MC08g2448 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g2448
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Pectin lyase-like superfamily protein
Location	MC08: 33288201 .. 33289586 (+)
RNA-Seq Expression	MC08g2448
Synteny	MC08g2448

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGGCAATACTAAAAGCATGGAAAGCAGCATGTAGCTCTAAAACAGAAAGTTCTACAGTGCTAATTCCCAAGAGGCATACACTTCTAGTAAAACCAATTGTTTTGAGAGGTCCATGCAGGGCAAACAGCATCACTTTCATGGTAAAGAAAAAGCAGGGGCTTTCCCCTTTATGATTAAGTCTTCCCTAGATACTACCTGTCTTCAGGTAGATGCAAATTTTCACAGATACATGGAGAGATTGTGGCACCAACCTCACCAGGCGAATGGACGCGGACTGACCAGAGCCAGTGGCTGGCTTTTGAAGGGGTAAGCAGGCTCAATGTCACTGGCACCGGCATTGGCAAAATAGATGGAAGAGGTACAAGATGGTGGAGCCAATCATGTAGGGATCACCCAGACTTGGTAAGTAAATCATCAGATAAGAAAAAGTTTCCAATGCATATAAAGTAATATCCAGTGTCTGATGTTTCTTCTACATTTTTCAGAAGGGTTGCACCTCATTAGCACCTACTGTAAGTATTTTCTGCACAAAGCAATTTTCTAGTATCTGCTAGAGAGAGAGAGAGAGTATTTGGTGAACTTACCAAATCTCGTGCATTATTGCAGGCAATGAAGATTATTTCATGCAACAACACCAGCTTGAGCAACATTCACTTTATTAACAGCTCACAAACACATGTACTAATAATGGGGAGCAAGAGAGTTGAAATTAAAAATTTGGTAATCACAGCTCCAGAAAGCAGCCCAAACACTGATGGCATTCACATTCATTCCTCTCACAATGTTATTATAAAGAACACAACAATAGGCACAGGTAATTTGGATGGAATTATTTCCAGAAAGATCGGCTTTAACAAGATTAACAAGTATCGACGTTTTTAATTAAGAAAATAAATAACATTAATTGTGACGAGCAGGTGATGATTGTGTATCTATAGGAGATTACACCTCTAACGTGAATATCTCACACATCAGATGTGGACCTGGTCATGGAATAAGGTAGGTTCCCTATTCAGATAAGGAACTCCATAAAACACGCTTTAGAACAAGAACACGGAAAAATATAGAACAAAATTCGTTGTTTCAATAGCTCATTTCGTATACTATGTGAACCTGAAAATAGCATTGGAAGCTTGGGAAGAAGCGGCAATTTTGTACAAGTAGAGGATATTAGAGTGAGTAATGTCTACTTCAAAGGAACTACCAATGGAGCTCGGATTAAGACTTGGCAGGTAATGGAAATTCTACTCATTAATTTATAAAATAAACTGTAAAGCTTACACTAACACTTCCAAAAGATTGGTAGGGGTAACATCCGAAGGGTCACATTTCAGAATCTCTATTTTGAATCAGTCAATAACCCCTTTATTATAGACCAAAACTAC

mRNA sequence

CAGGCAATACTAAAAGCATGGAAAGCAGCATGTAGCTCTAAAACAGAAAGTTCTACAGTGCTAATTCCCAAGAGGCATACACTTCTAGTAAAACCAATTGTTTTGAGAGGTAGATGCAAATTTTCACAGATACATGGAGAGATTGTGGCACCAACCTCACCAGGCGAATGGACGCGGACTGACCAGAGCCAGTGGCTGGCTTTTGAAGGGGTAAGCAGGCTCAATGTCACTGGCACCGGCATTGGCAAAATAGATGGAAGAGGTACAAGATGGTGGAGCCAATCATGTAGGGATCACCCAGACTTGAGAGTATTTGGTGAACTTACCAAATCTCGTGCATTATTGCAGGCAATGAAGATTATTTCATGCAACAACACCAGCTTGAGCAACATTCACTTTATTAACAGCTCACAAACACATGTACTAATAATGGGGAGCAAGAGAGTTGAAATTAAAAATTTGGTAATCACAGCTCCAGAAAGCAGCCCAAACACTGATGGCATTCACATTCATTCCTCTCACAATGTTATTATAAAGAACACAACAATAGGCACAGGTGATGATTGTGTATCTATAGGAGATTACACCTCTAACGTGAATATCTCACACATCAGATGTGGACCTGGTCATGGAATAAGCATTGGAAGCTTGGGAAGAAGCGGCAATTTTGTACAAGTAGAGGATATTAGAGTGAGTAATGTCTACTTCAAAGGAACTACCAATGGAGCTCGGATTAAGACTTGGCAGATTGGTAGGGGTAACATCCGAAGGGTCACATTTCAGAATCTCTATTTTGAATCAGTCAATAACCCCTTTATTATAGACCAAAACTAC

Coding sequence (CDS)

CAGGCAATACTAAAAGCATGGAAAGCAGCATGTAGCTCTAAAACAGAAAGTTCTACAGTGCTAATTCCCAAGAGGCATACACTTCTAGTAAAACCAATTGTTTTGAGAGGTAGATGCAAATTTTCACAGATACATGGAGAGATTGTGGCACCAACCTCACCAGGCGAATGGACGCGGACTGACCAGAGCCAGTGGCTGGCTTTTGAAGGGGTAAGCAGGCTCAATGTCACTGGCACCGGCATTGGCAAAATAGATGGAAGAGGTACAAGATGGTGGAGCCAATCATGTAGGGATCACCCAGACTTGAGAGTATTTGGTGAACTTACCAAATCTCGTGCATTATTGCAGGCAATGAAGATTATTTCATGCAACAACACCAGCTTGAGCAACATTCACTTTATTAACAGCTCACAAACACATGTACTAATAATGGGGAGCAAGAGAGTTGAAATTAAAAATTTGGTAATCACAGCTCCAGAAAGCAGCCCAAACACTGATGGCATTCACATTCATTCCTCTCACAATGTTATTATAAAGAACACAACAATAGGCACAGGTGATGATTGTGTATCTATAGGAGATTACACCTCTAACGTGAATATCTCACACATCAGATGTGGACCTGGTCATGGAATAAGCATTGGAAGCTTGGGAAGAAGCGGCAATTTTGTACAAGTAGAGGATATTAGAGTGAGTAATGTCTACTTCAAAGGAACTACCAATGGAGCTCGGATTAAGACTTGGCAGATTGGTAGGGGTAACATCCGAAGGGTCACATTTCAGAATCTCTATTTTGAATCAGTCAATAACCCCTTTATTATAGACCAAAACTAC

Protein sequence

QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGRCKFSQIHGEIVAPTSPGEWTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLRVFGELTKSRALLQAMKIISCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNTTIGTGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTTNGARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNY

Homology

[Close]

BLAST of MC08g2448 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 2.5e-62
Identity = 121/281 (43.06%), Postives = 170/281 (60.50%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGRCKFS---QIHGEIVAPTSPGEW 60
           +A   AWK ACSSK + + +L+P+ +T L++PI L G CK     QI G I+AP  P  W
Sbjct: 70  KAFADAWKTACSSKVK-TRILVPENYTCLLRPIDLSGPCKARLTLQISGTIIAPNDPDVW 129

Query: 61  TRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLRVFGELTKSRALLQA 120
              ++ +WL F G+SRL V G   G ++G G  WW +SC+ +            R    A
Sbjct: 130 EGLNRRKWLYFHGLSRLTVEGG--GTVNGMGQEWWERSCKHN-------HSNPCRGAPTA 189

Query: 121 MKIISCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVI 180
           +    C N  + N++ I+S Q H+ +   +RV I  L + AP +SPNTDGIHI  S  ++
Sbjct: 190 LTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDGIHISVSRGIV 249

Query: 181 IKNTTIGTGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFK 240
           I NTT+ TGDDC+SI   ++ ++IS+I CGPGHGISIGSLG+S ++ +V DI V      
Sbjct: 250 IDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVRDITVDTAIIS 309

Query: 241 GTTNGARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNY 279
            T NG RIKTWQ G G + ++ F+N+   +V+NP IIDQ Y
Sbjct: 310 DTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYY 340

[Close]

BLAST of MC08g2448 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 6.4e-58
Identity = 125/282 (44.33%), Postives = 169/282 (59.93%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGRCK----FSQIHGEIVAPTSPGE 60
           QA LKAW+A CS  T     ++P   T +++P+  +G CK    F Q+ G++VAP S G 
Sbjct: 41  QAFLKAWEAVCSG-TGDGQFVVPAGMTFMLQPLKFQGSCKSTPVFVQMLGKLVAP-SKGN 100

Query: 61  WTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLRVFGELTKSRALLQ 120
           W + D+ QW+ F  +  L + G   G+I+G+G+ WW     +H   R             
Sbjct: 101 W-KGDKDQWILFTDIEGLVIEGD--GEINGQGSSWW-----EHKGSRP-----------T 160

Query: 121 AMKIISCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNV 180
           A+K  SCNN  LS +  ++S   H+ I     V I +L I APESSPNTDGI + +S NV
Sbjct: 161 ALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGIDVGASSNV 220

Query: 181 IIKNTTIGTGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYF 240
           +I++  I TGDDC++I   TSN++IS I CGPGHGISIGSLG+ G    VE++ V N  F
Sbjct: 221 VIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDGETATVENVCVQNCNF 280

Query: 241 KGTTNGARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNY 279
           +GT NGARIKTWQ G G  R +TF  +  ++V NP IIDQ Y
Sbjct: 281 RGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFY 301

[Close]

BLAST of MC08g2448 vs. ExPASy Swiss-Prot
Match: Q9SFB7 (Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2)

HSP 1 Score: 223.8 bits (569), Expect = 2.4e-57
Identity = 115/282 (40.78%), Postives = 168/282 (59.57%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGRCKFS----QIHGEIVAPTSPGE 60
           +A +KAW+AACSS T    ++ PK    ++K +   G CK S    +I+G I A  +P +
Sbjct: 87  KAFMKAWEAACSS-TGIVYIVAPKNRDYMLKAVTFSGPCKSSLIIFKIYGRIEAWENPSD 146

Query: 61  WTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLRVFGELTKSRALLQ 120
           +   ++  W+ FE V+ L V G   G+IDG G  WW +SC+ +P L   G  T       
Sbjct: 147 Y--KERRHWIVFENVNNLRVEGG--GRIDGNGHIWWPKSCKINPQLPCLGAPT------- 206

Query: 121 AMKIISCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNV 180
           A+  + CNN  +SNI   N+ Q H+     K V+  NL++T+P  SPNTDGIH+  + N+
Sbjct: 207 AVTFVECNNLRVSNIRLENAQQMHLTFQDCKNVKALNLMVTSPADSPNTDGIHVSGTQNI 266

Query: 181 IIKNTTIGTGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYF 240
           +I+++ + TGDDC+SI   + NV  + I CGPGHGISIGSLG   +   V ++ V+    
Sbjct: 267 LIQDSIVRTGDDCISIVSGSENVRATGITCGPGHGISIGSLGEDNSEAYVSNVVVNKATL 326

Query: 241 KGTTNGARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNY 279
            GTTNG RIKTWQ G G  + + FQ++  ++V NP II+Q+Y
Sbjct: 327 IGTTNGVRIKTWQGGHGMAKNIIFQDIIMKNVTNPIIINQDY 356

[Close]

BLAST of MC08g2448 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 3.9e-55
Identity = 121/281 (43.06%), Postives = 160/281 (56.94%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGRCKFS---QIHGEIVAPTSPGEW 60
           +A  KAWKAACSS T S+ +L+PK++  LV+PI   G CK     QI+G I A     ++
Sbjct: 106 KAFEKAWKAACSS-TSSAVLLVPKKN-YLVRPISFSGPCKSGLTMQIYGTIEASDDRSDY 165

Query: 61  TRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLRVFGELTKSRALLQA 120
            R D   WL F+ V  L V G   G I+G G  WW  SC+ +  L      T       A
Sbjct: 166 -RKDGRHWLVFDSVQNLRVEGG--GTINGNGKIWWQNSCKTNKALPCKDAPT-------A 225

Query: 121 MKIISCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVI 180
           +      +  + N+   N+ Q HV       V+  NL++TAPE+SPNTDGIH+  + N+ 
Sbjct: 226 LTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENSPNTDGIHVTGTQNIH 285

Query: 181 IKNTTIGTGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFK 240
           I +  IGTGDDC+SI + +  V ++ I CGPGHGISIGSLG   +   V D+ V+     
Sbjct: 286 ISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGISIGSLGYGNSEAHVSDVVVNGAKLC 345

Query: 241 GTTNGARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNY 279
           GTTNG RIKTWQ G G+   + FQN+   +V NP IIDQNY
Sbjct: 346 GTTNGVRIKTWQGGSGSASNIKFQNVEMHNVENPIIIDQNY 374

[Close]

BLAST of MC08g2448 vs. ExPASy Swiss-Prot
Match: O23147 (Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 9.6e-54
Identity = 117/284 (41.20%), Postives = 161/284 (56.69%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGRCKF---SQIHGEIVAPTSPGEW 60
           QA  KAWK ACS+    +T L+PK  T L+K    RG CK     QI G + A T   ++
Sbjct: 85  QAFKKAWKKACST-NGVTTFLVPKGKTYLLKSTRFRGPCKSLRNFQILGTLSASTKRSDY 144

Query: 61  TRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLRVFGELTKSRALLQ- 120
              D++ WL  E V+ L++ G   G I+G G  WW  SC+          + KS+   + 
Sbjct: 145 --KDKNHWLILEDVNNLSIDGGSTGIINGNGKTWWQNSCK----------IDKSKPCTKA 204

Query: 121 --AMKIISCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSH 180
             A+ + +  N ++ N+   N+ Q  + I    +VE+ N+ ITAP  SPNTDGIHI ++ 
Sbjct: 205 PTALTLYNLKNLNVKNLRVKNAQQIQISIEKCNKVEVSNVEITAPGDSPNTDGIHITNTQ 264

Query: 181 NVIIKNTTIGTGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNV 240
           N+ + N+ IGTGDDC+SI D T N+ I  + CGPGHGISIGSLG   +   V  I V   
Sbjct: 265 NIRVSNSDIGTGDDCISIEDGTQNLQIFDLTCGPGHGISIGSLGDDNSKAYVSGINVDGA 324

Query: 241 YFKGTTNGARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNY 279
            F  + NG RIKT+Q G G  + + FQN+  E+V NP IIDQ+Y
Sbjct: 325 KFSESDNGVRIKTYQGGSGTAKNIKFQNIRMENVKNPIIIDQDY 355

[Close]

BLAST of MC08g2448 vs. NCBI nr
Match: XP_022143115.1 (probable polygalacturonase At1g80170 [Momordica charantia])

HSP 1 Score: 518 bits (1335), Expect = 9.07e-183
Identity = 259/281 (92.17%), Postives = 263/281 (93.59%), Query Frame = 0


Query: 2   AILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGRCKFSQI----HGEIVAPTSPGEW 61
           AILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRG C+ + I    HGEIVAPTSPGEW
Sbjct: 47  AILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGPCRANSITFMIHGEIVAPTSPGEW 106

Query: 62  TRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLRVFGELTKSRALLQA 121
           TRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDL+    L  +     A
Sbjct: 107 TRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLKGCTSLAPT-----A 166

Query: 122 MKIISCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVI 181
           MKIISCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVI
Sbjct: 167 MKIISCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVI 226

Query: 182 IKNTTIGTGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFK 241
           IKNTTIGTGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFK
Sbjct: 227 IKNTTIGTGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFK 286

Query: 242 GTTNGARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNY 278
           GTTNGARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNY
Sbjct: 287 GTTNGARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNY 322

[Close]

BLAST of MC08g2448 vs. NCBI nr
Match: KAA0061603.1 (putative polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 398 bits (1023), Expect = 1.42e-135
Identity = 203/305 (66.56%), Postives = 231/305 (75.74%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRG----------------------- 60
           QAI KAWKAAC+SKT+SSTVLIPKR T++VKPI+L G                       
Sbjct: 46  QAISKAWKAACNSKTKSSTVLIPKRQTVMVKPILLSGPCRAKSITFMVKKNRDFPLMIKS 105

Query: 61  ---------RCKFSQIHGEIVAPTSPGEWTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRG 120
                    RCK SQI+G IVAPTSP EW  TD+S WLAF+G+SRL++TG+GIGKIDGRG
Sbjct: 106 FLEYYLSSGRCKLSQINGVIVAPTSPKEWAGTDKSLWLAFKGISRLSITGSGIGKIDGRG 165

Query: 121 TRWWSQSCRDHPDLRVFGELTKSRALLQAMKIISCNNTSLSNIHFINSSQTHVLIMGSKR 180
           T WW+QSCRDHP L           LLQA KIISCN+T+LSN+HFINSSQ HVL+M  +R
Sbjct: 166 TGWWNQSCRDHPSL----------PLLQATKIISCNDTNLSNLHFINSSQAHVLLMECRR 225

Query: 181 VEIKNLVITAPESSPNTDGIHIHSSHNVIIKNTTIGTGDDCVSIGDYTSNVNISHIRCGP 240
            EIKNLVITAPESSPNTDGIHIHSSHN+IIKNTTIGTGDDCVS GDYTS+VNISHI+CGP
Sbjct: 226 TEIKNLVITAPESSPNTDGIHIHSSHNIIIKNTTIGTGDDCVSTGDYTSHVNISHIKCGP 285

Query: 241 GHGISIGSLGRSGNFVQVEDIRVSNVYFKGTTNGARIKTWQIGRGNIRRVTFQNLYFESV 273
           GHGISIGSLG+SGNFVQVEDI VSN+YFKGTTN ARIKTWQ     I  V+ +NL+  S+
Sbjct: 286 GHGISIGSLGKSGNFVQVEDISVSNIYFKGTTNRARIKTWQQSGVRIANVSIKNLFGTSM 340

[Close]

BLAST of MC08g2448 vs. NCBI nr
Match: XP_016902034.1 (PREDICTED: probable polygalacturonase At1g80170 isoform X1 [Cucumis melo])

HSP 1 Score: 385 bits (990), Expect = 5.97e-132
Identity = 189/256 (73.83%), Postives = 216/256 (84.38%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGRCKFSQI----HGEIVAPTSPGE 60
           QAI +AWKAAC+SKT+SSTVLIPKR T++VKPI+L G C+   I    +G I+APTSP E
Sbjct: 46  QAISEAWKAACNSKTKSSTVLIPKRQTVMVKPILLSGPCRVKSITFMINGVIMAPTSPKE 105

Query: 61  WTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLRVFGELTKSRALLQ 120
           W  TD+S WLAFEG+SRL++TG+ IGKIDGRGT WW+QSCRDHP L       + R L+ 
Sbjct: 106 WAGTDKSLWLAFEGISRLSITGSSIGKIDGRGTGWWNQSCRDHPSL-------EGRTLIA 165

Query: 121 --AMKIISCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSH 180
             A KIISCN+T+LSN+HFINSSQ HVL+M  +R EIKNLVITAPESSPNTDGIHIHSSH
Sbjct: 166 PTATKIISCNDTNLSNLHFINSSQAHVLLMECRRTEIKNLVITAPESSPNTDGIHIHSSH 225

Query: 181 NVIIKNTTIGTGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNV 240
           N+IIKNTTIGTGDDCVS GDYTS VNISHI+CGPGHGISIGSLG+SGNFVQVEDI VSN+
Sbjct: 226 NIIIKNTTIGTGDDCVSTGDYTSRVNISHIKCGPGHGISIGSLGKSGNFVQVEDISVSNI 285

Query: 241 YFKGTTNGARIKTWQI 250
           YFKGTTN ARIKTWQ+
Sbjct: 286 YFKGTTNRARIKTWQV 294

[Close]

BLAST of MC08g2448 vs. NCBI nr
Match: TYJ97813.1 (putative polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 362 bits (928), Expect = 1.90e-121
Identity = 182/269 (67.66%), Postives = 210/269 (78.07%), Query Frame = 0


Query: 7   WKAACSSKTESSTVLIPKRHTLLVKPIVLRGRCKFSQIHGEIVAPTSPGEWTRTDQSQWL 66
           W+  C      ST    +R  LL K   + GRCK SQI+G IVAPTSP EW  TD+S WL
Sbjct: 60  WEQGCEEFCSDSTGKFWERVALL-KSYSIVGRCKLSQINGVIVAPTSPKEWAGTDKSLWL 119

Query: 67  AFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLRVFGELTKSRALLQ--AMKIISCN 126
           AF+G+SRL++TG+GIGKIDGRGT WW+QSCRDHP L       + R L+   A KIISCN
Sbjct: 120 AFKGISRLSITGSGIGKIDGRGTGWWNQSCRDHPSL-------EGRTLIAPTATKIISCN 179

Query: 127 NTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNTTIG 186
           +T+LSN+HFINSSQ HVL+M  +R EIKNLVITAPESSPNTDGIHIHSSHN+IIKNTTIG
Sbjct: 180 DTNLSNLHFINSSQAHVLLMECRRTEIKNLVITAPESSPNTDGIHIHSSHNIIIKNTTIG 239

Query: 187 TGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTTNGAR 246
           TGDDCVS GDYTS+VNISHI+CGPGHGISIGSLG+SGNFVQVEDI VSN+YFKGTTN AR
Sbjct: 240 TGDDCVSTGDYTSHVNISHIKCGPGHGISIGSLGKSGNFVQVEDISVSNIYFKGTTNRAR 299

Query: 247 IKTWQIGRGNIRRVTFQNLYFESVNNPFI 273
           IKTWQ     I  V+ +NL+  S++N  I
Sbjct: 300 IKTWQQSGVRIANVSIKNLFGTSMSNVAI 320

[Close]

BLAST of MC08g2448 vs. NCBI nr
Match: VVA10736.1 (PREDICTED: polygalacturonase [Prunus dulcis])

HSP 1 Score: 356 bits (914), Expect = 2.70e-119
Identity = 173/282 (61.35%), Postives = 215/282 (76.24%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGRCKFSQIH----GEIVAPTSPGE 60
           QA LKAW AAC+S+TE+ T+++P++  LL  PIV  G C+   I+    G ++AP SPG 
Sbjct: 15  QAFLKAWNAACTSETENPTLIVPRKRFLL-NPIVFSGPCRAENINFLIFGRLLAPDSPGA 74

Query: 61  WTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLRVFGELTKSRALLQ 120
           W   D SQWLAF GVS LN+ G   G+I+GRG  WW QSCRDHP L     L  +     
Sbjct: 75  WKELDPSQWLAFNGVSGLNIAGP--GRINGRGKAWWDQSCRDHPRLVGCSTLAPT----- 134

Query: 121 AMKIISCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNV 180
           A+K++SC N+S+S IHF+NSSQTHVLI  S  + I+N++I APE SPNTDGIHI +SHNV
Sbjct: 135 AVKLVSCKNSSISEIHFLNSSQTHVLIRDSDGINIENVLIEAPERSPNTDGIHISASHNV 194

Query: 181 IIKNTTIGTGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYF 240
           +I N  IGTGDDCVSIGD+TSN+ IS+++CGPGHGISIGSLGRSGNFVQVE+I VS VY 
Sbjct: 195 VITNAIIGTGDDCVSIGDHTSNIVISYVKCGPGHGISIGSLGRSGNFVQVENIHVSKVYL 254

Query: 241 KGTTNGARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNY 278
           +GTTNGARIKTWQ+GRG +R+VTF++++F SV NP IIDQNY
Sbjct: 255 QGTTNGARIKTWQVGRGYVRQVTFEHIFFGSVKNPIIIDQNY 288

[Close]

BLAST of MC08g2448 vs. ExPASy TrEMBL
Match: A0A6J1CMV4 (probable polygalacturonase At1g80170 OS=Momordica charantia OX=3673 GN=LOC111013063 PE=3 SV=1)

HSP 1 Score: 518 bits (1335), Expect = 4.39e-183
Identity = 259/281 (92.17%), Postives = 263/281 (93.59%), Query Frame = 0


Query: 2   AILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGRCKFSQI----HGEIVAPTSPGEW 61
           AILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRG C+ + I    HGEIVAPTSPGEW
Sbjct: 47  AILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGPCRANSITFMIHGEIVAPTSPGEW 106

Query: 62  TRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLRVFGELTKSRALLQA 121
           TRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDL+    L  +     A
Sbjct: 107 TRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLKGCTSLAPT-----A 166

Query: 122 MKIISCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVI 181
           MKIISCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVI
Sbjct: 167 MKIISCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVI 226

Query: 182 IKNTTIGTGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFK 241
           IKNTTIGTGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFK
Sbjct: 227 IKNTTIGTGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFK 286

Query: 242 GTTNGARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNY 278
           GTTNGARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNY
Sbjct: 287 GTTNGARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNY 322

[Close]

BLAST of MC08g2448 vs. ExPASy TrEMBL
Match: A0A5A7V0D8 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G001680 PE=3 SV=1)

HSP 1 Score: 398 bits (1023), Expect = 6.86e-136
Identity = 203/305 (66.56%), Postives = 231/305 (75.74%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRG----------------------- 60
           QAI KAWKAAC+SKT+SSTVLIPKR T++VKPI+L G                       
Sbjct: 46  QAISKAWKAACNSKTKSSTVLIPKRQTVMVKPILLSGPCRAKSITFMVKKNRDFPLMIKS 105

Query: 61  ---------RCKFSQIHGEIVAPTSPGEWTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRG 120
                    RCK SQI+G IVAPTSP EW  TD+S WLAF+G+SRL++TG+GIGKIDGRG
Sbjct: 106 FLEYYLSSGRCKLSQINGVIVAPTSPKEWAGTDKSLWLAFKGISRLSITGSGIGKIDGRG 165

Query: 121 TRWWSQSCRDHPDLRVFGELTKSRALLQAMKIISCNNTSLSNIHFINSSQTHVLIMGSKR 180
           T WW+QSCRDHP L           LLQA KIISCN+T+LSN+HFINSSQ HVL+M  +R
Sbjct: 166 TGWWNQSCRDHPSL----------PLLQATKIISCNDTNLSNLHFINSSQAHVLLMECRR 225

Query: 181 VEIKNLVITAPESSPNTDGIHIHSSHNVIIKNTTIGTGDDCVSIGDYTSNVNISHIRCGP 240
            EIKNLVITAPESSPNTDGIHIHSSHN+IIKNTTIGTGDDCVS GDYTS+VNISHI+CGP
Sbjct: 226 TEIKNLVITAPESSPNTDGIHIHSSHNIIIKNTTIGTGDDCVSTGDYTSHVNISHIKCGP 285

Query: 241 GHGISIGSLGRSGNFVQVEDIRVSNVYFKGTTNGARIKTWQIGRGNIRRVTFQNLYFESV 273
           GHGISIGSLG+SGNFVQVEDI VSN+YFKGTTN ARIKTWQ     I  V+ +NL+  S+
Sbjct: 286 GHGISIGSLGKSGNFVQVEDISVSNIYFKGTTNRARIKTWQQSGVRIANVSIKNLFGTSM 340

[Close]

BLAST of MC08g2448 vs. ExPASy TrEMBL
Match: A0A1S4E1D2 (probable polygalacturonase At1g80170 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497273 PE=3 SV=1)

HSP 1 Score: 385 bits (990), Expect = 2.89e-132
Identity = 189/256 (73.83%), Postives = 216/256 (84.38%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGRCKFSQI----HGEIVAPTSPGE 60
           QAI +AWKAAC+SKT+SSTVLIPKR T++VKPI+L G C+   I    +G I+APTSP E
Sbjct: 46  QAISEAWKAACNSKTKSSTVLIPKRQTVMVKPILLSGPCRVKSITFMINGVIMAPTSPKE 105

Query: 61  WTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLRVFGELTKSRALLQ 120
           W  TD+S WLAFEG+SRL++TG+ IGKIDGRGT WW+QSCRDHP L       + R L+ 
Sbjct: 106 WAGTDKSLWLAFEGISRLSITGSSIGKIDGRGTGWWNQSCRDHPSL-------EGRTLIA 165

Query: 121 --AMKIISCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSH 180
             A KIISCN+T+LSN+HFINSSQ HVL+M  +R EIKNLVITAPESSPNTDGIHIHSSH
Sbjct: 166 PTATKIISCNDTNLSNLHFINSSQAHVLLMECRRTEIKNLVITAPESSPNTDGIHIHSSH 225

Query: 181 NVIIKNTTIGTGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNV 240
           N+IIKNTTIGTGDDCVS GDYTS VNISHI+CGPGHGISIGSLG+SGNFVQVEDI VSN+
Sbjct: 226 NIIIKNTTIGTGDDCVSTGDYTSRVNISHIKCGPGHGISIGSLGKSGNFVQVEDISVSNI 285

Query: 241 YFKGTTNGARIKTWQI 250
           YFKGTTN ARIKTWQ+
Sbjct: 286 YFKGTTNRARIKTWQV 294

[Close]

BLAST of MC08g2448 vs. ExPASy TrEMBL
Match: A0A5D3BEX0 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G00230 PE=3 SV=1)

HSP 1 Score: 362 bits (928), Expect = 9.21e-122
Identity = 182/269 (67.66%), Postives = 210/269 (78.07%), Query Frame = 0


Query: 7   WKAACSSKTESSTVLIPKRHTLLVKPIVLRGRCKFSQIHGEIVAPTSPGEWTRTDQSQWL 66
           W+  C      ST    +R  LL K   + GRCK SQI+G IVAPTSP EW  TD+S WL
Sbjct: 60  WEQGCEEFCSDSTGKFWERVALL-KSYSIVGRCKLSQINGVIVAPTSPKEWAGTDKSLWL 119

Query: 67  AFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLRVFGELTKSRALLQ--AMKIISCN 126
           AF+G+SRL++TG+GIGKIDGRGT WW+QSCRDHP L       + R L+   A KIISCN
Sbjct: 120 AFKGISRLSITGSGIGKIDGRGTGWWNQSCRDHPSL-------EGRTLIAPTATKIISCN 179

Query: 127 NTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNTTIG 186
           +T+LSN+HFINSSQ HVL+M  +R EIKNLVITAPESSPNTDGIHIHSSHN+IIKNTTIG
Sbjct: 180 DTNLSNLHFINSSQAHVLLMECRRTEIKNLVITAPESSPNTDGIHIHSSHNIIIKNTTIG 239

Query: 187 TGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTTNGAR 246
           TGDDCVS GDYTS+VNISHI+CGPGHGISIGSLG+SGNFVQVEDI VSN+YFKGTTN AR
Sbjct: 240 TGDDCVSTGDYTSHVNISHIKCGPGHGISIGSLGKSGNFVQVEDISVSNIYFKGTTNRAR 299

Query: 247 IKTWQIGRGNIRRVTFQNLYFESVNNPFI 273
           IKTWQ     I  V+ +NL+  S++N  I
Sbjct: 300 IKTWQQSGVRIANVSIKNLFGTSMSNVAI 320

[Close]

BLAST of MC08g2448 vs. ExPASy TrEMBL
Match: A0A5E4E632 (PREDICTED: polygalacturonase OS=Prunus dulcis OX=3755 GN=ALMOND_2B007689 PE=3 SV=1)

HSP 1 Score: 356 bits (914), Expect = 1.31e-119
Identity = 173/282 (61.35%), Postives = 215/282 (76.24%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGRCKFSQIH----GEIVAPTSPGE 60
           QA LKAW AAC+S+TE+ T+++P++  LL  PIV  G C+   I+    G ++AP SPG 
Sbjct: 15  QAFLKAWNAACTSETENPTLIVPRKRFLL-NPIVFSGPCRAENINFLIFGRLLAPDSPGA 74

Query: 61  WTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLRVFGELTKSRALLQ 120
           W   D SQWLAF GVS LN+ G   G+I+GRG  WW QSCRDHP L     L  +     
Sbjct: 75  WKELDPSQWLAFNGVSGLNIAGP--GRINGRGKAWWDQSCRDHPRLVGCSTLAPT----- 134

Query: 121 AMKIISCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNV 180
           A+K++SC N+S+S IHF+NSSQTHVLI  S  + I+N++I APE SPNTDGIHI +SHNV
Sbjct: 135 AVKLVSCKNSSISEIHFLNSSQTHVLIRDSDGINIENVLIEAPERSPNTDGIHISASHNV 194

Query: 181 IIKNTTIGTGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYF 240
           +I N  IGTGDDCVSIGD+TSN+ IS+++CGPGHGISIGSLGRSGNFVQVE+I VS VY 
Sbjct: 195 VITNAIIGTGDDCVSIGDHTSNIVISYVKCGPGHGISIGSLGRSGNFVQVENIHVSKVYL 254

Query: 241 KGTTNGARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNY 278
           +GTTNGARIKTWQ+GRG +R+VTF++++F SV NP IIDQNY
Sbjct: 255 QGTTNGARIKTWQVGRGYVRQVTFEHIFFGSVKNPIIIDQNY 288

[Close]

BLAST of MC08g2448 vs. TAIR 10
Match: AT1G80170.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 240.4 bits (612), Expect = 1.8e-63
Identity = 121/281 (43.06%), Postives = 170/281 (60.50%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGRCKFS---QIHGEIVAPTSPGEW 60
           +A   AWK ACSSK + + +L+P+ +T L++PI L G CK     QI G I+AP  P  W
Sbjct: 70  KAFADAWKTACSSKVK-TRILVPENYTCLLRPIDLSGPCKARLTLQISGTIIAPNDPDVW 129

Query: 61  TRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLRVFGELTKSRALLQA 120
              ++ +WL F G+SRL V G   G ++G G  WW +SC+ +            R    A
Sbjct: 130 EGLNRRKWLYFHGLSRLTVEGG--GTVNGMGQEWWERSCKHN-------HSNPCRGAPTA 189

Query: 121 MKIISCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVI 180
           +    C N  + N++ I+S Q H+ +   +RV I  L + AP +SPNTDGIHI  S  ++
Sbjct: 190 LTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDGIHISVSRGIV 249

Query: 181 IKNTTIGTGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFK 240
           I NTT+ TGDDC+SI   ++ ++IS+I CGPGHGISIGSLG+S ++ +V DI V      
Sbjct: 250 IDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVRDITVDTAIIS 309

Query: 241 GTTNGARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNY 279
            T NG RIKTWQ G G + ++ F+N+   +V+NP IIDQ Y
Sbjct: 310 DTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYY 340

[Close]

BLAST of MC08g2448 vs. TAIR 10
Match: AT1G80140.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 232.6 bits (592), Expect = 3.7e-61
Identity = 123/266 (46.24%), Postives = 162/266 (60.90%), Query Frame = 0


Query: 19  TVLIPKRHTLLVKPIVLRGRC----KFSQIHGEIVAPTSPGEWTRTDQS--QWLAFEGVS 78
           +V +P     L+  +   G C        I GE++A + P +W   +     WL F+ V 
Sbjct: 3   SVNVPAGKVFLLNSLHFTGPCIPKPLLFIIDGEMIAQSDPQKWGNGENGVIPWLIFDQVD 62

Query: 79  RLNVTGTGIGKIDGRGTRWWSQSCRDHPDLRVFGELTKSRALLQAMKIISCNNTSLSNIH 138
            L + G G+  +DG+G  WW   CRDHP                 M   +C N +L ++ 
Sbjct: 63  GLAIVGRGL--LDGQGKSWWDIHCRDHPG--------------PMMTFSNCGNVTLKSLR 122

Query: 139 FINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNTTIGTGDDCVSI 198
           F NS+QTHVL+MGS+ V I ++ IT+PE+SPNTDGIHI SS  V I ++ I TGDDCVSI
Sbjct: 123 FRNSAQTHVLVMGSQNVYIDDIKITSPEASPNTDGIHITSSTAVSINHSDIATGDDCVSI 182

Query: 199 GDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTTNGARIKTWQIGR 258
           GD  +N+N++ + CGPGHG+SIGSLGR G  V VE+IRVS+V F GTTNGARIKTW  G 
Sbjct: 183 GDQVNNLNVTFMNCGPGHGVSIGSLGRGGTEVTVENIRVSHVNFTGTTNGARIKTWPGGT 242

Query: 259 GNIRRVTFQNLYFESVNNPFIIDQNY 279
           G +R + F ++ F SV NP IIDQ Y
Sbjct: 243 GYVRGIEFFDIRFSSVQNPIIIDQFY 252

[Close]

BLAST of MC08g2448 vs. TAIR 10
Match: AT3G15720.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 225.7 bits (574), Expect = 4.6e-59
Identity = 125/282 (44.33%), Postives = 169/282 (59.93%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGRCK----FSQIHGEIVAPTSPGE 60
           QA LKAW+A CS  T     ++P   T +++P+  +G CK    F Q+ G++VAP S G 
Sbjct: 41  QAFLKAWEAVCSG-TGDGQFVVPAGMTFMLQPLKFQGSCKSTPVFVQMLGKLVAP-SKGN 100

Query: 61  WTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLRVFGELTKSRALLQ 120
           W + D+ QW+ F  +  L + G   G+I+G+G+ WW     +H   R             
Sbjct: 101 W-KGDKDQWILFTDIEGLVIEGD--GEINGQGSSWW-----EHKGSRP-----------T 160

Query: 121 AMKIISCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNV 180
           A+K  SCNN  LS +  ++S   H+ I     V I +L I APESSPNTDGI + +S NV
Sbjct: 161 ALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGIDVGASSNV 220

Query: 181 IIKNTTIGTGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYF 240
           +I++  I TGDDC++I   TSN++IS I CGPGHGISIGSLG+ G    VE++ V N  F
Sbjct: 221 VIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDGETATVENVCVQNCNF 280

Query: 241 KGTTNGARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNY 279
           +GT NGARIKTWQ G G  R +TF  +  ++V NP IIDQ Y
Sbjct: 281 RGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFY 301

[Close]

BLAST of MC08g2448 vs. TAIR 10
Match: AT3G07970.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 223.8 bits (569), Expect = 1.7e-58
Identity = 115/282 (40.78%), Postives = 168/282 (59.57%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGRCKFS----QIHGEIVAPTSPGE 60
           +A +KAW+AACSS T    ++ PK    ++K +   G CK S    +I+G I A  +P +
Sbjct: 87  KAFMKAWEAACSS-TGIVYIVAPKNRDYMLKAVTFSGPCKSSLIIFKIYGRIEAWENPSD 146

Query: 61  WTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLRVFGELTKSRALLQ 120
           +   ++  W+ FE V+ L V G   G+IDG G  WW +SC+ +P L   G  T       
Sbjct: 147 Y--KERRHWIVFENVNNLRVEGG--GRIDGNGHIWWPKSCKINPQLPCLGAPT------- 206

Query: 121 AMKIISCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNV 180
           A+  + CNN  +SNI   N+ Q H+     K V+  NL++T+P  SPNTDGIH+  + N+
Sbjct: 207 AVTFVECNNLRVSNIRLENAQQMHLTFQDCKNVKALNLMVTSPADSPNTDGIHVSGTQNI 266

Query: 181 IIKNTTIGTGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYF 240
           +I+++ + TGDDC+SI   + NV  + I CGPGHGISIGSLG   +   V ++ V+    
Sbjct: 267 LIQDSIVRTGDDCISIVSGSENVRATGITCGPGHGISIGSLGEDNSEAYVSNVVVNKATL 326

Query: 241 KGTTNGARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNY 279
            GTTNG RIKTWQ G G  + + FQ++  ++V NP II+Q+Y
Sbjct: 327 IGTTNGVRIKTWQGGHGMAKNIIFQDIIMKNVTNPIIINQDY 356

[Close]

BLAST of MC08g2448 vs. TAIR 10
Match: AT3G15720.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 221.5 bits (563), Expect = 8.6e-58
Identity = 123/282 (43.62%), Postives = 165/282 (58.51%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGRCK----FSQIHGEIVAPTSPGE 60
           QA LKAW+A CS  T     ++P   T +++P+  +G CK    F Q+ G++VAP S G 
Sbjct: 40  QAFLKAWEAVCSG-TGDGQFVVPAGMTFMLQPLKFQGSCKSTPVFVQMLGKLVAP-SKGN 99

Query: 61  WTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLRVFGELTKSRALLQ 120
           W + D+ QW+ F  +  L + G   G+I+G+G+ WW       P  R             
Sbjct: 100 W-KGDKDQWILFTDIEGLVIEGD--GEINGQGSSWWEHK-GSRPTFR------------- 159

Query: 121 AMKIISCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNV 180
                SCNN  LS +  ++S   H+ I     V I +L I APESSPNTDGI + +S NV
Sbjct: 160 -----SCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGIDVGASSNV 219

Query: 181 IIKNTTIGTGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYF 240
           +I++  I TGDDC++I   TSN++IS I CGPGHGISIGSLG+ G    VE++ V N  F
Sbjct: 220 VIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDGETATVENVCVQNCNF 279

Query: 241 KGTTNGARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNY 279
           +GT NGARIKTWQ G G  R +TF  +  ++V NP IIDQ Y
Sbjct: 280 RGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFY 297

The following BLAST results are available for this feature:

BLAST of MC08g2448 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94AJ5	2.5e-62	43.06	Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017...	[more]
Q9LW07	6.4e-58	44.33	Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572...	[more]
Q9SFB7	2.4e-57	40.78	Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2	[more]
P35336	3.9e-55	43.06	Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1	[more]
O23147	9.6e-54	41.20	Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1	[more]

BLAST of MC08g2448 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143115.1	9.07e-183	92.17	probable polygalacturonase At1g80170 [Momordica charantia]	[more]
KAA0061603.1	1.42e-135	66.56	putative polygalacturonase [Cucumis melo var. makuwa]	[more]
XP_016902034.1	5.97e-132	73.83	PREDICTED: probable polygalacturonase At1g80170 isoform X1 [Cucumis melo]	[more]
TYJ97813.1	1.90e-121	67.66	putative polygalacturonase [Cucumis melo var. makuwa]	[more]
VVA10736.1	2.70e-119	61.35	PREDICTED: polygalacturonase [Prunus dulcis]	[more]

BLAST of MC08g2448 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CMV4	4.39e-183	92.17	probable polygalacturonase At1g80170 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A5A7V0D8	6.86e-136	66.56	Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff...	[more]
A0A1S4E1D2	2.89e-132	73.83	probable polygalacturonase At1g80170 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A5D3BEX0	9.21e-122	67.66	Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A5E4E632	1.31e-119	61.35	PREDICTED: polygalacturonase OS=Prunus dulcis OX=3755 GN=ALMOND_2B007689 PE=3 SV...	[more]

BLAST of MC08g2448 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G80170.1	1.8e-63	43.06	Pectin lyase-like superfamily protein	[more]
AT1G80140.1	3.7e-61	46.24	Pectin lyase-like superfamily protein	[more]
AT3G15720.1	4.6e-59	44.33	Pectin lyase-like superfamily protein	[more]
AT3G07970.1	1.7e-58	40.78	Pectin lyase-like superfamily protein	[more]
AT3G15720.2	8.6e-58	43.62	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 226..247 e-value: 120.0 score: 11.5 coord: 146..172 e-value: 310.0 score: 10.1 coord: 173..194 e-value: 840.0 score: 7.8 coord: 196..216 e-value: 5000.0 score: 2.3
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 1..278 e-value: 4.2E-82 score: 278.3
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 14..278 e-value: 3.7E-64 score: 216.8
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 203..216
None	No IPR available	PANTHER	PTHR31375:SF229	POLYGALACTURONASE-LIKE	coord: 1..278
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 1..278
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 1..278

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g2448.1	MC08g2448.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity