MC08g2203 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g2203
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionRNA pseudouridine synthase 1
LocationMC08: 30860088 .. 30863263 (+)
RNA-Seq ExpressionMC08g2203
SyntenyMC08g2203
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGACCCATCAATTCCGGTGACCGGAAACAGCCCAGCAACAGGACAGAATTATCCAATACCGCTCTCGCCTCCACTACCGGCCATATCCAAGAACTTGGAGCTCGCTAGGGCCATGGCGGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCAGCAACCGATGTTATATTCGAGGACGAGTGGCTCATTGCTGTAAATAAGCCCCAAGGGGTTTACTGTGAAAGCGTTTTGGCCTCTGTTCCTCGTCTTCTCGGTGATTCGGCCATGGCTGGTGAGCAGAGTGGAGGTACTACAACTCTCTGCCTTTGCTTAGCTCGTGAGTTTGCCATTAAGAGTCAATTGAATTGTGTTTGGGTGTTATTTTGGAACTTTTTTTTCTTTTTTTGAGAAGTATTCAAAATTTGGACTGAGCTCGGCAAGCTGAATTCATTTTTACTCTAAACTTTCAACTTCATCGAATTGGATTGCTGCATTGCAATCTGCAACTTGCTCTCATTTTGAATAGTTAAAAAACATTATTACGCTCTTTTCATGAATTACAAAATAGGTATCATGATATGAAAATTGAGACGTGATACCAAATCTTTGAAGCTTCAATCAGGGCTGAATTTTTGTTCAACCTGTTGAGATTTAGGAGCTTTGTTCGACCGTAGATCCTGTGCATTGATTTGCTAAGGCATAACTTTAGTGCATTGAAATGTTATAGCTTGATTTTCATTGAAGTTAGTGGGTGAATGTAATGGAAATATAATGGAAGTCGAACTTATGGTTGGAAAGGAATCTTCGCTCTTTTGAGGATAAGGCTAAGTCCTTTGAGTCATTTTGTGATAGTGTCCATATTAACGCACGCCTCATAGTGGTGTCATACAAATAGGAAGCTCTTTTGCAATTACAACCTTTCTATGATTTTACAGGATTGGAGAGTCATTCTCTCTTAGCTTTTCCTTTTTTGGAGAGGGACTTCTTGTCCCTTGTCCTTAGGCTGTTCTTTGGTTCTTCTGTTTATAATATGTCTCAGTTTCTTATTAAAAAAAAATGGAAGTTATAACAGTCATTTAAGTTTAGGGTTCAATTTGACGAAATTGAAAGTTTGAAATGAATAATTACTCGCTCTGCCAATTTAAAGGGTAAAAATTTGAATTTTTCCAAACTTTTTTAACCATAGAAAAGGAATGCTTTATGGAATCTGTTTTCATTTTTTGAATCTATTTAAAAATGAAATCTCATATGTTCTCATCCCCTTTTTGTTTTTTCACGAAACACCTTGCCCAATGGTTTTGTCAACAAGAATCCATCTTTAGTGTTTTTTAAAACGAACAATATTTTCTACTCAACTTTCAAAGAAGTATTTAGGTAATCTCATAAAATTAATGGTACTGATGTTTTTAGGGGTGCTTAGTGGTATAGTATAGATAGCATGATAGGTGGGGATGCCTTGGGAATTTGACATGAATACATTTACTTAAGTGGTTTCTACTGCTTTCCCTATATTGTGGAAACATTTTGAATGTTTTTTTTTTCATAAGCCAGTTGATGTAAAGGGTTTAGCTGTATTCTGGTTGTTGAACAGAGAGGTGCTTGAGTGTGTCTGAAGTTTAAAAAATTTGTCTAAAATGATGTCATGTATTTACATCTTTTGATTGTTCTATCATTACCAATTTCTCAGATTATTCTTACCTCAGATTCTCCAAATGCAGGGATCCAAATCAGCTTGCCCGAGCTTCATCTTGCTAACCGCCTTGATCGTGACACAAGTGGGGTGATGGTTATAACTAAGTCGCATAAAGTTGCTTCTAAATTGGTGAAGGCATTTACTGACCACAATGTTTCAAAATCATATATTGCCTTCTGTGTTGGCTCTGCTCCGAAATGGAAAAAAATCAATGTCAAATCTGGTCATGGAAGGTCAAAGTTTGGGGTCTGGCGTGTCTATGCTGCAGCAGATGTGGGTCGCTCCTTACCTGGCGGTTCGGTGGTCAGAGACATGGAAACCTATTTTGAAGTACTGTCTGTAAATGGACAAAGCACCATGGACGAACTTCCAGAAGTAAGGAAAGATGAAGAAGAAACTATTGTAGTCCAATCGAAATCTTTGATAGATATTGATGCCCATAAGGACGAGATCTTGATTAGAGCACGACCTCGCAGTGGAAGAACCCATCAAATCCGCCTGCACTGCCAATATCTTGGAATTCCTATTAGAGGAGATGTTAAATACGAGGGTGTCATTGAGTGGAATGGAAATATTTATGACAGCCATGAGCTTCATGCTGAGAGCTTGTTCTTTGTGCACCCTGTTACTGGTATTCCTCTCAAACTGCAGGCTCCTCTACCATCATGGGCAAGTCAAGCATTGCTGTCCCAACAACAAGAAGTGAGCTCTCCATCAACCTTGAAGCATGTGTCTTAACAAGATCATTGGCACAGCTAAATGAGAACCAAAGAATTTTCTTATTAAATGAAGAGCCAGCGAATGCCGCTATAGCAAAATGGGCAATGTTCAATCAACTTCCAGCAAGTGCCACTATGAACGGTGTTCTCACCGTTGGCTCCTTGTCTGCAGTTTCTCATCTACACTTGATATTTCTGCTCCTTATTGAAGCAGAGATTTGAACGAGCTTTTGTGCGCACAGAAGTTTCTACGAAAATATCAGTAGAGTCGTAAACCAACCAACTTGCAGAATTAATCCTGAATATTTGAGAGGATGGATGCTCACAACAGGTTCTAGAAGGGAAAGATGAAGTATGCATGTATAAGGCTCATAAAGTTGTGCAAAGCCCTTCGGGAATGCATCTTCTGCAGGCATTTTATTGATTAAATTTGTGTTGAAGCAGAAAATTCTATGGTTGGGATGTTGTTGTTTTATATTGAGACTCATTGTTATTTCTCTCCCTCTCCGTTCAGTTGAGCAACTTACTGATCAAAATATGAAGGAATAATAAAGCTGTTCATGCAGCAATAAATTATGAATAGAAGAATAAGTAACTATAGAATTTTTATAGAATTTTTTAAGATAAATCTTCCTCTTTTGACATTTTTTGTAATACTCTTCAATTACACTCTTCTTCAATTACACTCTTCTTGGTGGTGTTATAGGCATAGAAAATCCTTTTGTAATTATAGCCTCTAAATGATTATGCAAGATTGGAGAGCTATTCTTCGGTGGGGGGCT

mRNA sequence

ATGGCGGACCCATCAATTCCGGTGACCGGAAACAGCCCAGCAACAGGACAGAATTATCCAATACCGCTCTCGCCTCCACTACCGGCCATATCCAAGAACTTGGAGCTCGCTAGGGCCATGGCGGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCAGCAACCGATGTTATATTCGAGGACGAGTGGCTCATTGCTGTAAATAAGCCCCAAGGGGTTTACTGTGAAAGCGTTTTGGCCTCTGTTCCTCGTCTTCTCGGTGATTCGGCCATGGCTGGGATCCAAATCAGCTTGCCCGAGCTTCATCTTGCTAACCGCCTTGATCGTGACACAAGTGGGGTGATGGTTATAACTAAGTCGCATAAAGTTGCTTCTAAATTGGTGAAGGCATTTACTGACCACAATGTTTCAAAATCATATATTGCCTTCTGTGTTGGCTCTGCTCCGAAATGGAAAAAAATCAATGTCAAATCTGGTCATGGAAGGTCAAAGTTTGGGGTCTGGCGTGTCTATGCTGCAGCAGATGTGGGTCGCTCCTTACCTGGCGGTTCGGTGGTCAGAGACATGGAAACCTATTTTGAAGTACTGTCTGTAAATGGACAAAGCACCATGGACGAACTTCCAGAAGTAAGGAAAGATGAAGAAGAAACTATTGTAGTCCAATCGAAATCTTTGATAGATATTGATGCCCATAAGGACGAGATCTTGATTAGAGCACGACCTCGCAGTGGAAGAACCCATCAAATCCGCCTGCACTGCCAATATCTTGGAATTCCTATTAGAGGAGATGTTAAATACGAGGGTGTCATTGAGTGGAATGGAAATATTTATGACAGCCATGAGCTTCATGCTGAGAGCTTGTTCTTTGTGCACCCTGTTACTGGTATTCCTCTCAAACTGCAGGCTCCTCTACCATCATGGGCAAGTCAAGCATTGCTGTCCCAACAACAAGAAGTGAGCTCTCCATCAACCTTGAAGCATGTGTCTTAACAAGATCATTGGCACAGCTAAATGAGAACCAAAGAATTTTCTTATTAAATGAAGAGCCAGCGAATGCCGCTATAGCAAAATGGGCAATGTTCAATCAACTTCCAGCAAGTGCCACTATGAACGGTGTTCTCACCGTTGGCTCCTTGTCTGCAGTTTCTCATCTACACTTGATATTTCTGCTCCTTATTGAAGCAGAGATTTGAACGAGCTTTTGTGCGCACAGAAGTTTCTACGAAAATATCAGTAGAGTCGTAAACCAACCAACTTGCAGAATTAATCCTGAATATTTGAGAGGATGGATGCTCACAACAGGTTCTAGAAGGGAAAGATGAAGTATGCATGTATAAGGCTCATAAAGTTGTGCAAAGCCCTTCGGGAATGCATCTTCTGCAGGCATTTTATTGATTAAATTTGTGTTGAAGCAGAAAATTCTATGGTTGGGATGTTGTTGTTTTATATTGAGACTCATTGTTATTTCTCTCCCTCTCCGTTCAGTTGAGCAACTTACTGATCAAAATATGAAGGAATAATAAAGCTGTTCATGCAGCAATAAATTATGAATAGAAGAATAAGTAACTATAGAATTTTTATAGAATTTTTTAAGATAAATCTTCCTCTTTTGACATTTTTTGTAATACTCTTCAATTACACTCTTCTTCAATTACACTCTTCTTGGTGGTGTTATAGGCATAGAAAATCCTTTTGTAATTATAGCCTCTAAATGATTATGCAAGATTGGAGAGCTATTCTTCGGTGGGGGGCT

Coding sequence (CDS)

ATGGCGGACCCATCAATTCCGGTGACCGGAAACAGCCCAGCAACAGGACAGAATTATCCAATACCGCTCTCGCCTCCACTACCGGCCATATCCAAGAACTTGGAGCTCGCTAGGGCCATGGCGGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCAGCAACCGATGTTATATTCGAGGACGAGTGGCTCATTGCTGTAAATAAGCCCCAAGGGGTTTACTGTGAAAGCGTTTTGGCCTCTGTTCCTCGTCTTCTCGGTGATTCGGCCATGGCTGGGATCCAAATCAGCTTGCCCGAGCTTCATCTTGCTAACCGCCTTGATCGTGACACAAGTGGGGTGATGGTTATAACTAAGTCGCATAAAGTTGCTTCTAAATTGGTGAAGGCATTTACTGACCACAATGTTTCAAAATCATATATTGCCTTCTGTGTTGGCTCTGCTCCGAAATGGAAAAAAATCAATGTCAAATCTGGTCATGGAAGGTCAAAGTTTGGGGTCTGGCGTGTCTATGCTGCAGCAGATGTGGGTCGCTCCTTACCTGGCGGTTCGGTGGTCAGAGACATGGAAACCTATTTTGAAGTACTGTCTGTAAATGGACAAAGCACCATGGACGAACTTCCAGAAGTAAGGAAAGATGAAGAAGAAACTATTGTAGTCCAATCGAAATCTTTGATAGATATTGATGCCCATAAGGACGAGATCTTGATTAGAGCACGACCTCGCAGTGGAAGAACCCATCAAATCCGCCTGCACTGCCAATATCTTGGAATTCCTATTAGAGGAGATGTTAAATACGAGGGTGTCATTGAGTGGAATGGAAATATTTATGACAGCCATGAGCTTCATGCTGAGAGCTTGTTCTTTGTGCACCCTGTTACTGGTATTCCTCTCAAACTGCAGGCTCCTCTACCATCATGGGCAAGTCAAGCATTGCTGTCCCAACAACAAGAAGTGAGCTCTCCATCAACCTTGAAGCATGTGTCTTAA

Protein sequence

MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFEDEWLIAVNKPQGVYCESVLASVPRLLGDSAMAGIQISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGIPLKLQAPLPSWASQALLSQQQEVSSPSTLKHVS
Homology
[Close]
BLAST of MC08g2203 vs. ExPASy Swiss-Prot
Match: Q2QNM3 (RNA pseudouridine synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0560500 PE=2 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 9.4e-101
Identity = 186/302 (61.59%), Postives = 229/302 (75.83%), Query Frame = 0 

Query: 12  SPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFEDEWLIAVNKPQG 71
           S ATG+ YP+P+SPP PA SK++EL RAM AS++S+  A S+  V+FEDEWL  V+KP G
Sbjct: 45  SAATGE-YPVPVSPPYPAASKDVELRRAMTASARSA--AYSSAPVVFEDEWLAVVDKPAG 104

Query: 72  VYCESVLASVPRLLGDSAMAGIQISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFT 131
           VYC+++L+++P     +A  G + + P LHLANRLDRDTSG+MVITK +KVA KLVKAFT
Sbjct: 105 VYCDALLSALP---CSAATLGDEATKPNLHLANRLDRDTSGLMVITKCNKVAGKLVKAFT 164

Query: 132 DHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETY 191
           +H V K+Y+A C+G  P W+KI + SGHGRSK G WRVYA +DVGRSLPGGSVVRDM T 
Sbjct: 165 EHKVKKTYLALCIGYPPAWEKIKICSGHGRSKHGAWRVYAMSDVGRSLPGGSVVRDMSTR 224

Query: 192 FEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDE----ILIRARPRSGRTHQ 251
           FEVL +NG+    E      DE E+I VQ K+  D+ +  DE    IL+RA P+SGRTHQ
Sbjct: 225 FEVLGINGKGQFREPSNFEVDETESITVQEKA-ADLTSDGDEKNSIILVRAYPQSGRTHQ 284

Query: 252 IRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGIPLKLQAPLPSWA 310
           IRLHCQYLG PIRGDVKY GVIEWNG  YD H LHAESL FVHPVTG+P+  ++PLPSWA
Sbjct: 285 IRLHCQYLGFPIRGDVKYSGVIEWNGVDYDGHALHAESLSFVHPVTGLPVTFRSPLPSWA 339

[Close]
BLAST of MC08g2203 vs. ExPASy Swiss-Prot
Match: Q7XA65 (RNA pseudouridine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g56345 PE=2 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 5.7e-98
Identity = 181/293 (61.77%), Postives = 222/293 (75.77%), Query Frame = 0 

Query: 18  NYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFEDEWLIAVNKPQGVYCESV 77
           NYP P+S P P ISK++EL RAM ASSKSSLF L+  D+++EDE+L+AVNKP+GVYCE+V
Sbjct: 39  NYPKPISAPPPPISKDIELRRAMEASSKSSLFNLTRDDILYEDEYLMAVNKPKGVYCEAV 98

Query: 78  LASVPRLLGDSAMAGIQISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHNVSK 137
           L S P+++ DS+         E HLANRLDRDTSGVM+ITKSHKVA+KLVKAFT+H + K
Sbjct: 99  LRSAPQIVLDSS--------SEYHLANRLDRDTSGVMIITKSHKVAAKLVKAFTEHKIRK 158

Query: 138 SYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSV 197
           SYIA C+GS+P W+++ V SGHGRSK G WRVYAA DVGR LPGGS VRDMET FEV+SV
Sbjct: 159 SYIALCIGSSPNWRRVTVSSGHGRSKHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSV 218

Query: 198 NGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDE--ILIRARPRSGRTHQIRLHCQYL 257
           N  S  +E  E+ +D    IV + +  +      D+  +++RA PRSGRTHQIRLHCQYL
Sbjct: 219 N--SVKNESCEL-EDVNHVIVAEGERELSCGGDDDDVVVVVRAFPRSGRTHQIRLHCQYL 278

Query: 258 GIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGIPLKLQAPLPSWAS 309
           GIPIRGDVKY GV EWNG  ++ HELHAE L   HPVTG  + ++APLP WA+
Sbjct: 279 GIPIRGDVKYHGVYEWNGRTFEGHELHAECLSLDHPVTGDSIVIRAPLPYWAA 320

[Close]
BLAST of MC08g2203 vs. ExPASy Swiss-Prot
Match: Q68XB2 (Ribosomal large subunit pseudouridine synthase C OS=Rickettsia typhi (strain ATCC VR-144 / Wilmington) OX=257363 GN=rluC PE=3 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 1.0e-17
Identity = 79/258 (30.62%), Postives = 125/258 (48.45%), Query Frame = 0 

Query: 56  VIFEDEWLIAVNKPQGVYCESVLASVPRLLGDSAMAGIQISLPELHLANRLDRDTSGVMV 115
           +I+ED+ LIA+NKP  +  +    S   L  DSA+  +     +  L +RLD++TSG+++
Sbjct: 91  LIYEDDHLIAINKPASLATQG--GSKINLSIDSALKYLNYQGADFKLVHRLDKETSGLLL 150

Query: 116 ITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADV 175
           I K++  + KL  AF +  V K Y A   G   K   I VKS  G+SK    R++   D+
Sbjct: 151 IAKNYLSSVKLHDAFKEKLVIKKYFAITYGRPVKNVGI-VKSNIGKSK---GRMFKITDI 210

Query: 176 GRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEIL 235
                 G +     TY+++L                          KSL     + +  L
Sbjct: 211 DSD--NGKLA---ITYYKLL--------------------------KSL-----NNNLFL 270

Query: 236 IRARPRSGRTHQIRLHCQYLGIPIRGDVKY--EGVIEWNGNIYDSHELHAESLFFVHPVT 295
           I   P +GR HQ+RLH Q LG PI GD KY  + V+ ++  ++    LHA +++    + 
Sbjct: 271 IEFMPVTGRMHQLRLHAQLLGCPILGDDKYGNKEVMPYSKYMF----LHANNIYLSESIV 302

Query: 296 GIPLKLQAPLPSWASQAL 312
           G  +KL+A LP + ++ L
Sbjct: 331 GKEIKLEAKLPFYFTRRL 302

[Close]
BLAST of MC08g2203 vs. ExPASy Swiss-Prot
Match: Q8PHN2 (Ribosomal large subunit pseudouridine synthase D OS=Xanthomonas axonopodis pv. citri (strain 306) OX=190486 GN=rluD PE=3 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.4e-16
Identity = 72/266 (27.07%), Postives = 116/266 (43.61%), Query Frame = 0 

Query: 56  VIFEDEWLIAVNKPQGVYCESVLASVPR------LLGDSAMAGIQISLPELHLANRLDRD 115
           V++EDE ++ ++KP G+       +  R      L  D A+A    S+P   + +RLD+D
Sbjct: 92  VLYEDEQVLVIDKPAGLVVHPGAGNPDRTLVNALLFRDPALA----SVPRAGVVHRLDKD 151

Query: 116 TSGVMVITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRV 175
           TSGVMV+ ++ +  + LV+  +   V + Y+A  VG+                       
Sbjct: 152 TSGVMVVARTVQAQTALVEQLSAREVHRQYLAVVVGAL---------------------- 211

Query: 176 YAAADVGRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDA 235
                    + GG+    ++ +            D L    +D+    V   +      A
Sbjct: 212 ---------VSGGTANAPIDRH----------PRDRLKMAVRDDGRDAVTHYRLRERFRA 271

Query: 236 HKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDS--HE------- 295
           H     +  R  +GRTHQIR+H  +L  PI GD  Y G ++      D   HE       
Sbjct: 272 H---TALECRLETGRTHQIRVHMAHLKSPIVGDPLYGGALKLPKGATDELVHELRTFKRQ 309

Query: 296 -LHAESLFFVHPVTGIPLKLQAPLPS 306
            LHAE+L F+HPV+G P++  AP+P+
Sbjct: 332 ALHAETLEFLHPVSGEPVRASAPVPA 309

[Close]
BLAST of MC08g2203 vs. ExPASy Swiss-Prot
Match: Q9ZDR7 (Ribosomal large subunit pseudouridine synthase C OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=rluC PE=3 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.9e-16
Identity = 70/259 (27.03%), Postives = 120/259 (46.33%), Query Frame = 0 

Query: 56  VIFEDEWLIAVNKPQGVYCESVLASVPRLLGDSAMAGIQISLPELHLANRLDRDTSGVMV 115
           +I+ED+ LIA+NKP  +  +    S   L  DSA+  +     +  L +RLD++TSG+++
Sbjct: 91  LIYEDDHLIAINKPASLATQG--GSKINLSIDSALKYLNYQGADFKLVHRLDKETSGLLL 150

Query: 116 ITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADV 175
           I K++  + KL  AF +  V K Y A   G  P      VKS  G+SK            
Sbjct: 151 IAKNYLSSVKLHNAFKEKLVIKKYFAITYG-RPVKNVGTVKSNIGKSK------------ 210

Query: 176 GRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEIL 235
                                      + ++  +  D+ +  +   K L  +  + +  L
Sbjct: 211 -------------------------RRLSKIANIDSDDGKLAITYYKLLKSL--NNNLFL 270

Query: 236 IRARPRSGRTHQIRLHCQYLGIPIRGDVKY--EGVIEWNGNIYDSHELHAESLFFVHPVT 295
           I   P +GR HQ+RLH Q LG PI GD KY  + ++ ++  ++    LHA +++    + 
Sbjct: 271 IEFMPVTGRMHQLRLHAQLLGCPILGDDKYGNKEIMPYSKYMF----LHANNIYLSETIV 303

Query: 296 GIPLKLQAPLPSWASQALL 313
           G  +KL+A LP + ++ ++
Sbjct: 331 GKEIKLEAKLPFYFTRRII 303

[Close]
BLAST of MC08g2203 vs. NCBI nr
Match: XP_022156345.1 (RNA pseudouridine synthase 1 isoform X2 [Momordica charantia])

HSP 1 Score: 641 bits (1654), Expect = 1.12e-231
Identity = 328/333 (98.50%), Postives = 328/333 (98.50%), Query Frame = 0 

Query: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60
           MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED
Sbjct: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60

Query: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAG-----IQISLPELHLANRLDRDTSGVMV 120
           EWLIAVNKPQGVYCESVLASVPRLLGDSAMAG     IQISLPELHLANRLDRDTSGVMV
Sbjct: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGEQSGGIQISLPELHLANRLDRDTSGVMV 120

Query: 121 ITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADV 180
           ITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADV
Sbjct: 121 ITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADV 180

Query: 181 GRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEIL 240
           GRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEIL
Sbjct: 181 GRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEIL 240

Query: 241 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGI 300
           IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGI
Sbjct: 241 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGI 300

Query: 301 PLKLQAPLPSWASQALLSQQQEVSSPSTLKHVS 328
           PLKLQAPLPSWASQALLSQQQEVSSPSTLKHVS
Sbjct: 301 PLKLQAPLPSWASQALLSQQQEVSSPSTLKHVS 333

[Close]
BLAST of MC08g2203 vs. NCBI nr
Match: XP_022156344.1 (RNA pseudouridine synthase 1 isoform X1 [Momordica charantia])

HSP 1 Score: 637 bits (1644), Expect = 5.46e-230
Identity = 328/343 (95.63%), Postives = 328/343 (95.63%), Query Frame = 0 

Query: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60
           MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED
Sbjct: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60

Query: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAG---------------IQISLPELHLANR 120
           EWLIAVNKPQGVYCESVLASVPRLLGDSAMAG               IQISLPELHLANR
Sbjct: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGEQSGGTTTLCLCLARIQISLPELHLANR 120

Query: 121 LDRDTSGVMVITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFG 180
           LDRDTSGVMVITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFG
Sbjct: 121 LDRDTSGVMVITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFG 180

Query: 181 VWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLI 240
           VWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLI
Sbjct: 181 VWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLI 240

Query: 241 DIDAHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAES 300
           DIDAHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAES
Sbjct: 241 DIDAHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAES 300

Query: 301 LFFVHPVTGIPLKLQAPLPSWASQALLSQQQEVSSPSTLKHVS 328
           LFFVHPVTGIPLKLQAPLPSWASQALLSQQQEVSSPSTLKHVS
Sbjct: 301 LFFVHPVTGIPLKLQAPLPSWASQALLSQQQEVSSPSTLKHVS 343

[Close]
BLAST of MC08g2203 vs. NCBI nr
Match: XP_008453651.1 (PREDICTED: RNA pseudouridine synthase 1 [Cucumis melo] >KAA0048062.1 RNA pseudouridine synthase 1 [Cucumis melo var. makuwa] >TYJ96474.1 RNA pseudouridine synthase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 568 bits (1464), Expect = 8.41e-203
Identity = 279/325 (85.85%), Postives = 305/325 (93.85%), Query Frame = 0 

Query: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60
           M D S PVTGN P+TG+NYP+PLSPPLPAISKNLELARAMAASSKSSLFALSA DVI+ED
Sbjct: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60

Query: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGIQISLPELHLANRLDRDTSGVMVITKSH 120
           EWLIAVNKPQG+YCE+VLA+VPRLL DSA AGI+ +LPELHLANRLDRDTSGVMVITKSH
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH 120

Query: 121 KVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLP 180
           KVASKLVKAFTDH VSKSYIAFCVG++PKWKKI+VKSGHGRSKFGVWRVYAAADVGRSLP
Sbjct: 121 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 180

Query: 181 GGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEILIRARP 240
           GGS+VRDMETYFEVLSVNG++TM+EL + RK+EEETIVV +KSL+DID+ KDE+LIRARP
Sbjct: 181 GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP 240

Query: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGIPLKLQ 300
           RSGRTHQIRLHCQYLGIPIRGDVKYEGV EWNG  YDSHELHAESL+FVHP+TGIPLKLQ
Sbjct: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ 300

Query: 301 APLPSWASQALLSQQQEVSSPSTLK 325
           APLPSWASQAL  QQQEV+SP + K
Sbjct: 301 APLPSWASQALQPQQQEVNSPQSFK 325

[Close]
BLAST of MC08g2203 vs. NCBI nr
Match: XP_004144814.3 (RNA pseudouridine synthase 1 [Cucumis sativus] >KGN60992.1 hypothetical protein Csa_021313 [Cucumis sativus])

HSP 1 Score: 568 bits (1465), Expect = 2.39e-202
Identity = 281/325 (86.46%), Postives = 303/325 (93.23%), Query Frame = 0 

Query: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60
           M D S PVTGN P+T +NYP+PLSPPLPAISKNLELARAM ASSKSSL+ALSA DVI+ED
Sbjct: 39  MTDSSEPVTGNCPSTAENYPVPLSPPLPAISKNLELARAMVASSKSSLYALSANDVIYED 98

Query: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGIQISLPELHLANRLDRDTSGVMVITKSH 120
           EWLIAVNKPQG+YCE+VLA+VPRLLGDSA AGI+ SLPELHLANRLDRDTSGVMVITKSH
Sbjct: 99  EWLIAVNKPQGIYCENVLAAVPRLLGDSANAGIKTSLPELHLANRLDRDTSGVMVITKSH 158

Query: 121 KVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLP 180
           KVASKLVKAFTDH VSKSYIAFCVG++PKWKKINVKSGHGRSKFGVWRVYAAADVGRSLP
Sbjct: 159 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLP 218

Query: 181 GGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEILIRARP 240
           GGSVVRDMETYFEVLSVNG++TM+EL + R+ EEETIVV +KSL+DID+HKDEILIRARP
Sbjct: 219 GGSVVRDMETYFEVLSVNGKNTMEELQKFRRGEEETIVVHTKSLVDIDSHKDEILIRARP 278

Query: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGIPLKLQ 300
           RSGRTHQIRLHCQYLGIPIRGDVKYEGV EWN  IYDSHELHAESL+FVHPVTGIPLKLQ
Sbjct: 279 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNEKIYDSHELHAESLYFVHPVTGIPLKLQ 338

Query: 301 APLPSWASQALLSQQQEVSSPSTLK 325
           APLPSWASQAL  QQ EV+SP + K
Sbjct: 339 APLPSWASQALQPQQHEVNSPQSFK 363

[Close]
BLAST of MC08g2203 vs. NCBI nr
Match: XP_038889468.1 (RNA pseudouridine synthase 1 [Benincasa hispida])

HSP 1 Score: 565 bits (1455), Expect = 5.53e-201
Identity = 281/321 (87.54%), Postives = 299/321 (93.15%), Query Frame = 0 

Query: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60
           MAD S PVTGN P+T +NYP+PLSPPLPAISKNLELARAMAASSKSSLFALSA DVI+ED
Sbjct: 37  MADSSEPVTGNCPSTRENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 96

Query: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGIQISLPELHLANRLDRDTSGVMVITKSH 120
           EWLIAVNKPQG+YCESVLA+VPRLL DSAMAGI+ SLPELHLANRLDRDTSGVMVITKSH
Sbjct: 97  EWLIAVNKPQGIYCESVLAAVPRLLSDSAMAGIKTSLPELHLANRLDRDTSGVMVITKSH 156

Query: 121 KVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLP 180
           KVASKLVKAFTDH VSKSYIAFCVGSAPKWKKIN+KSGHGRSKFGVWRVYAAADVGRSLP
Sbjct: 157 KVASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLP 216

Query: 181 GGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEILIRARP 240
           GGSVVRDMETYFEVLS+NGQ+ M+E  +  KDEE T+VVQ+KSL+DID  KDEILIRARP
Sbjct: 217 GGSVVRDMETYFEVLSINGQNAMEEFQKFGKDEE-TVVVQTKSLVDIDGQKDEILIRARP 276

Query: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGIPLKLQ 300
           RSGRTHQIRLHCQYLGIPIRGDVKYEGV EWNG IYD HELHAESL+FVHP+TGIPLKLQ
Sbjct: 277 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKIYDCHELHAESLYFVHPITGIPLKLQ 336

Query: 301 APLPSWASQALLSQQQEVSSP 321
           APLPSWASQA   QQQEV+SP
Sbjct: 337 APLPSWASQAWQPQQQEVNSP 356

[Close]
BLAST of MC08g2203 vs. ExPASy TrEMBL
Match: A0A6J1DQ12 (RNA pseudouridine synthase 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023261 PE=4 SV=1)

HSP 1 Score: 641 bits (1654), Expect = 5.45e-232
Identity = 328/333 (98.50%), Postives = 328/333 (98.50%), Query Frame = 0 

Query: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60
           MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED
Sbjct: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60

Query: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAG-----IQISLPELHLANRLDRDTSGVMV 120
           EWLIAVNKPQGVYCESVLASVPRLLGDSAMAG     IQISLPELHLANRLDRDTSGVMV
Sbjct: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGEQSGGIQISLPELHLANRLDRDTSGVMV 120

Query: 121 ITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADV 180
           ITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADV
Sbjct: 121 ITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADV 180

Query: 181 GRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEIL 240
           GRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEIL
Sbjct: 181 GRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEIL 240

Query: 241 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGI 300
           IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGI
Sbjct: 241 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGI 300

Query: 301 PLKLQAPLPSWASQALLSQQQEVSSPSTLKHVS 328
           PLKLQAPLPSWASQALLSQQQEVSSPSTLKHVS
Sbjct: 301 PLKLQAPLPSWASQALLSQQQEVSSPSTLKHVS 333

[Close]
BLAST of MC08g2203 vs. ExPASy TrEMBL
Match: A0A6J1DT56 (RNA pseudouridine synthase 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023261 PE=4 SV=1)

HSP 1 Score: 637 bits (1644), Expect = 2.64e-230
Identity = 328/343 (95.63%), Postives = 328/343 (95.63%), Query Frame = 0 

Query: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60
           MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED
Sbjct: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60

Query: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAG---------------IQISLPELHLANR 120
           EWLIAVNKPQGVYCESVLASVPRLLGDSAMAG               IQISLPELHLANR
Sbjct: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGEQSGGTTTLCLCLARIQISLPELHLANR 120

Query: 121 LDRDTSGVMVITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFG 180
           LDRDTSGVMVITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFG
Sbjct: 121 LDRDTSGVMVITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFG 180

Query: 181 VWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLI 240
           VWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLI
Sbjct: 181 VWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLI 240

Query: 241 DIDAHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAES 300
           DIDAHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAES
Sbjct: 241 DIDAHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAES 300

Query: 301 LFFVHPVTGIPLKLQAPLPSWASQALLSQQQEVSSPSTLKHVS 328
           LFFVHPVTGIPLKLQAPLPSWASQALLSQQQEVSSPSTLKHVS
Sbjct: 301 LFFVHPVTGIPLKLQAPLPSWASQALLSQQQEVSSPSTLKHVS 343

[Close]
BLAST of MC08g2203 vs. ExPASy TrEMBL
Match: A0A5A7TX25 (RNA pseudouridine synthase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001190 PE=4 SV=1)

HSP 1 Score: 568 bits (1464), Expect = 4.07e-203
Identity = 279/325 (85.85%), Postives = 305/325 (93.85%), Query Frame = 0 

Query: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60
           M D S PVTGN P+TG+NYP+PLSPPLPAISKNLELARAMAASSKSSLFALSA DVI+ED
Sbjct: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60

Query: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGIQISLPELHLANRLDRDTSGVMVITKSH 120
           EWLIAVNKPQG+YCE+VLA+VPRLL DSA AGI+ +LPELHLANRLDRDTSGVMVITKSH
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH 120

Query: 121 KVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLP 180
           KVASKLVKAFTDH VSKSYIAFCVG++PKWKKI+VKSGHGRSKFGVWRVYAAADVGRSLP
Sbjct: 121 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 180

Query: 181 GGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEILIRARP 240
           GGS+VRDMETYFEVLSVNG++TM+EL + RK+EEETIVV +KSL+DID+ KDE+LIRARP
Sbjct: 181 GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP 240

Query: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGIPLKLQ 300
           RSGRTHQIRLHCQYLGIPIRGDVKYEGV EWNG  YDSHELHAESL+FVHP+TGIPLKLQ
Sbjct: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ 300

Query: 301 APLPSWASQALLSQQQEVSSPSTLK 325
           APLPSWASQAL  QQQEV+SP + K
Sbjct: 301 APLPSWASQALQPQQQEVNSPQSFK 325

[Close]
BLAST of MC08g2203 vs. ExPASy TrEMBL
Match: A0A1S3BW87 (RNA pseudouridine synthase 1 OS=Cucumis melo OX=3656 GN=LOC103494301 PE=4 SV=1)

HSP 1 Score: 568 bits (1464), Expect = 4.07e-203
Identity = 279/325 (85.85%), Postives = 305/325 (93.85%), Query Frame = 0 

Query: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60
           M D S PVTGN P+TG+NYP+PLSPPLPAISKNLELARAMAASSKSSLFALSA DVI+ED
Sbjct: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60

Query: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGIQISLPELHLANRLDRDTSGVMVITKSH 120
           EWLIAVNKPQG+YCE+VLA+VPRLL DSA AGI+ +LPELHLANRLDRDTSGVMVITKSH
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH 120

Query: 121 KVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLP 180
           KVASKLVKAFTDH VSKSYIAFCVG++PKWKKI+VKSGHGRSKFGVWRVYAAADVGRSLP
Sbjct: 121 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 180

Query: 181 GGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEILIRARP 240
           GGS+VRDMETYFEVLSVNG++TM+EL + RK+EEETIVV +KSL+DID+ KDE+LIRARP
Sbjct: 181 GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP 240

Query: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGIPLKLQ 300
           RSGRTHQIRLHCQYLGIPIRGDVKYEGV EWNG  YDSHELHAESL+FVHP+TGIPLKLQ
Sbjct: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ 300

Query: 301 APLPSWASQALLSQQQEVSSPSTLK 325
           APLPSWASQAL  QQQEV+SP + K
Sbjct: 301 APLPSWASQALQPQQQEVNSPQSFK 325

[Close]
BLAST of MC08g2203 vs. ExPASy TrEMBL
Match: A0A0A0LJY6 (PseudoU_synth_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G033960 PE=4 SV=1)

HSP 1 Score: 568 bits (1465), Expect = 1.16e-202
Identity = 281/325 (86.46%), Postives = 303/325 (93.23%), Query Frame = 0 

Query: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60
           M D S PVTGN P+T +NYP+PLSPPLPAISKNLELARAM ASSKSSL+ALSA DVI+ED
Sbjct: 39  MTDSSEPVTGNCPSTAENYPVPLSPPLPAISKNLELARAMVASSKSSLYALSANDVIYED 98

Query: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGIQISLPELHLANRLDRDTSGVMVITKSH 120
           EWLIAVNKPQG+YCE+VLA+VPRLLGDSA AGI+ SLPELHLANRLDRDTSGVMVITKSH
Sbjct: 99  EWLIAVNKPQGIYCENVLAAVPRLLGDSANAGIKTSLPELHLANRLDRDTSGVMVITKSH 158

Query: 121 KVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLP 180
           KVASKLVKAFTDH VSKSYIAFCVG++PKWKKINVKSGHGRSKFGVWRVYAAADVGRSLP
Sbjct: 159 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLP 218

Query: 181 GGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEILIRARP 240
           GGSVVRDMETYFEVLSVNG++TM+EL + R+ EEETIVV +KSL+DID+HKDEILIRARP
Sbjct: 219 GGSVVRDMETYFEVLSVNGKNTMEELQKFRRGEEETIVVHTKSLVDIDSHKDEILIRARP 278

Query: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGIPLKLQ 300
           RSGRTHQIRLHCQYLGIPIRGDVKYEGV EWN  IYDSHELHAESL+FVHPVTGIPLKLQ
Sbjct: 279 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNEKIYDSHELHAESLYFVHPVTGIPLKLQ 338

Query: 301 APLPSWASQALLSQQQEVSSPSTLK 325
           APLPSWASQAL  QQ EV+SP + K
Sbjct: 339 APLPSWASQALQPQQHEVNSPQSFK 363

[Close]
BLAST of MC08g2203 vs. TAIR 10
Match: AT1G56345.1 (Pseudouridine synthase family protein )

HSP 1 Score: 359.0 bits (920), Expect = 4.1e-99
Identity = 181/293 (61.77%), Postives = 222/293 (75.77%), Query Frame = 0 

Query: 18  NYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFEDEWLIAVNKPQGVYCESV 77
           NYP P+S P P ISK++EL RAM ASSKSSLF L+  D+++EDE+L+AVNKP+GVYCE+V
Sbjct: 39  NYPKPISAPPPPISKDIELRRAMEASSKSSLFNLTRDDILYEDEYLMAVNKPKGVYCEAV 98

Query: 78  LASVPRLLGDSAMAGIQISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHNVSK 137
           L S P+++ DS+         E HLANRLDRDTSGVM+ITKSHKVA+KLVKAFT+H + K
Sbjct: 99  LRSAPQIVLDSS--------SEYHLANRLDRDTSGVMIITKSHKVAAKLVKAFTEHKIRK 158

Query: 138 SYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSV 197
           SYIA C+GS+P W+++ V SGHGRSK G WRVYAA DVGR LPGGS VRDMET FEV+SV
Sbjct: 159 SYIALCIGSSPNWRRVTVSSGHGRSKHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSV 218

Query: 198 NGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDE--ILIRARPRSGRTHQIRLHCQYL 257
           N  S  +E  E+ +D    IV + +  +      D+  +++RA PRSGRTHQIRLHCQYL
Sbjct: 219 N--SVKNESCEL-EDVNHVIVAEGERELSCGGDDDDVVVVVRAFPRSGRTHQIRLHCQYL 278

Query: 258 GIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGIPLKLQAPLPSWAS 309
           GIPIRGDVKY GV EWNG  ++ HELHAE L   HPVTG  + ++APLP WA+
Sbjct: 279 GIPIRGDVKYHGVYEWNGRTFEGHELHAECLSLDHPVTGDSIVIRAPLPYWAA 320

[Close]
BLAST of MC08g2203 vs. TAIR 10
Match: AT1G76050.2 (Pseudouridine synthase family protein )

HSP 1 Score: 64.7 bits (156), Expect = 1.6e-10
Identity = 59/224 (26.34%), Postives = 91/224 (40.62%), Query Frame = 0 

Query: 102 LANRLDRDTSGVMVITKSHKVASKLVKAFTDHNVSKSYIAFCVG-SAPKWKKINVKSGHG 161
           + +RLD+ T+G++V+ K     + L + F  H + + Y++   G  +P   +I +  G  
Sbjct: 229 IVHRLDKGTTGLLVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRD 288

Query: 162 RSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQ 221
            S     R+  AA     +PGG                          VR         +
Sbjct: 289 SSN----RIRMAA-----IPGG--------------------------VRGGRARHAASR 348

Query: 222 SKSLIDIDAHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEG-------VIEWNG 281
            K +I+  A     L+  R  +GRTHQIR H +Y+G+P+ GD  Y G       +++   
Sbjct: 349 YK-VIETFAGGGSALVEWRLETGRTHQIRAHAKYMGVPLLGDEVYGGTKSMALSLLQKRV 408

Query: 282 NIYDSHE------------LHAESLFFVHPVTGIPLKLQAPLPS 306
           +  D  E            LHA  L F HP TG  +K   P PS
Sbjct: 409 SRSDQEEIIELISRMDRPCLHAIVLGFTHPCTGEIVKFSCPPPS 416

[Close]
BLAST of MC08g2203 vs. TAIR 10
Match: AT3G52260.2 (Pseudouridine synthase family protein )

HSP 1 Score: 55.5 bits (132), Expect = 9.6e-08
Identity = 55/219 (25.11%), Postives = 92/219 (42.01%), Query Frame = 0 

Query: 55  DVIFEDEWLIAVNKPQGVYC--------ESVLASVPRLLGDSAMAGIQISLPELHLANRL 114
           +V++ED+ LIA+NKP G+           +VL  +    G +         P     +RL
Sbjct: 98  EVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRESPHPVPVHRL 157

Query: 115 DRDTSGVMVITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGV 174
            R TSG+++  K+    +KL   F +           VGS      ++ + G GR     
Sbjct: 158 GRGTSGILLCAKTKLAKTKLAAYFAEG-------TSLVGSG----NLDQECGTGRK---- 217

Query: 175 WRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLID 234
                 + + R+L  G V  D     + + V     + +   V   E +        +++
Sbjct: 218 -----LSKIYRALADGIVEEDEVVIKQPIGVVRYPGVAQGLYVASPEGKP-AFSKVFVLE 277

Query: 235 IDAHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKY 266
            D  K+  L++   +SGR HQIR+H  Y+G P+ GD  Y
Sbjct: 278 RDREKNCSLVKVEIQSGRPHQIRIHLAYMGHPLVGDPLY 295

[Close]
BLAST of MC08g2203 vs. TAIR 10
Match: AT3G52260.1 (Pseudouridine synthase family protein )

HSP 1 Score: 50.4 bits (119), Expect = 3.1e-06
Identity = 52/213 (24.41%), Postives = 89/213 (41.78%), Query Frame = 0 

Query: 55  DVIFEDEWLIAVNKPQGVYC--------ESVLASVPRLLGDSAMAGIQISLPELHLANRL 114
           +V++ED+ LIA+NKP G+           +VL  +    G +         P     +RL
Sbjct: 98  EVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRESPHPVPVHRL 157

Query: 115 DRDTSGVMVITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGV 174
            R TSG+++  K+    +KL   F +           VGS      ++ + G GR     
Sbjct: 158 GRGTSGILLCAKTKLAKTKLAAYFAEG-------TSLVGSG----NLDQECGTGRK---- 217

Query: 175 WRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLID 234
                 + + R+L  G V  D     + + V     + +   V   E +        +++
Sbjct: 218 -----LSKIYRALADGIVEEDEVVIKQPIGVVRYPGVAQGLYVASPEGKP-AFSKVFVLE 277

Query: 235 IDAHKDEILIRARPRSGRTHQIRLHCQYLGIPI 260
            D  K+  L++   +SGR HQIR+H  Y+G P+
Sbjct: 278 RDREKNCSLVKVEIQSGRPHQIRIHLAYMGHPL 289

[Close]
BLAST of MC08g2203 vs. TAIR 10
Match: AT3G52260.3 (Pseudouridine synthase family protein )

HSP 1 Score: 50.4 bits (119), Expect = 3.1e-06
Identity = 52/213 (24.41%), Postives = 89/213 (41.78%), Query Frame = 0 

Query: 55  DVIFEDEWLIAVNKPQGVYC--------ESVLASVPRLLGDSAMAGIQISLPELHLANRL 114
           +V++ED+ LIA+NKP G+           +VL  +    G +         P     +RL
Sbjct: 128 EVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRESPHPVPVHRL 187

Query: 115 DRDTSGVMVITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGV 174
            R TSG+++  K+    +KL   F +           VGS      ++ + G GR     
Sbjct: 188 GRGTSGILLCAKTKLAKTKLAAYFAEG-------TSLVGSG----NLDQECGTGRK---- 247

Query: 175 WRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLID 234
                 + + R+L  G V  D     + + V     + +   V   E +        +++
Sbjct: 248 -----LSKIYRALADGIVEEDEVVIKQPIGVVRYPGVAQGLYVASPEGKP-AFSKVFVLE 307

Query: 235 IDAHKDEILIRARPRSGRTHQIRLHCQYLGIPI 260
            D  K+  L++   +SGR HQIR+H  Y+G P+
Sbjct: 308 RDREKNCSLVKVEIQSGRPHQIRIHLAYMGHPL 319

The following BLAST results are available for this feature:
BLAST of MC08g2203 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q2QNM39.4e-10161.59RNA pseudouridine synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g05... [more]
Q7XA655.7e-9861.77RNA pseudouridine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g56345 PE=2 S... [more]
Q68XB21.0e-1730.62Ribosomal large subunit pseudouridine synthase C OS=Rickettsia typhi (strain ATC... [more]
Q8PHN21.4e-1627.07Ribosomal large subunit pseudouridine synthase D OS=Xanthomonas axonopodis pv. c... [more]
Q9ZDR71.9e-1627.03Ribosomal large subunit pseudouridine synthase C OS=Rickettsia prowazekii (strai... [more]
BLAST of MC08g2203 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022156345.11.12e-23198.50RNA pseudouridine synthase 1 isoform X2 [Momordica charantia][more]
XP_022156344.15.46e-23095.63RNA pseudouridine synthase 1 isoform X1 [Momordica charantia][more]
XP_008453651.18.41e-20385.85PREDICTED: RNA pseudouridine synthase 1 [Cucumis melo] >KAA0048062.1 RNA pseudou... [more]
XP_004144814.32.39e-20286.46RNA pseudouridine synthase 1 [Cucumis sativus] >KGN60992.1 hypothetical protein ... [more]
XP_038889468.15.53e-20187.54RNA pseudouridine synthase 1 [Benincasa hispida][more]
BLAST of MC08g2203 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1DQ125.45e-23298.50RNA pseudouridine synthase 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1DT562.64e-23095.63RNA pseudouridine synthase 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A5A7TX254.07e-20385.85RNA pseudouridine synthase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3BW874.07e-20385.85RNA pseudouridine synthase 1 OS=Cucumis melo OX=3656 GN=LOC103494301 PE=4 SV=1[more]
A0A0A0LJY61.16e-20286.46PseudoU_synth_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G03... [more]
BLAST of MC08g2203 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G56345.14.1e-9961.77Pseudouridine synthase family protein [more]
AT1G76050.21.6e-1026.34Pseudouridine synthase family protein [more]
AT3G52260.29.6e-0825.11Pseudouridine synthase family protein [more]
AT3G52260.13.1e-0624.41Pseudouridine synthase family protein [more]
AT3G52260.33.1e-0624.41Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.2350.10Pseudouridine synthasecoord: 51..304
e-value: 6.3E-44
score: 152.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availablePANTHERPTHR21600:SF47RNA PSEUDOURIDINE SYNTHASE 1coord: 30..314
NoneNo IPR availablePANTHERPTHR21600MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASEcoord: 30..314
IPR006145Pseudouridine synthase, RsuA/RluA-likePFAMPF00849PseudoU_synth_2coord: 63..253
e-value: 4.4E-22
score: 78.9
IPR006145Pseudouridine synthase, RsuA/RluA-likeCDDcd02869PseudoU_synth_RluA_likecoord: 63..288
e-value: 5.94152E-38
score: 131.691
IPR006224Pseudouridine synthase, RluC/RluD, conserved sitePROSITEPS01129PSI_RLUcoord: 103..117
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 53..310

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g2203.1MC08g2203.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding