MC08g2109 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g2109 vs. ExPASy Swiss-Prot
Match: Q9FKK7 (Xylose isomerase OS=Arabidopsis thaliana OX=3702 GN=XYLA PE=2 SV=2)

HSP 1 Score: 841.6 bits (2173), Expect = 4.2e-243
Identity = 398/478 (83.26%), Postives = 435/478 (91.00%), Query Frame = 0

Query: 1   MKSRNVLVLVLCLNVLLLGAIAESPTCPAADLGGECSHSGEWEGEFFPGIPKIKYEGPDS 60
           MK     +L+LC         A+ PTCP ADLGG+CS S +W+G+FFP IPKIKYEGP S
Sbjct: 1   MKKVEFFMLLLCFIAASSLVSADPPTCP-ADLGGKCSDSDDWQGDFFPEIPKIKYEGPSS 60

Query: 61  KKPLAFKWYNADEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSLAM 120
           K PLA++WYNA+EEILGKKMKDW RFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNS++M
Sbjct: 61  KNPLAYRWYNAEEEILGKKMKDWFRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSVSM 120

Query: 121 AKRRMRANFEFINKLGVEWWCFHDRDIAPDGQTLKETNANLDAVVALAKELQ-GTKIKPL 180
           AKRRMRANFEF+ KLGV+WWCFHDRDIAPDG TL+E+N NLD V+ LAKELQ G+KIKPL
Sbjct: 121 AKRRMRANFEFLKKLGVDWWCFHDRDIAPDGTTLEESNKNLDEVIELAKELQKGSKIKPL 180

Query: 181 WGTAQLFVHPRYMHGAATSSEVAVYAYAAAQVKKAMEVTHYLGGENYVFWGGREGYQSLL 240
           WGTAQLF+HPRYMHG ATSSEV VYAYAAAQVKKAMEVTHYLGGENYVFWGGREGYQ+LL
Sbjct: 181 WGTAQLFLHPRYMHGGATSSEVGVYAYAAAQVKKAMEVTHYLGGENYVFWGGREGYQTLL 240

Query: 241 NTDMGRELDHLARFLEAAVAHKKKIGFNGTLLIEPKPQEPTKHQYDWDAATAANFLRKYG 300
           NTDMGRELDHLARF EAAVA+KKKIGF GTLLIEPKPQEPTKHQYDWDAATAANFLRKYG
Sbjct: 241 NTDMGRELDHLARFFEAAVAYKKKIGFKGTLLIEPKPQEPTKHQYDWDAATAANFLRKYG 300

Query: 301 LIDEFKLNIECNHATLSGHSCHHELETARINGLLGNIDANSGDPQVGWDTDQFLTDVGEA 360
           LIDEFKLNIECNHATLSGH+CHHELETARINGLLGNIDAN+GD Q GWDTDQFLTDVGEA
Sbjct: 301 LIDEFKLNIECNHATLSGHTCHHELETARINGLLGNIDANTGDAQTGWDTDQFLTDVGEA 360

Query: 361 TMVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLRSVKNLIEDGS 420
           TMVM+SV+KNGG+APGGFNFDAKLRRESTDVEDLFIAHISGMDT+ARGLR+   ++E+GS
Sbjct: 361 TMVMMSVIKNGGIAPGGFNFDAKLRRESTDVEDLFIAHISGMDTMARGLRNAVKILEEGS 420

Query: 421 LMELVRKRYQSFDTEIGAQIEAGKADFEYLEKKALEWGEPKVPSAKQELAEMIFQSAL 478
           L ELVRKRY ++D+E+G QIE GKADFEYLEKKA E+GEPKV SAKQELAEMIFQSA+
Sbjct: 421 LSELVRKRYATWDSELGKQIEEGKADFEYLEKKAKEFGEPKVSSAKQELAEMIFQSAM 477

BLAST of MC08g2109 vs. ExPASy Swiss-Prot
Match: Q40082 (Xylose isomerase OS=Hordeum vulgare OX=4513 GN=XYLA PE=1 SV=1)

HSP 1 Score: 805.4 bits (2079), Expect = 3.4e-232
Identity = 387/480 (80.62%), Postives = 424/480 (88.33%), Query Frame = 0

Query: 1   MKSRNVLVLVLCLNVLLLGAIAESPTCPAADLGGECSH--SGEWEGEFFPGIPKIKYEGP 60
           MK   +LVL+L  ++ L  A+A   TCP AD+G +C+   S +WEGEFFPGI KI YEGP
Sbjct: 1   MKGGELLVLLLASSLCLSAAVAAQETCP-ADIGAKCTDAASDDWEGEFFPGIDKINYEGP 60

Query: 61  DSKKPLAFKWYNADEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSL 120
            SKKPL++KWYNA+E ILGKKMKDW RFSVAFWHTFRGTGGDPFGA TK WPWEDGTNSL
Sbjct: 61  TSKKPLSYKWYNAEEVILGKKMKDWFRFSVAFWHTFRGTGGDPFGAPTKNWPWEDGTNSL 120

Query: 121 AMAKRRMRANFEFINKLGVEWWCFHDRDIAPDGQTLKETNANLDAVVALAKELQG-TKIK 180
           AMAKRRM+A+FEF+ KLGVE WCFHDRDIAPDG+TL ETNANLD +V LAK+LQ  T IK
Sbjct: 121 AMAKRRMKAHFEFMEKLGVERWCFHDRDIAPDGKTLAETNANLDEIVELAKQLQSETNIK 180

Query: 181 PLWGTAQLFVHPRYMHGAATSSEVAVYAYAAAQVKKAMEVTHYLGGENYVFWGGREGYQS 240
           PLWGTAQLF+HPRYMHGAATS EV VYAYAAAQVKKA+EVTHYLGGENYVFWGGREGYQ+
Sbjct: 181 PLWGTAQLFMHPRYMHGAATSPEVKVYAYAAAQVKKALEVTHYLGGENYVFWGGREGYQT 240

Query: 241 LLNTDMGRELDHLARFLEAAVAHKKKIGFNGTLLIEPKPQEPTKHQYDWDAATAANFLRK 300
           LLNTDM REL+HLA FL+AAV HKKKIGFNGTLLIEPKPQEPTKHQYDWD AT  +FL+K
Sbjct: 241 LLNTDMKRELEHLANFLQAAVNHKKKIGFNGTLLIEPKPQEPTKHQYDWDVATTFSFLQK 300

Query: 301 YGLIDEFKLNIECNHATLSGHSCHHELETARINGLLGNIDANSGDPQVGWDTDQFLTDVG 360
           +GL  EFK+N+ECNHATLSGHSCHHELETARIN +LGNIDAN+GDPQVGWDTD+FLTD+ 
Sbjct: 301 FGLTGEFKINVECNHATLSGHSCHHELETARINDILGNIDANTGDPQVGWDTDEFLTDIS 360

Query: 361 EATMVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLRSVKNLIED 420
           EAT++M SVVKN GLAPGGFNF AKLRRESTDVEDLFIAHISGMDT+ARG R+V  LIED
Sbjct: 361 EATLIMSSVVKNDGLAPGGFNFYAKLRRESTDVEDLFIAHISGMDTMARGRRNVVKLIED 420

Query: 421 GSLMELVRKRYQSFDTEIGAQIEAGKADFEYLEKKALEWGEPKVPSAKQELAEMIFQSAL 478
           GSL ELVRKRYQSFDTEIGA IEAGK DFE LEKKALEWGEP VPS KQELAEM+FQSAL
Sbjct: 421 GSLDELVRKRYQSFDTEIGAMIEAGKGDFETLEKKALEWGEPTVPSGKQELAEMLFQSAL 479

BLAST of MC08g2109 vs. ExPASy Swiss-Prot
Match: B9MPG8 (Xylose isomerase OS=Caldicellulosiruptor bescii (strain ATCC BAA-1888 / DSM 6725 / Z-1320) OX=521460 GN=xylA PE=3 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 3.1e-145
Identity = 252/429 (58.74%), Postives = 315/429 (73.43%), Query Frame = 0

Query: 45  EFFPGIPKIKYEGPDSKKPLAFKWYNADEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGA 104
           ++F  IP++KYEGP S  P AFK+YN DE I GK +KD +RF++A+WHTF  TG DPFG 
Sbjct: 2   KYFKDIPEVKYEGPQSDNPFAFKYYNPDEIIDGKPLKDHLRFAIAYWHTFCATGSDPFGQ 61

Query: 105 ATKYWPWEDGTNSLAMAKRRMRANFEFINKLGVEWWCFHDRDIAPDGQTLKETNANLDAV 164
            T   PW+  +N +  AK R+ A FEF   L V ++CFHDRDIAP+G+ LKE+N NLD +
Sbjct: 62  PTIVRPWDKFSNRMDNAKARVEAAFEFFELLDVPFFCFHDRDIAPEGENLKESNKNLDEI 121

Query: 165 VALAKE-LQGTKIKPLWGTAQLFVHPRYMHGAATSSEVAVYAYAAAQVKKAMEVTHYLGG 224
           V+L KE L+ +K K LWGTA LF HPRY+HGAATS    V+AYAAAQVKKA+EVT  LGG
Sbjct: 122 VSLIKEYLKTSKTKVLWGTANLFSHPRYVHGAATSCNADVFAYAAAQVKKALEVTKELGG 181

Query: 225 ENYVFWGGREGYQSLLNTDMGRELDHLARFLEAAVAHKKKIGFNGTLLIEPKPQEPTKHQ 284
           ENYVFWGGREGY++LLNTDMG ELD+LARFL  AV + K+IGF+G  LIEPKP+EPTKHQ
Sbjct: 182 ENYVFWGGREGYETLLNTDMGLELDNLARFLHMAVEYAKEIGFDGQFLIEPKPKEPTKHQ 241

Query: 285 YDWDAATAANFLRKYGLIDEFKLNIECNHATLSGHSCHHELETARINGLLGNIDANSGDP 344
           YD+D+A    FL+KY L   FKLNIE NHATL+GH  HHEL  ARIN +LG+IDAN GD 
Sbjct: 242 YDFDSAHVYGFLKKYDLDKYFKLNIEVNHATLAGHDFHHELRFARINNMLGSIDANMGDL 301

Query: 345 QVGWDTDQFLTDVGEATMVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDT 404
            +GWDTDQF TDV   T+ M  V+K GG   GG NFDAK+RR S ++EDL I HI+GMD 
Sbjct: 302 LLGWDTDQFPTDVRLTTLAMYEVIKAGGFDKGGLNFDAKVRRGSFELEDLVIGHIAGMDA 361

Query: 405 LARGLRSVKNLIEDGSLMELVRKRYQSFDTEIGAQIEAGKADFEYLEKKALEWGEPKVPS 464
            A+G +    L++DG   + + +RY+S+   IGA+I +G+A+F+ LE+ AL   + +  S
Sbjct: 362 FAKGFKIAYKLVKDGVFDKFIDERYKSYKEGIGAKIVSGEANFKMLEEYALSLDKIENKS 421

Query: 465 AKQELAEMI 473
            KQEL EMI
Sbjct: 422 GKQELLEMI 430

BLAST of MC08g2109 vs. ExPASy Swiss-Prot
Match: Q9KGU2 (Xylose isomerase OS=Caldanaerobacter subterraneus subsp. yonseiensis OX=111519 GN=xylA PE=3 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 2.6e-144
Identity = 250/434 (57.60%), Postives = 316/434 (72.81%), Query Frame = 0

Query: 45  EFFPGIPKIKYEGPDSKKPLAFKWYNADEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGA 104
           E+F  +PK++YEGP S  P AFK+YN DE I GK +K+ +RF+VA+WHTF GTG DPFGA
Sbjct: 2   EYFKNVPKVQYEGPKSNNPYAFKFYNPDEIIDGKPLKEHLRFAVAYWHTFTGTGTDPFGA 61

Query: 105 ATKYWPWEDGTNSLAMAKRRMRANFEFINKLGVEWWCFHDRDIAPDGQTLKETNANLDAV 164
           +T   PW+  ++ + +AK R+ A FEF  KL V ++CFHDRDIAP+G  L+ETN NL  +
Sbjct: 62  STMQRPWDRFSDPMDIAKARVEAAFEFFEKLDVPFFCFHDRDIAPEGDNLRETNKNLGTI 121

Query: 165 VALAKE-LQGTKIKPLWGTAQLFVHPRYMHGAATSSEVAVYAYAAAQVKKAMEVTHYLGG 224
           VA+ K+ L+ +K K LWGTA LF HPRY+HGAATS    V+AYAAAQVKKA+E+T  LGG
Sbjct: 122 VAMIKDYLKTSKAKVLWGTANLFSHPRYVHGAATSCNADVFAYAAAQVKKALEITKELGG 181

Query: 225 ENYVFWGGREGYQSLLNTDMGRELDHLARFLEAAVAHKKKIGFNGTLLIEPKPQEPTKHQ 284
           +NYVFWGGREGY++LLNTD   ELD+LARFL  AV + K+IGF G LLIEPKP+EPTKHQ
Sbjct: 182 QNYVFWGGREGYETLLNTDTELELDNLARFLHMAVEYAKEIGFEGQLLIEPKPKEPTKHQ 241

Query: 285 YDWDAATAANFLRKYGLIDEFKLNIECNHATLSGHSCHHELETARINGLLGNIDANSGDP 344
           YD+DAA    FL+KYGL   FKLNIE NHATL+GH   HEL  ARIN +LG+IDAN  D 
Sbjct: 242 YDFDAAHVYAFLKKYGLDKYFKLNIEVNHATLAGHEFQHELRYARINNILGSIDANMRDM 301

Query: 345 QVGWDTDQFLTDVGEATMVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDT 404
            +GWDTDQF TD+   T+ M  V+K GG   GG NFDAK+RR S + EDLFIAHI+GMD 
Sbjct: 302 LLGWDTDQFPTDIRMTTLAMYEVIKMGGFDKGGLNFDAKVRRASFEPEDLFIAHIAGMDA 361

Query: 405 LARGLRSVKNLIEDGSLMELVRKRYQSFDTEIGAQIEAGKADFEYLEKKALEWGEPKVPS 464
            A+G +    L++DG   + + +RY+S+   IGA+I +G+A+F+ LE+ AL   + +  S
Sbjct: 362 FAKGFKVAYRLVKDGVFDKFIEERYKSYKEGIGAEIVSGRANFKTLEEYALNNPKIENKS 421

Query: 465 AKQELAEMIFQSAL 478
            KQEL E I    L
Sbjct: 422 GKQELLESILNQYL 435

BLAST of MC08g2109 vs. ExPASy Swiss-Prot
Match: P22842 (Xylose isomerase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=xylA PE=3 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 6.5e-143
Identity = 247/434 (56.91%), Postives = 314/434 (72.35%), Query Frame = 0

Query: 45  EFFPGIPKIKYEGPDSKKPLAFKWYNADEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGA 104
           E+F  +P+IKYEGP S  P AFK+YN DE I GK +K+ +RFSVA+WHTF   G DPFGA
Sbjct: 2   EYFKNVPQIKYEGPKSNNPYAFKFYNPDEIIDGKPLKEHLRFSVAYWHTFTANGTDPFGA 61

Query: 105 ATKYWPWEDGTNSLAMAKRRMRANFEFINKLGVEWWCFHDRDIAPDGQTLKETNANLDAV 164
            T   PW+  T+ + +AK R+ A FE   KL V ++CFHDRDIAP+G+TL+ETN NLD +
Sbjct: 62  PTMQRPWDHFTDPMDIAKARVEAAFELFEKLDVPFFCFHDRDIAPEGETLRETNKNLDTI 121

Query: 165 VALAKE-LQGTKIKPLWGTAQLFVHPRYMHGAATSSEVAVYAYAAAQVKKAMEVTHYLGG 224
           VA+ K+ L+ +K K LWGTA LF +PR++HGAATS    V+AYAAAQVKKA+E+T  LGG
Sbjct: 122 VAMIKDYLKTSKTKVLWGTANLFSNPRFVHGAATSCNADVFAYAAAQVKKALEITKELGG 181

Query: 225 ENYVFWGGREGYQSLLNTDMGRELDHLARFLEAAVAHKKKIGFNGTLLIEPKPQEPTKHQ 284
           +NYVFWGGREGY++LLNTDM  ELD+LARFL  AV + ++IGF G  LIEPKP+EPTKHQ
Sbjct: 182 QNYVFWGGREGYETLLNTDMELELDNLARFLHMAVEYAQEIGFEGQFLIEPKPKEPTKHQ 241

Query: 285 YDWDAATAANFLRKYGLIDEFKLNIECNHATLSGHSCHHELETARINGLLGNIDANSGDP 344
           YD+DAA+   FL+KY L   FKLNIE NHATL+GH   HEL  ARIN +LG+IDAN GD 
Sbjct: 242 YDFDAASVHAFLKKYDLDKYFKLNIEANHATLAGHDFQHELRYARINNMLGSIDANMGDM 301

Query: 345 QVGWDTDQFLTDVGEATMVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDT 404
            +GWDTDQ+ TD+   T+ M  V+K GG   GG NFDAK+RR S + EDLF+ HI+GMD 
Sbjct: 302 LLGWDTDQYPTDIRMTTLAMYEVIKMGGFNKGGLNFDAKVRRASFEPEDLFLGHIAGMDA 361

Query: 405 LARGLRSVKNLIEDGSLMELVRKRYQSFDTEIGAQIEAGKADFEYLEKKALEWGEPKVPS 464
            A+G +    L++DG     + +RY+S+   IGA+I +GKA+F+ LE+ AL   + +  S
Sbjct: 362 FAKGFKVAYKLVKDGVFDRFIEERYKSYREGIGAEIVSGKANFKTLEEYALNNPKIENKS 421

Query: 465 AKQELAEMIFQSAL 478
            KQEL E I    L
Sbjct: 422 GKQELLESILNQYL 435

BLAST of MC08g2109 vs. NCBI nr
Match: XP_022153324.1 (xylose isomerase [Momordica charantia] >XP_022153325.1 xylose isomerase [Momordica charantia])

HSP 1 Score: 979 bits (2530), Expect = 0.0
Identity = 477/477 (100.00%), Postives = 477/477 (100.00%), Query Frame = 0

Query: 1   MKSRNVLVLVLCLNVLLLGAIAESPTCPAADLGGECSHSGEWEGEFFPGIPKIKYEGPDS 60
           MKSRNVLVLVLCLNVLLLGAIAESPTCPAADLGGECSHSGEWEGEFFPGIPKIKYEGPDS
Sbjct: 1   MKSRNVLVLVLCLNVLLLGAIAESPTCPAADLGGECSHSGEWEGEFFPGIPKIKYEGPDS 60

Query: 61  KKPLAFKWYNADEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSLAM 120
           KKPLAFKWYNADEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSLAM
Sbjct: 61  KKPLAFKWYNADEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSLAM 120

Query: 121 AKRRMRANFEFINKLGVEWWCFHDRDIAPDGQTLKETNANLDAVVALAKELQGTKIKPLW 180
           AKRRMRANFEFINKLGVEWWCFHDRDIAPDGQTLKETNANLDAVVALAKELQGTKIKPLW
Sbjct: 121 AKRRMRANFEFINKLGVEWWCFHDRDIAPDGQTLKETNANLDAVVALAKELQGTKIKPLW 180

Query: 181 GTAQLFVHPRYMHGAATSSEVAVYAYAAAQVKKAMEVTHYLGGENYVFWGGREGYQSLLN 240
           GTAQLFVHPRYMHGAATSSEVAVYAYAAAQVKKAMEVTHYLGGENYVFWGGREGYQSLLN
Sbjct: 181 GTAQLFVHPRYMHGAATSSEVAVYAYAAAQVKKAMEVTHYLGGENYVFWGGREGYQSLLN 240

Query: 241 TDMGRELDHLARFLEAAVAHKKKIGFNGTLLIEPKPQEPTKHQYDWDAATAANFLRKYGL 300
           TDMGRELDHLARFLEAAVAHKKKIGFNGTLLIEPKPQEPTKHQYDWDAATAANFLRKYGL
Sbjct: 241 TDMGRELDHLARFLEAAVAHKKKIGFNGTLLIEPKPQEPTKHQYDWDAATAANFLRKYGL 300

Query: 301 IDEFKLNIECNHATLSGHSCHHELETARINGLLGNIDANSGDPQVGWDTDQFLTDVGEAT 360
           IDEFKLNIECNHATLSGHSCHHELETARINGLLGNIDANSGDPQVGWDTDQFLTDVGEAT
Sbjct: 301 IDEFKLNIECNHATLSGHSCHHELETARINGLLGNIDANSGDPQVGWDTDQFLTDVGEAT 360

Query: 361 MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLRSVKNLIEDGSL 420
           MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLRSVKNLIEDGSL
Sbjct: 361 MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLRSVKNLIEDGSL 420

Query: 421 MELVRKRYQSFDTEIGAQIEAGKADFEYLEKKALEWGEPKVPSAKQELAEMIFQSAL 477
           MELVRKRYQSFDTEIGAQIEAGKADFEYLEKKALEWGEPKVPSAKQELAEMIFQSAL
Sbjct: 421 MELVRKRYQSFDTEIGAQIEAGKADFEYLEKKALEWGEPKVPSAKQELAEMIFQSAL 477

BLAST of MC08g2109 vs. NCBI nr
Match: XP_022965704.1 (xylose isomerase [Cucurbita maxima])

HSP 1 Score: 904 bits (2336), Expect = 0.0
Identity = 437/477 (91.61%), Postives = 457/477 (95.81%), Query Frame = 0

Query: 1   MKSRNVLVLVLCLNVLLLGAIAESPTCPAADLGGECSHSGEWEGEFFPGIPKIKYEGPDS 60
           MKSR VL L LCLNV LLGAIAE PTCPA D+  ECSHSG+WEGEFFP I KI YEGP S
Sbjct: 1   MKSREVLALFLCLNVFLLGAIAEPPTCPA-DVNNECSHSGDWEGEFFPDISKIVYEGPAS 60

Query: 61  KKPLAFKWYNADEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSLAM 120
           K PLAFKWYNA+EEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNS+AM
Sbjct: 61  KNPLAFKWYNANEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSVAM 120

Query: 121 AKRRMRANFEFINKLGVEWWCFHDRDIAPDGQTLKETNANLDAVVALAKELQGTKIKPLW 180
           AKRRMRANFEFI KLGVE+WCFHDRDIAPDG TL+ETNANLDAVVALAKELQGTKIKPLW
Sbjct: 121 AKRRMRANFEFIKKLGVEFWCFHDRDIAPDGLTLEETNANLDAVVALAKELQGTKIKPLW 180

Query: 181 GTAQLFVHPRYMHGAATSSEVAVYAYAAAQVKKAMEVTHYLGGENYVFWGGREGYQSLLN 240
           GTAQLF+HPRYMHGAATSSEV VYAYAAAQVKKAMEVTH+LGGENYVFWGGREGYQSLLN
Sbjct: 181 GTAQLFMHPRYMHGAATSSEVGVYAYAAAQVKKAMEVTHHLGGENYVFWGGREGYQSLLN 240

Query: 241 TDMGRELDHLARFLEAAVAHKKKIGFNGTLLIEPKPQEPTKHQYDWDAATAANFLRKYGL 300
           TDMGRELDH+ARFL+AA A+KKKIGFNGTLLIEPKPQEPTKHQYDWDAAT+ANFLRKYGL
Sbjct: 241 TDMGRELDHMARFLQAAAAYKKKIGFNGTLLIEPKPQEPTKHQYDWDAATSANFLRKYGL 300

Query: 301 IDEFKLNIECNHATLSGHSCHHELETARINGLLGNIDANSGDPQVGWDTDQFLTDVGEAT 360
           ID+FKLNIECNHATLSGHSCHHELETAR+NG+LGNIDAN+GDPQVGWDTDQFLTD+GEAT
Sbjct: 301 IDDFKLNIECNHATLSGHSCHHELETARLNGILGNIDANTGDPQVGWDTDQFLTDIGEAT 360

Query: 361 MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLRSVKNLIEDGSL 420
           MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLR+V  LIEDGSL
Sbjct: 361 MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLRNVAKLIEDGSL 420

Query: 421 MELVRKRYQSFDTEIGAQIEAGKADFEYLEKKALEWGEPKVPSAKQELAEMIFQSAL 477
            ELVRKRYQSFDTEIGAQIEAGKADFEYLEKKA+EWGEPKVPSAKQELAEM+FQSAL
Sbjct: 421 TELVRKRYQSFDTEIGAQIEAGKADFEYLEKKAMEWGEPKVPSAKQELAEMLFQSAL 476

BLAST of MC08g2109 vs. NCBI nr
Match: XP_023537255.1 (xylose isomerase isoform X1 [Cucurbita pepo subsp. pepo] >XP_023537256.1 xylose isomerase isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 904 bits (2335), Expect = 0.0
Identity = 437/477 (91.61%), Postives = 456/477 (95.60%), Query Frame = 0

Query: 1   MKSRNVLVLVLCLNVLLLGAIAESPTCPAADLGGECSHSGEWEGEFFPGIPKIKYEGPDS 60
           MKSR VL L LCLNV LLGAIAE PTCPA D   ECSHSG+WEGEFFPGI KI YEGP S
Sbjct: 17  MKSREVLALFLCLNVFLLGAIAEPPTCPA-DANNECSHSGDWEGEFFPGISKIVYEGPAS 76

Query: 61  KKPLAFKWYNADEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSLAM 120
           K PLAFKWYNA+EEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNS+AM
Sbjct: 77  KNPLAFKWYNANEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSVAM 136

Query: 121 AKRRMRANFEFINKLGVEWWCFHDRDIAPDGQTLKETNANLDAVVALAKELQGTKIKPLW 180
           AKRRMRANFEFI KLGVE+WCFHDRDIAPD  TL+ETNANLDAVVALAKELQGTKIKPLW
Sbjct: 137 AKRRMRANFEFIKKLGVEFWCFHDRDIAPDALTLEETNANLDAVVALAKELQGTKIKPLW 196

Query: 181 GTAQLFVHPRYMHGAATSSEVAVYAYAAAQVKKAMEVTHYLGGENYVFWGGREGYQSLLN 240
           GTAQLF+HPRYMHGAATSSEV VYAYAAAQVKKAMEVTH+LGGENYVFWGGREGYQSLLN
Sbjct: 197 GTAQLFMHPRYMHGAATSSEVGVYAYAAAQVKKAMEVTHHLGGENYVFWGGREGYQSLLN 256

Query: 241 TDMGRELDHLARFLEAAVAHKKKIGFNGTLLIEPKPQEPTKHQYDWDAATAANFLRKYGL 300
           TDMGRELDH+ARFL+AA A+KKKIGFNGTLLIEPKPQEPTKHQYDWDAAT+ANFLRKYGL
Sbjct: 257 TDMGRELDHMARFLQAAAAYKKKIGFNGTLLIEPKPQEPTKHQYDWDAATSANFLRKYGL 316

Query: 301 IDEFKLNIECNHATLSGHSCHHELETARINGLLGNIDANSGDPQVGWDTDQFLTDVGEAT 360
           ID+FKLNIECNHATLSGHSCHHELETAR+NG+LGNIDAN+GDPQVGWDTDQFLTD+GEAT
Sbjct: 317 IDDFKLNIECNHATLSGHSCHHELETARLNGILGNIDANTGDPQVGWDTDQFLTDIGEAT 376

Query: 361 MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLRSVKNLIEDGSL 420
           MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLR+V  LIEDGSL
Sbjct: 377 MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLRNVAKLIEDGSL 436

Query: 421 MELVRKRYQSFDTEIGAQIEAGKADFEYLEKKALEWGEPKVPSAKQELAEMIFQSAL 477
            ELVRKRYQSFDTEIGAQIEAGKADFEYLEKKA+EWGEPKVPSAKQELAEM+FQSAL
Sbjct: 437 TELVRKRYQSFDTEIGAQIEAGKADFEYLEKKAMEWGEPKVPSAKQELAEMLFQSAL 492

BLAST of MC08g2109 vs. NCBI nr
Match: XP_023537257.1 (xylose isomerase isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 904 bits (2335), Expect = 0.0
Identity = 437/477 (91.61%), Postives = 456/477 (95.60%), Query Frame = 0

Query: 1   MKSRNVLVLVLCLNVLLLGAIAESPTCPAADLGGECSHSGEWEGEFFPGIPKIKYEGPDS 60
           MKSR VL L LCLNV LLGAIAE PTCPA D   ECSHSG+WEGEFFPGI KI YEGP S
Sbjct: 1   MKSREVLALFLCLNVFLLGAIAEPPTCPA-DANNECSHSGDWEGEFFPGISKIVYEGPAS 60

Query: 61  KKPLAFKWYNADEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSLAM 120
           K PLAFKWYNA+EEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNS+AM
Sbjct: 61  KNPLAFKWYNANEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSVAM 120

Query: 121 AKRRMRANFEFINKLGVEWWCFHDRDIAPDGQTLKETNANLDAVVALAKELQGTKIKPLW 180
           AKRRMRANFEFI KLGVE+WCFHDRDIAPD  TL+ETNANLDAVVALAKELQGTKIKPLW
Sbjct: 121 AKRRMRANFEFIKKLGVEFWCFHDRDIAPDALTLEETNANLDAVVALAKELQGTKIKPLW 180

Query: 181 GTAQLFVHPRYMHGAATSSEVAVYAYAAAQVKKAMEVTHYLGGENYVFWGGREGYQSLLN 240
           GTAQLF+HPRYMHGAATSSEV VYAYAAAQVKKAMEVTH+LGGENYVFWGGREGYQSLLN
Sbjct: 181 GTAQLFMHPRYMHGAATSSEVGVYAYAAAQVKKAMEVTHHLGGENYVFWGGREGYQSLLN 240

Query: 241 TDMGRELDHLARFLEAAVAHKKKIGFNGTLLIEPKPQEPTKHQYDWDAATAANFLRKYGL 300
           TDMGRELDH+ARFL+AA A+KKKIGFNGTLLIEPKPQEPTKHQYDWDAAT+ANFLRKYGL
Sbjct: 241 TDMGRELDHMARFLQAAAAYKKKIGFNGTLLIEPKPQEPTKHQYDWDAATSANFLRKYGL 300

Query: 301 IDEFKLNIECNHATLSGHSCHHELETARINGLLGNIDANSGDPQVGWDTDQFLTDVGEAT 360
           ID+FKLNIECNHATLSGHSCHHELETAR+NG+LGNIDAN+GDPQVGWDTDQFLTD+GEAT
Sbjct: 301 IDDFKLNIECNHATLSGHSCHHELETARLNGILGNIDANTGDPQVGWDTDQFLTDIGEAT 360

Query: 361 MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLRSVKNLIEDGSL 420
           MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLR+V  LIEDGSL
Sbjct: 361 MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLRNVAKLIEDGSL 420

Query: 421 MELVRKRYQSFDTEIGAQIEAGKADFEYLEKKALEWGEPKVPSAKQELAEMIFQSAL 477
            ELVRKRYQSFDTEIGAQIEAGKADFEYLEKKA+EWGEPKVPSAKQELAEM+FQSAL
Sbjct: 421 TELVRKRYQSFDTEIGAQIEAGKADFEYLEKKAMEWGEPKVPSAKQELAEMLFQSAL 476

BLAST of MC08g2109 vs. NCBI nr
Match: XP_022937824.1 (xylose isomerase isoform X1 [Cucurbita moschata])

HSP 1 Score: 903 bits (2334), Expect = 0.0
Identity = 437/477 (91.61%), Postives = 456/477 (95.60%), Query Frame = 0

Query: 1   MKSRNVLVLVLCLNVLLLGAIAESPTCPAADLGGECSHSGEWEGEFFPGIPKIKYEGPDS 60
           MKSR VL L LCLNV LLGAIAE PTCPA D   ECSHSG+WEGEFFPGI KI YEGP S
Sbjct: 17  MKSREVLALFLCLNVFLLGAIAEPPTCPA-DANNECSHSGDWEGEFFPGINKIVYEGPAS 76

Query: 61  KKPLAFKWYNADEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSLAM 120
           K PLAFKWYNA+EEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNS+AM
Sbjct: 77  KNPLAFKWYNANEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSVAM 136

Query: 121 AKRRMRANFEFINKLGVEWWCFHDRDIAPDGQTLKETNANLDAVVALAKELQGTKIKPLW 180
           AKRRMRANFEFI KLGVE+WCFHDRDIAPD  TL+ETNANLDAVVALAKELQGTKIKPLW
Sbjct: 137 AKRRMRANFEFIKKLGVEFWCFHDRDIAPDALTLEETNANLDAVVALAKELQGTKIKPLW 196

Query: 181 GTAQLFVHPRYMHGAATSSEVAVYAYAAAQVKKAMEVTHYLGGENYVFWGGREGYQSLLN 240
           GTAQLF+HPRYMHGAATSSEV VYAYAAAQVKKAMEVTH+LGGENYVFWGGREGYQSLLN
Sbjct: 197 GTAQLFMHPRYMHGAATSSEVGVYAYAAAQVKKAMEVTHHLGGENYVFWGGREGYQSLLN 256

Query: 241 TDMGRELDHLARFLEAAVAHKKKIGFNGTLLIEPKPQEPTKHQYDWDAATAANFLRKYGL 300
           TDMGRELDH+ARFL+AA A+KKKIGFNGTLLIEPKPQEPTKHQYDWDAAT+ANFLRKYGL
Sbjct: 257 TDMGRELDHMARFLQAAAAYKKKIGFNGTLLIEPKPQEPTKHQYDWDAATSANFLRKYGL 316

Query: 301 IDEFKLNIECNHATLSGHSCHHELETARINGLLGNIDANSGDPQVGWDTDQFLTDVGEAT 360
           ID+FKLNIECNHATLSGHSCHHELETAR+NG+LGNIDAN+GDPQVGWDTDQFLTD+GEAT
Sbjct: 317 IDDFKLNIECNHATLSGHSCHHELETARLNGILGNIDANTGDPQVGWDTDQFLTDIGEAT 376

Query: 361 MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLRSVKNLIEDGSL 420
           MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLR+V  LIEDGSL
Sbjct: 377 MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLRNVAKLIEDGSL 436

Query: 421 MELVRKRYQSFDTEIGAQIEAGKADFEYLEKKALEWGEPKVPSAKQELAEMIFQSAL 477
            ELVRKRYQSFDTEIGAQIEAGKADFEYLEKKA+EWGEPKVPSAKQELAEM+FQSAL
Sbjct: 437 TELVRKRYQSFDTEIGAQIEAGKADFEYLEKKAMEWGEPKVPSAKQELAEMLFQSAL 492

BLAST of MC08g2109 vs. ExPASy TrEMBL
Match: A0A6J1DIR0 (Xylose isomerase OS=Momordica charantia OX=3673 GN=LOC111020849 PE=3 SV=1)

HSP 1 Score: 979 bits (2530), Expect = 0.0
Identity = 477/477 (100.00%), Postives = 477/477 (100.00%), Query Frame = 0

Query: 1   MKSRNVLVLVLCLNVLLLGAIAESPTCPAADLGGECSHSGEWEGEFFPGIPKIKYEGPDS 60
           MKSRNVLVLVLCLNVLLLGAIAESPTCPAADLGGECSHSGEWEGEFFPGIPKIKYEGPDS
Sbjct: 1   MKSRNVLVLVLCLNVLLLGAIAESPTCPAADLGGECSHSGEWEGEFFPGIPKIKYEGPDS 60

Query: 61  KKPLAFKWYNADEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSLAM 120
           KKPLAFKWYNADEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSLAM
Sbjct: 61  KKPLAFKWYNADEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSLAM 120

Query: 121 AKRRMRANFEFINKLGVEWWCFHDRDIAPDGQTLKETNANLDAVVALAKELQGTKIKPLW 180
           AKRRMRANFEFINKLGVEWWCFHDRDIAPDGQTLKETNANLDAVVALAKELQGTKIKPLW
Sbjct: 121 AKRRMRANFEFINKLGVEWWCFHDRDIAPDGQTLKETNANLDAVVALAKELQGTKIKPLW 180

Query: 181 GTAQLFVHPRYMHGAATSSEVAVYAYAAAQVKKAMEVTHYLGGENYVFWGGREGYQSLLN 240
           GTAQLFVHPRYMHGAATSSEVAVYAYAAAQVKKAMEVTHYLGGENYVFWGGREGYQSLLN
Sbjct: 181 GTAQLFVHPRYMHGAATSSEVAVYAYAAAQVKKAMEVTHYLGGENYVFWGGREGYQSLLN 240

Query: 241 TDMGRELDHLARFLEAAVAHKKKIGFNGTLLIEPKPQEPTKHQYDWDAATAANFLRKYGL 300
           TDMGRELDHLARFLEAAVAHKKKIGFNGTLLIEPKPQEPTKHQYDWDAATAANFLRKYGL
Sbjct: 241 TDMGRELDHLARFLEAAVAHKKKIGFNGTLLIEPKPQEPTKHQYDWDAATAANFLRKYGL 300

Query: 301 IDEFKLNIECNHATLSGHSCHHELETARINGLLGNIDANSGDPQVGWDTDQFLTDVGEAT 360
           IDEFKLNIECNHATLSGHSCHHELETARINGLLGNIDANSGDPQVGWDTDQFLTDVGEAT
Sbjct: 301 IDEFKLNIECNHATLSGHSCHHELETARINGLLGNIDANSGDPQVGWDTDQFLTDVGEAT 360

Query: 361 MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLRSVKNLIEDGSL 420
           MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLRSVKNLIEDGSL
Sbjct: 361 MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLRSVKNLIEDGSL 420

Query: 421 MELVRKRYQSFDTEIGAQIEAGKADFEYLEKKALEWGEPKVPSAKQELAEMIFQSAL 477
           MELVRKRYQSFDTEIGAQIEAGKADFEYLEKKALEWGEPKVPSAKQELAEMIFQSAL
Sbjct: 421 MELVRKRYQSFDTEIGAQIEAGKADFEYLEKKALEWGEPKVPSAKQELAEMIFQSAL 477

BLAST of MC08g2109 vs. ExPASy TrEMBL
Match: A0A6J1HRR2 (Xylose isomerase OS=Cucurbita maxima OX=3661 GN=LOC111465524 PE=3 SV=1)

HSP 1 Score: 904 bits (2336), Expect = 0.0
Identity = 437/477 (91.61%), Postives = 457/477 (95.81%), Query Frame = 0

Query: 1   MKSRNVLVLVLCLNVLLLGAIAESPTCPAADLGGECSHSGEWEGEFFPGIPKIKYEGPDS 60
           MKSR VL L LCLNV LLGAIAE PTCPA D+  ECSHSG+WEGEFFP I KI YEGP S
Sbjct: 1   MKSREVLALFLCLNVFLLGAIAEPPTCPA-DVNNECSHSGDWEGEFFPDISKIVYEGPAS 60

Query: 61  KKPLAFKWYNADEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSLAM 120
           K PLAFKWYNA+EEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNS+AM
Sbjct: 61  KNPLAFKWYNANEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSVAM 120

Query: 121 AKRRMRANFEFINKLGVEWWCFHDRDIAPDGQTLKETNANLDAVVALAKELQGTKIKPLW 180
           AKRRMRANFEFI KLGVE+WCFHDRDIAPDG TL+ETNANLDAVVALAKELQGTKIKPLW
Sbjct: 121 AKRRMRANFEFIKKLGVEFWCFHDRDIAPDGLTLEETNANLDAVVALAKELQGTKIKPLW 180

Query: 181 GTAQLFVHPRYMHGAATSSEVAVYAYAAAQVKKAMEVTHYLGGENYVFWGGREGYQSLLN 240
           GTAQLF+HPRYMHGAATSSEV VYAYAAAQVKKAMEVTH+LGGENYVFWGGREGYQSLLN
Sbjct: 181 GTAQLFMHPRYMHGAATSSEVGVYAYAAAQVKKAMEVTHHLGGENYVFWGGREGYQSLLN 240

Query: 241 TDMGRELDHLARFLEAAVAHKKKIGFNGTLLIEPKPQEPTKHQYDWDAATAANFLRKYGL 300
           TDMGRELDH+ARFL+AA A+KKKIGFNGTLLIEPKPQEPTKHQYDWDAAT+ANFLRKYGL
Sbjct: 241 TDMGRELDHMARFLQAAAAYKKKIGFNGTLLIEPKPQEPTKHQYDWDAATSANFLRKYGL 300

Query: 301 IDEFKLNIECNHATLSGHSCHHELETARINGLLGNIDANSGDPQVGWDTDQFLTDVGEAT 360
           ID+FKLNIECNHATLSGHSCHHELETAR+NG+LGNIDAN+GDPQVGWDTDQFLTD+GEAT
Sbjct: 301 IDDFKLNIECNHATLSGHSCHHELETARLNGILGNIDANTGDPQVGWDTDQFLTDIGEAT 360

Query: 361 MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLRSVKNLIEDGSL 420
           MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLR+V  LIEDGSL
Sbjct: 361 MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLRNVAKLIEDGSL 420

Query: 421 MELVRKRYQSFDTEIGAQIEAGKADFEYLEKKALEWGEPKVPSAKQELAEMIFQSAL 477
            ELVRKRYQSFDTEIGAQIEAGKADFEYLEKKA+EWGEPKVPSAKQELAEM+FQSAL
Sbjct: 421 TELVRKRYQSFDTEIGAQIEAGKADFEYLEKKAMEWGEPKVPSAKQELAEMLFQSAL 476

BLAST of MC08g2109 vs. ExPASy TrEMBL
Match: A0A6J1FCB2 (Xylose isomerase OS=Cucurbita moschata OX=3662 GN=LOC111444098 PE=3 SV=1)

HSP 1 Score: 903 bits (2334), Expect = 0.0
Identity = 437/477 (91.61%), Postives = 456/477 (95.60%), Query Frame = 0

Query: 1   MKSRNVLVLVLCLNVLLLGAIAESPTCPAADLGGECSHSGEWEGEFFPGIPKIKYEGPDS 60
           MKSR VL L LCLNV LLGAIAE PTCPA D   ECSHSG+WEGEFFPGI KI YEGP S
Sbjct: 17  MKSREVLALFLCLNVFLLGAIAEPPTCPA-DANNECSHSGDWEGEFFPGINKIVYEGPAS 76

Query: 61  KKPLAFKWYNADEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSLAM 120
           K PLAFKWYNA+EEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNS+AM
Sbjct: 77  KNPLAFKWYNANEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSVAM 136

Query: 121 AKRRMRANFEFINKLGVEWWCFHDRDIAPDGQTLKETNANLDAVVALAKELQGTKIKPLW 180
           AKRRMRANFEFI KLGVE+WCFHDRDIAPD  TL+ETNANLDAVVALAKELQGTKIKPLW
Sbjct: 137 AKRRMRANFEFIKKLGVEFWCFHDRDIAPDALTLEETNANLDAVVALAKELQGTKIKPLW 196

Query: 181 GTAQLFVHPRYMHGAATSSEVAVYAYAAAQVKKAMEVTHYLGGENYVFWGGREGYQSLLN 240
           GTAQLF+HPRYMHGAATSSEV VYAYAAAQVKKAMEVTH+LGGENYVFWGGREGYQSLLN
Sbjct: 197 GTAQLFMHPRYMHGAATSSEVGVYAYAAAQVKKAMEVTHHLGGENYVFWGGREGYQSLLN 256

Query: 241 TDMGRELDHLARFLEAAVAHKKKIGFNGTLLIEPKPQEPTKHQYDWDAATAANFLRKYGL 300
           TDMGRELDH+ARFL+AA A+KKKIGFNGTLLIEPKPQEPTKHQYDWDAAT+ANFLRKYGL
Sbjct: 257 TDMGRELDHMARFLQAAAAYKKKIGFNGTLLIEPKPQEPTKHQYDWDAATSANFLRKYGL 316

Query: 301 IDEFKLNIECNHATLSGHSCHHELETARINGLLGNIDANSGDPQVGWDTDQFLTDVGEAT 360
           ID+FKLNIECNHATLSGHSCHHELETAR+NG+LGNIDAN+GDPQVGWDTDQFLTD+GEAT
Sbjct: 317 IDDFKLNIECNHATLSGHSCHHELETARLNGILGNIDANTGDPQVGWDTDQFLTDIGEAT 376

Query: 361 MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLRSVKNLIEDGSL 420
           MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLR+V  LIEDGSL
Sbjct: 377 MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLRNVAKLIEDGSL 436

Query: 421 MELVRKRYQSFDTEIGAQIEAGKADFEYLEKKALEWGEPKVPSAKQELAEMIFQSAL 477
            ELVRKRYQSFDTEIGAQIEAGKADFEYLEKKA+EWGEPKVPSAKQELAEM+FQSAL
Sbjct: 437 TELVRKRYQSFDTEIGAQIEAGKADFEYLEKKAMEWGEPKVPSAKQELAEMLFQSAL 492

BLAST of MC08g2109 vs. ExPASy TrEMBL
Match: A0A6J1FHW4 (Xylose isomerase OS=Cucurbita moschata OX=3662 GN=LOC111444098 PE=3 SV=1)

HSP 1 Score: 903 bits (2334), Expect = 0.0
Identity = 437/477 (91.61%), Postives = 456/477 (95.60%), Query Frame = 0

Query: 1   MKSRNVLVLVLCLNVLLLGAIAESPTCPAADLGGECSHSGEWEGEFFPGIPKIKYEGPDS 60
           MKSR VL L LCLNV LLGAIAE PTCPA D   ECSHSG+WEGEFFPGI KI YEGP S
Sbjct: 1   MKSREVLALFLCLNVFLLGAIAEPPTCPA-DANNECSHSGDWEGEFFPGINKIVYEGPAS 60

Query: 61  KKPLAFKWYNADEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSLAM 120
           K PLAFKWYNA+EEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNS+AM
Sbjct: 61  KNPLAFKWYNANEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSVAM 120

Query: 121 AKRRMRANFEFINKLGVEWWCFHDRDIAPDGQTLKETNANLDAVVALAKELQGTKIKPLW 180
           AKRRMRANFEFI KLGVE+WCFHDRDIAPD  TL+ETNANLDAVVALAKELQGTKIKPLW
Sbjct: 121 AKRRMRANFEFIKKLGVEFWCFHDRDIAPDALTLEETNANLDAVVALAKELQGTKIKPLW 180

Query: 181 GTAQLFVHPRYMHGAATSSEVAVYAYAAAQVKKAMEVTHYLGGENYVFWGGREGYQSLLN 240
           GTAQLF+HPRYMHGAATSSEV VYAYAAAQVKKAMEVTH+LGGENYVFWGGREGYQSLLN
Sbjct: 181 GTAQLFMHPRYMHGAATSSEVGVYAYAAAQVKKAMEVTHHLGGENYVFWGGREGYQSLLN 240

Query: 241 TDMGRELDHLARFLEAAVAHKKKIGFNGTLLIEPKPQEPTKHQYDWDAATAANFLRKYGL 300
           TDMGRELDH+ARFL+AA A+KKKIGFNGTLLIEPKPQEPTKHQYDWDAAT+ANFLRKYGL
Sbjct: 241 TDMGRELDHMARFLQAAAAYKKKIGFNGTLLIEPKPQEPTKHQYDWDAATSANFLRKYGL 300

Query: 301 IDEFKLNIECNHATLSGHSCHHELETARINGLLGNIDANSGDPQVGWDTDQFLTDVGEAT 360
           ID+FKLNIECNHATLSGHSCHHELETAR+NG+LGNIDAN+GDPQVGWDTDQFLTD+GEAT
Sbjct: 301 IDDFKLNIECNHATLSGHSCHHELETARLNGILGNIDANTGDPQVGWDTDQFLTDIGEAT 360

Query: 361 MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLRSVKNLIEDGSL 420
           MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLR+V  LIEDGSL
Sbjct: 361 MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLRNVAKLIEDGSL 420

Query: 421 MELVRKRYQSFDTEIGAQIEAGKADFEYLEKKALEWGEPKVPSAKQELAEMIFQSAL 477
            ELVRKRYQSFDTEIGAQIEAGKADFEYLEKKA+EWGEPKVPSAKQELAEM+FQSAL
Sbjct: 421 TELVRKRYQSFDTEIGAQIEAGKADFEYLEKKAMEWGEPKVPSAKQELAEMLFQSAL 476

BLAST of MC08g2109 vs. ExPASy TrEMBL
Match: A0A0A0LK73 (Xylose isomerase OS=Cucumis sativus OX=3659 GN=Csa_2G049960 PE=3 SV=1)

HSP 1 Score: 877 bits (2265), Expect = 0.0
Identity = 419/477 (87.84%), Postives = 448/477 (93.92%), Query Frame = 0

Query: 1   MKSRNVLVLVLCLNVLLLGAIAESPTCPAADLGGECSHSGEWEGEFFPGIPKIKYEGPDS 60
           MKSR + VL+LCL   +LGAIA S TCPA DL  ECSHSG+WEGEFFPGIPKIKYEGP S
Sbjct: 1   MKSREISVLLLCLLGFVLGAIANSHTCPA-DLSSECSHSGDWEGEFFPGIPKIKYEGPTS 60

Query: 61  KKPLAFKWYNADEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSLAM 120
           K PLA+KWYNADEEILGKKMKDWMRFSVA+WHTFRGTG DPFGAATK+WPWEDGTNS+AM
Sbjct: 61  KNPLAYKWYNADEEILGKKMKDWMRFSVAYWHTFRGTGADPFGAATKHWPWEDGTNSVAM 120

Query: 121 AKRRMRANFEFINKLGVEWWCFHDRDIAPDGQTLKETNANLDAVVALAKELQGTKIKPLW 180
           AKRRMRANFEFINKLGVEWWCFHDRDIAPD  TL+ETNAN D VVA+AKELQGTKIKPLW
Sbjct: 121 AKRRMRANFEFINKLGVEWWCFHDRDIAPDAPTLEETNANFDEVVAVAKELQGTKIKPLW 180

Query: 181 GTAQLFVHPRYMHGAATSSEVAVYAYAAAQVKKAMEVTHYLGGENYVFWGGREGYQSLLN 240
            TAQLF+HPRYMHG ATSSEV VYAYAAAQVKKAMEV HYLG ENYVFWGGREGYQ+LLN
Sbjct: 181 VTAQLFMHPRYMHGGATSSEVGVYAYAAAQVKKAMEVAHYLGAENYVFWGGREGYQTLLN 240

Query: 241 TDMGRELDHLARFLEAAVAHKKKIGFNGTLLIEPKPQEPTKHQYDWDAATAANFLRKYGL 300
           TDMGRELDH+ARF +AAVA+KKKIGFNGTLLIEPKPQEPTKHQYDWDAAT+ANFLRKYGL
Sbjct: 241 TDMGRELDHMARFFQAAVAYKKKIGFNGTLLIEPKPQEPTKHQYDWDAATSANFLRKYGL 300

Query: 301 IDEFKLNIECNHATLSGHSCHHELETARINGLLGNIDANSGDPQVGWDTDQFLTDVGEAT 360
           IDEF+LNIECNH TLSGHSCHH+LETAR+NG+LG+IDAN+GDPQVGWDTDQF+TD+ EAT
Sbjct: 301 IDEFQLNIECNHVTLSGHSCHHDLETARLNGILGSIDANTGDPQVGWDTDQFMTDIAEAT 360

Query: 361 MVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLRSVKNLIEDGSL 420
           MVMLSVV+NGGLAPGGFNFDAKLRRESTDVEDLFIAHI GMDTLARGLR+V  L+EDGSL
Sbjct: 361 MVMLSVVRNGGLAPGGFNFDAKLRRESTDVEDLFIAHIGGMDTLARGLRNVAKLLEDGSL 420

Query: 421 MELVRKRYQSFDTEIGAQIEAGKADFEYLEKKALEWGEPKVPSAKQELAEMIFQSAL 477
            ELVRKRY+SFDTEIGAQIEAGKADFE+LEKKALEWGEPKVPSAKQELAEMIFQSAL
Sbjct: 421 TELVRKRYESFDTEIGAQIEAGKADFEFLEKKALEWGEPKVPSAKQELAEMIFQSAL 476

BLAST of MC08g2109 vs. TAIR 10
Match: AT5G57655.2 (xylose isomerase family protein )

HSP 1 Score: 841.6 bits (2173), Expect = 3.0e-244
Identity = 398/478 (83.26%), Postives = 435/478 (91.00%), Query Frame = 0

Query: 1   MKSRNVLVLVLCLNVLLLGAIAESPTCPAADLGGECSHSGEWEGEFFPGIPKIKYEGPDS 60
           MK     +L+LC         A+ PTCP ADLGG+CS S +W+G+FFP IPKIKYEGP S
Sbjct: 1   MKKVEFFMLLLCFIAASSLVSADPPTCP-ADLGGKCSDSDDWQGDFFPEIPKIKYEGPSS 60

Query: 61  KKPLAFKWYNADEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSLAM 120
           K PLA++WYNA+EEILGKKMKDW RFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNS++M
Sbjct: 61  KNPLAYRWYNAEEEILGKKMKDWFRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSVSM 120

Query: 121 AKRRMRANFEFINKLGVEWWCFHDRDIAPDGQTLKETNANLDAVVALAKELQ-GTKIKPL 180
           AKRRMRANFEF+ KLGV+WWCFHDRDIAPDG TL+E+N NLD V+ LAKELQ G+KIKPL
Sbjct: 121 AKRRMRANFEFLKKLGVDWWCFHDRDIAPDGTTLEESNKNLDEVIELAKELQKGSKIKPL 180

Query: 181 WGTAQLFVHPRYMHGAATSSEVAVYAYAAAQVKKAMEVTHYLGGENYVFWGGREGYQSLL 240
           WGTAQLF+HPRYMHG ATSSEV VYAYAAAQVKKAMEVTHYLGGENYVFWGGREGYQ+LL
Sbjct: 181 WGTAQLFLHPRYMHGGATSSEVGVYAYAAAQVKKAMEVTHYLGGENYVFWGGREGYQTLL 240

Query: 241 NTDMGRELDHLARFLEAAVAHKKKIGFNGTLLIEPKPQEPTKHQYDWDAATAANFLRKYG 300
           NTDMGRELDHLARF EAAVA+KKKIGF GTLLIEPKPQEPTKHQYDWDAATAANFLRKYG
Sbjct: 241 NTDMGRELDHLARFFEAAVAYKKKIGFKGTLLIEPKPQEPTKHQYDWDAATAANFLRKYG 300

Query: 301 LIDEFKLNIECNHATLSGHSCHHELETARINGLLGNIDANSGDPQVGWDTDQFLTDVGEA 360
           LIDEFKLNIECNHATLSGH+CHHELETARINGLLGNIDAN+GD Q GWDTDQFLTDVGEA
Sbjct: 301 LIDEFKLNIECNHATLSGHTCHHELETARINGLLGNIDANTGDAQTGWDTDQFLTDVGEA 360

Query: 361 TMVMLSVVKNGGLAPGGFNFDAKLRRESTDVEDLFIAHISGMDTLARGLRSVKNLIEDGS 420
           TMVM+SV+KNGG+APGGFNFDAKLRRESTDVEDLFIAHISGMDT+ARGLR+   ++E+GS
Sbjct: 361 TMVMMSVIKNGGIAPGGFNFDAKLRRESTDVEDLFIAHISGMDTMARGLRNAVKILEEGS 420

Query: 421 LMELVRKRYQSFDTEIGAQIEAGKADFEYLEKKALEWGEPKVPSAKQELAEMIFQSAL 478
           L ELVRKRY ++D+E+G QIE GKADFEYLEKKA E+GEPKV SAKQELAEMIFQSA+
Sbjct: 421 LSELVRKRYATWDSELGKQIEEGKADFEYLEKKAKEFGEPKVSSAKQELAEMIFQSAM 477

BLAST of MC08g2109 vs. TAIR 10
Match: AT5G57655.1 (xylose isomerase family protein )

HSP 1 Score: 463.4 bits (1191), Expect = 2.2e-130
Identity = 216/269 (80.30%), Postives = 237/269 (88.10%), Query Frame = 0

Query: 1   MKSRNVLVLVLCLNVLLLGAIAESPTCPAADLGGECSHSGEWEGEFFPGIPKIKYEGPDS 60
           MK     +L+LC         A+ PTCP ADLGG+CS S +W+G+FFP IPKIKYEGP S
Sbjct: 1   MKKVEFFMLLLCFIAASSLVSADPPTCP-ADLGGKCSDSDDWQGDFFPEIPKIKYEGPSS 60

Query: 61  KKPLAFKWYNADEEILGKKMKDWMRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSLAM 120
           K PLA++WYNA+EEILGKKMKDW RFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNS++M
Sbjct: 61  KNPLAYRWYNAEEEILGKKMKDWFRFSVAFWHTFRGTGGDPFGAATKYWPWEDGTNSVSM 120

Query: 121 AKRRMRANFEFINKLGVEWWCFHDRDIAPDGQTLKETNANLDAVVALAKELQ-GTKIKPL 180
           AKRRMRANFEF+ KLGV+WWCFHDRDIAPDG TL+E+N NLD V+ LAKELQ G+KIKPL
Sbjct: 121 AKRRMRANFEFLKKLGVDWWCFHDRDIAPDGTTLEESNKNLDEVIELAKELQKGSKIKPL 180

Query: 181 WGTAQLFVHPRYMHGAATSSEVAVYAYAAAQVKKAMEVTHYLGGENYVFWGGREGYQSLL 240
           WGTAQLF+HPRYMHG ATSSEV VYAYAAAQVKKAMEVTHYLGGENYVFWGGREGYQ+LL
Sbjct: 181 WGTAQLFLHPRYMHGGATSSEVGVYAYAAAQVKKAMEVTHYLGGENYVFWGGREGYQTLL 240

Query: 241 NTDMGRELDHLARFLEAAVAHKKKIGFNG 269
           NTDMGRELDHLARF EAAVA+KKKIGF G
Sbjct: 241 NTDMGRELDHLARFFEAAVAYKKKIGFKG 268