MC08g2148 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g2148
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Translation initiation factor IF-3
Location	MC08: 30440291 .. 30444496 (+)
RNA-Seq Expression	MC08g2148
Synteny	MC08g2148

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCGTTATAATGATGATAAGGAAGTAGAAAATGAGCTATAACAAAAGGCAACTAAAATGATTTTTCCGAAAAAAAAAATTAATAATAATAGAGTCCACTCCACAGTCCAAAATTATTGTGGATAAGATTTTTAATTTTCTTTTTCAATTTCTTGAAAAATTTTGGCCAAACTTCCACCATTCCACTGCCCCTTCAACAGCTCAAAGCTATGGCTGGGCTCACCAGCAGATTCCCTTTCAAACCTCTCTTATCCCAATCCTCCGCCACCACCTCCTCCGCCGCCGCCTCCTCTTCCTCCTCCGCCGCCGCCGCTCCTCCCTTCCGAACCTCAATTTCCCCTCTCCCTCTCTCCTCCTCTGAATCCAATGCCTTCGGATTCCGCCTTCACAACTCCAATTCTCTGCGTCTTATCGTCTCCTCTCCAATTCGTCGCTCTGTATTTGCTCGATTCGGCGGTGCTCCTCGATCTTCAGGACCTGCGGATTCTAAGCGCTCCGACGATGATTTTGGTCTCGATGTTTCCGCCATCAGGTGCGTGCCTTATGGTTCTGTATGTGTACTTGCACGAGTTACGTGTATGGTGTATGAAGCTTGTTTTTGATTTGGAGTTCTTATTTGCGATTGATTGGTATAGGTCAAATAATGTGAGACTTATTGATGCACAACAGAACATGGTAGGTGTTTTTAATTATTGTTTGTTTGTTTTGTCTGGATGGGTTGTGTTTGGGATAAACGAGTTTTTTGGGTTTCTTTTGCTACAATGCAACACCTGTATGCCAGGCGCCGTAATTTGGAAGTTGTTCTTGAAATGAATAGGTTGGAATCGTGTCAAAGGCCCAGGCCATTCAAATGGCTGAAGACGCTGAACTTGATTTGGTACGTCTTCCTGTTCAATTTTTTGTTGAAACAGTTTTCTATTCTTTGTCAGGGAATTCCAAGGAACCTTGCTCCAAATTTTTATGGTCTGACAATGACAAATTGCATTGTTAATCTTTCTTGGTCGTCTGAATTCTGGATTTTGTTTGCTTAATGTCACTCAGTTTTTGGTTTTTATCTTCAATACAATTGTTCTGTGTAGAAAAAATGAGCATATAATTGAAAGCTTATAATAGTATTATAATCAAAGAATTATATTATAACTTATAAGTTACAAGTCTTCCATCAAAAACTCGAAGAATTATTGCATGGTGAATGATTTTCATTTGAGAGTTATGCATTAGGTAGGCCAATTTTACTGTTTGCTCCTATATATAATTGTACTTTTCTCCATAAATCGTCAAAAGAACCTTCTATTCTTCAACACAAATCTTATCGACTGAAAATTTACATGGACGAAAGCTGGGCGATGTGTTGTTATTTAGATATGAAGGAAGAAACTTCATTCTTCATGCGAGTTCTTAACAAAATACTCTTTCAGAAATCAACAAGTTAGTTGTCTTGCATACTCAAGTTTGGCTTGTGGAATTTATGAAGCTCATTTCATATCCGTGCAATTTGTACTTCTTAAGCTGGATAAAGAAAGTTGGACTAATATTTTTTTTTATGGAAGTTAGATATTTGTGTTGCTTTGTCTAGGAGTTTGCTAATTCGTTTCTATTTCATGCAGGTTGTATTATCACCCGATGCAGATCCTCCAGTTGTCAGAATAATGGATTACAAGTATGCCATTTTCTCTCTCTGGTGTCCTCCCTTTCCTTGTTGTGCTTGTGGAAAAGGTTTCATTTGCTGCAATCCAATGCCAATGTGATTAGTTTTAATGATTATGCAAATTCATATAATAAGCTTCTTCTGGTAATGATTCAATTAATACTTTATTTTTAATTAAATGTTACCTAAAAGGTAATATATGACAATGCTAAGAAGACCGAAATGTGGTATAATGGAGAAAGAATGAAAAATATGATGATCGTTATTCTTCCACTGGGTGAATTAAAAGCCCATGTGGTTTTTCCTTACTGAATTGTTGGTACCTTTATGCTGAGCTATTCTTCCGTCTCTTGTTTCTCAGTAAATATAGGTATGAATTGCAAAAGAAGAAAAGAGGACAGCAGAAGAAAAGTGCTGGTGAATCTCTTTCTTAATAGAGTCTGCATGTTTTCTTTGGTTCCCAAATCTTTCAGATTTGAGGAAAGATACTGATAAAGTGTGCTTTTGAATGTTAGCAACTCGCATGGATTTGAAAGAGCTTAAAATGGGGTAACACATTCCTTCATTTGTTCATATTTATTTTTTCGAAGGCTTCCTTTCTCTTCTTCTTATTCACCTAAAGCCACTCGTACTTGTGACTTCTATGATCGATAATCTACCGACCAATCAACAAGCTAAGAGAGGCTTTGGTTCCTTTGCTTTTTAATTTATGTTACCTATTTCAGAAACATTTTTGTACATTATCCTTTCCTCAATCTCAACCTGTTTGTTGATATTATTATTGCACAGTTATAACATTGACCAACATGATTACTCTGTTCGTTTGAAAGCTGCAAGGAGGTTTTTGAAGGATGGGGACAAGGTTTTATTTTTTATTTAACTATATCTATGTGTGTGTATATATATGTATATATATATATATATATATTGTCTGTCCATGTATTCAAATATGATTTTTATGGTTTGTCCGAATCACTTTCTGAAATATATTAACAAAATATTGCAGGTTAAAGTTATAGTGAACTTGAAAGGTCGTGAGAATGAGTTCAGAAATATAGCAATTGAGCTCATCAGACGTTTCCAGGATGAGATTGGGGAGGTATTCTTATTTTCTATCAATTTTGTGCCATGATCGATCTCCTGACTTAGAGCAGTTTTTGTCCATTATGGCAAATATTAGAGCAAAACTTTTTTTTTTGGGTAAGAAACGATCATTTCATTGATGAATGAAATTACAAAAGAAGCATCCCCGAAATGGAATATGCTGACTTTTCGAAACGATGATTTCATCCTGACTTTCTTTTTGATGTCATATGTGTTAGAGCAAAACATATTGCCCATCATGGTAGTATACTCAATAACATGCTACAAAACTTTTCAGAGTTTGAAATTTGAAATGGTAATTTTAGGCAATTGTTTGAACTATTTTTATGTTTGATCTCATTACCCCGTGATAAGCACTTGGTAGTTGTTCCAGAAATGGTTATGGCAAACAGTTTCTGAAGACTTTTATAGAACACTTGTTTAAAATAATTGTGAATGCTCGGCTAACTTACGTGAATCTCGACTAATCTCACAAAATAATCTACTTGTTCCTGTGATATTAGATGTTGGAAAATCGAACTCATTTCTCAAGAGTACTTCTGTGTTTTCACTTGCTCCAATGAGCATTAAGCCAATTCATGGTAGTTTAATTGAAAGGTTTTTGAAAAATGCTTGTTTGTATTTGTGAAGTTTTTTCTTATTTGGATTCGATAATCTCTCTGAAATGCTAGTTTATTTGATGAATGAAAATTTGATGCCATCTTTTTTCCTCTGGTTAATATGTTAAACAAAGACAATCTATCGTATATTAATCTCTGTTCTCCCATGACCCGCTCAATATTTTATGATACCACAGCTTGCAACTGAAGAGGCCAAGAACTTCAGAGACAGGAATATATTTATTGTTCTAGTTCCTAACAAGGCTGTTCTACAGAAGGCTCAAGAACCCCCCAAGAAAAAGGACAATCCCGCCATAAATGAAGTTTCGGCTGGTGTTTAAGCTCGGAAAGCGTCGAGCTTGTGCCTAAAATAGAACGGTTAGTCGCAACAACCTTAGTCTTCTTTATCATAGAGAAATCAGAGAGGTTAATGTATAATGTTTTGTTTAATCTTATGAATCAGACTGTTGTTCATCTTATTTATTTAGTGGAATGCTTAGAAATCAGAGTTGTTGTTACTAATCCTTTTGAAAACTTGAACTTTTGTAGCATCAGTTTTTCTGGGTAGGGAATGGAGATGTTATTTTCTCCGCGGGGACGCCCATAACAGGGCGAGCTTCCGTGAGGCGGCAGTGAGGGACATTGTCCTTGTCACCTAAATGCGGACGGGCAATACGTACTCTATCCCGCCCCATTCTTGATGTTGTTGACATTTTATCAATATTTCTTGACATCTATATTTTACCCTTTGTCGACAATATTTTCTTAATAAGACGATATTTTACCCTTCGTTGAGGTGTCGAACCCTCTGACATCAATGTTGCTTGACATTTTAAACACCACTTATAAATCAATC

mRNA sequence

CTCGTTATAATGATGATAAGGAAGTAGAAAATGAGCTATAACAAAAGGCAACTAAAATGATTTTTCCGAAAAAAAAAATTAATAATAATAGAGTCCACTCCACAGTCCAAAATTATTGTGGATAAGATTTTTAATTTTCTTTTTCAATTTCTTGAAAAATTTTGGCCAAACTTCCACCATTCCACTGCCCCTTCAACAGCTCAAAGCTATGGCTGGGCTCACCAGCAGATTCCCTTTCAAACCTCTCTTATCCCAATCCTCCGCCACCACCTCCTCCGCCGCCGCCTCCTCTTCCTCCTCCGCCGCCGCCGCTCCTCCCTTCCGAACCTCAATTTCCCCTCTCCCTCTCTCCTCCTCTGAATCCAATGCCTTCGGATTCCGCCTTCACAACTCCAATTCTCTGCGTCTTATCGTCTCCTCTCCAATTCGTCGCTCTGTATTTGCTCGATTCGGCGGTGCTCCTCGATCTTCAGGACCTGCGGATTCTAAGCGCTCCGACGATGATTTTGGTCTCGATGTTTCCGCCATCAGGTCAAATAATGTGAGACTTATTGATGCACAACAGAACATGGTTGGAATCGTGTCAAAGGCCCAGGCCATTCAAATGGCTGAAGACGCTGAACTTGATTTGGTTGTATTATCACCCGATGCAGATCCTCCAGTTGTCAGAATAATGGATTACAATAAATATAGGTATGAATTGCAAAAGAAGAAAAGAGGACAGCAGAAGAAAAGTGCTGCAACTCGCATGGATTTGAAAGAGCTTAAAATGGGTTATAACATTGACCAACATGATTACTCTGTTCGTTTGAAAGCTGCAAGGAGGTTTTTGAAGGATGGGGACAAGGTTAAAGTTATAGTGAACTTGAAAGGTCGTGAGAATGAGTTCAGAAATATAGCAATTGAGCTCATCAGACGTTTCCAGGATGAGATTGGGGAGCTTGCAACTGAAGAGGCCAAGAACTTCAGAGACAGGAATATATTTATTGTTCTAGTTCCTAACAAGGCTGTTCTACAGAAGGCTCAAGAACCCCCCAAGAAAAAGGACAATCCCGCCATAAATGAAGTTTCGGCTGGTGTTTAAGCTCGGAAAGCGTCGAGCTTGTGCCTAAAATAGAACGCATCAGTTTTTCTGGGTAGGGAATGGAGATGTTATTTTCTCCGCGGGGACGCCCATAACAGGGCGAGCTTCCGTGAGGCGGCAGTGAGGGACATTGTCCTTGTCACCTAAATGCGGACGGGCAATACGTACTCTATCCCGCCCCATTCTTGATGTTGTTGACATTTTATCAATATTTCTTGACATCTATATTTTACCCTTTGTCGACAATATTTTCTTAATAAGACGATATTTTACCCTTCGTTGAGGTGTCGAACCCTCTGACATCAATGTTGCTTGACATTTTAAACACCACTTATAAATCAATC

Coding sequence (CDS)

ATGGCTGGGCTCACCAGCAGATTCCCTTTCAAACCTCTCTTATCCCAATCCTCCGCCACCACCTCCTCCGCCGCCGCCTCCTCTTCCTCCTCCGCCGCCGCCGCTCCTCCCTTCCGAACCTCAATTTCCCCTCTCCCTCTCTCCTCCTCTGAATCCAATGCCTTCGGATTCCGCCTTCACAACTCCAATTCTCTGCGTCTTATCGTCTCCTCTCCAATTCGTCGCTCTGTATTTGCTCGATTCGGCGGTGCTCCTCGATCTTCAGGACCTGCGGATTCTAAGCGCTCCGACGATGATTTTGGTCTCGATGTTTCCGCCATCAGGTCAAATAATGTGAGACTTATTGATGCACAACAGAACATGGTTGGAATCGTGTCAAAGGCCCAGGCCATTCAAATGGCTGAAGACGCTGAACTTGATTTGGTTGTATTATCACCCGATGCAGATCCTCCAGTTGTCAGAATAATGGATTACAATAAATATAGGTATGAATTGCAAAAGAAGAAAAGAGGACAGCAGAAGAAAAGTGCTGCAACTCGCATGGATTTGAAAGAGCTTAAAATGGGTTATAACATTGACCAACATGATTACTCTGTTCGTTTGAAAGCTGCAAGGAGGTTTTTGAAGGATGGGGACAAGGTTAAAGTTATAGTGAACTTGAAAGGTCGTGAGAATGAGTTCAGAAATATAGCAATTGAGCTCATCAGACGTTTCCAGGATGAGATTGGGGAGCTTGCAACTGAAGAGGCCAAGAACTTCAGAGACAGGAATATATTTATTGTTCTAGTTCCTAACAAGGCTGTTCTACAGAAGGCTCAAGAACCCCCCAAGAAAAAGGACAATCCCGCCATAAATGAAGTTTCGGCTGGTGTTTAA

Protein sequence

MAGLTSRFPFKPLLSQSSATTSSAAASSSSSAAAAPPFRTSISPLPLSSSESNAFGFRLHNSNSLRLIVSSPIRRSVFARFGGAPRSSGPADSKRSDDDFGLDVSAIRSNNVRLIDAQQNMVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSAATRMDLKELKMGYNIDQHDYSVRLKAARRFLKDGDKVKVIVNLKGRENEFRNIAIELIRRFQDEIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDNPAINEVSAGV

Homology

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BLAST of MC08g2148 vs. ExPASy Swiss-Prot
Match: Q94B52 (Translation initiation factor IF3-4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IF3-4 PE=2 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 3.2e-76
Identity = 172/294 (58.50%), Postives = 204/294 (69.39%), Query Frame = 0


Query: 1   MAGLTSRFPFKPLLSQSSATTSSAAASSSSSAAAAPPFRTSISPLPLSSSESNAFGFRLH 60
           MAG+TS   F  +L+ ++ T S    S         P  + +S  P       A   R  
Sbjct: 1   MAGITSTVGFNAILAGATKTVSHPVKSKLFGLRLCVPEFSIVSLSPYHHRRCPAITCRYG 60

Query: 61  NSNSLRLIVSSPIRRSVFARFGGAPRSSGPAD----SKRSDDDFGLDVSAIRSNNVRLID 120
                                GG   S  P D     K S+DD  LD+SAIRS  VRLID
Sbjct: 61  G--------------------GGGGGSRFPGDRRGRQKESEDDDSLDISAIRSATVRLID 120

Query: 121 AQQNMVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQQKKS 180
            QQNM+G+VSK +A++ AEDAELDLV+LSPDADPPVVR+MDY+KYRYE QK+K+ QQKK+
Sbjct: 121 DQQNMIGLVSKEEAVRRAEDAELDLVILSPDADPPVVRMMDYSKYRYEQQKRKKEQQKKT 180

Query: 181 AATRMDLKELKMGYNIDQHDYSVRLKAARRFLKDGDKVKVIVNLKGRENEFRNIAIELIR 240
             TRMDLKELKMGYNIDQHDYSVR++AAR+FL+DGDKVKVIVN+KGRENEFRNIAIEL+R
Sbjct: 181 --TRMDLKELKMGYNIDQHDYSVRMRAARKFLQDGDKVKVIVNMKGRENEFRNIAIELLR 240

Query: 241 RFQDEIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQE-PPKKKDNPAINEVSA 290
           RFQ EIGEL TEE+KNFRDRN+FIVLVPNK V++K QE PPKKK  PA ++VSA
Sbjct: 241 RFQTEIGELGTEESKNFRDRNLFIVLVPNKEVIRKVQEPPPKKKKKPADDKVSA 272

[Close]

BLAST of MC08g2148 vs. ExPASy Swiss-Prot
Match: O82234 (Translation initiation factor IF3-2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IF3-2 PE=2 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 1.2e-75
Identity = 163/258 (63.18%), Postives = 200/258 (77.52%), Query Frame = 0


Query: 41  SISPLPLSSSESNAFGFRLHNSN-SLRLIVSSPIRR-SVFARFGGAP---RSSGP---AD 100
           ++S   L S +S     RL  +  S   +  SP R  +V  RFGG     R SG      
Sbjct: 21  TVSSHSLFSVDSKLCSLRLSKTELSFTNLTPSPRRAFAVTCRFGGGGGGYRFSGDNRRGR 80

Query: 101 SKRSDDDFGLDVSAIRSNNVRLIDAQQNMVGIVSKAQAIQMAEDAELDLVVLSPDADPPV 160
            K ++ D  LD+S+IRS  VRLID QQNM+G+VSK +A++MA+DAELDLV+LSPDADPPV
Sbjct: 81  PKEAEIDEALDISSIRSATVRLIDGQQNMLGLVSKDEAVRMADDAELDLVILSPDADPPV 140

Query: 161 VRIMDYNKYRYELQKKKRGQQKKSAATRMDLKELKMGYNIDQHDYSVRLKAARRFLKDGD 220
           V++MDY+KYRYE QK+K+ QQKK+  TRMDLKELKMGYNIDQHDYSVRL+AA++FL+DGD
Sbjct: 141 VKMMDYSKYRYEQQKRKKDQQKKT--TRMDLKELKMGYNIDQHDYSVRLRAAQKFLQDGD 200

Query: 221 KVKVIVNLKGRENEFRNIAIELIRRFQDEIGELATEEAKNFRDRNIFIVLVPNKAVLQKA 280
           KVKVIV++KGRENEFRNIAIEL+RRFQ EIGELATEE+KNFRDRN+FI+LVPNK +++K 
Sbjct: 201 KVKVIVSMKGRENEFRNIAIELLRRFQTEIGELATEESKNFRDRNMFIILVPNKEMIRKP 260

Query: 281 QEPP-KKKDNPAINEVSA 290
           QEPP +KK   A NE SA
Sbjct: 261 QEPPTRKKKKTAENEASA 276

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BLAST of MC08g2148 vs. ExPASy Swiss-Prot
Match: B0JKQ1 (Translation initiation factor IF-3 OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=infC PE=3 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.1e-31
Identity = 75/176 (42.61%), Postives = 115/176 (65.34%), Query Frame = 0


Query: 92  DSKRSDDDFGLDVSAIRSNNVRLIDAQQNMVGIVSKAQAIQMAEDAELDLVVLSPDADPP 151
           D +R+  D       IR   +R+ID+  + +GI++ A+A+++AE+ ELDLV++S  A PP
Sbjct: 3   DKRRTTRDLPQINERIRFPEIRVIDSDGSQLGIITPAEALRVAEEKELDLVLVSETASPP 62

Query: 152 VVRIMDYNKYRYELQKKKRGQQKKSAATRMDLKELKMGYNIDQHDYSVRLKAARRFLKDG 211
           V RIMDY KY++E +KK R  +KK      D+KE+KM Y ID+HDY+VR+  A+RFLK G
Sbjct: 63  VCRIMDYGKYKFEQEKKAREAKKKQHTA--DIKEVKMRYKIDEHDYNVRVNQAQRFLKAG 122

Query: 212 DKVKVIVNLKGRENEFRNIAIELIRRFQDEIGELA-TEEAKNFRDRNIFIVLVPNK 267
           DKVK  +  +GRE +  N+A EL+ R   ++ ++A  ++A     RN+ ++L P K
Sbjct: 123 DKVKATITFRGREIQHSNLAEELLARMAKDLQDVAEVQQAPKKEGRNMMMMLSPKK 176

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BLAST of MC08g2148 vs. ExPASy Swiss-Prot
Match: Q8YNE3 (Translation initiation factor IF-3 OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=infC PE=3 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 7.0e-31
Identity = 75/178 (42.13%), Postives = 112/178 (62.92%), Query Frame = 0


Query: 90  PADSKRSDDDFGLDVSAIRSNNVRLIDAQQNMVGIVSKAQAIQMAEDAELDLVVLSPDAD 149
           P   K+   D       IR   +R+ID   + +GI++  +A+Q+AE+ ELDLV+LS  AD
Sbjct: 2   PVIEKKRTRDLPQINERIRFPKIRVIDTDGSQLGILTPQEALQLAEEKELDLVLLSDKAD 61

Query: 150 PPVVRIMDYNKYRYELQKKKRGQQKKSAATRMDLKELKMGYNIDQHDYSVRLKAARRFLK 209
           PPV RIMDY KY++E +KK R  +KK      D+KE+KM Y I++HDY+VR+K A RFLK
Sbjct: 62  PPVCRIMDYGKYKFEQEKKAREARKKQHTA--DVKEVKMRYKIEEHDYNVRVKQAERFLK 121

Query: 210 DGDKVKVIVNLKGRENEFRNIAIELIRRFQDEIGELA-TEEAKNFRDRNIFIVLVPNK 267
           DGDKVK  V  +GRE +  ++A +L++R   ++      ++A     RN+ +++ P K
Sbjct: 122 DGDKVKATVMFRGREIQHSDLAEDLLKRMATDLEPFGEVQQAPKKEGRNMMMLISPKK 177

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BLAST of MC08g2148 vs. ExPASy Swiss-Prot
Match: Q3MBH6 (Translation initiation factor IF-3 OS=Trichormus variabilis (strain ATCC 29413 / PCC 7937) OX=240292 GN=infC PE=3 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 7.0e-31
Identity = 75/178 (42.13%), Postives = 112/178 (62.92%), Query Frame = 0


Query: 90  PADSKRSDDDFGLDVSAIRSNNVRLIDAQQNMVGIVSKAQAIQMAEDAELDLVVLSPDAD 149
           P   K+   D       IR   +R+ID   + +GI++  +A+Q+AE+ ELDLV+LS  AD
Sbjct: 2   PVIEKKRTRDLPQINERIRFPKIRVIDTDGSQLGILTPQEALQLAEEKELDLVLLSDKAD 61

Query: 150 PPVVRIMDYNKYRYELQKKKRGQQKKSAATRMDLKELKMGYNIDQHDYSVRLKAARRFLK 209
           PPV RIMDY KY++E +KK R  +KK      D+KE+KM Y I++HDY+VR+K A RFLK
Sbjct: 62  PPVCRIMDYGKYKFEQEKKAREARKKQHTA--DVKEVKMRYKIEEHDYNVRVKQAERFLK 121

Query: 210 DGDKVKVIVNLKGRENEFRNIAIELIRRFQDEIGELA-TEEAKNFRDRNIFIVLVPNK 267
           DGDKVK  V  +GRE +  ++A +L++R   ++      ++A     RN+ +++ P K
Sbjct: 122 DGDKVKATVMFRGREIQHSDLAEDLLKRMATDLEPFGEVQQAPKKEGRNMMMLISPKK 177

[Close]

BLAST of MC08g2148 vs. NCBI nr
Match: XP_022158178.1 (translation initiation factor IF3-4, chloroplastic [Momordica charantia] >XP_022158179.1 translation initiation factor IF3-4, chloroplastic [Momordica charantia])

HSP 1 Score: 542 bits (1396), Expect = 1.12e-193
Identity = 291/291 (100.00%), Postives = 291/291 (100.00%), Query Frame = 0


Query: 1   MAGLTSRFPFKPLLSQSSATTSSAAASSSSSAAAAPPFRTSISPLPLSSSESNAFGFRLH 60
           MAGLTSRFPFKPLLSQSSATTSSAAASSSSSAAAAPPFRTSISPLPLSSSESNAFGFRLH
Sbjct: 1   MAGLTSRFPFKPLLSQSSATTSSAAASSSSSAAAAPPFRTSISPLPLSSSESNAFGFRLH 60

Query: 61  NSNSLRLIVSSPIRRSVFARFGGAPRSSGPADSKRSDDDFGLDVSAIRSNNVRLIDAQQN 120
           NSNSLRLIVSSPIRRSVFARFGGAPRSSGPADSKRSDDDFGLDVSAIRSNNVRLIDAQQN
Sbjct: 61  NSNSLRLIVSSPIRRSVFARFGGAPRSSGPADSKRSDDDFGLDVSAIRSNNVRLIDAQQN 120

Query: 121 MVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSAATR 180
           MVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSAATR
Sbjct: 121 MVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSAATR 180

Query: 181 MDLKELKMGYNIDQHDYSVRLKAARRFLKDGDKVKVIVNLKGRENEFRNIAIELIRRFQD 240
           MDLKELKMGYNIDQHDYSVRLKAARRFLKDGDKVKVIVNLKGRENEFRNIAIELIRRFQD
Sbjct: 181 MDLKELKMGYNIDQHDYSVRLKAARRFLKDGDKVKVIVNLKGRENEFRNIAIELIRRFQD 240

Query: 241 EIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDNPAINEVSAGV 291
           EIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDNPAINEVSAGV
Sbjct: 241 EIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDNPAINEVSAGV 291

[Close]

BLAST of MC08g2148 vs. NCBI nr
Match: XP_038891171.1 (translation initiation factor IF3-4, chloroplastic [Benincasa hispida])

HSP 1 Score: 480 bits (1236), Expect = 3.11e-169
Identity = 264/297 (88.89%), Postives = 281/297 (94.61%), Query Frame = 0


Query: 1   MAGLTSRFPFKPLLSQSS-ATTSSAAASSSSSAAAAPPFRTSISPLPLSSSESNAFGFRL 60
           MAGL+ RFPFKPLLSQS+ ATT+SAAA++SSS+A   PFRTSI PLPLSSS++NAF FRL
Sbjct: 1   MAGLSGRFPFKPLLSQSTTATTTSAAAATSSSSAT--PFRTSIRPLPLSSSKANAFKFRL 60

Query: 61  HNSNSLRLIVS----SPIRRSVFARFGGAPRSSGPADSKRSDDDFGLDVSAIRSNNVRLI 120
             SNSLRL+VS    SPIR SVF+R+GGAPRSSGPADSKRSDD+FGLDVSAIRSNNVRLI
Sbjct: 61  RKSNSLRLVVSFPVSSPIRTSVFSRYGGAPRSSGPADSKRSDDEFGLDVSAIRSNNVRLI 120

Query: 121 DAQQNMVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQQKK 180
           DAQQNMVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQQKK
Sbjct: 121 DAQQNMVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQQKK 180

Query: 181 SAATRMDLKELKMGYNIDQHDYSVRLKAARRFLKDGDKVKVIVNLKGRENEFRNIAIELI 240
           SAATRMDLKELKMGYNIDQHDYSVRL+AA++FLKDGDKVKVIVNLKGRENEFRNIAIELI
Sbjct: 181 SAATRMDLKELKMGYNIDQHDYSVRLRAAQKFLKDGDKVKVIVNLKGRENEFRNIAIELI 240

Query: 241 RRFQDEIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQE-PPKKKDNPAINEVSAGV 291
           RRFQ+EIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQE PPKKKD PA+NEVSAGV
Sbjct: 241 RRFQEEIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEQPPKKKDKPAVNEVSAGV 295

[Close]

BLAST of MC08g2148 vs. NCBI nr
Match: XP_008452687.1 (PREDICTED: translation initiation factor IF-3 [Cucumis melo])

HSP 1 Score: 465 bits (1196), Expect = 4.13e-163
Identity = 258/299 (86.29%), Postives = 271/299 (90.64%), Query Frame = 0


Query: 1   MAGLTSRFPFKPLLSQSSA---TTSSAAASSSSSAAAAPPFRTSISPLPLSSS-ESNAFG 60
           MAGL+  FPFK LLSQSSA   TTS AAA +SSS+    PFRTS   LP+SSS  SNAF 
Sbjct: 1   MAGLSGSFPFKSLLSQSSAASTTTSPAAAPTSSSSTT--PFRTSTPSLPISSSFNSNAFK 60

Query: 61  FRLHNSNSLRLI----VSSPIRRSVFARFGGAPRSSGPADSKRSDDDFGLDVSAIRSNNV 120
            RLH SNSLRL+    VSSPIR SVFAR GGAPRSSGP DSKRS+D+FGLDVSAIRSNNV
Sbjct: 61  LRLHKSNSLRLVFSFPVSSPIRTSVFARLGGAPRSSGPPDSKRSNDEFGLDVSAIRSNNV 120

Query: 121 RLIDAQQNMVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQ 180
           RLIDAQQNMVG+VSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQ
Sbjct: 121 RLIDAQQNMVGVVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQ 180

Query: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAARRFLKDGDKVKVIVNLKGRENEFRNIAI 240
           QKKSAATRMDLKELKMGYNIDQHDYSVRLKAA++FLKDGDKVKVIVNLKGRENEFRNIAI
Sbjct: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI 240

Query: 241 ELIRRFQDEIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDNPAINEVSAGV 291
           ELIRRFQ+E+GELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKD PA+NEVSAGV
Sbjct: 241 ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNEVSAGV 297

[Close]

BLAST of MC08g2148 vs. NCBI nr
Match: XP_004144726.1 (translation initiation factor IF3-4, chloroplastic [Cucumis sativus] >KGN61058.1 hypothetical protein Csa_021304 [Cucumis sativus])

HSP 1 Score: 457 bits (1175), Expect = 6.99e-160
Identity = 248/299 (82.94%), Postives = 269/299 (89.97%), Query Frame = 0


Query: 1   MAGLTSRFPFKPLLSQSSA----TTSSAAASSSSSAAAAPPFRTSISPLPLSSSESNAFG 60
           MAGL+  FPFK LLSQSSA    T+SS+AA   +S+++  PFRTS   LP+SSS SNAF 
Sbjct: 1   MAGLSGSFPFKSLLSQSSAAATTTSSSSAAPPPTSSSSTTPFRTSFPSLPISSSNSNAFK 60

Query: 61  FRLHNSNSLRLI----VSSPIRRSVFARFGGAPRSSGPADSKRSDDDFGLDVSAIRSNNV 120
           FRLH SNSLRL+    VSSPIR SVFAR GGAPRSSGP DSKRS+D+ GLD+SAI+S NV
Sbjct: 61  FRLHKSNSLRLLFSSPVSSPIRTSVFARLGGAPRSSGPPDSKRSNDEIGLDISAIKSINV 120

Query: 121 RLIDAQQNMVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQ 180
           RLIDA+QNMVG+VSKAQAIQMAEDAELDLVV+SPDADPPVVRIMDYNKYRYELQKKKRGQ
Sbjct: 121 RLIDAEQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQ 180

Query: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAARRFLKDGDKVKVIVNLKGRENEFRNIAI 240
           QKKSAATRMDLKELKMGYNIDQHDYSVRLKAA++FLKDGDKVKVIVNLKGRENEFRNIAI
Sbjct: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI 240

Query: 241 ELIRRFQDEIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDNPAINEVSAGV 291
           ELIRRFQ+E+GELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKD PA+N VSA V
Sbjct: 241 ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNGVSASV 299

[Close]

BLAST of MC08g2148 vs. NCBI nr
Match: XP_022969585.1 (translation initiation factor IF3-4, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 446 bits (1148), Expect = 7.01e-156
Identity = 242/292 (82.88%), Postives = 261/292 (89.38%), Query Frame = 0


Query: 1   MAGLTSRFPFKPLLSQSSAT-TSSAAASSSSSAAAAPPFRTSISPLPLSSSESNAFGFRL 60
           MAGLT RFPFKPLLS SSAT T+SAAA SSS+ A A P RTSISPLPLSSS+ NAF FR 
Sbjct: 1   MAGLTGRFPFKPLLSHSSATPTTSAAAPSSSATAIATPIRTSISPLPLSSSKFNAFRFRH 60

Query: 61  HNSNSLRLIVSSPIRRSVFARFGGAPRSSGPADSKRSDDDFGLDVSAIRSNNVRLIDAQQ 120
           HNSNSL L+ ++  R +V +++GGA RSSGPADSKR DD  G+D+SAIRSN VRLIDAQQ
Sbjct: 61  HNSNSLHLVFTTSTRSTVVSQYGGASRSSGPADSKRFDDGLGVDISAIRSNTVRLIDAQQ 120

Query: 121 NMVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSAAT 180
           NMVGIVSK QAIQMA+DAELDLVVLSPDADPPVVRIMDY+KYRYE QKKKRGQQKKSAAT
Sbjct: 121 NMVGIVSKVQAIQMADDAELDLVVLSPDADPPVVRIMDYSKYRYEQQKKKRGQQKKSAAT 180

Query: 181 RMDLKELKMGYNIDQHDYSVRLKAARRFLKDGDKVKVIVNLKGRENEFRNIAIELIRRFQ 240
           RMDLKELKMGYNIDQHDYSVRLKAAR+FLKDGDKVKVIVNLKGREN F+NIAIELIRRFQ
Sbjct: 181 RMDLKELKMGYNIDQHDYSVRLKAARKFLKDGDKVKVIVNLKGRENAFKNIAIELIRRFQ 240

Query: 241 DEIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDNPAINEVSAGV 291
           +EIGELATEEAKN RDRN+FIVL PNKAVLQKAQEPPK KDN  +NEVSA V
Sbjct: 241 EEIGELATEEAKNLRDRNMFIVLAPNKAVLQKAQEPPKVKDNLVVNEVSASV 292

[Close]

BLAST of MC08g2148 vs. ExPASy TrEMBL
Match: A0A6J1DYM5 (Translation initiation factor IF-3 OS=Momordica charantia OX=3673 GN=LOC111024717 PE=3 SV=1)

HSP 1 Score: 542 bits (1396), Expect = 5.44e-194
Identity = 291/291 (100.00%), Postives = 291/291 (100.00%), Query Frame = 0


Query: 1   MAGLTSRFPFKPLLSQSSATTSSAAASSSSSAAAAPPFRTSISPLPLSSSESNAFGFRLH 60
           MAGLTSRFPFKPLLSQSSATTSSAAASSSSSAAAAPPFRTSISPLPLSSSESNAFGFRLH
Sbjct: 1   MAGLTSRFPFKPLLSQSSATTSSAAASSSSSAAAAPPFRTSISPLPLSSSESNAFGFRLH 60

Query: 61  NSNSLRLIVSSPIRRSVFARFGGAPRSSGPADSKRSDDDFGLDVSAIRSNNVRLIDAQQN 120
           NSNSLRLIVSSPIRRSVFARFGGAPRSSGPADSKRSDDDFGLDVSAIRSNNVRLIDAQQN
Sbjct: 61  NSNSLRLIVSSPIRRSVFARFGGAPRSSGPADSKRSDDDFGLDVSAIRSNNVRLIDAQQN 120

Query: 121 MVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSAATR 180
           MVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSAATR
Sbjct: 121 MVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSAATR 180

Query: 181 MDLKELKMGYNIDQHDYSVRLKAARRFLKDGDKVKVIVNLKGRENEFRNIAIELIRRFQD 240
           MDLKELKMGYNIDQHDYSVRLKAARRFLKDGDKVKVIVNLKGRENEFRNIAIELIRRFQD
Sbjct: 181 MDLKELKMGYNIDQHDYSVRLKAARRFLKDGDKVKVIVNLKGRENEFRNIAIELIRRFQD 240

Query: 241 EIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDNPAINEVSAGV 291
           EIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDNPAINEVSAGV
Sbjct: 241 EIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDNPAINEVSAGV 291

[Close]

BLAST of MC08g2148 vs. ExPASy TrEMBL
Match: A0A1S3BV82 (Translation initiation factor IF-3 OS=Cucumis melo OX=3656 GN=LOC103493630 PE=3 SV=1)

HSP 1 Score: 465 bits (1196), Expect = 2.00e-163
Identity = 258/299 (86.29%), Postives = 271/299 (90.64%), Query Frame = 0


Query: 1   MAGLTSRFPFKPLLSQSSA---TTSSAAASSSSSAAAAPPFRTSISPLPLSSS-ESNAFG 60
           MAGL+  FPFK LLSQSSA   TTS AAA +SSS+    PFRTS   LP+SSS  SNAF 
Sbjct: 1   MAGLSGSFPFKSLLSQSSAASTTTSPAAAPTSSSSTT--PFRTSTPSLPISSSFNSNAFK 60

Query: 61  FRLHNSNSLRLI----VSSPIRRSVFARFGGAPRSSGPADSKRSDDDFGLDVSAIRSNNV 120
            RLH SNSLRL+    VSSPIR SVFAR GGAPRSSGP DSKRS+D+FGLDVSAIRSNNV
Sbjct: 61  LRLHKSNSLRLVFSFPVSSPIRTSVFARLGGAPRSSGPPDSKRSNDEFGLDVSAIRSNNV 120

Query: 121 RLIDAQQNMVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQ 180
           RLIDAQQNMVG+VSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQ
Sbjct: 121 RLIDAQQNMVGVVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQ 180

Query: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAARRFLKDGDKVKVIVNLKGRENEFRNIAI 240
           QKKSAATRMDLKELKMGYNIDQHDYSVRLKAA++FLKDGDKVKVIVNLKGRENEFRNIAI
Sbjct: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI 240

Query: 241 ELIRRFQDEIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDNPAINEVSAGV 291
           ELIRRFQ+E+GELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKD PA+NEVSAGV
Sbjct: 241 ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNEVSAGV 297

[Close]

BLAST of MC08g2148 vs. ExPASy TrEMBL
Match: A0A0A0LH34 (Translation initiation factor IF-3 OS=Cucumis sativus OX=3659 GN=Csa_2G036070 PE=3 SV=1)

HSP 1 Score: 457 bits (1175), Expect = 3.38e-160
Identity = 248/299 (82.94%), Postives = 269/299 (89.97%), Query Frame = 0


Query: 1   MAGLTSRFPFKPLLSQSSA----TTSSAAASSSSSAAAAPPFRTSISPLPLSSSESNAFG 60
           MAGL+  FPFK LLSQSSA    T+SS+AA   +S+++  PFRTS   LP+SSS SNAF 
Sbjct: 1   MAGLSGSFPFKSLLSQSSAAATTTSSSSAAPPPTSSSSTTPFRTSFPSLPISSSNSNAFK 60

Query: 61  FRLHNSNSLRLI----VSSPIRRSVFARFGGAPRSSGPADSKRSDDDFGLDVSAIRSNNV 120
           FRLH SNSLRL+    VSSPIR SVFAR GGAPRSSGP DSKRS+D+ GLD+SAI+S NV
Sbjct: 61  FRLHKSNSLRLLFSSPVSSPIRTSVFARLGGAPRSSGPPDSKRSNDEIGLDISAIKSINV 120

Query: 121 RLIDAQQNMVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQ 180
           RLIDA+QNMVG+VSKAQAIQMAEDAELDLVV+SPDADPPVVRIMDYNKYRYELQKKKRGQ
Sbjct: 121 RLIDAEQNMVGVVSKAQAIQMAEDAELDLVVVSPDADPPVVRIMDYNKYRYELQKKKRGQ 180

Query: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAARRFLKDGDKVKVIVNLKGRENEFRNIAI 240
           QKKSAATRMDLKELKMGYNIDQHDYSVRLKAA++FLKDGDKVKVIVNLKGRENEFRNIAI
Sbjct: 181 QKKSAATRMDLKELKMGYNIDQHDYSVRLKAAKKFLKDGDKVKVIVNLKGRENEFRNIAI 240

Query: 241 ELIRRFQDEIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDNPAINEVSAGV 291
           ELIRRFQ+E+GELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKD PA+N VSA V
Sbjct: 241 ELIRRFQEEVGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDKPAVNGVSASV 299

[Close]

BLAST of MC08g2148 vs. ExPASy TrEMBL
Match: A0A6J1I0C0 (Translation initiation factor IF-3 OS=Cucurbita maxima OX=3661 GN=LOC111468564 PE=3 SV=1)

HSP 1 Score: 446 bits (1148), Expect = 3.40e-156
Identity = 242/292 (82.88%), Postives = 261/292 (89.38%), Query Frame = 0


Query: 1   MAGLTSRFPFKPLLSQSSAT-TSSAAASSSSSAAAAPPFRTSISPLPLSSSESNAFGFRL 60
           MAGLT RFPFKPLLS SSAT T+SAAA SSS+ A A P RTSISPLPLSSS+ NAF FR 
Sbjct: 1   MAGLTGRFPFKPLLSHSSATPTTSAAAPSSSATAIATPIRTSISPLPLSSSKFNAFRFRH 60

Query: 61  HNSNSLRLIVSSPIRRSVFARFGGAPRSSGPADSKRSDDDFGLDVSAIRSNNVRLIDAQQ 120
           HNSNSL L+ ++  R +V +++GGA RSSGPADSKR DD  G+D+SAIRSN VRLIDAQQ
Sbjct: 61  HNSNSLHLVFTTSTRSTVVSQYGGASRSSGPADSKRFDDGLGVDISAIRSNTVRLIDAQQ 120

Query: 121 NMVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSAAT 180
           NMVGIVSK QAIQMA+DAELDLVVLSPDADPPVVRIMDY+KYRYE QKKKRGQQKKSAAT
Sbjct: 121 NMVGIVSKVQAIQMADDAELDLVVLSPDADPPVVRIMDYSKYRYEQQKKKRGQQKKSAAT 180

Query: 181 RMDLKELKMGYNIDQHDYSVRLKAARRFLKDGDKVKVIVNLKGRENEFRNIAIELIRRFQ 240
           RMDLKELKMGYNIDQHDYSVRLKAAR+FLKDGDKVKVIVNLKGREN F+NIAIELIRRFQ
Sbjct: 181 RMDLKELKMGYNIDQHDYSVRLKAARKFLKDGDKVKVIVNLKGRENAFKNIAIELIRRFQ 240

Query: 241 DEIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDNPAINEVSAGV 291
           +EIGELATEEAKN RDRN+FIVL PNKAVLQKAQEPPK KDN  +NEVSA V
Sbjct: 241 EEIGELATEEAKNLRDRNMFIVLAPNKAVLQKAQEPPKVKDNLVVNEVSASV 292

[Close]

BLAST of MC08g2148 vs. ExPASy TrEMBL
Match: A0A6J1G3Y5 (Translation initiation factor IF-3 OS=Cucurbita moschata OX=3662 GN=LOC111450575 PE=3 SV=1)

HSP 1 Score: 442 bits (1138), Expect = 1.51e-154
Identity = 240/292 (82.19%), Postives = 264/292 (90.41%), Query Frame = 0


Query: 1   MAGLTSRFPFKPLLSQSSATTSSAAASSSSSAAAAPPFRTSISPLPLSSSESNAFGFRLH 60
           MAGL+ RFPFK + SQSSAT ++   +SSSS+A   PFRTSI PLPL SS+SNAF FRLH
Sbjct: 1   MAGLSGRFPFKSISSQSSATATATTTTSSSSSAT--PFRTSIFPLPLPSSKSNAFRFRLH 60

Query: 61  NSNSLRLIVSSPIRRSVFARFGGAPRSSGPADSKRSDDDFGLDVSAIRSNNVRLIDAQQN 120
           NSNSLRL VSSPIR SVFAR+GGAPRSSGPA SKRS+D   LDVS+IRSNNVRLID QQN
Sbjct: 61  NSNSLRL-VSSPIRTSVFARYGGAPRSSGPAGSKRSEDSLALDVSSIRSNNVRLIDEQQN 120

Query: 121 MVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQQKKSAATR 180
           MVGIV K+QAIQMAEDAELDLVVLSPDADPPVVRIMDY+KYRY+LQKKKR QQKKSAATR
Sbjct: 121 MVGIVPKSQAIQMAEDAELDLVVLSPDADPPVVRIMDYSKYRYDLQKKKREQQKKSAATR 180

Query: 181 MDLKELKMGYNIDQHDYSVRLKAARRFLKDGDKVKVIVNLKGRENEFRNIAIELIRRFQD 240
           MDLKELKMGY IDQHDYSVRL+AA++FL+DGDKVKVIVNLKGRENEFR IAIEL++RFQ+
Sbjct: 181 MDLKELKMGYKIDQHDYSVRLRAAKKFLQDGDKVKVIVNLKGRENEFRKIAIELVKRFQE 240

Query: 241 EIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKKKDNPA-INEVSAGV 291
           E+GELATEEAKNFRDRN+FIVL PNKAVLQK+QEPPKKKD PA +NEVSA V
Sbjct: 241 ELGELATEEAKNFRDRNVFIVLAPNKAVLQKSQEPPKKKDKPAAVNEVSARV 289

[Close]

BLAST of MC08g2148 vs. TAIR 10
Match: AT4G30690.1 (Translation initiation factor 3 protein )

HSP 1 Score: 286.6 bits (732), Expect = 2.3e-77
Identity = 172/294 (58.50%), Postives = 204/294 (69.39%), Query Frame = 0


Query: 1   MAGLTSRFPFKPLLSQSSATTSSAAASSSSSAAAAPPFRTSISPLPLSSSESNAFGFRLH 60
           MAG+TS   F  +L+ ++ T S    S         P  + +S  P       A   R  
Sbjct: 1   MAGITSTVGFNAILAGATKTVSHPVKSKLFGLRLCVPEFSIVSLSPYHHRRCPAITCRYG 60

Query: 61  NSNSLRLIVSSPIRRSVFARFGGAPRSSGPAD----SKRSDDDFGLDVSAIRSNNVRLID 120
                                GG   S  P D     K S+DD  LD+SAIRS  VRLID
Sbjct: 61  G--------------------GGGGGSRFPGDRRGRQKESEDDDSLDISAIRSATVRLID 120

Query: 121 AQQNMVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQQKKS 180
            QQNM+G+VSK +A++ AEDAELDLV+LSPDADPPVVR+MDY+KYRYE QK+K+ QQKK+
Sbjct: 121 DQQNMIGLVSKEEAVRRAEDAELDLVILSPDADPPVVRMMDYSKYRYEQQKRKKEQQKKT 180

Query: 181 AATRMDLKELKMGYNIDQHDYSVRLKAARRFLKDGDKVKVIVNLKGRENEFRNIAIELIR 240
             TRMDLKELKMGYNIDQHDYSVR++AAR+FL+DGDKVKVIVN+KGRENEFRNIAIEL+R
Sbjct: 181 --TRMDLKELKMGYNIDQHDYSVRMRAARKFLQDGDKVKVIVNMKGRENEFRNIAIELLR 240

Query: 241 RFQDEIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQE-PPKKKDNPAINEVSA 290
           RFQ EIGEL TEE+KNFRDRN+FIVLVPNK V++K QE PPKKK  PA ++VSA
Sbjct: 241 RFQTEIGELGTEESKNFRDRNLFIVLVPNKEVIRKVQEPPPKKKKKPADDKVSA 272

[Close]

BLAST of MC08g2148 vs. TAIR 10
Match: AT2G24060.1 (Translation initiation factor 3 protein )

HSP 1 Score: 284.6 bits (727), Expect = 8.7e-77
Identity = 163/258 (63.18%), Postives = 200/258 (77.52%), Query Frame = 0


Query: 41  SISPLPLSSSESNAFGFRLHNSN-SLRLIVSSPIRR-SVFARFGGAP---RSSGP---AD 100
           ++S   L S +S     RL  +  S   +  SP R  +V  RFGG     R SG      
Sbjct: 21  TVSSHSLFSVDSKLCSLRLSKTELSFTNLTPSPRRAFAVTCRFGGGGGGYRFSGDNRRGR 80

Query: 101 SKRSDDDFGLDVSAIRSNNVRLIDAQQNMVGIVSKAQAIQMAEDAELDLVVLSPDADPPV 160
            K ++ D  LD+S+IRS  VRLID QQNM+G+VSK +A++MA+DAELDLV+LSPDADPPV
Sbjct: 81  PKEAEIDEALDISSIRSATVRLIDGQQNMLGLVSKDEAVRMADDAELDLVILSPDADPPV 140

Query: 161 VRIMDYNKYRYELQKKKRGQQKKSAATRMDLKELKMGYNIDQHDYSVRLKAARRFLKDGD 220
           V++MDY+KYRYE QK+K+ QQKK+  TRMDLKELKMGYNIDQHDYSVRL+AA++FL+DGD
Sbjct: 141 VKMMDYSKYRYEQQKRKKDQQKKT--TRMDLKELKMGYNIDQHDYSVRLRAAQKFLQDGD 200

Query: 221 KVKVIVNLKGRENEFRNIAIELIRRFQDEIGELATEEAKNFRDRNIFIVLVPNKAVLQKA 280
           KVKVIV++KGRENEFRNIAIEL+RRFQ EIGELATEE+KNFRDRN+FI+LVPNK +++K 
Sbjct: 201 KVKVIVSMKGRENEFRNIAIELLRRFQTEIGELATEESKNFRDRNMFIILVPNKEMIRKP 260

Query: 281 QEPP-KKKDNPAINEVSA 290
           QEPP +KK   A NE SA
Sbjct: 261 QEPPTRKKKKTAENEASA 276

[Close]

BLAST of MC08g2148 vs. TAIR 10
Match: AT4G30690.2 (Translation initiation factor 3 protein )

HSP 1 Score: 233.4 bits (594), Expect = 2.3e-61
Identity = 149/294 (50.68%), Postives = 178/294 (60.54%), Query Frame = 0


Query: 1   MAGLTSRFPFKPLLSQSSATTSSAAASSSSSAAAAPPFRTSISPLPLSSSESNAFGFRLH 60
           MAG+TS   F  +L+ ++ T S    S         P  + +S  P       A   R  
Sbjct: 1   MAGITSTVGFNAILAGATKTVSHPVKSKLFGLRLCVPEFSIVSLSPYHHRRCPAITCRYG 60

Query: 61  NSNSLRLIVSSPIRRSVFARFGGAPRSSGPAD----SKRSDDDFGLDVSAIRSNNVRLID 120
                                GG   S  P D     K S+DD  LD+SAIRS  VRLID
Sbjct: 61  G--------------------GGGGGSRFPGDRRGRQKESEDDDSLDISAIRSATVRLID 120

Query: 121 AQQNMVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQKKKRGQQKKS 180
            QQNM+G+VSK +A++ AEDAELDLV+LSPDADPPVVR+MDY+                 
Sbjct: 121 DQQNMIGLVSKEEAVRRAEDAELDLVILSPDADPPVVRMMDYS----------------- 180

Query: 181 AATRMDLKELKMGYNIDQHDYSVRLKAARRFLKDGDKVKVIVNLKGRENEFRNIAIELIR 240
                        YNIDQHDYSVR++AAR+FL+DGDKVKVIVN+KGRENEFRNIAIEL+R
Sbjct: 181 -------------YNIDQHDYSVRMRAARKFLQDGDKVKVIVNMKGRENEFRNIAIELLR 240

Query: 241 RFQDEIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQE-PPKKKDNPAINEVSA 290
           RFQ EIGEL TEE+KNFRDRN+FIVLVPNK V++K QE PPKKK  PA ++VSA
Sbjct: 241 RFQTEIGELGTEESKNFRDRNLFIVLVPNKEVIRKVQEPPPKKKKKPADDKVSA 244

[Close]

BLAST of MC08g2148 vs. TAIR 10
Match: AT1G34360.1 (translation initiation factor 3 (IF-3) family protein )

HSP 1 Score: 57.0 bits (136), Expect = 2.9e-08
Identity = 54/176 (30.68%), Postives = 91/176 (51.70%), Query Frame = 0


Query: 107 IRSNNVRLIDAQQNMVGIVSKAQAIQMAEDAELDLVVLSPDADPPVVRIMDYNKYRYELQ 166
           I  + VRL+  + + V  VS  +A++ A++ + DLV +  DA PPV +I+ Y+   Y+  
Sbjct: 90  ITGDYVRLVSEEGHCV--VSLREALRRAKELQCDLVEVQRDAKPPVCKIVKYSLELYKKA 149

Query: 167 K--KKRGQQKKSAATRMDLKELKMGYNIDQHDYSVRLKAARRFLKDGDKVKVI-VNLKGR 226
           K  K+R + K++ A R D+KE++    I+  D   +   A + ++ G +VK + V  K +
Sbjct: 150 KVGKERAKAKRAEAIRPDIKEIRFTPKIEAKDLKFKSDQALKLMESGYRVKCLAVPDKDK 209

Query: 227 ENEFR-NIAIELIRRFQDEIGELATEEAKNFRDRNIFIVLVPNKAVLQKAQEPPKK 279
             E      +EL+ RF   IG+ A  E+    DR   +V+V +         PPKK
Sbjct: 210 HKELEPEKLLELLFRFTCFIGD-ALVESWPEADRKGAVVIVRHAKF-----GPPKK 257

The following BLAST results are available for this feature:

BLAST of MC08g2148 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94B52	3.2e-76	58.50	Translation initiation factor IF3-4, chloroplastic OS=Arabidopsis thaliana OX=37...	[more]
O82234	1.2e-75	63.18	Translation initiation factor IF3-2, chloroplastic OS=Arabidopsis thaliana OX=37...	[more]
B0JKQ1	1.1e-31	42.61	Translation initiation factor IF-3 OS=Microcystis aeruginosa (strain NIES-843) O...	[more]
Q8YNE3	7.0e-31	42.13	Translation initiation factor IF-3 OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / ...	[more]
Q3MBH6	7.0e-31	42.13	Translation initiation factor IF-3 OS=Trichormus variabilis (strain ATCC 29413 /...	[more]

BLAST of MC08g2148 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158178.1	1.12e-193	100.00	translation initiation factor IF3-4, chloroplastic [Momordica charantia] >XP_022...	[more]
XP_038891171.1	3.11e-169	88.89	translation initiation factor IF3-4, chloroplastic [Benincasa hispida]	[more]
XP_008452687.1	4.13e-163	86.29	PREDICTED: translation initiation factor IF-3 [Cucumis melo]	[more]
XP_004144726.1	6.99e-160	82.94	translation initiation factor IF3-4, chloroplastic [Cucumis sativus] >KGN61058.1...	[more]
XP_022969585.1	7.01e-156	82.88	translation initiation factor IF3-4, chloroplastic-like [Cucurbita maxima]	[more]

BLAST of MC08g2148 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DYM5	5.44e-194	100.00	Translation initiation factor IF-3 OS=Momordica charantia OX=3673 GN=LOC11102471...	[more]
A0A1S3BV82	2.00e-163	86.29	Translation initiation factor IF-3 OS=Cucumis melo OX=3656 GN=LOC103493630 PE=3 ...	[more]
A0A0A0LH34	3.38e-160	82.94	Translation initiation factor IF-3 OS=Cucumis sativus OX=3659 GN=Csa_2G036070 PE...	[more]
A0A6J1I0C0	3.40e-156	82.88	Translation initiation factor IF-3 OS=Cucurbita maxima OX=3661 GN=LOC111468564 P...	[more]
A0A6J1G3Y5	1.51e-154	82.19	Translation initiation factor IF-3 OS=Cucurbita moschata OX=3662 GN=LOC111450575...	[more]

BLAST of MC08g2148 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G30690.1	2.3e-77	58.50	Translation initiation factor 3 protein	[more]
AT2G24060.1	8.7e-77	63.18	Translation initiation factor 3 protein	[more]
AT4G30690.2	2.3e-61	50.68	Translation initiation factor 3 protein	[more]
AT1G34360.1	2.9e-08	30.68	translation initiation factor 3 (IF-3) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036787	Translation initiation factor 3 (IF-3), N-terminal domain superfamily	GENE3D	3.10.20.80		coord: 105..170 e-value: 2.5E-27 score: 96.7
IPR036787	Translation initiation factor 3 (IF-3), N-terminal domain superfamily	SUPERFAMILY	54364	Translation initiation factor IF3, N-terminal domain	coord: 106..174
IPR019814	Translation initiation factor 3, N-terminal	PFAM	PF05198	IF3_N	coord: 106..170 e-value: 8.1E-26 score: 90.1
IPR036788	Translation initiation factor 3 (IF-3), C-terminal domain superfamily	GENE3D	3.30.110.10		coord: 171..267 e-value: 1.8E-23 score: 84.4
IPR036788	Translation initiation factor 3 (IF-3), C-terminal domain superfamily	SUPERFAMILY	55200	Translation initiation factor IF3, C-terminal domain	coord: 181..266
IPR001288	Translation initiation factor 3	TIGRFAM	TIGR00168	TIGR00168	coord: 106..266 e-value: 1.4E-47 score: 159.4
IPR001288	Translation initiation factor 3	PANTHER	PTHR10938	TRANSLATION INITIATION FACTOR IF-3	coord: 31..281
IPR001288	Translation initiation factor 3	HAMAP	MF_00080	IF_3	coord: 93..267 score: 22.460472
IPR019815	Translation initiation factor 3, C-terminal	PFAM	PF00707	IF3_C	coord: 182..265 e-value: 7.0E-23 score: 80.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 272..291
None	No IPR available	PANTHER	PTHR10938:SF6	TRANSLATION INITIATION FACTOR IF-3	coord: 31..281
IPR019813	Translation initiation factor 3, conserved site	PROSITE	PS00938	IF3	coord: 154..167

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g2148.1	MC08g2148.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032790	ribosome disassembly
biological_process	GO:0006413	translational initiation
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0043022	ribosome binding
molecular_function	GO:0003743	translation initiation factor activity