MC08g2262 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g2262 vs. ExPASy Swiss-Prot
Match: Q40541 (Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097 GN=NPK1 PE=1 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 9.0e-205
Identity = 408/702 (58.12%), Postives = 498/702 (70.94%), Query Frame = 0

Query: 32  MQDIFASVRRSLVFRAPLDNEDSHTPPVGVGVGALVDKINSGIRKSR--VFSR-HSPSSA 91
           MQD   SVRRSLVF+   D  D+    VG G G  V+K+ S IRKS   +FS+ H P  A
Sbjct: 1   MQDFIGSVRRSLVFKQSGD-FDTGAAGVGSGFGGFVEKLGSSIRKSSIGIFSKAHVP--A 60

Query: 92  LPPIP---------KENEPPIRWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVLIAANG 151
           LP I          K++ PPIRWRKGE+IGCGAFGRVYMGMN+DSGELLA+K+V IA NG
Sbjct: 61  LPSISKAELPAKARKDDTPPIRWRKGEMIGCGAFGRVYMGMNVDSGELLAIKEVSIAMNG 120

Query: 152 ASKEKAQAHIKELEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKF 211
           AS+E+AQAH++ELEEEV LLK+LSHPNIVRYLGT RE  SLNILLEFVPGGSISSLLGKF
Sbjct: 121 ASRERAQAHVRELEEEVNLLKNLSHPNIVRYLGTAREAGSLNILLEFVPGGSISSLLGKF 180

Query: 212 EAFPEAVIRTYTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVAELA 271
            +FPE+VIR YTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASK+V ELA
Sbjct: 181 GSFPESVIRMYTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELA 240

Query: 272 TVSGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSDQYPEVAALFH 331
           T++GAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCT+IEMATGKPPWS QY EVAALFH
Sbjct: 241 TMTGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTIIEMATGKPPWSQQYQEVAALFH 300

Query: 332 IGTTKSHPPIPEQLSIEAQDFLLKCLQKEPNLRPVASELLKHPFVTGEEAKSQLASNNAC 391
           IGTTKSHPPIPE LS E++DFLLKCLQKEP+LR  AS LL+HPFVT E  +++    ++ 
Sbjct: 301 IGTTKSHPPIPEHLSAESKDFLLKCLQKEPHLRHSASNLLQHPFVTAEHQEARPFLRSSF 360

Query: 392 MICHHQEPLEIHSPLYTANLEMSKTSTHLGSSDICNLDSLSCSKVYTGNE-QENDIWGTN 451
           M   + E +         ++     ++  G  D+C + ++ CS VY  N   +  +W   
Sbjct: 361 M--GNPENMAAQRMDVRTSIIPDMRASCNGLKDVCGVSAVRCSTVYPENSLGKESLWKLG 420

Query: 452 NSDDEMCQIDDKDDFMLD-EVKLRSSI-IPENLKSYNPICEPSDDEDCKFDKGPVVDQGS 511
           NSDD+MCQ+D+ DDFM    VK  S +  P N KS+NP+CEP +D  CKFD+ P + +  
Sbjct: 421 NSDDDMCQMDN-DDFMFGASVKCSSDLHSPANYKSFNPMCEPDNDWPCKFDESPELTKSQ 480

Query: 512 ---NLDEKALAPGSCSGV-FDEDQNFSFPCGRSLSEDEDEVTESKIKAFLDEKAFELKKM 571
              + D+  + P +   + + ED  F+FP G+S +ED+DE+TESKI+AFLDEKA +LKK+
Sbjct: 481 ANLHYDQATIKPTNNPIMSYKEDLAFTFPSGQSAAEDDDELTESKIRAFLDEKAMDLKKL 540

Query: 572 QTPLYEEFYNSLNTSCSPVFIESKPDESTPKYLKLPPKSRSPSRSPGNQ-STVMDAFGTG 631
           QTPLYE FYNSLN S +P  + +   E+ P  + LPPKSRSP R    + ST ++     
Sbjct: 541 QTPLYEGFYNSLNVSSTPSPVGTGNKENVPSNINLPPKSRSPKRMLSRRLSTAIEGACAP 600

Query: 632 SPGSGSRSNGN--------DQRSQL---NDWKGLLADGQSEAGSPRKHYSEIQRKWKEEL 691
           SP + S+   N         Q +QL   N+WK LL    S+  +    +SE QR+WKEEL
Sbjct: 601 SPVTHSKRISNIGGLNGEAIQEAQLPRHNEWKDLLG---SQREAVNSSFSERQRRWKEEL 660

Query: 692 DQELERKREMMRQAGGGSKTSSPKDKAIGRQRERTRFASPLR 703
           D+EL+RKRE+MRQA      S PKD  + R R ++RFASP R
Sbjct: 661 DEELQRKREIMRQA---VNLSPPKDPILNRCRSKSRFASPGR 690

BLAST of MC08g2262 vs. ExPASy Swiss-Prot
Match: O22040 (Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3702 GN=ANP1 PE=1 SV=2)

HSP 1 Score: 686.4 bits (1770), Expect = 3.4e-196
Identity = 413/696 (59.34%), Postives = 493/696 (70.83%), Query Frame = 0

Query: 32  MQDIFASVRRSLVFRAPLDNEDSHTPPVGVGVGALVDKINSGIRKSRVFSRHSPSSALPP 91
           MQD F SVRRSLVFR   D+++    P   GV  L DKI S IRKS++F + S S   P 
Sbjct: 1   MQDFFGSVRRSLVFRPSSDDDNQENQPPFPGV--LADKITSCIRKSKIFIKPSFSPPPPA 60

Query: 92  IPKENEPPIRWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVLIAANGASKEKAQAHIKE 151
              +  PPI WRKG+LIG GAFG VYMGMNLDSGELLAVKQVLIAAN ASKEK QAHI+E
Sbjct: 61  NTVDMAPPISWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQE 120

Query: 152 LEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFEAFPEAVIRTYT 211
           LEEEVKLLK+LSHPNIVRYLGTVREDD+LNILLEFVPGGSISSLL KF  FPE+V+RTYT
Sbjct: 121 LEEEVKLLKNLSHPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYT 180

Query: 212 KQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVAELATVSGAKSMKGTP 271
           +QLLLGLEYLH + IMHRDIKGANILVDNKGCIKLADFGASKQVAELAT++GAKSMKGTP
Sbjct: 181 RQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTP 240

Query: 272 YWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSDQYPEVAALFHIGTTKSHPPIPE 331
           YWMAPEVILQTGHSFSADIWSVGCTVIEM TGK PWS QY EVAA+F IGTTKSHPPIP+
Sbjct: 241 YWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPD 300

Query: 332 QLSIEAQDFLLKCLQKEPNLRPVASELLKHPFVTGEEAKSQLASNNACMICHHQEPLEIH 391
            LS +A+DFLLKCLQ+ PNLRP ASELLKHPFV G+  +S  AS +   + ++   L   
Sbjct: 301 TLSSDAKDFLLKCLQEVPNLRPTASELLKHPFVMGKHKES--ASTDLGSVLNN---LSTP 360

Query: 392 SPLYTANLEMSKTSTHLGSSDICNLDSLSCSKV-YTGNEQENDIWGTNNS---DDEMCQI 451
            PL   N + +  ST     D+CN  SL+ S V    + Q  ++W  N++   +D+MC I
Sbjct: 361 LPLQINNTKSTPDSTCDDVGDMCNFGSLNYSLVDPVKSIQNKNLWQQNDNGGDEDDMCLI 420

Query: 452 DDKDDFMLDEVKLRSSIIPE--NLKSYNPICEPSDDEDCKFDKGPVVDQGSNLDEKALAP 511
           DD ++F+  + ++ S++  +    KS + I + S     KFD+ P               
Sbjct: 421 DD-ENFLTFDGEMSSTLEKDCHLKKSCDDISDMSIALKSKFDESP--------------- 480

Query: 512 GSCSGVFDEDQNFSFPCGR-SLSEDEDEVTESKIKAFLDEKAFELKKMQTPLYEEFYNSL 571
               G  +++   S  C + S SED+DE+TESKIKAFLDEKA +LKK+QTPLYEEFYNSL
Sbjct: 481 ----GNGEKESTMSMECDQPSYSEDDDELTESKIKAFLDEKAADLKKLQTPLYEEFYNSL 540

Query: 572 NTSCSPVFIE-----SKPDESTPKYLKLPPKSRSPSRSP--GNQSTVMDAFG-TGSPGSG 631
            T  SP  +E     SK +++   +LKLPPKSRSPSR P  G+ S   DA   + SPGSG
Sbjct: 541 IT-FSPSCMESNLSNSKREDTARGFLKLPPKSRSPSRGPLGGSPSRATDATSCSKSPGSG 600

Query: 632 SR-----SNGNDQRSQ------LNDWKGLLADGQSEAGSPRKHYSEIQRKWKEELDQELE 691
                  +NG D+ SQ      + DW+GL+ D + E  S     SEI++KWKEELDQELE
Sbjct: 601 GSRELNINNGGDEASQDGVSARVTDWRGLVVDTKQEL-SQCVALSEIEKKWKEELDQELE 660

Query: 692 RKR-EMMRQAGGGSKTSSPKDKAIGRQRERTRFASP 701
           RKR E+MRQAG G   SSP+D+ + RQRE++RFASP
Sbjct: 661 RKRQEIMRQAGLG---SSPRDRGMSRQREKSRFASP 664

BLAST of MC08g2262 vs. ExPASy Swiss-Prot
Match: Q9FZ36 (Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=ANP2 PE=2 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 3.0e-192
Identity = 411/699 (58.80%), Postives = 487/699 (69.67%), Query Frame = 0

Query: 32  MQDIFASVRRSLVFRAPLD--NEDSHTPPVGVGVGALVDKINSGIRKSRVF--SRHSPSS 91
           MQD+F SVRRSLVFR+  D  N+++H PP       L DKI S IRKS VF  S+  P++
Sbjct: 1   MQDLFGSVRRSLVFRSTTDDENQENHPPPF---PSLLADKITSCIRKSMVFAKSQSPPNN 60

Query: 92  ALPPIPKENEPPIRWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVLIAANGASKEKAQA 151
           +   I    +PPIRWRKG+LIG GAFG VYMGMNLDSGELLAVKQVLI +N ASKEK QA
Sbjct: 61  STVQI----KPPIRWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCASKEKTQA 120

Query: 152 HIKELEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFEAFPEAVI 211
           HI+ELEEEVKLLK+LSHPNIVRYLGTVRED++LNILLEFVPGGSISSLL KF AFPE+V+
Sbjct: 121 HIQELEEEVKLLKNLSHPNIVRYLGTVREDETLNILLEFVPGGSISSLLEKFGAFPESVV 180

Query: 212 RTYTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVAELATVSGAKSM 271
           RTYT QLLLGLEYLH + IMHRDIKGANILVDN+GCIKLADFGASKQVAELAT+SGAKSM
Sbjct: 181 RTYTNQLLLGLEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATISGAKSM 240

Query: 272 KGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSDQYPEVAALFHIGTTKSHP 331
           KGTPYWMAPEVILQTGHSFSADIWSVGCTVIEM TGK PWS QY E+AA+FHIGTTKSHP
Sbjct: 241 KGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKSHP 300

Query: 332 PIPEQLSIEAQDFLLKCLQKEPNLRPVASELLKHPFVTG---EEAKSQLAS--NNACMIC 391
           PIP+ +S +A DFLLKCLQ+EPNLRP ASELLKHPFVTG   E A   L S  +N+C   
Sbjct: 301 PIPDNISSDANDFLLKCLQQEPNLRPTASELLKHPFVTGKQKESASKDLTSFMDNSC--- 360

Query: 392 HHQEPLEIHSPLYTANLEMSKTSTHLGSSDICNLDSLSCSKVYTGNEQEND--IWGTNN- 451
               PL    P    N+   +TST     DICNL SL+C+  +     +N+     +NN 
Sbjct: 361 ---SPL----PSELTNITSYQTSTSDDVGDICNLGSLTCTLAFPEKSIQNNSLCLKSNNG 420

Query: 452 ----SDDEMCQIDDKDDFMLDEVKLRSSI--IPENLKSYNPICEPSDDEDCKFDKGPVVD 511
                D++MC IDD ++F+    +   S+    +  KS + + E SD   CKFD+     
Sbjct: 421 YDDDDDNDMCLIDD-ENFLTYNGETGPSLDNNTDAKKSCDTMSEISDILKCKFDEN---- 480

Query: 512 QGSNLDEKALAPGSCSGVFDEDQNFSFPCGR-SLSEDEDEVTESKIKAFLDEKAFELKKM 571
                          SG  + +   S      S SEDE+E+TESKIKAFLD+KA ELKK+
Sbjct: 481 ---------------SGNGETETKVSMEVDHPSYSEDENELTESKIKAFLDDKAAELKKL 540

Query: 572 QTPLYEEFYNSLNTSCSPVFIES-----KPDESTPKYLKLPPKSRSPS-----RSPGNQS 631
           QTPLYEEFYN + T CSP+ +ES     K +E+   +LKLPPKSRSPS     RSP   +
Sbjct: 541 QTPLYEEFYNGMIT-CSPICMESNINNNKREEAPRGFLKLPPKSRSPSQGHIGRSPSRAT 600

Query: 632 TVMDAFGTGSPGSGSRSNGNDQRSQLNDWKGLLADGQSEAGSPRKHYSEIQRKWKEELDQ 691
               A  + SP SG+ S+G  + S  +      A  + E+ S     SEI+RKWKEELDQ
Sbjct: 601 DA--ACCSKSPESGN-SSGAPKNSNAS------AGAEQESNSQSVALSEIERKWKEELDQ 649

Query: 692 ELERK-REMMRQAGGGSKTSSPKDKAIGRQRERTRFASP 701
           ELERK RE+ RQAG G   SSP+D+++ R RE++RFASP
Sbjct: 661 ELERKRREITRQAGMG---SSPRDRSLSRHREKSRFASP 649

BLAST of MC08g2262 vs. ExPASy Swiss-Prot
Match: O22042 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=ANP3 PE=1 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 1.2e-180
Identity = 361/655 (55.11%), Postives = 449/655 (68.55%), Query Frame = 0

Query: 32  MQDIFASVRRSLVFRAPLDNEDSHTPPVGVGVGALVDKINSGIRKSRVFSRHSPSSALPP 91
           MQDI  SVRRSLVFR+ L  +D  +   G G+   V KINS IR SR+     P   LP 
Sbjct: 1   MQDILGSVRRSLVFRSSLAGDDGTS---GGGLSGFVGKINSSIRSSRIGLFSKPPPGLPA 60

Query: 92  IPKENEPPIRWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVLIAANGASKEKAQAHIKE 151
             KE  P IRWRKGELIGCGAFGRVYMGMNLDSGELLA+KQVLIA + ASKEK Q HI+E
Sbjct: 61  PRKEEAPSIRWRKGELIGCGAFGRVYMGMNLDSGELLAIKQVLIAPSSASKEKTQGHIRE 120

Query: 152 LEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFEAFPEAVIRTYT 211
           LEEEV+LLK+LSHPNIVRYLGTVRE DSLNIL+EFVPGGSISSLL KF +FPE VI  YT
Sbjct: 121 LEEEVQLLKNLSHPNIVRYLGTVRESDSLNILMEFVPGGSISSLLEKFGSFPEPVIIMYT 180

Query: 212 KQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVAELATVSGAKSMKGTP 271
           KQLLLGLEYLH NGIMHRDIKGANILVDNKGCI+LADFGASK+V ELATV+GAKSMKGTP
Sbjct: 181 KQLLLGLEYLHNNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATVNGAKSMKGTP 240

Query: 272 YWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSDQYPEVAALFHIGTTKSHPPIPE 331
           YWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWS+QY + AA+ HIG TK+HPPIPE
Sbjct: 241 YWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTKAHPPIPE 300

Query: 332 QLSIEAQDFLLKCLQKEPNLRPVASELLKHPFVTGEEAKSQLASNNACMICHHQEPLEIH 391
            LS EA+DFL+KCL KEP+LR  A+ELL+HPFVTG+  +   A  N+   C +    +  
Sbjct: 301 DLSPEAKDFLMKCLHKEPSLRLSATELLQHPFVTGKRQEPYPAYRNSLTECGNPITTQGM 360

Query: 392 SPLYTANLEMSKTSTHLGSSDICNLDSLSCSKVYTGNEQENDIWGTNNSDDEMCQIDDKD 451
           +   + N  + + ST  G  D+C L SL  S +Y      +     +   D++CQ D  D
Sbjct: 361 NVRSSIN-SLIRRSTCSGLKDVCELGSLRSSIIYPQKSNNSGFGWRDGDSDDLCQTDMDD 420

Query: 452 DFMLDEVKLRSSIIPENL---KSYNPICEPSDDEDCKFDKGP-VVDQGSNLDEKALAPGS 511
              ++ V  R++++ ++    KS+NP+C+ +D+  CKFD+ P V+   SNL     +   
Sbjct: 421 LCNIESV--RNNVLSQSTDLNKSFNPMCDSTDNWSCKFDESPKVMKSKSNLLSYQASQLQ 480

Query: 512 CSGVFDEDQNFSFPCGRSLSEDEDEVTESKIKAFLDEKAFELKKMQTPLYEEFYNSLNTS 571
                DE+ + +F  G S++ED+ + TE KIK+FLDEKA +LK++QTPL EEF+N++N  
Sbjct: 481 TGVPCDEETSLTFAGGSSVAEDDYKGTELKIKSFLDEKAQDLKRLQTPLLEEFHNAMNPG 540

Query: 572 CSPVFIESKPDESTPKYLKLPPKSRSPSRSPGNQ-STVMDAFGTGSPGSGSRSNGNDQRS 631
                +      + P    LP  S++P R P  + S + DA    SP   S+   N  R 
Sbjct: 541 IPQGALGDTNIYNLP---NLPSISKTPKRLPSRRLSAISDAM--PSPLKSSKRTLNTSRV 600

Query: 632 QLNDWKGLLADGQSEAG-SPRKHYSEIQRKWKEELDQELERKREMMRQAGGGSKT 681
             +  +    +  ++ G +  + +SEI+RKW+EEL +ELER RE +R AG G KT
Sbjct: 601 MQSGTEPTQVNESTKKGVNNSRCFSEIRRKWEEELYEELERHRENLRHAGAGGKT 644

BLAST of MC08g2262 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 301.6 bits (771), Expect = 2.4e-80
Identity = 146/274 (53.28%), Postives = 197/274 (71.90%), Query Frame = 0

Query: 100 IRWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVLIAANGASKEKAQAHIKELEEEVKLL 159
           I+W+KG+++G G +G VY+G+N D+GEL AVKQ+ I  +  S  K +  I    +E++++
Sbjct: 168 IKWQKGQILGRGGYGSVYLGLNKDTGELFAVKQLEI-VDINSDPKLKNMILSFSKEIEVM 227

Query: 160 KDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFEAFPEAVIRTYTKQLLLGLE 219
           + L H NIVRYLGT  +   L++ LE++PGGSISSLLGKF AF E VI+ YTKQ+L GL 
Sbjct: 228 RSLRHDNIVRYLGTSLDQSFLSVFLEYIPGGSISSLLGKFGAFSENVIKVYTKQILQGLS 287

Query: 220 YLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVAELATVSGAKSMKGTPYWMAPEVI 279
           +LH N I+HRDIKGANIL+D KG +KL+DFG SK  + +  VS  KSM+GTPYWMAPEVI
Sbjct: 288 FLHANSIIHRDIKGANILIDTKGIVKLSDFGCSKSFSGI--VSQFKSMQGTPYWMAPEVI 347

Query: 280 LQTGHSFSADIWSVGCTVIEMATGKPPWSDQYPEVAALFHIGTTKSHPPIPEQLSIEAQD 339
            QTGH  S+DIWS+GC ++EMAT +PPWS+     A ++HI ++ S P IP  +S EA D
Sbjct: 348 KQTGHGRSSDIWSLGCVIVEMATAQPPWSNITELAAVMYHIASSNSIPNIPSHMSQEAFD 407

Query: 340 FLLKCLQKEPNLRPVASELLKHPFVTGEEAKSQL 374
           FL  C +++P  RP A++LLKHPF+   +   QL
Sbjct: 408 FLNLCFKRDPKERPDANQLLKHPFIMNLDDNIQL 438

BLAST of MC08g2262 vs. NCBI nr
Match: XP_022150775.1 (mitogen-activated protein kinase kinase kinase NPK1 [Momordica charantia])

HSP 1 Score: 1346 bits (3483), Expect = 0.0
Identity = 682/687 (99.27%), Postives = 682/687 (99.27%), Query Frame = 0

Query: 32  MQDIFASVRRSLVFRAPLDNEDSHTPPVGVGVGALVDKINSGIRKSRVFSRHSPSSALPP 91
           MQDIFASVRRSLVFRAPLDNEDSHTPPVGVGVGALVDKINSGIRKSRVFSRHSPSSALPP
Sbjct: 1   MQDIFASVRRSLVFRAPLDNEDSHTPPVGVGVGALVDKINSGIRKSRVFSRHSPSSALPP 60

Query: 92  IPKENEPPIRWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVLIAANGASKEKAQAHIKE 151
           IPKENEPPIRWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVLIAANGASKEKAQAHIKE
Sbjct: 61  IPKENEPPIRWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVLIAANGASKEKAQAHIKE 120

Query: 152 LEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFEAFPEAVIRTYT 211
           LEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFEAFPEAVIRTYT
Sbjct: 121 LEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFEAFPEAVIRTYT 180

Query: 212 KQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVAELATVSGAKSMKGTP 271
           KQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVAELATVSGAKSMKGTP
Sbjct: 181 KQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVAELATVSGAKSMKGTP 240

Query: 272 YWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSDQYPEVAALFHIGTTKSHPPIPE 331
           YWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSDQYPEVAALFHIGTTKSHPPIPE
Sbjct: 241 YWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSDQYPEVAALFHIGTTKSHPPIPE 300

Query: 332 QLSIEAQDFLLKCLQKEPNLRPVASELLKHPFVTGEEAKSQLASNNACMICHHQEPLEIH 391
           QLSIEAQDFLLKCLQKEPNLRPVASELLKHPFVTGEEAKSQLASNNACM     EPLEIH
Sbjct: 301 QLSIEAQDFLLKCLQKEPNLRPVASELLKHPFVTGEEAKSQLASNNACM-----EPLEIH 360

Query: 392 SPLYTANLEMSKTSTHLGSSDICNLDSLSCSKVYTGNEQENDIWGTNNSDDEMCQIDDKD 451
           SPLYTANLEMSKTSTHLGSSDICNLDSLSCSKVYTGNEQENDIWGTNNSDDEMCQIDDKD
Sbjct: 361 SPLYTANLEMSKTSTHLGSSDICNLDSLSCSKVYTGNEQENDIWGTNNSDDEMCQIDDKD 420

Query: 452 DFMLDEVKLRSSIIPENLKSYNPICEPSDDEDCKFDKGPVVDQGSNLDEKALAPGSCSGV 511
           DFMLDEVKLRSSIIPENLKSYNPICEPSDDEDCKFDKGPVVDQGSNLDEKALAPGSCSGV
Sbjct: 421 DFMLDEVKLRSSIIPENLKSYNPICEPSDDEDCKFDKGPVVDQGSNLDEKALAPGSCSGV 480

Query: 512 FDEDQNFSFPCGRSLSEDEDEVTESKIKAFLDEKAFELKKMQTPLYEEFYNSLNTSCSPV 571
           FDEDQNFSFPCGRSLSEDEDEVTESKIKAFLDEKAFELKKMQTPLYEEFYNSLNTSCSPV
Sbjct: 481 FDEDQNFSFPCGRSLSEDEDEVTESKIKAFLDEKAFELKKMQTPLYEEFYNSLNTSCSPV 540

Query: 572 FIESKPDESTPKYLKLPPKSRSPSRSPGNQSTVMDAFGTGSPGSGSRSNGNDQRSQLNDW 631
           FIESKPDESTPKYLKLPPKSRSPSRSPGNQSTVMDAFGTGSPGSGSRSNGNDQRSQLNDW
Sbjct: 541 FIESKPDESTPKYLKLPPKSRSPSRSPGNQSTVMDAFGTGSPGSGSRSNGNDQRSQLNDW 600

Query: 632 KGLLADGQSEAGSPRKHYSEIQRKWKEELDQELERKREMMRQAGGGSKTSSPKDKAIGRQ 691
           KGLLADGQSEAGSPRKHYSEIQRKWKEELDQELERKREMMRQAGGGSKTSSPKDKAIGRQ
Sbjct: 601 KGLLADGQSEAGSPRKHYSEIQRKWKEELDQELERKREMMRQAGGGSKTSSPKDKAIGRQ 660

Query: 692 RERTRFASPLRDDASGTGKENRENIIS 718
           RERTRFASPLRDDASGTGKENRENIIS
Sbjct: 661 RERTRFASPLRDDASGTGKENRENIIS 682

BLAST of MC08g2262 vs. NCBI nr
Match: XP_022946570.1 (mitogen-activated protein kinase kinase kinase NPK1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1184 bits (3062), Expect = 0.0
Identity = 605/688 (87.94%), Postives = 633/688 (92.01%), Query Frame = 0

Query: 32  MQDIFASVRRSLVFRAPLDNEDSHTPPVGVGVGALVDKINSGIRKSRVFSRHSPSS-ALP 91
           MQDIFASVRRSLVFRAPL+NED H+P   +GVGALVDKINS IRKSRVFSRHSPSS ALP
Sbjct: 1   MQDIFASVRRSLVFRAPLENEDPHSP--AIGVGALVDKINSSIRKSRVFSRHSPSSSALP 60

Query: 92  PIPKENEPPIRWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVLIAANGASKEKAQAHIK 151
           PIPKE +PPIRWRKGELIGCGAFGRVYMGMNL SGELLAVKQVLIAANGASKEKAQ HIK
Sbjct: 61  PIPKETDPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQTHIK 120

Query: 152 ELEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFEAFPEAVIRTY 211
           ELEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKF AFPEAV+RTY
Sbjct: 121 ELEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFGAFPEAVLRTY 180

Query: 212 TKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVAELATVSGAKSMKGT 271
           TKQLLLGLEYLHKNGIMHRDIKGANILVDN GCIKLADFGASKQV ELAT+SGAKSMKGT
Sbjct: 181 TKQLLLGLEYLHKNGIMHRDIKGANILVDNNGCIKLADFGASKQVVELATISGAKSMKGT 240

Query: 272 PYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSDQYPEVAALFHIGTTKSHPPIP 331
           PYWMAPEVILQTGH+FSADIWSVGCTVIEMATGKPPWS QY EVAALFHIGTTKSHPPIP
Sbjct: 241 PYWMAPEVILQTGHNFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIP 300

Query: 332 EQLSIEAQDFLLKCLQKEPNLRPVASELLKHPFVTGEEAKSQLASNNACMICHHQEPLEI 391
           EQLS+EA+DFLLKCLQKEPNLRP ASELLKHPFVTGEEA+SQL S +AC      EPLEI
Sbjct: 301 EQLSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEEAESQLVSRDACT-----EPLEI 360

Query: 392 HSPLYTANLEMSKTSTHLGSSDICNLDSLSCSKVYTGNEQENDIWGTNNSDDEMCQIDDK 451
           HSPLYT+  EMSKT TH GSSDICNLDSLSCSKVYT N+ E+DIW T NSDDEMCQIDDK
Sbjct: 361 HSPLYTSKFEMSKTPTHPGSSDICNLDSLSCSKVYTANKLESDIW-TANSDDEMCQIDDK 420

Query: 452 DDFMLDEVKLRSSIIPENLKSYNPICEPSDDEDCKFDKGPVVDQGSNLDEKALAPGSCSG 511
           DD MLDEVK+ SSI+ ENLKSYNPICEPSDDEDCKFD+ P VDQGSNL+E+ALA  SCSG
Sbjct: 421 DDLMLDEVKIGSSIVHENLKSYNPICEPSDDEDCKFDRSPAVDQGSNLEEEALAARSCSG 480

Query: 512 VFDEDQNFSFPCGRSLSEDEDEVTESKIKAFLDEKAFELKKMQTPLYEEFYNSLNTSCSP 571
           VFDEDQNFSFP GRSLSEDEDE+TESKIKAFL EKA ELKK+Q+PLYEEFYNSLN SCSP
Sbjct: 481 VFDEDQNFSFPRGRSLSEDEDELTESKIKAFLYEKALELKKLQSPLYEEFYNSLNASCSP 540

Query: 572 VFIESKPDESTPKYLKLPPKSRSPSRSPGNQSTVMDAFGTGSPGSGSRSNGNDQRSQLND 631
           VFIESK DESTP YLKLPPKSRSPSRSPGN S  +DAFGTGSP SGSR N NDQRSQL+D
Sbjct: 541 VFIESKRDESTPTYLKLPPKSRSPSRSPGNPSPAIDAFGTGSPASGSRGNANDQRSQLSD 600

Query: 632 WKGLLADGQSEAGSPRKHYSEIQRKWKEELDQELERKREMMRQAGGGSKTSSPKDKAIGR 691
           WKGLL DGQSEAGSP KHYS+IQRKWKEELDQELE+KREMMRQAGGG KTSSPKDKA+GR
Sbjct: 601 WKGLLVDGQSEAGSPSKHYSDIQRKWKEELDQELEKKREMMRQAGGGGKTSSPKDKAMGR 660

Query: 692 QRERTRFASPLRDDASGTGKENRENIIS 718
            RERTRFASPLRDDASG G+ENRENIIS
Sbjct: 661 HRERTRFASPLRDDASGAGRENRENIIS 680

BLAST of MC08g2262 vs. NCBI nr
Match: KAG6599246.1 (Mitogen-activated protein kinase kinase kinase NPK1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1181 bits (3055), Expect = 0.0
Identity = 603/688 (87.65%), Postives = 633/688 (92.01%), Query Frame = 0

Query: 32  MQDIFASVRRSLVFRAPLDNEDSHTPPVGVGVGALVDKINSGIRKSRVFSRHSPSS-ALP 91
           MQDIFASVRRSLVFRAPL+NED H+P   +GVGALVDKINS IRKSRVFSRHSPSS ALP
Sbjct: 1   MQDIFASVRRSLVFRAPLENEDPHSP--AIGVGALVDKINSSIRKSRVFSRHSPSSSALP 60

Query: 92  PIPKENEPPIRWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVLIAANGASKEKAQAHIK 151
           PIPKE +PPIRWRKGELIGCGAFGRVYMGMNL SGELLAVKQVLIAANGASKEKAQ HIK
Sbjct: 61  PIPKETDPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQTHIK 120

Query: 152 ELEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFEAFPEAVIRTY 211
           ELEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKF AFPEAV+RTY
Sbjct: 121 ELEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFGAFPEAVLRTY 180

Query: 212 TKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVAELATVSGAKSMKGT 271
           TKQLLLGLEYLHKNGIMHRDIKGANILVDN GCIKLADFGASKQV ELAT+SGAKSMKGT
Sbjct: 181 TKQLLLGLEYLHKNGIMHRDIKGANILVDNNGCIKLADFGASKQVVELATISGAKSMKGT 240

Query: 272 PYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSDQYPEVAALFHIGTTKSHPPIP 331
           PYWMAPEVILQTGH+FSADIWSVGCTVIEMATGKPPWS QY EVAALFHIGTTKSHPPIP
Sbjct: 241 PYWMAPEVILQTGHNFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIP 300

Query: 332 EQLSIEAQDFLLKCLQKEPNLRPVASELLKHPFVTGEEAKSQLASNNACMICHHQEPLEI 391
           EQLS+EA+DFLLKCLQKEPNLRP ASELLKHPFVTGEEA+SQL S +AC      EPLEI
Sbjct: 301 EQLSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEEAESQLVSRDACT-----EPLEI 360

Query: 392 HSPLYTANLEMSKTSTHLGSSDICNLDSLSCSKVYTGNEQENDIWGTNNSDDEMCQIDDK 451
           HSPLYT+  EMSKT TH GSSDICNLDSLSCSK+YT N+ E+DIW T NSDDEMCQIDDK
Sbjct: 361 HSPLYTSKFEMSKTPTHPGSSDICNLDSLSCSKLYTANKLESDIW-TANSDDEMCQIDDK 420

Query: 452 DDFMLDEVKLRSSIIPENLKSYNPICEPSDDEDCKFDKGPVVDQGSNLDEKALAPGSCSG 511
           DD MLDEVK+ SSI+ ENLKSYNPICEPSDDEDCKFD+ P VD+GSNL+E+ALA  SCSG
Sbjct: 421 DDLMLDEVKIGSSIVHENLKSYNPICEPSDDEDCKFDRSPAVDRGSNLEEEALAARSCSG 480

Query: 512 VFDEDQNFSFPCGRSLSEDEDEVTESKIKAFLDEKAFELKKMQTPLYEEFYNSLNTSCSP 571
           VFDEDQNFSFP GRSLSEDEDE+TESKIKAFL EKA ELKK+Q+PLYEEFYNSLN SCSP
Sbjct: 481 VFDEDQNFSFPRGRSLSEDEDELTESKIKAFLYEKALELKKLQSPLYEEFYNSLNASCSP 540

Query: 572 VFIESKPDESTPKYLKLPPKSRSPSRSPGNQSTVMDAFGTGSPGSGSRSNGNDQRSQLND 631
           VFIESK DESTP YLKLPPKSRSPSRSPGN S  +DAFGTGSP SGSR N NDQRSQL+D
Sbjct: 541 VFIESKRDESTPTYLKLPPKSRSPSRSPGNPSPAIDAFGTGSPASGSRGNANDQRSQLSD 600

Query: 632 WKGLLADGQSEAGSPRKHYSEIQRKWKEELDQELERKREMMRQAGGGSKTSSPKDKAIGR 691
           WKGLL DGQSEAGSP KHYS+IQRKWKEELDQELE+KREMMRQAGGG KTSSPKDKA+GR
Sbjct: 601 WKGLLVDGQSEAGSPSKHYSDIQRKWKEELDQELEKKREMMRQAGGGGKTSSPKDKAMGR 660

Query: 692 QRERTRFASPLRDDASGTGKENRENIIS 718
            RERTRFASPLRDDASG G+ENRENIIS
Sbjct: 661 HRERTRFASPLRDDASGAGRENRENIIS 680

BLAST of MC08g2262 vs. NCBI nr
Match: XP_022946569.1 (mitogen-activated protein kinase kinase kinase NPK1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1179 bits (3050), Expect = 0.0
Identity = 605/689 (87.81%), Postives = 633/689 (91.87%), Query Frame = 0

Query: 32  MQDIFASVRRSLVFRAPLDNEDSHTPPVGVGVGALVDKINSGIRKSRVFSRHSPSS-ALP 91
           MQDIFASVRRSLVFRAPL+NED H+P   +GVGALVDKINS IRKSRVFSRHSPSS ALP
Sbjct: 1   MQDIFASVRRSLVFRAPLENEDPHSP--AIGVGALVDKINSSIRKSRVFSRHSPSSSALP 60

Query: 92  PIPKENEPPIRWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVLIAANGASKEKAQAHIK 151
           PIPKE +PPIRWRKGELIGCGAFGRVYMGMNL SGELLAVKQVLIAANGASKEKAQ HIK
Sbjct: 61  PIPKETDPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQTHIK 120

Query: 152 ELEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFEAFPEAVIRTY 211
           ELEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKF AFPEAV+RTY
Sbjct: 121 ELEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFGAFPEAVLRTY 180

Query: 212 TKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVAELATVSGAKSMKGT 271
           TKQLLLGLEYLHKNGIMHRDIKGANILVDN GCIKLADFGASKQV ELAT+SGAKSMKGT
Sbjct: 181 TKQLLLGLEYLHKNGIMHRDIKGANILVDNNGCIKLADFGASKQVVELATISGAKSMKGT 240

Query: 272 PYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSDQYPEVAALFHIGTTKSHPPIP 331
           PYWMAPEVILQTGH+FSADIWSVGCTVIEMATGKPPWS QY EVAALFHIGTTKSHPPIP
Sbjct: 241 PYWMAPEVILQTGHNFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIP 300

Query: 332 EQLSIEAQDFLLKCLQKEPNLRPVASELLKHPFVTGEEAKSQLASNNACMICHHQEPLEI 391
           EQLS+EA+DFLLKCLQKEPNLRP ASELLKHPFVTGEEA+SQL S +AC      EPLEI
Sbjct: 301 EQLSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEEAESQLVSRDACT-----EPLEI 360

Query: 392 HSPLYTANLEMSKTSTHLGSSDICNLDSLSCSKVYTGNEQENDIWGTNNSDDEMCQIDDK 451
           HSPLYT+  EMSKT TH GSSDICNLDSLSCSKVYT N+ E+DIW T NSDDEMCQIDDK
Sbjct: 361 HSPLYTSKFEMSKTPTHPGSSDICNLDSLSCSKVYTANKLESDIW-TANSDDEMCQIDDK 420

Query: 452 DDFMLDEVKLRSSIIPENLKSYNPICEPSDDEDCKFDKGPVVDQGSNLDEKALAPGSCSG 511
           DD MLDEVK+ SSI+ ENLKSYNPICEPSDDEDCKFD+ P VDQGSNL+E+ALA  SCSG
Sbjct: 421 DDLMLDEVKIGSSIVHENLKSYNPICEPSDDEDCKFDRSPAVDQGSNLEEEALAARSCSG 480

Query: 512 VFDEDQNFSFPCGRSLSEDEDEVTESKIKAFLDEKAFELKKMQTPLYEEFYNSLNTSCSP 571
           VFDEDQNFSFP GRSLSEDEDE+TESKIKAFL EKA ELKK+Q+PLYEEFYNSLN SCSP
Sbjct: 481 VFDEDQNFSFPRGRSLSEDEDELTESKIKAFLYEKALELKKLQSPLYEEFYNSLNASCSP 540

Query: 572 VFIESKPDESTPKYLKLPPKSRSPSRSPGNQSTVMDAFGTGSPGSGSRSNGNDQRSQLND 631
           VFIESK DESTP YLKLPPKSRSPSRSPGN S  +DAFGTGSP SGSR N NDQRSQL+D
Sbjct: 541 VFIESKRDESTPTYLKLPPKSRSPSRSPGNPSPAIDAFGTGSPASGSRGNANDQRSQLSD 600

Query: 632 WKGLLADGQSEAGSPR-KHYSEIQRKWKEELDQELERKREMMRQAGGGSKTSSPKDKAIG 691
           WKGLL DGQSEAGSP  KHYS+IQRKWKEELDQELE+KREMMRQAGGG KTSSPKDKA+G
Sbjct: 601 WKGLLVDGQSEAGSPSSKHYSDIQRKWKEELDQELEKKREMMRQAGGGGKTSSPKDKAMG 660

Query: 692 RQRERTRFASPLRDDASGTGKENRENIIS 718
           R RERTRFASPLRDDASG G+ENRENIIS
Sbjct: 661 RHRERTRFASPLRDDASGAGRENRENIIS 681

BLAST of MC08g2262 vs. NCBI nr
Match: XP_038877020.1 (mitogen-activated protein kinase kinase kinase NPK1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1178 bits (3048), Expect = 0.0
Identity = 602/690 (87.25%), Postives = 634/690 (91.88%), Query Frame = 0

Query: 32  MQDIFASVRRSLVFRAPLDNEDSHTPPVGVGVGALVDKINSGIRKSRVFSRH-SPSSALP 91
           MQDIFASVRRSLVFR PLDN+DSH+P   +GVGALVDKINS IRKSRVFSRH SPSSALP
Sbjct: 1   MQDIFASVRRSLVFRPPLDNDDSHSP--AIGVGALVDKINSSIRKSRVFSRHYSPSSALP 60

Query: 92  PIPKENEPPIRWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVLIAANGASKEKAQAHIK 151
           PIPK+ +PPIRWRKGELIGCGAFGRVYMGMNL SGELLAVKQVLIAANGASKEKAQAH++
Sbjct: 61  PIPKDTDPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQAHVQ 120

Query: 152 ELEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFEAFPEAVIRTY 211
           ELEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKF AFPEAV+RTY
Sbjct: 121 ELEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFGAFPEAVLRTY 180

Query: 212 TKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVAELATVSGAKSMKGT 271
           TKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQV ELAT+SGAKSMKGT
Sbjct: 181 TKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVVELATISGAKSMKGT 240

Query: 272 PYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSDQYPEVAALFHIGTTKSHPPIP 331
           PYWMAPEVILQTGHSFSADIWSVGCT IEMATGKPPWS QY EVAALFHIGTTKSHPPIP
Sbjct: 241 PYWMAPEVILQTGHSFSADIWSVGCTFIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIP 300

Query: 332 EQLSIEAQDFLLKCLQKEPNLRPVASELLKHPFVTGEEAKSQLASNNACMICHHQEPLEI 391
           EQLS+EA+DFLLKCLQKEPNLRP ASELLKHPFVTGEEA+SQLAS++AC      EPLEI
Sbjct: 301 EQLSVEAKDFLLKCLQKEPNLRPTASELLKHPFVTGEEAESQLASSDACT-----EPLEI 360

Query: 392 HSPLYTANLEMSKTSTHLGSSDICNLDSLSCSKVYTGNEQENDIWGTNNSDDEMCQIDDK 451
            SPLYT+  EMSKT+TH GSSDICNLDSLSCSKVYT N+ E+DIW   NSDDEMCQIDDK
Sbjct: 361 RSPLYTSEHEMSKTATHPGSSDICNLDSLSCSKVYTANKLESDIW-RGNSDDEMCQIDDK 420

Query: 452 DDFMLDEVKLRSSIIPENLKSYNPICEPSDDEDCKFDKGPVVDQGSNLDEKALAPGSCSG 511
           DDFMLDEVK+ SSII EN+KSYNPICEPSDDEDCKFD+ PV+DQGSNL E+ LAPGSCSG
Sbjct: 421 DDFMLDEVKIGSSIIHENMKSYNPICEPSDDEDCKFDRSPVLDQGSNLHEETLAPGSCSG 480

Query: 512 VFDEDQNFSFPCGRSLSEDEDEVTESKIKAFLDEKAFELKKMQTPLYEEFYNSLNTSCSP 571
           V DEDQNFSFP GRS SEDEDE+TESKIKAFLDEKA ELKK+Q+PLYEEFYNSLN SCSP
Sbjct: 481 VLDEDQNFSFPSGRSFSEDEDELTESKIKAFLDEKALELKKLQSPLYEEFYNSLNESCSP 540

Query: 572 VFIESKPDESTPKYLKLPPKSRSPSRSPGNQSTVMDAFGTGSPGSGSRSNGNDQRSQLND 631
           VFIESK +ESTPKYLKLPPKSRSPSRSPGN S  +DAFGT SPGSGSR N NDQRSQLND
Sbjct: 541 VFIESKQNESTPKYLKLPPKSRSPSRSPGNPSPALDAFGTDSPGSGSRGNANDQRSQLND 600

Query: 632 WKGLLADGQSEAGSPRKHYSEIQRKWKEELDQELERKREMMRQAGGGSKTSSPKDKAIGR 691
           WKGLL DGQSE+GSP KHYSEIQRKWKEELDQELERKREMMRQAG G KTSSPKDKA+GR
Sbjct: 601 WKGLLVDGQSESGSPSKHYSEIQRKWKEELDQELERKREMMRQAGAGGKTSSPKDKAMGR 660

Query: 692 QRERTRFASPLRDDASGTG--KENRENIIS 718
            RERTRFASP RDDA+G G  +ENREN IS
Sbjct: 661 PRERTRFASPFRDDATGAGAGRENRENFIS 682

BLAST of MC08g2262 vs. ExPASy TrEMBL
Match: A0A6J1D9F6 (mitogen-activated protein kinase kinase kinase NPK1 OS=Momordica charantia OX=3673 GN=LOC111018841 PE=4 SV=1)

HSP 1 Score: 1346 bits (3483), Expect = 0.0
Identity = 682/687 (99.27%), Postives = 682/687 (99.27%), Query Frame = 0

Query: 32  MQDIFASVRRSLVFRAPLDNEDSHTPPVGVGVGALVDKINSGIRKSRVFSRHSPSSALPP 91
           MQDIFASVRRSLVFRAPLDNEDSHTPPVGVGVGALVDKINSGIRKSRVFSRHSPSSALPP
Sbjct: 1   MQDIFASVRRSLVFRAPLDNEDSHTPPVGVGVGALVDKINSGIRKSRVFSRHSPSSALPP 60

Query: 92  IPKENEPPIRWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVLIAANGASKEKAQAHIKE 151
           IPKENEPPIRWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVLIAANGASKEKAQAHIKE
Sbjct: 61  IPKENEPPIRWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVLIAANGASKEKAQAHIKE 120

Query: 152 LEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFEAFPEAVIRTYT 211
           LEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFEAFPEAVIRTYT
Sbjct: 121 LEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFEAFPEAVIRTYT 180

Query: 212 KQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVAELATVSGAKSMKGTP 271
           KQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVAELATVSGAKSMKGTP
Sbjct: 181 KQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVAELATVSGAKSMKGTP 240

Query: 272 YWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSDQYPEVAALFHIGTTKSHPPIPE 331
           YWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSDQYPEVAALFHIGTTKSHPPIPE
Sbjct: 241 YWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSDQYPEVAALFHIGTTKSHPPIPE 300

Query: 332 QLSIEAQDFLLKCLQKEPNLRPVASELLKHPFVTGEEAKSQLASNNACMICHHQEPLEIH 391
           QLSIEAQDFLLKCLQKEPNLRPVASELLKHPFVTGEEAKSQLASNNACM     EPLEIH
Sbjct: 301 QLSIEAQDFLLKCLQKEPNLRPVASELLKHPFVTGEEAKSQLASNNACM-----EPLEIH 360

Query: 392 SPLYTANLEMSKTSTHLGSSDICNLDSLSCSKVYTGNEQENDIWGTNNSDDEMCQIDDKD 451
           SPLYTANLEMSKTSTHLGSSDICNLDSLSCSKVYTGNEQENDIWGTNNSDDEMCQIDDKD
Sbjct: 361 SPLYTANLEMSKTSTHLGSSDICNLDSLSCSKVYTGNEQENDIWGTNNSDDEMCQIDDKD 420

Query: 452 DFMLDEVKLRSSIIPENLKSYNPICEPSDDEDCKFDKGPVVDQGSNLDEKALAPGSCSGV 511
           DFMLDEVKLRSSIIPENLKSYNPICEPSDDEDCKFDKGPVVDQGSNLDEKALAPGSCSGV
Sbjct: 421 DFMLDEVKLRSSIIPENLKSYNPICEPSDDEDCKFDKGPVVDQGSNLDEKALAPGSCSGV 480

Query: 512 FDEDQNFSFPCGRSLSEDEDEVTESKIKAFLDEKAFELKKMQTPLYEEFYNSLNTSCSPV 571
           FDEDQNFSFPCGRSLSEDEDEVTESKIKAFLDEKAFELKKMQTPLYEEFYNSLNTSCSPV
Sbjct: 481 FDEDQNFSFPCGRSLSEDEDEVTESKIKAFLDEKAFELKKMQTPLYEEFYNSLNTSCSPV 540

Query: 572 FIESKPDESTPKYLKLPPKSRSPSRSPGNQSTVMDAFGTGSPGSGSRSNGNDQRSQLNDW 631
           FIESKPDESTPKYLKLPPKSRSPSRSPGNQSTVMDAFGTGSPGSGSRSNGNDQRSQLNDW
Sbjct: 541 FIESKPDESTPKYLKLPPKSRSPSRSPGNQSTVMDAFGTGSPGSGSRSNGNDQRSQLNDW 600

Query: 632 KGLLADGQSEAGSPRKHYSEIQRKWKEELDQELERKREMMRQAGGGSKTSSPKDKAIGRQ 691
           KGLLADGQSEAGSPRKHYSEIQRKWKEELDQELERKREMMRQAGGGSKTSSPKDKAIGRQ
Sbjct: 601 KGLLADGQSEAGSPRKHYSEIQRKWKEELDQELERKREMMRQAGGGSKTSSPKDKAIGRQ 660

Query: 692 RERTRFASPLRDDASGTGKENRENIIS 718
           RERTRFASPLRDDASGTGKENRENIIS
Sbjct: 661 RERTRFASPLRDDASGTGKENRENIIS 682

BLAST of MC08g2262 vs. ExPASy TrEMBL
Match: A0A6J1G491 (mitogen-activated protein kinase kinase kinase NPK1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111450592 PE=4 SV=1)

HSP 1 Score: 1184 bits (3062), Expect = 0.0
Identity = 605/688 (87.94%), Postives = 633/688 (92.01%), Query Frame = 0

Query: 32  MQDIFASVRRSLVFRAPLDNEDSHTPPVGVGVGALVDKINSGIRKSRVFSRHSPSS-ALP 91
           MQDIFASVRRSLVFRAPL+NED H+P   +GVGALVDKINS IRKSRVFSRHSPSS ALP
Sbjct: 1   MQDIFASVRRSLVFRAPLENEDPHSP--AIGVGALVDKINSSIRKSRVFSRHSPSSSALP 60

Query: 92  PIPKENEPPIRWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVLIAANGASKEKAQAHIK 151
           PIPKE +PPIRWRKGELIGCGAFGRVYMGMNL SGELLAVKQVLIAANGASKEKAQ HIK
Sbjct: 61  PIPKETDPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQTHIK 120

Query: 152 ELEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFEAFPEAVIRTY 211
           ELEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKF AFPEAV+RTY
Sbjct: 121 ELEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFGAFPEAVLRTY 180

Query: 212 TKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVAELATVSGAKSMKGT 271
           TKQLLLGLEYLHKNGIMHRDIKGANILVDN GCIKLADFGASKQV ELAT+SGAKSMKGT
Sbjct: 181 TKQLLLGLEYLHKNGIMHRDIKGANILVDNNGCIKLADFGASKQVVELATISGAKSMKGT 240

Query: 272 PYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSDQYPEVAALFHIGTTKSHPPIP 331
           PYWMAPEVILQTGH+FSADIWSVGCTVIEMATGKPPWS QY EVAALFHIGTTKSHPPIP
Sbjct: 241 PYWMAPEVILQTGHNFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIP 300

Query: 332 EQLSIEAQDFLLKCLQKEPNLRPVASELLKHPFVTGEEAKSQLASNNACMICHHQEPLEI 391
           EQLS+EA+DFLLKCLQKEPNLRP ASELLKHPFVTGEEA+SQL S +AC      EPLEI
Sbjct: 301 EQLSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEEAESQLVSRDACT-----EPLEI 360

Query: 392 HSPLYTANLEMSKTSTHLGSSDICNLDSLSCSKVYTGNEQENDIWGTNNSDDEMCQIDDK 451
           HSPLYT+  EMSKT TH GSSDICNLDSLSCSKVYT N+ E+DIW T NSDDEMCQIDDK
Sbjct: 361 HSPLYTSKFEMSKTPTHPGSSDICNLDSLSCSKVYTANKLESDIW-TANSDDEMCQIDDK 420

Query: 452 DDFMLDEVKLRSSIIPENLKSYNPICEPSDDEDCKFDKGPVVDQGSNLDEKALAPGSCSG 511
           DD MLDEVK+ SSI+ ENLKSYNPICEPSDDEDCKFD+ P VDQGSNL+E+ALA  SCSG
Sbjct: 421 DDLMLDEVKIGSSIVHENLKSYNPICEPSDDEDCKFDRSPAVDQGSNLEEEALAARSCSG 480

Query: 512 VFDEDQNFSFPCGRSLSEDEDEVTESKIKAFLDEKAFELKKMQTPLYEEFYNSLNTSCSP 571
           VFDEDQNFSFP GRSLSEDEDE+TESKIKAFL EKA ELKK+Q+PLYEEFYNSLN SCSP
Sbjct: 481 VFDEDQNFSFPRGRSLSEDEDELTESKIKAFLYEKALELKKLQSPLYEEFYNSLNASCSP 540

Query: 572 VFIESKPDESTPKYLKLPPKSRSPSRSPGNQSTVMDAFGTGSPGSGSRSNGNDQRSQLND 631
           VFIESK DESTP YLKLPPKSRSPSRSPGN S  +DAFGTGSP SGSR N NDQRSQL+D
Sbjct: 541 VFIESKRDESTPTYLKLPPKSRSPSRSPGNPSPAIDAFGTGSPASGSRGNANDQRSQLSD 600

Query: 632 WKGLLADGQSEAGSPRKHYSEIQRKWKEELDQELERKREMMRQAGGGSKTSSPKDKAIGR 691
           WKGLL DGQSEAGSP KHYS+IQRKWKEELDQELE+KREMMRQAGGG KTSSPKDKA+GR
Sbjct: 601 WKGLLVDGQSEAGSPSKHYSDIQRKWKEELDQELEKKREMMRQAGGGGKTSSPKDKAMGR 660

Query: 692 QRERTRFASPLRDDASGTGKENRENIIS 718
            RERTRFASPLRDDASG G+ENRENIIS
Sbjct: 661 HRERTRFASPLRDDASGAGRENRENIIS 680

BLAST of MC08g2262 vs. ExPASy TrEMBL
Match: A0A6J1G469 (mitogen-activated protein kinase kinase kinase NPK1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450592 PE=4 SV=1)

HSP 1 Score: 1179 bits (3050), Expect = 0.0
Identity = 605/689 (87.81%), Postives = 633/689 (91.87%), Query Frame = 0

Query: 32  MQDIFASVRRSLVFRAPLDNEDSHTPPVGVGVGALVDKINSGIRKSRVFSRHSPSS-ALP 91
           MQDIFASVRRSLVFRAPL+NED H+P   +GVGALVDKINS IRKSRVFSRHSPSS ALP
Sbjct: 1   MQDIFASVRRSLVFRAPLENEDPHSP--AIGVGALVDKINSSIRKSRVFSRHSPSSSALP 60

Query: 92  PIPKENEPPIRWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVLIAANGASKEKAQAHIK 151
           PIPKE +PPIRWRKGELIGCGAFGRVYMGMNL SGELLAVKQVLIAANGASKEKAQ HIK
Sbjct: 61  PIPKETDPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQTHIK 120

Query: 152 ELEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFEAFPEAVIRTY 211
           ELEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKF AFPEAV+RTY
Sbjct: 121 ELEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFGAFPEAVLRTY 180

Query: 212 TKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVAELATVSGAKSMKGT 271
           TKQLLLGLEYLHKNGIMHRDIKGANILVDN GCIKLADFGASKQV ELAT+SGAKSMKGT
Sbjct: 181 TKQLLLGLEYLHKNGIMHRDIKGANILVDNNGCIKLADFGASKQVVELATISGAKSMKGT 240

Query: 272 PYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSDQYPEVAALFHIGTTKSHPPIP 331
           PYWMAPEVILQTGH+FSADIWSVGCTVIEMATGKPPWS QY EVAALFHIGTTKSHPPIP
Sbjct: 241 PYWMAPEVILQTGHNFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIP 300

Query: 332 EQLSIEAQDFLLKCLQKEPNLRPVASELLKHPFVTGEEAKSQLASNNACMICHHQEPLEI 391
           EQLS+EA+DFLLKCLQKEPNLRP ASELLKHPFVTGEEA+SQL S +AC      EPLEI
Sbjct: 301 EQLSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEEAESQLVSRDACT-----EPLEI 360

Query: 392 HSPLYTANLEMSKTSTHLGSSDICNLDSLSCSKVYTGNEQENDIWGTNNSDDEMCQIDDK 451
           HSPLYT+  EMSKT TH GSSDICNLDSLSCSKVYT N+ E+DIW T NSDDEMCQIDDK
Sbjct: 361 HSPLYTSKFEMSKTPTHPGSSDICNLDSLSCSKVYTANKLESDIW-TANSDDEMCQIDDK 420

Query: 452 DDFMLDEVKLRSSIIPENLKSYNPICEPSDDEDCKFDKGPVVDQGSNLDEKALAPGSCSG 511
           DD MLDEVK+ SSI+ ENLKSYNPICEPSDDEDCKFD+ P VDQGSNL+E+ALA  SCSG
Sbjct: 421 DDLMLDEVKIGSSIVHENLKSYNPICEPSDDEDCKFDRSPAVDQGSNLEEEALAARSCSG 480

Query: 512 VFDEDQNFSFPCGRSLSEDEDEVTESKIKAFLDEKAFELKKMQTPLYEEFYNSLNTSCSP 571
           VFDEDQNFSFP GRSLSEDEDE+TESKIKAFL EKA ELKK+Q+PLYEEFYNSLN SCSP
Sbjct: 481 VFDEDQNFSFPRGRSLSEDEDELTESKIKAFLYEKALELKKLQSPLYEEFYNSLNASCSP 540

Query: 572 VFIESKPDESTPKYLKLPPKSRSPSRSPGNQSTVMDAFGTGSPGSGSRSNGNDQRSQLND 631
           VFIESK DESTP YLKLPPKSRSPSRSPGN S  +DAFGTGSP SGSR N NDQRSQL+D
Sbjct: 541 VFIESKRDESTPTYLKLPPKSRSPSRSPGNPSPAIDAFGTGSPASGSRGNANDQRSQLSD 600

Query: 632 WKGLLADGQSEAGSPR-KHYSEIQRKWKEELDQELERKREMMRQAGGGSKTSSPKDKAIG 691
           WKGLL DGQSEAGSP  KHYS+IQRKWKEELDQELE+KREMMRQAGGG KTSSPKDKA+G
Sbjct: 601 WKGLLVDGQSEAGSPSSKHYSDIQRKWKEELDQELEKKREMMRQAGGGGKTSSPKDKAMG 660

Query: 692 RQRERTRFASPLRDDASGTGKENRENIIS 718
           R RERTRFASPLRDDASG G+ENRENIIS
Sbjct: 661 RHRERTRFASPLRDDASGAGRENRENIIS 681

BLAST of MC08g2262 vs. ExPASy TrEMBL
Match: A0A6J1KCT1 (mitogen-activated protein kinase kinase kinase NPK1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493747 PE=4 SV=1)

HSP 1 Score: 1172 bits (3033), Expect = 0.0
Identity = 600/689 (87.08%), Postives = 631/689 (91.58%), Query Frame = 0

Query: 32  MQDIFASVRRSLVFRAPLDNEDSHTPPVGVGVGALVDKINSGIRKSRVFSRHSPSS-ALP 91
           MQDIFASVRRSLVFRAPL+NED H+P   +GVGALVDKINS IRKSRVFSRHSPSS ALP
Sbjct: 1   MQDIFASVRRSLVFRAPLENEDPHSP--AIGVGALVDKINSSIRKSRVFSRHSPSSSALP 60

Query: 92  PIPKENEPPIRWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVLIAANGASKEKAQAHIK 151
           PIPKE +PPIRWRKGELIGCGAFGRVYMGMNL SGELLAVKQVLIAANGASKEKAQ H+K
Sbjct: 61  PIPKETDPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQTHVK 120

Query: 152 ELEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFEAFPEAVIRTY 211
           ELEEEVKLLKDLSH NIVRYLGTVREDDSLNILLEFVPGGSISSLLGKF AFPEAV+RTY
Sbjct: 121 ELEEEVKLLKDLSHQNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFGAFPEAVLRTY 180

Query: 212 TKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVAELATVSGAKSMKGT 271
           TKQLLLGLEYLHKNGIMHRDIKGANILVDN GCIKLADFGASKQV ELAT+SGAKSMKGT
Sbjct: 181 TKQLLLGLEYLHKNGIMHRDIKGANILVDNNGCIKLADFGASKQVVELATISGAKSMKGT 240

Query: 272 PYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSDQYPEVAALFHIGTTKSHPPIP 331
           PYWMAPEVILQTGH+FSADIWSVGCTVIEMATGKPPWS QY EVAALFHIGTTKSHPPIP
Sbjct: 241 PYWMAPEVILQTGHNFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIP 300

Query: 332 EQLSIEAQDFLLKCLQKEPNLRPVASELLKHPFVTGEEAKSQLASNNACMICHHQEPLEI 391
           EQLS+EA+DFLLKCLQKEPNLRP ASELLKHPFVTGEE +SQ+ S +AC      EPLEI
Sbjct: 301 EQLSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEEVESQIVSRDACT-----EPLEI 360

Query: 392 HSPLYTANLEMSKTSTHLGSSDICNLDSLSCSKVYTGNEQENDIWGTNNSDDEMCQIDDK 451
           HSPLYT+N EMSKT TH GSSDICNLDSLSCSKVYT N+ E+ IW  N SDDEMCQIDDK
Sbjct: 361 HSPLYTSNFEMSKTPTHPGSSDICNLDSLSCSKVYTANKLESGIWAAN-SDDEMCQIDDK 420

Query: 452 DDFMLDEVKLRSSIIPENLKSYNPICEPSDDEDCKFDKGPVVDQGSNLDEKALAPGSCSG 511
           DD MLDEVK+ SSI+ ENLKSYNPICEPSDDEDCKFD+ P VDQGSNL+E+ALA  SCSG
Sbjct: 421 DDLMLDEVKIGSSIVHENLKSYNPICEPSDDEDCKFDRSPAVDQGSNLEEEALAARSCSG 480

Query: 512 VFDEDQNFSFPCGRSLSEDEDEVTESKIKAFLDEKAFELKKMQTPLYEEFYNSLNTSCSP 571
           VFDEDQNFSFP GRSLSEDEDE+TESKIKAFL EKA ELKK+Q+PLYEEFYNSLN SCSP
Sbjct: 481 VFDEDQNFSFPRGRSLSEDEDELTESKIKAFLYEKALELKKLQSPLYEEFYNSLNASCSP 540

Query: 572 VFIESKPDESTPKYLKLPPKSRSPSRSPGNQSTVMDAFGTGSPGSGS-RSNGNDQRSQLN 631
           VFIESK DESTP YLKLPPKSRSPSRSPGN S  +DAFGTGSP SGS R N NDQRSQL+
Sbjct: 541 VFIESKRDESTPTYLKLPPKSRSPSRSPGNPSPAIDAFGTGSPASGSSRGNANDQRSQLS 600

Query: 632 DWKGLLADGQSEAGSPRKHYSEIQRKWKEELDQELERKREMMRQAGGGSKTSSPKDKAIG 691
           DWKGLL +GQSEAGSP KHYS+IQRKWKEELDQELE+KREMMRQAGGG KTSSPKDKA+G
Sbjct: 601 DWKGLLVEGQSEAGSPSKHYSDIQRKWKEELDQELEKKREMMRQAGGGGKTSSPKDKAMG 660

Query: 692 RQRERTRFASPLRDDASGTGKENRENIIS 718
           RQRERTRFASPLRDDASG G+ENRENIIS
Sbjct: 661 RQRERTRFASPLRDDASGAGRENRENIIS 681

BLAST of MC08g2262 vs. ExPASy TrEMBL
Match: A0A6J1KF48 (mitogen-activated protein kinase kinase kinase NPK1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493747 PE=4 SV=1)

HSP 1 Score: 1168 bits (3021), Expect = 0.0
Identity = 600/690 (86.96%), Postives = 631/690 (91.45%), Query Frame = 0

Query: 32  MQDIFASVRRSLVFRAPLDNEDSHTPPVGVGVGALVDKINSGIRKSRVFSRHSPSS-ALP 91
           MQDIFASVRRSLVFRAPL+NED H+P   +GVGALVDKINS IRKSRVFSRHSPSS ALP
Sbjct: 1   MQDIFASVRRSLVFRAPLENEDPHSP--AIGVGALVDKINSSIRKSRVFSRHSPSSSALP 60

Query: 92  PIPKENEPPIRWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVLIAANGASKEKAQAHIK 151
           PIPKE +PPIRWRKGELIGCGAFGRVYMGMNL SGELLAVKQVLIAANGASKEKAQ H+K
Sbjct: 61  PIPKETDPPIRWRKGELIGCGAFGRVYMGMNLGSGELLAVKQVLIAANGASKEKAQTHVK 120

Query: 152 ELEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFEAFPEAVIRTY 211
           ELEEEVKLLKDLSH NIVRYLGTVREDDSLNILLEFVPGGSISSLLGKF AFPEAV+RTY
Sbjct: 121 ELEEEVKLLKDLSHQNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFGAFPEAVLRTY 180

Query: 212 TKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVAELATVSGAKSMKGT 271
           TKQLLLGLEYLHKNGIMHRDIKGANILVDN GCIKLADFGASKQV ELAT+SGAKSMKGT
Sbjct: 181 TKQLLLGLEYLHKNGIMHRDIKGANILVDNNGCIKLADFGASKQVVELATISGAKSMKGT 240

Query: 272 PYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSDQYPEVAALFHIGTTKSHPPIP 331
           PYWMAPEVILQTGH+FSADIWSVGCTVIEMATGKPPWS QY EVAALFHIGTTKSHPPIP
Sbjct: 241 PYWMAPEVILQTGHNFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIP 300

Query: 332 EQLSIEAQDFLLKCLQKEPNLRPVASELLKHPFVTGEEAKSQLASNNACMICHHQEPLEI 391
           EQLS+EA+DFLLKCLQKEPNLRP ASELLKHPFVTGEE +SQ+ S +AC      EPLEI
Sbjct: 301 EQLSVEAKDFLLKCLQKEPNLRPAASELLKHPFVTGEEVESQIVSRDACT-----EPLEI 360

Query: 392 HSPLYTANLEMSKTSTHLGSSDICNLDSLSCSKVYTGNEQENDIWGTNNSDDEMCQIDDK 451
           HSPLYT+N EMSKT TH GSSDICNLDSLSCSKVYT N+ E+ IW  N SDDEMCQIDDK
Sbjct: 361 HSPLYTSNFEMSKTPTHPGSSDICNLDSLSCSKVYTANKLESGIWAAN-SDDEMCQIDDK 420

Query: 452 DDFMLDEVKLRSSIIPENLKSYNPICEPSDDEDCKFDKGPVVDQGSNLDEKALAPGSCSG 511
           DD MLDEVK+ SSI+ ENLKSYNPICEPSDDEDCKFD+ P VDQGSNL+E+ALA  SCSG
Sbjct: 421 DDLMLDEVKIGSSIVHENLKSYNPICEPSDDEDCKFDRSPAVDQGSNLEEEALAARSCSG 480

Query: 512 VFDEDQNFSFPCGRSLSEDEDEVTESKIKAFLDEKAFELKKMQTPLYEEFYNSLNTSCSP 571
           VFDEDQNFSFP GRSLSEDEDE+TESKIKAFL EKA ELKK+Q+PLYEEFYNSLN SCSP
Sbjct: 481 VFDEDQNFSFPRGRSLSEDEDELTESKIKAFLYEKALELKKLQSPLYEEFYNSLNASCSP 540

Query: 572 VFIESKPDESTPKYLKLPPKSRSPSRSPGNQSTVMDAFGTGSPGSGS-RSNGNDQRSQLN 631
           VFIESK DESTP YLKLPPKSRSPSRSPGN S  +DAFGTGSP SGS R N NDQRSQL+
Sbjct: 541 VFIESKRDESTPTYLKLPPKSRSPSRSPGNPSPAIDAFGTGSPASGSSRGNANDQRSQLS 600

Query: 632 DWKGLLADGQSEAGSPR-KHYSEIQRKWKEELDQELERKREMMRQAGGGSKTSSPKDKAI 691
           DWKGLL +GQSEAGSP  KHYS+IQRKWKEELDQELE+KREMMRQAGGG KTSSPKDKA+
Sbjct: 601 DWKGLLVEGQSEAGSPSSKHYSDIQRKWKEELDQELEKKREMMRQAGGGGKTSSPKDKAM 660

Query: 692 GRQRERTRFASPLRDDASGTGKENRENIIS 718
           GRQRERTRFASPLRDDASG G+ENRENIIS
Sbjct: 661 GRQRERTRFASPLRDDASGAGRENRENIIS 682

BLAST of MC08g2262 vs. TAIR 10
Match: AT1G09000.1 (NPK1-related protein kinase 1 )

HSP 1 Score: 686.4 bits (1770), Expect = 2.4e-197
Identity = 413/696 (59.34%), Postives = 493/696 (70.83%), Query Frame = 0

Query: 32  MQDIFASVRRSLVFRAPLDNEDSHTPPVGVGVGALVDKINSGIRKSRVFSRHSPSSALPP 91
           MQD F SVRRSLVFR   D+++    P   GV  L DKI S IRKS++F + S S   P 
Sbjct: 1   MQDFFGSVRRSLVFRPSSDDDNQENQPPFPGV--LADKITSCIRKSKIFIKPSFSPPPPA 60

Query: 92  IPKENEPPIRWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVLIAANGASKEKAQAHIKE 151
              +  PPI WRKG+LIG GAFG VYMGMNLDSGELLAVKQVLIAAN ASKEK QAHI+E
Sbjct: 61  NTVDMAPPISWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQE 120

Query: 152 LEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFEAFPEAVIRTYT 211
           LEEEVKLLK+LSHPNIVRYLGTVREDD+LNILLEFVPGGSISSLL KF  FPE+V+RTYT
Sbjct: 121 LEEEVKLLKNLSHPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYT 180

Query: 212 KQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVAELATVSGAKSMKGTP 271
           +QLLLGLEYLH + IMHRDIKGANILVDNKGCIKLADFGASKQVAELAT++GAKSMKGTP
Sbjct: 181 RQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTP 240

Query: 272 YWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSDQYPEVAALFHIGTTKSHPPIPE 331
           YWMAPEVILQTGHSFSADIWSVGCTVIEM TGK PWS QY EVAA+F IGTTKSHPPIP+
Sbjct: 241 YWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPD 300

Query: 332 QLSIEAQDFLLKCLQKEPNLRPVASELLKHPFVTGEEAKSQLASNNACMICHHQEPLEIH 391
            LS +A+DFLLKCLQ+ PNLRP ASELLKHPFV G+  +S  AS +   + ++   L   
Sbjct: 301 TLSSDAKDFLLKCLQEVPNLRPTASELLKHPFVMGKHKES--ASTDLGSVLNN---LSTP 360

Query: 392 SPLYTANLEMSKTSTHLGSSDICNLDSLSCSKV-YTGNEQENDIWGTNNS---DDEMCQI 451
            PL   N + +  ST     D+CN  SL+ S V    + Q  ++W  N++   +D+MC I
Sbjct: 361 LPLQINNTKSTPDSTCDDVGDMCNFGSLNYSLVDPVKSIQNKNLWQQNDNGGDEDDMCLI 420

Query: 452 DDKDDFMLDEVKLRSSIIPE--NLKSYNPICEPSDDEDCKFDKGPVVDQGSNLDEKALAP 511
           DD ++F+  + ++ S++  +    KS + I + S     KFD+ P               
Sbjct: 421 DD-ENFLTFDGEMSSTLEKDCHLKKSCDDISDMSIALKSKFDESP--------------- 480

Query: 512 GSCSGVFDEDQNFSFPCGR-SLSEDEDEVTESKIKAFLDEKAFELKKMQTPLYEEFYNSL 571
               G  +++   S  C + S SED+DE+TESKIKAFLDEKA +LKK+QTPLYEEFYNSL
Sbjct: 481 ----GNGEKESTMSMECDQPSYSEDDDELTESKIKAFLDEKAADLKKLQTPLYEEFYNSL 540

Query: 572 NTSCSPVFIE-----SKPDESTPKYLKLPPKSRSPSRSP--GNQSTVMDAFG-TGSPGSG 631
            T  SP  +E     SK +++   +LKLPPKSRSPSR P  G+ S   DA   + SPGSG
Sbjct: 541 IT-FSPSCMESNLSNSKREDTARGFLKLPPKSRSPSRGPLGGSPSRATDATSCSKSPGSG 600

Query: 632 SR-----SNGNDQRSQ------LNDWKGLLADGQSEAGSPRKHYSEIQRKWKEELDQELE 691
                  +NG D+ SQ      + DW+GL+ D + E  S     SEI++KWKEELDQELE
Sbjct: 601 GSRELNINNGGDEASQDGVSARVTDWRGLVVDTKQEL-SQCVALSEIEKKWKEELDQELE 660

Query: 692 RKR-EMMRQAGGGSKTSSPKDKAIGRQRERTRFASP 701
           RKR E+MRQAG G   SSP+D+ + RQRE++RFASP
Sbjct: 661 RKRQEIMRQAGLG---SSPRDRGMSRQREKSRFASP 664

BLAST of MC08g2262 vs. TAIR 10
Match: AT3G06030.1 (NPK1-related protein kinase 3 )

HSP 1 Score: 634.8 bits (1636), Expect = 8.4e-182
Identity = 361/655 (55.11%), Postives = 449/655 (68.55%), Query Frame = 0

Query: 32  MQDIFASVRRSLVFRAPLDNEDSHTPPVGVGVGALVDKINSGIRKSRVFSRHSPSSALPP 91
           MQDI  SVRRSLVFR+ L  +D  +   G G+   V KINS IR SR+     P   LP 
Sbjct: 1   MQDILGSVRRSLVFRSSLAGDDGTS---GGGLSGFVGKINSSIRSSRIGLFSKPPPGLPA 60

Query: 92  IPKENEPPIRWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVLIAANGASKEKAQAHIKE 151
             KE  P IRWRKGELIGCGAFGRVYMGMNLDSGELLA+KQVLIA + ASKEK Q HI+E
Sbjct: 61  PRKEEAPSIRWRKGELIGCGAFGRVYMGMNLDSGELLAIKQVLIAPSSASKEKTQGHIRE 120

Query: 152 LEEEVKLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFEAFPEAVIRTYT 211
           LEEEV+LLK+LSHPNIVRYLGTVRE DSLNIL+EFVPGGSISSLL KF +FPE VI  YT
Sbjct: 121 LEEEVQLLKNLSHPNIVRYLGTVRESDSLNILMEFVPGGSISSLLEKFGSFPEPVIIMYT 180

Query: 212 KQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVAELATVSGAKSMKGTP 271
           KQLLLGLEYLH NGIMHRDIKGANILVDNKGCI+LADFGASK+V ELATV+GAKSMKGTP
Sbjct: 181 KQLLLGLEYLHNNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATVNGAKSMKGTP 240

Query: 272 YWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSDQYPEVAALFHIGTTKSHPPIPE 331
           YWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWS+QY + AA+ HIG TK+HPPIPE
Sbjct: 241 YWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTKAHPPIPE 300

Query: 332 QLSIEAQDFLLKCLQKEPNLRPVASELLKHPFVTGEEAKSQLASNNACMICHHQEPLEIH 391
            LS EA+DFL+KCL KEP+LR  A+ELL+HPFVTG+  +   A  N+   C +    +  
Sbjct: 301 DLSPEAKDFLMKCLHKEPSLRLSATELLQHPFVTGKRQEPYPAYRNSLTECGNPITTQGM 360

Query: 392 SPLYTANLEMSKTSTHLGSSDICNLDSLSCSKVYTGNEQENDIWGTNNSDDEMCQIDDKD 451
           +   + N  + + ST  G  D+C L SL  S +Y      +     +   D++CQ D  D
Sbjct: 361 NVRSSIN-SLIRRSTCSGLKDVCELGSLRSSIIYPQKSNNSGFGWRDGDSDDLCQTDMDD 420

Query: 452 DFMLDEVKLRSSIIPENL---KSYNPICEPSDDEDCKFDKGP-VVDQGSNLDEKALAPGS 511
              ++ V  R++++ ++    KS+NP+C+ +D+  CKFD+ P V+   SNL     +   
Sbjct: 421 LCNIESV--RNNVLSQSTDLNKSFNPMCDSTDNWSCKFDESPKVMKSKSNLLSYQASQLQ 480

Query: 512 CSGVFDEDQNFSFPCGRSLSEDEDEVTESKIKAFLDEKAFELKKMQTPLYEEFYNSLNTS 571
                DE+ + +F  G S++ED+ + TE KIK+FLDEKA +LK++QTPL EEF+N++N  
Sbjct: 481 TGVPCDEETSLTFAGGSSVAEDDYKGTELKIKSFLDEKAQDLKRLQTPLLEEFHNAMNPG 540

Query: 572 CSPVFIESKPDESTPKYLKLPPKSRSPSRSPGNQ-STVMDAFGTGSPGSGSRSNGNDQRS 631
                +      + P    LP  S++P R P  + S + DA    SP   S+   N  R 
Sbjct: 541 IPQGALGDTNIYNLP---NLPSISKTPKRLPSRRLSAISDAM--PSPLKSSKRTLNTSRV 600

Query: 632 QLNDWKGLLADGQSEAG-SPRKHYSEIQRKWKEELDQELERKREMMRQAGGGSKT 681
             +  +    +  ++ G +  + +SEI+RKW+EEL +ELER RE +R AG G KT
Sbjct: 601 MQSGTEPTQVNESTKKGVNNSRCFSEIRRKWEEELYEELERHRENLRHAGAGGKT 644

BLAST of MC08g2262 vs. TAIR 10
Match: AT1G54960.1 (NPK1-related protein kinase 2 )

HSP 1 Score: 632.1 bits (1629), Expect = 5.5e-181
Identity = 379/630 (60.16%), Postives = 446/630 (70.79%), Query Frame = 0

Query: 97  EPPIRWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVLIAANGASKEKAQAHIKELEEEV 156
           +PPIRWRKG+LIG GAFG VYMGMNLDSGELLAVKQVLI +N ASKEK QAHI+ELEEEV
Sbjct: 18  KPPIRWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCASKEKTQAHIQELEEEV 77

Query: 157 KLLKDLSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFEAFPEAVIRTYTKQLLL 216
           KLLK+LSHPNIVRYLGTVRED++LNILLEFVPGGSISSLL KF AFPE+V+RTYT QLLL
Sbjct: 78  KLLKNLSHPNIVRYLGTVREDETLNILLEFVPGGSISSLLEKFGAFPESVVRTYTNQLLL 137

Query: 217 GLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKQVAELATVSGAKSMKGTPYWMAP 276
           GLEYLH + IMHRDIKGANILVDN+GCIKLADFGASKQVAELAT+SGAKSMKGTPYWMAP
Sbjct: 138 GLEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATISGAKSMKGTPYWMAP 197

Query: 277 EVILQTGHSFSADIWSVGCTVIEMATGKPPWSDQYPEVAALFHIGTTKSHPPIPEQLSIE 336
           EVILQTGHSFSADIWSVGCTVIEM TGK PWS QY E+AA+FHIGTTKSHPPIP+ +S +
Sbjct: 198 EVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKSHPPIPDNISSD 257

Query: 337 AQDFLLKCLQKEPNLRPVASELLKHPFVTG---EEAKSQLAS--NNACMICHHQEPLEIH 396
           A DFLLKCLQ+EPNLRP ASELLKHPFVTG   E A   L S  +N+C       PL   
Sbjct: 258 ANDFLLKCLQQEPNLRPTASELLKHPFVTGKQKESASKDLTSFMDNSC------SPL--- 317

Query: 397 SPLYTANLEMSKTSTHLGSSDICNLDSLSCSKVYTGNEQEND--IWGTNN-----SDDEM 456
            P    N+   +TST     DICNL SL+C+  +     +N+     +NN      D++M
Sbjct: 318 -PSELTNITSYQTSTSDDVGDICNLGSLTCTLAFPEKSIQNNSLCLKSNNGYDDDDDNDM 377

Query: 457 CQIDDKDDFMLDEVKLRSSI--IPENLKSYNPICEPSDDEDCKFDKGPVVDQGSNLDEKA 516
           C IDD ++F+    +   S+    +  KS + + E SD   CKFD+              
Sbjct: 378 CLIDD-ENFLTYNGETGPSLDNNTDAKKSCDTMSEISDILKCKFDEN------------- 437

Query: 517 LAPGSCSGVFDEDQNFSFPCGR-SLSEDEDEVTESKIKAFLDEKAFELKKMQTPLYEEFY 576
                 SG  + +   S      S SEDE+E+TESKIKAFLD+KA ELKK+QTPLYEEFY
Sbjct: 438 ------SGNGETETKVSMEVDHPSYSEDENELTESKIKAFLDDKAAELKKLQTPLYEEFY 497

Query: 577 NSLNTSCSPVFIES-----KPDESTPKYLKLPPKSRSPS-----RSPGNQSTVMDAFGTG 636
           N + T CSP+ +ES     K +E+   +LKLPPKSRSPS     RSP   +    A  + 
Sbjct: 498 NGMIT-CSPICMESNINNNKREEAPRGFLKLPPKSRSPSQGHIGRSPSRATDA--ACCSK 557

Query: 637 SPGSGSRSNGNDQRSQLNDWKGLLADGQSEAGSPRKHYSEIQRKWKEELDQELERK-REM 696
           SP SG+ S+G  + S  +      A  + E+ S     SEI+RKWKEELDQELERK RE+
Sbjct: 558 SPESGN-SSGAPKNSNAS------AGAEQESNSQSVALSEIERKWKEELDQELERKRREI 604

Query: 697 MRQAGGGSKTSSPKDKAIGRQRERTRFASP 701
            RQAG G   SSP+D+++ R RE++RFASP
Sbjct: 618 TRQAGMG---SSPRDRSLSRHREKSRFASP 604

BLAST of MC08g2262 vs. TAIR 10
Match: AT1G53570.1 (mitogen-activated protein kinase kinase kinase 3 )

HSP 1 Score: 278.1 bits (710), Expect = 2.0e-74
Identity = 131/264 (49.62%), Postives = 190/264 (71.97%), Query Frame = 0

Query: 102 WRKGELIGCGAFGRVYMGMNLDSGELLAVKQVLIAANGASKEKAQAHIKELEEEVKLLKD 161
           W+KG+ +G G FG+VY+G N + G++ A+K+V + ++  + ++    +K+L +E+ LL  
Sbjct: 214 WKKGKFLGSGTFGQVYLGFNSEKGKMCAIKEVKVISDDQTSKEC---LKQLNQEINLLNQ 273

Query: 162 LSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFEAFPEAVIRTYTKQLLLGLEYL 221
           L HPNIV+Y G+   +++L++ LE+V GGSI  LL  + +F E VI+ YT+Q+L GL YL
Sbjct: 274 LCHPNIVQYYGSELSEETLSVYLEYVSGGSIHKLLKDYGSFTEPVIQNYTRQILAGLAYL 333

Query: 222 HKNGIMHRDIKGANILVDNKGCIKLADFGASKQVAELATVSGAKSMKGTPYWMAPEVIL- 281
           H    +HRDIKGANILVD  G IKLADFG +K V   +T+    S KG+PYWMAPEV++ 
Sbjct: 334 HGRNTVHRDIKGANILVDPNGEIKLADFGMAKHVTAFSTM---LSFKGSPYWMAPEVVMS 393

Query: 282 QTGHSFSADIWSVGCTVIEMATGKPPWSDQYPEVAALFHIGTTKSHPPIPEQLSIEAQDF 341
           Q G++ + DIWS+GCT++EMAT KPPWS Q+  VAA+F IG +K  P IP+ LS +A++F
Sbjct: 394 QNGYTHAVDIWSLGCTILEMATSKPPWS-QFEGVAAIFKIGNSKDTPEIPDHLSNDAKNF 453

Query: 342 LLKCLQKEPNLRPVASELLKHPFV 365
           +  CLQ+ P +RP AS+LL+HPF+
Sbjct: 454 IRLCLQRNPTVRPTASQLLEHPFL 470

BLAST of MC08g2262 vs. TAIR 10
Match: AT1G53570.2 (mitogen-activated protein kinase kinase kinase 3 )

HSP 1 Score: 278.1 bits (710), Expect = 2.0e-74
Identity = 131/264 (49.62%), Postives = 190/264 (71.97%), Query Frame = 0

Query: 102 WRKGELIGCGAFGRVYMGMNLDSGELLAVKQVLIAANGASKEKAQAHIKELEEEVKLLKD 161
           W+KG+ +G G FG+VY+G N + G++ A+K+V + ++  + ++    +K+L +E+ LL  
Sbjct: 214 WKKGKFLGSGTFGQVYLGFNSEKGKMCAIKEVKVISDDQTSKEC---LKQLNQEINLLNQ 273

Query: 162 LSHPNIVRYLGTVREDDSLNILLEFVPGGSISSLLGKFEAFPEAVIRTYTKQLLLGLEYL 221
           L HPNIV+Y G+   +++L++ LE+V GGSI  LL  + +F E VI+ YT+Q+L GL YL
Sbjct: 274 LCHPNIVQYYGSELSEETLSVYLEYVSGGSIHKLLKDYGSFTEPVIQNYTRQILAGLAYL 333

Query: 222 HKNGIMHRDIKGANILVDNKGCIKLADFGASKQVAELATVSGAKSMKGTPYWMAPEVIL- 281
           H    +HRDIKGANILVD  G IKLADFG +K V   +T+    S KG+PYWMAPEV++ 
Sbjct: 334 HGRNTVHRDIKGANILVDPNGEIKLADFGMAKHVTAFSTM---LSFKGSPYWMAPEVVMS 393

Query: 282 QTGHSFSADIWSVGCTVIEMATGKPPWSDQYPEVAALFHIGTTKSHPPIPEQLSIEAQDF 341
           Q G++ + DIWS+GCT++EMAT KPPWS Q+  VAA+F IG +K  P IP+ LS +A++F
Sbjct: 394 QNGYTHAVDIWSLGCTILEMATSKPPWS-QFEGVAAIFKIGNSKDTPEIPDHLSNDAKNF 453

Query: 342 LLKCLQKEPNLRPVASELLKHPFV 365
           +  CLQ+ P +RP AS+LL+HPF+
Sbjct: 454 IRLCLQRNPTVRPTASQLLEHPFL 470