCAACGAGTCGACTTAACTTACCTACACTTTGACTAATTTCAGGGAATTAAACACGGAAATTCGCTTGATTCATGATCGGTTAATTGACCTTCAAAAAAGGGAAAAAAGTAAGAAAGAAAAGAGCCTCCAATGAAGTGTATAAACTTTAAGAACATTTGAAGTAGTTTGTAGCACAAAACAATACTATACATTGGGAGATTTTACTATAACTATACAACAAATAATAATCAAGTTGTTCTTTCCTTTGGGGGGAAAAAAGCCATAAGAATGCAAAGCCAAACATTCCAATTCAGGAAGATGGAAATGTCACTCTTGACCTCCATTCCTGTATGGCAGAACGCAGAGTATGGTTTGGAATAAACATAGAATGCTGGAGCTTAAGCTTTGTCACGGGCGATAAGTCATGTTTCTCGAGCCACGCTTTAATCGCTACGTACTCGTAAGTAAACCCATCTGCTGCTATATAAGGATCATTCATTATTTCC

Coding sequence (CDS)

ATGAAGAAGGATAGCTCCATGAATCCTAACTTGAAGCTCATTCTACCTCCTCCTCACGAAGCTTCCTTCGCCAAGTTCATAACCAAGAGTGGTGCTTTCACGGATGGCGATTTGCTTGTCAATAGAGACGGCGTCCGGATTGTTTCTCACAACGAAGACGAAACTCCTCCACCAATAAAGCCCTCAGACAGCCATTTGTCTTTAGCAGATTTAGACTCGATAAAAGTCATCGGAAAAGGAAATGGTGGAATAGTTCAATTAGTCCGACATAAATGGACGAACCAGTTTTTTGCTCTAAAGGTGATTCAAATGAACGCTGAGGAATCTCGTTGCAGGCTGGTTGCGCAGGAACTAAAAATTAATCAGTTGGCACATAACCATTACATTGTGGTCTGTTACCAGATCTTCTATGACAATGGTGCCATTTTCATCATCTTGGAGTACATGGATGGTGGGTCGCTCGCAGATCTTCTAAAACAGGTCAAAACGATTCTGGAGCCGTATCTTGCAGCCATTTGTTACCAAGTACTCAAGGGGTTAATATACCTTCACCATGAAAAACATGTCATCCACAGAGACCTTAAGCCTTCTAATTTGTTGATAAATCATAGAGGGGAAGTTAAGATTACTGATTTTGGTGTTAGCGCTATATTGGCAAGTACATCTGGTCAGGCAAATTCTTTCGTTGGCACATTTCCTTACATGTCTCCCGAGAGAATCAGTGGGGAAAAATATGATAACAGAAGTGACATATGGAGTTTAGGATTGGTGTTGCTAGAATGTGCAACGGGTCAATTTCCTTACACTCCACCGGACAAGGCTAAAGGATGGGGAAGCTTTTATGATGTTATGCTAGCCGTAGTAGATCATGCATCGCCTTCTGCTCCTGAAGAGTTTTCACCAGAGTTTTGCTCTTTCATTTCTTCATGCTTACAGAAAGACCCGCAGAAGAGAAGCTCTGCACGCCAACTTCTGATGCATCCTTTCATCAAAAAGTTCGAACGCTTTGATGTCGATCTCGCAGCTTATTTCAAAGATGCTGGCTCTCCATTGGCAACATTCTAA

Protein sequence

MKKDSSMNPNLKLILPPPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPPIKPSDSHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKINQLAHNHYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKQVKTILEPYLAAICYQVLKGLIYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPERISGEKYDNRSDIWSLGLVLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAPEEFSPEFCSFISSCLQKDPQKRSSARQLLMHPFIKKFERFDVDLAAYFKDAGSPLATF

Homology

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BLAST of MC08g2565 vs. ExPASy Swiss-Prot
Match: Q9S7U9 (Mitogen-activated protein kinase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=MKK2 PE=1 SV=2)

HSP 1 Score: 462.6 bits (1189), Expect = 4.0e-129
Identity = 226/354 (63.84%), Postives = 278/354 (78.53%), Query Frame = 0


Query: 1   MKKDSSMNPNLKLILPPPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPPIK 60
           MKK    N NLKL +P   E S  KF+T+SG F DGDL VN+DGVRI+S  E E   PIK
Sbjct: 1   MKKGGFSN-NLKLAIPVAGEQSITKFLTQSGTFKDGDLRVNKDGVRIISQLEPEVLSPIK 60

Query: 61  PSDSHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI 120
           P+D  LSL+DLD +KVIGKG+ G+VQLV+HKWT QFFALKVIQ+N +E+  + +AQELKI
Sbjct: 61  PADDQLSLSDLDMVKVIGKGSSGVVQLVQHKWTGQFFALKVIQLNIDEAIRKAIAQELKI 120

Query: 121 NQLAHNHYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKQVKTILEPYLAAICYQVLKGL 180
           NQ +    +V  YQ FYDNGAI +ILEYMDGGSLAD LK VK I + YL+AI  QVL+GL
Sbjct: 121 NQSSQCPNLVTSYQSFYDNGAISLILEYMDGGSLADFLKSVKAIPDSYLSAIFRQVLQGL 180

Query: 181 IYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPERI 240
           IYLHH++H+IHRDLKPSNLLINHRGEVKITDFGVS ++ +T+G AN+FVGT+ YMSPERI
Sbjct: 181 IYLHHDRHIIHRDLKPSNLLINHRGEVKITDFGVSTVMTNTAGLANTFVGTYNYMSPERI 240

Query: 241 SGEKYDNRSDIWSLGLVLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAPE-EF 300
            G KY N+SDIWSLGLV+LECATG+FPY PP++ + W S +++M A+VD   P+ P   F
Sbjct: 241 VGNKYGNKSDIWSLGLVVLECATGKFPYAPPNQEETWTSVFELMEAIVDQPPPALPSGNF 300

Query: 301 SPEFCSFISSCLQKDPQKRSSARQLLMHPFIKKFERFDVDLAAYFKDAGSPLAT 354
           SPE  SFIS+CLQKDP  RSSA++L+ HPF+ K++   ++LA+YF DAGSPLAT
Sbjct: 301 SPELSSFISTCLQKDPNSRSSAKELMEHPFLNKYDYSGINLASYFTDAGSPLAT 353

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BLAST of MC08g2565 vs. ExPASy Swiss-Prot
Match: Q94A06 (Mitogen-activated protein kinase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=MKK1 PE=1 SV=2)

HSP 1 Score: 458.4 bits (1178), Expect = 7.5e-128
Identity = 225/340 (66.18%), Postives = 270/340 (79.41%), Query Frame = 0


Query: 17  PPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPPIKPSDSHLSLADLDSIKV 76
           PP E S +KF+T+SG F DGDL VN+DG++ VS +E   PPPI+P D+ LSLADL+ IKV
Sbjct: 14  PPLEQSISKFLTQSGTFKDGDLRVNKDGIQTVSLSEPGAPPPIEPLDNQLSLADLEVIKV 73

Query: 77  IGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKINQLAHNHYIVVCYQIF 136
           IGKG+ G VQLV+HK T QFFALKVIQ+N EES CR ++QEL+IN  +   Y+V CYQ F
Sbjct: 74  IGKGSSGNVQLVKHKLTQQFFALKVIQLNTEESTCRAISQELRINLSSQCPYLVSCYQSF 133

Query: 137 YDNGAIFIILEYMDGGSLADLLKQVKTILEPYLAAICYQVLKGLIYLHHEKHVIHRDLKP 196
           Y NG + IILE+MDGGSLADLLK+V  + E  L+AIC +VL+GL Y+HHE+ +IHRDLKP
Sbjct: 134 YHNGLVSIILEFMDGGSLADLLKKVGKVPENMLSAICKRVLRGLCYIHHERRIIHRDLKP 193

Query: 197 SNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPERISGEKYDNRSDIWSLGL 256
           SNLLINHRGEVKITDFGVS IL STS  ANSFVGT+PYMSPERISG  Y N+SDIWSLGL
Sbjct: 194 SNLLINHRGEVKITDFGVSKILTSTSSLANSFVGTYPYMSPERISGSLYSNKSDIWSLGL 253

Query: 257 VLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAPEE-FSPEFCSFISSCLQKDP 316
           VLLECATG+FPYTPP+  KGW S Y+++ A+V++  P AP   FSPEFCSFIS C+QKDP
Sbjct: 254 VLLECATGKFPYTPPEHKKGWSSVYELVDAIVENPPPCAPSNLFSPEFCSFISQCVQKDP 313

Query: 317 QKRSSARQLLMHPFIKKFERFDVDLAAYFKDAGS---PLA 353
           + R SA++LL H F+K FE  D +L+AYF DAGS   PLA
Sbjct: 314 RDRKSAKELLEHKFVKMFEDSDTNLSAYFTDAGSLIPPLA 353

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BLAST of MC08g2565 vs. ExPASy Swiss-Prot
Match: Q5QN75 (Mitogen-activated protein kinase kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MKK1 PE=1 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 3.0e-116
Identity = 202/338 (59.76%), Postives = 260/338 (76.92%), Query Frame = 0


Query: 17  PPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPP--IKPSDSHLSLADLDSI 76
           P  E    KF+T SG F DG+L +N+ G++++S    + P    +K  D  LS+ DL+ I
Sbjct: 15  PAQETPVDKFLTASGTFKDGELRLNQRGLQLISEETADEPQSTNLKVEDVQLSMDDLEMI 74

Query: 77  KVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKINQLAHNHYIVVCYQ 136
           +VIGKG+GGIVQLVRHKW    +ALK IQMN +E+  + + QELKINQ   N +IV+C+Q
Sbjct: 75  QVIGKGSGGIVQLVRHKWVGTLYALKGIQMNIQEAVRKQIVQELKINQATQNAHIVLCHQ 134

Query: 137 IFYDNGAIFIILEYMDGGSLADLLKQVKTILEPYLAAICYQVLKGLIYLHHEKHVIHRDL 196
            FY NG I+++LEYMD GSLAD++KQVKTILEPYLA +C QVL+GL+YLHHE+HVIHRD+
Sbjct: 135 SFYHNGVIYLVLEYMDRGSLADIIKQVKTILEPYLAVLCKQVLEGLLYLHHERHVIHRDI 194

Query: 197 KPSNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPERISGEKYDNRSDIWSL 256
           KPSNLL+N +GEVKITDFGVSA+LAS+ GQ ++FVGT+ YM+PERISG  YD +SDIWSL
Sbjct: 195 KPSNLLVNRKGEVKITDFGVSAVLASSMGQRDTFVGTYNYMAPERISGSSYDYKSDIWSL 254

Query: 257 GLVLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAP-EEFSPEFCSFISSCLQK 316
           GLV+LECA G+FPY  P + +GW SFY+++ A+VD   PSAP ++FSPEFC+FISSC+QK
Sbjct: 255 GLVILECAIGRFPYI-PSEGEGWLSFYELLEAIVDQPPPSAPADQFSPEFCAFISSCIQK 314

Query: 317 DPQKRSSARQLLMHPFIKKFERFDVDLAAYFKDAGSPL 352
           DP +R SA +LL HPFIKKFE  D+DL    +    P+
Sbjct: 315 DPAERMSASELLNHPFIKKFEDKDLDLRILVESLEPPM 351

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BLAST of MC08g2565 vs. ExPASy Swiss-Prot
Match: Q9FJV0 (Mitogen-activated protein kinase kinase 6 OS=Arabidopsis thaliana OX=3702 GN=MKK6 PE=1 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 1.9e-110
Identity = 195/342 (57.02%), Postives = 256/342 (74.85%), Query Frame = 0


Query: 1   MKKDSSMNPNLKLILPPPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPPIK 60
           M K  S    LKL + P  E+  + F+T SG F DGD L+N+ G+R+ S  +       K
Sbjct: 1   MVKIKSNLKQLKLSV-PAQESPISSFLTASGTFHDGDFLLNQKGLRLTSDEKQSRQSDSK 60

Query: 61  PSDSHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI 120
             D  ++  DL+++KVIGKG+GG+VQLVRHKW  +FFA+KVIQMN +E   + + QELKI
Sbjct: 61  ELDFEITAEDLETVKVIGKGSGGVVQLVRHKWVGKFFAMKVIQMNIQEEIRKQIVQELKI 120

Query: 121 NQLAHN-HYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKQVKTILEPYLAAICYQVLKG 180
           NQ +    ++VVCY  FY NGA  ++LEYMD GSLAD+++QVKTILEPYLA +C QVL G
Sbjct: 121 NQASSQCPHVVVCYHSFYHNGAFSLVLEYMDRGSLADVIRQVKTILEPYLAVVCKQVLLG 180

Query: 181 LIYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPER 240
           L+YLH+E+HVIHRD+KPSNLL+NH+GEVKI+DFGVSA LAS+ GQ ++FVGT+ YMSPER
Sbjct: 181 LVYLHNERHVIHRDIKPSNLLVNHKGEVKISDFGVSASLASSMGQRDTFVGTYNYMSPER 240

Query: 241 ISGEKYDNRSDIWSLGLVLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAP-EE 300
           ISG  YD  SDIWSLG+ +LECA G+FPY   +  +   SFY+++ A+V++  P+AP ++
Sbjct: 241 ISGSTYDYSSDIWSLGMSVLECAIGRFPYLESEDQQNPPSFYELLAAIVENPPPTAPSDQ 300

Query: 301 FSPEFCSFISSCLQKDPQKRSSARQLLMHPFIKKFERFDVDL 341
           FSPEFCSF+S+C+QKDP  R+S+  LL HPFIKKFE  D+DL
Sbjct: 301 FSPEFCSFVSACIQKDPPARASSLDLLSHPFIKKFEDKDIDL 341

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BLAST of MC08g2565 vs. ExPASy Swiss-Prot
Match: A9RWC9 (Mitogen-activated protein kinase kinase 1a OS=Physcomitrium patens OX=3218 GN=MKK1a PE=2 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 7.3e-107
Identity = 181/335 (54.03%), Postives = 251/335 (74.93%), Query Frame = 0


Query: 10  NLKLILPPPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPPIKPSDSHLSLA 69
           N  L +  P E + + ++T SG F DGD+++N++G+R+VS  +  TP     ++ ++SL+
Sbjct: 12  NSALRVNVPKENAISDYVTASGTFQDGDIMLNKEGIRVVSQTQTSTP----AANGNISLS 71

Query: 70  DLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKINQLAHNHYI 129
           DL+++KV+GKG+GG+VQLVRHKWTN+ +ALKVI MN EE+  + + QELKIN  +   Y+
Sbjct: 72  DLETVKVLGKGSGGVVQLVRHKWTNETYALKVIHMNIEETTRKQIVQELKINHASQCPYV 131

Query: 130 VVCYQIFYDNGAIFIILEYMDGGSLADLLKQV--KTILEPYLAAICYQVLKGLIYLHHEK 189
           V+CY  FY+NG I I+LEYMDGGSLAD++K++  K I EP L  +C QVL GL+YLH  +
Sbjct: 132 VICYHAFYNNGLISIVLEYMDGGSLADIMKELEPKCIKEPNLGVVCKQVLLGLMYLHQTR 191

Query: 190 HVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPERISGEKYDN 249
           H+IHRD+KPSNLL+NH+GEVKI+DFGVSA+LA++    ++FVGT  YMSPER+ G  Y  
Sbjct: 192 HIIHRDIKPSNLLVNHKGEVKISDFGVSAVLANSMAVRDTFVGTCTYMSPERVLGGTYGF 251

Query: 250 RSDIWSLGLVLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAP-EEFSPEFCSF 309
            SDIWSLGL LLECA G++PY PP   +GW +FY+++  +VD   P AP ++FSPEFCSF
Sbjct: 252 DSDIWSLGLTLLECALGKYPYQPPGSEEGWMNFYELLQTIVDQPPPVAPADQFSPEFCSF 311

Query: 310 ISSCLQKDPQKRSSARQLLMHPFIKKFERFDVDLA 342
           IS+CLQKDP+ R +A +LL HPF+ K++  +  LA
Sbjct: 312 ISACLQKDPKCRPTAAELLNHPFVNKYDEEEYGLA 342

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BLAST of MC08g2565 vs. NCBI nr
Match: XP_022154773.1 (mitogen-activated protein kinase kinase 2-like isoform X1 [Momordica charantia] >XP_022154774.1 mitogen-activated protein kinase kinase 2-like isoform X1 [Momordica charantia])

HSP 1 Score: 718 bits (1854), Expect = 2.30e-261
Identity = 354/354 (100.00%), Postives = 354/354 (100.00%), Query Frame = 0


Query: 1   MKKDSSMNPNLKLILPPPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPPIK 60
           MKKDSSMNPNLKLILPPPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPPIK
Sbjct: 1   MKKDSSMNPNLKLILPPPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPPIK 60

Query: 61  PSDSHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI 120
           PSDSHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI
Sbjct: 61  PSDSHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI 120

Query: 121 NQLAHNHYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKQVKTILEPYLAAICYQVLKGL 180
           NQLAHNHYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKQVKTILEPYLAAICYQVLKGL
Sbjct: 121 NQLAHNHYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKQVKTILEPYLAAICYQVLKGL 180

Query: 181 IYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPERI 240
           IYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPERI
Sbjct: 181 IYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPERI 240

Query: 241 SGEKYDNRSDIWSLGLVLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAPEEFS 300
           SGEKYDNRSDIWSLGLVLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAPEEFS
Sbjct: 241 SGEKYDNRSDIWSLGLVLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAPEEFS 300

Query: 301 PEFCSFISSCLQKDPQKRSSARQLLMHPFIKKFERFDVDLAAYFKDAGSPLATF 354
           PEFCSFISSCLQKDPQKRSSARQLLMHPFIKKFERFDVDLAAYFKDAGSPLATF
Sbjct: 301 PEFCSFISSCLQKDPQKRSSARQLLMHPFIKKFERFDVDLAAYFKDAGSPLATF 354

[Close]

BLAST of MC08g2565 vs. NCBI nr
Match: XP_022154775.1 (mitogen-activated protein kinase kinase 2-like isoform X2 [Momordica charantia])

HSP 1 Score: 679 bits (1753), Expect = 3.26e-246
Identity = 339/354 (95.76%), Postives = 339/354 (95.76%), Query Frame = 0


Query: 1   MKKDSSMNPNLKLILPPPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPPIK 60
           MKKDSSMNPNLKLILPPPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPPIK
Sbjct: 1   MKKDSSMNPNLKLILPPPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPPIK 60

Query: 61  PSDSHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI 120
           PSDSHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI
Sbjct: 61  PSDSHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI 120

Query: 121 NQLAHNHYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKQVKTILEPYLAAICYQVLKGL 180
           NQLAHNHYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKQ               VLKGL
Sbjct: 121 NQLAHNHYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKQ---------------VLKGL 180

Query: 181 IYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPERI 240
           IYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPERI
Sbjct: 181 IYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPERI 240

Query: 241 SGEKYDNRSDIWSLGLVLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAPEEFS 300
           SGEKYDNRSDIWSLGLVLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAPEEFS
Sbjct: 241 SGEKYDNRSDIWSLGLVLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAPEEFS 300

Query: 301 PEFCSFISSCLQKDPQKRSSARQLLMHPFIKKFERFDVDLAAYFKDAGSPLATF 354
           PEFCSFISSCLQKDPQKRSSARQLLMHPFIKKFERFDVDLAAYFKDAGSPLATF
Sbjct: 301 PEFCSFISSCLQKDPQKRSSARQLLMHPFIKKFERFDVDLAAYFKDAGSPLATF 339

[Close]

BLAST of MC08g2565 vs. NCBI nr
Match: XP_038889637.1 (mitogen-activated protein kinase kinase 2-like [Benincasa hispida])

HSP 1 Score: 640 bits (1650), Expect = 2.75e-230
Identity = 315/354 (88.98%), Postives = 336/354 (94.92%), Query Frame = 0


Query: 1   MKKDSSMNPNLKLILPPPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPPIK 60
           MKKDSSMNPNLKL+LPP  E SFA FIT+SG FTDGDLLVNRDGVRIVS  +DE+PPPIK
Sbjct: 1   MKKDSSMNPNLKLLLPPADEVSFA-FITRSGTFTDGDLLVNRDGVRIVSQEDDESPPPIK 60

Query: 61  PSDSHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI 120
           PSD+HLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEES CRL+A+ELKI
Sbjct: 61  PSDNHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESYCRLLAKELKI 120

Query: 121 NQLAHNHYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKQVKTILEPYLAAICYQVLKGL 180
           NQLA N YIVVCYQIFYDNGAIFIILEYMDGGSLADLLK+V+TILEPYLAAICYQVLKGL
Sbjct: 121 NQLAQNPYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKKVETILEPYLAAICYQVLKGL 180

Query: 181 IYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPERI 240
           IYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILAST+ QANSFVGT+ YMSPER+
Sbjct: 181 IYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTADQANSFVGTYAYMSPERL 240

Query: 241 SGEKYDNRSDIWSLGLVLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAPEEFS 300
           +G+KYDN+SDIWSLGLVLLECATGQFPY PPDK KGW  F+DVM+AVV+ ASPSAPE+FS
Sbjct: 241 NGDKYDNKSDIWSLGLVLLECATGQFPYAPPDKDKGWECFFDVMVAVVELASPSAPEQFS 300

Query: 301 PEFCSFISSCLQKDPQKRSSARQLLMHPFIKKFERFDVDLAAYFKDAGSPLATF 354
           PEFCSFISSCLQKDPQKRSSAR+LL+HPFIKKFE FDVDLAAYFKDAGSPLATF
Sbjct: 301 PEFCSFISSCLQKDPQKRSSARELLVHPFIKKFEHFDVDLAAYFKDAGSPLATF 353

[Close]

BLAST of MC08g2565 vs. NCBI nr
Match: XP_022937733.1 (mitogen-activated protein kinase kinase 1-like [Cucurbita moschata] >XP_022937734.1 mitogen-activated protein kinase kinase 1-like [Cucurbita moschata] >XP_022937735.1 mitogen-activated protein kinase kinase 1-like [Cucurbita moschata] >XP_023536936.1 mitogen-activated protein kinase kinase 1-like [Cucurbita pepo subsp. pepo] >KAG6586494.1 Mitogen-activated protein kinase kinase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 633 bits (1633), Expect = 1.07e-227
Identity = 315/354 (88.98%), Postives = 331/354 (93.50%), Query Frame = 0


Query: 1   MKKDSSMNPNLKLILPPPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPPIK 60
           MKKDSSMNPNLKL LPPP E SFA FIT+ G F DGDLLVNRDGVRIVS  EDE+PPPIK
Sbjct: 1   MKKDSSMNPNLKLHLPPPEEVSFA-FITRGGTFIDGDLLVNRDGVRIVSQAEDESPPPIK 60

Query: 61  PSDSHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI 120
           PSD+HLSLADLDSIKVIGKGNGG+VQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI
Sbjct: 61  PSDNHLSLADLDSIKVIGKGNGGVVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI 120

Query: 121 NQLAHNHYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKQVKTILEPYLAAICYQVLKGL 180
           NQLA N YIVVCYQIFYDNGAIFIILEYMDGGSLADLLK+V TILEPYLAAICYQVLKGL
Sbjct: 121 NQLAQNPYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKKVGTILEPYLAAICYQVLKGL 180

Query: 181 IYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPERI 240
           IYLHHE+HVIHRDLKPSNLLINHRGEVKITDFGVSAILASTS  A+SFVGT+ +MSPERI
Sbjct: 181 IYLHHEEHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSDLAHSFVGTYTFMSPERI 240

Query: 241 SGEKYDNRSDIWSLGLVLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAPEEFS 300
           SG KYDN+SDIWSLGLVLLECATGQFPYTPPDK KGW  F+DVMLAVV+ ASPSAPE+FS
Sbjct: 241 SGGKYDNKSDIWSLGLVLLECATGQFPYTPPDKDKGWEGFFDVMLAVVELASPSAPEQFS 300

Query: 301 PEFCSFISSCLQKDPQKRSSARQLLMHPFIKKFERFDVDLAAYFKDAGSPLATF 354
           PEFCSFISSCL KDPQ+RSSAR+LL+HPFIKKFE  DVDLAAYFKDAGSPLATF
Sbjct: 301 PEFCSFISSCLHKDPQQRSSARELLVHPFIKKFEHLDVDLAAYFKDAGSPLATF 353

[Close]

BLAST of MC08g2565 vs. NCBI nr
Match: XP_004141060.1 (mitogen-activated protein kinase kinase 2 [Cucumis sativus] >KGN60525.1 hypothetical protein Csa_019218 [Cucumis sativus])

HSP 1 Score: 633 bits (1633), Expect = 1.07e-227
Identity = 309/354 (87.29%), Postives = 334/354 (94.35%), Query Frame = 0


Query: 1   MKKDSSMNPNLKLILPPPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPPIK 60
           MKKD+SMN NLKLILPPP E +F  FIT+SG FTDGDLLVN+DGVRIVS  +DE+PPPIK
Sbjct: 1   MKKDTSMNSNLKLILPPPDELTFG-FITRSGTFTDGDLLVNKDGVRIVSQTDDESPPPIK 60

Query: 61  PSDSHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI 120
           PSD+HLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEES CRLVA+ELKI
Sbjct: 61  PSDNHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESYCRLVAKELKI 120

Query: 121 NQLAHNHYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKQVKTILEPYLAAICYQVLKGL 180
           NQLA N YIVVCYQIFYDNGAIFIILEYMDGGSLADLLK+V+T+LEPYLAAICYQVL GL
Sbjct: 121 NQLAQNPYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKKVETVLEPYLAAICYQVLNGL 180

Query: 181 IYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPERI 240
           IYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILA+T+ QANSFVGT+ YMSPER+
Sbjct: 181 IYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILANTADQANSFVGTYAYMSPERL 240

Query: 241 SGEKYDNRSDIWSLGLVLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAPEEFS 300
           +G+KYDN+SDIWSLGL+LLECATGQFPY PPDK KGW  F+DVM+AVV+ ASPSAPE+FS
Sbjct: 241 NGDKYDNKSDIWSLGLILLECATGQFPYAPPDKEKGWEGFFDVMVAVVELASPSAPEQFS 300

Query: 301 PEFCSFISSCLQKDPQKRSSARQLLMHPFIKKFERFDVDLAAYFKDAGSPLATF 354
           PEFCSFISSCLQKDPQKRSSAR+LL+HPFIKKFE FDVDLAAYFKDAGSPLATF
Sbjct: 301 PEFCSFISSCLQKDPQKRSSARELLVHPFIKKFENFDVDLAAYFKDAGSPLATF 353

[Close]

BLAST of MC08g2565 vs. ExPASy TrEMBL
Match: A0A6J1DL84 (mitogen-activated protein kinase kinase 2-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021944 PE=3 SV=1)

HSP 1 Score: 718 bits (1854), Expect = 1.11e-261
Identity = 354/354 (100.00%), Postives = 354/354 (100.00%), Query Frame = 0


Query: 1   MKKDSSMNPNLKLILPPPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPPIK 60
           MKKDSSMNPNLKLILPPPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPPIK
Sbjct: 1   MKKDSSMNPNLKLILPPPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPPIK 60

Query: 61  PSDSHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI 120
           PSDSHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI
Sbjct: 61  PSDSHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI 120

Query: 121 NQLAHNHYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKQVKTILEPYLAAICYQVLKGL 180
           NQLAHNHYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKQVKTILEPYLAAICYQVLKGL
Sbjct: 121 NQLAHNHYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKQVKTILEPYLAAICYQVLKGL 180

Query: 181 IYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPERI 240
           IYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPERI
Sbjct: 181 IYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPERI 240

Query: 241 SGEKYDNRSDIWSLGLVLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAPEEFS 300
           SGEKYDNRSDIWSLGLVLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAPEEFS
Sbjct: 241 SGEKYDNRSDIWSLGLVLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAPEEFS 300

Query: 301 PEFCSFISSCLQKDPQKRSSARQLLMHPFIKKFERFDVDLAAYFKDAGSPLATF 354
           PEFCSFISSCLQKDPQKRSSARQLLMHPFIKKFERFDVDLAAYFKDAGSPLATF
Sbjct: 301 PEFCSFISSCLQKDPQKRSSARQLLMHPFIKKFERFDVDLAAYFKDAGSPLATF 354

[Close]

BLAST of MC08g2565 vs. ExPASy TrEMBL
Match: A0A6J1DKL0 (mitogen-activated protein kinase kinase 2-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111021944 PE=3 SV=1)

HSP 1 Score: 679 bits (1753), Expect = 1.58e-246
Identity = 339/354 (95.76%), Postives = 339/354 (95.76%), Query Frame = 0


Query: 1   MKKDSSMNPNLKLILPPPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPPIK 60
           MKKDSSMNPNLKLILPPPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPPIK
Sbjct: 1   MKKDSSMNPNLKLILPPPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPPIK 60

Query: 61  PSDSHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI 120
           PSDSHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI
Sbjct: 61  PSDSHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI 120

Query: 121 NQLAHNHYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKQVKTILEPYLAAICYQVLKGL 180
           NQLAHNHYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKQ               VLKGL
Sbjct: 121 NQLAHNHYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKQ---------------VLKGL 180

Query: 181 IYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPERI 240
           IYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPERI
Sbjct: 181 IYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPERI 240

Query: 241 SGEKYDNRSDIWSLGLVLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAPEEFS 300
           SGEKYDNRSDIWSLGLVLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAPEEFS
Sbjct: 241 SGEKYDNRSDIWSLGLVLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAPEEFS 300

Query: 301 PEFCSFISSCLQKDPQKRSSARQLLMHPFIKKFERFDVDLAAYFKDAGSPLATF 354
           PEFCSFISSCLQKDPQKRSSARQLLMHPFIKKFERFDVDLAAYFKDAGSPLATF
Sbjct: 301 PEFCSFISSCLQKDPQKRSSARQLLMHPFIKKFERFDVDLAAYFKDAGSPLATF 339

[Close]

BLAST of MC08g2565 vs. ExPASy TrEMBL
Match: A0A6J1FHN0 (mitogen-activated protein kinase kinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111444044 PE=3 SV=1)

HSP 1 Score: 633 bits (1633), Expect = 5.18e-228
Identity = 315/354 (88.98%), Postives = 331/354 (93.50%), Query Frame = 0


Query: 1   MKKDSSMNPNLKLILPPPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPPIK 60
           MKKDSSMNPNLKL LPPP E SFA FIT+ G F DGDLLVNRDGVRIVS  EDE+PPPIK
Sbjct: 1   MKKDSSMNPNLKLHLPPPEEVSFA-FITRGGTFIDGDLLVNRDGVRIVSQAEDESPPPIK 60

Query: 61  PSDSHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI 120
           PSD+HLSLADLDSIKVIGKGNGG+VQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI
Sbjct: 61  PSDNHLSLADLDSIKVIGKGNGGVVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI 120

Query: 121 NQLAHNHYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKQVKTILEPYLAAICYQVLKGL 180
           NQLA N YIVVCYQIFYDNGAIFIILEYMDGGSLADLLK+V TILEPYLAAICYQVLKGL
Sbjct: 121 NQLAQNPYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKKVGTILEPYLAAICYQVLKGL 180

Query: 181 IYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPERI 240
           IYLHHE+HVIHRDLKPSNLLINHRGEVKITDFGVSAILASTS  A+SFVGT+ +MSPERI
Sbjct: 181 IYLHHEEHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSDLAHSFVGTYTFMSPERI 240

Query: 241 SGEKYDNRSDIWSLGLVLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAPEEFS 300
           SG KYDN+SDIWSLGLVLLECATGQFPYTPPDK KGW  F+DVMLAVV+ ASPSAPE+FS
Sbjct: 241 SGGKYDNKSDIWSLGLVLLECATGQFPYTPPDKDKGWEGFFDVMLAVVELASPSAPEQFS 300

Query: 301 PEFCSFISSCLQKDPQKRSSARQLLMHPFIKKFERFDVDLAAYFKDAGSPLATF 354
           PEFCSFISSCL KDPQ+RSSAR+LL+HPFIKKFE  DVDLAAYFKDAGSPLATF
Sbjct: 301 PEFCSFISSCLHKDPQQRSSARELLVHPFIKKFEHLDVDLAAYFKDAGSPLATF 353

[Close]

BLAST of MC08g2565 vs. ExPASy TrEMBL
Match: A0A0A0LEU1 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000340 PE=3 SV=1)

HSP 1 Score: 633 bits (1633), Expect = 5.18e-228
Identity = 309/354 (87.29%), Postives = 334/354 (94.35%), Query Frame = 0


Query: 1   MKKDSSMNPNLKLILPPPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPPIK 60
           MKKD+SMN NLKLILPPP E +F  FIT+SG FTDGDLLVN+DGVRIVS  +DE+PPPIK
Sbjct: 1   MKKDTSMNSNLKLILPPPDELTFG-FITRSGTFTDGDLLVNKDGVRIVSQTDDESPPPIK 60

Query: 61  PSDSHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI 120
           PSD+HLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEES CRLVA+ELKI
Sbjct: 61  PSDNHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESYCRLVAKELKI 120

Query: 121 NQLAHNHYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKQVKTILEPYLAAICYQVLKGL 180
           NQLA N YIVVCYQIFYDNGAIFIILEYMDGGSLADLLK+V+T+LEPYLAAICYQVL GL
Sbjct: 121 NQLAQNPYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKKVETVLEPYLAAICYQVLNGL 180

Query: 181 IYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPERI 240
           IYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILA+T+ QANSFVGT+ YMSPER+
Sbjct: 181 IYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILANTADQANSFVGTYAYMSPERL 240

Query: 241 SGEKYDNRSDIWSLGLVLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAPEEFS 300
           +G+KYDN+SDIWSLGL+LLECATGQFPY PPDK KGW  F+DVM+AVV+ ASPSAPE+FS
Sbjct: 241 NGDKYDNKSDIWSLGLILLECATGQFPYAPPDKEKGWEGFFDVMVAVVELASPSAPEQFS 300

Query: 301 PEFCSFISSCLQKDPQKRSSARQLLMHPFIKKFERFDVDLAAYFKDAGSPLATF 354
           PEFCSFISSCLQKDPQKRSSAR+LL+HPFIKKFE FDVDLAAYFKDAGSPLATF
Sbjct: 301 PEFCSFISSCLQKDPQKRSSARELLVHPFIKKFENFDVDLAAYFKDAGSPLATF 353

[Close]

BLAST of MC08g2565 vs. ExPASy TrEMBL
Match: A0A6J1HNP5 (mitogen-activated protein kinase kinase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465346 PE=3 SV=1)

HSP 1 Score: 631 bits (1628), Expect = 2.99e-227
Identity = 314/354 (88.70%), Postives = 330/354 (93.22%), Query Frame = 0


Query: 1   MKKDSSMNPNLKLILPPPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPPIK 60
           MKKDSSMNPNLKL LPPP E SFA FIT+ G F DGDLLVNRDGVRIVS  EDE+PPPIK
Sbjct: 1   MKKDSSMNPNLKLHLPPPEEVSFA-FITRGGTFIDGDLLVNRDGVRIVSQAEDESPPPIK 60

Query: 61  PSDSHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI 120
           PSD+HLSLADLDSIKVIGKGNGG+VQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI
Sbjct: 61  PSDNHLSLADLDSIKVIGKGNGGVVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI 120

Query: 121 NQLAHNHYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKQVKTILEPYLAAICYQVLKGL 180
           NQLA N YIVVCYQIFYDNGAIFIILEYMDGGSLADLLK+V TILEPYLAAICYQVLKGL
Sbjct: 121 NQLAQNPYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKKVGTILEPYLAAICYQVLKGL 180

Query: 181 IYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPERI 240
           IYLHHE+HVIHRDLKPSNLLINHRGEVKITDFGVSAILASTS  A+SFVGT+ +MSPERI
Sbjct: 181 IYLHHEEHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSDLAHSFVGTYTFMSPERI 240

Query: 241 SGEKYDNRSDIWSLGLVLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAPEEFS 300
           SG KYDN+SDIWSLGLVLLECATGQFPYTPPDK KGW  F+DVMLAVV+ ASPSAPE+FS
Sbjct: 241 SGGKYDNKSDIWSLGLVLLECATGQFPYTPPDKDKGWEGFFDVMLAVVELASPSAPEQFS 300

Query: 301 PEFCSFISSCLQKDPQKRSSARQLLMHPFIKKFERFDVDLAAYFKDAGSPLATF 354
           PEFCSFISSCL KDPQ+RSSAR+LL+HPFIKKFE  DVDLAAYF DAGSPLATF
Sbjct: 301 PEFCSFISSCLHKDPQQRSSARELLVHPFIKKFEHLDVDLAAYFNDAGSPLATF 353

[Close]

BLAST of MC08g2565 vs. TAIR 10
Match: AT4G29810.1 (MAP kinase kinase 2 )

HSP 1 Score: 462.6 bits (1189), Expect = 2.8e-130
Identity = 226/354 (63.84%), Postives = 278/354 (78.53%), Query Frame = 0


Query: 1   MKKDSSMNPNLKLILPPPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPPIK 60
           MKK    N NLKL +P   E S  KF+T+SG F DGDL VN+DGVRI+S  E E   PIK
Sbjct: 1   MKKGGFSN-NLKLAIPVAGEQSITKFLTQSGTFKDGDLRVNKDGVRIISQLEPEVLSPIK 60

Query: 61  PSDSHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKI 120
           P+D  LSL+DLD +KVIGKG+ G+VQLV+HKWT QFFALKVIQ+N +E+  + +AQELKI
Sbjct: 61  PADDQLSLSDLDMVKVIGKGSSGVVQLVQHKWTGQFFALKVIQLNIDEAIRKAIAQELKI 120

Query: 121 NQLAHNHYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKQVKTILEPYLAAICYQVLKGL 180
           NQ +    +V  YQ FYDNGAI +ILEYMDGGSLAD LK VK I + YL+AI  QVL+GL
Sbjct: 121 NQSSQCPNLVTSYQSFYDNGAISLILEYMDGGSLADFLKSVKAIPDSYLSAIFRQVLQGL 180

Query: 181 IYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPERI 240
           IYLHH++H+IHRDLKPSNLLINHRGEVKITDFGVS ++ +T+G AN+FVGT+ YMSPERI
Sbjct: 181 IYLHHDRHIIHRDLKPSNLLINHRGEVKITDFGVSTVMTNTAGLANTFVGTYNYMSPERI 240

Query: 241 SGEKYDNRSDIWSLGLVLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAPE-EF 300
            G KY N+SDIWSLGLV+LECATG+FPY PP++ + W S +++M A+VD   P+ P   F
Sbjct: 241 VGNKYGNKSDIWSLGLVVLECATGKFPYAPPNQEETWTSVFELMEAIVDQPPPALPSGNF 300

Query: 301 SPEFCSFISSCLQKDPQKRSSARQLLMHPFIKKFERFDVDLAAYFKDAGSPLAT 354
           SPE  SFIS+CLQKDP  RSSA++L+ HPF+ K++   ++LA+YF DAGSPLAT
Sbjct: 301 SPELSSFISTCLQKDPNSRSSAKELMEHPFLNKYDYSGINLASYFTDAGSPLAT 353

[Close]

BLAST of MC08g2565 vs. TAIR 10
Match: AT4G26070.2 (MAP kinase/ ERK kinase 1 )

HSP 1 Score: 458.4 bits (1178), Expect = 5.3e-129
Identity = 225/340 (66.18%), Postives = 270/340 (79.41%), Query Frame = 0


Query: 17  PPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPPIKPSDSHLSLADLDSIKV 76
           PP E S +KF+T+SG F DGDL VN+DG++ VS +E   PPPI+P D+ LSLADL+ IKV
Sbjct: 14  PPLEQSISKFLTQSGTFKDGDLRVNKDGIQTVSLSEPGAPPPIEPLDNQLSLADLEVIKV 73

Query: 77  IGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKINQLAHNHYIVVCYQIF 136
           IGKG+ G VQLV+HK T QFFALKVIQ+N EES CR ++QEL+IN  +   Y+V CYQ F
Sbjct: 74  IGKGSSGNVQLVKHKLTQQFFALKVIQLNTEESTCRAISQELRINLSSQCPYLVSCYQSF 133

Query: 137 YDNGAIFIILEYMDGGSLADLLKQVKTILEPYLAAICYQVLKGLIYLHHEKHVIHRDLKP 196
           Y NG + IILE+MDGGSLADLLK+V  + E  L+AIC +VL+GL Y+HHE+ +IHRDLKP
Sbjct: 134 YHNGLVSIILEFMDGGSLADLLKKVGKVPENMLSAICKRVLRGLCYIHHERRIIHRDLKP 193

Query: 197 SNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPERISGEKYDNRSDIWSLGL 256
           SNLLINHRGEVKITDFGVS IL STS  ANSFVGT+PYMSPERISG  Y N+SDIWSLGL
Sbjct: 194 SNLLINHRGEVKITDFGVSKILTSTSSLANSFVGTYPYMSPERISGSLYSNKSDIWSLGL 253

Query: 257 VLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAPEE-FSPEFCSFISSCLQKDP 316
           VLLECATG+FPYTPP+  KGW S Y+++ A+V++  P AP   FSPEFCSFIS C+QKDP
Sbjct: 254 VLLECATGKFPYTPPEHKKGWSSVYELVDAIVENPPPCAPSNLFSPEFCSFISQCVQKDP 313

Query: 317 QKRSSARQLLMHPFIKKFERFDVDLAAYFKDAGS---PLA 353
           + R SA++LL H F+K FE  D +L+AYF DAGS   PLA
Sbjct: 314 RDRKSAKELLEHKFVKMFEDSDTNLSAYFTDAGSLIPPLA 353

[Close]

BLAST of MC08g2565 vs. TAIR 10
Match: AT4G26070.3 (MAP kinase/ ERK kinase 1 )

HSP 1 Score: 458.4 bits (1178), Expect = 5.3e-129
Identity = 225/340 (66.18%), Postives = 270/340 (79.41%), Query Frame = 0


Query: 17  PPHEASFAKFITKSGAFTDGDLLVNRDGVRIVSHNEDETPPPIKPSDSHLSLADLDSIKV 76
           PP E S +KF+T+SG F DGDL VN+DG++ VS +E   PPPI+P D+ LSLADL+ IKV
Sbjct: 14  PPLEQSISKFLTQSGTFKDGDLRVNKDGIQTVSLSEPGAPPPIEPLDNQLSLADLEVIKV 73

Query: 77  IGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRCRLVAQELKINQLAHNHYIVVCYQIF 136
           IGKG+ G VQLV+HK T QFFALKVIQ+N EES CR ++QEL+IN  +   Y+V CYQ F
Sbjct: 74  IGKGSSGNVQLVKHKLTQQFFALKVIQLNTEESTCRAISQELRINLSSQCPYLVSCYQSF 133

Query: 137 YDNGAIFIILEYMDGGSLADLLKQVKTILEPYLAAICYQVLKGLIYLHHEKHVIHRDLKP 196
           Y NG + IILE+MDGGSLADLLK+V  + E  L+AIC +VL+GL Y+HHE+ +IHRDLKP
Sbjct: 134 YHNGLVSIILEFMDGGSLADLLKKVGKVPENMLSAICKRVLRGLCYIHHERRIIHRDLKP 193

Query: 197 SNLLINHRGEVKITDFGVSAILASTSGQANSFVGTFPYMSPERISGEKYDNRSDIWSLGL 256
           SNLLINHRGEVKITDFGVS IL STS  ANSFVGT+PYMSPERISG  Y N+SDIWSLGL
Sbjct: 194 SNLLINHRGEVKITDFGVSKILTSTSSLANSFVGTYPYMSPERISGSLYSNKSDIWSLGL 253

Query: 257 VLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHASPSAPEE-FSPEFCSFISSCLQKDP 316
           VLLECATG+FPYTPP+  KGW S Y+++ A+V++  P AP   FSPEFCSFIS C+QKDP
Sbjct: 254 VLLECATGKFPYTPPEHKKGWSSVYELVDAIVENPPPCAPSNLFSPEFCSFISQCVQKDP 313

Query: 317 QKRSSARQLLMHPFIKKFERFDVDLAAYFKDAGS---PLA 353
           + R SA++LL H F+K FE  D +L+AYF DAGS   PLA
Sbjct: 314 RDRKSAKELLEHKFVKMFEDSDTNLSAYFTDAGSLIPPLA 353

[Close]

BLAST of MC08g2565 vs. TAIR 10
Match: AT4G29810.2 (MAP kinase kinase 2 )

HSP 1 Score: 454.9 bits (1169), Expect = 5.9e-128
Identity = 226/363 (62.26%), Postives = 278/363 (76.58%), Query Frame = 0


Query: 1   MKKDSSMNPNLKLILPPPHEASFAKFI---------TKSGAFTDGDLLVNRDGVRIVSHN 60
           MKK    N NLKL +P   E S  KF+         T+SG F DGDL VN+DGVRI+S  
Sbjct: 1   MKKGGFSN-NLKLAIPVAGEQSITKFLRKGFGSLCRTQSGTFKDGDLRVNKDGVRIISQL 60

Query: 61  EDETPPPIKPSDSHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRC 120
           E E   PIKP+D  LSL+DLD +KVIGKG+ G+VQLV+HKWT QFFALKVIQ+N +E+  
Sbjct: 61  EPEVLSPIKPADDQLSLSDLDMVKVIGKGSSGVVQLVQHKWTGQFFALKVIQLNIDEAIR 120

Query: 121 RLVAQELKINQLAHNHYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKQVKTILEPYLAA 180
           + +AQELKINQ +    +V  YQ FYDNGAI +ILEYMDGGSLAD LK VK I + YL+A
Sbjct: 121 KAIAQELKINQSSQCPNLVTSYQSFYDNGAISLILEYMDGGSLADFLKSVKAIPDSYLSA 180

Query: 181 ICYQVLKGLIYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSGQANSFVGT 240
           I  QVL+GLIYLHH++H+IHRDLKPSNLLINHRGEVKITDFGVS ++ +T+G AN+FVGT
Sbjct: 181 IFRQVLQGLIYLHHDRHIIHRDLKPSNLLINHRGEVKITDFGVSTVMTNTAGLANTFVGT 240

Query: 241 FPYMSPERISGEKYDNRSDIWSLGLVLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHA 300
           + YMSPERI G KY N+SDIWSLGLV+LECATG+FPY PP++ + W S +++M A+VD  
Sbjct: 241 YNYMSPERIVGNKYGNKSDIWSLGLVVLECATGKFPYAPPNQEETWTSVFELMEAIVDQP 300

Query: 301 SPSAPE-EFSPEFCSFISSCLQKDPQKRSSARQLLMHPFIKKFERFDVDLAAYFKDAGSP 354
            P+ P   FSPE  SFIS+CLQKDP  RSSA++L+ HPF+ K++   ++LA+YF DAGSP
Sbjct: 301 PPALPSGNFSPELSSFISTCLQKDPNSRSSAKELMEHPFLNKYDYSGINLASYFTDAGSP 360

[Close]

BLAST of MC08g2565 vs. TAIR 10
Match: AT4G29810.3 (MAP kinase kinase 2 )

HSP 1 Score: 419.5 bits (1077), Expect = 2.7e-117
Identity = 210/336 (62.50%), Postives = 257/336 (76.49%), Query Frame = 0


Query: 1   MKKDSSMNPNLKLILPPPHEASFAKFI---------TKSGAFTDGDLLVNRDGVRIVSHN 60
           MKK    N NLKL +P   E S  KF+         T+SG F DGDL VN+DGVRI+S  
Sbjct: 1   MKKGGFSN-NLKLAIPVAGEQSITKFLRKGFGSLCRTQSGTFKDGDLRVNKDGVRIISQL 60

Query: 61  EDETPPPIKPSDSHLSLADLDSIKVIGKGNGGIVQLVRHKWTNQFFALKVIQMNAEESRC 120
           E E   PIKP+D  LSL+DLD +KVIGKG+ G+VQLV+HKWT QFFALKVIQ+N +E+  
Sbjct: 61  EPEVLSPIKPADDQLSLSDLDMVKVIGKGSSGVVQLVQHKWTGQFFALKVIQLNIDEAIR 120

Query: 121 RLVAQELKINQLAHNHYIVVCYQIFYDNGAIFIILEYMDGGSLADLLKQVKTILEPYLAA 180
           + +AQELKINQ +    +V  YQ FYDNGAI +ILEYMDGGSLAD LK VK I + YL+A
Sbjct: 121 KAIAQELKINQSSQCPNLVTSYQSFYDNGAISLILEYMDGGSLADFLKSVKAIPDSYLSA 180

Query: 181 ICYQVLKGLIYLHHEKHVIHRDLKPSNLLINHRGEVKITDFGVSAILASTSGQANSFVGT 240
           I  QVL+GLIYLHH++H+IHRDLKPSNLLINHRGEVKITDFGVS ++ +T+G AN+FVGT
Sbjct: 181 IFRQVLQGLIYLHHDRHIIHRDLKPSNLLINHRGEVKITDFGVSTVMTNTAGLANTFVGT 240

Query: 241 FPYMSPERISGEKYDNRSDIWSLGLVLLECATGQFPYTPPDKAKGWGSFYDVMLAVVDHA 300
           + YMSPERI G KY N+SDIWSLGLV+LECATG+FPY PP++ + W S +++M A+VD  
Sbjct: 241 YNYMSPERIVGNKYGNKSDIWSLGLVVLECATGKFPYAPPNQEETWTSVFELMEAIVDQP 300

Query: 301 SPSAPE-EFSPEFCSFISSCLQKDPQKRSSARQLLM 327
            P+ P   FSPE  SFIS+CLQKDP  RSSA++L++
Sbjct: 301 PPALPSGNFSPELSSFISTCLQKDPNSRSSAKELMV 335

The following BLAST results are available for this feature:

BLAST of MC08g2565 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9S7U9	4.0e-129	63.84	Mitogen-activated protein kinase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=MKK...	[more]
Q94A06	7.5e-128	66.18	Mitogen-activated protein kinase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=MKK...	[more]
Q5QN75	3.0e-116	59.76	Mitogen-activated protein kinase kinase 1 OS=Oryza sativa subsp. japonica OX=399...	[more]
Q9FJV0	1.9e-110	57.02	Mitogen-activated protein kinase kinase 6 OS=Arabidopsis thaliana OX=3702 GN=MKK...	[more]
A9RWC9	7.3e-107	54.03	Mitogen-activated protein kinase kinase 1a OS=Physcomitrium patens OX=3218 GN=MK...	[more]

BLAST of MC08g2565 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154773.1	2.30e-261	100.00	mitogen-activated protein kinase kinase 2-like isoform X1 [Momordica charantia] ...	[more]
XP_022154775.1	3.26e-246	95.76	mitogen-activated protein kinase kinase 2-like isoform X2 [Momordica charantia]	[more]
XP_038889637.1	2.75e-230	88.98	mitogen-activated protein kinase kinase 2-like [Benincasa hispida]	[more]
XP_022937733.1	1.07e-227	88.98	mitogen-activated protein kinase kinase 1-like [Cucurbita moschata] >XP_02293773...	[more]
XP_004141060.1	1.07e-227	87.29	mitogen-activated protein kinase kinase 2 [Cucumis sativus] >KGN60525.1 hypothet...	[more]

BLAST of MC08g2565 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DL84	1.11e-261	100.00	mitogen-activated protein kinase kinase 2-like isoform X1 OS=Momordica charantia...	[more]
A0A6J1DKL0	1.58e-246	95.76	mitogen-activated protein kinase kinase 2-like isoform X2 OS=Momordica charantia...	[more]
A0A6J1FHN0	5.18e-228	88.98	mitogen-activated protein kinase kinase 1-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A0A0LEU1	5.18e-228	87.29	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000...	[more]
A0A6J1HNP5	2.99e-227	88.70	mitogen-activated protein kinase kinase 1-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]

BLAST of MC08g2565 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G29810.1	2.8e-130	63.84	MAP kinase kinase 2	[more]
AT4G26070.2	5.3e-129	66.18	MAP kinase/ ERK kinase 1	[more]
AT4G26070.3	5.3e-129	66.18	MAP kinase/ ERK kinase 1	[more]
AT4G29810.2	5.9e-128	62.26	MAP kinase kinase 2	[more]
AT4G29810.3	2.7e-117	62.50	MAP kinase kinase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 71..330 e-value: 2.7E-83 score: 292.7
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 73..330 e-value: 6.8E-69 score: 232.2
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 71..330 score: 46.548222
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 151..349 e-value: 8.3E-61 score: 207.6
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 50..150 e-value: 1.0E-23 score: 85.4
None	No IPR available	PIRSR	PIRSR037568-2	PIRSR037568-2	coord: 66..268 e-value: 3.9E-30 score: 101.9
None	No IPR available	PIRSR	PIRSR037993-2	PIRSR037993-2	coord: 143..335 e-value: 5.6E-19 score: 66.0
None	No IPR available	PANTHER	PTHR24361:SF759	MAP KINASE KINASE	coord: 5..353
None	No IPR available	PANTHER	PTHR24361	MITOGEN-ACTIVATED KINASE KINASE KINASE	coord: 5..353
None	No IPR available	CDD	cd06623	PKc_MAPKK_plant_like	coord: 69..334 e-value: 1.06136E-145 score: 410.445
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 77..100
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 189..201
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 57..335

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g2565.1	MC08g2565.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004674	protein serine/threonine kinase activity
molecular_function	GO:0004672	protein kinase activity