MC08g2354 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g2354
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionVQ domain-containing protein
LocationMC08: 32393596 .. 32393901 (+)
RNA-Seq ExpressionMC08g2354
SyntenyMC08g2354
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAAAGTGTGGAGAGATGAACCAATACAAGAATCATAGCCAGAGCCAAAACACAACCAAAGTTCAGAGAAAACCCATAAAAGTAAAGTACATTTCAAGCCCAATGATGGTGAAAGCAAGCAATGCAATGGACTTCAGAGCCATTGTTCAGCAACTCACTGGCTTCAACTCCGACGCCACGATTTCTCCGACGAACCACCGACCACCTCCCGCCACCCGAGCTCCGTCTTCAACGTTGCTTTTGGAAGCTATCGGAGTCCCAGACGACAGGTCGTCGTTGCTCCATCTAGATGAAGGTGGTTGT

mRNA sequence

ATGGGAAAGTGTGGAGAGATGAACCAATACAAGAATCATAGCCAGAGCCAAAACACAACCAAAGTTCAGAGAAAACCCATAAAAGTAAAGTACATTTCAAGCCCAATGATGGTGAAAGCAAGCAATGCAATGGACTTCAGAGCCATTGTTCAGCAACTCACTGGCTTCAACTCCGACGCCACGATTTCTCCGACGAACCACCGACCACCTCCCGCCACCCGAGCTCCGTCTTCAACGTTGCTTTTGGAAGCTATCGGAGTCCCAGACGACAGGTCGTCGTTGCTCCATCTAGATGAAGGTGGTTGT

Coding sequence (CDS)

ATGGGAAAGTGTGGAGAGATGAACCAATACAAGAATCATAGCCAGAGCCAAAACACAACCAAAGTTCAGAGAAAACCCATAAAAGTAAAGTACATTTCAAGCCCAATGATGGTGAAAGCAAGCAATGCAATGGACTTCAGAGCCATTGTTCAGCAACTCACTGGCTTCAACTCCGACGCCACGATTTCTCCGACGAACCACCGACCACCTCCCGCCACCCGAGCTCCGTCTTCAACGTTGCTTTTGGAAGCTATCGGAGTCCCAGACGACAGGTCGTCGTTGCTCCATCTAGATGAAGGTGGTTGT

Protein sequence

MGKCGEMNQYKNHSQSQNTTKVQRKPIKVKYISSPMMVKASNAMDFRAIVQQLTGFNSDATISPTNHRPPPATRAPSSTLLLEAIGVPDDRSSLLHLDEGGC
Homology
BLAST of MC08g2354 vs. NCBI nr
Match: KAG6599333.1 (hypothetical protein SDJN03_09111, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 116 bits (291), Expect = 4.41e-31
Identity = 65/108 (60.19%), Postives = 81/108 (75.00%), Query Frame = 0

Query: 1   MGKCGEMNQYKNHSQSQNTTKVQRKPIKVKYISSPMMVKASNAMDFRAIVQQLTGFNSDA 60
           MGKCGE N++K+  QSQN TKVQRKPIKVKYISSPMM+KASNA +FRAIVQQLTGF S +
Sbjct: 1   MGKCGE-NEWKDQKQSQNPTKVQRKPIKVKYISSPMMIKASNAHEFRAIVQQLTGFTSHS 60

Query: 61  TISPTNHRPPP-ATRAPSSTLL-----LEAIGVPDDRSSLLHLDEGGC 102
               +NH+PPP A + PS++ L     +EA+ + D+ SSLL  D+  C
Sbjct: 61  PTRRSNHKPPPHAAQTPSTSTLHANPQMEALKLSDNMSSLLEFDDEAC 107

BLAST of MC08g2354 vs. NCBI nr
Match: KGN60779.1 (hypothetical protein Csa_019238 [Cucumis sativus])

HSP 1 Score: 95.9 bits (237), Expect = 9.04e-23
Identity = 65/119 (54.62%), Postives = 75/119 (63.03%), Query Frame = 0

Query: 1   MGKCGEMNQYKNH------SQSQNTTKVQRKPIKVKYISSPMMVKASNAMDFRAIVQQLT 60
           MGKCGE NQY NH      SQ+QN TKVQRKPIKVKYISSPMM+KASNA++FRAIVQQLT
Sbjct: 1   MGKCGE-NQYTNHNHSQSQSQNQNPTKVQRKPIKVKYISSPMMIKASNALEFRAIVQQLT 60

Query: 61  GFNSDATISPTNHRPPP----ATRAPSSTLL-----LEAIGVPDDR--SSLLHLDEGGC 102
           GF+S+   +PT  R         R PS+        +E +   DD    SLL  D  GC
Sbjct: 61  GFDSN---TPTRRRNQKLLSDCARTPSAMFHADRHEMEVLKFSDDHHMESLLQFDGQGC 115

BLAST of MC08g2354 vs. NCBI nr
Match: KAF3969566.1 (hypothetical protein CMV_006646 [Castanea mollissima])

HSP 1 Score: 67.0 bits (162), Expect = 1.46e-11
Identity = 40/64 (62.50%), Postives = 49/64 (76.56%), Query Frame = 0

Query: 2  GKCGEMNQYKNH-SQSQNTTKVQRKPIKVKYISSPMMVKASNAMDFRAIVQQLTGFNSDA 61
          GK    N Y+N   +S ++TKV+RKP+KVKYISSP+MVKA N  +FRAIVQQLTG N+D 
Sbjct: 4  GKHHVDNHYQNQPKRSPDSTKVERKPMKVKYISSPIMVKAGNPSEFRAIVQQLTGKNADP 63

Query: 62 TISP 64
           ISP
Sbjct: 64 -ISP 66

BLAST of MC08g2354 vs. NCBI nr
Match: XP_021282108.1 (uncharacterized protein LOC110414990 [Herrania umbratica])

HSP 1 Score: 67.4 bits (163), Expect = 1.48e-11
Identity = 35/55 (63.64%), Postives = 45/55 (81.82%), Query Frame = 0

Query: 5  GEMNQYKNHSQSQNTTKVQRKPIKVKYISSPMMVKASNAMDFRAIVQQLTGFNSD 59
          G++  ++ H  S+N TK ++ PIK+KYISSPMMVKASNA +FRAIVQ+LTG NSD
Sbjct: 21 GKLRDHRCHK-SKNPTKAKKNPIKIKYISSPMMVKASNADEFRAIVQELTGRNSD 74

BLAST of MC08g2354 vs. NCBI nr
Match: XP_011468362.1 (PREDICTED: uncharacterized protein LOC105352572 [Fragaria vesca subsp. vesca])

HSP 1 Score: 65.5 bits (158), Expect = 5.96e-11
Identity = 35/61 (57.38%), Postives = 46/61 (75.41%), Query Frame = 0

Query: 7  MNQYKNHSQSQNTTKVQRK-----PIKVKYISSPMMVKASNAMDFRAIVQQLTGFNSDAT 62
          M Q +NH   Q  T+ +++     PI++KYISSPMMV+A+NA +FRAIVQ+LTG NSDAT
Sbjct: 1  MEQLRNHENRQQRTERRKRSKAETPIRIKYISSPMMVQANNASEFRAIVQELTGQNSDAT 60

BLAST of MC08g2354 vs. ExPASy TrEMBL
Match: A0A0A0LIA7 (VQ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G010150 PE=4 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 4.38e-23
Identity = 65/119 (54.62%), Postives = 75/119 (63.03%), Query Frame = 0

Query: 1   MGKCGEMNQYKNH------SQSQNTTKVQRKPIKVKYISSPMMVKASNAMDFRAIVQQLT 60
           MGKCGE NQY NH      SQ+QN TKVQRKPIKVKYISSPMM+KASNA++FRAIVQQLT
Sbjct: 1   MGKCGE-NQYTNHNHSQSQSQNQNPTKVQRKPIKVKYISSPMMIKASNALEFRAIVQQLT 60

Query: 61  GFNSDATISPTNHRPPP----ATRAPSSTLL-----LEAIGVPDDR--SSLLHLDEGGC 102
           GF+S+   +PT  R         R PS+        +E +   DD    SLL  D  GC
Sbjct: 61  GFDSN---TPTRRRNQKLLSDCARTPSAMFHADRHEMEVLKFSDDHHMESLLQFDGQGC 115

BLAST of MC08g2354 vs. ExPASy TrEMBL
Match: A0A6J1A533 (uncharacterized protein LOC110414990 OS=Herrania umbratica OX=108875 GN=LOC110414990 PE=4 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 7.16e-12
Identity = 35/55 (63.64%), Postives = 45/55 (81.82%), Query Frame = 0

Query: 5  GEMNQYKNHSQSQNTTKVQRKPIKVKYISSPMMVKASNAMDFRAIVQQLTGFNSD 59
          G++  ++ H  S+N TK ++ PIK+KYISSPMMVKASNA +FRAIVQ+LTG NSD
Sbjct: 21 GKLRDHRCHK-SKNPTKAKKNPIKIKYISSPMMVKASNADEFRAIVQELTGRNSD 74

BLAST of MC08g2354 vs. ExPASy TrEMBL
Match: A0A5D2MMS7 (VQ domain-containing protein OS=Gossypium tomentosum OX=34277 GN=ES332_A13G204600v1 PE=4 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 1.00e-10
Identity = 45/85 (52.94%), Postives = 52/85 (61.18%), Query Frame = 0

Query: 16  SQNTTKVQRKPIKVKYISSPMMVKASNAMDFRAIVQQLTGFNSDATISPTNHRPPPATRA 75
           S+N  K+   PIKVKYISSPMMVKASNA +FRAIVQ+LTG +SD    P N         
Sbjct: 12  SKNPKKI---PIKVKYISSPMMVKASNAEEFRAIVQELTGQHSDMG-EPVNVVTTNTKAK 71

Query: 76  PSSTLLLEAIGVPDDRSSLLHLDEG 100
                LL+A   PDD SS+   DEG
Sbjct: 72  LDDRYLLDAS--PDDMSSMELFDEG 90

BLAST of MC08g2354 vs. ExPASy TrEMBL
Match: A0A067DJ56 (VQ domain-containing protein OS=Citrus sinensis OX=2711 GN=CISIN_1g031928mg PE=4 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.21e-10
Identity = 35/61 (57.38%), Postives = 46/61 (75.41%), Query Frame = 0

Query: 1  MGKCGEMNQYKNHSQSQNT--TKVQRKPIKVKYISSPMMVKASNAMDFRAIVQQLTGFNS 59
          MGK G    ++ H++SQ +   KV++ P+KV YISSPM VKASNA +FRAIVQ+LTG NS
Sbjct: 1  MGKFGHHFDHQGHNKSQRSGANKVKKNPLKVTYISSPMKVKASNASEFRAIVQELTGKNS 60

BLAST of MC08g2354 vs. ExPASy TrEMBL
Match: A0A2H5QH96 (VQ domain-containing protein OS=Citrus unshiu OX=55188 GN=CUMW_230020 PE=4 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.21e-10
Identity = 35/61 (57.38%), Postives = 46/61 (75.41%), Query Frame = 0

Query: 1  MGKCGEMNQYKNHSQSQNT--TKVQRKPIKVKYISSPMMVKASNAMDFRAIVQQLTGFNS 59
          MGK G    ++ H++SQ +   KV++ P+KV YISSPM VKASNA +FRAIVQ+LTG NS
Sbjct: 1  MGKFGHHFDHQGHNKSQRSGANKVKKNPLKVTYISSPMKVKASNASEFRAIVQELTGKNS 60

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
KAG6599333.14.41e-3160.19hypothetical protein SDJN03_09111, partial [Cucurbita argyrosperma subsp. sorori... [more]
KGN60779.19.04e-2354.62hypothetical protein Csa_019238 [Cucumis sativus][more]
KAF3969566.11.46e-1162.50hypothetical protein CMV_006646 [Castanea mollissima][more]
XP_021282108.11.48e-1163.64uncharacterized protein LOC110414990 [Herrania umbratica][more]
XP_011468362.15.96e-1157.38PREDICTED: uncharacterized protein LOC105352572 [Fragaria vesca subsp. vesca][more]
Match NameE-valueIdentityDescription
A0A0A0LIA74.38e-2354.62VQ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G010150 PE=4 SV=... [more]
A0A6J1A5337.16e-1263.64uncharacterized protein LOC110414990 OS=Herrania umbratica OX=108875 GN=LOC11041... [more]
A0A5D2MMS71.00e-1052.94VQ domain-containing protein OS=Gossypium tomentosum OX=34277 GN=ES332_A13G20460... [more]
A0A067DJ561.21e-1057.38VQ domain-containing protein OS=Citrus sinensis OX=2711 GN=CISIN_1g031928mg PE=4... [more]
A0A2H5QH961.21e-1057.38VQ domain-containing protein OS=Citrus unshiu OX=55188 GN=CUMW_230020 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008889VQPFAMPF05678VQcoord: 32..59
e-value: 4.5E-9
score: 35.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..23
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 8..23
NoneNo IPR availablePANTHERPTHR33624:SF17VQ MOTIF PROTEINcoord: 8..61
IPR039335Sigma factor binding protein 1/2PANTHERPTHR33624SIGMA FACTOR BINDING PROTEIN 1, CHLOROPLASTICcoord: 8..61

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g2354.1MC08g2354.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051091 positive regulation of DNA-binding transcription factor activity