MC08g2103 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g2103 vs. ExPASy Swiss-Prot
Match: F4JQ95 (Diacylglycerol kinase 7 OS=Arabidopsis thaliana OX=3702 GN=DGK7 PE=1 SV=1)

HSP 1 Score: 676.4 bits (1744), Expect = 2.4e-193
Identity = 323/499 (64.73%), Postives = 394/499 (78.96%), Query Frame = 0

Query: 1   MESLTTNGGEDSST-----RIAARSSMIESLKGCG---LTGIHVDKEELRKKLQMPQYLR 60
           ME    + GE S+T     R +A++    +++GCG   L  + +DKEELR +L MP+YLR
Sbjct: 1   MEETPRSVGEASTTNFVAARPSAKTDDAVTMRGCGFANLALVGIDKEELRGRLAMPEYLR 60

Query: 61  FAMRDSIRLQDPAAGESRLPAANSLSGSAFAPEINEPPESPMVVFINPRSGGRHGRLLKE 120
            AMRD I+ +D       L     L G A A   +  P +PMVVFINP+SGGRHG +LKE
Sbjct: 61  IAMRDCIKRKDSTEISDHL----LLPGGAAA---DMAPHAPMVVFINPKSGGRHGPVLKE 120

Query: 121 RLQMLISEEQVFDLTDVKPHEFVKYGLKCLELLAAIGDACAKETRDKMRVMVAGGDGTVG 180
           RLQ L++EEQVFDLT+VKPHEFV+YGL CL+ LAA GD CA+E R+K+R+MVAGGDGTVG
Sbjct: 121 RLQQLMTEEQVFDLTEVKPHEFVRYGLGCLDTLAAKGDECARECREKIRIMVAGGDGTVG 180

Query: 181 WVLGCLLELEKQGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGR 240
           WVLGCL EL K G+  +PPVGVIPLGTGNDLSR+F WGGSFPFAW+SA+KR+L RAT G 
Sbjct: 181 WVLGCLGELHKDGKSHIPPVGVIPLGTGNDLSRSFSWGGSFPFAWRSAMKRTLHRATLGS 240

Query: 241 VCKLDSWNVLLSAPNSINVELPHCMRPT-EDGALDESFEIKEASSEKASCHGGVFYNYFS 300
           + +LDSW +++S P+   V+ P+ ++PT E+ ALD++ +       KA  + GVFYNYFS
Sbjct: 241 IARLDSWKIVVSMPSGEVVDPPYSLKPTIEETALDQALDADGDVPPKAKSYEGVFYNYFS 300

Query: 301 IGMDAQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILR 360
           IGMDAQVAYGFHHLRNEKPYLAQGP+ NK+IYS YSC QGWF TPC ++P LRGL+NI++
Sbjct: 301 IGMDAQVAYGFHHLRNEKPYLAQGPVTNKIIYSSYSCTQGWFCTPCVNNPALRGLRNIMK 360

Query: 361 MHVKKVNCSDWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGL 420
           +H+KK NCS+WE++ +P SVRS+V LNL NYGSGRHPWGNL P+Y+EKRGFVEA  DDGL
Sbjct: 361 IHIKKANCSEWEEIHVPKSVRSIVVLNLYNYGSGRHPWGNLRPKYLEKRGFVEAHCDDGL 420

Query: 421 LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKNAFLQMDGEPWKQPMSDEF 480
           +E+FGLKQGWHAS VM E+ISAKHI QAAAIRFELRGG+WKNAFLQMDGEPWKQPM  ++
Sbjct: 421 IEIFGLKQGWHASFVMAEIISAKHIAQAAAIRFELRGGDWKNAFLQMDGEPWKQPMKSDY 480

Query: 481 STFVEIKRVPSQSLMINGE 491
           STFVEIK+VP QSLMINGE
Sbjct: 481 STFVEIKKVPFQSLMINGE 492

BLAST of MC08g2103 vs. ExPASy Swiss-Prot
Match: Q8VZG1 (Diacylglycerol kinase 3 OS=Arabidopsis thaliana OX=3702 GN=DGK3 PE=1 SV=1)

HSP 1 Score: 672.5 bits (1734), Expect = 3.5e-192
Identity = 318/495 (64.24%), Postives = 389/495 (78.59%), Query Frame = 0

Query: 1   MESLTTNGGEDSSTRIAARSSMIES--LKGCGLTG---IHVDKEELRKKLQMPQYLRFAM 60
           M+S  +         +A+R S  +S  ++GCGL     + VDK ELR++L MP+YLR AM
Sbjct: 1   MDSPVSKTDASKEKFVASRPSTADSKTMRGCGLANLAWVGVDKVELRQRLMMPEYLRLAM 60

Query: 61  RDSIRLQDPAAGESRLPAANSLSGSAFAPEINEPPESPMVVFINPRSGGRHGRLLKERLQ 120
           RD I+ +D +A    +P    L G A A   +  P +PMVVFINP SGGRHG +LKERLQ
Sbjct: 61  RDCIKRKDSSA----IPDHLLLPGGAVA---DMAPHAPMVVFINPNSGGRHGPVLKERLQ 120

Query: 121 MLISEEQVFDLTDVKPHEFVKYGLKCLELLAAIGDACAKETRDKMRVMVAGGDGTVGWVL 180
            L+SEEQVFDLT+VKPHEFV+YGL CLE +AA GD CAKE R ++R+MVAGGDGTVGWVL
Sbjct: 121 QLMSEEQVFDLTEVKPHEFVRYGLGCLEKVAAEGDECAKECRARLRIMVAGGDGTVGWVL 180

Query: 181 GCLLELEKQGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGRVCK 240
           GCL EL K G+  +PPVGVIPLGTGNDLSR+FGWGGSFPFAW+SA+KR+L RA+ G V +
Sbjct: 181 GCLGELNKDGKSQIPPVGVIPLGTGNDLSRSFGWGGSFPFAWRSAVKRTLHRASMGPVAR 240

Query: 241 LDSWNVLLSAPNSINVELPHCMRPTEDGALDESFEIKEASSEKASCHGGVFYNYFSIGMD 300
           LDSW +L+S P+   V+ P+ ++P E+  LD+  +    +   A  + GVFYNY SIGMD
Sbjct: 241 LDSWKILVSMPSGEVVDPPYSLKPAEENELDQGLDAGIDAPPLAKAYEGVFYNYLSIGMD 300

Query: 301 AQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVK 360
           AQVAYGFHHLRN KPYLAQGPI+NK+IYS + C QGWF TPC +DPGLRGL+NI+++H+K
Sbjct: 301 AQVAYGFHHLRNTKPYLAQGPISNKIIYSSFGCSQGWFCTPCVNDPGLRGLRNIMKIHIK 360

Query: 361 KVNCSDWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVF 420
           KVNCS WE++ +P +VRS+VALNL++YGSG HPWGNL P+Y+EKRGFVEA  DDGL+E+F
Sbjct: 361 KVNCSQWEEIAVPKNVRSIVALNLHSYGSGSHPWGNLKPDYLEKRGFVEAHCDDGLIEIF 420

Query: 421 GLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKNAFLQMDGEPWKQPMSDEFSTFV 480
           G KQGWHAS VM ELISAKHI QAAA+RFELRGG+W++AFLQMDGEPWKQPMS E+STFV
Sbjct: 421 GFKQGWHASFVMAELISAKHIAQAAAVRFELRGGDWRDAFLQMDGEPWKQPMSTEYSTFV 480

Query: 481 EIKRVPSQSLMINGE 491
           EIK+VP QSLMIN E
Sbjct: 481 EIKKVPYQSLMINNE 488

BLAST of MC08g2103 vs. ExPASy Swiss-Prot
Match: Q1PDI2 (Diacylglycerol kinase 4 OS=Arabidopsis thaliana OX=3702 GN=DGK4 PE=1 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 1.7e-183
Identity = 302/488 (61.89%), Postives = 382/488 (78.28%), Query Frame = 0

Query: 9   GEDSSTRIAARSSMIESLKG------CGLTGIHVDKEELRKKLQMPQYLRFAMRDSIRLQ 68
           G+  + R+  R S ++S           L  I V K ELR+++ +PQYLR A+RD I  +
Sbjct: 7   GDSLTARMIPRHSSLDSFGAMKVSLLVNLASIRVSKAELRQRVMLPQYLRIAIRDCILRK 66

Query: 69  DPAAGESRLPAANSLSGSAFAPEINEPPESPMVVFINPRSGGRHGRLLKERLQMLISEEQ 128
           D +   S    A  L  +A  PE+      P++VF+NP+SGGR G L+KERLQ LISEEQ
Sbjct: 67  DDSFDASS-SVAPPLENNALTPEV------PLMVFVNPKSGGRQGPLIKERLQNLISEEQ 126

Query: 129 VFDLTDVKPHEFVKYGLKCLELLAAIGDACAKETRDKMRVMVAGGDGTVGWVLGCLLELE 188
           V+DLT+VKP+EF++YGL CLE  A+ GD CAKE R+KMR++VAGGDGTVGWVLGCL EL 
Sbjct: 127 VYDLTEVKPNEFIRYGLGCLEAFASRGDECAKEIREKMRIVVAGGDGTVGWVLGCLGELN 186

Query: 189 KQGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGRVCKLDSWNVL 248
            Q RLPVPPV ++PLGTGNDLSR+FGWGGSFPFAWKSAIKR+L RA+   + +LDSWN+L
Sbjct: 187 LQNRLPVPPVSIMPLGTGNDLSRSFGWGGSFPFAWKSAIKRTLHRASVAPISRLDSWNIL 246

Query: 249 LSAPNSINVELPHCMRPTEDGALDESFEIKEASSEKASCHGGVFYNYFSIGMDAQVAYGF 308
           ++ P+   V+ P+ ++ T++  +D++ EI+       + + GVFYNYFSIGMDAQVAYGF
Sbjct: 247 ITMPSGEIVDPPYSLKATQECYIDQNLEIEGEIPPSTNGYEGVFYNYFSIGMDAQVAYGF 306

Query: 309 HHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVKKVNCSDW 368
           HHLRNEKPYLA GPIANK+IYSGY C QGWF T C +DPGLRGLKNI+ +H+KK++ S+W
Sbjct: 307 HHLRNEKPYLANGPIANKIIYSGYGCSQGWFLTHCINDPGLRGLKNIMTLHIKKLDSSEW 366

Query: 369 EQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGLKQGWH 428
           E+V +P SVR++VALNL++YGSGR+PWGNL  +Y+EKRGFVEAQ DDGLLE+FGLKQGWH
Sbjct: 367 EKVPVPKSVRAVVALNLHSYGSGRNPWGNLKQDYLEKRGFVEAQADDGLLEIFGLKQGWH 426

Query: 429 ASLVMGELISAKHIVQAAAIRFELRGGEWKNAFLQMDGEPWKQPMSDEFSTFVEIKRVPS 488
           AS VM ELISAKHI QAAAIR E+RGG+WK+AF+QMDGEPWKQPM+ ++STFV+IKRVP 
Sbjct: 427 ASFVMVELISAKHIAQAAAIRLEIRGGDWKDAFMQMDGEPWKQPMTRDYSTFVDIKRVPH 486

Query: 489 QSLMINGE 491
           QSL++ G+
Sbjct: 487 QSLVVKGD 487

BLAST of MC08g2103 vs. ExPASy Swiss-Prot
Match: Q9C5E5 (Diacylglycerol kinase 5 OS=Arabidopsis thaliana OX=3702 GN=DGK5 PE=2 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 2.2e-69
Identity = 144/414 (34.78%), Postives = 219/414 (52.90%), Query Frame = 0

Query: 85  EINEPPESPMVVFINPRSGGRHGRLLKERLQMLISEEQVFDLTDVKPHEFVKYGLKCLEL 144
           E    P SP++VFIN +SGG+ G  L    + L++  QVFDL    P + ++     LE 
Sbjct: 32  ESRPTPASPVLVFINSKSGGQLGGELILTYRSLLNHNQVFDLDQETPDKVLRRIYLNLER 91

Query: 145 LAAIGDACAKETRDKMRVMVAGGDGTVGWVLGCLLELEKQGRLPVPPVGVIPLGTGNDLS 204
           L    D  A++ R+K++++VAGGDGT GW+LG + +L+       PP+  +PLGTGN+L 
Sbjct: 92  LK--DDDFARQIREKLKIIVAGGDGTAGWLLGVVCDLKLS---HPPPIATVPLGTGNNLP 151

Query: 205 RTFGWGGSFPFAWKSAIKRSLDRATTGRVCKLDSWNVLL---------SAPNSINVELPH 264
             FGWG   P   ++A++  L++    +V K+D+W++L+         S      +ELPH
Sbjct: 152 FAFGWGKKNPGTDRTAVESFLEQVLKAKVMKIDNWHILMRMKTPKEGGSCDPVAPLELPH 211

Query: 265 CMRPTEDGALDESFEIKEASSEKASCHGGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQG 324
            +      A        E + E      G F+NYFS+GMDAQ++Y FH  R   P   + 
Sbjct: 212 SLH-----AFHRVSPTDELNKEGCHTFRGGFWNYFSLGMDAQISYAFHSERKLHPEKFKN 271

Query: 325 PIANKLIYSGYSCGQGWFFTPCSS--DPGLRGLKNILRMHVKKVNCSDWEQVLIPSSVRS 384
            + N+  Y    C QGWF   C+S   P  R +  + ++ +   N   W+ + IP S+RS
Sbjct: 272 QLVNQSTYVKLGCTQGWF---CASLFHPASRNIAQLAKVKIATRN-GQWQDLHIPHSIRS 331

Query: 385 LVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGLKQGWHASLVMGELISA 444
           +V LNL ++  G +PWG   P     RG     VDDGL+EV G +  WH  +++      
Sbjct: 332 IVCLNLPSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNAWHGLVLLAPNGHG 391

Query: 445 KHIVQAAAIRFELRGGEWKNAFLQMDGEPWKQPMS-DEFSTFVEIKRVPSQSLM 487
             + QA  IRFE   G   + F++MDGEPWKQP+  D+ +  VEI  +   +++
Sbjct: 392 TRLAQANRIRFEFHKGATDHTFMRMDGEPWKQPLPLDDETVMVEISHLGQVNML 431

BLAST of MC08g2103 vs. ExPASy Swiss-Prot
Match: F4JKI3 (Diacylglycerol kinase 6 OS=Arabidopsis thaliana OX=3702 GN=DGK6 PE=3 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 4.9e-69
Identity = 138/395 (34.94%), Postives = 217/395 (54.94%), Query Frame = 0

Query: 90  PESPMVVFINPRSGGRHGRLLKERLQMLISEEQVFDLTDVKPHEFVKYGLKCLELLAAIG 149
           PE+P++VFIN +SGG+ G  L    + L++++QVFDL    P + ++     LE L    
Sbjct: 44  PENPILVFINSKSGGQLGAELILTYRTLLNDKQVFDLEVETPDKVLQRIYLNLERLK--D 103

Query: 150 DACAKETRDKMRVMVAGGDGTVGWVLGCLLELEKQGRLPVPPVGVIPLGTGNDLSRTFGW 209
           D+ A + RDK++++VAGGDGT GW+LG + +L        PP+  +PLGTGN+L   FGW
Sbjct: 104 DSLASKIRDKLKIIVAGGDGTAGWLLGVVSDLNLSN---PPPIATVPLGTGNNLPFAFGW 163

Query: 210 GGSFPFAWKSAIKRSLDRATTGRVCKLDSWNVLLSAPN------SINVELPHCMRPTEDG 269
           G   P   +S+++  L +    +  K+D+W +L+   +       I ++LPH        
Sbjct: 164 GKKNPGTDRSSVESFLGKVINAKEMKIDNWKILMRMKHPKEGSCDITLKLPH-------- 223

Query: 270 ALDESFEIKEASSEKASCHGGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQGPIANKLIY 329
           +L   F   + + E    + G F+NYFS+GMDAQV+Y FH  R   P   +  + N+  Y
Sbjct: 224 SLPRIFPSDQENMEGYHTYRGGFWNYFSLGMDAQVSYAFHSQRKLHPERFKNQLVNQSTY 283

Query: 330 SGYSCGQGWFFTPCSSDPGLRGLKNILRMHVKKVNCSDWEQVLIPSSVRSLVALNLNNYG 389
              SC QGWFF      P  + +  + ++ +   N   W  + IP S+RS+V LNL ++ 
Sbjct: 284 LKLSCTQGWFFASL-FHPSSQNIAKLAKIQICDRN-GQWNDLHIPQSIRSIVCLNLPSFS 343

Query: 390 SGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGLKQGWHASLVMGELISAKHIVQAAAIR 449
            G +PWG   P+    R      VDDGL+E+ G +  WH  +++        + QA  +R
Sbjct: 344 GGLNPWGTPNPKKQRDRSLTAPFVDDGLIEIVGFRNAWHGLILLSPNGHGTRLAQANRVR 403

Query: 450 FELRGGEWKNAFLQMDGEPWKQPM-SDEFSTFVEI 478
            E + G  K+A++++DGEPWKQP+ S++ +  VEI
Sbjct: 404 LEFKKGAAKHAYMRIDGEPWKQPLPSNDETVMVEI 423

BLAST of MC08g2103 vs. NCBI nr
Match: XP_022153322.1 (diacylglycerol kinase 7-like [Momordica charantia])

HSP 1 Score: 1006 bits (2601), Expect = 0.0
Identity = 490/490 (100.00%), Postives = 490/490 (100.00%), Query Frame = 0

Query: 1   MESLTTNGGEDSSTRIAARSSMIESLKGCGLTGIHVDKEELRKKLQMPQYLRFAMRDSIR 60
           MESLTTNGGEDSSTRIAARSSMIESLKGCGLTGIHVDKEELRKKLQMPQYLRFAMRDSIR
Sbjct: 1   MESLTTNGGEDSSTRIAARSSMIESLKGCGLTGIHVDKEELRKKLQMPQYLRFAMRDSIR 60

Query: 61  LQDPAAGESRLPAANSLSGSAFAPEINEPPESPMVVFINPRSGGRHGRLLKERLQMLISE 120
           LQDPAAGESRLPAANSLSGSAFAPEINEPPESPMVVFINPRSGGRHGRLLKERLQMLISE
Sbjct: 61  LQDPAAGESRLPAANSLSGSAFAPEINEPPESPMVVFINPRSGGRHGRLLKERLQMLISE 120

Query: 121 EQVFDLTDVKPHEFVKYGLKCLELLAAIGDACAKETRDKMRVMVAGGDGTVGWVLGCLLE 180
           EQVFDLTDVKPHEFVKYGLKCLELLAAIGDACAKETRDKMRVMVAGGDGTVGWVLGCLLE
Sbjct: 121 EQVFDLTDVKPHEFVKYGLKCLELLAAIGDACAKETRDKMRVMVAGGDGTVGWVLGCLLE 180

Query: 181 LEKQGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGRVCKLDSWN 240
           LEKQGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGRVCKLDSWN
Sbjct: 181 LEKQGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGRVCKLDSWN 240

Query: 241 VLLSAPNSINVELPHCMRPTEDGALDESFEIKEASSEKASCHGGVFYNYFSIGMDAQVAY 300
           VLLSAPNSINVELPHCMRPTEDGALDESFEIKEASSEKASCHGGVFYNYFSIGMDAQVAY
Sbjct: 241 VLLSAPNSINVELPHCMRPTEDGALDESFEIKEASSEKASCHGGVFYNYFSIGMDAQVAY 300

Query: 301 GFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVKKVNCS 360
           GFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVKKVNCS
Sbjct: 301 GFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVKKVNCS 360

Query: 361 DWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGLKQG 420
           DWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGLKQG
Sbjct: 361 DWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGLKQG 420

Query: 421 WHASLVMGELISAKHIVQAAAIRFELRGGEWKNAFLQMDGEPWKQPMSDEFSTFVEIKRV 480
           WHASLVMGELISAKHIVQAAAIRFELRGGEWKNAFLQMDGEPWKQPMSDEFSTFVEIKRV
Sbjct: 421 WHASLVMGELISAKHIVQAAAIRFELRGGEWKNAFLQMDGEPWKQPMSDEFSTFVEIKRV 480

Query: 481 PSQSLMINGE 490
           PSQSLMINGE
Sbjct: 481 PSQSLMINGE 490

BLAST of MC08g2103 vs. NCBI nr
Match: XP_038890957.1 (diacylglycerol kinase 7-like [Benincasa hispida])

HSP 1 Score: 909 bits (2348), Expect = 0.0
Identity = 438/491 (89.21%), Postives = 466/491 (94.91%), Query Frame = 0

Query: 1   MESLTTNGGEDSSTRIAARSSMIESLKGCGLTGIHVDKEELRKKLQMPQYLRFAMRDSIR 60
           M S ++ G  DS+TRIAARSSMIES KGCGLTG+ +DKEELRKKL+MPQYLRFAMRDSIR
Sbjct: 1   MNSPSSRGEGDSTTRIAARSSMIESFKGCGLTGLRIDKEELRKKLRMPQYLRFAMRDSIR 60

Query: 61  LQDPAAGESRLPAANSLSGSAFAPEINE-PPESPMVVFINPRSGGRHGRLLKERLQMLIS 120
           LQDP+AGESRLP ANS+SGSAF PE ++ PP SPMVVFINPRSGGRHGRLLKERLQMLIS
Sbjct: 61  LQDPSAGESRLPGANSISGSAFVPENDDGPPASPMVVFINPRSGGRHGRLLKERLQMLIS 120

Query: 121 EEQVFDLTDVKPHEFVKYGLKCLELLAAIGDACAKETRDKMRVMVAGGDGTVGWVLGCLL 180
           EEQ+FDLTDVKPHEFVKYGL+CLELLA +GDACAK+ R+KMRVMVAGGDGTVGWVLGCLL
Sbjct: 121 EEQIFDLTDVKPHEFVKYGLRCLELLAGLGDACAKDVREKMRVMVAGGDGTVGWVLGCLL 180

Query: 181 ELEKQGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGRVCKLDSW 240
           ELEK+ RLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTG++ KLDSW
Sbjct: 181 ELEKKERLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGQIRKLDSW 240

Query: 241 NVLLSAPNSINVELPHCMRPTEDGALDESFEIKEASSEKASCHGGVFYNYFSIGMDAQVA 300
           +V+LS P+  NVELPHCM+PTE+ ALDES EI+EA SEKASCH GVFYNYFSIGMDAQVA
Sbjct: 241 HVILSTPSGENVELPHCMKPTEEHALDESLEIEEALSEKASCHEGVFYNYFSIGMDAQVA 300

Query: 301 YGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVKKVNC 360
           YGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDP LRGLKNILRMHVKKVNC
Sbjct: 301 YGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPRLRGLKNILRMHVKKVNC 360

Query: 361 SDWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGLKQ 420
           SDWEQ+LIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGLKQ
Sbjct: 361 SDWEQILIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGLKQ 420

Query: 421 GWHASLVMGELISAKHIVQAAAIRFELRGGEWKNAFLQMDGEPWKQPMSDEFSTFVEIKR 480
           GWHASLVMGELISAKHIVQAAAIRFELRGGEWK+AFLQMDGEPWKQPMSDEFSTFVEIK+
Sbjct: 421 GWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMSDEFSTFVEIKK 480

Query: 481 VPSQSLMINGE 490
           VPS SLMI+GE
Sbjct: 481 VPSHSLMISGE 491

BLAST of MC08g2103 vs. NCBI nr
Match: KAG6598863.1 (Diacylglycerol kinase 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 900 bits (2325), Expect = 0.0
Identity = 430/491 (87.58%), Postives = 464/491 (94.50%), Query Frame = 0

Query: 1   MESLTTNGGEDSSTRIAARSSMIESLKGCGLTGIHVDKEELRKKLQMPQYLRFAMRDSIR 60
           M+S T+ GG DS+TRI ARSSMIES KGCGLTG+ +DKE+LRK+L+MPQYLRFAMRDSIR
Sbjct: 1   MDSPTSRGGGDSTTRIPARSSMIESFKGCGLTGMRIDKEKLRKQLRMPQYLRFAMRDSIR 60

Query: 61  LQDPAAGESRLPAANSLSGSAFAPEINE-PPESPMVVFINPRSGGRHGRLLKERLQMLIS 120
           LQDP AGESRLP A+S+SGS FA + ++ PPESPMVVFINPRSGGRHGRLLKERLQMLIS
Sbjct: 61  LQDPGAGESRLPGASSISGSPFAQDTDDGPPESPMVVFINPRSGGRHGRLLKERLQMLIS 120

Query: 121 EEQVFDLTDVKPHEFVKYGLKCLELLAAIGDACAKETRDKMRVMVAGGDGTVGWVLGCLL 180
           EEQVFDLTDVKPHEFV+YGL+CLELLA +GDACAKETR+KMRVMVAGGDGTVGWVLGCLL
Sbjct: 121 EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKETREKMRVMVAGGDGTVGWVLGCLL 180

Query: 181 ELEKQGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGRVCKLDSW 240
           ELEK+GRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTG++ +LDSW
Sbjct: 181 ELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGKIRRLDSW 240

Query: 241 NVLLSAPNSINVELPHCMRPTEDGALDESFEIKEASSEKASCHGGVFYNYFSIGMDAQVA 300
            VLLS+P+  N++LPHCM+PTED ALDES EI+EA  EKA+CH GVFYNYFSIGMDAQVA
Sbjct: 241 QVLLSSPSGENMDLPHCMKPTEDHALDESLEIEEALPEKATCHEGVFYNYFSIGMDAQVA 300

Query: 301 YGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVKKVNC 360
           YGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDP LRGLKNILRMHVKK+NC
Sbjct: 301 YGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPSLRGLKNILRMHVKKINC 360

Query: 361 SDWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGLKQ 420
           SDWE + IPSSVRSLVALNLNNYGSGRHPWGNL+PEYMEKRGFVEA VDDGLLEVFGLKQ
Sbjct: 361 SDWEHISIPSSVRSLVALNLNNYGSGRHPWGNLSPEYMEKRGFVEAHVDDGLLEVFGLKQ 420

Query: 421 GWHASLVMGELISAKHIVQAAAIRFELRGGEWKNAFLQMDGEPWKQPMSDEFSTFVEIKR 480
           GWHASLVMGELISAKHIVQAAAIRFELRGGEW+NAFLQMDGEPWKQPM+DEFSTFVEIKR
Sbjct: 421 GWHASLVMGELISAKHIVQAAAIRFELRGGEWRNAFLQMDGEPWKQPMNDEFSTFVEIKR 480

Query: 481 VPSQSLMINGE 490
           VP QSLMI+GE
Sbjct: 481 VPCQSLMISGE 491

BLAST of MC08g2103 vs. NCBI nr
Match: XP_022929674.1 (diacylglycerol kinase 7-like [Cucurbita moschata] >KAG7029804.1 Diacylglycerol kinase 7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 898 bits (2321), Expect = 0.0
Identity = 429/491 (87.37%), Postives = 463/491 (94.30%), Query Frame = 0

Query: 1   MESLTTNGGEDSSTRIAARSSMIESLKGCGLTGIHVDKEELRKKLQMPQYLRFAMRDSIR 60
           M+S T+ GG DS+TRI ARSSMIES KGCGLTG+ +DKE+LRK+L+MPQYLRFAMRDSIR
Sbjct: 1   MDSPTSRGGGDSTTRIPARSSMIESFKGCGLTGMRIDKEKLRKQLRMPQYLRFAMRDSIR 60

Query: 61  LQDPAAGESRLPAANSLSGSAFAPEINE-PPESPMVVFINPRSGGRHGRLLKERLQMLIS 120
           LQDP AGESRLP A+S+SGS FA + ++ PPESPMVVFINPRSGGRHGRLLKERLQMLIS
Sbjct: 61  LQDPGAGESRLPGASSISGSPFAQDTDDGPPESPMVVFINPRSGGRHGRLLKERLQMLIS 120

Query: 121 EEQVFDLTDVKPHEFVKYGLKCLELLAAIGDACAKETRDKMRVMVAGGDGTVGWVLGCLL 180
           EEQVFDLTDVKPHEFV+YGL+CLELLA +GDACAKETR+KMRVMVAGGDGTVGWVLGCLL
Sbjct: 121 EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKETREKMRVMVAGGDGTVGWVLGCLL 180

Query: 181 ELEKQGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGRVCKLDSW 240
           ELEK+GRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTG++ +LDSW
Sbjct: 181 ELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGKIRRLDSW 240

Query: 241 NVLLSAPNSINVELPHCMRPTEDGALDESFEIKEASSEKASCHGGVFYNYFSIGMDAQVA 300
            V LS+P+  N++LPHCM+PTED ALDES EI+EA  EKA+CH GVFYNYFSIGMDAQVA
Sbjct: 241 QVFLSSPSGENMDLPHCMKPTEDHALDESLEIEEALPEKATCHEGVFYNYFSIGMDAQVA 300

Query: 301 YGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVKKVNC 360
           YGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDP LRGLKNILRMHVKK+NC
Sbjct: 301 YGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPSLRGLKNILRMHVKKINC 360

Query: 361 SDWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGLKQ 420
           SDWE + IPSSVRSLVALNLNNYGSGRHPWGNL+PEYMEKRGFVEA VDDGLLEVFGLKQ
Sbjct: 361 SDWEHISIPSSVRSLVALNLNNYGSGRHPWGNLSPEYMEKRGFVEAHVDDGLLEVFGLKQ 420

Query: 421 GWHASLVMGELISAKHIVQAAAIRFELRGGEWKNAFLQMDGEPWKQPMSDEFSTFVEIKR 480
           GWHASLVMGELISAKHIVQAAAIRFELRGGEW+NAFLQMDGEPWKQPM+DEFSTFVEIKR
Sbjct: 421 GWHASLVMGELISAKHIVQAAAIRFELRGGEWRNAFLQMDGEPWKQPMNDEFSTFVEIKR 480

Query: 481 VPSQSLMINGE 490
           VP QSLMI+GE
Sbjct: 481 VPCQSLMISGE 491

BLAST of MC08g2103 vs. NCBI nr
Match: XP_022997408.1 (diacylglycerol kinase 7-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 896 bits (2315), Expect = 0.0
Identity = 427/491 (86.97%), Postives = 463/491 (94.30%), Query Frame = 0

Query: 1   MESLTTNGGEDSSTRIAARSSMIESLKGCGLTGIHVDKEELRKKLQMPQYLRFAMRDSIR 60
           M+S T+ GG DS+TRI ARSSMIES KGCGLTG+ +DKE+LRK+L+MPQYLRFAMRDSIR
Sbjct: 1   MDSPTSRGGGDSTTRIPARSSMIESFKGCGLTGMRIDKEKLRKQLRMPQYLRFAMRDSIR 60

Query: 61  LQDPAAGESRLPAANSLSGSAFAPEINE-PPESPMVVFINPRSGGRHGRLLKERLQMLIS 120
           LQDP AGESRLP A+S+SGS FA + ++ PPESPMVVFINP+SGGRHGRLLKERLQMLIS
Sbjct: 61  LQDPGAGESRLPGASSISGSPFAQDTDDGPPESPMVVFINPQSGGRHGRLLKERLQMLIS 120

Query: 121 EEQVFDLTDVKPHEFVKYGLKCLELLAAIGDACAKETRDKMRVMVAGGDGTVGWVLGCLL 180
           EEQVFD+TDVKPHEFV+YGL+CLELLA +GDACAKETR+KMRVMVAGGDGTVGWVLGCLL
Sbjct: 121 EEQVFDITDVKPHEFVRYGLRCLELLAGLGDACAKETREKMRVMVAGGDGTVGWVLGCLL 180

Query: 181 ELEKQGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGRVCKLDSW 240
           ELEK+GRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTG++ +LDSW
Sbjct: 181 ELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGKIRRLDSW 240

Query: 241 NVLLSAPNSINVELPHCMRPTEDGALDESFEIKEASSEKASCHGGVFYNYFSIGMDAQVA 300
            V LS+P+  N++LPHCM+PTED ALDES EI+EA  EKA+CH GVFYNYFSIGMDAQVA
Sbjct: 241 QVFLSSPSGENMDLPHCMKPTEDHALDESLEIEEALPEKATCHEGVFYNYFSIGMDAQVA 300

Query: 301 YGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVKKVNC 360
           YGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDP LRGLKNILRMHVKK+NC
Sbjct: 301 YGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPSLRGLKNILRMHVKKINC 360

Query: 361 SDWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGLKQ 420
           SDWE + IPSSVRSLVALNLNNYGSGRHPWGNL+PEYMEKRGFVEA VDDGLLEVFGLKQ
Sbjct: 361 SDWEHISIPSSVRSLVALNLNNYGSGRHPWGNLSPEYMEKRGFVEAHVDDGLLEVFGLKQ 420

Query: 421 GWHASLVMGELISAKHIVQAAAIRFELRGGEWKNAFLQMDGEPWKQPMSDEFSTFVEIKR 480
           GWHASLVMGELISAKHIVQAAAIRFELRGGEW+NAFLQMDGEPWKQPM+DEFSTFVEIKR
Sbjct: 421 GWHASLVMGELISAKHIVQAAAIRFELRGGEWRNAFLQMDGEPWKQPMNDEFSTFVEIKR 480

Query: 481 VPSQSLMINGE 490
           VP QSLMI+GE
Sbjct: 481 VPCQSLMISGE 491

BLAST of MC08g2103 vs. ExPASy TrEMBL
Match: A0A6J1DIK2 (diacylglycerol kinase OS=Momordica charantia OX=3673 GN=LOC111020846 PE=3 SV=1)

HSP 1 Score: 1006 bits (2601), Expect = 0.0
Identity = 490/490 (100.00%), Postives = 490/490 (100.00%), Query Frame = 0

Query: 1   MESLTTNGGEDSSTRIAARSSMIESLKGCGLTGIHVDKEELRKKLQMPQYLRFAMRDSIR 60
           MESLTTNGGEDSSTRIAARSSMIESLKGCGLTGIHVDKEELRKKLQMPQYLRFAMRDSIR
Sbjct: 1   MESLTTNGGEDSSTRIAARSSMIESLKGCGLTGIHVDKEELRKKLQMPQYLRFAMRDSIR 60

Query: 61  LQDPAAGESRLPAANSLSGSAFAPEINEPPESPMVVFINPRSGGRHGRLLKERLQMLISE 120
           LQDPAAGESRLPAANSLSGSAFAPEINEPPESPMVVFINPRSGGRHGRLLKERLQMLISE
Sbjct: 61  LQDPAAGESRLPAANSLSGSAFAPEINEPPESPMVVFINPRSGGRHGRLLKERLQMLISE 120

Query: 121 EQVFDLTDVKPHEFVKYGLKCLELLAAIGDACAKETRDKMRVMVAGGDGTVGWVLGCLLE 180
           EQVFDLTDVKPHEFVKYGLKCLELLAAIGDACAKETRDKMRVMVAGGDGTVGWVLGCLLE
Sbjct: 121 EQVFDLTDVKPHEFVKYGLKCLELLAAIGDACAKETRDKMRVMVAGGDGTVGWVLGCLLE 180

Query: 181 LEKQGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGRVCKLDSWN 240
           LEKQGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGRVCKLDSWN
Sbjct: 181 LEKQGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGRVCKLDSWN 240

Query: 241 VLLSAPNSINVELPHCMRPTEDGALDESFEIKEASSEKASCHGGVFYNYFSIGMDAQVAY 300
           VLLSAPNSINVELPHCMRPTEDGALDESFEIKEASSEKASCHGGVFYNYFSIGMDAQVAY
Sbjct: 241 VLLSAPNSINVELPHCMRPTEDGALDESFEIKEASSEKASCHGGVFYNYFSIGMDAQVAY 300

Query: 301 GFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVKKVNCS 360
           GFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVKKVNCS
Sbjct: 301 GFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVKKVNCS 360

Query: 361 DWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGLKQG 420
           DWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGLKQG
Sbjct: 361 DWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGLKQG 420

Query: 421 WHASLVMGELISAKHIVQAAAIRFELRGGEWKNAFLQMDGEPWKQPMSDEFSTFVEIKRV 480
           WHASLVMGELISAKHIVQAAAIRFELRGGEWKNAFLQMDGEPWKQPMSDEFSTFVEIKRV
Sbjct: 421 WHASLVMGELISAKHIVQAAAIRFELRGGEWKNAFLQMDGEPWKQPMSDEFSTFVEIKRV 480

Query: 481 PSQSLMINGE 490
           PSQSLMINGE
Sbjct: 481 PSQSLMINGE 490

BLAST of MC08g2103 vs. ExPASy TrEMBL
Match: A0A6J1EPG0 (diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111436187 PE=3 SV=1)

HSP 1 Score: 898 bits (2321), Expect = 0.0
Identity = 429/491 (87.37%), Postives = 463/491 (94.30%), Query Frame = 0

Query: 1   MESLTTNGGEDSSTRIAARSSMIESLKGCGLTGIHVDKEELRKKLQMPQYLRFAMRDSIR 60
           M+S T+ GG DS+TRI ARSSMIES KGCGLTG+ +DKE+LRK+L+MPQYLRFAMRDSIR
Sbjct: 1   MDSPTSRGGGDSTTRIPARSSMIESFKGCGLTGMRIDKEKLRKQLRMPQYLRFAMRDSIR 60

Query: 61  LQDPAAGESRLPAANSLSGSAFAPEINE-PPESPMVVFINPRSGGRHGRLLKERLQMLIS 120
           LQDP AGESRLP A+S+SGS FA + ++ PPESPMVVFINPRSGGRHGRLLKERLQMLIS
Sbjct: 61  LQDPGAGESRLPGASSISGSPFAQDTDDGPPESPMVVFINPRSGGRHGRLLKERLQMLIS 120

Query: 121 EEQVFDLTDVKPHEFVKYGLKCLELLAAIGDACAKETRDKMRVMVAGGDGTVGWVLGCLL 180
           EEQVFDLTDVKPHEFV+YGL+CLELLA +GDACAKETR+KMRVMVAGGDGTVGWVLGCLL
Sbjct: 121 EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKETREKMRVMVAGGDGTVGWVLGCLL 180

Query: 181 ELEKQGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGRVCKLDSW 240
           ELEK+GRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTG++ +LDSW
Sbjct: 181 ELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGKIRRLDSW 240

Query: 241 NVLLSAPNSINVELPHCMRPTEDGALDESFEIKEASSEKASCHGGVFYNYFSIGMDAQVA 300
            V LS+P+  N++LPHCM+PTED ALDES EI+EA  EKA+CH GVFYNYFSIGMDAQVA
Sbjct: 241 QVFLSSPSGENMDLPHCMKPTEDHALDESLEIEEALPEKATCHEGVFYNYFSIGMDAQVA 300

Query: 301 YGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVKKVNC 360
           YGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDP LRGLKNILRMHVKK+NC
Sbjct: 301 YGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPSLRGLKNILRMHVKKINC 360

Query: 361 SDWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGLKQ 420
           SDWE + IPSSVRSLVALNLNNYGSGRHPWGNL+PEYMEKRGFVEA VDDGLLEVFGLKQ
Sbjct: 361 SDWEHISIPSSVRSLVALNLNNYGSGRHPWGNLSPEYMEKRGFVEAHVDDGLLEVFGLKQ 420

Query: 421 GWHASLVMGELISAKHIVQAAAIRFELRGGEWKNAFLQMDGEPWKQPMSDEFSTFVEIKR 480
           GWHASLVMGELISAKHIVQAAAIRFELRGGEW+NAFLQMDGEPWKQPM+DEFSTFVEIKR
Sbjct: 421 GWHASLVMGELISAKHIVQAAAIRFELRGGEWRNAFLQMDGEPWKQPMNDEFSTFVEIKR 480

Query: 481 VPSQSLMINGE 490
           VP QSLMI+GE
Sbjct: 481 VPCQSLMISGE 491

BLAST of MC08g2103 vs. ExPASy TrEMBL
Match: A0A6J1KDS8 (diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111492334 PE=3 SV=1)

HSP 1 Score: 896 bits (2315), Expect = 0.0
Identity = 427/491 (86.97%), Postives = 463/491 (94.30%), Query Frame = 0

Query: 1   MESLTTNGGEDSSTRIAARSSMIESLKGCGLTGIHVDKEELRKKLQMPQYLRFAMRDSIR 60
           M+S T+ GG DS+TRI ARSSMIES KGCGLTG+ +DKE+LRK+L+MPQYLRFAMRDSIR
Sbjct: 1   MDSPTSRGGGDSTTRIPARSSMIESFKGCGLTGMRIDKEKLRKQLRMPQYLRFAMRDSIR 60

Query: 61  LQDPAAGESRLPAANSLSGSAFAPEINE-PPESPMVVFINPRSGGRHGRLLKERLQMLIS 120
           LQDP AGESRLP A+S+SGS FA + ++ PPESPMVVFINP+SGGRHGRLLKERLQMLIS
Sbjct: 61  LQDPGAGESRLPGASSISGSPFAQDTDDGPPESPMVVFINPQSGGRHGRLLKERLQMLIS 120

Query: 121 EEQVFDLTDVKPHEFVKYGLKCLELLAAIGDACAKETRDKMRVMVAGGDGTVGWVLGCLL 180
           EEQVFD+TDVKPHEFV+YGL+CLELLA +GDACAKETR+KMRVMVAGGDGTVGWVLGCLL
Sbjct: 121 EEQVFDITDVKPHEFVRYGLRCLELLAGLGDACAKETREKMRVMVAGGDGTVGWVLGCLL 180

Query: 181 ELEKQGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGRVCKLDSW 240
           ELEK+GRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTG++ +LDSW
Sbjct: 181 ELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGKIRRLDSW 240

Query: 241 NVLLSAPNSINVELPHCMRPTEDGALDESFEIKEASSEKASCHGGVFYNYFSIGMDAQVA 300
            V LS+P+  N++LPHCM+PTED ALDES EI+EA  EKA+CH GVFYNYFSIGMDAQVA
Sbjct: 241 QVFLSSPSGENMDLPHCMKPTEDHALDESLEIEEALPEKATCHEGVFYNYFSIGMDAQVA 300

Query: 301 YGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVKKVNC 360
           YGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDP LRGLKNILRMHVKK+NC
Sbjct: 301 YGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPSLRGLKNILRMHVKKINC 360

Query: 361 SDWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGLKQ 420
           SDWE + IPSSVRSLVALNLNNYGSGRHPWGNL+PEYMEKRGFVEA VDDGLLEVFGLKQ
Sbjct: 361 SDWEHISIPSSVRSLVALNLNNYGSGRHPWGNLSPEYMEKRGFVEAHVDDGLLEVFGLKQ 420

Query: 421 GWHASLVMGELISAKHIVQAAAIRFELRGGEWKNAFLQMDGEPWKQPMSDEFSTFVEIKR 480
           GWHASLVMGELISAKHIVQAAAIRFELRGGEW+NAFLQMDGEPWKQPM+DEFSTFVEIKR
Sbjct: 421 GWHASLVMGELISAKHIVQAAAIRFELRGGEWRNAFLQMDGEPWKQPMNDEFSTFVEIKR 480

Query: 481 VPSQSLMINGE 490
           VP QSLMI+GE
Sbjct: 481 VPCQSLMISGE 491

BLAST of MC08g2103 vs. ExPASy TrEMBL
Match: A0A1S3BRW0 (diacylglycerol kinase OS=Cucumis melo OX=3656 GN=LOC103493013 PE=3 SV=1)

HSP 1 Score: 895 bits (2314), Expect = 0.0
Identity = 435/493 (88.24%), Postives = 462/493 (93.71%), Query Frame = 0

Query: 1   MESLTTNGGEDSSTRIAARSSMIESLKGCGLTGIHVDKEELRKKLQMPQYLRFAMRDSIR 60
           M+S T+ GG DS+TRIAARSSMIES KGCGLTG+ +DKE+LRKKLQMPQYLRFAMRDSIR
Sbjct: 1   MDSPTSRGGGDSTTRIAARSSMIESFKGCGLTGLRIDKEDLRKKLQMPQYLRFAMRDSIR 60

Query: 61  LQDPAAGESRLPAA--NSLSGSAFAPE-INEPPESPMVVFINPRSGGRHGRLLKERLQML 120
           LQDP+AGESRLP    +SLS S F PE   EPPESPMVVFINPRSGGRHGRLLK+RLQML
Sbjct: 61  LQDPSAGESRLPGTRLDSLSASTFVPESTEEPPESPMVVFINPRSGGRHGRLLKDRLQML 120

Query: 121 ISEEQVFDLTDVKPHEFVKYGLKCLELLAAIGDACAKETRDKMRVMVAGGDGTVGWVLGC 180
           ISEEQVFDLTDVKPHEFV+YGL CLELLA +GDACAK+ R K+RVMVAGGDGTVGWVLGC
Sbjct: 121 ISEEQVFDLTDVKPHEFVRYGLGCLELLAELGDACAKDVRQKIRVMVAGGDGTVGWVLGC 180

Query: 181 LLELEKQGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGRVCKLD 240
           LLELEK+ R PVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTG++ KLD
Sbjct: 181 LLELEKKDRRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGQIRKLD 240

Query: 241 SWNVLLSAPNSINVELPHCMRPTEDGALDESFEIKEASSEKASCHGGVFYNYFSIGMDAQ 300
           SW+V LS P+  NV+LPHCM+PTE+ ALDES EI+EA SEKASCH GVFYNYFSIGMDAQ
Sbjct: 241 SWHVFLSTPSGENVQLPHCMKPTEELALDESLEIEEALSEKASCHEGVFYNYFSIGMDAQ 300

Query: 301 VAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVKKV 360
           VAYGFH+LRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVKKV
Sbjct: 301 VAYGFHNLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVKKV 360

Query: 361 NCSDWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGL 420
           NCSDWEQ+LIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGL
Sbjct: 361 NCSDWEQILIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGL 420

Query: 421 KQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKNAFLQMDGEPWKQPMSDEFSTFVEI 480
           KQGWHASLVMGELISAKHIVQAAAIRFELRGGEWK+AFLQMDGEPWKQPMS+EFSTFVEI
Sbjct: 421 KQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMSNEFSTFVEI 480

Query: 481 KRVPSQSLMINGE 490
           KRVP QSLMI+GE
Sbjct: 481 KRVPCQSLMISGE 493

BLAST of MC08g2103 vs. ExPASy TrEMBL
Match: A0A0A0LK87 (diacylglycerol kinase OS=Cucumis sativus OX=3659 GN=Csa_2G058110 PE=3 SV=1)

HSP 1 Score: 884 bits (2283), Expect = 0.0
Identity = 429/493 (87.02%), Postives = 460/493 (93.31%), Query Frame = 0

Query: 1   MESLTTNGGEDSSTRIAARSSMIESLKGCGLTGIHVDKEELRKKLQMPQYLRFAMRDSIR 60
           M+S T+ GG+DS+TRIA+RSSMIES KGCGLTG+ +DKE+LRKKLQMPQYLRFAMRDSIR
Sbjct: 1   MDSPTSRGGDDSTTRIASRSSMIESFKGCGLTGLRIDKEDLRKKLQMPQYLRFAMRDSIR 60

Query: 61  LQDPAAGESRLPAA--NSLSGSAFAPEINE-PPESPMVVFINPRSGGRHGRLLKERLQML 120
           LQDP AGESRLP    +S+S S   PE  E PP+SPMVVFINPRSGGRHGRLLK+RLQML
Sbjct: 61  LQDPTAGESRLPGTRLDSISASTSVPETTEQPPDSPMVVFINPRSGGRHGRLLKDRLQML 120

Query: 121 ISEEQVFDLTDVKPHEFVKYGLKCLELLAAIGDACAKETRDKMRVMVAGGDGTVGWVLGC 180
           ISEEQVFDLTDVKPHEFV+YGL CLELLA +GDACAK+ R+KMRVMVAGGDGTVGWVLGC
Sbjct: 121 ISEEQVFDLTDVKPHEFVRYGLGCLELLAELGDACAKDVREKMRVMVAGGDGTVGWVLGC 180

Query: 181 LLELEKQGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGRVCKLD 240
           LLELEK+ R PVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTG++ KLD
Sbjct: 181 LLELEKKDRRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGQIRKLD 240

Query: 241 SWNVLLSAPNSINVELPHCMRPTEDGALDESFEIKEASSEKASCHGGVFYNYFSIGMDAQ 300
           SW+V LS P+  N +LPHCM+PTE+ ALDES EI+EA SEKASC+ GVFYNYFSIGMDAQ
Sbjct: 241 SWHVFLSTPSGENGKLPHCMKPTEELALDESLEIEEALSEKASCYEGVFYNYFSIGMDAQ 300

Query: 301 VAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVKKV 360
           VAYGFH+LRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVKKV
Sbjct: 301 VAYGFHNLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVKKV 360

Query: 361 NCSDWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGL 420
           NCSDWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGL
Sbjct: 361 NCSDWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGL 420

Query: 421 KQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKNAFLQMDGEPWKQPMSDEFSTFVEI 480
           KQGWHASLVMGELISAKHIVQAAAIRFELRGGEWK++FLQMDGEPWKQ MS+EFSTFVEI
Sbjct: 421 KQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDSFLQMDGEPWKQSMSNEFSTFVEI 480

Query: 481 KRVPSQSLMINGE 490
           KRVP QSLMI+GE
Sbjct: 481 KRVPCQSLMISGE 493

BLAST of MC08g2103 vs. TAIR 10
Match: AT4G30340.1 (diacylglycerol kinase 7 )

HSP 1 Score: 676.4 bits (1744), Expect = 1.7e-194
Identity = 323/499 (64.73%), Postives = 394/499 (78.96%), Query Frame = 0

Query: 1   MESLTTNGGEDSST-----RIAARSSMIESLKGCG---LTGIHVDKEELRKKLQMPQYLR 60
           ME    + GE S+T     R +A++    +++GCG   L  + +DKEELR +L MP+YLR
Sbjct: 1   MEETPRSVGEASTTNFVAARPSAKTDDAVTMRGCGFANLALVGIDKEELRGRLAMPEYLR 60

Query: 61  FAMRDSIRLQDPAAGESRLPAANSLSGSAFAPEINEPPESPMVVFINPRSGGRHGRLLKE 120
            AMRD I+ +D       L     L G A A   +  P +PMVVFINP+SGGRHG +LKE
Sbjct: 61  IAMRDCIKRKDSTEISDHL----LLPGGAAA---DMAPHAPMVVFINPKSGGRHGPVLKE 120

Query: 121 RLQMLISEEQVFDLTDVKPHEFVKYGLKCLELLAAIGDACAKETRDKMRVMVAGGDGTVG 180
           RLQ L++EEQVFDLT+VKPHEFV+YGL CL+ LAA GD CA+E R+K+R+MVAGGDGTVG
Sbjct: 121 RLQQLMTEEQVFDLTEVKPHEFVRYGLGCLDTLAAKGDECARECREKIRIMVAGGDGTVG 180

Query: 181 WVLGCLLELEKQGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGR 240
           WVLGCL EL K G+  +PPVGVIPLGTGNDLSR+F WGGSFPFAW+SA+KR+L RAT G 
Sbjct: 181 WVLGCLGELHKDGKSHIPPVGVIPLGTGNDLSRSFSWGGSFPFAWRSAMKRTLHRATLGS 240

Query: 241 VCKLDSWNVLLSAPNSINVELPHCMRPT-EDGALDESFEIKEASSEKASCHGGVFYNYFS 300
           + +LDSW +++S P+   V+ P+ ++PT E+ ALD++ +       KA  + GVFYNYFS
Sbjct: 241 IARLDSWKIVVSMPSGEVVDPPYSLKPTIEETALDQALDADGDVPPKAKSYEGVFYNYFS 300

Query: 301 IGMDAQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILR 360
           IGMDAQVAYGFHHLRNEKPYLAQGP+ NK+IYS YSC QGWF TPC ++P LRGL+NI++
Sbjct: 301 IGMDAQVAYGFHHLRNEKPYLAQGPVTNKIIYSSYSCTQGWFCTPCVNNPALRGLRNIMK 360

Query: 361 MHVKKVNCSDWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGL 420
           +H+KK NCS+WE++ +P SVRS+V LNL NYGSGRHPWGNL P+Y+EKRGFVEA  DDGL
Sbjct: 361 IHIKKANCSEWEEIHVPKSVRSIVVLNLYNYGSGRHPWGNLRPKYLEKRGFVEAHCDDGL 420

Query: 421 LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKNAFLQMDGEPWKQPMSDEF 480
           +E+FGLKQGWHAS VM E+ISAKHI QAAAIRFELRGG+WKNAFLQMDGEPWKQPM  ++
Sbjct: 421 IEIFGLKQGWHASFVMAEIISAKHIAQAAAIRFELRGGDWKNAFLQMDGEPWKQPMKSDY 480

Query: 481 STFVEIKRVPSQSLMINGE 491
           STFVEIK+VP QSLMINGE
Sbjct: 481 STFVEIKKVPFQSLMINGE 492

BLAST of MC08g2103 vs. TAIR 10
Match: AT2G18730.1 (diacylglycerol kinase 3 )

HSP 1 Score: 672.5 bits (1734), Expect = 2.5e-193
Identity = 318/495 (64.24%), Postives = 389/495 (78.59%), Query Frame = 0

Query: 1   MESLTTNGGEDSSTRIAARSSMIES--LKGCGLTG---IHVDKEELRKKLQMPQYLRFAM 60
           M+S  +         +A+R S  +S  ++GCGL     + VDK ELR++L MP+YLR AM
Sbjct: 1   MDSPVSKTDASKEKFVASRPSTADSKTMRGCGLANLAWVGVDKVELRQRLMMPEYLRLAM 60

Query: 61  RDSIRLQDPAAGESRLPAANSLSGSAFAPEINEPPESPMVVFINPRSGGRHGRLLKERLQ 120
           RD I+ +D +A    +P    L G A A   +  P +PMVVFINP SGGRHG +LKERLQ
Sbjct: 61  RDCIKRKDSSA----IPDHLLLPGGAVA---DMAPHAPMVVFINPNSGGRHGPVLKERLQ 120

Query: 121 MLISEEQVFDLTDVKPHEFVKYGLKCLELLAAIGDACAKETRDKMRVMVAGGDGTVGWVL 180
            L+SEEQVFDLT+VKPHEFV+YGL CLE +AA GD CAKE R ++R+MVAGGDGTVGWVL
Sbjct: 121 QLMSEEQVFDLTEVKPHEFVRYGLGCLEKVAAEGDECAKECRARLRIMVAGGDGTVGWVL 180

Query: 181 GCLLELEKQGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGRVCK 240
           GCL EL K G+  +PPVGVIPLGTGNDLSR+FGWGGSFPFAW+SA+KR+L RA+ G V +
Sbjct: 181 GCLGELNKDGKSQIPPVGVIPLGTGNDLSRSFGWGGSFPFAWRSAVKRTLHRASMGPVAR 240

Query: 241 LDSWNVLLSAPNSINVELPHCMRPTEDGALDESFEIKEASSEKASCHGGVFYNYFSIGMD 300
           LDSW +L+S P+   V+ P+ ++P E+  LD+  +    +   A  + GVFYNY SIGMD
Sbjct: 241 LDSWKILVSMPSGEVVDPPYSLKPAEENELDQGLDAGIDAPPLAKAYEGVFYNYLSIGMD 300

Query: 301 AQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVK 360
           AQVAYGFHHLRN KPYLAQGPI+NK+IYS + C QGWF TPC +DPGLRGL+NI+++H+K
Sbjct: 301 AQVAYGFHHLRNTKPYLAQGPISNKIIYSSFGCSQGWFCTPCVNDPGLRGLRNIMKIHIK 360

Query: 361 KVNCSDWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVF 420
           KVNCS WE++ +P +VRS+VALNL++YGSG HPWGNL P+Y+EKRGFVEA  DDGL+E+F
Sbjct: 361 KVNCSQWEEIAVPKNVRSIVALNLHSYGSGSHPWGNLKPDYLEKRGFVEAHCDDGLIEIF 420

Query: 421 GLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKNAFLQMDGEPWKQPMSDEFSTFV 480
           G KQGWHAS VM ELISAKHI QAAA+RFELRGG+W++AFLQMDGEPWKQPMS E+STFV
Sbjct: 421 GFKQGWHASFVMAELISAKHIAQAAAVRFELRGGDWRDAFLQMDGEPWKQPMSTEYSTFV 480

Query: 481 EIKRVPSQSLMINGE 491
           EIK+VP QSLMIN E
Sbjct: 481 EIKKVPYQSLMINNE 488

BLAST of MC08g2103 vs. TAIR 10
Match: AT5G57690.1 (diacylglycerol kinase 4 )

HSP 1 Score: 643.7 bits (1659), Expect = 1.2e-184
Identity = 302/488 (61.89%), Postives = 382/488 (78.28%), Query Frame = 0

Query: 9   GEDSSTRIAARSSMIESLKG------CGLTGIHVDKEELRKKLQMPQYLRFAMRDSIRLQ 68
           G+  + R+  R S ++S           L  I V K ELR+++ +PQYLR A+RD I  +
Sbjct: 7   GDSLTARMIPRHSSLDSFGAMKVSLLVNLASIRVSKAELRQRVMLPQYLRIAIRDCILRK 66

Query: 69  DPAAGESRLPAANSLSGSAFAPEINEPPESPMVVFINPRSGGRHGRLLKERLQMLISEEQ 128
           D +   S    A  L  +A  PE+      P++VF+NP+SGGR G L+KERLQ LISEEQ
Sbjct: 67  DDSFDASS-SVAPPLENNALTPEV------PLMVFVNPKSGGRQGPLIKERLQNLISEEQ 126

Query: 129 VFDLTDVKPHEFVKYGLKCLELLAAIGDACAKETRDKMRVMVAGGDGTVGWVLGCLLELE 188
           V+DLT+VKP+EF++YGL CLE  A+ GD CAKE R+KMR++VAGGDGTVGWVLGCL EL 
Sbjct: 127 VYDLTEVKPNEFIRYGLGCLEAFASRGDECAKEIREKMRIVVAGGDGTVGWVLGCLGELN 186

Query: 189 KQGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGRVCKLDSWNVL 248
            Q RLPVPPV ++PLGTGNDLSR+FGWGGSFPFAWKSAIKR+L RA+   + +LDSWN+L
Sbjct: 187 LQNRLPVPPVSIMPLGTGNDLSRSFGWGGSFPFAWKSAIKRTLHRASVAPISRLDSWNIL 246

Query: 249 LSAPNSINVELPHCMRPTEDGALDESFEIKEASSEKASCHGGVFYNYFSIGMDAQVAYGF 308
           ++ P+   V+ P+ ++ T++  +D++ EI+       + + GVFYNYFSIGMDAQVAYGF
Sbjct: 247 ITMPSGEIVDPPYSLKATQECYIDQNLEIEGEIPPSTNGYEGVFYNYFSIGMDAQVAYGF 306

Query: 309 HHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVKKVNCSDW 368
           HHLRNEKPYLA GPIANK+IYSGY C QGWF T C +DPGLRGLKNI+ +H+KK++ S+W
Sbjct: 307 HHLRNEKPYLANGPIANKIIYSGYGCSQGWFLTHCINDPGLRGLKNIMTLHIKKLDSSEW 366

Query: 369 EQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGLKQGWH 428
           E+V +P SVR++VALNL++YGSGR+PWGNL  +Y+EKRGFVEAQ DDGLLE+FGLKQGWH
Sbjct: 367 EKVPVPKSVRAVVALNLHSYGSGRNPWGNLKQDYLEKRGFVEAQADDGLLEIFGLKQGWH 426

Query: 429 ASLVMGELISAKHIVQAAAIRFELRGGEWKNAFLQMDGEPWKQPMSDEFSTFVEIKRVPS 488
           AS VM ELISAKHI QAAAIR E+RGG+WK+AF+QMDGEPWKQPM+ ++STFV+IKRVP 
Sbjct: 427 ASFVMVELISAKHIAQAAAIRLEIRGGDWKDAFMQMDGEPWKQPMTRDYSTFVDIKRVPH 486

Query: 489 QSLMINGE 491
           QSL++ G+
Sbjct: 487 QSLVVKGD 487

BLAST of MC08g2103 vs. TAIR 10
Match: AT2G20900.1 (diacylglycerol kinase 5 )

HSP 1 Score: 264.6 bits (675), Expect = 1.6e-70
Identity = 144/414 (34.78%), Postives = 219/414 (52.90%), Query Frame = 0

Query: 85  EINEPPESPMVVFINPRSGGRHGRLLKERLQMLISEEQVFDLTDVKPHEFVKYGLKCLEL 144
           E    P SP++VFIN +SGG+ G  L    + L++  QVFDL    P + ++     LE 
Sbjct: 32  ESRPTPASPVLVFINSKSGGQLGGELILTYRSLLNHNQVFDLDQETPDKVLRRIYLNLER 91

Query: 145 LAAIGDACAKETRDKMRVMVAGGDGTVGWVLGCLLELEKQGRLPVPPVGVIPLGTGNDLS 204
           L    D  A++ R+K++++VAGGDGT GW+LG + +L+       PP+  +PLGTGN+L 
Sbjct: 92  LK--DDDFARQIREKLKIIVAGGDGTAGWLLGVVCDLKLS---HPPPIATVPLGTGNNLP 151

Query: 205 RTFGWGGSFPFAWKSAIKRSLDRATTGRVCKLDSWNVLL---------SAPNSINVELPH 264
             FGWG   P   ++A++  L++    +V K+D+W++L+         S      +ELPH
Sbjct: 152 FAFGWGKKNPGTDRTAVESFLEQVLKAKVMKIDNWHILMRMKTPKEGGSCDPVAPLELPH 211

Query: 265 CMRPTEDGALDESFEIKEASSEKASCHGGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQG 324
            +      A        E + E      G F+NYFS+GMDAQ++Y FH  R   P   + 
Sbjct: 212 SLH-----AFHRVSPTDELNKEGCHTFRGGFWNYFSLGMDAQISYAFHSERKLHPEKFKN 271

Query: 325 PIANKLIYSGYSCGQGWFFTPCSS--DPGLRGLKNILRMHVKKVNCSDWEQVLIPSSVRS 384
            + N+  Y    C QGWF   C+S   P  R +  + ++ +   N   W+ + IP S+RS
Sbjct: 272 QLVNQSTYVKLGCTQGWF---CASLFHPASRNIAQLAKVKIATRN-GQWQDLHIPHSIRS 331

Query: 385 LVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGLKQGWHASLVMGELISA 444
           +V LNL ++  G +PWG   P     RG     VDDGL+EV G +  WH  +++      
Sbjct: 332 IVCLNLPSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNAWHGLVLLAPNGHG 391

Query: 445 KHIVQAAAIRFELRGGEWKNAFLQMDGEPWKQPMS-DEFSTFVEIKRVPSQSLM 487
             + QA  IRFE   G   + F++MDGEPWKQP+  D+ +  VEI  +   +++
Sbjct: 392 TRLAQANRIRFEFHKGATDHTFMRMDGEPWKQPLPLDDETVMVEISHLGQVNML 431

BLAST of MC08g2103 vs. TAIR 10
Match: AT2G20900.3 (diacylglycerol kinase 5 )

HSP 1 Score: 264.6 bits (675), Expect = 1.6e-70
Identity = 144/414 (34.78%), Postives = 219/414 (52.90%), Query Frame = 0

Query: 85  EINEPPESPMVVFINPRSGGRHGRLLKERLQMLISEEQVFDLTDVKPHEFVKYGLKCLEL 144
           E    P SP++VFIN +SGG+ G  L    + L++  QVFDL    P + ++     LE 
Sbjct: 32  ESRPTPASPVLVFINSKSGGQLGGELILTYRSLLNHNQVFDLDQETPDKVLRRIYLNLER 91

Query: 145 LAAIGDACAKETRDKMRVMVAGGDGTVGWVLGCLLELEKQGRLPVPPVGVIPLGTGNDLS 204
           L    D  A++ R+K++++VAGGDGT GW+LG + +L+       PP+  +PLGTGN+L 
Sbjct: 92  LK--DDDFARQIREKLKIIVAGGDGTAGWLLGVVCDLKLS---HPPPIATVPLGTGNNLP 151

Query: 205 RTFGWGGSFPFAWKSAIKRSLDRATTGRVCKLDSWNVLL---------SAPNSINVELPH 264
             FGWG   P   ++A++  L++    +V K+D+W++L+         S      +ELPH
Sbjct: 152 FAFGWGKKNPGTDRTAVESFLEQVLKAKVMKIDNWHILMRMKTPKEGGSCDPVAPLELPH 211

Query: 265 CMRPTEDGALDESFEIKEASSEKASCHGGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQG 324
            +      A        E + E      G F+NYFS+GMDAQ++Y FH  R   P   + 
Sbjct: 212 SLH-----AFHRVSPTDELNKEGCHTFRGGFWNYFSLGMDAQISYAFHSERKLHPEKFKN 271

Query: 325 PIANKLIYSGYSCGQGWFFTPCSS--DPGLRGLKNILRMHVKKVNCSDWEQVLIPSSVRS 384
            + N+  Y    C QGWF   C+S   P  R +  + ++ +   N   W+ + IP S+RS
Sbjct: 272 QLVNQSTYVKLGCTQGWF---CASLFHPASRNIAQLAKVKIATRN-GQWQDLHIPHSIRS 331

Query: 385 LVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGLKQGWHASLVMGELISA 444
           +V LNL ++  G +PWG   P     RG     VDDGL+EV G +  WH  +++      
Sbjct: 332 IVCLNLPSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNAWHGLVLLAPNGHG 391

Query: 445 KHIVQAAAIRFELRGGEWKNAFLQMDGEPWKQPMS-DEFSTFVEIKRVPSQSLM 487
             + QA  IRFE   G   + F++MDGEPWKQP+  D+ +  VEI  +   +++
Sbjct: 392 TRLAQANRIRFEFHKGATDHTFMRMDGEPWKQPLPLDDETVMVEISHLGQVNML 431