MC08g2279 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g2279 vs. ExPASy Swiss-Prot
Match: A0A1D6F9Y9 (Trimethyltridecatetraene synthase OS=Zea mays OX=4577 GN=CYP92C6 PE=1 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 1.2e-142
Identity = 256/507 (50.49%), Postives = 349/507 (68.84%), Query Frame = 0

Query: 26  GRRRKLNLPPGPKPWPFIGNLN-LIGPL-PHHSIHQLSKKHGPIMHLRFGSFPVVVASSI 85
           GR + LNLPPGP+ WP IG+L  L G L PH ++  L+ +HGP+MHLR GS+  VVASS 
Sbjct: 25  GRSKPLNLPPGPRGWPVIGSLGALAGALPPHRALAALAARHGPLMHLRLGSYHAVVASSA 84

Query: 86  ETAKLFLKTHDLNFISRPRLAAGKYIGYDYSDILWCPYGPYWRQAQKICVTELFCAKRLD 145
           +TA+L LK HDL F  RPR AAG+   Y Y  I+  PYG YWR A+K+C TELF A+R+D
Sbjct: 85  DTARLVLKVHDLAFADRPRTAAGEVASYGYLGIVHTPYGAYWRMARKLCATELFSARRVD 144

Query: 146 SLEYIRKEETNALLKEIYKFSGKG-IQLKYYLFTQSFNVISRMVLGKKYLEQSKEYSIFS 205
           S E +R +E  AL + +++ +G+G + ++ ++   +   I RM +G+K+   S  Y    
Sbjct: 145 SFERVRAQEMRALARGLFECAGRGAVAVREHVAGATLRNILRMAVGEKW---SGCYGSPE 204

Query: 206 PDEFRKMLEELFFLHGAL-NIGDFIPWIDFLDLQGYVKRMKALYKKFDRFLEHVLDEHNG 265
            + FR+ L+E F + GA+ N+G+++PW+ +LDLQG V+RMK L  ++DRF E +LD+H+ 
Sbjct: 205 GEAFRRTLDEAFAVTGAVSNVGEWVPWLGWLDLQGCVRRMKRLRAQYDRFFEQILDDHDH 264

Query: 266 R-------RMGVRDNYVAKDMVDVLLQLA--DDPNLEV----KLERQGVKAITQDLIAAG 325
           +       R G  D+    D+VDVLL+L   D+   E     +L R GVKA  QD++A G
Sbjct: 265 KKTRRERGRPGAGDSAADDDLVDVLLRLVEEDEDRPETTEASRLTRDGVKAFVQDIVAGG 324

Query: 326 TESSVVTIEWALSELLKRPDIFKKTTSELDKVVGRERWVEEKDIANLPYINAIVKETMRL 385
           TESS VTIEWA+SELL+RPD  +  T+ELD+V+G  RWV E+D+ +LPYI+A+VKETMRL
Sbjct: 325 TESSAVTIEWAMSELLRRPDALRAATAELDRVIGHGRWVTERDLPDLPYIDAVVKETMRL 384

Query: 386 HPIAPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGRDPAIW-ENPNDFIPERF----S 445
           HP+ P+LVP  +RE   +AGYD+  G RVLVNVW I RDPA W + P+ F PERF    S
Sbjct: 385 HPVGPLLVPHHAREHTVVAGYDVPAGARVLVNVWAIARDPASWPDAPDAFRPERFLNGSS 444

Query: 446 GKSIDVKGQDFELLPLGSGRRMCPGYNLGLKVVQSSLANLLHGFVWKLEGDTKKEDLNMD 505
           G S+DV+G  FELLP G+GRRMCP + L +K+V + +ANL+HGF W+L      ED++M+
Sbjct: 445 GASVDVRGAHFELLPFGAGRRMCPAHGLAMKLVTAGVANLVHGFAWRLPDGMAPEDVSME 504

Query: 506 ETFGLSTPKKFPLVVIAEPRLPPHLYS 511
           E FGLST +K PLV +AEPRLP HLY+
Sbjct: 505 ELFGLSTRRKVPLVAVAEPRLPAHLYT 528

BLAST of MC08g2279 vs. ExPASy Swiss-Prot
Match: A0A1D6HSP4 (Dimethylnonatriene synthase OS=Zea mays OX=4577 GN=CYP92C5 PE=1 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 4.0e-130
Identity = 237/517 (45.84%), Postives = 338/517 (65.38%), Query Frame = 0

Query: 10  AALWVAIATLSLLLLSGRRRKLNLPPGPKPWPFIGNLN-LIGPL-PHHSIHQLSKKHGPI 69
           AA++V + ++       R + L LPPGP+ WP +G+L  L G L PH ++  L+ +HGP+
Sbjct: 15  AAIFVVLCSVVASARGRREKALKLPPGPRGWPVLGSLGALAGALPPHRALAALAARHGPL 74

Query: 70  MHLRFGSFPVVVASSIETAKLFLKTHDLNFISRPRLAAGKYIGYDYSDILWCPYGPYWRQ 129
           MHLR GS+  VVASS + A+L L+THD     RP  AAG+   Y Y  I+  P G YWR 
Sbjct: 75  MHLRLGSYHTVVASSADAARLVLRTHDSALADRPDTAAGEITSYGYLGIVHTPRGAYWRM 134

Query: 130 AQKICVTELFCAKRLDSLEYIRKEETNALLKEIYKFSG--KGIQLKYYLFTQSFNVISRM 189
           A+++C TELF A+R++S + +R +E  AL + ++  +   + + ++ ++   +   I RM
Sbjct: 135 ARRLCATELFSARRVESFQDVRAQEMRALARGLFGCAAGRRAVAVREHVAGATMRNILRM 194

Query: 190 VLGKKYLEQSKEYSIFSPDEFRKMLEELFFLHGAL-NIGDFIPWIDFLDLQGYVKRMKAL 249
            +G+K+   S  Y     + FR+ L+E F   GA+ N+G+++PW+ +LD+QG+ ++MK L
Sbjct: 195 AVGEKW---SGCYGSPEGEAFRRSLDEAFAATGAVSNVGEWVPWLGWLDVQGFKRKMKRL 254

Query: 250 YKKFDRFLEHVLDEHNGRR---MGVRDNYVAKDMVDVLLQLAD-----DPNLEVKLERQG 309
           +   D F E +L +H  RR         +VA D+VDVLLQL++     +   E +L R G
Sbjct: 255 HDLHDHFYEKILVDHEERRRLAQASGGEFVATDLVDVLLQLSEESTKLESESEARLPRDG 314

Query: 310 VKAITQDLIAAGTESSVVTIEWALSELLKRPDIFKKTTSELDKVVGRERWVEEKDIANLP 369
           VKA+ QD+IA GTESS VTIEWA++ELL+ P+   K T ELD+VVG  RWV E+D+  L 
Sbjct: 315 VKALIQDIIAGGTESSAVTIEWAMAELLRHPEAMAKATDELDRVVGSGRWVAERDLPELH 374

Query: 370 YINAIVKETMRLHPIAPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGRDPAIW-ENPN 429
           YI+A+VKET+RLHP+ P+LVP  +RE   +AGYD+  G RVLVN W I RDPA W + P+
Sbjct: 375 YIDAVVKETLRLHPVGPLLVPHYARERTVVAGYDVPAGARVLVNAWAIARDPASWPDAPD 434

Query: 430 DFIPERFSG--KSIDVKGQDFELLPLGSGRRMCPGYNLGLKVVQSSLANLLHGFVWKLEG 489
            F PERF G   ++DV+G  FELLP GSGRR+CP Y+L +K+V + +ANL+HGF W+L  
Sbjct: 435 AFQPERFLGAAAAVDVRGAHFELLPFGSGRRICPAYDLAMKLVAAGVANLVHGFAWRLPD 494

Query: 490 DTKKEDLNMDETFGLSTPKKFPLVVIAEPRLPPHLYS 511
               ED++M+E  GLST +K PL  +AEPRLP HLYS
Sbjct: 495 GVAAEDVSMEEHVGLSTRRKVPLFAVAEPRLPVHLYS 528

BLAST of MC08g2279 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 428.7 bits (1101), Expect = 9.2e-119
Identity = 222/501 (44.31%), Postives = 314/501 (62.67%), Query Frame = 0

Query: 12  LWVAIA-TLSLLLLSGRR-RKLNLPPGPKPWPFIGNLNLIGPLPHHSIHQLSKKHGPIMH 71
           L++AIA T  LL L+ +R +K NLPP P   P IGNL+ +G LPH S+  L+ + GP++ 
Sbjct: 8   LFLAIALTFFLLKLNEKREKKPNLPPSPPNLPIIGNLHQLGNLPHRSLRSLANELGPLIL 67

Query: 72  LRFGSFPVVVASSIETAKLFLKTHDLNFISRPRLAAGKYIGYDYSDILWCPYGPYWRQAQ 131
           L  G  P ++ S+ E A+  LKTHDL F SRP   A + I YD +D+ + PYG YWRQ +
Sbjct: 68  LHLGHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGEYWRQVR 127

Query: 132 KICVTELFCAKRLDSLEYIRKEETNALLKEIYK--FSGKGIQLKYYLFTQSFNVISRMVL 191
           KICV EL   KR++S   IR+EE   +++ I +   +G+ + L   L   S   I+R+  
Sbjct: 128 KICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSGTITRVAF 187

Query: 192 GKKYLEQSKEYSIFSPDEFRKMLEELFFLHGALNIGDFIP---WIDFLDLQGYVKRMKAL 251
           GKKY  + +       ++F  +  EL  L GA  +GD+ P   W+D   L G   R+K  
Sbjct: 188 GKKYEGEEER-----KNKFADLATELTTLMGAFFVGDYFPSFAWVDV--LTGMDARLKRN 247

Query: 252 YKKFDRFLEHVLDEH-NGRRMGVRDNYVAKDMVDVLLQLADDPNLEVKLERQGVKAITQD 311
           + + D F++HV+D+H   R+    D    KD+VDVLL L  D +L V L R  +KA+  D
Sbjct: 248 HGELDAFVDHVIDDHLLSRKANGSDGVEQKDLVDVLLHLQKDSSLGVHLNRNNLKAVILD 307

Query: 312 LIAAGTESSVVTIEWALSELLKRPDIFKKTTSELDKVVGRERWVEEKDIANLPYINAIVK 371
           + + GT+++ VT+EWA++EL+K PD+ +K   E+ +VVG++  VEE+D+  L Y+  I+K
Sbjct: 308 MFSGGTDTTAVTLEWAMAELIKHPDVMEKAQQEVRRVVGKKAKVEEEDLHQLHYLKLIIK 367

Query: 372 ETMRLHPIAPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGRDPAIWENPNDFIPERFS 431
           ET+RLHP+AP+LVPR S  D  I GY I   TRV +N W IGRDP  WEN  +F+PERF 
Sbjct: 368 ETLRLHPVAPLLVPRESTRDVVIRGYHIPAKTRVFINAWAIGRDPKSWENAEEFLPERFV 427

Query: 432 GKSIDVKGQDFELLPLGSGRRMCPGYNLGLKVVQSSLANLLHGFVWKLEGDTKKEDLNMD 491
             S+D KGQDF+L+P G+GRR CPG   G+  V+ SLANLL+ F W+L GD  KEDL+M 
Sbjct: 428 NNSVDFKGQDFQLIPFGAGRRGCPGIAFGISSVEISLANLLYWFNWELPGDLTKEDLDMS 487

Query: 492 ETFGLSTPKKFPLVVIAEPRL 505
           E  G++   KFPL ++A+  L
Sbjct: 488 EAVGITVHMKFPLQLVAKRHL 501

BLAST of MC08g2279 vs. ExPASy Swiss-Prot
Match: Q9SBQ9 (Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 7.8e-118
Identity = 210/489 (42.94%), Postives = 301/489 (61.55%), Query Frame = 0

Query: 27  RRRKLNLPPGPKPWPFIGNLNLIGPLPHHSIHQLSKKHGPIMHLRFGSFPVVVASSIETA 86
           +R  L LPPGPKPWP IGNL  +GP PH S   +++ +GP+M+L+ G   VVVA+S   A
Sbjct: 27  KRYPLPLPPGPKPWPIIGNLVHLGPKPHQSTAAMAQTYGPLMYLKMGFVDVVVAASASVA 86

Query: 87  KLFLKTHDLNFISRPRLAAGKYIGYDYSDILWCPYGPYWRQAQKICVTELFCAKRLDSLE 146
             FLKTHD NF SRP  +  +++ Y+Y D+++ PYGP WR  +KIC   LF  K LD   
Sbjct: 87  AQFLKTHDANFSSRPPNSGAEHMAYNYQDLVFAPYGPRWRMLRKICSVHLFSTKALDDFR 146

Query: 147 YIRKEETNALLKEIYKFSGKGIQLKYYLFTQSFNVISRMVLGKKYLEQSKEYSIFSPDEF 206
           ++R++E   L + +     K ++L   L   + N ++R++LGK+              EF
Sbjct: 147 HVRQDEVKTLTRALASAGQKPVKLGQLLNVCTTNALARVMLGKRVFADGSGDVDPQAAEF 206

Query: 207 RKMLEELFFLHGALNIGDFIPWIDFLDLQGYVKRMKALYKKFDRFLEHVLDEHNGRRMGV 266
           + M+ E+  + G  NIGDFIP +++LD+QG   +MK L+ +FD FL  +L+EH G+  G 
Sbjct: 207 KSMVVEMMVVAGVFNIGDFIPQLNWLDIQGVAAKMKKLHARFDAFLTDILEEHKGKIFG- 266

Query: 267 RDNYVAKDMVDVLLQLADD--PNLEVKLERQGVKAITQDLIAAGTESSVVTIEWALSELL 326
                 KD++  L+ L +D   N   KL    +KA+  +L  AGT++S  T+EWA++EL+
Sbjct: 267 ----EMKDLLSTLISLKNDDADNDGGKLTDTEIKALLLNLFVAGTDTSSSTVEWAIAELI 326

Query: 327 KRPDIFKKTTSELDKVVGRERWVEEKDIANLPYINAIVKETMRLHPIAPMLVPRLSREDC 386
           + P I  +   E+DKVVGR+R V E D+A L Y+ AIVKET RLHP  P+ +PR++ E C
Sbjct: 327 RNPKILAQAQQEIDKVVGRDRLVGELDLAQLTYLEAIVKETFRLHPSTPLSLPRIASESC 386

Query: 387 QIAGYDIAKGTRVLVNVWTIGRDPAIWENPNDFIPERF----SGKSIDVKGQDFELLPLG 446
           +I GY I KG+ +L+NVW I RDP  W +P +F PERF        +DV+G DFE++P G
Sbjct: 387 EINGYFIPKGSTLLLNVWAIARDPNAWADPLEFRPERFLPGGEKPKVDVRGNDFEVIPFG 446

Query: 447 SGRRMCPGYNLGLKVVQSSLANLLHGFVWKLEGDTKKEDLNMDETFGLSTPKKFPLVVIA 506
           +GRR+C G NLG+++VQ  +A L+H F W L      E LNM+E +GL+  +  PLVV  
Sbjct: 447 AGRRICAGMNLGIRMVQLMIATLIHAFNWDLVSGQLPEMLNMEEAYGLTLQRADPLVVHP 506

Query: 507 EPRLPPHLY 510
            PRL    Y
Sbjct: 507 RPRLEAQAY 510

BLAST of MC08g2279 vs. ExPASy Swiss-Prot
Match: A0A4D6Q415 (Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B137 PE=1 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 1.5e-116
Identity = 210/510 (41.18%), Postives = 320/510 (62.75%), Query Frame = 0

Query: 12  LWVAIATLS--LLLLSGRRRKLN---LPPGPKPWPFIGNLNLIGPLPHHSIHQLSKKHGP 71
           LW++   LS  ++ L  RRR      LPPGP  WP +GNL  +G  PH ++  LSK++GP
Sbjct: 7   LWISTLLLSSFIVYLLYRRRSAQCPPLPPGPNGWPILGNLPQLGAKPHQTLDALSKQYGP 66

Query: 72  IMHLRFGSFPVVVASSIETAKLFLKTHDLNFISRPRLAAGKYIGYDYSDILWCPYGPYWR 131
           +  LR GS  VVVASS   A  FL+THD+NF +RP  +  +++ Y+Y D+++ PYGP WR
Sbjct: 67  LFRLRLGSVNVVVASSSAVAAQFLRTHDVNFSNRPPNSGAEHVAYNYQDLVFAPYGPRWR 126

Query: 132 QAQKICVTELFCAKRLDSLEYIRKEETNALLKEIYKFSGKG--IQLKYYLFTQSFNVISR 191
             +K+C   LF  K LD L  +R+ E   L++ + + +  G  + L   L   + N ++R
Sbjct: 127 MLRKLCSVHLFSLKALDDLRPVRQGEVACLVRNLRRHADTGVLVNLGKALNVCATNALAR 186

Query: 192 MVLGKKYLEQSKEYSIFSPDEFRKMLEELFFLHGALNIGDFIPWIDFLDLQGYVKRMKAL 251
            +LG++     ++  +   DEF++M+ EL  L G  N+GDF+P + +LDLQG V +MK L
Sbjct: 187 AMLGRRVF-ADEDAQLAEADEFKEMVVELMRLAGVFNVGDFVPGLGWLDLQGVVGKMKRL 246

Query: 252 YKKFDRFLEHVLDEHNGRRMGVRDNYVAKDMVDVLLQLAD-DPNLEVKLERQGVKAITQD 311
           ++++D FL+ V++E+         N  + D++ VL++L + D   E+KL    +KA+  +
Sbjct: 247 HRRYDAFLDRVIEENQA-------NAKSGDLLSVLIRLKEADAEGEIKLNNTDIKALLLN 306

Query: 312 LIAAGTESSVVTIEWALSELLKRPDIFKKTTSELDKVVGRERWVEEKDIANLPYINAIVK 371
           L  AGT++S  T+EW L+EL++ PDI +KT  ELD V+GR+R V E D+ NLPY+ A+VK
Sbjct: 307 LFTAGTDTSSSTVEWVLAELIRHPDILQKTQHELDSVIGRDRLVAESDLPNLPYLQAVVK 366

Query: 372 ETMRLHPIAPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGRDPAIWENPNDFIPERF- 431
           ET RLHP  P+ +PR++ E+C + GY I K   +LVNVW+IGRD A+W +P +F P RF 
Sbjct: 367 ETFRLHPSTPLSLPRMASEECIVNGYKIPKHATLLVNVWSIGRDAAVWNDPLEFRPSRFL 426

Query: 432 ---SGKSIDVKGQDFELLPLGSGRRMCPGYNLGLKVVQSSLANLLHGFVWKLEGDTKKED 491
                + +DVKG DFE++P G+GRR+C G +LGL++VQ   A ++H + W L    + + 
Sbjct: 427 PGGEREHVDVKGNDFEVIPFGAGRRICAGLSLGLRMVQFMTATIVHAYDWSLPKGQECQK 486

Query: 492 LNMDETFGLSTPKKFPLVVIAEPRLPPHLY 510
           L+M+E +GL+  +  PL+V   PRL    Y
Sbjct: 487 LDMEEAYGLTLQRAVPLMVQPIPRLSHKAY 508

BLAST of MC08g2279 vs. NCBI nr
Match: XP_022150738.1 (cytochrome P450 71A1-like [Momordica charantia])

HSP 1 Score: 1045 bits (2702), Expect = 0.0
Identity = 511/511 (100.00%), Postives = 511/511 (100.00%), Query Frame = 0

Query: 1   MEAPCWLSHAALWVAIATLSLLLLSGRRRKLNLPPGPKPWPFIGNLNLIGPLPHHSIHQL 60
           MEAPCWLSHAALWVAIATLSLLLLSGRRRKLNLPPGPKPWPFIGNLNLIGPLPHHSIHQL
Sbjct: 1   MEAPCWLSHAALWVAIATLSLLLLSGRRRKLNLPPGPKPWPFIGNLNLIGPLPHHSIHQL 60

Query: 61  SKKHGPIMHLRFGSFPVVVASSIETAKLFLKTHDLNFISRPRLAAGKYIGYDYSDILWCP 120
           SKKHGPIMHLRFGSFPVVVASSIETAKLFLKTHDLNFISRPRLAAGKYIGYDYSDILWCP
Sbjct: 61  SKKHGPIMHLRFGSFPVVVASSIETAKLFLKTHDLNFISRPRLAAGKYIGYDYSDILWCP 120

Query: 121 YGPYWRQAQKICVTELFCAKRLDSLEYIRKEETNALLKEIYKFSGKGIQLKYYLFTQSFN 180
           YGPYWRQAQKICVTELFCAKRLDSLEYIRKEETNALLKEIYKFSGKGIQLKYYLFTQSFN
Sbjct: 121 YGPYWRQAQKICVTELFCAKRLDSLEYIRKEETNALLKEIYKFSGKGIQLKYYLFTQSFN 180

Query: 181 VISRMVLGKKYLEQSKEYSIFSPDEFRKMLEELFFLHGALNIGDFIPWIDFLDLQGYVKR 240
           VISRMVLGKKYLEQSKEYSIFSPDEFRKMLEELFFLHGALNIGDFIPWIDFLDLQGYVKR
Sbjct: 181 VISRMVLGKKYLEQSKEYSIFSPDEFRKMLEELFFLHGALNIGDFIPWIDFLDLQGYVKR 240

Query: 241 MKALYKKFDRFLEHVLDEHNGRRMGVRDNYVAKDMVDVLLQLADDPNLEVKLERQGVKAI 300
           MKALYKKFDRFLEHVLDEHNGRRMGVRDNYVAKDMVDVLLQLADDPNLEVKLERQGVKAI
Sbjct: 241 MKALYKKFDRFLEHVLDEHNGRRMGVRDNYVAKDMVDVLLQLADDPNLEVKLERQGVKAI 300

Query: 301 TQDLIAAGTESSVVTIEWALSELLKRPDIFKKTTSELDKVVGRERWVEEKDIANLPYINA 360
           TQDLIAAGTESSVVTIEWALSELLKRPDIFKKTTSELDKVVGRERWVEEKDIANLPYINA
Sbjct: 301 TQDLIAAGTESSVVTIEWALSELLKRPDIFKKTTSELDKVVGRERWVEEKDIANLPYINA 360

Query: 361 IVKETMRLHPIAPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGRDPAIWENPNDFIPE 420
           IVKETMRLHPIAPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGRDPAIWENPNDFIPE
Sbjct: 361 IVKETMRLHPIAPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGRDPAIWENPNDFIPE 420

Query: 421 RFSGKSIDVKGQDFELLPLGSGRRMCPGYNLGLKVVQSSLANLLHGFVWKLEGDTKKEDL 480
           RFSGKSIDVKGQDFELLPLGSGRRMCPGYNLGLKVVQSSLANLLHGFVWKLEGDTKKEDL
Sbjct: 421 RFSGKSIDVKGQDFELLPLGSGRRMCPGYNLGLKVVQSSLANLLHGFVWKLEGDTKKEDL 480

Query: 481 NMDETFGLSTPKKFPLVVIAEPRLPPHLYSL 511
           NMDETFGLSTPKKFPLVVIAEPRLPPHLYSL
Sbjct: 481 NMDETFGLSTPKKFPLVVIAEPRLPPHLYSL 511

BLAST of MC08g2279 vs. NCBI nr
Match: XP_022152553.1 (cytochrome P450 71A1-like [Momordica charantia])

HSP 1 Score: 825 bits (2132), Expect = 1.83e-298
Identity = 398/511 (77.89%), Postives = 446/511 (87.28%), Query Frame = 0

Query: 1   MEAPCWLSHAALWVAIATLSLLLLSGRRRKLNLPPGPKPWPFIGNLNLIGPLPHHSIHQL 60
           ME P W+S+AA WVA   L LL    RRRKLNLPPGPKPWPFIGNLNLIG LPH SIHQL
Sbjct: 1   MELPSWVSYAAAWVATVALLLLSRRLRRRKLNLPPGPKPWPFIGNLNLIGSLPHQSIHQL 60

Query: 61  SKKHGPIMHLRFGSFPVVVASSIETAKLFLKTHDLNFISRPRLAAGKYIGYDYSDILWCP 120
           S+K+G IMHLRFGSFPVVV SS+E AK+FLKTHDL F+SRP+ AAGKY  Y+YSDI W  
Sbjct: 61  SRKYGSIMHLRFGSFPVVVGSSVEMAKIFLKTHDLTFVSRPKTAAGKYTTYNYSDITWSQ 120

Query: 121 YGPYWRQAQKICVTELFCAKRLDSLEYIRKEETNALLKEIYKFSGKGIQLKYYLFTQSFN 180
           YGPYWRQA+K+C+ ELF AKRLDS EYIR+EE +ALL++IY+  G+ IQ+K YL T S N
Sbjct: 121 YGPYWRQARKMCLMELFSAKRLDSYEYIRREEMSALLRDIYRSCGRAIQVKDYLSTVSLN 180

Query: 181 VISRMVLGKKYLEQSKEYSIFSPDEFRKMLEELFFLHGALNIGDFIPWIDFLDLQGYVKR 240
           VISRMVLGKKY ++S E +I SPDEF+KML+ELF L G LNIGD IPWIDFLDLQGY+KR
Sbjct: 181 VISRMVLGKKYTDES-ENAIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYIKR 240

Query: 241 MKALYKKFDRFLEHVLDEHNGRRMGVRDNYVAKDMVDVLLQLADDPNLEVKLERQGVKAI 300
           MKAL KKFDRFLEHVLDEHN RR G++D YVAKDMVDVLLQLADDP+LEVKLER GVKA 
Sbjct: 241 MKALSKKFDRFLEHVLDEHNERRKGIKD-YVAKDMVDVLLQLADDPDLEVKLERHGVKAF 300

Query: 301 TQDLIAAGTESSVVTIEWALSELLKRPDIFKKTTSELDKVVGRERWVEEKDIANLPYINA 360
           TQDLIA GTESS VT+EWA+SELLK+P+IF K T ELD+V+G+ERWVEEKDI NLPYI+A
Sbjct: 301 TQDLIAGGTESSAVTVEWAMSELLKKPEIFIKATEELDRVIGKERWVEEKDITNLPYIDA 360

Query: 361 IVKETMRLHPIAPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGRDPAIWENPNDFIPE 420
           I KETMRLHP+APMLVPR+SREDC+IAGYDIAK TR+LVNVWTIGRDP +WENPN+F PE
Sbjct: 361 IAKETMRLHPVAPMLVPRMSREDCKIAGYDIAKDTRILVNVWTIGRDPEVWENPNEFNPE 420

Query: 421 RFSGKSIDVKGQDFELLPLGSGRRMCPGYNLGLKVVQSSLANLLHGFVWKLEGDTKKEDL 480
           RF GKSIDVKGQDFELLP GSGRRMCPGY+LGLKV+QSSLANLLHGF WKL GD +KEDL
Sbjct: 421 RFIGKSIDVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFSWKLGGDMRKEDL 480

Query: 481 NMDETFGLSTPKKFPLVVIAEPRLPPHLYSL 511
           NM+E FGLSTPKKFPLV +AEPRLPPHLYS+
Sbjct: 481 NMEEIFGLSTPKKFPLVAVAEPRLPPHLYSM 509

BLAST of MC08g2279 vs. NCBI nr
Match: XP_038877760.1 (trimethyltridecatetraene synthase-like [Benincasa hispida])

HSP 1 Score: 820 bits (2119), Expect = 1.87e-296
Identity = 404/513 (78.75%), Postives = 446/513 (86.94%), Query Frame = 0

Query: 1   MEAPCWLSHAALWVAIATLSLLLLSGR--RRKLNLPPGPKPWPFIGNLNLIGPLPHHSIH 60
           M+ P W+S+AA W+A  TL+LLLLS R  RRKLNLPPGP+PWP IGNLNLIG LPH SIH
Sbjct: 3   MQLPSWVSYAAAWMA--TLALLLLSRRLCRRKLNLPPGPRPWPLIGNLNLIGSLPHQSIH 62

Query: 61  QLSKKHGPIMHLRFGSFPVVVASSIETAKLFLKTHDLNFISRPRLAAGKYIGYDYSDILW 120
           QLSKK+GPIMHLRFGSFPVVV SS+E AK+FLKTHDLNF+SRP+ AAGKY  Y+YSDI W
Sbjct: 63  QLSKKYGPIMHLRFGSFPVVVGSSVEMAKIFLKTHDLNFVSRPKTAAGKYTTYNYSDITW 122

Query: 121 CPYGPYWRQAQKICVTELFCAKRLDSLEYIRKEETNALLKEIYKFSGKGIQLKYYLFTQS 180
             YGPYWRQA+K+C+ ELF A+RLDS EYIRKEE NALLK+IYK  G+ I+LK YL T S
Sbjct: 123 SQYGPYWRQARKMCLMELFSARRLDSYEYIRKEEMNALLKDIYKSRGQVIKLKDYLSTVS 182

Query: 181 FNVISRMVLGKKYLEQSKEYSIFSPDEFRKMLEELFFLHGALNIGDFIPWIDFLDLQGYV 240
            NVISRMVLG+KY ++S E +I SPDEF+KML+ELF L G LNIGD IPWIDFLDLQGYV
Sbjct: 183 LNVISRMVLGRKYTDES-ENAIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYV 242

Query: 241 KRMKALYKKFDRFLEHVLDEHNGRRMGVRDNYVAKDMVDVLLQLADDPNLEVKLERQGVK 300
           KRMKAL KKFDRFLEHVLDEHN RR GV +NYVAKDMVDVLLQ ADDPNLEVKLER GVK
Sbjct: 243 KRMKALSKKFDRFLEHVLDEHNERRKGV-ENYVAKDMVDVLLQQADDPNLEVKLERHGVK 302

Query: 301 AITQDLIAAGTESSVVTIEWALSELLKRPDIFKKTTSELDKVVGRERWVEEKDIANLPYI 360
           A TQDLIA GTESS VTIEWA+SELLK+P+IF K T ELD+V+GRERWVEEKDI NLPYI
Sbjct: 303 AFTQDLIAGGTESSAVTIEWAISELLKQPEIFDKATEELDRVIGRERWVEEKDIVNLPYI 362

Query: 361 NAIVKETMRLHPIAPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGRDPAIWENPNDFI 420
           NAI KETMRLHP+APMLVPR+ REDCQIAGYDIAKGTRVLVNVWTIGRDP +W+NP  F 
Sbjct: 363 NAIAKETMRLHPVAPMLVPRMCREDCQIAGYDIAKGTRVLVNVWTIGRDPTVWKNPYAFD 422

Query: 421 PERFSGKSIDVKGQDFELLPLGSGRRMCPGYNLGLKVVQSSLANLLHGFVWKLEGDTKKE 480
           PERF   SIDVKGQDFELLP GSGRRMCPGY+LGLKV+QSSLANLLHGF WKL GD +KE
Sbjct: 423 PERFMENSIDVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFSWKLPGDMEKE 482

Query: 481 DLNMDETFGLSTPKKFPLVVIAEPRLPPHLYSL 511
           DLN++E FGLSTPKKFPL  +A+PRLPPHLYSL
Sbjct: 483 DLNLEEIFGLSTPKKFPLDAVADPRLPPHLYSL 511

BLAST of MC08g2279 vs. NCBI nr
Match: XP_023526994.1 (cytochrome P450 71A1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 816 bits (2109), Expect = 6.73e-295
Identity = 393/508 (77.36%), Postives = 442/508 (87.01%), Query Frame = 0

Query: 4   PCWLSHAALWVAIATLSLLLLSGRRRKLNLPPGPKPWPFIGNLNLIGPLPHHSIHQLSKK 63
           P W+S+AA W+AI  L  L    RRR LNLPPGPKPWP IGNL+LIGPLPH SIH LSKK
Sbjct: 8   PSWVSYAAAWLAILALFFLSRRLRRRNLNLPPGPKPWPLIGNLDLIGPLPHQSIHHLSKK 67

Query: 64  HGPIMHLRFGSFPVVVASSIETAKLFLKTHDLNFISRPRLAAGKYIGYDYSDILWCPYGP 123
           +GPIMHLRFGSFPVVV SS+E AK+FLKTHDLNF+SRP+ AAGKY  Y+YSDI W  YGP
Sbjct: 68  YGPIMHLRFGSFPVVVGSSVEMAKIFLKTHDLNFVSRPKTAAGKYTTYNYSDITWSQYGP 127

Query: 124 YWRQAQKICVTELFCAKRLDSLEYIRKEETNALLKEIYKFSGKGIQLKYYLFTQSFNVIS 183
           YWRQA+K+C+ ELF AKRLDS EYIR+EE NALL+++YK  G  I++K YL T S NVIS
Sbjct: 128 YWRQARKMCLMELFSAKRLDSYEYIRREEMNALLRDVYKSCGDVIKVKDYLSTVSLNVIS 187

Query: 184 RMVLGKKYLEQSKEYSIFSPDEFRKMLEELFFLHGALNIGDFIPWIDFLDLQGYVKRMKA 243
           RMVLGKKY ++S E  I SPDEF+KML+ELF L G LNIGD IPWIDFLDLQGYVKRMKA
Sbjct: 188 RMVLGKKYTDES-ENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMKA 247

Query: 244 LYKKFDRFLEHVLDEHNGRRMGVRDNYVAKDMVDVLLQLADDPNLEVKLERQGVKAITQD 303
           L KKFDRFLEHVLDEHN RR GV+D YVA+DMVDVLLQLADDP+LEVKLER GVKA TQD
Sbjct: 248 LSKKFDRFLEHVLDEHNARREGVKD-YVAQDMVDVLLQLADDPDLEVKLERHGVKAFTQD 307

Query: 304 LIAAGTESSVVTIEWALSELLKRPDIFKKTTSELDKVVGRERWVEEKDIANLPYINAIVK 363
           LIA GTESS VT+EWA+SE+L++P+I KK   ELD+V+G+ERWVEEKDIA+LPY++AI K
Sbjct: 308 LIAGGTESSAVTVEWAMSEVLRKPEILKKAREELDRVIGKERWVEEKDIASLPYMDAIAK 367

Query: 364 ETMRLHPIAPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGRDPAIWENPNDFIPERFS 423
           ETMRLHP+APMLVPR+ REDC+IAGYDIAKGTRVLVNVWTIGRDPA+WENPN+F PERF 
Sbjct: 368 ETMRLHPVAPMLVPRMCREDCEIAGYDIAKGTRVLVNVWTIGRDPAVWENPNEFSPERFM 427

Query: 424 GKSIDVKGQDFELLPLGSGRRMCPGYNLGLKVVQSSLANLLHGFVWKLEGDTKKEDLNMD 483
           GK+IDVKGQDFELLP GSGRRMCPGY+LGLKV+QSSLANLLHGF W L GD KKEDLNMD
Sbjct: 428 GKTIDVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFSWSLGGDMKKEDLNMD 487

Query: 484 ETFGLSTPKKFPLVVIAEPRLPPHLYSL 511
           E FGLSTPKKFPL V+A+PRLPPHLYS+
Sbjct: 488 EVFGLSTPKKFPLHVVAQPRLPPHLYSI 513

BLAST of MC08g2279 vs. NCBI nr
Match: KAG7018217.1 (Flavonoid 3'-monooxygenase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 816 bits (2107), Expect = 1.36e-294
Identity = 395/508 (77.76%), Postives = 439/508 (86.42%), Query Frame = 0

Query: 4   PCWLSHAALWVAIATLSLLLLSGRRRKLNLPPGPKPWPFIGNLNLIGPLPHHSIHQLSKK 63
           P W+S++A W AI  L  L    RRR LNLPPGPKPWP IGNL+LIGPLPH SIH LSKK
Sbjct: 8   PSWVSYSAAWFAILALFFLSRRLRRRNLNLPPGPKPWPLIGNLDLIGPLPHQSIHHLSKK 67

Query: 64  HGPIMHLRFGSFPVVVASSIETAKLFLKTHDLNFISRPRLAAGKYIGYDYSDILWCPYGP 123
           +GPIMHLRFGSFPVVV SS+E AK+FLKTHDLNF+SRP+ AAGKY  Y+YSDI W  YGP
Sbjct: 68  YGPIMHLRFGSFPVVVGSSVEMAKIFLKTHDLNFVSRPKTAAGKYTTYNYSDITWSQYGP 127

Query: 124 YWRQAQKICVTELFCAKRLDSLEYIRKEETNALLKEIYKFSGKGIQLKYYLFTQSFNVIS 183
           YWRQA+K+C+ ELF AKRLDS EYIR+EE NALL+++YK  G  I++K YL T S NVIS
Sbjct: 128 YWRQARKMCLMELFSAKRLDSYEYIRREEMNALLRDLYKSCGDVIKVKDYLSTVSLNVIS 187

Query: 184 RMVLGKKYLEQSKEYSIFSPDEFRKMLEELFFLHGALNIGDFIPWIDFLDLQGYVKRMKA 243
           RMVLGKKY ++S E  I SPDEF+KML+ELF L G LNIGD IPWIDFLDLQGYVKRMKA
Sbjct: 188 RMVLGKKYTDES-ENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMKA 247

Query: 244 LYKKFDRFLEHVLDEHNGRRMGVRDNYVAKDMVDVLLQLADDPNLEVKLERQGVKAITQD 303
           L KKFDRFLEHVLDEHN RR GV+D YVA+DMVDVLLQLADDP+LEVKLER GVKA TQD
Sbjct: 248 LSKKFDRFLEHVLDEHNARREGVKD-YVAQDMVDVLLQLADDPDLEVKLERHGVKAFTQD 307

Query: 304 LIAAGTESSVVTIEWALSELLKRPDIFKKTTSELDKVVGRERWVEEKDIANLPYINAIVK 363
           LIA GTESS VT+EWA+SELL++P+I  K   ELD+V+G+ERWVEEKDIA+LPYI+AI K
Sbjct: 308 LIAGGTESSAVTVEWAMSELLRKPEILNKAREELDRVIGKERWVEEKDIASLPYIDAIAK 367

Query: 364 ETMRLHPIAPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGRDPAIWENPNDFIPERFS 423
           ETMRLHP+APMLVPR+ REDCQIAGYDIAKGTRVLVNVWTIGRDPA+WENPN+F PERF 
Sbjct: 368 ETMRLHPVAPMLVPRMCREDCQIAGYDIAKGTRVLVNVWTIGRDPAVWENPNEFSPERFM 427

Query: 424 GKSIDVKGQDFELLPLGSGRRMCPGYNLGLKVVQSSLANLLHGFVWKLEGDTKKEDLNMD 483
           GKSIDVKGQDFELLP GSGRRMCPGY+LGLKV+QSSLANLLHGF W L GD KKEDLNMD
Sbjct: 428 GKSIDVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFTWCLGGDMKKEDLNMD 487

Query: 484 ETFGLSTPKKFPLVVIAEPRLPPHLYSL 511
           E FGLSTPKKFPL V+A PRLPPHLYS+
Sbjct: 488 EVFGLSTPKKFPLDVVAHPRLPPHLYSI 513

BLAST of MC08g2279 vs. ExPASy TrEMBL
Match: A0A6J1DA87 (cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111018796 PE=3 SV=1)

HSP 1 Score: 1045 bits (2702), Expect = 0.0
Identity = 511/511 (100.00%), Postives = 511/511 (100.00%), Query Frame = 0

Query: 1   MEAPCWLSHAALWVAIATLSLLLLSGRRRKLNLPPGPKPWPFIGNLNLIGPLPHHSIHQL 60
           MEAPCWLSHAALWVAIATLSLLLLSGRRRKLNLPPGPKPWPFIGNLNLIGPLPHHSIHQL
Sbjct: 1   MEAPCWLSHAALWVAIATLSLLLLSGRRRKLNLPPGPKPWPFIGNLNLIGPLPHHSIHQL 60

Query: 61  SKKHGPIMHLRFGSFPVVVASSIETAKLFLKTHDLNFISRPRLAAGKYIGYDYSDILWCP 120
           SKKHGPIMHLRFGSFPVVVASSIETAKLFLKTHDLNFISRPRLAAGKYIGYDYSDILWCP
Sbjct: 61  SKKHGPIMHLRFGSFPVVVASSIETAKLFLKTHDLNFISRPRLAAGKYIGYDYSDILWCP 120

Query: 121 YGPYWRQAQKICVTELFCAKRLDSLEYIRKEETNALLKEIYKFSGKGIQLKYYLFTQSFN 180
           YGPYWRQAQKICVTELFCAKRLDSLEYIRKEETNALLKEIYKFSGKGIQLKYYLFTQSFN
Sbjct: 121 YGPYWRQAQKICVTELFCAKRLDSLEYIRKEETNALLKEIYKFSGKGIQLKYYLFTQSFN 180

Query: 181 VISRMVLGKKYLEQSKEYSIFSPDEFRKMLEELFFLHGALNIGDFIPWIDFLDLQGYVKR 240
           VISRMVLGKKYLEQSKEYSIFSPDEFRKMLEELFFLHGALNIGDFIPWIDFLDLQGYVKR
Sbjct: 181 VISRMVLGKKYLEQSKEYSIFSPDEFRKMLEELFFLHGALNIGDFIPWIDFLDLQGYVKR 240

Query: 241 MKALYKKFDRFLEHVLDEHNGRRMGVRDNYVAKDMVDVLLQLADDPNLEVKLERQGVKAI 300
           MKALYKKFDRFLEHVLDEHNGRRMGVRDNYVAKDMVDVLLQLADDPNLEVKLERQGVKAI
Sbjct: 241 MKALYKKFDRFLEHVLDEHNGRRMGVRDNYVAKDMVDVLLQLADDPNLEVKLERQGVKAI 300

Query: 301 TQDLIAAGTESSVVTIEWALSELLKRPDIFKKTTSELDKVVGRERWVEEKDIANLPYINA 360
           TQDLIAAGTESSVVTIEWALSELLKRPDIFKKTTSELDKVVGRERWVEEKDIANLPYINA
Sbjct: 301 TQDLIAAGTESSVVTIEWALSELLKRPDIFKKTTSELDKVVGRERWVEEKDIANLPYINA 360

Query: 361 IVKETMRLHPIAPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGRDPAIWENPNDFIPE 420
           IVKETMRLHPIAPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGRDPAIWENPNDFIPE
Sbjct: 361 IVKETMRLHPIAPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGRDPAIWENPNDFIPE 420

Query: 421 RFSGKSIDVKGQDFELLPLGSGRRMCPGYNLGLKVVQSSLANLLHGFVWKLEGDTKKEDL 480
           RFSGKSIDVKGQDFELLPLGSGRRMCPGYNLGLKVVQSSLANLLHGFVWKLEGDTKKEDL
Sbjct: 421 RFSGKSIDVKGQDFELLPLGSGRRMCPGYNLGLKVVQSSLANLLHGFVWKLEGDTKKEDL 480

Query: 481 NMDETFGLSTPKKFPLVVIAEPRLPPHLYSL 511
           NMDETFGLSTPKKFPLVVIAEPRLPPHLYSL
Sbjct: 481 NMDETFGLSTPKKFPLVVIAEPRLPPHLYSL 511

BLAST of MC08g2279 vs. ExPASy TrEMBL
Match: A0A6J1DF57 (cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111020252 PE=3 SV=1)

HSP 1 Score: 825 bits (2132), Expect = 8.84e-299
Identity = 398/511 (77.89%), Postives = 446/511 (87.28%), Query Frame = 0

Query: 1   MEAPCWLSHAALWVAIATLSLLLLSGRRRKLNLPPGPKPWPFIGNLNLIGPLPHHSIHQL 60
           ME P W+S+AA WVA   L LL    RRRKLNLPPGPKPWPFIGNLNLIG LPH SIHQL
Sbjct: 1   MELPSWVSYAAAWVATVALLLLSRRLRRRKLNLPPGPKPWPFIGNLNLIGSLPHQSIHQL 60

Query: 61  SKKHGPIMHLRFGSFPVVVASSIETAKLFLKTHDLNFISRPRLAAGKYIGYDYSDILWCP 120
           S+K+G IMHLRFGSFPVVV SS+E AK+FLKTHDL F+SRP+ AAGKY  Y+YSDI W  
Sbjct: 61  SRKYGSIMHLRFGSFPVVVGSSVEMAKIFLKTHDLTFVSRPKTAAGKYTTYNYSDITWSQ 120

Query: 121 YGPYWRQAQKICVTELFCAKRLDSLEYIRKEETNALLKEIYKFSGKGIQLKYYLFTQSFN 180
           YGPYWRQA+K+C+ ELF AKRLDS EYIR+EE +ALL++IY+  G+ IQ+K YL T S N
Sbjct: 121 YGPYWRQARKMCLMELFSAKRLDSYEYIRREEMSALLRDIYRSCGRAIQVKDYLSTVSLN 180

Query: 181 VISRMVLGKKYLEQSKEYSIFSPDEFRKMLEELFFLHGALNIGDFIPWIDFLDLQGYVKR 240
           VISRMVLGKKY ++S E +I SPDEF+KML+ELF L G LNIGD IPWIDFLDLQGY+KR
Sbjct: 181 VISRMVLGKKYTDES-ENAIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYIKR 240

Query: 241 MKALYKKFDRFLEHVLDEHNGRRMGVRDNYVAKDMVDVLLQLADDPNLEVKLERQGVKAI 300
           MKAL KKFDRFLEHVLDEHN RR G++D YVAKDMVDVLLQLADDP+LEVKLER GVKA 
Sbjct: 241 MKALSKKFDRFLEHVLDEHNERRKGIKD-YVAKDMVDVLLQLADDPDLEVKLERHGVKAF 300

Query: 301 TQDLIAAGTESSVVTIEWALSELLKRPDIFKKTTSELDKVVGRERWVEEKDIANLPYINA 360
           TQDLIA GTESS VT+EWA+SELLK+P+IF K T ELD+V+G+ERWVEEKDI NLPYI+A
Sbjct: 301 TQDLIAGGTESSAVTVEWAMSELLKKPEIFIKATEELDRVIGKERWVEEKDITNLPYIDA 360

Query: 361 IVKETMRLHPIAPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGRDPAIWENPNDFIPE 420
           I KETMRLHP+APMLVPR+SREDC+IAGYDIAK TR+LVNVWTIGRDP +WENPN+F PE
Sbjct: 361 IAKETMRLHPVAPMLVPRMSREDCKIAGYDIAKDTRILVNVWTIGRDPEVWENPNEFNPE 420

Query: 421 RFSGKSIDVKGQDFELLPLGSGRRMCPGYNLGLKVVQSSLANLLHGFVWKLEGDTKKEDL 480
           RF GKSIDVKGQDFELLP GSGRRMCPGY+LGLKV+QSSLANLLHGF WKL GD +KEDL
Sbjct: 421 RFIGKSIDVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFSWKLGGDMRKEDL 480

Query: 481 NMDETFGLSTPKKFPLVVIAEPRLPPHLYSL 511
           NM+E FGLSTPKKFPLV +AEPRLPPHLYS+
Sbjct: 481 NMEEIFGLSTPKKFPLVAVAEPRLPPHLYSM 509

BLAST of MC08g2279 vs. ExPASy TrEMBL
Match: A0A6J1GXG6 (cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111458046 PE=3 SV=1)

HSP 1 Score: 816 bits (2107), Expect = 6.56e-295
Identity = 394/508 (77.56%), Postives = 440/508 (86.61%), Query Frame = 0

Query: 4   PCWLSHAALWVAIATLSLLLLSGRRRKLNLPPGPKPWPFIGNLNLIGPLPHHSIHQLSKK 63
           P W+S+AA W+AI  L  L    RRR LNLPPGPKPWP IGNL+LIG LPH SIH LSKK
Sbjct: 8   PSWVSYAAAWLAILALFFLSRRLRRRNLNLPPGPKPWPLIGNLDLIGSLPHQSIHHLSKK 67

Query: 64  HGPIMHLRFGSFPVVVASSIETAKLFLKTHDLNFISRPRLAAGKYIGYDYSDILWCPYGP 123
           +GPIMHLRFGSFPVVV SS+E AK+FLKTHDLNF+SRP+ AAGKY  Y+YSDI W  YGP
Sbjct: 68  YGPIMHLRFGSFPVVVGSSVEMAKIFLKTHDLNFVSRPKTAAGKYTTYNYSDITWSQYGP 127

Query: 124 YWRQAQKICVTELFCAKRLDSLEYIRKEETNALLKEIYKFSGKGIQLKYYLFTQSFNVIS 183
           YWRQA+K+C+ ELF AKRLDS EYIR+EE NALL+++Y+  G  I++K YL T S NVIS
Sbjct: 128 YWRQARKMCLMELFSAKRLDSYEYIRREEMNALLRDVYRSCGDVIKVKDYLSTVSLNVIS 187

Query: 184 RMVLGKKYLEQSKEYSIFSPDEFRKMLEELFFLHGALNIGDFIPWIDFLDLQGYVKRMKA 243
           RMVLGKKY ++S E  I SPDEF+KML+ELF L G LNIGD IPWIDFLDLQGYVKRMKA
Sbjct: 188 RMVLGKKYTDES-ENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMKA 247

Query: 244 LYKKFDRFLEHVLDEHNGRRMGVRDNYVAKDMVDVLLQLADDPNLEVKLERQGVKAITQD 303
           L KKFDRFLEHVLDEHN RR GV+D YVA+DMVDVLLQLADDP+LEVKLER GVKA TQD
Sbjct: 248 LSKKFDRFLEHVLDEHNARREGVKD-YVAQDMVDVLLQLADDPDLEVKLERHGVKAFTQD 307

Query: 304 LIAAGTESSVVTIEWALSELLKRPDIFKKTTSELDKVVGRERWVEEKDIANLPYINAIVK 363
           LIA GTESS VT+EWA+SELL++P+I  K   ELD+V+G+ERWVEEKDIA+LPYI+AI K
Sbjct: 308 LIAGGTESSAVTVEWAMSELLRKPEILNKAREELDRVIGKERWVEEKDIASLPYIDAIAK 367

Query: 364 ETMRLHPIAPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGRDPAIWENPNDFIPERFS 423
           ETMRLHP+APMLVPR+ REDCQIAGYDIAKGTRVLVNVWTIGRDPA+WENPN+F PERF 
Sbjct: 368 ETMRLHPVAPMLVPRMCREDCQIAGYDIAKGTRVLVNVWTIGRDPAVWENPNEFSPERFM 427

Query: 424 GKSIDVKGQDFELLPLGSGRRMCPGYNLGLKVVQSSLANLLHGFVWKLEGDTKKEDLNMD 483
           GKSIDVKGQDFELLP GSGRRMCPGY+LGLKV+QSSLANLLHGF W L GD KKEDLNMD
Sbjct: 428 GKSIDVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFTWSLGGDMKKEDLNMD 487

Query: 484 ETFGLSTPKKFPLVVIAEPRLPPHLYSL 511
           E FGLSTPKKFPL V+A+PRLPPHLYS+
Sbjct: 488 EVFGLSTPKKFPLDVVAQPRLPPHLYSI 513

BLAST of MC08g2279 vs. ExPASy TrEMBL
Match: A0A6J1IRR8 (cytochrome P450 71A1-like OS=Cucurbita maxima OX=3661 GN=LOC111479921 PE=3 SV=1)

HSP 1 Score: 809 bits (2089), Expect = 3.61e-292
Identity = 390/508 (76.77%), Postives = 439/508 (86.42%), Query Frame = 0

Query: 4   PCWLSHAALWVAIATLSLLLLSGRRRKLNLPPGPKPWPFIGNLNLIGPLPHHSIHQLSKK 63
           P W+S+AA W+AI    LL    RRR LNLPPGPKPWP IGNL+LIG LPH SIH LSKK
Sbjct: 8   PSWVSYAAAWLAILAFLLLSRRLRRRNLNLPPGPKPWPLIGNLDLIGSLPHQSIHHLSKK 67

Query: 64  HGPIMHLRFGSFPVVVASSIETAKLFLKTHDLNFISRPRLAAGKYIGYDYSDILWCPYGP 123
           +GPIMHLRFGSFPVVV SS+E AK+FLKTHDLNF+SRP+ AAGKY  Y+YSDI W  YGP
Sbjct: 68  YGPIMHLRFGSFPVVVGSSVEMAKIFLKTHDLNFVSRPKTAAGKYTTYNYSDITWSQYGP 127

Query: 124 YWRQAQKICVTELFCAKRLDSLEYIRKEETNALLKEIYKFSGKGIQLKYYLFTQSFNVIS 183
           YWRQA+K+C+ ELF AKRLDS EYIR+EE NALL+++YK  G+ +++K YL T S NVIS
Sbjct: 128 YWRQARKMCLMELFSAKRLDSYEYIRREEMNALLRDVYKSCGEVMRVKDYLSTVSLNVIS 187

Query: 184 RMVLGKKYLEQSKEYSIFSPDEFRKMLEELFFLHGALNIGDFIPWIDFLDLQGYVKRMKA 243
           RMVLGKKY ++S E  I SPDEF+KML+ELF L G LNIGD IPWIDFLDLQGYVKRMKA
Sbjct: 188 RMVLGKKYTDES-ENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMKA 247

Query: 244 LYKKFDRFLEHVLDEHNGRRMGVRDNYVAKDMVDVLLQLADDPNLEVKLERQGVKAITQD 303
           L KKFDRFLEHVLDEHN RR GV+D YVAKDMVDVLLQLADDP+LEVKLER GVKA TQD
Sbjct: 248 LSKKFDRFLEHVLDEHNARREGVKD-YVAKDMVDVLLQLADDPHLEVKLERHGVKAFTQD 307

Query: 304 LIAAGTESSVVTIEWALSELLKRPDIFKKTTSELDKVVGRERWVEEKDIANLPYINAIVK 363
           LIA GTESS VT+EWA+SELL++P+I  K   ELD+V+G+ERWVEEKD+A+LPYI+AI K
Sbjct: 308 LIAGGTESSAVTVEWAMSELLRKPEILNKAREELDRVIGKERWVEEKDMASLPYIDAIAK 367

Query: 364 ETMRLHPIAPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGRDPAIWENPNDFIPERFS 423
           ETMRLHP+APMLVPR+ REDCQIAGYDI KGTRVLVNVWTIGRDPA+WENP++F PERF 
Sbjct: 368 ETMRLHPVAPMLVPRMCREDCQIAGYDIVKGTRVLVNVWTIGRDPAVWENPSEFSPERFM 427

Query: 424 GKSIDVKGQDFELLPLGSGRRMCPGYNLGLKVVQSSLANLLHGFVWKLEGDTKKEDLNMD 483
           GKSIDVKGQDFELLP GSGRRMCPGY+LGLKV+Q+SLANLLHGF W L  D KKEDLNMD
Sbjct: 428 GKSIDVKGQDFELLPFGSGRRMCPGYSLGLKVIQTSLANLLHGFTWSLGEDMKKEDLNMD 487

Query: 484 ETFGLSTPKKFPLVVIAEPRLPPHLYSL 511
           E FGLSTPKKFPL V+A+PRLPPHLYS+
Sbjct: 488 EIFGLSTPKKFPLDVVAQPRLPPHLYSI 513

BLAST of MC08g2279 vs. ExPASy TrEMBL
Match: A0A5D3C5I8 (Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold606G00890 PE=3 SV=1)

HSP 1 Score: 805 bits (2078), Expect = 1.77e-290
Identity = 391/508 (76.97%), Postives = 433/508 (85.24%), Query Frame = 0

Query: 4   PCWLSHAALWVAIATLSLLLLSGRRRKLNLPPGPKPWPFIGNLNLIGPLPHHSIHQLSKK 63
           P  LS+   W+A   L LL    RRRKLNLPPGPKPWP IGNL+LIG LPH SIHQLSKK
Sbjct: 7   PSCLSYITAWIAALALLLLSYRLRRRKLNLPPGPKPWPVIGNLHLIGSLPHQSIHQLSKK 66

Query: 64  HGPIMHLRFGSFPVVVASSIETAKLFLKTHDLNFISRPRLAAGKYIGYDYSDILWCPYGP 123
           +GPIMHLRFGSFPVVV SS+E AK+FLKT DLNF+SRP+ AAGKY  Y+YSDI W  YGP
Sbjct: 67  YGPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNYSDITWSQYGP 126

Query: 124 YWRQAQKICVTELFCAKRLDSLEYIRKEETNALLKEIYKFSGKGIQLKYYLFTQSFNVIS 183
           YWRQA+K+C+ ELF A+RLDS EYIRKEE N L++EIYK  G+ I++K YL T S NVIS
Sbjct: 127 YWRQARKMCLMELFSARRLDSYEYIRKEEMNGLVREIYKSCGEVIKVKDYLSTVSLNVIS 186

Query: 184 RMVLGKKYLEQSKEYSIFSPDEFRKMLEELFFLHGALNIGDFIPWIDFLDLQGYVKRMKA 243
           RMVLGKKY ++S E  I SPDEF+KML+ELF L G LNIGD IPWIDFLDLQGYVKRMKA
Sbjct: 187 RMVLGKKYTDESSENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMKA 246

Query: 244 LYKKFDRFLEHVLDEHNGRRMGVRDNYVAKDMVDVLLQLADDPNLEVKLERQGVKAITQD 303
           L KKFDRFLEHVLDEHN RR GV+ NYVAKDMVDVLLQLADDPNLEVKLER GVKA TQD
Sbjct: 247 LSKKFDRFLEHVLDEHNERRKGVQ-NYVAKDMVDVLLQLADDPNLEVKLERHGVKAFTQD 306

Query: 304 LIAAGTESSVVTIEWALSELLKRPDIFKKTTSELDKVVGRERWVEEKDIANLPYINAIVK 363
           LIA GTESS VT+EWA+SELLK P+IF K   EL++V+G+ERWVEEKDI NLPYINAI K
Sbjct: 307 LIAGGTESSAVTVEWAISELLKNPEIFNKAIEELNEVIGKERWVEEKDIVNLPYINAIAK 366

Query: 364 ETMRLHPIAPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGRDPAIWENPNDFIPERFS 423
           ETMRLHP+APMLVPR++REDCQIAGYDIAKGTRVLVNVWTIGRDP +W NP+ F P+RF 
Sbjct: 367 ETMRLHPVAPMLVPRMAREDCQIAGYDIAKGTRVLVNVWTIGRDPTVWRNPHAFDPDRFM 426

Query: 424 GKSIDVKGQDFELLPLGSGRRMCPGYNLGLKVVQSSLANLLHGFVWKLEGDTKKEDLNMD 483
             SIDVKGQDFELLP GSGRRMCPGY+LGLKV+QSSLANLLHGF WKL GD +KEDLNM+
Sbjct: 427 ESSIDVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFNWKLPGDMEKEDLNME 486

Query: 484 ETFGLSTPKKFPLVVIAEPRLPPHLYSL 511
           E FGLSTPKK+PL  +AEPRLPPHLYSL
Sbjct: 487 EIFGLSTPKKYPLDAVAEPRLPPHLYSL 513

BLAST of MC08g2279 vs. TAIR 10
Match: AT5G07990.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 419.1 bits (1076), Expect = 5.2e-117
Identity = 216/514 (42.02%), Postives = 310/514 (60.31%), Query Frame = 0

Query: 12  LWVAIATLSLLLL---SGRRRKLN---LPPGPKPWPFIGNLNLIGPLPHHSIHQLSKKHG 71
           L + +AT+  L+L   S RR + +   LPPGP PWP IGNL  +G  PH ++  +   +G
Sbjct: 6   LTILLATVLFLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKPHRTLSAMVTTYG 65

Query: 72  PIMHLRFGSFPVVVASSIETAKLFLKTHDLNFISRPRLAAGKYIGYDYSDILWCPYGPYW 131
           PI+HLR G   VVVA+S   A+ FLK HD NF SRP  +  K++ Y+Y D+++ PYG  W
Sbjct: 66  PILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPYGHRW 125

Query: 132 RQAQKICVTELFCAKRLDSLEYIRKEETNALLKEIYKFSGKGIQLKYYLFTQSFNVISRM 191
           R  +KI    LF AK L+  +++R+EE   L +E+ +   K + L   +     N + R 
Sbjct: 126 RLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVVNALGRE 185

Query: 192 VLGKKYLEQSKEYSIFSPDEFRKMLEELFFLHGALNIGDFIPWIDFLDLQGYVKRMKALY 251
           ++G++      ++     DEFR M+ E+  L G  NIGDF+P +D+LDLQG   +MK L+
Sbjct: 186 MIGRRLFGADADH---KADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKMKRLH 245

Query: 252 KKFDRFLEHVLDEH--NGRRMGVRDNYVAKDMVDVLLQL--ADDPNLEVKLERQGVKAIT 311
           K+FD FL  +L EH  NG+      +    DM+  L+ L   D       L    +KA+ 
Sbjct: 246 KRFDAFLSSILKEHEMNGQ------DQKHTDMLSTLISLKGTDLDGDGGSLTDTEIKALL 305

Query: 312 QDLIAAGTESSVVTIEWALSELLKRPDIFKKTTSELDKVVGRERWVEEKDIANLPYINAI 371
            ++  AGT++S  T++WA++EL++ PDI  K   ELD VVGR+R V E DIA LPY+ A+
Sbjct: 306 LNMFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQAV 365

Query: 372 VKETMRLHPIAPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGRDPAIWENPNDFIPER 431
           +KE  RLHP  P+ +P ++ E C+I GY I KG+ +L N+W I RDP  W +P  F PER
Sbjct: 366 IKENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPER 425

Query: 432 F----SGKSIDVKGQDFELLPLGSGRRMCPGYNLGLKVVQSSLANLLHGFVWKLEGDTKK 491
           F        +DVKG DFEL+P G+GRR+C G +LGL+ +Q   A L+ GF W+L G    
Sbjct: 426 FLPGGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTP 485

Query: 492 EDLNMDETFGLSTPKKFPLVVIAEPRLPPHLYSL 512
           E LNM+E++GL+  +  PLVV  +PRL P++Y L
Sbjct: 486 EKLNMEESYGLTLQRAVPLVVHPKPRLAPNVYGL 510

BLAST of MC08g2279 vs. TAIR 10
Match: AT3G26330.1 (cytochrome P450, family 71, subfamily B, polypeptide 37 )

HSP 1 Score: 360.1 bits (923), Expect = 2.8e-99
Identity = 197/498 (39.56%), Postives = 295/498 (59.24%), Query Frame = 0

Query: 12  LWVAIATLSLLLLSGRRRKLNLPPGPKPWPFIGNLNLIGPLPHHSIHQLSKKHGPIMHLR 71
           L+++   L+ L L  RR+    PP P  +P IGNL+ +G LPH S+  LSKK+GP+M L+
Sbjct: 10  LFLSCLLLAALRLKKRRQHQRKPPSPPGFPIIGNLHQLGELPHQSLWSLSKKYGPVMLLK 69

Query: 72  FGSFPVVVASSIETAKLFLKTHDLNFISRPRLAAGKYIGYDYSDILWCPYGPYWRQAQKI 131
           FGS P VV SS ETAK  LK HDLN  SRP LA  + + Y+Y DI++ P+  YW++ +++
Sbjct: 70  FGSIPTVVVSSSETAKQALKIHDLNCCSRPSLAGPRALSYNYLDIVFSPFNDYWKELRRM 129

Query: 132 CVTELFCAKRLDSLEYIRKEETNALLKEIYKFSGK--GIQLKYYLFTQSFNVISRMVLGK 191
           CV ELF  K++  ++ IR+EE   L+    + + +   + L   L + +  VI +   G 
Sbjct: 130 CVQELFSPKQVHLIQPIREEEVKKLMNSFSESAAQKTPVNLSEKLASLTVGVICKAAFGV 189

Query: 192 KYLEQSKEYSIFSPDEFRKMLEELFFLHGALNIGDFIP---W-IDFL-DLQGYVKRMKAL 251
            +     + ++ + D F K++ + F   G+ +  D+ P   W ID+L  LQG  +R    
Sbjct: 190 SF-----QGTVLNSDNFDKLIHDAFLFLGSFSASDYFPNVGWIIDWLTGLQGQRERS--- 249

Query: 252 YKKFDRFLEHVLDEH-NGRRMGVRDNYVAKDMVDVLLQLADDPNL--EVKLERQGVKAIT 311
            +  D F E + D H  G + GV      +D VD+LL+L  +  +    KL R  +KA+ 
Sbjct: 250 VRGLDAFYEQMFDLHKQGNKEGV------EDFVDLLLKLEKEETVLGYGKLTRNHIKAVL 309

Query: 312 QDLIAAGTESSVVTIEWALSELLKRPDIFKKTTSELDKVVGRERWVEEKDIANLPYINAI 371
            +++  G  +S +T+ WA++EL++ P + KK  SE+   +G +  +   DI  L Y+  +
Sbjct: 310 MNVLLGGIGTSAITMTWAMTELMRNPRVMKKVQSEIRNQIGGKSMICLDDIDQLHYLKMV 369

Query: 372 VKETMRLHPIAPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGRDPAIWENPNDFIPER 431
           + ET RLHP AP+LVPR    + +I GY I   TR+ VNVW IGRDP  W++P +F+PER
Sbjct: 370 INETWRLHPPAPLLVPREVMSEFEINGYTIPAKTRLYVNVWGIGRDPDTWKDPEEFLPER 429

Query: 432 FSGKSIDVKGQDFELLPLGSGRRMCPGYNLGLKVVQSSLANLLHGFVWKLEGDTKKEDLN 491
           F   +ID KGQ+FELLP GSGRRMCP   +G  +V+  LANLL+ F WKL      ED++
Sbjct: 430 FVNSNIDAKGQNFELLPFGSGRRMCPAMYMGTTMVEFGLANLLYHFDWKLPEGMVVEDID 489

Query: 492 MDETFGLSTPKKFPLVVI 500
           M+E+ GL+  KK  LV++
Sbjct: 490 MEESPGLNASKKNELVLV 493

BLAST of MC08g2279 vs. TAIR 10
Match: AT1G13080.1 (cytochrome P450, family 71, subfamily B, polypeptide 2 )

HSP 1 Score: 359.8 bits (922), Expect = 3.7e-99
Identity = 187/497 (37.63%), Postives = 292/497 (58.75%), Query Frame = 0

Query: 20  SLLLLSGRRRKLNLPPGPKPWPFIGNLNLIGPLPHHSIHQLSKKHGPIMHLRFGSFPVVV 79
           S+ L   +  K NLPP P   P IGNL+ +  LPH   H+LS K+GP++ LR GS PVVV
Sbjct: 18  SIFLKQNKTSKFNLPPSPSSLPIIGNLHHLAGLPHRCFHKLSIKYGPLVFLRLGSVPVVV 77

Query: 80  ASSIETAKLFLKTHDLNFISRPRLAAGKYIGYDYSDILWCPYGPYWRQAQKICVTELFCA 139
            SS E A+  LKT+DL   SRP+      + Y + DI + PYG YWR+ +K+ V ELF +
Sbjct: 78  ISSSEAAEAVLKTNDLECCSRPKTVGSGKLSYGFKDITFAPYGEYWREVRKLAVIELFSS 137

Query: 140 KRLDSLEYIRKEETNALLKEIYKFSGK--GIQLKYYLFTQSFNVISRMVLGKKYLEQSKE 199
           K++ S  YIR+EE + ++K++ + + K   + L    F+ + ++I R+ LG+ + E    
Sbjct: 138 KKVQSFRYIREEEVDFVVKKVSESALKQSPVDLSKTFFSLTASIICRVALGQNFNESG-- 197

Query: 200 YSIFSPDEFRKMLEELFFLHGALNIGDFIP-----WIDFLDLQGYVKRMKALYKKFDRFL 259
             +   D   +++ E     G     DF P     ++D+L  Q + K++  ++K+ D F 
Sbjct: 198 -FVIDQDRIEELVTESAEALGTFTFSDFFPGGLGRFVDWL-FQRH-KKINKVFKELDAFY 257

Query: 260 EHVLDEH---NGRRMGVRDNYVAKDMVDVLLQLAD--DPNLEVKLERQGVKAITQDLIAA 319
           +HV+D+H    GR+         +D+V ++L + D  + +   KL    +KAI  D+  A
Sbjct: 258 QHVIDDHLKPEGRK--------NQDIVTLILDMIDKQEDSDSFKLNMDNLKAIVMDVFLA 317

Query: 320 GTESSVVTIEWALSELLKRPDIFKKTTSELDKVVG-RERWVEEKDIANLPYINAIVKETM 379
           G ++S VT+ WA++EL++ P + KK    +   +G ++  + E+D+  + Y+N I+KET 
Sbjct: 318 GIDTSAVTMIWAMTELIRNPRVMKKAQESIRTTLGLKKERITEEDLGKVEYLNHILKETF 377

Query: 380 RLHPIAPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGRDPAIWENPNDFIPERFSGKS 439
           RLHP  P +VPR +    +I GYDI   T++ +NVWTIGRDP  W +P +F PERF+  S
Sbjct: 378 RLHPALPFVVPRETMSHIKIQGYDIPPKTQIQLNVWTIGRDPKRWNDPEEFNPERFANSS 437

Query: 440 IDVKGQDFELLPLGSGRRMCPGYNLGLKVVQSSLANLLHGFVWKLEGDTKKEDLNMDETF 499
           +D +GQ F+LLP GSGRR+CPG  + +  V+ +L NLL+ F W +   TK ED++M+E  
Sbjct: 438 VDFRGQHFDLLPFGSGRRICPGMPMAIASVELALMNLLYYFDWSMPDGTKGEDIDMEEAG 497

Query: 500 GLSTPKKFPLVVIAEPR 504
            +S  KK PL ++   R
Sbjct: 498 NISIVKKIPLQLVPVQR 501

BLAST of MC08g2279 vs. TAIR 10
Match: AT5G57260.1 (cytochrome P450, family 71, subfamily B, polypeptide 10 )

HSP 1 Score: 355.5 bits (911), Expect = 7.0e-98
Identity = 190/502 (37.85%), Postives = 299/502 (59.56%), Query Frame = 0

Query: 12  LWVA--IATLSLLLLS---GRRRKLNLPPGPKPWPFIGNLNLIGPLPHHSIHQLSKKHGP 71
           LW    I  +S+LL++    +RR +  PP P   P IGNL+ +G LPH S+ +LSKK+GP
Sbjct: 4   LWFVSLILLISILLVAVKHSKRRWVRQPPSPPGLPIIGNLHQLGELPHQSLCKLSKKYGP 63

Query: 72  IMHLRFGSFPVVVASSIETAKLFLKTHDLNFISRPRLAAGKYIGYDYSDILWCPYGPYWR 131
           +M L+ G  P V+ S+ ETAK  LK +DL+  SRP L   + + Y+Y DI +  +  YW+
Sbjct: 64  VMLLKLGRVPTVIVSTPETAKQVLKDYDLHCCSRPSLEGTRKLSYNYLDIAFSRFDDYWK 123

Query: 132 QAQKICVTELFCAKRLDSLEYIRKEETNALLKEIYKFSGKG--IQLKYYLFTQSFNVISR 191
           + +K+CV ELFC KR++S++ I++ E   L+  I + + +   + L     + + NVI +
Sbjct: 124 ELRKLCVEELFCNKRINSIQPIKEAEMEKLIDSIAESASQKTLVNLSDTFLSLNVNVICK 183

Query: 192 MVLGKKYLEQSKEYSIFSPDEFRKMLEELFFLHGALNIGDFIPWIDFLD--LQGYVKRMK 251
            V G  +     + ++ + D+F+ ++ E   + G+ +  DF P++ ++     G   R +
Sbjct: 184 AVFGVNF-----QGTVLNNDKFQDLVHEALEMLGSFSASDFFPYVGWIVDWFTGLHARRE 243

Query: 252 ALYKKFDRFLEHVLDEHNGRRMGVRDNYVAKDMVDVLLQLADDPNL--EVKLERQGVKAI 311
              +  D F E ++D H  +     ++    D VD+LL+L  +  +    KL R  +KAI
Sbjct: 244 RSVRDLDAFYEQMIDLHLQKNREESED----DFVDLLLRLEKEEAVLGYGKLTRNHIKAI 303

Query: 312 TQDLIAAGTESSVVTIEWALSELLKRPDIFKKTTSELDKVVGRE---RWVEEKDIANLPY 371
             +++  G  +S +T+ WA++EL++ P + KK  SE+   +G+    R +   +I +L Y
Sbjct: 304 LMNILLGGINTSAITMTWAMAELIRNPRVMKKVQSEIRAQIGKNNKTRIISLDEINHLSY 363

Query: 372 INAIVKETMRLHPIAPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGRDPAIWENPNDF 431
           +N ++KET RLHP+AP+LVPR    + +I GY I   TR+ VNVW IGRDP IW++P +F
Sbjct: 364 LNMVIKETCRLHPVAPLLVPREVISEFKINGYTIQPKTRLHVNVWAIGRDPEIWKDPEEF 423

Query: 432 IPERFSGKSIDVKGQDFELLPLGSGRRMCPGYNLGLKVVQSSLANLLHGFVWKLEGDTKK 491
           +PERF    IDVKGQD+ELLP GSGRR+CP   +G+  V+  LANLL+ F WKL      
Sbjct: 424 LPERFMDCDIDVKGQDYELLPFGSGRRICPAVYMGITTVEFGLANLLYHFDWKLPEGVAV 483

Query: 492 EDLNMDETFGLSTPKKFPLVVI 500
           ED+ MDE  GL++ KK  L+++
Sbjct: 484 EDIYMDEASGLTSHKKHDLLLV 496

BLAST of MC08g2279 vs. TAIR 10
Match: AT2G40890.1 (cytochrome P450, family 98, subfamily A, polypeptide 3 )

HSP 1 Score: 352.8 bits (904), Expect = 4.5e-97
Identity = 185/507 (36.49%), Postives = 292/507 (57.59%), Query Frame = 0

Query: 14  VAIATLSLLLLSG--RRRKLNLPPGPKPWPFIGNLNLIGPLPHHSIHQLSKKHGPIMHLR 73
           +A+AT++ ++     +R +   PPGP P P +GNL  I P+     ++ ++ +GPI+ + 
Sbjct: 6   IAVATIAAVVSYKLIQRLRYKFPPGPSPKPIVGNLYDIKPVRFRCYYEWAQSYGPIISVW 65

Query: 74  FGSFPVVVASSIETAKLFLKTHDLNFISRPRLAAGKYIGYDYSDILWCPYGPYWRQAQKI 133
            GS   VV SS E AK  LK HD     R R  + +    +  D++W  YGP++ + +K+
Sbjct: 66  IGSILNVVVSSAELAKEVLKEHDQKLADRHRNRSTEAFSRNGQDLIWADYGPHYVKVRKV 125

Query: 134 CVTELFCAKRLDSLEYIRKEETNALLKEIYK------FSGKGIQLKYYLFTQSFNVISRM 193
           C  ELF  KRL+SL  IR++E  A+++ +++         KG+QL+ YL   +FN I+R+
Sbjct: 126 CTLELFTPKRLESLRPIREDEVTAMVESVFRDCNLPENRAKGLQLRKYLGAVAFNNITRL 185

Query: 194 VLGKKYLEQS---KEYSIFSPDEFRKMLEELFFLHGALNIGDFIPWIDFLDLQGYVKRMK 253
             GK+++       E  +    EF+ ++     L  +L+I + IPW+ ++      K   
Sbjct: 186 AFGKRFMNAEGVVDEQGL----EFKAIVSNGLKLGASLSIAEHIPWLRWM-FPADEKAFA 245

Query: 254 ALYKKFDRFLEHVLDEHNGRRMGVRDNYVAKDMVDVLLQLADDPNLEVKLERQGVKAITQ 313
               + DR    +++EH   R   + +   +  VD LL L D    +  L    +  +  
Sbjct: 246 EHGARRDRLTRAIMEEHTLARQ--KSSGAKQHFVDALLTLKD----QYDLSEDTIIGLLW 305

Query: 314 DLIAAGTESSVVTIEWALSELLKRPDIFKKTTSELDKVVGRERWVEEKDIANLPYINAIV 373
           D+I AG +++ +T EWA++E++K P + +K   E D+VVG +R + E D + LPY+  +V
Sbjct: 306 DMITAGMDTTAITAEWAMAEMIKNPRVQQKVQEEFDRVVGLDRILTEADFSRLPYLQCVV 365

Query: 374 KETMRLHPIAPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGRDPAIWENPNDFIPERF 433
           KE+ RLHP  P+++P  S  D +I GYDI KG+ V VNVW + RDPA+W+NP +F PERF
Sbjct: 366 KESFRLHPPTPLMLPHRSNADVKIGGYDIPKGSNVHVNVWAVARDPAVWKNPFEFRPERF 425

Query: 434 SGKSIDVKGQDFELLPLGSGRRMCPGYNLGLKVVQSSLANLLHGFVWKLEGDTKKEDLNM 493
             + +D+KG DF LLP G+GRR+CPG  LG+ +V S +++LLH FVW     TK E+++M
Sbjct: 426 LEEDVDMKGHDFRLLPFGAGRRVCPGAQLGINLVTSMMSHLLHHFVWTPPQGTKPEEIDM 485

Query: 494 DETFGLSTPKKFPLVVIAEPRLPPHLY 510
            E  GL T  + P+  +A PRLP  LY
Sbjct: 486 SENPGLVTYMRTPVQAVATPRLPSDLY 501