MC08g2174 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g2174
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionAminotran_5 domain-containing protein
LocationMC08: 30647768 .. 30652397 (+)
RNA-Seq ExpressionMC08g2174
SyntenyMC08g2174
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCCACTGTGAAAAAAATCATCTTCAGCCCGATTCTGCCCCCCATCTCAACGGTGACGCCAATACCCACTTGCCCAAGAAGCCCAAACTCGCCCCCACTTCCATTTCTGATTCCGAAATTCAATCTGAGTTCTCCCACCATGACCCTTCTGTTGCCCGAATCAACAACGGCAGCTTCGGCTGCTGCCCTTCTTCTGTCATCTCCGCCCAACAGCAATGGCAACTCAAGTTCCTCCGCCAGCCCGACAACTTCTACTTCAACGAGCTCAAGAAGGGGATTTTTGAATCCAGAACCATAATCAAGGACCTCATCAATGCCGAGCATATCGACGAGGTCTCTATTGTCGATAACGCCACCACTGCCGCCGCCATAGTGCTTCAGAAAATCGCTAGGGATTTTGCGGATGGGAGGTTCGAGAAGGGGGATGCTGCGGTTATGCTTCACTACGCGTATGGTGCTGTGAAGAAGTCCATAGAGGCGTACGTTTCTCGAGCTGGTGGGTATGTTATTGAGGTTCAATTGCCTTTCCCCGTTGAGTCTAATGATGAAATTATATGTGAATTTCGTAAGGCCTTGGAGAGAGGGAAGGCCAATGGTAGGAAGGTTCGTCTGGCTGTGATTGACCATATTACATCCATGCCTTGTGTGGTTATTCCCGTTAAGGAATTGGTTAAGATTTGTAGGGAAGAGGGTGTTGACCAAGTTTTTGTTGATGCTGCTCATGCTATTGGGTGTACTGATATTGATATGCAAGAAATTGGGGCTGATTATTACACTAGTAATTTGCATAAGTGGTTCTTTTGCCCGCCCTCTATTGCTTTCTTGTATTCTAGGAGGTCCCCAAGTCTTTCTGATTTGCATCATCCTGTTGTTTCTCACGAGTACGGTAATGGGTTGCCCATAGAAAGTGCTTGGATTGGAACCAGGGATTATAGTTCTCAATTGGTGGTGCCGTCGGTTTTGAAGTTTGTTAATAGGTTTGAAGATGGGATTAAGGGAATCAAGGAACGGAATCATGAGGCTGTTGTTAAGATGGGAGAGATGTTGGCAGAATCATGGAAGACGCCTCTTGGCTGTCCACCAAATATGTGTGCAAGTATGATCATGGTGGGGCTGCCTTCATGTTTGGGCATCTTGAGTGAATCTGATACTCTGAAGTTGAGGACGCATTTGCGGGAAGAATTTAGAGTCGAAGTCCCAATATATTACCGTGCTCCGAAAAAGGAAGAAATGAGCCCTGTAACAGGATATGCCCGAATTTCTCATCAAGTGTACAATAAATTTGAAGATTATATCAGGTTTAGAGACGCAATCAATGACCTTGTGCGTGCTGGCTTCACTTGTGCTCAACTTTCCAAGTGAGGACTGAGGAGGTATGGTTTCTTTAGCTTCAAAGACTATGGTTGTGTAATTTAGGCAGTTAATATTACAATCGATCTTATAAGGGGAAAAGGAGAATTAAAGTTGCCTAATTCTCTGTTCTTTTGTTTCACTTTGTAAATATACTTGCATTCCATGATTCCAAATGCCAAATTCACACCCGATCCTATGTGAATATCCGATTATAACACTCATTATACTAGTATTGGGCACCTTATTCTTAGATATGTCGCTAATCTTGATGTGATACACATCCCTGATCCTTTACTATGTCTAGTTGACCCACTATGTTTACAGTTAGGTCATTTCCCTATGGCATAATTTCTCATGAATCTACATCCTCCTTATCAGTCATTAAATTTAATAATCATTTTTAATCCTTCCTTAATCCTTCAGATGTCAAGAGGATACTTCGAATGTCATATAAGAACCTACTCTATCATCTTTGTGATAATTATGCAATAATGAATCTGGGTCCATGAGTGGGCAAGTGCTACCATATTCAAATCATGAATATTGCTTTAGAAGGACGTGGATTTTTATGTAGTTCCATTTGTAATTCTACTTATCTAGAGATAAGTGAGCAACTTTTCTTGAATTTTGACTTGGTTTAAGTATGCCCCAAAGAGTTTGTGCCTAATGACAGCGACACAGAGCTTTGACATTGGATCTTTCCATTAGGAGTTTATTCTTCACAGAGTTTGAAGGATTTTATCCATGGATTTGGATATTGTATTGACAGGGAGTAATGTAGAAGGCTCTGTTGTGAGAAGGAACTGTCTCTATTTGTTCGGAATTAGTAGATCTGTTTCTTTTTCAAGAATCAATAGCTTAAGGCATAACTTAGTCATACAGAAATAGAAGCCAAGTTTGGGGTCTACGTCTACTGAAGTCGGTTCAAAGGTTAGACAACTTTAGATGCATCTATTTGGCTATTTCTAACGTACATCAAGTTTTGAGTCTCTTCATGCCATTTTGAGATCCAGTTTGTCCTGCCTGGTTTAAATTTGAACCGGGTTGGTTTATAATAATTGGGGAAGTATATTCTCCCAGTCCCATAATTTAATTAAATCAGATGTGAAATCTAGAAATATTTATTTTGTGTTGGTAGTTGTATTATTTATTTGAATTATTTTTTCCCTAAGGAATTAGGTAGTTGTCAGTCGCAGCAGCTTTTATCCAATTTCTTAAATATGCCTCTATGTTTTTTTTTTGTTTTGCCAGAAAGAATATTAACTGTAGGGGTGAAGAAAGAAGAATAATAGTTCAGCATCTATCTCTTGCTGACCGCTAATAAACTTTTGATGCATAGGAGAGAGAAACGCAGAAAGAGAGAGAACTTTTCTATTTTTTCGAAAAATTAAAGAGCTGTTAAATTTTGAGTGTCGGAGTACGAAGATAGGTACTGACATTCTTCAAGAAAGAAGCCACGTGATACAGTCAAAATTAACTGACTTCTATCTTGAATTGAAATATCAAAGCAACATTAGCCACCTAGGCATTCGAGAGGGTTGGTATTCTCCCTCCCAAGAAATTATATTCTCTAGACCATACCATTACGATTACTAACTACCCTTCAACAGCTTACACGATTATCCTTATATACCTCTATTGTCTAACACACATAATTTTTCTGCATTATATAAGATCTATTTAAGTGCCTACCCTTCCCTTCTAGCTCTACTCTGTAGCTCCACTTGCCTTTTTCCCCATGAAGAACACTTGTGGCTTGGCGTTACCCTTATCTAGGGGTGTAGGGAACCGAACCAAACCAAACCAAACCAAATCAAGCCGTTTTGATTTGTTTCCTCATCATTACTCTTATCAGCTTGTTTACTACTTCAGTGTAGAAACTATTACTTATATTTTAATACAGAAAGTACTTTTGTGAGCCAAAAAGCTCAATTGACTGCTTTGCAAAATAGTTTTTAAAAAATATTCTTAAGGCCTATGATGAGATGACCTGTAGTATTATATATTAGCAAGACTATTGTGTATAGACCCATGATTTTCCAGAGGACACCTTTAGCTATTATGAAAAAACAGACGACGTAGCCATCTTGGGATGACCTAGTGGTCAATAAGGGCCATTGTAAATAGCAAAGGGCTTAGAGGGAATGTTTCATACCATGGTGGCCACCTACCTAGGATTTAATATCCTACGAGTTACCTTGTCAACCAGATGTAGCGAGGTCAGGCTAGTCGAGGTTCACGCAAGCTGGTTCGGACAACGATTTTTTGTTCTTAAAAATACAAATCGGTTTCAAAAAACAACTTTCAGACAGAGCCCCTCATTTTTAAAACAACTTTTTAAAGATACGCTTCTTCTGTCTGGATCGAGCCTGTAAGGTGATGGGAATAAGAATAACTAGGCTTATTGATTTTTCCTAACCATAGATTATGTACCATCTGTATGAATAGTTACATTGAAAAGTGTTTCATATTTGGTTTAGCATTCAGGTCGGTGTCCATGTTGTGATTAAGTTCCCTGTATATAACCGCTCGCTAGCTTTCTCCCGGCTTTTCAATCGATCAAAAGAATATTTCACTATCCACCATGTAATATAGTAAAAAAAGTTTACTCCTGTAGCATAAACTCCTGGTCTTTTCATGTGCTGTGAAGCCTGTGATGAAATGCACAAACATTTATATCTTTCAGTTTGCTACTTCTCTGTGCTAATCATATGTCTTAGACCTAAAGAGCTGCCATTGACATGTAGTTTTTCCATCTCTGCATATACAGCAAGGCTTATGAAAGCGGCACGCTACGGTTCCTCTTCTGCTGCACAGCCATGGTTTCGGTAACCATGGGCTCACCCCCAAAACACTTCAAAGGCGGATGCACCGAGCAGAATAATGTCATTAATCTTTTAATGGCTCAAAAAGTTCCTTAGAGGATAACTTAACCTGTGTATCAGGCTTCATGATCAGCATAGTTCTTGTACATGTTCCAATGTCTTACTGATACTTGGGTGCTTTTGACTCTAATTAACTATCGAATGAAGTACCATTTTCGGTCCAAGTTAAGGCCGTATGATGTAGAGTAGAGTGGACGTTTACTTTAGTGGGTGTCGAGTTGTGGAGTTTCCATGCCCGACGAGTGTGTGAGTTTCCTCCATCCATAATGAAATGACTCTGCAGATTTTGATGAATTGCAGTTCCATATACAGTCCAATCTAATTCCCTACTCACTTTCAGATCTCTTCATTGTTTGGTTTTTTATGAAAGTAATTCAGAAGTTATT

mRNA sequence

ATGGCTTCCCACTGTGAAAAAAATCATCTTCAGCCCGATTCTGCCCCCCATCTCAACGGTGACGCCAATACCCACTTGCCCAAGAAGCCCAAACTCGCCCCCACTTCCATTTCTGATTCCGAAATTCAATCTGAGTTCTCCCACCATGACCCTTCTGTTGCCCGAATCAACAACGGCAGCTTCGGCTGCTGCCCTTCTTCTGTCATCTCCGCCCAACAGCAATGGCAACTCAAGTTCCTCCGCCAGCCCGACAACTTCTACTTCAACGAGCTCAAGAAGGGGATTTTTGAATCCAGAACCATAATCAAGGACCTCATCAATGCCGAGCATATCGACGAGGTCTCTATTGTCGATAACGCCACCACTGCCGCCGCCATAGTGCTTCAGAAAATCGCTAGGGATTTTGCGGATGGGAGGTTCGAGAAGGGGGATGCTGCGGTTATGCTTCACTACGCGTATGGTGCTGTGAAGAAGTCCATAGAGGCGTACGTTTCTCGAGCTGGTGGGTATGTTATTGAGGTTCAATTGCCTTTCCCCGTTGAGTCTAATGATGAAATTATATGTGAATTTCGTAAGGCCTTGGAGAGAGGGAAGGCCAATGGTAGGAAGGTTCGTCTGGCTGTGATTGACCATATTACATCCATGCCTTGTGTGGTTATTCCCGTTAAGGAATTGGTTAAGATTTGTAGGGAAGAGGGTGTTGACCAAGTTTTTGTTGATGCTGCTCATGCTATTGGGTGTACTGATATTGATATGCAAGAAATTGGGGCTGATTATTACACTAGTAATTTGCATAAGTGGTTCTTTTGCCCGCCCTCTATTGCTTTCTTGTATTCTAGGAGGTCCCCAAGTCTTTCTGATTTGCATCATCCTGTTGTTTCTCACGAGTACGGTAATGGGTTGCCCATAGAAAGTGCTTGGATTGGAACCAGGGATTATAGTTCTCAATTGGTGGTGCCGTCGGTTTTGAAGTTTGTTAATAGGTTTGAAGATGGGATTAAGGGAATCAAGGAACGGAATCATGAGGCTGTTGTTAAGATGGGAGAGATGTTGGCAGAATCATGGAAGACGCCTCTTGGCTGTCCACCAAATATGTGTGCAAGTATGATCATGGTGGGGCTGCCTTCATGTTTGGGCATCTTGAGTGAATCTGATACTCTGAAGTTGAGGACGCATTTGCGGGAAGAATTTAGAGTCGAAGTCCCAATATATTACCGTGCTCCGAAAAAGGAAGAAATGAGCCCTGTAACAGGATATGCCCGAATTTCTCATCAAGTGTACAATAAATTTGAAGATTATATCAGGTTTAGAGACGCAATCAATGACCTTGTGCGTGCTGGCTTCACTTGTGCTCAACTTTCCAAGTGAGGACTGAGGAGCAAGGCTTATGAAAGCGGCACGCTACGGTTCCTCTTCTGCTGCACAGCCATGGTTTCGGTAACCATGGGCTCACCCCCAAAACACTTCAAAGGCGGATGCACCGAGCAGAATAATGTCATTAATCTTTTAATGGCTCAAAAAGTTCCTTAGAGGATAACTTAACCTGTGTATCAGGCTTCATGATCAGCATAGTTCTTGTACATGTTCCAATGTCTTACTGATACTTGGGTGCTTTTGACTCTAATTAACTATCGAATGAAGTACCATTTTCGGTCCAAGTTAAGGCCGTATGATGTAGAGTAGAGTGGACGTTTACTTTAGTGGGTGTCGAGTTGTGGAGTTTCCATGCCCGACGAGTGTGTGAGTTTCCTCCATCCATAATGAAATGACTCTGCAGATTTTGATGAATTGCAGTTCCATATACAGTCCAATCTAATTCCCTACTCACTTTCAGATCTCTTCATTGTTTGGTTTTTTATGAAAGTAATTCAGAAGTTATT

Coding sequence (CDS)

ATGGCTTCCCACTGTGAAAAAAATCATCTTCAGCCCGATTCTGCCCCCCATCTCAACGGTGACGCCAATACCCACTTGCCCAAGAAGCCCAAACTCGCCCCCACTTCCATTTCTGATTCCGAAATTCAATCTGAGTTCTCCCACCATGACCCTTCTGTTGCCCGAATCAACAACGGCAGCTTCGGCTGCTGCCCTTCTTCTGTCATCTCCGCCCAACAGCAATGGCAACTCAAGTTCCTCCGCCAGCCCGACAACTTCTACTTCAACGAGCTCAAGAAGGGGATTTTTGAATCCAGAACCATAATCAAGGACCTCATCAATGCCGAGCATATCGACGAGGTCTCTATTGTCGATAACGCCACCACTGCCGCCGCCATAGTGCTTCAGAAAATCGCTAGGGATTTTGCGGATGGGAGGTTCGAGAAGGGGGATGCTGCGGTTATGCTTCACTACGCGTATGGTGCTGTGAAGAAGTCCATAGAGGCGTACGTTTCTCGAGCTGGTGGGTATGTTATTGAGGTTCAATTGCCTTTCCCCGTTGAGTCTAATGATGAAATTATATGTGAATTTCGTAAGGCCTTGGAGAGAGGGAAGGCCAATGGTAGGAAGGTTCGTCTGGCTGTGATTGACCATATTACATCCATGCCTTGTGTGGTTATTCCCGTTAAGGAATTGGTTAAGATTTGTAGGGAAGAGGGTGTTGACCAAGTTTTTGTTGATGCTGCTCATGCTATTGGGTGTACTGATATTGATATGCAAGAAATTGGGGCTGATTATTACACTAGTAATTTGCATAAGTGGTTCTTTTGCCCGCCCTCTATTGCTTTCTTGTATTCTAGGAGGTCCCCAAGTCTTTCTGATTTGCATCATCCTGTTGTTTCTCACGAGTACGGTAATGGGTTGCCCATAGAAAGTGCTTGGATTGGAACCAGGGATTATAGTTCTCAATTGGTGGTGCCGTCGGTTTTGAAGTTTGTTAATAGGTTTGAAGATGGGATTAAGGGAATCAAGGAACGGAATCATGAGGCTGTTGTTAAGATGGGAGAGATGTTGGCAGAATCATGGAAGACGCCTCTTGGCTGTCCACCAAATATGTGTGCAAGTATGATCATGGTGGGGCTGCCTTCATGTTTGGGCATCTTGAGTGAATCTGATACTCTGAAGTTGAGGACGCATTTGCGGGAAGAATTTAGAGTCGAAGTCCCAATATATTACCGTGCTCCGAAAAAGGAAGAAATGAGCCCTGTAACAGGATATGCCCGAATTTCTCATCAAGTGTACAATAAATTTGAAGATTATATCAGGTTTAGAGACGCAATCAATGACCTTGTGCGTGCTGGCTTCACTTGTGCTCAACTTTCCAAGTGA

Protein sequence

MASHCEKNHLQPDSAPHLNGDANTHLPKKPKLAPTSISDSEIQSEFSHHDPSVARINNGSFGCCPSSVISAQQQWQLKFLRQPDNFYFNELKKGIFESRTIIKDLINAEHIDEVSIVDNATTAAAIVLQKIARDFADGRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPVESNDEIICEFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVDAAHAIGCTDIDMQEIGADYYTSNLHKWFFCPPSIAFLYSRRSPSLSDLHHPVVSHEYGNGLPIESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIKERNHEAVVKMGEMLAESWKTPLGCPPNMCASMIMVGLPSCLGILSESDTLKLRTHLREEFRVEVPIYYRAPKKEEMSPVTGYARISHQVYNKFEDYIRFRDAINDLVRAGFTCAQLSK
Homology
BLAST of MC08g2174 vs. ExPASy Swiss-Prot
Match: Q3E6S9 (Probable L-cysteine desulfhydrase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CpNIFS3 PE=1 SV=1)

HSP 1 Score: 657.5 bits (1695), Expect = 1.1e-187
Identity = 316/430 (73.49%), Postives = 368/430 (85.58%), Query Frame = 0

Query: 28  KKPKLA-PTSISDSEIQSEFSHHDPSVARINNGSFGCCPSSVISAQQQWQLKFLRQPDNF 87
           K+PK++ P  IS SEI+SEFSHHDP  ARINNGSFGCCPSS+++ Q+ WQL+FLRQPD F
Sbjct: 45  KRPKISHPNYISSSEIESEFSHHDPDFARINNGSFGCCPSSILALQRDWQLRFLRQPDRF 104

Query: 88  YFNELKKGIFESRTIIKDLINAEHIDEVSIVDNATTAAAIVLQKIARDFADGRFEKGDAA 147
           YF+ELK  I +SR++IK LINAEH DEVSIVDNATTAAAIVLQ+ A  F +GRF+KGDA 
Sbjct: 105 YFDELKPKISDSRSVIKRLINAEHDDEVSIVDNATTAAAIVLQQTAWAFREGRFDKGDAV 164

Query: 148 VMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPVESNDEIICEFRKALERGKANGRKVRL 207
           VMLHYAYG+VKKS+EAYV+R+GG+V EVQLPFPV S DEII  FR  LE GKANGR+VRL
Sbjct: 165 VMLHYAYGSVKKSVEAYVTRSGGHVTEVQLPFPVISADEIIDRFRIGLESGKANGRRVRL 224

Query: 208 AVIDHITSMPCVVIPVKELVKICREEGVDQVFVDAAHAIGCTDIDMQEIGADYYTSNLHK 267
           A+IDH+TSMP VVIP+KELVKICR EGVDQVFVDAAH IGC D+DM+EIGAD+YTSNLHK
Sbjct: 225 ALIDHVTSMPSVVIPIKELVKICRREGVDQVFVDAAHGIGCVDVDMKEIGADFYTSNLHK 284

Query: 268 WFFCPPSIAFLYSRRSPS--LSDLHHPVVSHEYGNGLPIESAWIGTRDYSSQLVVPSVLK 327
           WFF PPS+AFLY R+S +  ++DLHHPVVS+EYGNGL +ES+W+GTRDYS+QLVVPS+L+
Sbjct: 285 WFFAPPSVAFLYCRKSSNGGVADLHHPVVSNEYGNGLAVESSWVGTRDYSAQLVVPSILE 344

Query: 328 FVNRFEDGIKGIKERNHEAVVKMGEMLAESWKTPLGCPPNMCASMIMVGLPSCLGILSES 387
           FVNRFE GI GIK+RNHE+VV+MG+ML +SW T LGCPP MCASMIMVGLP CLG+ SES
Sbjct: 345 FVNRFEGGIDGIKKRNHESVVEMGQMLVKSWGTQLGCPPEMCASMIMVGLPVCLGVSSES 404

Query: 388 DTLKLRTHLREEFRVEVPIYYRAPKKEEMSPVTGYARISHQVYNKFEDYIRFRDAINDLV 447
           D LKLRT LRE+FR+E+PIY+R P   E+ P+TGY RIS QVYNK EDY R RDAIN LV
Sbjct: 405 DVLKLRTFLREKFRIEIPIYFRPPGDGEIDPITGYVRISFQVYNKPEDYHRLRDAINGLV 464

Query: 448 RAGFTCAQLS 455
           R GF C  LS
Sbjct: 465 RDGFKCTSLS 474

BLAST of MC08g2174 vs. ExPASy Swiss-Prot
Match: Q9M1R1 (L-cysteine desulfhydrase OS=Arabidopsis thaliana OX=3702 GN=LCD PE=2 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 1.0e-169
Identity = 284/445 (63.82%), Postives = 356/445 (80.00%), Query Frame = 0

Query: 19  NGDANTH---LPKKPKLAPTSISDSEIQSEFSHHDPSVARINNGSFGCCPSSVISAQQQW 78
           NGD+ +H    PKKP+LA   +++S+I SEF+HH   VARINNGSFGCCP SV+ AQ++W
Sbjct: 8   NGDSMSHNHRAPKKPRLAGL-LTESDIDSEFAHHQTGVARINNGSFGCCPGSVLEAQREW 67

Query: 79  QLKFLRQPDNFYFNELKKGIFESRTIIKDLINAEHIDEVSIVDNATTAAAIVLQKIARDF 138
           QL++LRQPD FYFN L++G+  SRT+I DLINA+ +DEVS+VDNATTAAAIVLQK+ R F
Sbjct: 68  QLRYLRQPDEFYFNGLRRGLLASRTVISDLINADDVDEVSLVDNATTAAAIVLQKVGRCF 127

Query: 139 ADGRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPVESNDEIICEFRKALE 198
           ++G+++K D  VM H A+ +VKKSI+AYVSR GG  +EV+LPFPV SN+EII +FR+ LE
Sbjct: 128 SEGKYKKEDTVVMFHCAFQSVKKSIQAYVSRVGGSTVEVRLPFPVNSNEEIISKFREGLE 187

Query: 199 RGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVDAAHAIGCTDIDMQEI 258
           +G+ANGR VRLA+IDHITSMPCV++PV+ELVKICREEGV+QVFVDAAHAIG   +D++EI
Sbjct: 188 KGRANGRTVRLAIIDHITSMPCVLMPVRELVKICREEGVEQVFVDAAHAIGSVKVDVKEI 247

Query: 259 GADYYTSNLHKWFFCPPSIAFLYSRRSPSLSDLHHPVVSHEYGNGLPIESAWIGTRDYSS 318
           GADYY SNLHKWFFCPPSIAF Y ++  S SD+HHPVVSHE+GNGLPIESAWIGTRDYSS
Sbjct: 248 GADYYVSNLHKWFFCPPSIAFFYCKKRGSESDVHHPVVSHEFGNGLPIESAWIGTRDYSS 307

Query: 319 QLVVPSVLKFVNRFEDGIKGIKERNHEAVVKMGEMLAESWKTPLGCPPNMCASMIMVGLP 378
           QLVVPSV++FVNRFE G++GI  +NH+  V+MG MLA++W T LG PP MC  M+M+GLP
Sbjct: 308 QLVVPSVMEFVNRFEGGMEGIMMKNHDEAVRMGLMLADAWGTNLGSPPEMCVGMVMIGLP 367

Query: 379 SCLGILSESDTLKLRTHLREEFRVEVPIYY-------RAPKKEEMSPVTGYARISHQVYN 438
           S L + S+ D +KLR++LR  + VEVP++Y          K ++   +T Y RISHQVYN
Sbjct: 368 SKLCVGSDEDAIKLRSYLRVHYSVEVPVFYLGLRDGEEGVKDKDSGLITAYVRISHQVYN 427

Query: 439 KFEDYIRFRDAINDLVRAGFTCAQL 454
           K EDY R RDAI +LV+   TC  L
Sbjct: 428 KTEDYERLRDAITELVKDQMTCQNL 451

BLAST of MC08g2174 vs. ExPASy Swiss-Prot
Match: Q9LGZ2 (Putative L-cysteine desulfhydrase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0290100 PE=2 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 7.1e-163
Identity = 281/444 (63.29%), Postives = 345/444 (77.70%), Query Frame = 0

Query: 19  NGDANTHLPKKPKLAPTSISDSEIQSEFSHHDPSVARINNGSFGCCPSSVISAQQQWQLK 78
           NG+     P   K   + IS ++I++EF HH+  VAR+NNGSFGCCPSS++ AQ +WQ  
Sbjct: 29  NGNGPAPRPPPAKRPRSVISAAQIRAEFEHHEAGVARVNNGSFGCCPSSLLDAQARWQRL 88

Query: 79  FLRQPDNFYFNELKKGIFESRTIIKDLINAEHIDEVSIVDNATTAAAIVLQKIARDFADG 138
           F+ QPD+FYF+ L+ G+  SR  +  L+NA  + EVS+VDNATTAAAIVLQ  A  FA+G
Sbjct: 89  FIAQPDDFYFHALQPGLRRSRAAVAGLVNAGDVAEVSLVDNATTAAAIVLQHAAWSFAEG 148

Query: 139 RFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPVESNDEIICEFRKALERGK 198
           RF +GDA +MLHYAYGAVKKSI AYV+RAG  V+EV LPFPV S D II EFR AL+  K
Sbjct: 149 RFSRGDAVLMLHYAYGAVKKSIHAYVARAGATVVEVPLPFPVASADAIIAEFRAALDVAK 208

Query: 199 ANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVDAAHAIGCTDIDMQEIGAD 258
           A GRKVRLAVIDHITSMP VVIPVKELV ICREEGVD+VF+DAAH+IG   +D+++IGAD
Sbjct: 209 AGGRKVRLAVIDHITSMPSVVIPVKELVAICREEGVDKVFIDAAHSIGQVPVDVRDIGAD 268

Query: 259 YYTSNLHKWFFCPPSIAFLYSRR-SPSLSDLHHPVVSHEYGNGLPIESAWIGTRDYSSQL 318
           +YTSNLHKWFFCPP++AFL++R+  P  S LHHPVVSHEYGNGLP+ES WIGTRDYS+QL
Sbjct: 269 FYTSNLHKWFFCPPAVAFLHTRKDDPIASQLHHPVVSHEYGNGLPMESGWIGTRDYSAQL 328

Query: 319 VVPSVLKFVNRFEDGIKGIKERNHEAVVKMGEMLAESWKTPLGCPPNMCASMIMVGLPSC 378
           VVP  + FVNRFE GI+GI+ RNHE V++MG+MLAE+W T LG PP +C SM+MVGLP C
Sbjct: 329 VVPESIDFVNRFEGGIEGIRSRNHEKVIEMGKMLAEAWGTFLGTPPELCGSMVMVGLPGC 388

Query: 379 LGILSESDTLKLRTHLREEFRVEVPIYY--RAPKKEEMS------PVTGYARISHQVYNK 438
           LG+ S+ D +++RT LR++F VEVPIYY  R  + +EM+       VTGY RISHQVYN 
Sbjct: 389 LGVESDDDVMRMRTMLRKDFMVEVPIYYNSRRVEAQEMAKDKNGDAVTGYVRISHQVYNV 448

Query: 439 FEDYIRFRDAINDLVRAGFTCAQL 454
            EDY + RDA+N LV  GFT ++L
Sbjct: 449 TEDYEKLRDAVNKLVADGFTSSKL 472

BLAST of MC08g2174 vs. ExPASy Swiss-Prot
Match: Q5JNT6 (Putative L-cysteine desulfhydrase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0290600 PE=3 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 3.5e-146
Identity = 257/451 (56.98%), Postives = 331/451 (73.39%), Query Frame = 0

Query: 18  LNGDANTHLPKKPK--LAPTSISDSEIQSEFSHHDPSVARINNGSFGCCPSSVISAQQQW 77
           ++G A+    K+P+      +I+D+E+++EF+HHD +VAR+NNG+FGCCP+SV++A+ +W
Sbjct: 19  VDGAASPPSAKRPRAGAGAAAITDAEVRAEFAHHDRAVARLNNGTFGCCPASVLAARARW 78

Query: 78  QLKFLRQPDNFYFNELKKGIFESRTIIKDLINAEHIDEVSIVDNATTAAAIVLQKIARDF 137
           Q  FL QPD FYF+ L+ G+  SR  +   + A    EVS+VDN TTAAAI++Q +A  F
Sbjct: 79  QRLFLSQPDAFYFHHLQPGLARSRAAVAAAVGAGDASEVSLVDNVTTAAAIIMQHVAWSF 138

Query: 138 ADGRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPVESNDEIICEFRKALE 197
           A+G F +GD  +M  Y Y ++K SI AYV+RAG  V+EV LPFPV S D I+ EFR AL 
Sbjct: 139 AEGDFARGDVVLMFLYTYCSIKNSIHAYVARAGATVVEVPLPFPVSSPDAIVAEFRAALA 198

Query: 198 RGKANG-RKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVDAAHAIGCTDIDMQE 257
             +  G R+VRLAVIDHIT+MP V+IPVKELV ICREEGVD+VFVDAAHA+G   +D+++
Sbjct: 199 VARDGGRRRVRLAVIDHITAMPTVLIPVKELVAICREEGVDKVFVDAAHAVGQVPVDVRD 258

Query: 258 IGADYYTSNLHKWFFCPPSIAFLYSRR-SPSLSDLHHPVVSHEYGNGLPIESAWIGTRDY 317
           IGAD+Y SNLHKWFFCP ++AF+++R+  P  S LHHPVVS EYGNGLP+ESAWIG RDY
Sbjct: 259 IGADFYASNLHKWFFCPSAVAFIHTRKDDPVSSKLHHPVVSSEYGNGLPMESAWIGVRDY 318

Query: 318 SSQLVVPSVLKFVNRFEDGIKGIKERNHEAVVKMGEMLAESWKTPLGCPPNMCASMIMVG 377
           S+QLVVP V+ FVNRF+ G++GI+ RNH+ VV+MG MLA +W T LG PP MC SM+MVG
Sbjct: 319 SAQLVVPDVVDFVNRFDGGVEGIRRRNHDKVVEMGTMLAAAWGTFLGTPPEMCGSMLMVG 378

Query: 378 LPSCLGILSESDTLKLRTHLREEFRVEVPIYYR-------AP----KKEEMSPVTGYARI 437
           LP  LG+ SE D + LRT LR++F+VEVP+YY        AP    K     PVTGY RI
Sbjct: 379 LPGSLGVGSEDDAVGLRTMLRKQFKVEVPLYYNSKAAAADAPPEMVKDGNGDPVTGYVRI 438

Query: 438 SHQVYNKFEDYIRFRDAINDLVRAGFTCAQL 454
           SHQVYN  E+Y   RDA+  LV  GFTC +L
Sbjct: 439 SHQVYNVREEYEALRDAVAKLVADGFTCRKL 469

BLAST of MC08g2174 vs. ExPASy Swiss-Prot
Match: A7UX13 (Hercynylcysteine sulfoxide lyase OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=egt-2 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 5.1e-28
Identity = 108/402 (26.87%), Postives = 177/402 (44.03%), Query Frame = 0

Query: 50  DPSVARINNGSFGCCPSSVISAQQQWQLKFLRQPDNFYFNELKKGIFESRTIIKDLINAE 109
           DP+   +N+GSFG  PS++    + +Q     +P  F   +    + ESR  + +L+   
Sbjct: 37  DPAYRNLNHGSFGTIPSAIQQKLRSYQTAAEARPCPFLRYQTPVLLDESRAAVANLLKVP 96

Query: 110 HIDEVSIVDNATTAAAIVLQKIARDFADGRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGG 169
            ++ V  V NAT     VL+ I    ADG+    D  +     YGA  K+I+  +    G
Sbjct: 97  -VETVVFVANATMGVNTVLRNIVWS-ADGK----DEILYFDTIYGACGKTIDYVIEDKRG 156

Query: 170 YVIE--VQLPFPVESNDEIICEFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVK 229
            V    + L +P E +D+++  FR A+++ +  G++ RLAVID ++SMP V  P +++VK
Sbjct: 157 IVSSRCIPLIYPAE-DDDVVAAFRDAIKKSREEGKRPRLAVIDVVSSMPGVRFPFEDIVK 216

Query: 230 ICREEGVDQVFVDAAHAIGCTDIDMQEIGADYYTSNLHKWFFCPPSIAFLYSR------- 289
           IC+EE +    VD A  IG  D+ + E   D+  SN HKW F P   A  Y         
Sbjct: 217 ICKEEEIISC-VDGAQGIGMVDLKITETDPDFLISNCHKWLFTPRGCAVFYVPVRNQHLI 276

Query: 290 RS---------PSLSDLHHPVVSHEYGNGLPIESAWIGTRDYSSQLVVPSVLKFVNRFED 349
           RS         P + +  +P+V     +       ++GT D S    V   +K+      
Sbjct: 277 RSTLPTSHGFVPQVGNRFNPLVPAGNKSAFVSNFEFVGTVDNSPFFCVKDAIKWREEVLG 336

Query: 350 GIKGIKERNHEAVVKMGEMLAESWKTPL---GCPPNMCASMIMVGLPSCLGILSESDTLK 409
           G + I E   +   + G+ +AE   T +        +  +M+ + LP  +G     +  K
Sbjct: 337 GEERIMEYMTKLAREGGQKVAEILGTRVLENSTGTLIRCAMVNIALPFVVG-----EDPK 396

Query: 410 LRTHLREEFRVEVPIYYRAPKKEEMSPVTGYARISHQVYNKF 431
               L E+   +V   Y  P +E          +    +N F
Sbjct: 397 APVKLTEKEEKDVEGLYEIPHEEANMAFKWMYNVLQDEFNTF 425

BLAST of MC08g2174 vs. NCBI nr
Match: XP_022156381.1 (probable L-cysteine desulfhydrase, chloroplastic [Momordica charantia])

HSP 1 Score: 919 bits (2376), Expect = 0.0
Identity = 453/455 (99.56%), Postives = 454/455 (99.78%), Query Frame = 0

Query: 1   MASHCEKNHLQPDSAPHLNGDANTHLPKKPKLAPTSISDSEIQSEFSHHDPSVARINNGS 60
           MASHCEKNHLQPDSAPHLNGDANTHLPKKPKLAPTSISDSEIQSEFSHHDPSVARINNGS
Sbjct: 1   MASHCEKNHLQPDSAPHLNGDANTHLPKKPKLAPTSISDSEIQSEFSHHDPSVARINNGS 60

Query: 61  FGCCPSSVISAQQQWQLKFLRQPDNFYFNELKKGIFESRTIIKDLINAEHIDEVSIVDNA 120
           FGCCPSSVISAQQQWQLKFLRQPDNFYFNELKKGIFESRTIIKDLINAEHIDEVSIVDNA
Sbjct: 61  FGCCPSSVISAQQQWQLKFLRQPDNFYFNELKKGIFESRTIIKDLINAEHIDEVSIVDNA 120

Query: 121 TTAAAIVLQKIARDFADGRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPV 180
           TTAAAIVLQKIARDFADGRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPV
Sbjct: 121 TTAAAIVLQKIARDFADGRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPV 180

Query: 181 ESNDEIICEFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVD 240
           ESNDEIICEFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVD
Sbjct: 181 ESNDEIICEFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVD 240

Query: 241 AAHAIGCTDIDMQEIGADYYTSNLHKWFFCPPSIAFLYSRRSPSLSDLHHPVVSHEYGNG 300
           AAHAIGCTDIDMQEIGADYYTSNLHKWFFCPPSIAFLYSRRSPSLSDLHHPVVSHEYGNG
Sbjct: 241 AAHAIGCTDIDMQEIGADYYTSNLHKWFFCPPSIAFLYSRRSPSLSDLHHPVVSHEYGNG 300

Query: 301 LPIESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIKERNHEAVVKMGEMLAESWKTPLG 360
           LPIESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIK+RNHEAVVKMGEMLA SWKTPLG
Sbjct: 301 LPIESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIKKRNHEAVVKMGEMLAGSWKTPLG 360

Query: 361 CPPNMCASMIMVGLPSCLGILSESDTLKLRTHLREEFRVEVPIYYRAPKKEEMSPVTGYA 420
           CPPNMCASMIMVGLPSCLGILSESDTLKLRTHLREEFRVEVPIYYRAPKKEEMSPVTGYA
Sbjct: 361 CPPNMCASMIMVGLPSCLGILSESDTLKLRTHLREEFRVEVPIYYRAPKKEEMSPVTGYA 420

Query: 421 RISHQVYNKFEDYIRFRDAINDLVRAGFTCAQLSK 455
           RISHQVYNKFEDYIRFRDAINDLVRAGFTCAQLSK
Sbjct: 421 RISHQVYNKFEDYIRFRDAINDLVRAGFTCAQLSK 455

BLAST of MC08g2174 vs. NCBI nr
Match: XP_038890421.1 (probable L-cysteine desulfhydrase, chloroplastic [Benincasa hispida])

HSP 1 Score: 872 bits (2252), Expect = 0.0
Identity = 425/455 (93.41%), Postives = 441/455 (96.92%), Query Frame = 0

Query: 1   MASHCEKNHLQPDSAPHLNGDANTHLPKKPKLAPTSISDSEIQSEFSHHDPSVARINNGS 60
           MASHCEKNHL PDS PHLNGDANTHLPK+PK+APT ISDSEIQSEF+HHDPSVARINNGS
Sbjct: 1   MASHCEKNHLPPDSTPHLNGDANTHLPKRPKIAPTFISDSEIQSEFAHHDPSVARINNGS 60

Query: 61  FGCCPSSVISAQQQWQLKFLRQPDNFYFNELKKGIFESRTIIKDLINAEHIDEVSIVDNA 120
           FGCCPSSVISAQQQWQLKFLRQPD FYFNELKKGI ESRTIIKDLINAEHIDEVSIVDNA
Sbjct: 61  FGCCPSSVISAQQQWQLKFLRQPDWFYFNELKKGILESRTIIKDLINAEHIDEVSIVDNA 120

Query: 121 TTAAAIVLQKIARDFADGRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPV 180
           TTAAAIVLQKIARDFA+GRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPV
Sbjct: 121 TTAAAIVLQKIARDFAEGRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPV 180

Query: 181 ESNDEIICEFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVD 240
           +SNDEI+CEFR+ALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVD
Sbjct: 181 KSNDEIMCEFRQALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVD 240

Query: 241 AAHAIGCTDIDMQEIGADYYTSNLHKWFFCPPSIAFLYSRRSPSLSDLHHPVVSHEYGNG 300
           AAHAIGCTDIDMQEIGADYYTSNLHKWFFCPPSIAFLYSRRSPS SDLHHPVVSHEYGNG
Sbjct: 241 AAHAIGCTDIDMQEIGADYYTSNLHKWFFCPPSIAFLYSRRSPSHSDLHHPVVSHEYGNG 300

Query: 301 LPIESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIKERNHEAVVKMGEMLAESWKTPLG 360
           L IESAWIGTRDYSSQLVVPSVLKFVNRFEDGI GIK+RNHEAV+KMGEMLAESWKT LG
Sbjct: 301 LAIESAWIGTRDYSSQLVVPSVLKFVNRFEDGINGIKKRNHEAVIKMGEMLAESWKTHLG 360

Query: 361 CPPNMCASMIMVGLPSCLGILSESDTLKLRTHLREEFRVEVPIYYRAPKKEEMSPVTGYA 420
           CPPNMCASM+MVGLPSCL I SESDTLKLRTHLREEFRVEVPIYYRAP+K E +P+TGYA
Sbjct: 361 CPPNMCASMVMVGLPSCLDISSESDTLKLRTHLREEFRVEVPIYYRAPRKGETAPITGYA 420

Query: 421 RISHQVYNKFEDYIRFRDAINDLVRAGFTCAQLSK 455
           RISHQVYNKFEDY++FRD IN+LVRAGFTCAQLSK
Sbjct: 421 RISHQVYNKFEDYVKFRDVINELVRAGFTCAQLSK 455

BLAST of MC08g2174 vs. NCBI nr
Match: XP_038896771.1 (probable L-cysteine desulfhydrase, chloroplastic [Benincasa hispida])

HSP 1 Score: 871 bits (2251), Expect = 0.0
Identity = 427/455 (93.85%), Postives = 441/455 (96.92%), Query Frame = 0

Query: 1   MASHCEKNHLQPDSAPHLNGDANTHLPKKPKLAPTSISDSEIQSEFSHHDPSVARINNGS 60
           MASH EKNHL PDS PHLNGDANTHLPK+PK++PTSISDSEIQSEF+HHD SVARINNGS
Sbjct: 1   MASHYEKNHLPPDSTPHLNGDANTHLPKRPKISPTSISDSEIQSEFAHHDLSVARINNGS 60

Query: 61  FGCCPSSVISAQQQWQLKFLRQPDNFYFNELKKGIFESRTIIKDLINAEHIDEVSIVDNA 120
           FGCCPSSVISAQQQWQLKFLRQPD FYFNELKKGI ESRTIIKDLINAEHIDEVSIVDNA
Sbjct: 61  FGCCPSSVISAQQQWQLKFLRQPDYFYFNELKKGILESRTIIKDLINAEHIDEVSIVDNA 120

Query: 121 TTAAAIVLQKIARDFADGRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPV 180
           TTAAAIVLQKIARDFA+GRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPV
Sbjct: 121 TTAAAIVLQKIARDFAEGRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPV 180

Query: 181 ESNDEIICEFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVD 240
           +SNDEIICEFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELV+ICREEGVDQVFVD
Sbjct: 181 KSNDEIICEFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVEICREEGVDQVFVD 240

Query: 241 AAHAIGCTDIDMQEIGADYYTSNLHKWFFCPPSIAFLYSRRSPSLSDLHHPVVSHEYGNG 300
           AAHAIGCTDIDMQEIGADYYTSNLHKWFFCPPSIAFLYSRRSPS SDLHHPVVSHEYGNG
Sbjct: 241 AAHAIGCTDIDMQEIGADYYTSNLHKWFFCPPSIAFLYSRRSPSHSDLHHPVVSHEYGNG 300

Query: 301 LPIESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIKERNHEAVVKMGEMLAESWKTPLG 360
           L IESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIK RNHEAVVKMGEMLAESWKT LG
Sbjct: 301 LAIESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIKRRNHEAVVKMGEMLAESWKTHLG 360

Query: 361 CPPNMCASMIMVGLPSCLGILSESDTLKLRTHLREEFRVEVPIYYRAPKKEEMSPVTGYA 420
           CPPNMCASM+MVGLPSCLGI SESDTLKLRTHLREEFRVEVPIYYRAP+K E +P+TGYA
Sbjct: 361 CPPNMCASMVMVGLPSCLGISSESDTLKLRTHLREEFRVEVPIYYRAPRKGETAPITGYA 420

Query: 421 RISHQVYNKFEDYIRFRDAINDLVRAGFTCAQLSK 455
           RISHQVYNKFEDY++FRD IN+LVRAGFTCAQLSK
Sbjct: 421 RISHQVYNKFEDYVKFRDVINELVRAGFTCAQLSK 455

BLAST of MC08g2174 vs. NCBI nr
Match: XP_008453159.1 (PREDICTED: probable L-cysteine desulfhydrase, chloroplastic [Cucumis melo] >KAA0056087.1 putative L-cysteine desulfhydrase [Cucumis melo var. makuwa] >TYJ96430.1 putative L-cysteine desulfhydrase [Cucumis melo var. makuwa])

HSP 1 Score: 868 bits (2242), Expect = 0.0
Identity = 423/455 (92.97%), Postives = 441/455 (96.92%), Query Frame = 0

Query: 1   MASHCEKNHLQPDSAPHLNGDANTHLPKKPKLAPTSISDSEIQSEFSHHDPSVARINNGS 60
           MASHCEKNHL PDS PHLNGDANTHLPK+PK+APT ISDSEIQSEF+HHDPSVARINNGS
Sbjct: 1   MASHCEKNHLPPDSTPHLNGDANTHLPKRPKIAPTFISDSEIQSEFAHHDPSVARINNGS 60

Query: 61  FGCCPSSVISAQQQWQLKFLRQPDNFYFNELKKGIFESRTIIKDLINAEHIDEVSIVDNA 120
           FGCCPSSVISAQQQWQLKFLRQPD FYFNELKKGI ESRTIIKDLINAEHIDEVSIVDNA
Sbjct: 61  FGCCPSSVISAQQQWQLKFLRQPDWFYFNELKKGILESRTIIKDLINAEHIDEVSIVDNA 120

Query: 121 TTAAAIVLQKIARDFADGRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPV 180
           TTAAAIVLQKIARDF++GRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPV
Sbjct: 121 TTAAAIVLQKIARDFSEGRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPV 180

Query: 181 ESNDEIICEFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVD 240
           +SNDEII EFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVD
Sbjct: 181 KSNDEIIFEFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVD 240

Query: 241 AAHAIGCTDIDMQEIGADYYTSNLHKWFFCPPSIAFLYSRRSPSLSDLHHPVVSHEYGNG 300
           AAHAIGCTDIDMQEIGADYYTSNLHKWFFCPPSIAFLYSRRSP+ SDLHHPVVSHEYGNG
Sbjct: 241 AAHAIGCTDIDMQEIGADYYTSNLHKWFFCPPSIAFLYSRRSPNHSDLHHPVVSHEYGNG 300

Query: 301 LPIESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIKERNHEAVVKMGEMLAESWKTPLG 360
           L IESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIK+RNHEAV+KMGEMLAESWKT LG
Sbjct: 301 LAIESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIKKRNHEAVIKMGEMLAESWKTHLG 360

Query: 361 CPPNMCASMIMVGLPSCLGILSESDTLKLRTHLREEFRVEVPIYYRAPKKEEMSPVTGYA 420
           CPPNMCASM+MVGLPSCLGI SESDTL+LRTHLREEFRVEVPIYYRAPKK E +P+TGYA
Sbjct: 361 CPPNMCASMVMVGLPSCLGISSESDTLRLRTHLREEFRVEVPIYYRAPKKGETAPITGYA 420

Query: 421 RISHQVYNKFEDYIRFRDAINDLVRAGFTCAQLSK 455
           RISHQVYNKFEDY++F+DAIN+LV+ GFTC QLSK
Sbjct: 421 RISHQVYNKFEDYVKFQDAINELVQTGFTCTQLSK 455

BLAST of MC08g2174 vs. NCBI nr
Match: XP_004144738.1 (probable L-cysteine desulfhydrase, chloroplastic [Cucumis sativus] >KGN61027.1 hypothetical protein Csa_021205 [Cucumis sativus])

HSP 1 Score: 862 bits (2226), Expect = 1.18e-314
Identity = 420/455 (92.31%), Postives = 439/455 (96.48%), Query Frame = 0

Query: 1   MASHCEKNHLQPDSAPHLNGDANTHLPKKPKLAPTSISDSEIQSEFSHHDPSVARINNGS 60
           MASHCEKNHL PDS PHLNGDANTHLPK+PK+APT ISDSEIQSEF+HHDPSVARINNGS
Sbjct: 1   MASHCEKNHLPPDSTPHLNGDANTHLPKRPKIAPTFISDSEIQSEFAHHDPSVARINNGS 60

Query: 61  FGCCPSSVISAQQQWQLKFLRQPDNFYFNELKKGIFESRTIIKDLINAEHIDEVSIVDNA 120
           FGCCPSSVISAQQQWQLKFLRQPD FYFN LKKGI ESRTIIKDLINAEHIDEVSIVDNA
Sbjct: 61  FGCCPSSVISAQQQWQLKFLRQPDWFYFNGLKKGILESRTIIKDLINAEHIDEVSIVDNA 120

Query: 121 TTAAAIVLQKIARDFADGRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPV 180
           TTAAAIVLQKIARDF++GRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGG+VIEVQLPFPV
Sbjct: 121 TTAAAIVLQKIARDFSEGRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGHVIEVQLPFPV 180

Query: 181 ESNDEIICEFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVD 240
           +SNDEII EFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQ+FVD
Sbjct: 181 KSNDEIIFEFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQIFVD 240

Query: 241 AAHAIGCTDIDMQEIGADYYTSNLHKWFFCPPSIAFLYSRRSPSLSDLHHPVVSHEYGNG 300
           AAHAIGCTDIDMQEI ADYYTSNLHKWFFCPPSIAFLYSRRSP+ SDLHHPVVSHEYGNG
Sbjct: 241 AAHAIGCTDIDMQEIDADYYTSNLHKWFFCPPSIAFLYSRRSPNHSDLHHPVVSHEYGNG 300

Query: 301 LPIESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIKERNHEAVVKMGEMLAESWKTPLG 360
           L IESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIK+RNHEAV+KMGEMLAESWKT LG
Sbjct: 301 LAIESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIKKRNHEAVIKMGEMLAESWKTHLG 360

Query: 361 CPPNMCASMIMVGLPSCLGILSESDTLKLRTHLREEFRVEVPIYYRAPKKEEMSPVTGYA 420
           CPPNMCASM+MVGLPSCLGI SESDTLKLRTHLREEFRVEVPIYYRAPKK E +P+TGYA
Sbjct: 361 CPPNMCASMVMVGLPSCLGISSESDTLKLRTHLREEFRVEVPIYYRAPKKGETAPITGYA 420

Query: 421 RISHQVYNKFEDYIRFRDAINDLVRAGFTCAQLSK 455
           RISHQVYNKFEDY++F+DAIN+LV+ GFTC QLSK
Sbjct: 421 RISHQVYNKFEDYVKFKDAINELVQTGFTCTQLSK 455

BLAST of MC08g2174 vs. ExPASy TrEMBL
Match: A0A6J1DUT4 (probable L-cysteine desulfhydrase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111023288 PE=4 SV=1)

HSP 1 Score: 919 bits (2376), Expect = 0.0
Identity = 453/455 (99.56%), Postives = 454/455 (99.78%), Query Frame = 0

Query: 1   MASHCEKNHLQPDSAPHLNGDANTHLPKKPKLAPTSISDSEIQSEFSHHDPSVARINNGS 60
           MASHCEKNHLQPDSAPHLNGDANTHLPKKPKLAPTSISDSEIQSEFSHHDPSVARINNGS
Sbjct: 1   MASHCEKNHLQPDSAPHLNGDANTHLPKKPKLAPTSISDSEIQSEFSHHDPSVARINNGS 60

Query: 61  FGCCPSSVISAQQQWQLKFLRQPDNFYFNELKKGIFESRTIIKDLINAEHIDEVSIVDNA 120
           FGCCPSSVISAQQQWQLKFLRQPDNFYFNELKKGIFESRTIIKDLINAEHIDEVSIVDNA
Sbjct: 61  FGCCPSSVISAQQQWQLKFLRQPDNFYFNELKKGIFESRTIIKDLINAEHIDEVSIVDNA 120

Query: 121 TTAAAIVLQKIARDFADGRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPV 180
           TTAAAIVLQKIARDFADGRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPV
Sbjct: 121 TTAAAIVLQKIARDFADGRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPV 180

Query: 181 ESNDEIICEFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVD 240
           ESNDEIICEFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVD
Sbjct: 181 ESNDEIICEFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVD 240

Query: 241 AAHAIGCTDIDMQEIGADYYTSNLHKWFFCPPSIAFLYSRRSPSLSDLHHPVVSHEYGNG 300
           AAHAIGCTDIDMQEIGADYYTSNLHKWFFCPPSIAFLYSRRSPSLSDLHHPVVSHEYGNG
Sbjct: 241 AAHAIGCTDIDMQEIGADYYTSNLHKWFFCPPSIAFLYSRRSPSLSDLHHPVVSHEYGNG 300

Query: 301 LPIESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIKERNHEAVVKMGEMLAESWKTPLG 360
           LPIESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIK+RNHEAVVKMGEMLA SWKTPLG
Sbjct: 301 LPIESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIKKRNHEAVVKMGEMLAGSWKTPLG 360

Query: 361 CPPNMCASMIMVGLPSCLGILSESDTLKLRTHLREEFRVEVPIYYRAPKKEEMSPVTGYA 420
           CPPNMCASMIMVGLPSCLGILSESDTLKLRTHLREEFRVEVPIYYRAPKKEEMSPVTGYA
Sbjct: 361 CPPNMCASMIMVGLPSCLGILSESDTLKLRTHLREEFRVEVPIYYRAPKKEEMSPVTGYA 420

Query: 421 RISHQVYNKFEDYIRFRDAINDLVRAGFTCAQLSK 455
           RISHQVYNKFEDYIRFRDAINDLVRAGFTCAQLSK
Sbjct: 421 RISHQVYNKFEDYIRFRDAINDLVRAGFTCAQLSK 455

BLAST of MC08g2174 vs. ExPASy TrEMBL
Match: A0A5D3B987 (Putative L-cysteine desulfhydrase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G00630 PE=4 SV=1)

HSP 1 Score: 868 bits (2242), Expect = 0.0
Identity = 423/455 (92.97%), Postives = 441/455 (96.92%), Query Frame = 0

Query: 1   MASHCEKNHLQPDSAPHLNGDANTHLPKKPKLAPTSISDSEIQSEFSHHDPSVARINNGS 60
           MASHCEKNHL PDS PHLNGDANTHLPK+PK+APT ISDSEIQSEF+HHDPSVARINNGS
Sbjct: 1   MASHCEKNHLPPDSTPHLNGDANTHLPKRPKIAPTFISDSEIQSEFAHHDPSVARINNGS 60

Query: 61  FGCCPSSVISAQQQWQLKFLRQPDNFYFNELKKGIFESRTIIKDLINAEHIDEVSIVDNA 120
           FGCCPSSVISAQQQWQLKFLRQPD FYFNELKKGI ESRTIIKDLINAEHIDEVSIVDNA
Sbjct: 61  FGCCPSSVISAQQQWQLKFLRQPDWFYFNELKKGILESRTIIKDLINAEHIDEVSIVDNA 120

Query: 121 TTAAAIVLQKIARDFADGRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPV 180
           TTAAAIVLQKIARDF++GRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPV
Sbjct: 121 TTAAAIVLQKIARDFSEGRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPV 180

Query: 181 ESNDEIICEFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVD 240
           +SNDEII EFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVD
Sbjct: 181 KSNDEIIFEFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVD 240

Query: 241 AAHAIGCTDIDMQEIGADYYTSNLHKWFFCPPSIAFLYSRRSPSLSDLHHPVVSHEYGNG 300
           AAHAIGCTDIDMQEIGADYYTSNLHKWFFCPPSIAFLYSRRSP+ SDLHHPVVSHEYGNG
Sbjct: 241 AAHAIGCTDIDMQEIGADYYTSNLHKWFFCPPSIAFLYSRRSPNHSDLHHPVVSHEYGNG 300

Query: 301 LPIESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIKERNHEAVVKMGEMLAESWKTPLG 360
           L IESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIK+RNHEAV+KMGEMLAESWKT LG
Sbjct: 301 LAIESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIKKRNHEAVIKMGEMLAESWKTHLG 360

Query: 361 CPPNMCASMIMVGLPSCLGILSESDTLKLRTHLREEFRVEVPIYYRAPKKEEMSPVTGYA 420
           CPPNMCASM+MVGLPSCLGI SESDTL+LRTHLREEFRVEVPIYYRAPKK E +P+TGYA
Sbjct: 361 CPPNMCASMVMVGLPSCLGISSESDTLRLRTHLREEFRVEVPIYYRAPKKGETAPITGYA 420

Query: 421 RISHQVYNKFEDYIRFRDAINDLVRAGFTCAQLSK 455
           RISHQVYNKFEDY++F+DAIN+LV+ GFTC QLSK
Sbjct: 421 RISHQVYNKFEDYVKFQDAINELVQTGFTCTQLSK 455

BLAST of MC08g2174 vs. ExPASy TrEMBL
Match: A0A1S3BVK0 (probable L-cysteine desulfhydrase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493954 PE=4 SV=1)

HSP 1 Score: 868 bits (2242), Expect = 0.0
Identity = 423/455 (92.97%), Postives = 441/455 (96.92%), Query Frame = 0

Query: 1   MASHCEKNHLQPDSAPHLNGDANTHLPKKPKLAPTSISDSEIQSEFSHHDPSVARINNGS 60
           MASHCEKNHL PDS PHLNGDANTHLPK+PK+APT ISDSEIQSEF+HHDPSVARINNGS
Sbjct: 1   MASHCEKNHLPPDSTPHLNGDANTHLPKRPKIAPTFISDSEIQSEFAHHDPSVARINNGS 60

Query: 61  FGCCPSSVISAQQQWQLKFLRQPDNFYFNELKKGIFESRTIIKDLINAEHIDEVSIVDNA 120
           FGCCPSSVISAQQQWQLKFLRQPD FYFNELKKGI ESRTIIKDLINAEHIDEVSIVDNA
Sbjct: 61  FGCCPSSVISAQQQWQLKFLRQPDWFYFNELKKGILESRTIIKDLINAEHIDEVSIVDNA 120

Query: 121 TTAAAIVLQKIARDFADGRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPV 180
           TTAAAIVLQKIARDF++GRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPV
Sbjct: 121 TTAAAIVLQKIARDFSEGRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPV 180

Query: 181 ESNDEIICEFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVD 240
           +SNDEII EFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVD
Sbjct: 181 KSNDEIIFEFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVD 240

Query: 241 AAHAIGCTDIDMQEIGADYYTSNLHKWFFCPPSIAFLYSRRSPSLSDLHHPVVSHEYGNG 300
           AAHAIGCTDIDMQEIGADYYTSNLHKWFFCPPSIAFLYSRRSP+ SDLHHPVVSHEYGNG
Sbjct: 241 AAHAIGCTDIDMQEIGADYYTSNLHKWFFCPPSIAFLYSRRSPNHSDLHHPVVSHEYGNG 300

Query: 301 LPIESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIKERNHEAVVKMGEMLAESWKTPLG 360
           L IESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIK+RNHEAV+KMGEMLAESWKT LG
Sbjct: 301 LAIESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIKKRNHEAVIKMGEMLAESWKTHLG 360

Query: 361 CPPNMCASMIMVGLPSCLGILSESDTLKLRTHLREEFRVEVPIYYRAPKKEEMSPVTGYA 420
           CPPNMCASM+MVGLPSCLGI SESDTL+LRTHLREEFRVEVPIYYRAPKK E +P+TGYA
Sbjct: 361 CPPNMCASMVMVGLPSCLGISSESDTLRLRTHLREEFRVEVPIYYRAPKKGETAPITGYA 420

Query: 421 RISHQVYNKFEDYIRFRDAINDLVRAGFTCAQLSK 455
           RISHQVYNKFEDY++F+DAIN+LV+ GFTC QLSK
Sbjct: 421 RISHQVYNKFEDYVKFQDAINELVQTGFTCTQLSK 455

BLAST of MC08g2174 vs. ExPASy TrEMBL
Match: A0A0A0LK20 (Aminotran_5 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G034800 PE=4 SV=1)

HSP 1 Score: 862 bits (2226), Expect = 5.72e-315
Identity = 420/455 (92.31%), Postives = 439/455 (96.48%), Query Frame = 0

Query: 1   MASHCEKNHLQPDSAPHLNGDANTHLPKKPKLAPTSISDSEIQSEFSHHDPSVARINNGS 60
           MASHCEKNHL PDS PHLNGDANTHLPK+PK+APT ISDSEIQSEF+HHDPSVARINNGS
Sbjct: 1   MASHCEKNHLPPDSTPHLNGDANTHLPKRPKIAPTFISDSEIQSEFAHHDPSVARINNGS 60

Query: 61  FGCCPSSVISAQQQWQLKFLRQPDNFYFNELKKGIFESRTIIKDLINAEHIDEVSIVDNA 120
           FGCCPSSVISAQQQWQLKFLRQPD FYFN LKKGI ESRTIIKDLINAEHIDEVSIVDNA
Sbjct: 61  FGCCPSSVISAQQQWQLKFLRQPDWFYFNGLKKGILESRTIIKDLINAEHIDEVSIVDNA 120

Query: 121 TTAAAIVLQKIARDFADGRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPV 180
           TTAAAIVLQKIARDF++GRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGG+VIEVQLPFPV
Sbjct: 121 TTAAAIVLQKIARDFSEGRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGHVIEVQLPFPV 180

Query: 181 ESNDEIICEFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVD 240
           +SNDEII EFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQ+FVD
Sbjct: 181 KSNDEIIFEFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQIFVD 240

Query: 241 AAHAIGCTDIDMQEIGADYYTSNLHKWFFCPPSIAFLYSRRSPSLSDLHHPVVSHEYGNG 300
           AAHAIGCTDIDMQEI ADYYTSNLHKWFFCPPSIAFLYSRRSP+ SDLHHPVVSHEYGNG
Sbjct: 241 AAHAIGCTDIDMQEIDADYYTSNLHKWFFCPPSIAFLYSRRSPNHSDLHHPVVSHEYGNG 300

Query: 301 LPIESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIKERNHEAVVKMGEMLAESWKTPLG 360
           L IESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIK+RNHEAV+KMGEMLAESWKT LG
Sbjct: 301 LAIESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIKKRNHEAVIKMGEMLAESWKTHLG 360

Query: 361 CPPNMCASMIMVGLPSCLGILSESDTLKLRTHLREEFRVEVPIYYRAPKKEEMSPVTGYA 420
           CPPNMCASM+MVGLPSCLGI SESDTLKLRTHLREEFRVEVPIYYRAPKK E +P+TGYA
Sbjct: 361 CPPNMCASMVMVGLPSCLGISSESDTLKLRTHLREEFRVEVPIYYRAPKKGETAPITGYA 420

Query: 421 RISHQVYNKFEDYIRFRDAINDLVRAGFTCAQLSK 455
           RISHQVYNKFEDY++F+DAIN+LV+ GFTC QLSK
Sbjct: 421 RISHQVYNKFEDYVKFKDAINELVQTGFTCTQLSK 455

BLAST of MC08g2174 vs. ExPASy TrEMBL
Match: A0A6J1FCN9 (probable L-cysteine desulfhydrase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111444191 PE=4 SV=1)

HSP 1 Score: 855 bits (2210), Expect = 1.64e-312
Identity = 417/455 (91.65%), Postives = 438/455 (96.26%), Query Frame = 0

Query: 1   MASHCEKNHLQPDSAPHLNGDANTHLPKKPKLAPTSISDSEIQSEFSHHDPSVARINNGS 60
           MASHC+K++L+PDS PHLNGD +THLPKKPK+APTSISDSEIQSEF+HHD SVARINNGS
Sbjct: 1   MASHCQKSYLRPDSTPHLNGDTDTHLPKKPKIAPTSISDSEIQSEFAHHDLSVARINNGS 60

Query: 61  FGCCPSSVISAQQQWQLKFLRQPDNFYFNELKKGIFESRTIIKDLINAEHIDEVSIVDNA 120
           FGCCPSSVISAQQQWQLKFLRQPD FYFNELKKGI ESRTIIKDLINAEHIDEVSIVDNA
Sbjct: 61  FGCCPSSVISAQQQWQLKFLRQPDYFYFNELKKGILESRTIIKDLINAEHIDEVSIVDNA 120

Query: 121 TTAAAIVLQKIARDFADGRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPV 180
           TTAAAIVLQKIARDFA+GRF+KGDAAVMLHYAYGAVKKS+EAYVSRAG YVIEVQLPFPV
Sbjct: 121 TTAAAIVLQKIARDFAEGRFQKGDAAVMLHYAYGAVKKSVEAYVSRAGAYVIEVQLPFPV 180

Query: 181 ESNDEIICEFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVD 240
           ESNDEIICEFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVD
Sbjct: 181 ESNDEIICEFRKALERGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVD 240

Query: 241 AAHAIGCTDIDMQEIGADYYTSNLHKWFFCPPSIAFLYSRRSPSLSDLHHPVVSHEYGNG 300
           AAHAIGCTDI+MQEIGADYYTSNLHKWFFCPPSIAFLYSRRSPSLSDLHHPVVSHEYGNG
Sbjct: 241 AAHAIGCTDINMQEIGADYYTSNLHKWFFCPPSIAFLYSRRSPSLSDLHHPVVSHEYGNG 300

Query: 301 LPIESAWIGTRDYSSQLVVPSVLKFVNRFEDGIKGIKERNHEAVVKMGEMLAESWKTPLG 360
           L IESAWIGTRDYSSQLVVPSVLKFVNRFEDGI+GIK+RNHEAVVKMGEML ESWKT LG
Sbjct: 301 LAIESAWIGTRDYSSQLVVPSVLKFVNRFEDGIEGIKKRNHEAVVKMGEMLVESWKTRLG 360

Query: 361 CPPNMCASMIMVGLPSCLGILSESDTLKLRTHLREEFRVEVPIYYRAPKKEEMSPVTGYA 420
           CPP+MCASMIMVGLPSCLGI SESDT+ LRTHLREEFRVEVPIYYRAP+K E   +TGYA
Sbjct: 361 CPPSMCASMIMVGLPSCLGISSESDTMMLRTHLREEFRVEVPIYYRAPRKGETGTITGYA 420

Query: 421 RISHQVYNKFEDYIRFRDAINDLVRAGFTCAQLSK 455
           RISHQVYNKFEDY++ RD IN+LVRAGFTCA+LSK
Sbjct: 421 RISHQVYNKFEDYVKLRDGINELVRAGFTCAELSK 455

BLAST of MC08g2174 vs. TAIR 10
Match: AT5G26600.2 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 657.5 bits (1695), Expect = 7.7e-189
Identity = 316/430 (73.49%), Postives = 368/430 (85.58%), Query Frame = 0

Query: 28  KKPKLA-PTSISDSEIQSEFSHHDPSVARINNGSFGCCPSSVISAQQQWQLKFLRQPDNF 87
           K+PK++ P  IS SEI+SEFSHHDP  ARINNGSFGCCPSS+++ Q+ WQL+FLRQPD F
Sbjct: 45  KRPKISHPNYISSSEIESEFSHHDPDFARINNGSFGCCPSSILALQRDWQLRFLRQPDRF 104

Query: 88  YFNELKKGIFESRTIIKDLINAEHIDEVSIVDNATTAAAIVLQKIARDFADGRFEKGDAA 147
           YF+ELK  I +SR++IK LINAEH DEVSIVDNATTAAAIVLQ+ A  F +GRF+KGDA 
Sbjct: 105 YFDELKPKISDSRSVIKRLINAEHDDEVSIVDNATTAAAIVLQQTAWAFREGRFDKGDAV 164

Query: 148 VMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPVESNDEIICEFRKALERGKANGRKVRL 207
           VMLHYAYG+VKKS+EAYV+R+GG+V EVQLPFPV S DEII  FR  LE GKANGR+VRL
Sbjct: 165 VMLHYAYGSVKKSVEAYVTRSGGHVTEVQLPFPVISADEIIDRFRIGLESGKANGRRVRL 224

Query: 208 AVIDHITSMPCVVIPVKELVKICREEGVDQVFVDAAHAIGCTDIDMQEIGADYYTSNLHK 267
           A+IDH+TSMP VVIP+KELVKICR EGVDQVFVDAAH IGC D+DM+EIGAD+YTSNLHK
Sbjct: 225 ALIDHVTSMPSVVIPIKELVKICRREGVDQVFVDAAHGIGCVDVDMKEIGADFYTSNLHK 284

Query: 268 WFFCPPSIAFLYSRRSPS--LSDLHHPVVSHEYGNGLPIESAWIGTRDYSSQLVVPSVLK 327
           WFF PPS+AFLY R+S +  ++DLHHPVVS+EYGNGL +ES+W+GTRDYS+QLVVPS+L+
Sbjct: 285 WFFAPPSVAFLYCRKSSNGGVADLHHPVVSNEYGNGLAVESSWVGTRDYSAQLVVPSILE 344

Query: 328 FVNRFEDGIKGIKERNHEAVVKMGEMLAESWKTPLGCPPNMCASMIMVGLPSCLGILSES 387
           FVNRFE GI GIK+RNHE+VV+MG+ML +SW T LGCPP MCASMIMVGLP CLG+ SES
Sbjct: 345 FVNRFEGGIDGIKKRNHESVVEMGQMLVKSWGTQLGCPPEMCASMIMVGLPVCLGVSSES 404

Query: 388 DTLKLRTHLREEFRVEVPIYYRAPKKEEMSPVTGYARISHQVYNKFEDYIRFRDAINDLV 447
           D LKLRT LRE+FR+E+PIY+R P   E+ P+TGY RIS QVYNK EDY R RDAIN LV
Sbjct: 405 DVLKLRTFLREKFRIEIPIYFRPPGDGEIDPITGYVRISFQVYNKPEDYHRLRDAINGLV 464

Query: 448 RAGFTCAQLS 455
           R GF C  LS
Sbjct: 465 RDGFKCTSLS 474

BLAST of MC08g2174 vs. TAIR 10
Match: AT5G26600.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 657.5 bits (1695), Expect = 7.7e-189
Identity = 316/430 (73.49%), Postives = 368/430 (85.58%), Query Frame = 0

Query: 28  KKPKLA-PTSISDSEIQSEFSHHDPSVARINNGSFGCCPSSVISAQQQWQLKFLRQPDNF 87
           K+PK++ P  IS SEI+SEFSHHDP  ARINNGSFGCCPSS+++ Q+ WQL+FLRQPD F
Sbjct: 45  KRPKISHPNYISSSEIESEFSHHDPDFARINNGSFGCCPSSILALQRDWQLRFLRQPDRF 104

Query: 88  YFNELKKGIFESRTIIKDLINAEHIDEVSIVDNATTAAAIVLQKIARDFADGRFEKGDAA 147
           YF+ELK  I +SR++IK LINAEH DEVSIVDNATTAAAIVLQ+ A  F +GRF+KGDA 
Sbjct: 105 YFDELKPKISDSRSVIKRLINAEHDDEVSIVDNATTAAAIVLQQTAWAFREGRFDKGDAV 164

Query: 148 VMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPVESNDEIICEFRKALERGKANGRKVRL 207
           VMLHYAYG+VKKS+EAYV+R+GG+V EVQLPFPV S DEII  FR  LE GKANGR+VRL
Sbjct: 165 VMLHYAYGSVKKSVEAYVTRSGGHVTEVQLPFPVISADEIIDRFRIGLESGKANGRRVRL 224

Query: 208 AVIDHITSMPCVVIPVKELVKICREEGVDQVFVDAAHAIGCTDIDMQEIGADYYTSNLHK 267
           A+IDH+TSMP VVIP+KELVKICR EGVDQVFVDAAH IGC D+DM+EIGAD+YTSNLHK
Sbjct: 225 ALIDHVTSMPSVVIPIKELVKICRREGVDQVFVDAAHGIGCVDVDMKEIGADFYTSNLHK 284

Query: 268 WFFCPPSIAFLYSRRSPS--LSDLHHPVVSHEYGNGLPIESAWIGTRDYSSQLVVPSVLK 327
           WFF PPS+AFLY R+S +  ++DLHHPVVS+EYGNGL +ES+W+GTRDYS+QLVVPS+L+
Sbjct: 285 WFFAPPSVAFLYCRKSSNGGVADLHHPVVSNEYGNGLAVESSWVGTRDYSAQLVVPSILE 344

Query: 328 FVNRFEDGIKGIKERNHEAVVKMGEMLAESWKTPLGCPPNMCASMIMVGLPSCLGILSES 387
           FVNRFE GI GIK+RNHE+VV+MG+ML +SW T LGCPP MCASMIMVGLP CLG+ SES
Sbjct: 345 FVNRFEGGIDGIKKRNHESVVEMGQMLVKSWGTQLGCPPEMCASMIMVGLPVCLGVSSES 404

Query: 388 DTLKLRTHLREEFRVEVPIYYRAPKKEEMSPVTGYARISHQVYNKFEDYIRFRDAINDLV 447
           D LKLRT LRE+FR+E+PIY+R P   E+ P+TGY RIS QVYNK EDY R RDAIN LV
Sbjct: 405 DVLKLRTFLREKFRIEIPIYFRPPGDGEIDPITGYVRISFQVYNKPEDYHRLRDAINGLV 464

Query: 448 RAGFTCAQLS 455
           R GF C  LS
Sbjct: 465 RDGFKCTSLS 474

BLAST of MC08g2174 vs. TAIR 10
Match: AT3G62130.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 597.8 bits (1540), Expect = 7.2e-171
Identity = 284/445 (63.82%), Postives = 356/445 (80.00%), Query Frame = 0

Query: 19  NGDANTH---LPKKPKLAPTSISDSEIQSEFSHHDPSVARINNGSFGCCPSSVISAQQQW 78
           NGD+ +H    PKKP+LA   +++S+I SEF+HH   VARINNGSFGCCP SV+ AQ++W
Sbjct: 8   NGDSMSHNHRAPKKPRLAGL-LTESDIDSEFAHHQTGVARINNGSFGCCPGSVLEAQREW 67

Query: 79  QLKFLRQPDNFYFNELKKGIFESRTIIKDLINAEHIDEVSIVDNATTAAAIVLQKIARDF 138
           QL++LRQPD FYFN L++G+  SRT+I DLINA+ +DEVS+VDNATTAAAIVLQK+ R F
Sbjct: 68  QLRYLRQPDEFYFNGLRRGLLASRTVISDLINADDVDEVSLVDNATTAAAIVLQKVGRCF 127

Query: 139 ADGRFEKGDAAVMLHYAYGAVKKSIEAYVSRAGGYVIEVQLPFPVESNDEIICEFRKALE 198
           ++G+++K D  VM H A+ +VKKSI+AYVSR GG  +EV+LPFPV SN+EII +FR+ LE
Sbjct: 128 SEGKYKKEDTVVMFHCAFQSVKKSIQAYVSRVGGSTVEVRLPFPVNSNEEIISKFREGLE 187

Query: 199 RGKANGRKVRLAVIDHITSMPCVVIPVKELVKICREEGVDQVFVDAAHAIGCTDIDMQEI 258
           +G+ANGR VRLA+IDHITSMPCV++PV+ELVKICREEGV+QVFVDAAHAIG   +D++EI
Sbjct: 188 KGRANGRTVRLAIIDHITSMPCVLMPVRELVKICREEGVEQVFVDAAHAIGSVKVDVKEI 247

Query: 259 GADYYTSNLHKWFFCPPSIAFLYSRRSPSLSDLHHPVVSHEYGNGLPIESAWIGTRDYSS 318
           GADYY SNLHKWFFCPPSIAF Y ++  S SD+HHPVVSHE+GNGLPIESAWIGTRDYSS
Sbjct: 248 GADYYVSNLHKWFFCPPSIAFFYCKKRGSESDVHHPVVSHEFGNGLPIESAWIGTRDYSS 307

Query: 319 QLVVPSVLKFVNRFEDGIKGIKERNHEAVVKMGEMLAESWKTPLGCPPNMCASMIMVGLP 378
           QLVVPSV++FVNRFE G++GI  +NH+  V+MG MLA++W T LG PP MC  M+M+GLP
Sbjct: 308 QLVVPSVMEFVNRFEGGMEGIMMKNHDEAVRMGLMLADAWGTNLGSPPEMCVGMVMIGLP 367

Query: 379 SCLGILSESDTLKLRTHLREEFRVEVPIYY-------RAPKKEEMSPVTGYARISHQVYN 438
           S L + S+ D +KLR++LR  + VEVP++Y          K ++   +T Y RISHQVYN
Sbjct: 368 SKLCVGSDEDAIKLRSYLRVHYSVEVPVFYLGLRDGEEGVKDKDSGLITAYVRISHQVYN 427

Query: 439 KFEDYIRFRDAINDLVRAGFTCAQL 454
           K EDY R RDAI +LV+   TC  L
Sbjct: 428 KTEDYERLRDAITELVKDQMTCQNL 451

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3E6S91.1e-18773.49Probable L-cysteine desulfhydrase, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q9M1R11.0e-16963.82L-cysteine desulfhydrase OS=Arabidopsis thaliana OX=3702 GN=LCD PE=2 SV=1[more]
Q9LGZ27.1e-16363.29Putative L-cysteine desulfhydrase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q5JNT63.5e-14656.98Putative L-cysteine desulfhydrase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
A7UX135.1e-2826.87Hercynylcysteine sulfoxide lyase OS=Neurospora crassa (strain ATCC 24698 / 74-OR... [more]
Match NameE-valueIdentityDescription
XP_022156381.10.099.56probable L-cysteine desulfhydrase, chloroplastic [Momordica charantia][more]
XP_038890421.10.093.41probable L-cysteine desulfhydrase, chloroplastic [Benincasa hispida][more]
XP_038896771.10.093.85probable L-cysteine desulfhydrase, chloroplastic [Benincasa hispida][more]
XP_008453159.10.092.97PREDICTED: probable L-cysteine desulfhydrase, chloroplastic [Cucumis melo] >KAA0... [more]
XP_004144738.11.18e-31492.31probable L-cysteine desulfhydrase, chloroplastic [Cucumis sativus] >KGN61027.1 h... [more]
Match NameE-valueIdentityDescription
A0A6J1DUT40.099.56probable L-cysteine desulfhydrase, chloroplastic OS=Momordica charantia OX=3673 ... [more]
A0A5D3B9870.092.97Putative L-cysteine desulfhydrase OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3BVK00.092.97probable L-cysteine desulfhydrase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A0A0LK205.72e-31592.31Aminotran_5 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G034800... [more]
A0A6J1FCN91.64e-31291.65probable L-cysteine desulfhydrase, chloroplastic OS=Cucurbita moschata OX=3662 G... [more]
Match NameE-valueIdentityDescription
AT5G26600.27.7e-18973.49Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
AT5G26600.17.7e-18973.49Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
AT3G62130.17.2e-17163.82Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 66..327
e-value: 1.8E-38
score: 134.1
IPR000192Aminotransferase class V domainPFAMPF00266Aminotran_5coord: 90..282
e-value: 7.6E-16
score: 58.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..38
NoneNo IPR availablePANTHERPTHR43092L-CYSTEINE DESULFHYDRASEcoord: 17..454
NoneNo IPR availablePANTHERPTHR43092:SF7L-CYSTEINE DESULFHYDRASEcoord: 17..454
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 46..445

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g2174.1MC08g2174.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003824 catalytic activity