MC08g2416 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g2416 vs. ExPASy Swiss-Prot
Match: Q9LEW0 (Ubiquitin C-terminal hydrolase 22 OS=Arabidopsis thaliana OX=3702 GN=UBP22 PE=1 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 8.1e-146
Identity = 293/591 (49.58%), Postives = 372/591 (62.94%), Query Frame = 0

Query: 30  PPSCPHLADFRSR---NGSKSFRLLQDCLRVKPPGGRAAIRRDPDEVPRCSFCGDSALPR 89
           P  C HL+D++ R   +G KSF  L  C        R  I+    ++PRCS+C      R
Sbjct: 11  PDPCNHLSDYKLRYGTDGYKSFNNLFRCFN----DARIKIKLQGIDIPRCSYC-SVYQKR 70

Query: 90  LYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRAELFCCACRDQVYDRDFDAA 149
           LY C+ C ++SC      SH   H + +  GH+IA+DV+R+EL+CCAC DQVYD +FD  
Sbjct: 71  LYICLICRSISC-----SSHILLHTQ-LNKGHDIAIDVERSELYCCACIDQVYDSEFDEV 130

Query: 150 VVLAQTTASTLGGNVVSSSGAPPQPLLPPENLRKRRRVDYQPWTPDLRERVLMGAN--SS 209
           VV  Q      G  +   SGA     +      K+RR+D Q         +++G+N   S
Sbjct: 131 VVSKQ----LFGLGMSVKSGAD----VVAVRSNKKRRLDSQ---------LIIGSNFLVS 190

Query: 210 PLDGANSNMGSESEMPSGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQR 269
           P D        +   P GLRGLNNLG+TCFMN+VLQAL+H PPLRNF+LS +HNR  C R
Sbjct: 191 PRD-----RREKWTFPLGLRGLNNLGSTCFMNAVLQALVHAPPLRNFWLSGQHNRDLCPR 250

Query: 270 NVNVGNKNSNSNDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSWWQHAANLA 329
                                +CL CDLD +FSA+FSGDRTPYSPA  L+SWWQH+ NLA
Sbjct: 251 RT----------------MGLLCLPCDLDVIFSAMFSGDRTPYSPAHLLYSWWQHSTNLA 310

Query: 330 SYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDC-CIAHRVFSGILRSDVMCMACGF 389
           +YEQQD+HEFFIS+L+ IHE  ++ + K   Q N +C CI H+ FSG+LRSDV C  CG 
Sbjct: 311 TYEQQDSHEFFISLLDRIHE--NEGKSKCLYQDNEECQCITHKAFSGLLRSDVTCTTCGS 370

Query: 390 TSTTYDPCVDISLDLEPNLGGSAKMAATRSNLSCNGEADCVNSSQNHRISSLVGCLDQFT 449
           TSTTYDP +DISL L+   G S   A  R N    G       S N  + +L GCLD FT
Sbjct: 371 TSTTYDPFIDISLTLDSMNGFSP--ADCRKNRYSGG------PSVNAIMPTLSGCLDFFT 430

Query: 450 RPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQF 509
           R EKLG DQK  CQ C  ++ES KQMSIR+LPL+ C H+KRFEHS  RK SRK+D YLQ+
Sbjct: 431 RSEKLGPDQKLNCQSCGEKRESSKQMSIRRLPLLLCLHVKRFEHSLTRKTSRKIDSYLQY 490

Query: 510 PFSLDMAPYLSSSILRSRFGNRIFPFDGD-EQDACNES--SSEFELFAVITHKGKLDAGH 569
           PF L+M+PYLSSSI+  RFGNRIF FDG+ E D+ + S  S+EFE+FAV+THKG L++GH
Sbjct: 491 PFRLNMSPYLSSSIIGKRFGNRIFAFDGEGEYDSSSSSSPSAEFEIFAVVTHKGMLESGH 542

Query: 570 YVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQS 612
           YVTYLRL   WY+CDDAWI +V E +VR  + YM+FY Q+ +  KA ++ S
Sbjct: 551 YVTYLRLKGLWYRCDDAWINEVEEEVVRGCECYMLFYAQETVIQKAHKELS 542

BLAST of MC08g2416 vs. ExPASy Swiss-Prot
Match: Q5DU02 (Ubiquitin carboxyl-terminal hydrolase 22 OS=Mus musculus OX=10090 GN=Usp22 PE=2 SV=2)

HSP 1 Score: 327.8 bits (839), Expect = 2.6e-88
Identity = 209/579 (36.10%), Postives = 292/579 (50.43%), Query Frame = 0

Query: 30  PPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIRRDPDEVPRCSFCGDSALPRLYA 89
           PP C HL  F+  N  ++ R +  C       G A  R+   +   C  CG   L RL++
Sbjct: 20  PPGCSHLGSFKVDNWKQNLRAIYQCF---VWSGTAEARKRKAKSCVCHVCG-IHLNRLHS 79

Query: 90  CVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRAELFCCACRDQVYDRDFDAAVVL 149
           C+ C    C    +  H   HA+S    H +A+D+    ++C  C+D +YD+D +     
Sbjct: 80  CLYCVFFGCF---TKKHIHDHAKS--KRHNLAIDLMYGGIYCFLCQDYIYDKDIEIIAKE 139

Query: 150 AQTTASTLGGNVVSSSGAPPQPLLPPENLRKRRRVDYQPWTPDLRERVLMGANSSPLDGA 209
            Q  A  + G  V                       +  W P  RE  L+  N       
Sbjct: 140 EQRKAWKMQG--VGEK--------------------FSTWEPTKRELELLKHNP------ 199

Query: 210 NSNMGSESEMPSGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQRNVNVG 269
                  S    GLRGL NLGNTCFMN ++QAL HTP LR+FFLSDRH    C+      
Sbjct: 200 -KRRKITSNCTIGLRGLINLGNTCFMNCIVQALTHTPLLRDFFLSDRHR---CE------ 259

Query: 270 NKNSNSNDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSWWQHAANLASYEQQ 329
                       ++   CL C++ ++F   +SG R+P+ P K L   W HA +LA YEQQ
Sbjct: 260 -----------MQSPSSCLVCEMSSLFQEFYSGHRSPHIPYKLLHLVWTHARHLAGYEQQ 319

Query: 330 DAHEFFISILEGIHEKVDKDRRKPYSQGNGDC-CIAHRVFSGILRSDVMCMACGFTSTTY 389
           DAHEF I+ L+ +H     D     +     C CI  ++F+G L+SDV C  C   STT 
Sbjct: 320 DAHEFLIAALDVLHRHCKGDDNGKKANNPNHCNCIIDQIFTGGLQSDVTCQVCHGVSTTI 379

Query: 390 DPCVDISLDLEPNLGGSAKMAATRSNLSCNGEADCVN-SSQNHRISSLVGCLDQFTRPEK 449
           DP  DISLDL    G S         LS   E   VN  S     ++L  CL +FTRPE 
Sbjct: 380 DPFWDISLDLP---GSSTPFWP----LSPGSEGSVVNGESHASGTTTLTDCLRRFTRPEH 439

Query: 450 LGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSL 509
           LGS  K  C  C   QES KQ++++KLP+V+CFH+KRFEHS+  K+ RK+  Y+ FP  L
Sbjct: 440 LGSSAKIKCSGCHSYQESTKQLTMKKLPIVACFHLKRFEHSA--KLRRKITTYVSFPLEL 499

Query: 510 DMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHKGKLDAGHYVTYLRL 569
           DM P+++SS      G    P D    D      +++ LFAV+ H+G L++GHY +++R 
Sbjct: 500 DMTPFMASSKESRMNGQYQQPLDSLNND------NKYSLFAVVNHQGTLESGHYTSFIRQ 525

Query: 570 -SNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYK 606
             +QW+KCDDA IT+ +   V  ++GY++FY ++ L Y+
Sbjct: 560 HKDQWFKCDDAIITKASIKDVLDSEGYLLFYHKQFLEYE 525

BLAST of MC08g2416 vs. ExPASy Swiss-Prot
Match: A6H8I0 (Ubiquitin carboxyl-terminal hydrolase 22 OS=Danio rerio OX=7955 GN=usp22 PE=2 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 3.5e-88
Identity = 209/578 (36.16%), Postives = 293/578 (50.69%), Query Frame = 0

Query: 30  PPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIRRDPDEVPRCSFCGDSALPRLYA 89
           P  C H+  F+  N  ++ R++  C       G A  R+   +   C  CG + L RL++
Sbjct: 3   PAGCSHVNGFKVDNWKQNLRVIYQCF---VWSGSAETRKRKAKSCICHMCG-AHLNRLHS 62

Query: 90  CVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRAELFCCACRDQVYDRDFDAAVVL 149
           C+ C    C    S  H   HA++    H +A+D+    ++C  C+D +YD+D +     
Sbjct: 63  CLHCVFFGCF---SKKHIHEHAKN--KRHNLAIDLLYGGIYCFVCQDYIYDKDMEQIAKE 122

Query: 150 AQTTASTLGGNVVSSSGAPPQPLLPPENLRKRRRVDYQPWTPDLRERVLMGANSSPLDGA 209
            Q  A  L G                          Y  W P  RE  L+  N       
Sbjct: 123 EQRKAWKLQG----------------------IGEKYSMWEPTKRELELLRHNP------ 182

Query: 210 NSNMGSESEMPSGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQRNVNVG 269
                  +    GLRGL NLGNTCFMN ++QAL HTP LR+FFLSDRH    C+      
Sbjct: 183 -KRRKITANCTIGLRGLINLGNTCFMNCIVQALTHTPLLRDFFLSDRHK---CE------ 242

Query: 270 NKNSNSNDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSWWQHAANLASYEQQ 329
              SNS           CL C++  +F   +SG R+P+ P + L   W HA +LA YEQQ
Sbjct: 243 -MQSNS-----------CLVCEMSQLFQEFYSGHRSPHIPFRLLHLVWTHARHLAGYEQQ 302

Query: 330 DAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHRVFSGILRSDVMCMACGFTSTTYD 389
           DAHEF I+ L+ +H     D  K  +  N   CI  ++F+G L+SDV C  C   STT D
Sbjct: 303 DAHEFLIAALDVLHRHCKDDNGKKANNPNHCNCIIDQIFTGGLQSDVTCQVCHGVSTTID 362

Query: 390 PCVDISLDLEPNLGGSAKMAATRSNLSCNGEADCVN-SSQNHRISSLVGCLDQFTRPEKL 449
           P  DISLDL    G S         LS   +   VN  S     ++L  CL +FTRPE L
Sbjct: 363 PFWDISLDLP---GSSTPFWP----LSPGSDGSVVNGDSHPSGATTLTDCLRRFTRPEHL 422

Query: 450 GSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLD 509
           GS  K  C  C   QES KQ+++++LP+V+CFH+KRFEHS+  K+ RK+  Y+ FP  LD
Sbjct: 423 GSSAKIKCSGCHSYQESTKQLTMKRLPIVACFHLKRFEHSA--KLRRKITTYVSFPLELD 482

Query: 510 MAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHKGKLDAGHYVTYLRL- 569
           M P+++SS      G    P D    D      +++ LFAV+ H+G L++GHY T++R  
Sbjct: 483 MTPFMASSKESRMNGQYQQPVDSLNND------NKYSLFAVVNHQGTLESGHYTTFIRQH 506

Query: 570 SNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYK 606
            +QW+KCDDA IT+ +   V  ++GY++FY ++ L Y+
Sbjct: 543 KDQWFKCDDAIITKASIKDVLDSEGYLLFYHKQFLEYE 506

BLAST of MC08g2416 vs. ExPASy Swiss-Prot
Match: Q9UPT9 (Ubiquitin carboxyl-terminal hydrolase 22 OS=Homo sapiens OX=9606 GN=USP22 PE=1 SV=2)

HSP 1 Score: 327.4 bits (838), Expect = 3.5e-88
Identity = 209/581 (35.97%), Postives = 293/581 (50.43%), Query Frame = 0

Query: 28  IFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIRRDPDEVPRCSFCGDSALPRL 87
           + PP C HL  F+  N  ++ R +  C       G A  R+   +   C  CG   L RL
Sbjct: 18  VAPPGCSHLGSFKVDNWKQNLRAIYQCF---VWSGTAEARKRKAKSCICHVCG-VHLNRL 77

Query: 88  YACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRAELFCCACRDQVYDRDFDAAV 147
           ++C+ C    C     H H  A A+     H +A+D+    ++C  C+D +YD+D +   
Sbjct: 78  HSCLYCVFFGCFT-KKHIHEHAKAKR----HNLAIDLMYGGIYCFLCQDYIYDKDMEIIA 137

Query: 148 VLAQTTASTLGGNVVSSSGAPPQPLLPPENLRKRRRVDYQPWTPDLRERVLMGANSSPLD 207
              Q  A  + G  V                       +  W P  RE  L+  N     
Sbjct: 138 KEEQRKAWKMQG--VGEK--------------------FSTWEPTKRELELLKHNP---- 197

Query: 208 GANSNMGSESEMPSGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQRNVN 267
                    S    GLRGL NLGNTCFMN ++QAL HTP LR+FFLSDRH    C+    
Sbjct: 198 ---KRRKITSNCTIGLRGLINLGNTCFMNCIVQALTHTPLLRDFFLSDRHR---CE---- 257

Query: 268 VGNKNSNSNDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSWWQHAANLASYE 327
                         ++   CL C++ ++F   +SG R+P+ P K L   W HA +LA YE
Sbjct: 258 -------------MQSPSSCLVCEMSSLFQEFYSGHRSPHIPYKLLHLVWTHARHLAGYE 317

Query: 328 QQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDC-CIAHRVFSGILRSDVMCMACGFTST 387
           QQDAHEF I+ L+ +H     D     +     C CI  ++F+G L+SDV C  C   ST
Sbjct: 318 QQDAHEFLIAALDVLHRHCKGDDNGKKANNPNHCNCIIDQIFTGGLQSDVTCQVCHGVST 377

Query: 388 TYDPCVDISLDLEPNLGGSAKMAATRSNLSCNGEADCVN-SSQNHRISSLVGCLDQFTRP 447
           T DP  DISLDL    G S         LS   E + VN  S     ++L  CL +FTRP
Sbjct: 378 TIDPFWDISLDLP---GSSTPFWP----LSPGSEGNVVNGESHVSGTTTLTDCLRRFTRP 437

Query: 448 EKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPF 507
           E LGS  K  C  C   QES KQ++++KLP+V+CFH+KRFEHS+  K+ RK+  Y+ FP 
Sbjct: 438 EHLGSSAKIKCSGCHSYQESTKQLTMKKLPIVACFHLKRFEHSA--KLRRKITTYVSFPL 497

Query: 508 SLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHKGKLDAGHYVTYL 567
            LDM P+++SS      G    P D    D      +++ LFAV+ H+G L++GHY +++
Sbjct: 498 ELDMTPFMASSKESRMNGQYQQPTDSLNND------NKYSLFAVVNHQGTLESGHYTSFI 525

Query: 568 RL-SNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYK 606
           R   +QW+KCDDA IT+ +   V  ++GY++FY ++ L Y+
Sbjct: 558 RQHKDQWFKCDDAIITKASIKDVLDSEGYLLFYHKQFLEYE 525

BLAST of MC08g2416 vs. ExPASy Swiss-Prot
Match: P0C8Z3 (Ubiquitin carboxyl-terminal hydrolase 22 OS=Bos taurus OX=9913 GN=USP22 PE=2 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 1.0e-87
Identity = 208/581 (35.80%), Postives = 294/581 (50.60%), Query Frame = 0

Query: 28  IFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIRRDPDEVPRCSFCGDSALPRL 87
           + PP C HL  F+  N  ++ R +  C       G A  R+   +   C  CG   L RL
Sbjct: 7   VTPPGCAHLGSFKVDNWKQNLRAIYQCF---VWSGSAEARKRKAKSCVCHVCG-LHLNRL 66

Query: 88  YACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRAELFCCACRDQVYDRDFDAAV 147
           ++C+ C    C    +  H   HA+S    H +A+++    ++C  C+D +YD+D +   
Sbjct: 67  HSCLHCVFFGCF---TKKHIHEHAKS--KRHNLAIELMYGGIYCFLCQDYIYDKDIEIIA 126

Query: 148 VLAQTTASTLGGNVVSSSGAPPQPLLPPENLRKRRRVDYQPWTPDLRERVLMGANSSPLD 207
              Q  A  + G  V                       +  W P  RE  L+  N     
Sbjct: 127 KEEQRKAWKMQG--VGEK--------------------FSTWEPTKRELELLKHNP---- 186

Query: 208 GANSNMGSESEMPSGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQRNVN 267
                    S    GLRGL NLGNTCFMN ++QAL HTP LR+FFLSDRH    C+    
Sbjct: 187 ---KRRKITSNCTIGLRGLINLGNTCFMNCIVQALTHTPLLRDFFLSDRHR---CE---- 246

Query: 268 VGNKNSNSNDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSWWQHAANLASYE 327
                         ++   CL C++ ++F   +SG R+P+ P K L   W HA +LA YE
Sbjct: 247 -------------MQSPSSCLVCEMSSLFQEFYSGHRSPHIPYKLLHLVWTHARHLAGYE 306

Query: 328 QQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDC-CIAHRVFSGILRSDVMCMACGFTST 387
           QQDAHEF I+ L+ +H     D     +     C CI  ++F+G L+SDV C  C   ST
Sbjct: 307 QQDAHEFLIAALDVLHRHCKGDDNGKKANNPNHCNCIIDQIFTGGLQSDVTCQVCHGVST 366

Query: 388 TYDPCVDISLDLEPNLGGSAKMAATRSNLSCNGEADCVN-SSQNHRISSLVGCLDQFTRP 447
           T DP  DISLDL    G S         LS   E+  VN  S     ++L  CL +FTRP
Sbjct: 367 TIDPFWDISLDLP---GSSTPFWP----LSPGSESSVVNGESHVSGTTTLTDCLRRFTRP 426

Query: 448 EKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPF 507
           E LGS  K  C  C   QES KQ++++KLP+V+CFH+KRFEHS+  K+ RK+  Y+ FP 
Sbjct: 427 EHLGSSAKIKCSGCHSYQESTKQLTMKKLPIVACFHLKRFEHSA--KLRRKITTYVSFPL 486

Query: 508 SLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHKGKLDAGHYVTYL 567
            LDM P+++SS      G    P D    D      +++ LFAV+ H+G L++GHY +++
Sbjct: 487 ELDMTPFMASSKESRMNGQYQQPTDSLNND------NKYSLFAVVNHQGTLESGHYTSFI 514

Query: 568 RL-SNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYK 606
           R   +QW+KCDDA IT+ +   V  ++GY++FY ++ L Y+
Sbjct: 547 RQHKDQWFKCDDAIITKASIADVLDSEGYLLFYHKQFLEYE 514

BLAST of MC08g2416 vs. NCBI nr
Match: XP_038889878.1 (ubiquitin C-terminal hydrolase 22-like [Benincasa hispida])

HSP 1 Score: 1169 bits (3025), Expect = 0.0
Identity = 577/614 (93.97%), Postives = 589/614 (95.93%), Query Frame = 0

Query: 1   MSSNIHHHHHHHHHHRHHHRHLPFNAQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPP 60
           MSSN HHHH        HH +LPFN+QI PPSCPHLADFRSRNGSKSFRLLQDCLRVKPP
Sbjct: 1   MSSNFHHHH-------RHHPNLPFNSQISPPSCPHLADFRSRNGSKSFRLLQDCLRVKPP 60

Query: 61  GGRAAIRRDPDEVPRCSFCGDSALPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEI 120
           GGRAAIRRDP+EVPRC  CGDSA PRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEI
Sbjct: 61  GGRAAIRRDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEI 120

Query: 121 AVDVDRAELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNVVSSSG-APPQPLLPPENLR 180
           AVDVDRAELFCCACRDQVYDRDFDAAVVLAQTTASTLGGN VSSSG +P QPLLPPENLR
Sbjct: 121 AVDVDRAELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGVSPSQPLLPPENLR 180

Query: 181 KRRRVDYQPWTPDLRERVLMGANSSPLDGANSNMGSESEMPSGLRGLNNLGNTCFMNSVL 240
           KRRRVDYQPW PDLRERVL+GANSSPLD ANSN+GSESE+P GLRGLNNLGNTCFMNSVL
Sbjct: 181 KRRRVDYQPWIPDLRERVLVGANSSPLDCANSNLGSESELPWGLRGLNNLGNTCFMNSVL 240

Query: 241 QALLHTPPLRNFFLSDRHNRYFCQRNVNVGNKNSNSNDGNGSKNSRICLACDLDAMFSAV 300
           QALLHTPPLRNFFLSDRHNRYFCQRNVN GN NSN+NDGNGSKNSRICLACDLDAMFSAV
Sbjct: 241 QALLHTPPLRNFFLSDRHNRYFCQRNVNGGNINSNANDGNGSKNSRICLACDLDAMFSAV 300

Query: 301 FSGDRTPYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNG 360
           FSGDRTPYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNG
Sbjct: 301 FSGDRTPYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNG 360

Query: 361 DCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAATRSNLSCNG 420
           DCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGGS+KMAA RSNLSCNG
Sbjct: 361 DCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGGSSKMAAARSNLSCNG 420

Query: 421 EADCVNSSQNHRISSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSC 480
           EADC+NSSQN R+SSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSC
Sbjct: 421 EADCMNSSQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSC 480

Query: 481 FHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNE 540
           FHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQD CNE
Sbjct: 481 FHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDGCNE 540

Query: 541 SSSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQ 600
           +SSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQ
Sbjct: 541 TSSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQ 600

Query: 601 KMLYYKASEKQSAS 613
           KMLYYKASEKQSAS
Sbjct: 601 KMLYYKASEKQSAS 607

BLAST of MC08g2416 vs. NCBI nr
Match: XP_022946305.1 (ubiquitin carboxyl-terminal hydrolase 22-like [Cucurbita moschata] >XP_023547055.1 ubiquitin carboxyl-terminal hydrolase 22-like [Cucurbita pepo subsp. pepo] >KAG7030363.1 Ubiquitin carboxyl-terminal hydrolase 22 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1168 bits (3022), Expect = 0.0
Identity = 575/614 (93.65%), Postives = 588/614 (95.77%), Query Frame = 0

Query: 1   MSSNIHHHHHHHHHHRHHHRHLPFNAQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPP 60
           MSSN HHHH        HH +LPFN+QIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPP
Sbjct: 1   MSSNFHHHH-------RHHPNLPFNSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPP 60

Query: 61  GGRAAIRRDPDEVPRCSFCGDSALPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEI 120
           GGRAAIRRDP+EVPRC  CGDSA PRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEI
Sbjct: 61  GGRAAIRRDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEI 120

Query: 121 AVDVDRAELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNVVSSSGAPP-QPLLPPENLR 180
           AVDVDRAELFCCACRDQVYDRDFDAAVVLAQTTASTLGGN VSSSGAPP QPLLPPENLR
Sbjct: 121 AVDVDRAELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLR 180

Query: 181 KRRRVDYQPWTPDLRERVLMGANSSPLDGANSNMGSESEMPSGLRGLNNLGNTCFMNSVL 240
           KRRRVDYQPW PDLRERVL+GANSSPLD ANSN GSESE+P GLRGLNNLGNTCFMNSVL
Sbjct: 181 KRRRVDYQPWVPDLRERVLVGANSSPLDCANSNPGSESELPWGLRGLNNLGNTCFMNSVL 240

Query: 241 QALLHTPPLRNFFLSDRHNRYFCQRNVNVGNKNSNSNDGNGSKNSRICLACDLDAMFSAV 300
           QALLHTPPLRNFFLSDRHNRYFCQRNVNVGNKNSN+NDGNGSKNSR CLACDLD++FSAV
Sbjct: 241 QALLHTPPLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAV 300

Query: 301 FSGDRTPYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNG 360
           FSGDR PYSPAKFL SWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNG
Sbjct: 301 FSGDRIPYSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNG 360

Query: 361 DCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAATRSNLSCNG 420
           DCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNL GSAKMAATRSNL CNG
Sbjct: 361 DCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLAGSAKMAATRSNLPCNG 420

Query: 421 EADCVNSSQNHRISSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSC 480
           EADC+NSSQN R+SSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSC
Sbjct: 421 EADCMNSSQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSC 480

Query: 481 FHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNE 540
           FHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILR+RFGNRIFPFDGDEQD CNE
Sbjct: 481 FHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNE 540

Query: 541 SSSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQ 600
           +SSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQ
Sbjct: 541 TSSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQ 600

Query: 601 KMLYYKASEKQSAS 613
           KMLYYKASEKQ+AS
Sbjct: 601 KMLYYKASEKQNAS 607

BLAST of MC08g2416 vs. NCBI nr
Match: XP_008457196.1 (PREDICTED: ubiquitin carboxyl-terminal hydrolase 22-like [Cucumis melo] >KAA0063520.1 ubiquitin carboxyl-terminal hydrolase 22-like [Cucumis melo var. makuwa] >TYJ97847.1 ubiquitin carboxyl-terminal hydrolase 22-like [Cucumis melo var. makuwa])

HSP 1 Score: 1157 bits (2992), Expect = 0.0
Identity = 565/597 (94.64%), Postives = 578/597 (96.82%), Query Frame = 0

Query: 18  HHRHLPFNAQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIRRDPDEVPRCS 77
           HH +LPF +QIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIRRDP+EVPRC+
Sbjct: 6   HHPNLPFTSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIRRDPNEVPRCA 65

Query: 78  FCGDSALPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRAELFCCACRDQ 137
            CGDSA PRLYACVTCAAVSCHAPPSHSHAAAHAESMP GHEIAVDVDRAELFCCACRDQ
Sbjct: 66  SCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPTGHEIAVDVDRAELFCCACRDQ 125

Query: 138 VYDRDFDAAVVLAQTTASTLGGNVVSSSG-APPQPLLPPENLRKRRRVDYQPWTPDLRER 197
           VYDRDFDAAVVLAQTTASTLGGN VSSSG +P QPLLPPENLRKRRRVDYQPW PDLRER
Sbjct: 126 VYDRDFDAAVVLAQTTASTLGGNAVSSSGVSPSQPLLPPENLRKRRRVDYQPWMPDLRER 185

Query: 198 VLMGANSSPLDGANSNMGSESEMPSGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDR 257
           VL+GANSSPLD  NSN+GSESE+P GLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDR
Sbjct: 186 VLVGANSSPLDCVNSNLGSESELPWGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDR 245

Query: 258 HNRYFCQRNVNVGNKNSNSNDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSW 317
           HNRY+CQRNVN GN NSN NDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSW
Sbjct: 246 HNRYYCQRNVNGGNINSNVNDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSW 305

Query: 318 WQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHRVFSGILRSDV 377
           WQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHRVFSGILRSDV
Sbjct: 306 WQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHRVFSGILRSDV 365

Query: 378 MCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAATRSNLSCNGEADCVNSSQNHRISSLV 437
           MCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAA RSNL CNGEADC+NSSQN R+SSLV
Sbjct: 366 MCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAAARSNLPCNGEADCMNSSQNQRLSSLV 425

Query: 438 GCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRK 497
           GCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRK
Sbjct: 426 GCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRK 485

Query: 498 VDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHKGKL 557
           VDRYLQFPFSLDMAPYLSSSILR+RFGNRIFPFDGDEQD CNE+SSEFELFAVITHKGKL
Sbjct: 486 VDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFELFAVITHKGKL 545

Query: 558 DAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQSAS 613
           DAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQSAS
Sbjct: 546 DAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQSAS 602

BLAST of MC08g2416 vs. NCBI nr
Match: XP_022999050.1 (ubiquitin carboxyl-terminal hydrolase 22-like [Cucurbita maxima])

HSP 1 Score: 1153 bits (2983), Expect = 0.0
Identity = 570/614 (92.83%), Postives = 583/614 (94.95%), Query Frame = 0

Query: 1   MSSNIHHHHHHHHHHRHHHRHLPFNAQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPP 60
           MSSN HHHH        HH +LPFN+QIFP SCPHLADFRSRNGSKSFRLLQDCLRVKPP
Sbjct: 1   MSSNFHHHH-------RHHPNLPFNSQIFPTSCPHLADFRSRNGSKSFRLLQDCLRVKPP 60

Query: 61  GGRAAIRRDPDEVPRCSFCGDSALPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEI 120
           GGRAAIRRDP+EVPRC  CGDSA PRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEI
Sbjct: 61  GGRAAIRRDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEI 120

Query: 121 AVDVDRAELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNVVSSSGAPP-QPLLPPENLR 180
           AVDVDRAELFCCACRDQVYDRDFDAAVVLAQTTASTLGGN VSSSGAPP QPLLPPENLR
Sbjct: 121 AVDVDRAELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLR 180

Query: 181 KRRRVDYQPWTPDLRERVLMGANSSPLDGANSNMGSESEMPSGLRGLNNLGNTCFMNSVL 240
           KRRRVDYQPW PDLRERVL+G NSSPLD ANSN GSES +P GLRGLNNLGNTCFMNSVL
Sbjct: 181 KRRRVDYQPWVPDLRERVLVGTNSSPLDCANSNPGSESALPWGLRGLNNLGNTCFMNSVL 240

Query: 241 QALLHTPPLRNFFLSDRHNRYFCQRNVNVGNKNSNSNDGNGSKNSRICLACDLDAMFSAV 300
           QALLHTPPLRNFFLSDRHNRYFCQRNVNVGNKNSN+NDGNGSKNSR CLACDLD++FSAV
Sbjct: 241 QALLHTPPLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAV 300

Query: 301 FSGDRTPYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNG 360
           FSGDR PYSPAKFL SWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNG
Sbjct: 301 FSGDRIPYSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNG 360

Query: 361 DCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAATRSNLSCNG 420
           DCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPN  GSAKMAATR NL CNG
Sbjct: 361 DCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNQAGSAKMAATR-NLPCNG 420

Query: 421 EADCVNSSQNHRISSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSC 480
           EADC+NSSQN R+SSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSC
Sbjct: 421 EADCMNSSQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSC 480

Query: 481 FHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNE 540
           FHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQD CNE
Sbjct: 481 FHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDGCNE 540

Query: 541 SSSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQ 600
           +SSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQ
Sbjct: 541 TSSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQ 600

Query: 601 KMLYYKASEKQSAS 613
           KMLYYK+SEKQ+AS
Sbjct: 601 KMLYYKSSEKQNAS 606

BLAST of MC08g2416 vs. NCBI nr
Match: XP_004139406.1 (ubiquitin C-terminal hydrolase 22 isoform X2 [Cucumis sativus] >KGN60716.1 hypothetical protein Csa_019516 [Cucumis sativus])

HSP 1 Score: 1150 bits (2976), Expect = 0.0
Identity = 564/597 (94.47%), Postives = 574/597 (96.15%), Query Frame = 0

Query: 18  HHRHLPFNAQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIRRDPDEVPRCS 77
           HH +LPF +QIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIRRDP+EVPRCS
Sbjct: 6   HHPNLPFTSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIRRDPNEVPRCS 65

Query: 78  FCGDSALPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRAELFCCACRDQ 137
            CGDSA PRLYACVTCAAVSCHAPPSHSHAAAHAESMP GHEIAVDVDRAELFCCACRDQ
Sbjct: 66  SCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPTGHEIAVDVDRAELFCCACRDQ 125

Query: 138 VYDRDFDAAVVLAQTTASTLGGNVVSSSG-APPQPLLPPENLRKRRRVDYQPWTPDLRER 197
           VYDRDFDAAVVLAQTTASTLGGN VSSSG +P QP LPPENLRKRRRVDYQPW PDLRER
Sbjct: 126 VYDRDFDAAVVLAQTTASTLGGNAVSSSGVSPSQPSLPPENLRKRRRVDYQPWMPDLRER 185

Query: 198 VLMGANSSPLDGANSNMGSESEMPSGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDR 257
           VL+GANSSPLD  NSN+GSESE+P GLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDR
Sbjct: 186 VLVGANSSPLDCVNSNLGSESELPWGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDR 245

Query: 258 HNRYFCQRNVNVGNKNSNSNDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSW 317
           HNRYFCQRNVN  N +SN NDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSW
Sbjct: 246 HNRYFCQRNVNGSNVSSNVNDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSW 305

Query: 318 WQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHRVFSGILRSDV 377
           WQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHRVFSGILRSDV
Sbjct: 306 WQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHRVFSGILRSDV 365

Query: 378 MCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAATRSNLSCNGEADCVNSSQNHRISSLV 437
           MCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAA RSNL CNGEADC+NSSQN R+SSLV
Sbjct: 366 MCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAAARSNLPCNGEADCMNSSQNQRLSSLV 425

Query: 438 GCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRK 497
           GCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRK
Sbjct: 426 GCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRK 485

Query: 498 VDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHKGKL 557
           VDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDE D CNE SSEFELFAVITHKGKL
Sbjct: 486 VDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDELDGCNEMSSEFELFAVITHKGKL 545

Query: 558 DAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQSAS 613
           DAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQSAS
Sbjct: 546 DAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQSAS 602

BLAST of MC08g2416 vs. ExPASy TrEMBL
Match: A0A6J1G3H3 (Ubiquitin carboxyl-terminal hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111450426 PE=3 SV=1)

HSP 1 Score: 1168 bits (3022), Expect = 0.0
Identity = 575/614 (93.65%), Postives = 588/614 (95.77%), Query Frame = 0

Query: 1   MSSNIHHHHHHHHHHRHHHRHLPFNAQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPP 60
           MSSN HHHH        HH +LPFN+QIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPP
Sbjct: 1   MSSNFHHHH-------RHHPNLPFNSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPP 60

Query: 61  GGRAAIRRDPDEVPRCSFCGDSALPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEI 120
           GGRAAIRRDP+EVPRC  CGDSA PRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEI
Sbjct: 61  GGRAAIRRDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEI 120

Query: 121 AVDVDRAELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNVVSSSGAPP-QPLLPPENLR 180
           AVDVDRAELFCCACRDQVYDRDFDAAVVLAQTTASTLGGN VSSSGAPP QPLLPPENLR
Sbjct: 121 AVDVDRAELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLR 180

Query: 181 KRRRVDYQPWTPDLRERVLMGANSSPLDGANSNMGSESEMPSGLRGLNNLGNTCFMNSVL 240
           KRRRVDYQPW PDLRERVL+GANSSPLD ANSN GSESE+P GLRGLNNLGNTCFMNSVL
Sbjct: 181 KRRRVDYQPWVPDLRERVLVGANSSPLDCANSNPGSESELPWGLRGLNNLGNTCFMNSVL 240

Query: 241 QALLHTPPLRNFFLSDRHNRYFCQRNVNVGNKNSNSNDGNGSKNSRICLACDLDAMFSAV 300
           QALLHTPPLRNFFLSDRHNRYFCQRNVNVGNKNSN+NDGNGSKNSR CLACDLD++FSAV
Sbjct: 241 QALLHTPPLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAV 300

Query: 301 FSGDRTPYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNG 360
           FSGDR PYSPAKFL SWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNG
Sbjct: 301 FSGDRIPYSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNG 360

Query: 361 DCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAATRSNLSCNG 420
           DCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNL GSAKMAATRSNL CNG
Sbjct: 361 DCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLAGSAKMAATRSNLPCNG 420

Query: 421 EADCVNSSQNHRISSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSC 480
           EADC+NSSQN R+SSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSC
Sbjct: 421 EADCMNSSQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSC 480

Query: 481 FHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNE 540
           FHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILR+RFGNRIFPFDGDEQD CNE
Sbjct: 481 FHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNE 540

Query: 541 SSSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQ 600
           +SSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQ
Sbjct: 541 TSSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQ 600

Query: 601 KMLYYKASEKQSAS 613
           KMLYYKASEKQ+AS
Sbjct: 601 KMLYYKASEKQNAS 607

BLAST of MC08g2416 vs. ExPASy TrEMBL
Match: A0A5D3BFE3 (Ubiquitin carboxyl-terminal hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G00590 PE=3 SV=1)

HSP 1 Score: 1157 bits (2992), Expect = 0.0
Identity = 565/597 (94.64%), Postives = 578/597 (96.82%), Query Frame = 0

Query: 18  HHRHLPFNAQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIRRDPDEVPRCS 77
           HH +LPF +QIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIRRDP+EVPRC+
Sbjct: 6   HHPNLPFTSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIRRDPNEVPRCA 65

Query: 78  FCGDSALPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRAELFCCACRDQ 137
            CGDSA PRLYACVTCAAVSCHAPPSHSHAAAHAESMP GHEIAVDVDRAELFCCACRDQ
Sbjct: 66  SCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPTGHEIAVDVDRAELFCCACRDQ 125

Query: 138 VYDRDFDAAVVLAQTTASTLGGNVVSSSG-APPQPLLPPENLRKRRRVDYQPWTPDLRER 197
           VYDRDFDAAVVLAQTTASTLGGN VSSSG +P QPLLPPENLRKRRRVDYQPW PDLRER
Sbjct: 126 VYDRDFDAAVVLAQTTASTLGGNAVSSSGVSPSQPLLPPENLRKRRRVDYQPWMPDLRER 185

Query: 198 VLMGANSSPLDGANSNMGSESEMPSGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDR 257
           VL+GANSSPLD  NSN+GSESE+P GLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDR
Sbjct: 186 VLVGANSSPLDCVNSNLGSESELPWGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDR 245

Query: 258 HNRYFCQRNVNVGNKNSNSNDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSW 317
           HNRY+CQRNVN GN NSN NDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSW
Sbjct: 246 HNRYYCQRNVNGGNINSNVNDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSW 305

Query: 318 WQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHRVFSGILRSDV 377
           WQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHRVFSGILRSDV
Sbjct: 306 WQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHRVFSGILRSDV 365

Query: 378 MCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAATRSNLSCNGEADCVNSSQNHRISSLV 437
           MCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAA RSNL CNGEADC+NSSQN R+SSLV
Sbjct: 366 MCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAAARSNLPCNGEADCMNSSQNQRLSSLV 425

Query: 438 GCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRK 497
           GCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRK
Sbjct: 426 GCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRK 485

Query: 498 VDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHKGKL 557
           VDRYLQFPFSLDMAPYLSSSILR+RFGNRIFPFDGDEQD CNE+SSEFELFAVITHKGKL
Sbjct: 486 VDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFELFAVITHKGKL 545

Query: 558 DAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQSAS 613
           DAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQSAS
Sbjct: 546 DAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQSAS 602

BLAST of MC08g2416 vs. ExPASy TrEMBL
Match: A0A1S3C4I9 (Ubiquitin carboxyl-terminal hydrolase OS=Cucumis melo OX=3656 GN=LOC103496929 PE=3 SV=1)

HSP 1 Score: 1157 bits (2992), Expect = 0.0
Identity = 565/597 (94.64%), Postives = 578/597 (96.82%), Query Frame = 0

Query: 18  HHRHLPFNAQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIRRDPDEVPRCS 77
           HH +LPF +QIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIRRDP+EVPRC+
Sbjct: 6   HHPNLPFTSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIRRDPNEVPRCA 65

Query: 78  FCGDSALPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRAELFCCACRDQ 137
            CGDSA PRLYACVTCAAVSCHAPPSHSHAAAHAESMP GHEIAVDVDRAELFCCACRDQ
Sbjct: 66  SCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPTGHEIAVDVDRAELFCCACRDQ 125

Query: 138 VYDRDFDAAVVLAQTTASTLGGNVVSSSG-APPQPLLPPENLRKRRRVDYQPWTPDLRER 197
           VYDRDFDAAVVLAQTTASTLGGN VSSSG +P QPLLPPENLRKRRRVDYQPW PDLRER
Sbjct: 126 VYDRDFDAAVVLAQTTASTLGGNAVSSSGVSPSQPLLPPENLRKRRRVDYQPWMPDLRER 185

Query: 198 VLMGANSSPLDGANSNMGSESEMPSGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDR 257
           VL+GANSSPLD  NSN+GSESE+P GLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDR
Sbjct: 186 VLVGANSSPLDCVNSNLGSESELPWGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDR 245

Query: 258 HNRYFCQRNVNVGNKNSNSNDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSW 317
           HNRY+CQRNVN GN NSN NDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSW
Sbjct: 246 HNRYYCQRNVNGGNINSNVNDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSW 305

Query: 318 WQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHRVFSGILRSDV 377
           WQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHRVFSGILRSDV
Sbjct: 306 WQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHRVFSGILRSDV 365

Query: 378 MCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAATRSNLSCNGEADCVNSSQNHRISSLV 437
           MCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAA RSNL CNGEADC+NSSQN R+SSLV
Sbjct: 366 MCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAAARSNLPCNGEADCMNSSQNQRLSSLV 425

Query: 438 GCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRK 497
           GCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRK
Sbjct: 426 GCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRK 485

Query: 498 VDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHKGKL 557
           VDRYLQFPFSLDMAPYLSSSILR+RFGNRIFPFDGDEQD CNE+SSEFELFAVITHKGKL
Sbjct: 486 VDRYLQFPFSLDMAPYLSSSILRNRFGNRIFPFDGDEQDGCNETSSEFELFAVITHKGKL 545

Query: 558 DAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQSAS 613
           DAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQSAS
Sbjct: 546 DAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQSAS 602

BLAST of MC08g2416 vs. ExPASy TrEMBL
Match: A0A6J1KE79 (Ubiquitin carboxyl-terminal hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111493554 PE=3 SV=1)

HSP 1 Score: 1153 bits (2983), Expect = 0.0
Identity = 570/614 (92.83%), Postives = 583/614 (94.95%), Query Frame = 0

Query: 1   MSSNIHHHHHHHHHHRHHHRHLPFNAQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPP 60
           MSSN HHHH        HH +LPFN+QIFP SCPHLADFRSRNGSKSFRLLQDCLRVKPP
Sbjct: 1   MSSNFHHHH-------RHHPNLPFNSQIFPTSCPHLADFRSRNGSKSFRLLQDCLRVKPP 60

Query: 61  GGRAAIRRDPDEVPRCSFCGDSALPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEI 120
           GGRAAIRRDP+EVPRC  CGDSA PRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEI
Sbjct: 61  GGRAAIRRDPNEVPRCGSCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEI 120

Query: 121 AVDVDRAELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNVVSSSGAPP-QPLLPPENLR 180
           AVDVDRAELFCCACRDQVYDRDFDAAVVLAQTTASTLGGN VSSSGAPP QPLLPPENLR
Sbjct: 121 AVDVDRAELFCCACRDQVYDRDFDAAVVLAQTTASTLGGNAVSSSGAPPSQPLLPPENLR 180

Query: 181 KRRRVDYQPWTPDLRERVLMGANSSPLDGANSNMGSESEMPSGLRGLNNLGNTCFMNSVL 240
           KRRRVDYQPW PDLRERVL+G NSSPLD ANSN GSES +P GLRGLNNLGNTCFMNSVL
Sbjct: 181 KRRRVDYQPWVPDLRERVLVGTNSSPLDCANSNPGSESALPWGLRGLNNLGNTCFMNSVL 240

Query: 241 QALLHTPPLRNFFLSDRHNRYFCQRNVNVGNKNSNSNDGNGSKNSRICLACDLDAMFSAV 300
           QALLHTPPLRNFFLSDRHNRYFCQRNVNVGNKNSN+NDGNGSKNSR CLACDLD++FSAV
Sbjct: 241 QALLHTPPLRNFFLSDRHNRYFCQRNVNVGNKNSNANDGNGSKNSRSCLACDLDSIFSAV 300

Query: 301 FSGDRTPYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNG 360
           FSGDR PYSPAKFL SWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNG
Sbjct: 301 FSGDRIPYSPAKFLLSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNG 360

Query: 361 DCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAATRSNLSCNG 420
           DCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPN  GSAKMAATR NL CNG
Sbjct: 361 DCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNQAGSAKMAATR-NLPCNG 420

Query: 421 EADCVNSSQNHRISSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSC 480
           EADC+NSSQN R+SSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSC
Sbjct: 421 EADCMNSSQNQRLSSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSC 480

Query: 481 FHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNE 540
           FHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQD CNE
Sbjct: 481 FHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDGCNE 540

Query: 541 SSSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQ 600
           +SSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQ
Sbjct: 541 TSSEFELFAVITHKGKLDAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQ 600

Query: 601 KMLYYKASEKQSAS 613
           KMLYYK+SEKQ+AS
Sbjct: 601 KMLYYKSSEKQNAS 606

BLAST of MC08g2416 vs. ExPASy TrEMBL
Match: A0A0A0LL31 (Ubiquitin carboxyl-terminal hydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G008080 PE=3 SV=1)

HSP 1 Score: 1150 bits (2976), Expect = 0.0
Identity = 564/597 (94.47%), Postives = 574/597 (96.15%), Query Frame = 0

Query: 18  HHRHLPFNAQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIRRDPDEVPRCS 77
           HH +LPF +QIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIRRDP+EVPRCS
Sbjct: 6   HHPNLPFTSQIFPPSCPHLADFRSRNGSKSFRLLQDCLRVKPPGGRAAIRRDPNEVPRCS 65

Query: 78  FCGDSALPRLYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRAELFCCACRDQ 137
            CGDSA PRLYACVTCAAVSCHAPPSHSHAAAHAESMP GHEIAVDVDRAELFCCACRDQ
Sbjct: 66  SCGDSARPRLYACVTCAAVSCHAPPSHSHAAAHAESMPTGHEIAVDVDRAELFCCACRDQ 125

Query: 138 VYDRDFDAAVVLAQTTASTLGGNVVSSSG-APPQPLLPPENLRKRRRVDYQPWTPDLRER 197
           VYDRDFDAAVVLAQTTASTLGGN VSSSG +P QP LPPENLRKRRRVDYQPW PDLRER
Sbjct: 126 VYDRDFDAAVVLAQTTASTLGGNAVSSSGVSPSQPSLPPENLRKRRRVDYQPWMPDLRER 185

Query: 198 VLMGANSSPLDGANSNMGSESEMPSGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDR 257
           VL+GANSSPLD  NSN+GSESE+P GLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDR
Sbjct: 186 VLVGANSSPLDCVNSNLGSESELPWGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDR 245

Query: 258 HNRYFCQRNVNVGNKNSNSNDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSW 317
           HNRYFCQRNVN  N +SN NDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSW
Sbjct: 246 HNRYFCQRNVNGSNVSSNVNDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSW 305

Query: 318 WQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHRVFSGILRSDV 377
           WQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHRVFSGILRSDV
Sbjct: 306 WQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDCCIAHRVFSGILRSDV 365

Query: 378 MCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAATRSNLSCNGEADCVNSSQNHRISSLV 437
           MCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAA RSNL CNGEADC+NSSQN R+SSLV
Sbjct: 366 MCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAAARSNLPCNGEADCMNSSQNQRLSSLV 425

Query: 438 GCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRK 497
           GCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRK
Sbjct: 426 GCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRK 485

Query: 498 VDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHKGKL 557
           VDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDE D CNE SSEFELFAVITHKGKL
Sbjct: 486 VDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDELDGCNEMSSEFELFAVITHKGKL 545

Query: 558 DAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQSAS 613
           DAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQSAS
Sbjct: 546 DAGHYVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQSAS 602

BLAST of MC08g2416 vs. TAIR 10
Match: AT5G10790.1 (ubiquitin-specific protease 22 )

HSP 1 Score: 518.8 bits (1335), Expect = 5.7e-147
Identity = 293/591 (49.58%), Postives = 372/591 (62.94%), Query Frame = 0

Query: 30  PPSCPHLADFRSR---NGSKSFRLLQDCLRVKPPGGRAAIRRDPDEVPRCSFCGDSALPR 89
           P  C HL+D++ R   +G KSF  L  C        R  I+    ++PRCS+C      R
Sbjct: 11  PDPCNHLSDYKLRYGTDGYKSFNNLFRCFN----DARIKIKLQGIDIPRCSYC-SVYQKR 70

Query: 90  LYACVTCAAVSCHAPPSHSHAAAHAESMPPGHEIAVDVDRAELFCCACRDQVYDRDFDAA 149
           LY C+ C ++SC      SH   H + +  GH+IA+DV+R+EL+CCAC DQVYD +FD  
Sbjct: 71  LYICLICRSISC-----SSHILLHTQ-LNKGHDIAIDVERSELYCCACIDQVYDSEFDEV 130

Query: 150 VVLAQTTASTLGGNVVSSSGAPPQPLLPPENLRKRRRVDYQPWTPDLRERVLMGAN--SS 209
           VV  Q      G  +   SGA     +      K+RR+D Q         +++G+N   S
Sbjct: 131 VVSKQ----LFGLGMSVKSGAD----VVAVRSNKKRRLDSQ---------LIIGSNFLVS 190

Query: 210 PLDGANSNMGSESEMPSGLRGLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQR 269
           P D        +   P GLRGLNNLG+TCFMN+VLQAL+H PPLRNF+LS +HNR  C R
Sbjct: 191 PRD-----RREKWTFPLGLRGLNNLGSTCFMNAVLQALVHAPPLRNFWLSGQHNRDLCPR 250

Query: 270 NVNVGNKNSNSNDGNGSKNSRICLACDLDAMFSAVFSGDRTPYSPAKFLFSWWQHAANLA 329
                                +CL CDLD +FSA+FSGDRTPYSPA  L+SWWQH+ NLA
Sbjct: 251 RT----------------MGLLCLPCDLDVIFSAMFSGDRTPYSPAHLLYSWWQHSTNLA 310

Query: 330 SYEQQDAHEFFISILEGIHEKVDKDRRKPYSQGNGDC-CIAHRVFSGILRSDVMCMACGF 389
           +YEQQD+HEFFIS+L+ IHE  ++ + K   Q N +C CI H+ FSG+LRSDV C  CG 
Sbjct: 311 TYEQQDSHEFFISLLDRIHE--NEGKSKCLYQDNEECQCITHKAFSGLLRSDVTCTTCGS 370

Query: 390 TSTTYDPCVDISLDLEPNLGGSAKMAATRSNLSCNGEADCVNSSQNHRISSLVGCLDQFT 449
           TSTTYDP +DISL L+   G S   A  R N    G       S N  + +L GCLD FT
Sbjct: 371 TSTTYDPFIDISLTLDSMNGFSP--ADCRKNRYSGG------PSVNAIMPTLSGCLDFFT 430

Query: 450 RPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQF 509
           R EKLG DQK  CQ C  ++ES KQMSIR+LPL+ C H+KRFEHS  RK SRK+D YLQ+
Sbjct: 431 RSEKLGPDQKLNCQSCGEKRESSKQMSIRRLPLLLCLHVKRFEHSLTRKTSRKIDSYLQY 490

Query: 510 PFSLDMAPYLSSSILRSRFGNRIFPFDGD-EQDACNES--SSEFELFAVITHKGKLDAGH 569
           PF L+M+PYLSSSI+  RFGNRIF FDG+ E D+ + S  S+EFE+FAV+THKG L++GH
Sbjct: 491 PFRLNMSPYLSSSIIGKRFGNRIFAFDGEGEYDSSSSSSPSAEFEIFAVVTHKGMLESGH 542

Query: 570 YVTYLRLSNQWYKCDDAWITQVNENIVRAAQGYMMFYVQKMLYYKASEKQS 612
           YVTYLRL   WY+CDDAWI +V E +VR  + YM+FY Q+ +  KA ++ S
Sbjct: 551 YVTYLRLKGLWYRCDDAWINEVEEEVVRGCECYMLFYAQETVIQKAHKELS 542

BLAST of MC08g2416 vs. TAIR 10
Match: AT5G57990.1 (ubiquitin-specific protease 23 )

HSP 1 Score: 146.4 bits (368), Expect = 7.7e-35
Identity = 104/383 (27.15%), Postives = 166/383 (43.34%), Query Frame = 0

Query: 225 GLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQRNVNVGNKNSNSNDGNGSKNS 284
           GL NLGNTCF+NSVLQ L +T PL     +  H +Y                       +
Sbjct: 108 GLQNLGNTCFLNSVLQCLTYTEPLAATLQTAAHQKY--------------------CHVA 167

Query: 285 RICLACDLDAMFSAVFSGDRTPYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHE 344
             C  C +          +    +P   + +    + N  +  Q+DAHE+ I++LE +H+
Sbjct: 168 GFCALCAIQKHVRTARQANGRILAPKDLVSNLRCISRNFRNCRQEDAHEYMINLLECMHK 227

Query: 345 -----KVDKDRRKPYSQGNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLE 404
                 V  +    Y +      + H++F G LRS V C  C   S  +DP +D+SLD+ 
Sbjct: 228 CSLPSGVPSESSDAYRRS-----LVHKIFGGSLRSQVKCEQCSHCSNKFDPFLDLSLDIS 287

Query: 405 PNLGGSAKMAATRSNLSCNGEADCVNSSQNHRISSLVGCLDQFTRPEKLGSDQKFF-CQQ 464
                                          +  SL   L +FT  E L +  K + C++
Sbjct: 288 -------------------------------KADSLQRALSRFTAVELLDNGAKVYQCER 347

Query: 465 CQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSIL 524
           C+ + ++ KQ+++ K P V   H+KRFE     K+ RKVD    F  ++DM P++S    
Sbjct: 348 CKQKVKAKKQLTVSKAPYVLTVHLKRFEAHRSEKIDRKVD----FTSAIDMKPFVSGP-- 407

Query: 525 RSRFGNRIFPFDGDEQDACNESSSEFELFAVITHKGKLD-AGHYVTYLRLSN-QWYKCDD 584
                              +E + ++ L+ V+ H G+   +GHY  ++R S+  WY  DD
Sbjct: 408 -------------------HEGNLKYTLYGVLVHYGRSSHSGHYACFVRTSSGMWYSLDD 409

Query: 585 AWITQVNENIVRAAQGYMMFYVQ 600
             + QV+E  V   + YM+FYV+
Sbjct: 468 NRVVQVSEKTVFNQKAYMLFYVR 409

BLAST of MC08g2416 vs. TAIR 10
Match: AT4G17895.1 (ubiquitin-specific protease 20 )

HSP 1 Score: 141.4 bits (355), Expect = 2.5e-33
Identity = 115/426 (27.00%), Postives = 195/426 (45.77%), Query Frame = 0

Query: 187 QPWTPDLRERVLMGANSSPLDGANSN---------MGSESEMPSGL-RGLNNLGNTCFMN 246
           +PWTP++        +    D  + N            E E  +G+  GL NLGN+CF+N
Sbjct: 129 RPWTPNVSPGFGSDDDDDNDDDNSKNEPRKSLFYGFRQEPEPVTGVGAGLWNLGNSCFLN 188

Query: 247 SVLQALLHTPPLRNFFLSDRHNRYFCQRNVNVGNKNSNSNDGNGSKNSRICLACDLDAMF 306
           SV Q   HT PL    LS R+     +   + GN+               C+   +    
Sbjct: 189 SVFQCFTHTVPLIESLLSFRY-----EVPCHCGNE-------------FFCVIRAIRYHI 248

Query: 307 SAVFSGDRTPYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHEKVDKDRRKPYSQ 366
            A    +R P +P  F  +    + +   Y+Q+DAHEF  + LE + E    DR    + 
Sbjct: 249 EAALRPERCPIAPYFFFDNLNYFSPDFQRYQQEDAHEFLQAFLEKL-EICGSDR----TS 308

Query: 367 GNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPNLGGSAKMAATRSNLS 426
             GD   +  VFSG L S + C  C + S TY+  V +SL++E                 
Sbjct: 309 FRGD-ITSQDVFSGRLISGLRCCNCDYVSETYEKSVGLSLEIED---------------- 368

Query: 427 CNGEADCVNSSQNHRISSLVGCLDQFTRPEKLGSDQKFFCQQCQVRQESLKQMSIRKLPL 486
                          + +L   L+ FTR EKL  D++  C  C  +    KQ+ + KLPL
Sbjct: 369 ---------------VDTLGSALESFTRVEKL--DEQLTCDNCNEKVSKEKQLLLDKLPL 428

Query: 487 VSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSRFGNRIFPFDGDEQDA 546
           V+ FH+KRF+++ +     K+ ++++ P  +D+ PY+ +                  Q+ 
Sbjct: 429 VATFHLKRFKNNGL--YMEKIYKHVKIPLEIDLQPYMRNI-----------------QE- 476

Query: 547 CNESSSEFELFAVITHKG-KLDAGHYVTYLRLSNQ-WYKCDDAWITQVNENIVRAAQGYM 601
            NE S+++ L+A++ H G  +  GHY +Y+R + + W+  DD+ +T+++E++V +   Y+
Sbjct: 489 -NEVSTKYHLYALVEHFGYSVAYGHYSSYVRSAPKIWHHFDDSKVTRIDEDMVLSQDSYI 476

BLAST of MC08g2416 vs. TAIR 10
Match: AT3G14400.1 (ubiquitin-specific protease 25 )

HSP 1 Score: 131.3 bits (329), Expect = 2.6e-30
Identity = 100/378 (26.46%), Postives = 166/378 (43.92%), Query Frame = 0

Query: 225 GLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQRNVNVGNKNSNSNDGNGSKNS 284
           GL NLGNTC++NSVLQ L  TPPL NF L+ +H+ + C   V+              +  
Sbjct: 25  GLRNLGNTCYLNSVLQCLTFTPPLANFCLTHKHSSH-CDTYVD-------------GERK 84

Query: 285 RICLACDLDAMFSAVFSGDRTPYSPAKFLFSWWQHAANLASYEQQDAHEFFISILEGIHE 344
           R C  C ++   +   S D T  +P K        A +     Q+DAHEF   +++  H 
Sbjct: 85  RDCPFCIVEKRIARSLSVDLTTDAPNKISSCLKIFAEHFKLGRQEDAHEFLRYVIDACHN 144

Query: 345 ---KVDKDRRKPYSQGNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDISLDLEPN 404
              ++ K R       NG+  +   +F G L+S V C++CG  S   D  +DISL++  +
Sbjct: 145 TSLRLKKLRYNGNEPFNGN-SVVKEIFGGALQSQVKCLSCGAESNKADEIMDISLEILQS 204

Query: 405 LGGSAKMAATRSNLSCNGEADCVNSSQNHRISSLVGCLDQFTRPEKLGSDQKFFCQQCQV 464
                                          SS+   L +F + E L  + K+ C+ C+ 
Sbjct: 205 -------------------------------SSVKESLQKFFQSEILDGNNKYRCESCEK 264

Query: 465 RQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSSSILRSR 524
              + KQMSI + P +    +KRF          K+D+ + F   L ++ ++S       
Sbjct: 265 LVTARKQMSILQAPNILVIQLKRFGGI----FGGKIDKAISFGEILVLSNFMSK------ 324

Query: 525 FGNRIFPFDGDEQDACNESSSEFELFAVITHKG-KLDAGHYVTYLRLS-NQWYKCDDAWI 584
                         A  +   E++LF +I H G   ++GHY  Y++ S  +WY C+D+++
Sbjct: 325 --------------ASKDPQPEYKLFGIIVHSGFSPESGHYYAYVKDSLGRWYCCNDSFV 332

Query: 585 TQVNENIVRAAQGYMMFY 598
           +      V + + Y++F+
Sbjct: 385 SLSTLQEVLSEKAYILFF 332

BLAST of MC08g2416 vs. TAIR 10
Match: AT2G24640.1 (ubiquitin-specific protease 19 )

HSP 1 Score: 125.9 bits (315), Expect = 1.1e-28
Identity = 103/386 (26.68%), Postives = 162/386 (41.97%), Query Frame = 0

Query: 225 GLNNLGNTCFMNSVLQALLHTPPLRNFFLSDRHNRYFCQRNVNVGNKNSNSNDGNGSKNS 284
           GL N GN+CF N VLQ L  T PL  + L   H R  C+RN                   
Sbjct: 175 GLTNCGNSCFANVVLQCLSWTRPLVAYLLERGHKRE-CRRN------------------- 234

Query: 285 RICLACDLDAMFSAVFSGDRTPYSPAKFLFSWWQHAANLASYEQQDAHE---FFISILEG 344
             C  C+ +       +  R P+SP   +        NL    Q+DAHE   F I +++ 
Sbjct: 235 DWCFLCEFENHLDRA-NYSRFPFSPMNIISRLPNIGGNLGYGRQEDAHELMRFAIDMMQS 294

Query: 345 I------HEKVDKDRRKPYSQGNGDCCIAHRVFSGILRSDVMCMACGFTSTTYDPCVDIS 404
           +       EKV   R +       +  +   +F G+L+S V C AC   S  Y+  +D++
Sbjct: 295 VCLDEFGGEKVVPPRAQ-------ETTLIQYIFGGLLQSQVQCTACSNVSDQYENMMDLT 354

Query: 405 LDLEPNLGGSAKMAATRSNLSCNGEADCVNSSQNHRISSLVGCLDQFTRPEKLGSDQKFF 464
           +++                   +G+A            SL  CLDQFT  E L  D  + 
Sbjct: 355 VEI-------------------HGDA-----------VSLEECLDQFTAKEWLQGDNLYK 414

Query: 465 CQQCQVRQESLKQMSIRKLPLVSCFHIKRFEHSSVRKMSRKVDRYLQFPFSLDMAPYLSS 524
           C +C    ++ K++SIR  P +    +KRF+     K+++++     FP + D+ PY+S 
Sbjct: 415 CDRCDDYVKACKRLSIRCAPNILTIALKRFQGGRFGKLNKRI----SFPETFDLGPYMSG 474

Query: 525 SILRSRFGNRIFPFDGDEQDACNESSSEFELFAVITHKGKLDA---GHYVTYLR-LSNQW 584
                                  E S  ++L+AVI H   L+A   GHY+ Y++     W
Sbjct: 475 G---------------------GEGSDVYKLYAVIVHLDMLNASFFGHYICYVKDFRGNW 477

Query: 585 YKCDDAWITQVNENIVRAAQGYMMFY 598
           Y+ DD+ + +V    V + + YM+ Y
Sbjct: 535 YRIDDSEVEKVELEDVLSQRAYMLLY 477