MC08g2452 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g2452 vs. ExPASy Swiss-Prot
Match: Q9SZY4 (Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana OX=3702 GN=NPF6.2 PE=1 SV=1)

HSP 1 Score: 826.6 bits (2134), Expect = 1.7e-238
Identity = 426/583 (73.07%), Postives = 486/583 (83.36%), Query Frame = 0

Query: 1   MDEKQSLTVTDAVDYKGCPASRSKTGGWVPAALILGIELCERLSTMGIAVNLVTYLVGTL 60
           M+ K S TV DAVDYKG PA +SKTGGW+ AALILGIE+ ERLSTMGIAVNLVTYL+ T+
Sbjct: 1   MESKGSWTVADAVDYKGRPADKSKTGGWITAALILGIEVVERLSTMGIAVNLVTYLMETM 60

Query: 61  HLPSAASANIVTDFMGTSFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLQ 120
           HLPS+ SANIVTDFMGTSFLLCLLGGFLADSFLGR+KTI IF++IQ LGTG LAV TKL 
Sbjct: 61  HLPSSTSANIVTDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTIQALGTGALAVATKLP 120

Query: 121 QLHPPPCDPNASKKCKQANGFQMGMLYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK 180
           +L PP C  +  + C  A  FQM +LY+ LYLIALGTGG+KSS+SGFG+DQFD+KD KEK
Sbjct: 121 ELRPPTC--HHGEACIPATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEK 180

Query: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWGYGICSVSMFTAILSFLRGTKRYR 240
           A MA+FFNRFF F+S GTLLAVTVLVY+QDEVGRSW YGIC+VSM  AI+ FL GTKRYR
Sbjct: 181 AHMAFFFNRFFFFISMGTLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYR 240

Query: 241 YKKSMGSPIVHISQVIVAAINKRKMALPFNDALLYEDSAATSRIDHTNQFRFLDKAAIFA 300
           YKKS GSP+V I QVI AA  KRKM LP +   LYED+    RI+HT+QF  LDKAAI A
Sbjct: 241 YKKSQGSPVVQIFQVIAAAFRKRKMELPQSIVYLYEDNPEGIRIEHTDQFHLLDKAAIVA 300

Query: 301 EGDFEKSVN--SAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTAYAQMITFSVEQAS 360
           EGDFE++++  + PNPWKL SVT+VEEVKMM+RLLPIWATTIIFWT YAQMITFSVEQAS
Sbjct: 301 EGDFEQTLDGVAIPNPWKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQAS 360

Query: 361 TMERSFGAFQIPAGSLTVFFVTAILITLALYDRLIMPLWRKWKGKPGFTNLQRIAIGLIL 420
           TM R+ G+F+IPAGSLTVFFV AILITLA+YDR IMP W+KWKGKPGF++LQRIAIGL+L
Sbjct: 361 TMRRNIGSFKIPAGSLTVFFVAAILITLAVYDRAIMPFWKKWKGKPGFSSLQRIAIGLVL 420

Query: 421 STLGMAAAALAETKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFIT 480
           ST GMAAAAL E KRLSVAK+   S  TLP+SVFLL+PQFFLVG+GEAFIYTGQLDFFIT
Sbjct: 421 STAGMAAAALVEQKRLSVAKS--SSQKTLPISVFLLVPQFFLVGAGEAFIYTGQLDFFIT 480

Query: 481 QSPKGMKTVSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGL 540
           QSPKGMKT+STGLFLTTLSLGFFVSSFLV++VKRVT +    GWLADNIN+ RLD FY L
Sbjct: 481 QSPKGMKTMSTGLFLTTLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHGRLDYFYWL 540

Query: 541 LTILSAINFVVFLVCAIWYKPQKPKQLLEMPTSTNEGSVAEKC 582
           L ILS INFVV+++CA+W+KP K K  +E      +G   E C
Sbjct: 541 LVILSGINFVVYIICALWFKPTKGKDSVE--KENGKGFSVEDC 577

BLAST of MC08g2452 vs. ExPASy Swiss-Prot
Match: Q05085 (Protein NRT1/ PTR FAMILY 6.3 OS=Arabidopsis thaliana OX=3702 GN=NPF6.3 PE=1 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 8.7e-166
Identity = 310/578 (53.63%), Postives = 407/578 (70.42%), Query Frame = 0

Query: 2   DEKQSLTVTDAVDYKGCPASRSKTGGWVPAALILGIELCERLSTMGIAVNLVTYLVGTLH 61
           + K    + DA D++G PA RSKTGGW  AA+IL IE  ERL+T+GI VNLVTYL GT+H
Sbjct: 5   ETKSDDILLDAWDFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGTMH 64

Query: 62  LPSAASANIVTDFMGTSFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLQQ 121
           L +A +AN VT+F+GTSF+LCLLGGF+AD+FLGRY TIAIFA+IQ  G   L + T +  
Sbjct: 65  LGNATAANTVTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTIIPG 124

Query: 122 LHPPPCDPNASKKCKQANGFQMGMLYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKA 181
           L PP C+P  S  C+QA+G Q+ +LYL LYL ALGTGG+K+SVSGFG+DQFDE + KE++
Sbjct: 125 LRPPRCNPTTSSHCEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERS 184

Query: 182 QMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWGYGICSVSMFTAILSFLRGTKRYRY 241
           +M YFFNRFF  ++ G+LLAVTVLVY+QD+VGR WGYGIC+ ++  A+  FL GT RYR+
Sbjct: 185 KMTYFFNRFFFCINVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRF 244

Query: 242 KKSMGSPIVHISQVIVAAINKRKMALPFNDALLYE---------DSAATSRIDHTNQFRF 301
           KK +GSP+  ++ VIVAA   RK+ LP + + LY+               ++ HT QFR 
Sbjct: 245 KKLIGSPMTQVAAVIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRS 304

Query: 302 LDKAAIFAEGDFEKSVNS-APNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTAYAQMIT 361
           LDKAAI    D E  V S   N W L ++T VEEVK ++R+LPIWAT I+FWT +AQ+ T
Sbjct: 305 LDKAAI---RDQEAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTT 364

Query: 362 FSVEQASTMERSFGAFQIPAGSLTVFFVTAILITLALYDRLIMPLWRKWKGKP-GFTNLQ 421
            SV Q+ T++RS G+F+IP  S+ VF+V  +L+T A+YDR+ + L +K    P G   LQ
Sbjct: 365 LSVAQSETLDRSIGSFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQ 424

Query: 422 RIAIGLILSTLGMAAAALAETKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYT 481
           RI +GL   ++ MA AAL E KRL  A A G +  TLPL  +LLIPQ+ +VG GEA IYT
Sbjct: 425 RIGLGLFFGSMAMAVAALVELKRLRTAHAHGPTVKTLPLGFYLLIPQYLIVGIGEALIYT 484

Query: 482 GQLDFFITQSPKGMKTVSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYA 541
           GQLDFF+ + PKGMK +STGL L+TL+LGFF SS LV +V++ TG      W+AD++N  
Sbjct: 485 GQLDFFLRECPKGMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGK--AHPWIADDLNKG 544

Query: 542 RLDCFYGLLTILSAINFVVFLVCAIWYKPQKPKQLLEM 569
           RL  FY L+ +L A+NF++FLV + WY   K K+L E+
Sbjct: 545 RLYNFYWLVAVLVALNFLIFLVFSKWY-VYKEKRLAEV 576

BLAST of MC08g2452 vs. ExPASy Swiss-Prot
Match: Q9LVE0 (Protein NRT1/ PTR FAMILY 6.4 OS=Arabidopsis thaliana OX=3702 GN=NPF6.4 PE=1 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 1.5e-146
Identity = 285/554 (51.44%), Postives = 378/554 (68.23%), Query Frame = 0

Query: 11  DAVDYKGCPASRSKTGGWVPAALILGIELCERLSTMGIAVNLVTYLVGTLHLPSAASANI 70
           +A DY+G P  +SKTGGW+ A LILG EL ER+  MGI++NLVTYLVG LH+ SA SA I
Sbjct: 16  EAYDYRGNPPDKSKTGGWLGAGLILGSELSERICVMGISMNLVTYLVGDLHISSAKSATI 75

Query: 71  VTDFMGTSFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLQQLHPPPCDP- 130
           VT+FMGT  LL LLGGFLAD+ LGRYK +AI AS+  LG   L V T +  + PP CD  
Sbjct: 76  VTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASVTALGVLLLTVATTISSMRPPICDDF 135

Query: 131 -NASKKCKQANGFQMGMLYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFN 190
                +C +ANG Q+ +LY+ LY IALG GGIKS+VSGFG+DQFD  D KE+ QM +FFN
Sbjct: 136 RRLHHQCIEANGHQLALLYVALYTIALGGGGIKSNVSGFGSDQFDTSDPKEEKQMIFFFN 195

Query: 191 RFFLFVSSGTLLAVTVLVYLQDEVGRSWGYGICSVSMFTAILSFLRGTKRYRYKKSMGSP 250
           RF+  +S G+L AV  LVY+QD VGR WGYGI + +M  A +  L GTKRYR+KK  GSP
Sbjct: 196 RFYFSISVGSLFAVIALVYVQDNVGRGWGYGISAATMVVAAIVLLCGTKRYRFKKPKGSP 255

Query: 251 IVHISQVIVAAINKRKMALPFNDALLYEDSAATSRIDHTNQFRFLDKAAIF------AEG 310
              I +V   A  KRK + P + +LL  +    + + HT   + LDKAAI       +  
Sbjct: 256 FTTIWRVGFLAWKKRKESYPAHPSLL--NGYDNTTVPHTEMLKCLDKAAISKNESSPSSK 315

Query: 311 DFEKSVNSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTAYAQMITFSVEQASTMER 370
           DFE+      +PW + +VT+VEEVK++M+L+PIWAT I+FWT Y+QM TF+VEQA+ M+R
Sbjct: 316 DFEEK-----DPWIVSTVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTVEQATFMDR 375

Query: 371 SFGAFQIPAGSLTVFFVTAILITLALYDRLIMPLWRKWKGKP-GFTNLQRIAIGLILSTL 430
             G+F +PAGS + F +  IL+  +L +R+ +PL R+   KP G T+LQRI +GL+ S  
Sbjct: 376 KLGSFTVPAGSYSAFLILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIGVGLVFSMA 435

Query: 431 GMAAAALAETKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSP 490
            MA AA+ E  R   A    +      +S F L+PQ+FLVG+GEAF Y GQL+FFI ++P
Sbjct: 436 AMAVAAVIENARREAAVNNDKK-----ISAFWLVPQYFLVGAGEAFAYVGQLEFFIREAP 495

Query: 491 KGMKTVSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLTI 550
           + MK++STGLFL+T+S+GFFVSS LV++V RVT     + WL  N+N ARL+ FY LL +
Sbjct: 496 ERMKSMSTGLFLSTISMGFFVSSLLVSLVDRVT----DKSWLRSNLNKARLNYFYWLLVV 553

Query: 551 LSAINFVVFLVCAI 556
           L A+NF++F+V A+
Sbjct: 556 LGALNFLIFIVFAM 553

BLAST of MC08g2452 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 5.7e-117
Identity = 239/559 (42.75%), Postives = 339/559 (60.64%), Query Frame = 0

Query: 12  AVDYKGCPASRSKTGGWVPAALILGIELCERLSTMGIAVNLVTYLVGTLHLPSAASANIV 71
           +VD+ G P  + KTG W     ILG E CERL+  GIA NL+TYL   LH  + ++A  V
Sbjct: 29  SVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVSAATNV 88

Query: 72  TDFMGTSFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLQQLHPPPCDPNA 131
           T + GT +L  L+G  LAD++ GRY TIA F+ I  +G   L +   +  L P  C    
Sbjct: 89  TTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAEC---I 148

Query: 132 SKKCKQANGFQMGMLYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFF 191
              C  A   Q  M +  LYLIALGTGGIK  VS FG DQFD+ D +E+ + A FFN F+
Sbjct: 149 GDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASFFNWFY 208

Query: 192 LFVSSGTLLAVTVLVYLQDEVGRSWGYGICSVSMFTAILSFLRGTKRYRYKKSMGSPIVH 251
             ++ G L++ ++LV++Q+  G   G+GI +V M  AI SF  GT  YR++K  GSPI  
Sbjct: 209 FSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGGSPITR 268

Query: 252 ISQVIVAAINKRKMALPFNDALLYEDS------AATSRIDHTNQFRFLDKAAIFAEGDFE 311
           ISQV+VA+  K  + +P +  LLYE        A + +I+HT+  ++LDKAA+ +E   E
Sbjct: 269 ISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVISEE--E 328

Query: 312 KSVNSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTAYAQMITFSVEQASTMERSFG 371
                  N W+LC+VT+VEE+K+++R+ PIWA+ IIF   YAQM T  V+Q   M    G
Sbjct: 329 SKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNCKIG 388

Query: 372 AFQIPAGSLTVFFVTAILITLALYDRLIMPLWRKWKG-KPGFTNLQRIAIGLILSTLGMA 431
           +FQ+P  +L  F   +++I + LYDR I+PL RK+ G   GFT +QR+ IGL +S L MA
Sbjct: 389 SFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSVLCMA 448

Query: 432 AAALAETKRLSVAKAVG--RSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPK 491
           AAA+ E  RL +A  +G   S A +P+SV   IPQ+F++G+ E F + GQL+FF  QSP 
Sbjct: 449 AAAIVEIIRLHMANDLGLVESGAPVPISVLWQIPQYFILGAAEVFYFIGQLEFFYDQSPD 508

Query: 492 GMKTVSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLTIL 551
            M+++ + L L T +LG ++SS ++ +V   T     +GW++DN+N   LD F+ LL  L
Sbjct: 509 AMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDNLNSGHLDYFFWLLAGL 568

Query: 552 SAINFVVFLVCAIWYKPQK 562
           S +N  V+   A  YK +K
Sbjct: 569 SLVNMAVYFFSAARYKQKK 582

BLAST of MC08g2452 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 1.1e-115
Identity = 236/570 (41.40%), Postives = 345/570 (60.53%), Query Frame = 0

Query: 1   MDEKQSLTVTDAVDYKGCPASRSKTGGWVPAALILGIELCERLSTMGIAVNLVTYLVGTL 60
           M+EK   T    VD    PA++ KTG W     ILG E CERL+  G+  NLV YL   L
Sbjct: 1   MEEKDVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRL 60

Query: 61  HLPSAASANIVTDFMGTSFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLQ 120
           +  +A +AN VT++ GT ++  L+G F+AD++LGRY TIA F  I   G   L +   + 
Sbjct: 61  NQGNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVP 120

Query: 121 QLHPPPCDPNASKKCKQANGFQMGMLYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK 180
            L P  C+ +        N  Q  + ++ LY+IALGTGGIK  VS FG DQFDE D+ EK
Sbjct: 121 GLKPGNCNADTC----HPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEK 180

Query: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWGYGICSVSMFTAILSFLRGTKRYR 240
            + + FFN F+  ++ G L+A TVLV++Q  VG  WG+G+ +V+M  A+  F  G++ YR
Sbjct: 181 IKKSSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYR 240

Query: 241 YKKSMGSPIVHISQVIVAAINKRKMALPFNDALLYEDSAATSRID------HTNQFRFLD 300
            ++  GSP+  I QVIVAA  K  + +P + +LL+E +   S I       HT+  +F D
Sbjct: 241 LQRPGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFD 300

Query: 301 KAAIFAEGDFEKSVNSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTAYAQMITFSV 360
           KAA+ ++ D  K  +   NPW+LCSVT+VEE+K ++ LLP+WAT I+F T Y+QM T  V
Sbjct: 301 KAAVESQSDSIK--DGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFV 360

Query: 361 EQASTMERSFGA-FQIPAGSLTVFFVTAILITLALYDRLIMPLWRKW-KGKPGFTNLQRI 420
            Q +TM++  G  F+IP+ SL++F   ++L    +YD+ I+PL RK+ + + GFT LQR+
Sbjct: 361 LQGNTMDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRM 420

Query: 421 AIGLILSTLGMAAAALAETKRLSVAKAVGR-STATLPLSVFLLIPQFFLVGSGEAFIYTG 480
            IGL++S   M  A + E  RL   K         + +S+F  IPQ+ L+G  E F + G
Sbjct: 421 GIGLVVSIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIG 480

Query: 481 QLDFFITQSPKGMKTVSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYAR 540
           QL+FF  Q+P  M+++ + L LTT++LG ++S+ LV VV ++T      GW+ DN+N   
Sbjct: 481 QLEFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGH 540

Query: 541 LDCFYGLLTILSAINFVVFLVCAIWYKPQK 562
           LD F+ LL  LS +NF+V+L  +  YK +K
Sbjct: 541 LDYFFYLLATLSFLNFLVYLWISKRYKYKK 564

BLAST of MC08g2452 vs. NCBI nr
Match: XP_022143087.1 (protein NRT1/ PTR FAMILY 6.2-like isoform X1 [Momordica charantia])

HSP 1 Score: 1133 bits (2930), Expect = 0.0
Identity = 581/581 (100.00%), Postives = 581/581 (100.00%), Query Frame = 0

Query: 1   MDEKQSLTVTDAVDYKGCPASRSKTGGWVPAALILGIELCERLSTMGIAVNLVTYLVGTL 60
           MDEKQSLTVTDAVDYKGCPASRSKTGGWVPAALILGIELCERLSTMGIAVNLVTYLVGTL
Sbjct: 1   MDEKQSLTVTDAVDYKGCPASRSKTGGWVPAALILGIELCERLSTMGIAVNLVTYLVGTL 60

Query: 61  HLPSAASANIVTDFMGTSFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLQ 120
           HLPSAASANIVTDFMGTSFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLQ
Sbjct: 61  HLPSAASANIVTDFMGTSFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLQ 120

Query: 121 QLHPPPCDPNASKKCKQANGFQMGMLYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK 180
           QLHPPPCDPNASKKCKQANGFQMGMLYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK
Sbjct: 121 QLHPPPCDPNASKKCKQANGFQMGMLYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK 180

Query: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWGYGICSVSMFTAILSFLRGTKRYR 240
           AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWGYGICSVSMFTAILSFLRGTKRYR
Sbjct: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWGYGICSVSMFTAILSFLRGTKRYR 240

Query: 241 YKKSMGSPIVHISQVIVAAINKRKMALPFNDALLYEDSAATSRIDHTNQFRFLDKAAIFA 300
           YKKSMGSPIVHISQVIVAAINKRKMALPFNDALLYEDSAATSRIDHTNQFRFLDKAAIFA
Sbjct: 241 YKKSMGSPIVHISQVIVAAINKRKMALPFNDALLYEDSAATSRIDHTNQFRFLDKAAIFA 300

Query: 301 EGDFEKSVNSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTAYAQMITFSVEQASTM 360
           EGDFEKSVNSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTAYAQMITFSVEQASTM
Sbjct: 301 EGDFEKSVNSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTAYAQMITFSVEQASTM 360

Query: 361 ERSFGAFQIPAGSLTVFFVTAILITLALYDRLIMPLWRKWKGKPGFTNLQRIAIGLILST 420
           ERSFGAFQIPAGSLTVFFVTAILITLALYDRLIMPLWRKWKGKPGFTNLQRIAIGLILST
Sbjct: 361 ERSFGAFQIPAGSLTVFFVTAILITLALYDRLIMPLWRKWKGKPGFTNLQRIAIGLILST 420

Query: 421 LGMAAAALAETKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 480
           LGMAAAALAETKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS
Sbjct: 421 LGMAAAALAETKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 480

Query: 481 PKGMKTVSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLT 540
           PKGMKTVSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLT
Sbjct: 481 PKGMKTVSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLT 540

Query: 541 ILSAINFVVFLVCAIWYKPQKPKQLLEMPTSTNEGSVAEKC 581
           ILSAINFVVFLVCAIWYKPQKPKQLLEMPTSTNEGSVAEKC
Sbjct: 541 ILSAINFVVFLVCAIWYKPQKPKQLLEMPTSTNEGSVAEKC 581

BLAST of MC08g2452 vs. NCBI nr
Match: XP_022946201.1 (protein NRT1/ PTR FAMILY 6.2-like [Cucurbita moschata])

HSP 1 Score: 1034 bits (2673), Expect = 0.0
Identity = 526/581 (90.53%), Postives = 548/581 (94.32%), Query Frame = 0

Query: 1   MDEKQSLTVTDAVDYKGCPASRSKTGGWVPAALILGIELCERLSTMGIAVNLVTYLVGTL 60
           MDEKQSLTV+DAVDYKG PA+RSKTGGWVPAALI+GIELCERLSTMGIAVNLVTYLVGTL
Sbjct: 1   MDEKQSLTVSDAVDYKGFPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 60

Query: 61  HLPSAASANIVTDFMGTSFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLQ 120
           HLPSA SANIVTDFMGT FLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKL 
Sbjct: 61  HLPSATSANIVTDFMGTCFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP 120

Query: 121 QLHPPPCDPNASKKCKQANGFQMGMLYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK 180
           QLHPPPCDP+AS+ CKQANGFQMGM+YLPLYLIALGTGG+KSSVSGFGTDQFDEK+DKEK
Sbjct: 121 QLHPPPCDPSASQNCKQANGFQMGMIYLPLYLIALGTGGLKSSVSGFGTDQFDEKNDKEK 180

Query: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWGYGICSVSMFTAILSFLRGTKRYR 240
           AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSW YGICSVSMFTA++ FL GTKRYR
Sbjct: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAVMIFLCGTKRYR 240

Query: 241 YKKSMGSPIVHISQVIVAAINKRKMALPFNDALLYEDSAATSRIDHTNQFRFLDKAAIFA 300
           YKKSMGSPIVHI QVIVAAINKRKM LP NDA+LYED AATS +DHTNQF+ LDKAAI A
Sbjct: 241 YKKSMGSPIVHIFQVIVAAINKRKMELPHNDAMLYEDPAATSTVDHTNQFQLLDKAAIVA 300

Query: 301 EGDFEKSVNSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTAYAQMITFSVEQASTM 360
           EGDFEKSV SA NPWKLCSVTRVEEVKMM RLLPIWATTIIFWT YAQMITFSV QASTM
Sbjct: 301 EGDFEKSVTSAHNPWKLCSVTRVEEVKMMTRLLPIWATTIIFWTTYAQMITFSVVQASTM 360

Query: 361 ERSFGAFQIPAGSLTVFFVTAILITLALYDRLIMPLWRKWKGKPGFTNLQRIAIGLILST 420
           ERS GAFQIPAGSLTVFFV AILITL  YDRL+MPLW+KWKG+PGFTNLQ+IAIGLILST
Sbjct: 361 ERSLGAFQIPAGSLTVFFVAAILITLTFYDRLVMPLWKKWKGQPGFTNLQKIAIGLILST 420

Query: 421 LGMAAAALAETKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 480
           LGMAAAAL E KRLSVAKAVGRSTATLPLSVFLLIPQFFLVGS EAFIYTGQLDFFITQS
Sbjct: 421 LGMAAAALVEMKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSAEAFIYTGQLDFFITQS 480

Query: 481 PKGMKTVSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLT 540
           PKGMK++STGLFLTTLSLGFF+SSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLL 
Sbjct: 481 PKGMKSLSTGLFLTTLSLGFFLSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLA 540

Query: 541 ILSAINFVVFLVCAIWYKPQKPKQLLEMPTSTNEGSVAEKC 581
            LSAINFV FLVCAIWYKPQKPKQ+L M T+TNEGS AEKC
Sbjct: 541 TLSAINFVAFLVCAIWYKPQKPKQVLAMETTTNEGSAAEKC 581

BLAST of MC08g2452 vs. NCBI nr
Match: KAG6599405.1 (Protein NRT1/ PTR FAMILY 6.2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1034 bits (2673), Expect = 0.0
Identity = 525/581 (90.36%), Postives = 549/581 (94.49%), Query Frame = 0

Query: 1   MDEKQSLTVTDAVDYKGCPASRSKTGGWVPAALILGIELCERLSTMGIAVNLVTYLVGTL 60
           MDEKQSLTV+DAVDYKG PA+RSKTGGWVPAALI+GIELCERLSTMGIAVNLVTYLVGTL
Sbjct: 1   MDEKQSLTVSDAVDYKGFPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 60

Query: 61  HLPSAASANIVTDFMGTSFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLQ 120
           HLPSA SANIVTDFMGT FLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKL 
Sbjct: 61  HLPSATSANIVTDFMGTCFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP 120

Query: 121 QLHPPPCDPNASKKCKQANGFQMGMLYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK 180
           QLHPPPCDP+AS+ CKQANGFQMGM+YLPLYLIALGTGG+KSSVSGFGTDQFDEK+DKEK
Sbjct: 121 QLHPPPCDPSASQNCKQANGFQMGMIYLPLYLIALGTGGLKSSVSGFGTDQFDEKNDKEK 180

Query: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWGYGICSVSMFTAILSFLRGTKRYR 240
           AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSW YGICSVSMFTA++ FL GTKRYR
Sbjct: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAVMIFLCGTKRYR 240

Query: 241 YKKSMGSPIVHISQVIVAAINKRKMALPFNDALLYEDSAATSRIDHTNQFRFLDKAAIFA 300
           YKKSMGSPIVHI QVIVAAINKRKM LP NDA+LYED AATS +DHTNQF+ LDKAAI A
Sbjct: 241 YKKSMGSPIVHIFQVIVAAINKRKMELPHNDAMLYEDPAATSTVDHTNQFQLLDKAAIVA 300

Query: 301 EGDFEKSVNSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTAYAQMITFSVEQASTM 360
           EGDFEKSV+SA NPWKLCSVTRVEEVKMM RLLPIWATTIIFWT YAQMITFSV QASTM
Sbjct: 301 EGDFEKSVSSAHNPWKLCSVTRVEEVKMMTRLLPIWATTIIFWTTYAQMITFSVVQASTM 360

Query: 361 ERSFGAFQIPAGSLTVFFVTAILITLALYDRLIMPLWRKWKGKPGFTNLQRIAIGLILST 420
           ERS GAFQIPAGSLTVFFV AILITL  YDRL+MPLW+KWKG+PGFTNLQ+IAIGLILST
Sbjct: 361 ERSLGAFQIPAGSLTVFFVAAILITLTFYDRLVMPLWKKWKGQPGFTNLQKIAIGLILST 420

Query: 421 LGMAAAALAETKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 480
           LGMAAAAL E KRLSVAKAVGRSTATLPLSVFLLIPQFFLVGS EAFIYTGQLDFFITQS
Sbjct: 421 LGMAAAALVEMKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSAEAFIYTGQLDFFITQS 480

Query: 481 PKGMKTVSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLT 540
           PKGMK++STGLFLTTLSLGFF+SSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLL 
Sbjct: 481 PKGMKSLSTGLFLTTLSLGFFLSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLA 540

Query: 541 ILSAINFVVFLVCAIWYKPQKPKQLLEMPTSTNEGSVAEKC 581
            LSAINFV FL+CAIWYKPQKPKQ+L M T+TNEGS AEKC
Sbjct: 541 TLSAINFVAFLICAIWYKPQKPKQVLAMETTTNEGSAAEKC 581

BLAST of MC08g2452 vs. NCBI nr
Match: KAG7030392.1 (Protein NRT1/ PTR FAMILY 6.2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1033 bits (2670), Expect = 0.0
Identity = 525/581 (90.36%), Postives = 548/581 (94.32%), Query Frame = 0

Query: 1   MDEKQSLTVTDAVDYKGCPASRSKTGGWVPAALILGIELCERLSTMGIAVNLVTYLVGTL 60
           MDEKQSLTV+DAVDYKG PA+RSKTGGWVPAALI+GIELCERLSTMGIAVNLVTYLVGTL
Sbjct: 1   MDEKQSLTVSDAVDYKGFPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 60

Query: 61  HLPSAASANIVTDFMGTSFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLQ 120
           HLPSA SANIVTDFMGT FLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKL 
Sbjct: 61  HLPSATSANIVTDFMGTCFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP 120

Query: 121 QLHPPPCDPNASKKCKQANGFQMGMLYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK 180
           QLHPPPCDP+AS+ CKQANGFQMGM+YLPLYLIALGTGG+KSSVSGFGTDQFDEK+DKEK
Sbjct: 121 QLHPPPCDPSASQNCKQANGFQMGMIYLPLYLIALGTGGLKSSVSGFGTDQFDEKNDKEK 180

Query: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWGYGICSVSMFTAILSFLRGTKRYR 240
           AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSW YGICSVSMFTA++ FL GTKRYR
Sbjct: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAVMIFLCGTKRYR 240

Query: 241 YKKSMGSPIVHISQVIVAAINKRKMALPFNDALLYEDSAATSRIDHTNQFRFLDKAAIFA 300
           YKKSMGSPIVHI QVIVAAINKRKM LP NDA+LYED AATS +DHTNQF+ LDKAAI A
Sbjct: 241 YKKSMGSPIVHIFQVIVAAINKRKMELPHNDAMLYEDPAATSTVDHTNQFQLLDKAAIVA 300

Query: 301 EGDFEKSVNSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTAYAQMITFSVEQASTM 360
           EGDFEKSV+SA NPWKLCSVTRVEEVKMM RLLPIWATTIIFWT YAQMITFSV QASTM
Sbjct: 301 EGDFEKSVSSAHNPWKLCSVTRVEEVKMMTRLLPIWATTIIFWTTYAQMITFSVVQASTM 360

Query: 361 ERSFGAFQIPAGSLTVFFVTAILITLALYDRLIMPLWRKWKGKPGFTNLQRIAIGLILST 420
           ERS GAFQIPAGSLTVFFV AILITL  YDRL+MPLW+KWKG+PGFTNLQ+IAIGLILST
Sbjct: 361 ERSLGAFQIPAGSLTVFFVAAILITLTFYDRLVMPLWKKWKGQPGFTNLQKIAIGLILST 420

Query: 421 LGMAAAALAETKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 480
           LGMAAAAL E KRLSVAK VGRSTATLPLSVFLLIPQFFLVGS EAFIYTGQLDFFITQS
Sbjct: 421 LGMAAAALVEMKRLSVAKVVGRSTATLPLSVFLLIPQFFLVGSAEAFIYTGQLDFFITQS 480

Query: 481 PKGMKTVSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLT 540
           PKGMK++STGLFLTTLSLGFF+SSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLL 
Sbjct: 481 PKGMKSLSTGLFLTTLSLGFFLSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLA 540

Query: 541 ILSAINFVVFLVCAIWYKPQKPKQLLEMPTSTNEGSVAEKC 581
            LSAINFV FLVCAIWYKPQKPKQ+L M T+TNEGS AEKC
Sbjct: 541 TLSAINFVAFLVCAIWYKPQKPKQVLAMETTTNEGSAAEKC 581

BLAST of MC08g2452 vs. NCBI nr
Match: XP_023521485.1 (protein NRT1/ PTR FAMILY 6.2-like [Cucurbita pepo subsp. pepo] >XP_023521801.1 protein NRT1/ PTR FAMILY 6.2-like [Cucurbita pepo subsp. pepo] >XP_023547046.1 protein NRT1/ PTR FAMILY 6.2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1032 bits (2668), Expect = 0.0
Identity = 525/581 (90.36%), Postives = 547/581 (94.15%), Query Frame = 0

Query: 1   MDEKQSLTVTDAVDYKGCPASRSKTGGWVPAALILGIELCERLSTMGIAVNLVTYLVGTL 60
           MDEKQSLTV+DAVDYKG PA+RSKTGGWVPAALI+GIELCERLSTMGIAVNLVTYLVGTL
Sbjct: 1   MDEKQSLTVSDAVDYKGFPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 60

Query: 61  HLPSAASANIVTDFMGTSFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLQ 120
           HLPSA SANIVTDFMGT FLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKL 
Sbjct: 61  HLPSATSANIVTDFMGTCFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP 120

Query: 121 QLHPPPCDPNASKKCKQANGFQMGMLYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK 180
           QLHPPPCDP+AS  CKQANGFQMGM+YLPLY+IALGTGG+KSSVSGFGTDQFDEK+DKEK
Sbjct: 121 QLHPPPCDPSASHNCKQANGFQMGMIYLPLYIIALGTGGLKSSVSGFGTDQFDEKNDKEK 180

Query: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWGYGICSVSMFTAILSFLRGTKRYR 240
           AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSW YGICSVSMFTA+L FL GTKRYR
Sbjct: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAVLIFLCGTKRYR 240

Query: 241 YKKSMGSPIVHISQVIVAAINKRKMALPFNDALLYEDSAATSRIDHTNQFRFLDKAAIFA 300
           YKKSMGSPIVHI QVIVAAINKRKM LP NDA+LYED AATS +DHTNQF+ LDKAAI A
Sbjct: 241 YKKSMGSPIVHIFQVIVAAINKRKMELPHNDAMLYEDPAATSTVDHTNQFQLLDKAAIVA 300

Query: 301 EGDFEKSVNSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTAYAQMITFSVEQASTM 360
           EGDFEKSV+SA NPWKLCSVTRVEEVKMM RLLPIWATTIIFWT YAQMITFSV QASTM
Sbjct: 301 EGDFEKSVSSAHNPWKLCSVTRVEEVKMMTRLLPIWATTIIFWTTYAQMITFSVVQASTM 360

Query: 361 ERSFGAFQIPAGSLTVFFVTAILITLALYDRLIMPLWRKWKGKPGFTNLQRIAIGLILST 420
           ERS GAFQIPAGSLTVFFV AILITL  YDRL+MPLW+KWKG+PGFTNLQ+IAIGLILST
Sbjct: 361 ERSLGAFQIPAGSLTVFFVAAILITLTFYDRLVMPLWKKWKGQPGFTNLQKIAIGLILST 420

Query: 421 LGMAAAALAETKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 480
           LGMAAAAL E KRLSVAKAVGRST TLPLSVFLLIPQFFLVGS EAFIYTGQLDFFITQS
Sbjct: 421 LGMAAAALVEMKRLSVAKAVGRSTGTLPLSVFLLIPQFFLVGSAEAFIYTGQLDFFITQS 480

Query: 481 PKGMKTVSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLT 540
           PKGMK++STGLFLTTLSLGFF+SSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLL 
Sbjct: 481 PKGMKSLSTGLFLTTLSLGFFLSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLA 540

Query: 541 ILSAINFVVFLVCAIWYKPQKPKQLLEMPTSTNEGSVAEKC 581
            LSAINFV FLVCAIWYKPQKPKQ+L M T+TNEGS AEKC
Sbjct: 541 TLSAINFVAFLVCAIWYKPQKPKQVLAMETTTNEGSAAEKC 581

BLAST of MC08g2452 vs. ExPASy TrEMBL
Match: A0A6J1CPS2 (protein NRT1/ PTR FAMILY 6.2-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013042 PE=3 SV=1)

HSP 1 Score: 1133 bits (2930), Expect = 0.0
Identity = 581/581 (100.00%), Postives = 581/581 (100.00%), Query Frame = 0

Query: 1   MDEKQSLTVTDAVDYKGCPASRSKTGGWVPAALILGIELCERLSTMGIAVNLVTYLVGTL 60
           MDEKQSLTVTDAVDYKGCPASRSKTGGWVPAALILGIELCERLSTMGIAVNLVTYLVGTL
Sbjct: 1   MDEKQSLTVTDAVDYKGCPASRSKTGGWVPAALILGIELCERLSTMGIAVNLVTYLVGTL 60

Query: 61  HLPSAASANIVTDFMGTSFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLQ 120
           HLPSAASANIVTDFMGTSFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLQ
Sbjct: 61  HLPSAASANIVTDFMGTSFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLQ 120

Query: 121 QLHPPPCDPNASKKCKQANGFQMGMLYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK 180
           QLHPPPCDPNASKKCKQANGFQMGMLYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK
Sbjct: 121 QLHPPPCDPNASKKCKQANGFQMGMLYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK 180

Query: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWGYGICSVSMFTAILSFLRGTKRYR 240
           AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWGYGICSVSMFTAILSFLRGTKRYR
Sbjct: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWGYGICSVSMFTAILSFLRGTKRYR 240

Query: 241 YKKSMGSPIVHISQVIVAAINKRKMALPFNDALLYEDSAATSRIDHTNQFRFLDKAAIFA 300
           YKKSMGSPIVHISQVIVAAINKRKMALPFNDALLYEDSAATSRIDHTNQFRFLDKAAIFA
Sbjct: 241 YKKSMGSPIVHISQVIVAAINKRKMALPFNDALLYEDSAATSRIDHTNQFRFLDKAAIFA 300

Query: 301 EGDFEKSVNSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTAYAQMITFSVEQASTM 360
           EGDFEKSVNSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTAYAQMITFSVEQASTM
Sbjct: 301 EGDFEKSVNSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTAYAQMITFSVEQASTM 360

Query: 361 ERSFGAFQIPAGSLTVFFVTAILITLALYDRLIMPLWRKWKGKPGFTNLQRIAIGLILST 420
           ERSFGAFQIPAGSLTVFFVTAILITLALYDRLIMPLWRKWKGKPGFTNLQRIAIGLILST
Sbjct: 361 ERSFGAFQIPAGSLTVFFVTAILITLALYDRLIMPLWRKWKGKPGFTNLQRIAIGLILST 420

Query: 421 LGMAAAALAETKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 480
           LGMAAAALAETKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS
Sbjct: 421 LGMAAAALAETKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 480

Query: 481 PKGMKTVSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLT 540
           PKGMKTVSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLT
Sbjct: 481 PKGMKTVSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLT 540

Query: 541 ILSAINFVVFLVCAIWYKPQKPKQLLEMPTSTNEGSVAEKC 581
           ILSAINFVVFLVCAIWYKPQKPKQLLEMPTSTNEGSVAEKC
Sbjct: 541 ILSAINFVVFLVCAIWYKPQKPKQLLEMPTSTNEGSVAEKC 581

BLAST of MC08g2452 vs. ExPASy TrEMBL
Match: A0A6J1G2Z7 (protein NRT1/ PTR FAMILY 6.2-like OS=Cucurbita moschata OX=3662 GN=LOC111450357 PE=3 SV=1)

HSP 1 Score: 1034 bits (2673), Expect = 0.0
Identity = 526/581 (90.53%), Postives = 548/581 (94.32%), Query Frame = 0

Query: 1   MDEKQSLTVTDAVDYKGCPASRSKTGGWVPAALILGIELCERLSTMGIAVNLVTYLVGTL 60
           MDEKQSLTV+DAVDYKG PA+RSKTGGWVPAALI+GIELCERLSTMGIAVNLVTYLVGTL
Sbjct: 1   MDEKQSLTVSDAVDYKGFPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 60

Query: 61  HLPSAASANIVTDFMGTSFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLQ 120
           HLPSA SANIVTDFMGT FLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKL 
Sbjct: 61  HLPSATSANIVTDFMGTCFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP 120

Query: 121 QLHPPPCDPNASKKCKQANGFQMGMLYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK 180
           QLHPPPCDP+AS+ CKQANGFQMGM+YLPLYLIALGTGG+KSSVSGFGTDQFDEK+DKEK
Sbjct: 121 QLHPPPCDPSASQNCKQANGFQMGMIYLPLYLIALGTGGLKSSVSGFGTDQFDEKNDKEK 180

Query: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWGYGICSVSMFTAILSFLRGTKRYR 240
           AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSW YGICSVSMFTA++ FL GTKRYR
Sbjct: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAVMIFLCGTKRYR 240

Query: 241 YKKSMGSPIVHISQVIVAAINKRKMALPFNDALLYEDSAATSRIDHTNQFRFLDKAAIFA 300
           YKKSMGSPIVHI QVIVAAINKRKM LP NDA+LYED AATS +DHTNQF+ LDKAAI A
Sbjct: 241 YKKSMGSPIVHIFQVIVAAINKRKMELPHNDAMLYEDPAATSTVDHTNQFQLLDKAAIVA 300

Query: 301 EGDFEKSVNSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTAYAQMITFSVEQASTM 360
           EGDFEKSV SA NPWKLCSVTRVEEVKMM RLLPIWATTIIFWT YAQMITFSV QASTM
Sbjct: 301 EGDFEKSVTSAHNPWKLCSVTRVEEVKMMTRLLPIWATTIIFWTTYAQMITFSVVQASTM 360

Query: 361 ERSFGAFQIPAGSLTVFFVTAILITLALYDRLIMPLWRKWKGKPGFTNLQRIAIGLILST 420
           ERS GAFQIPAGSLTVFFV AILITL  YDRL+MPLW+KWKG+PGFTNLQ+IAIGLILST
Sbjct: 361 ERSLGAFQIPAGSLTVFFVAAILITLTFYDRLVMPLWKKWKGQPGFTNLQKIAIGLILST 420

Query: 421 LGMAAAALAETKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 480
           LGMAAAAL E KRLSVAKAVGRSTATLPLSVFLLIPQFFLVGS EAFIYTGQLDFFITQS
Sbjct: 421 LGMAAAALVEMKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSAEAFIYTGQLDFFITQS 480

Query: 481 PKGMKTVSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLT 540
           PKGMK++STGLFLTTLSLGFF+SSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLL 
Sbjct: 481 PKGMKSLSTGLFLTTLSLGFFLSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLA 540

Query: 541 ILSAINFVVFLVCAIWYKPQKPKQLLEMPTSTNEGSVAEKC 581
            LSAINFV FLVCAIWYKPQKPKQ+L M T+TNEGS AEKC
Sbjct: 541 TLSAINFVAFLVCAIWYKPQKPKQVLAMETTTNEGSAAEKC 581

BLAST of MC08g2452 vs. ExPASy TrEMBL
Match: A0A5A7V7F5 (Protein NRT1/ PTR FAMILY 6.2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G001640 PE=3 SV=1)

HSP 1 Score: 1031 bits (2665), Expect = 0.0
Identity = 530/581 (91.22%), Postives = 543/581 (93.46%), Query Frame = 0

Query: 1   MDEKQSLTVTDAVDYKGCPASRSKTGGWVPAALILGIELCERLSTMGIAVNLVTYLVGTL 60
           MD KQ LTV DAVDYKGCPA+RSKTGGWVPAALI+GIELCERLSTMGIAVNLVTYLVGTL
Sbjct: 1   MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 60

Query: 61  HLPSAASANIVTDFMGTSFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLQ 120
           HLPSA SANIVTDFMGT FLL +LGGFLADSFLGRYKTIAIFA+IQTLGTGTLAVITKL 
Sbjct: 61  HLPSATSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFATIQTLGTGTLAVITKLP 120

Query: 121 QLHPPPCDPNASKKCKQANGFQMGMLYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK 180
           QLHPPPC P  SK CKQANGFQMGM+YLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK
Sbjct: 121 QLHPPPCHPIGSKNCKQANGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK 180

Query: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWGYGICSVSMFTAILSFLRGTKRYR 240
           AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSW YGICSVSMFTAIL FL GTKRYR
Sbjct: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR 240

Query: 241 YKKSMGSPIVHISQVIVAAINKRKMALPFNDALLYEDSAATSRIDHTNQFRFLDKAAIFA 300
           YKKSMGSPIVHI QVIVAAINKR M L  N  LLYEDSAATSRIDHTNQF+FLDKAAI A
Sbjct: 241 YKKSMGSPIVHIFQVIVAAINKRSMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA 300

Query: 301 EGDFEKSVNSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTAYAQMITFSVEQASTM 360
           EGDFEKSV+SAPNPWKLCSVTRVEEVKMMMRLLP+WATTIIFWT YAQMITFSV QASTM
Sbjct: 301 EGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPVWATTIIFWTTYAQMITFSVVQASTM 360

Query: 361 ERSFGAFQIPAGSLTVFFVTAILITLALYDRLIMPLWRKWKGKPGFTNLQRIAIGLILST 420
           ERS G F+IPAGSLTVFFV AILITLA YDRLIMPLW+KWKG+PGFTNLQRIAIGLILST
Sbjct: 361 ERSLGNFKIPAGSLTVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST 420

Query: 421 LGMAAAALAETKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 480
            GMAAAAL E KRLSVAKAVG STATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS
Sbjct: 421 FGMAAAALVEMKRLSVAKAVGISTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 480

Query: 481 PKGMKTVSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLT 540
           PKGMKT+STGLFLTTLSLGFFVSSFLVAVVKRVTGS DGQGWLADNINYARLDCFYGLL 
Sbjct: 481 PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKRVTGSMDGQGWLADNINYARLDCFYGLLA 540

Query: 541 ILSAINFVVFLVCAIWYKPQKPKQLLEMPTSTNEGSVAEKC 581
           ILSAINFV FLVCA+WYKPQKPKQLLEM TSTN GS AEKC
Sbjct: 541 ILSAINFVAFLVCAVWYKPQKPKQLLEMETSTNGGSGAEKC 581

BLAST of MC08g2452 vs. ExPASy TrEMBL
Match: A0A1S3C670 (protein NRT1/ PTR FAMILY 6.2 OS=Cucumis melo OX=3656 GN=LOC103497318 PE=3 SV=1)

HSP 1 Score: 1031 bits (2665), Expect = 0.0
Identity = 530/581 (91.22%), Postives = 543/581 (93.46%), Query Frame = 0

Query: 1   MDEKQSLTVTDAVDYKGCPASRSKTGGWVPAALILGIELCERLSTMGIAVNLVTYLVGTL 60
           MD KQ LTV DAVDYKGCPA+RSKTGGWVPAALI+GIELCERLSTMGIAVNLVTYLVGTL
Sbjct: 1   MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 60

Query: 61  HLPSAASANIVTDFMGTSFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLQ 120
           HLPSA SANIVTDFMGT FLL +LGGFLADSFLGRYKTIAIFA+IQTLGTGTLAVITKL 
Sbjct: 61  HLPSATSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFATIQTLGTGTLAVITKLP 120

Query: 121 QLHPPPCDPNASKKCKQANGFQMGMLYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK 180
           QLHPPPC P  SK CKQANGFQMGM+YLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK
Sbjct: 121 QLHPPPCHPIGSKNCKQANGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK 180

Query: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWGYGICSVSMFTAILSFLRGTKRYR 240
           AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSW YGICSVSMFTAIL FL GTKRYR
Sbjct: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR 240

Query: 241 YKKSMGSPIVHISQVIVAAINKRKMALPFNDALLYEDSAATSRIDHTNQFRFLDKAAIFA 300
           YKKSMGSPIVHI QVIVAAINKR M L  N  LLYEDSAATSRIDHTNQF+FLDKAAI A
Sbjct: 241 YKKSMGSPIVHIFQVIVAAINKRSMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA 300

Query: 301 EGDFEKSVNSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTAYAQMITFSVEQASTM 360
           EGDFEKSV+SAPNPWKLCSVTRVEEVKMMMRLLP+WATTIIFWT YAQMITFSV QASTM
Sbjct: 301 EGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPVWATTIIFWTTYAQMITFSVVQASTM 360

Query: 361 ERSFGAFQIPAGSLTVFFVTAILITLALYDRLIMPLWRKWKGKPGFTNLQRIAIGLILST 420
           ERS G F+IPAGSLTVFFV AILITLA YDRLIMPLW+KWKG+PGFTNLQRIAIGLILST
Sbjct: 361 ERSLGNFKIPAGSLTVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST 420

Query: 421 LGMAAAALAETKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 480
            GMAAAAL E KRLSVAKAVG STATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS
Sbjct: 421 FGMAAAALVEMKRLSVAKAVGISTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 480

Query: 481 PKGMKTVSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLT 540
           PKGMKT+STGLFLTTLSLGFFVSSFLVAVVKRVTGS DGQGWLADNINYARLDCFYGLL 
Sbjct: 481 PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKRVTGSMDGQGWLADNINYARLDCFYGLLA 540

Query: 541 ILSAINFVVFLVCAIWYKPQKPKQLLEMPTSTNEGSVAEKC 581
           ILSAINFV FLVCA+WYKPQKPKQLLEM TSTN GS AEKC
Sbjct: 541 ILSAINFVAFLVCAVWYKPQKPKQLLEMETSTNGGSGAEKC 581

BLAST of MC08g2452 vs. ExPASy TrEMBL
Match: A0A5D3BHY8 (Protein NRT1/ PTR FAMILY 6.2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G00180 PE=3 SV=1)

HSP 1 Score: 1030 bits (2664), Expect = 0.0
Identity = 530/581 (91.22%), Postives = 543/581 (93.46%), Query Frame = 0

Query: 1   MDEKQSLTVTDAVDYKGCPASRSKTGGWVPAALILGIELCERLSTMGIAVNLVTYLVGTL 60
           MD KQ LTV DAVDYKGCPA+RSKTGGWVPAALI+GIELCERLSTMGIAVNLVTYLVGTL
Sbjct: 1   MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 60

Query: 61  HLPSAASANIVTDFMGTSFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLQ 120
           HLPSA SANIVTDFMGT FLL +LGGFLADSFLGRYKTIAIFA+IQTLGTGTLAVITKL 
Sbjct: 61  HLPSATSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFATIQTLGTGTLAVITKLP 120

Query: 121 QLHPPPCDPNASKKCKQANGFQMGMLYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK 180
           QLHPPPC P  SK CKQANGFQMGM+YLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK
Sbjct: 121 QLHPPPCHPIGSKNCKQANGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK 180

Query: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWGYGICSVSMFTAILSFLRGTKRYR 240
           AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSW YGICSVSMFTAIL FL GTKRYR
Sbjct: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR 240

Query: 241 YKKSMGSPIVHISQVIVAAINKRKMALPFNDALLYEDSAATSRIDHTNQFRFLDKAAIFA 300
           YKKSMGSPIVHI QVIVAAINKR M L  N  LLYEDSAATSRIDHTNQF+FLDKAAI A
Sbjct: 241 YKKSMGSPIVHIFQVIVAAINKRTMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA 300

Query: 301 EGDFEKSVNSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTAYAQMITFSVEQASTM 360
           EGDFEKSV+SAPNPWKLCSVTRVEEVKMMMRLLP+WATTIIFWT YAQMITFSV QASTM
Sbjct: 301 EGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPVWATTIIFWTTYAQMITFSVVQASTM 360

Query: 361 ERSFGAFQIPAGSLTVFFVTAILITLALYDRLIMPLWRKWKGKPGFTNLQRIAIGLILST 420
           ERS G F+IPAGSLTVFFV AILITLA YDRLIMPLW+KWKG+PGFTNLQRIAIGLILST
Sbjct: 361 ERSLGNFKIPAGSLTVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST 420

Query: 421 LGMAAAALAETKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 480
            GMAAAAL E KRLSVAKAVG STATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS
Sbjct: 421 FGMAAAALVEMKRLSVAKAVGISTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 480

Query: 481 PKGMKTVSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLT 540
           PKGMKT+STGLFLTTLSLGFFVSSFLVAVVKRVTGS DGQGWLADNINYARLDCFYGLL 
Sbjct: 481 PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKRVTGSMDGQGWLADNINYARLDCFYGLLA 540

Query: 541 ILSAINFVVFLVCAIWYKPQKPKQLLEMPTSTNEGSVAEKC 581
           ILSAINFV FLVCA+WYKPQKPKQLLEM TSTN GS AEKC
Sbjct: 541 ILSAINFVAFLVCAVWYKPQKPKQLLEMETSTNGGSGAEKC 581

BLAST of MC08g2452 vs. TAIR 10
Match: AT2G26690.1 (Major facilitator superfamily protein )

HSP 1 Score: 826.6 bits (2134), Expect = 1.2e-239
Identity = 426/583 (73.07%), Postives = 486/583 (83.36%), Query Frame = 0

Query: 1   MDEKQSLTVTDAVDYKGCPASRSKTGGWVPAALILGIELCERLSTMGIAVNLVTYLVGTL 60
           M+ K S TV DAVDYKG PA +SKTGGW+ AALILGIE+ ERLSTMGIAVNLVTYL+ T+
Sbjct: 1   MESKGSWTVADAVDYKGRPADKSKTGGWITAALILGIEVVERLSTMGIAVNLVTYLMETM 60

Query: 61  HLPSAASANIVTDFMGTSFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLQ 120
           HLPS+ SANIVTDFMGTSFLLCLLGGFLADSFLGR+KTI IF++IQ LGTG LAV TKL 
Sbjct: 61  HLPSSTSANIVTDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTIQALGTGALAVATKLP 120

Query: 121 QLHPPPCDPNASKKCKQANGFQMGMLYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK 180
           +L PP C  +  + C  A  FQM +LY+ LYLIALGTGG+KSS+SGFG+DQFD+KD KEK
Sbjct: 121 ELRPPTC--HHGEACIPATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEK 180

Query: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWGYGICSVSMFTAILSFLRGTKRYR 240
           A MA+FFNRFF F+S GTLLAVTVLVY+QDEVGRSW YGIC+VSM  AI+ FL GTKRYR
Sbjct: 181 AHMAFFFNRFFFFISMGTLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYR 240

Query: 241 YKKSMGSPIVHISQVIVAAINKRKMALPFNDALLYEDSAATSRIDHTNQFRFLDKAAIFA 300
           YKKS GSP+V I QVI AA  KRKM LP +   LYED+    RI+HT+QF  LDKAAI A
Sbjct: 241 YKKSQGSPVVQIFQVIAAAFRKRKMELPQSIVYLYEDNPEGIRIEHTDQFHLLDKAAIVA 300

Query: 301 EGDFEKSVN--SAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTAYAQMITFSVEQAS 360
           EGDFE++++  + PNPWKL SVT+VEEVKMM+RLLPIWATTIIFWT YAQMITFSVEQAS
Sbjct: 301 EGDFEQTLDGVAIPNPWKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQAS 360

Query: 361 TMERSFGAFQIPAGSLTVFFVTAILITLALYDRLIMPLWRKWKGKPGFTNLQRIAIGLIL 420
           TM R+ G+F+IPAGSLTVFFV AILITLA+YDR IMP W+KWKGKPGF++LQRIAIGL+L
Sbjct: 361 TMRRNIGSFKIPAGSLTVFFVAAILITLAVYDRAIMPFWKKWKGKPGFSSLQRIAIGLVL 420

Query: 421 STLGMAAAALAETKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFIT 480
           ST GMAAAAL E KRLSVAK+   S  TLP+SVFLL+PQFFLVG+GEAFIYTGQLDFFIT
Sbjct: 421 STAGMAAAALVEQKRLSVAKS--SSQKTLPISVFLLVPQFFLVGAGEAFIYTGQLDFFIT 480

Query: 481 QSPKGMKTVSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGL 540
           QSPKGMKT+STGLFLTTLSLGFFVSSFLV++VKRVT +    GWLADNIN+ RLD FY L
Sbjct: 481 QSPKGMKTMSTGLFLTTLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHGRLDYFYWL 540

Query: 541 LTILSAINFVVFLVCAIWYKPQKPKQLLEMPTSTNEGSVAEKC 582
           L ILS INFVV+++CA+W+KP K K  +E      +G   E C
Sbjct: 541 LVILSGINFVVYIICALWFKPTKGKDSVE--KENGKGFSVEDC 577

BLAST of MC08g2452 vs. TAIR 10
Match: AT1G12110.1 (nitrate transporter 1.1 )

HSP 1 Score: 585.1 bits (1507), Expect = 6.2e-167
Identity = 310/578 (53.63%), Postives = 407/578 (70.42%), Query Frame = 0

Query: 2   DEKQSLTVTDAVDYKGCPASRSKTGGWVPAALILGIELCERLSTMGIAVNLVTYLVGTLH 61
           + K    + DA D++G PA RSKTGGW  AA+IL IE  ERL+T+GI VNLVTYL GT+H
Sbjct: 5   ETKSDDILLDAWDFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGTMH 64

Query: 62  LPSAASANIVTDFMGTSFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLQQ 121
           L +A +AN VT+F+GTSF+LCLLGGF+AD+FLGRY TIAIFA+IQ  G   L + T +  
Sbjct: 65  LGNATAANTVTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTIIPG 124

Query: 122 LHPPPCDPNASKKCKQANGFQMGMLYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKA 181
           L PP C+P  S  C+QA+G Q+ +LYL LYL ALGTGG+K+SVSGFG+DQFDE + KE++
Sbjct: 125 LRPPRCNPTTSSHCEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERS 184

Query: 182 QMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWGYGICSVSMFTAILSFLRGTKRYRY 241
           +M YFFNRFF  ++ G+LLAVTVLVY+QD+VGR WGYGIC+ ++  A+  FL GT RYR+
Sbjct: 185 KMTYFFNRFFFCINVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRF 244

Query: 242 KKSMGSPIVHISQVIVAAINKRKMALPFNDALLYE---------DSAATSRIDHTNQFRF 301
           KK +GSP+  ++ VIVAA   RK+ LP + + LY+               ++ HT QFR 
Sbjct: 245 KKLIGSPMTQVAAVIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRS 304

Query: 302 LDKAAIFAEGDFEKSVNS-APNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTAYAQMIT 361
           LDKAAI    D E  V S   N W L ++T VEEVK ++R+LPIWAT I+FWT +AQ+ T
Sbjct: 305 LDKAAI---RDQEAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTT 364

Query: 362 FSVEQASTMERSFGAFQIPAGSLTVFFVTAILITLALYDRLIMPLWRKWKGKP-GFTNLQ 421
            SV Q+ T++RS G+F+IP  S+ VF+V  +L+T A+YDR+ + L +K    P G   LQ
Sbjct: 365 LSVAQSETLDRSIGSFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQ 424

Query: 422 RIAIGLILSTLGMAAAALAETKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYT 481
           RI +GL   ++ MA AAL E KRL  A A G +  TLPL  +LLIPQ+ +VG GEA IYT
Sbjct: 425 RIGLGLFFGSMAMAVAALVELKRLRTAHAHGPTVKTLPLGFYLLIPQYLIVGIGEALIYT 484

Query: 482 GQLDFFITQSPKGMKTVSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYA 541
           GQLDFF+ + PKGMK +STGL L+TL+LGFF SS LV +V++ TG      W+AD++N  
Sbjct: 485 GQLDFFLRECPKGMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGK--AHPWIADDLNKG 544

Query: 542 RLDCFYGLLTILSAINFVVFLVCAIWYKPQKPKQLLEM 569
           RL  FY L+ +L A+NF++FLV + WY   K K+L E+
Sbjct: 545 RLYNFYWLVAVLVALNFLIFLVFSKWY-VYKEKRLAEV 576

BLAST of MC08g2452 vs. TAIR 10
Match: AT3G21670.1 (Major facilitator superfamily protein )

HSP 1 Score: 521.2 bits (1341), Expect = 1.1e-147
Identity = 285/554 (51.44%), Postives = 378/554 (68.23%), Query Frame = 0

Query: 11  DAVDYKGCPASRSKTGGWVPAALILGIELCERLSTMGIAVNLVTYLVGTLHLPSAASANI 70
           +A DY+G P  +SKTGGW+ A LILG EL ER+  MGI++NLVTYLVG LH+ SA SA I
Sbjct: 16  EAYDYRGNPPDKSKTGGWLGAGLILGSELSERICVMGISMNLVTYLVGDLHISSAKSATI 75

Query: 71  VTDFMGTSFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLQQLHPPPCDP- 130
           VT+FMGT  LL LLGGFLAD+ LGRYK +AI AS+  LG   L V T +  + PP CD  
Sbjct: 76  VTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASVTALGVLLLTVATTISSMRPPICDDF 135

Query: 131 -NASKKCKQANGFQMGMLYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFN 190
                +C +ANG Q+ +LY+ LY IALG GGIKS+VSGFG+DQFD  D KE+ QM +FFN
Sbjct: 136 RRLHHQCIEANGHQLALLYVALYTIALGGGGIKSNVSGFGSDQFDTSDPKEEKQMIFFFN 195

Query: 191 RFFLFVSSGTLLAVTVLVYLQDEVGRSWGYGICSVSMFTAILSFLRGTKRYRYKKSMGSP 250
           RF+  +S G+L AV  LVY+QD VGR WGYGI + +M  A +  L GTKRYR+KK  GSP
Sbjct: 196 RFYFSISVGSLFAVIALVYVQDNVGRGWGYGISAATMVVAAIVLLCGTKRYRFKKPKGSP 255

Query: 251 IVHISQVIVAAINKRKMALPFNDALLYEDSAATSRIDHTNQFRFLDKAAIF------AEG 310
              I +V   A  KRK + P + +LL  +    + + HT   + LDKAAI       +  
Sbjct: 256 FTTIWRVGFLAWKKRKESYPAHPSLL--NGYDNTTVPHTEMLKCLDKAAISKNESSPSSK 315

Query: 311 DFEKSVNSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTAYAQMITFSVEQASTMER 370
           DFE+      +PW + +VT+VEEVK++M+L+PIWAT I+FWT Y+QM TF+VEQA+ M+R
Sbjct: 316 DFEEK-----DPWIVSTVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTVEQATFMDR 375

Query: 371 SFGAFQIPAGSLTVFFVTAILITLALYDRLIMPLWRKWKGKP-GFTNLQRIAIGLILSTL 430
             G+F +PAGS + F +  IL+  +L +R+ +PL R+   KP G T+LQRI +GL+ S  
Sbjct: 376 KLGSFTVPAGSYSAFLILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIGVGLVFSMA 435

Query: 431 GMAAAALAETKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSP 490
            MA AA+ E  R   A    +      +S F L+PQ+FLVG+GEAF Y GQL+FFI ++P
Sbjct: 436 AMAVAAVIENARREAAVNNDKK-----ISAFWLVPQYFLVGAGEAFAYVGQLEFFIREAP 495

Query: 491 KGMKTVSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLTI 550
           + MK++STGLFL+T+S+GFFVSS LV++V RVT     + WL  N+N ARL+ FY LL +
Sbjct: 496 ERMKSMSTGLFLSTISMGFFVSSLLVSLVDRVT----DKSWLRSNLNKARLNYFYWLLVV 553

Query: 551 LSAINFVVFLVCAI 556
           L A+NF++F+V A+
Sbjct: 556 LGALNFLIFIVFAM 553

BLAST of MC08g2452 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 422.9 bits (1086), Expect = 4.1e-118
Identity = 239/559 (42.75%), Postives = 339/559 (60.64%), Query Frame = 0

Query: 12  AVDYKGCPASRSKTGGWVPAALILGIELCERLSTMGIAVNLVTYLVGTLHLPSAASANIV 71
           +VD+ G P  + KTG W     ILG E CERL+  GIA NL+TYL   LH  + ++A  V
Sbjct: 29  SVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVSAATNV 88

Query: 72  TDFMGTSFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLQQLHPPPCDPNA 131
           T + GT +L  L+G  LAD++ GRY TIA F+ I  +G   L +   +  L P  C    
Sbjct: 89  TTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAEC---I 148

Query: 132 SKKCKQANGFQMGMLYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFF 191
              C  A   Q  M +  LYLIALGTGGIK  VS FG DQFD+ D +E+ + A FFN F+
Sbjct: 149 GDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASFFNWFY 208

Query: 192 LFVSSGTLLAVTVLVYLQDEVGRSWGYGICSVSMFTAILSFLRGTKRYRYKKSMGSPIVH 251
             ++ G L++ ++LV++Q+  G   G+GI +V M  AI SF  GT  YR++K  GSPI  
Sbjct: 209 FSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGGSPITR 268

Query: 252 ISQVIVAAINKRKMALPFNDALLYEDS------AATSRIDHTNQFRFLDKAAIFAEGDFE 311
           ISQV+VA+  K  + +P +  LLYE        A + +I+HT+  ++LDKAA+ +E   E
Sbjct: 269 ISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVISEE--E 328

Query: 312 KSVNSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTAYAQMITFSVEQASTMERSFG 371
                  N W+LC+VT+VEE+K+++R+ PIWA+ IIF   YAQM T  V+Q   M    G
Sbjct: 329 SKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNCKIG 388

Query: 372 AFQIPAGSLTVFFVTAILITLALYDRLIMPLWRKWKG-KPGFTNLQRIAIGLILSTLGMA 431
           +FQ+P  +L  F   +++I + LYDR I+PL RK+ G   GFT +QR+ IGL +S L MA
Sbjct: 389 SFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSVLCMA 448

Query: 432 AAALAETKRLSVAKAVG--RSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPK 491
           AAA+ E  RL +A  +G   S A +P+SV   IPQ+F++G+ E F + GQL+FF  QSP 
Sbjct: 449 AAAIVEIIRLHMANDLGLVESGAPVPISVLWQIPQYFILGAAEVFYFIGQLEFFYDQSPD 508

Query: 492 GMKTVSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLTIL 551
            M+++ + L L T +LG ++SS ++ +V   T     +GW++DN+N   LD F+ LL  L
Sbjct: 509 AMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDNLNSGHLDYFFWLLAGL 568

Query: 552 SAINFVVFLVCAIWYKPQK 562
           S +N  V+   A  YK +K
Sbjct: 569 SLVNMAVYFFSAARYKQKK 582

BLAST of MC08g2452 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 418.7 bits (1075), Expect = 7.7e-117
Identity = 236/570 (41.40%), Postives = 345/570 (60.53%), Query Frame = 0

Query: 1   MDEKQSLTVTDAVDYKGCPASRSKTGGWVPAALILGIELCERLSTMGIAVNLVTYLVGTL 60
           M+EK   T    VD    PA++ KTG W     ILG E CERL+  G+  NLV YL   L
Sbjct: 1   MEEKDVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRL 60

Query: 61  HLPSAASANIVTDFMGTSFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLQ 120
           +  +A +AN VT++ GT ++  L+G F+AD++LGRY TIA F  I   G   L +   + 
Sbjct: 61  NQGNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVP 120

Query: 121 QLHPPPCDPNASKKCKQANGFQMGMLYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK 180
            L P  C+ +        N  Q  + ++ LY+IALGTGGIK  VS FG DQFDE D+ EK
Sbjct: 121 GLKPGNCNADTC----HPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEK 180

Query: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWGYGICSVSMFTAILSFLRGTKRYR 240
            + + FFN F+  ++ G L+A TVLV++Q  VG  WG+G+ +V+M  A+  F  G++ YR
Sbjct: 181 IKKSSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYR 240

Query: 241 YKKSMGSPIVHISQVIVAAINKRKMALPFNDALLYEDSAATSRID------HTNQFRFLD 300
            ++  GSP+  I QVIVAA  K  + +P + +LL+E +   S I       HT+  +F D
Sbjct: 241 LQRPGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFD 300

Query: 301 KAAIFAEGDFEKSVNSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTAYAQMITFSV 360
           KAA+ ++ D  K  +   NPW+LCSVT+VEE+K ++ LLP+WAT I+F T Y+QM T  V
Sbjct: 301 KAAVESQSDSIK--DGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFV 360

Query: 361 EQASTMERSFGA-FQIPAGSLTVFFVTAILITLALYDRLIMPLWRKW-KGKPGFTNLQRI 420
            Q +TM++  G  F+IP+ SL++F   ++L    +YD+ I+PL RK+ + + GFT LQR+
Sbjct: 361 LQGNTMDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRM 420

Query: 421 AIGLILSTLGMAAAALAETKRLSVAKAVGR-STATLPLSVFLLIPQFFLVGSGEAFIYTG 480
            IGL++S   M  A + E  RL   K         + +S+F  IPQ+ L+G  E F + G
Sbjct: 421 GIGLVVSIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIG 480

Query: 481 QLDFFITQSPKGMKTVSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYAR 540
           QL+FF  Q+P  M+++ + L LTT++LG ++S+ LV VV ++T      GW+ DN+N   
Sbjct: 481 QLEFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGH 540

Query: 541 LDCFYGLLTILSAINFVVFLVCAIWYKPQK 562
           LD F+ LL  LS +NF+V+L  +  YK +K
Sbjct: 541 LDYFFYLLATLSFLNFLVYLWISKRYKYKK 564