MC08g2075 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g2075 vs. ExPASy Swiss-Prot
Match: O64481 (Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana OX=3702 GN=SBT2.5 PE=2 SV=1)

HSP 1 Score: 1344.3 bits (3478), Expect = 0.0e+00
Identity = 659/809 (81.46%), Postives = 728/809 (89.99%), Query Frame = 0

Query: 9   VLVFLALLVVGRADIYIVSIEGEPIVSYRGDLDGFEATAVESDEKIDPTSELVTSYARHL 68
           + V   LLV   A++YIV++EG+PI+SY+G  +GFEATAVESDEKID +SELVT YARHL
Sbjct: 7   IFVVFVLLVAVTAEVYIVTMEGDPIISYKGGENGFEATAVESDEKIDTSSELVTVYARHL 66

Query: 69  ENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAEILRHTPIVKSVERDWKVRRL 128
           E KHDM+LGMLFE GS+KKLYSYKHLINGFA  ++PEQAE LR  P V+SV++DWKVRRL
Sbjct: 67  ERKHDMILGMLFEEGSYKKLYSYKHLINGFAAHVSPEQAETLRRAPGVRSVDKDWKVRRL 126

Query: 129 TTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPFGPCLKYKG 188
           TTHTPEFLGLPT VWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFA+++  P+GP   YKG
Sbjct: 127 TTHTPEFLGLPTDVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFASHHRLPYGPLPHYKG 186

Query: 189 KCEVDPNTKKSFCNGKIIGAQHFAEAAKAAGAFNPSIHFASPLDGDGHGSHTAAIAAGNN 248
           KCE DP+TKKSFCN KI+GAQHFAEAAKAAGAFNP I +ASP+DGDGHGSHTAAIAAGNN
Sbjct: 187 KCEEDPHTKKSFCNRKIVGAQHFAEAAKAAGAFNPDIDYASPMDGDGHGSHTAAIAAGNN 246

Query: 249 GIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSVG 308
           GIP+RMHGYEFGKASGMAPRARIAVYKALYR+FGGFVADVVAAIDQAVHDGVDILSLSVG
Sbjct: 247 GIPLRMHGYEFGKASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSVG 306

Query: 309 PNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAIDD 368
           PNSPP TTK T+LNPFDATLL AVKAGVFVAQAAGNGGPFPKTLVSYSPWI TVAAAIDD
Sbjct: 307 PNSPPTTTKTTFLNPFDATLLGAVKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAIDD 366

Query: 369 RRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLIDSSVTKYSPSDCQRPEVLNK 428
           RRYKNHLTLGNGK+LAG+GLSP T  +R YTLV+ANDVL+DSSV+KY+PSDCQRPEV NK
Sbjct: 367 RRYKNHLTLGNGKMLAGMGLSPPTRPHRLYTLVSANDVLLDSSVSKYNPSDCQRPEVFNK 426

Query: 429 RLVEGKVLLCGYSFSFVVGTASVKKVSQTAKALGAAGFVLAVENVSPGAKFDPVPVGIPG 488
           +LVEG +LLCGYSF+FVVGTAS+KKV  TAK LGAAGFVL VENVSPG KFDPVP  IPG
Sbjct: 427 KLVEGNILLCGYSFNFVVGTASIKKVVATAKHLGAAGFVLVVENVSPGTKFDPVPSAIPG 486

Query: 489 ILITDVSKSMDLIDYYNTSTPRDWTGRVKSFEAVGRIGDGLMPLLYKSAPEVALFSARGP 548
           ILITDVSKSMDLIDYYN ST RDWTGRVKSF+A G IGDGL P+L+KSAP+VALFSARGP
Sbjct: 487 ILITDVSKSMDLIDYYNASTSRDWTGRVKSFKAEGSIGDGLAPVLHKSAPQVALFSARGP 546

Query: 549 NIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIAGIA 608
           N +DFSFQDADLLKPDILAPG LIWAAW PNGTDEPNYVGEGFA+ISGTSMAAPHIAGIA
Sbjct: 547 NTKDFSFQDADLLKPDILAPGYLIWAAWCPNGTDEPNYVGEGFALISGTSMAAPHIAGIA 606

Query: 609 ALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQCSETEAMKLVPATPFDYGSGAVN 668
           ALVKQKHP WSPAAIKSALMTTST +DRAGR L+AQQ S+TEA+ LV ATPFDYGSG VN
Sbjct: 607 ALVKQKHPQWSPAAIKSALMTTSTVIDRAGRLLQAQQYSDTEAVTLVKATPFDYGSGHVN 666

Query: 669 PRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTSSPCNFTMGHPWNLNSPSITISH 728
           P AALDPGLIFDAGYEDYLGFLCTT GI+ HEI NYT++ CN+ M HP N N+PSI +SH
Sbjct: 667 PSAALDPGLIFDAGYEDYLGFLCTTPGISAHEIRNYTNTACNYDMKHPSNFNAPSIAVSH 726

Query: 729 LVGTQTVTRTVTNVAE-EETYVLTARMDPAVAIETDPPAMTLRSGSSRKFTVTLTARSLT 788
           LVGTQTVTR VTNVAE EETY +TARM P++AIE +PPAMTLR G++R F+VT+T RS++
Sbjct: 727 LVGTQTVTRKVTNVAEVEETYTITARMQPSIAIEVNPPAMTLRPGATRTFSVTMTVRSVS 786

Query: 789 GTYSFGEVLMKGSRGHKVRIPVVAMGYQR 817
           G YSFGEV +KGSRGHKVRIPVVA+G++R
Sbjct: 787 GVYSFGEVKLKGSRGHKVRIPVVALGHRR 815

BLAST of MC08g2075 vs. ExPASy Swiss-Prot
Match: Q9SZV5 (Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana OX=3702 GN=SBT2.6 PE=2 SV=1)

HSP 1 Score: 1331.2 bits (3444), Expect = 0.0e+00
Identity = 655/810 (80.86%), Postives = 728/810 (89.88%), Query Frame = 0

Query: 9   VLVFLALLVVGRADIYIVSIEGEPIVSYRGDLDGFEATAVESDEKIDPTSELVTSYARHL 68
           VLVF    +   A+IYIV++EGEPI+SY+G  +GFEATAVESDEKID TSELVTSYARHL
Sbjct: 7   VLVFFTCFLTVTAEIYIVTMEGEPIISYKGGDNGFEATAVESDEKIDTTSELVTSYARHL 66

Query: 69  ENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAEILRHTPIVKSVERDWKVRRL 128
           E KHDMLLGMLF  GS+KKLYSYKHLINGFA  ++P+QAE+LR  P VKSV+RDWKVR+L
Sbjct: 67  ERKHDMLLGMLFVEGSYKKLYSYKHLINGFAAHVSPDQAEMLRRAPGVKSVDRDWKVRKL 126

Query: 129 TTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTE-PFGPCLKYK 188
           TTHTP+FLGLPT VWPTGGG+DRAGEDIVIGF+DSGI+PHHPSFA+++T  P+GP   YK
Sbjct: 127 TTHTPQFLGLPTDVWPTGGGYDRAGEDIVIGFIDSGIFPHHPSFASHHTTVPYGPHPSYK 186

Query: 189 GKCEVDPNTKKSFCNGKIIGAQHFAEAAKAAGAFNPSIHFASPLDGDGHGSHTAAIAAGN 248
           GKCE DP+TK SFCNGKIIGAQHFAEAAKAAGAFNP I FASP+DGDGHGSHTAAIAAGN
Sbjct: 187 GKCEEDPHTKISFCNGKIIGAQHFAEAAKAAGAFNPDIDFASPMDGDGHGSHTAAIAAGN 246

Query: 249 NGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSV 308
           NGIPVRMHGYEFGKASGMAPRARIAVYKALYR+FGGFVADVVAAIDQAVHDGVDILSLSV
Sbjct: 247 NGIPVRMHGYEFGKASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSV 306

Query: 309 GPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAID 368
           GPNSPPATTK T+LNPFDATLL AVKAGVFVAQAAGNGGPFPKTLVSYSPWI TVAAAID
Sbjct: 307 GPNSPPATTKTTFLNPFDATLLGAVKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAID 366

Query: 369 DRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLIDSSVTKYSPSDCQRPEVLN 428
           DRRYKNHLTLGNGK+LAG+GLSP+T  +R+Y +V+ANDVL+ SS  KY+PSDCQ+PEVLN
Sbjct: 367 DRRYKNHLTLGNGKMLAGIGLSPSTRPHRSYKMVSANDVLLGSSGMKYNPSDCQKPEVLN 426

Query: 429 KRLVEGKVLLCGYSFSFVVGTASVKKVSQTAKALGAAGFVLAVENVSPGAKFDPVPVGIP 488
           K+LVEG +LLCGYSF+FV G+AS+KKV++TAK LGAAGFVL VENVSPG KFDPVP  IP
Sbjct: 427 KKLVEGNILLCGYSFNFVAGSASIKKVAETAKHLGAAGFVLVVENVSPGTKFDPVPSCIP 486

Query: 489 GILITDVSKSMDLIDYYNTSTPRDWTGRVKSFEAVGRIGDGLMPLLYKSAPEVALFSARG 548
           GILITDVSKSMDLIDYYN +T RDW GRVK F+A G IGDGL P+L+KSAPEVALFSARG
Sbjct: 487 GILITDVSKSMDLIDYYNVTTSRDWMGRVKDFKAEGSIGDGLEPILHKSAPEVALFSARG 546

Query: 549 PNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIAGI 608
           PN +DFSFQDADLLKPDILAPGSLIW+AWS NGTDE NY+GEGFA+ISGTSMAAPHIAGI
Sbjct: 547 PNTKDFSFQDADLLKPDILAPGSLIWSAWSANGTDEANYIGEGFALISGTSMAAPHIAGI 606

Query: 609 AALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQCSETEAMKLVPATPFDYGSGAV 668
           AALVKQKHP WSPAAIKSALMTTST +DRAGRPL+AQQ SETE + LV ATPFDYGSG V
Sbjct: 607 AALVKQKHPQWSPAAIKSALMTTSTVIDRAGRPLQAQQYSETETVTLVKATPFDYGSGHV 666

Query: 669 NPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTSSPCNFTMGHPWNLNSPSITIS 728
           NP AALDPGLIFDAGYEDY+GFLCTT GI+ HEI N+T++PCNF M HP N N+PSI IS
Sbjct: 667 NPSAALDPGLIFDAGYEDYIGFLCTTPGIDAHEIKNFTNTPCNFKMVHPSNFNTPSIAIS 726

Query: 729 HLVGTQTVTRTVTNVA-EEETYVLTARMDPAVAIETDPPAMTLRSGSSRKFTVTLTARSL 788
           HLV TQTVTR VTNVA EEETY +T+RM+PA+AIE  PPAMT+R+G+SR F+VTLT RS+
Sbjct: 727 HLVRTQTVTRRVTNVAEEEETYTITSRMEPAIAIEVSPPAMTVRAGASRTFSVTLTVRSV 786

Query: 789 TGTYSFGEVLMKGSRGHKVRIPVVAMGYQR 817
           TG YSFG+V +KGSRGHKV +PVVAMG +R
Sbjct: 787 TGAYSFGQVTLKGSRGHKVTLPVVAMGQRR 816

BLAST of MC08g2075 vs. ExPASy Swiss-Prot
Match: Q9FI12 (Subtilisin-like protease SBT2.3 OS=Arabidopsis thaliana OX=3702 GN=SBT2.3 PE=2 SV=1)

HSP 1 Score: 653.3 bits (1684), Expect = 3.7e-186
Identity = 368/805 (45.71%), Postives = 499/805 (61.99%), Query Frame = 0

Query: 23  IYIVSIEGEPIVSYRGDLDGFEATAVESDEKIDPTSELVTSYARHLENK-------HDML 82
           +YIV+++  PIV    + +  +    +   K+ P +    S  RH ++K       HD  
Sbjct: 38  VYIVTLKQPPIVHLFEEQE-LKHKKSKFTPKLRPRN---NSRKRHGKSKIPSVVQSHDSF 97

Query: 83  LGMLFERGSFKKLYSYKHLINGFAVDITPEQAEILRHTPIVKSVERDWKVRRLTTHTPEF 142
           L    +   + KLYSY +LINGFA+ I  +QAE L     V ++  D+ VR  TT+TP+F
Sbjct: 98  LRKTLKGEKYIKLYSYHYLINGFALFINSQQAEKLSMRKEVANIVLDYSVRTATTYTPQF 157

Query: 143 LGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPFGPCLK-YKGKCEVDP 202
           +GLP G W   GGF+ AGE ++IGF+D+GI P+HPSF   +++   P  K + G CEV P
Sbjct: 158 MGLPQGAWVKEGGFEIAGEGVIIGFIDTGIDPNHPSFNDNDSKRSYPIPKHFSGVCEVTP 217

Query: 203 NTKKSFCNGKIIGAQHFAEAAKAAGAFNPSIHFASPLDGDGHGSHTAAIAAGNNGIPVRM 262
           +     CN K+IGA+HFA++A   G FN S  +ASP DGDGHG+HTA++AAGN+G+PV +
Sbjct: 218 DFPSGSCNKKLIGARHFAQSAVTRGIFNSSEDYASPFDGDGHGTHTASVAAGNHGVPVIV 277

Query: 263 HGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPPA 322
             + FG ASG+APRA I+VYKALY+ FGGF ADVVAAIDQA  DGVDILSLS+ PN  P 
Sbjct: 278 SNHNFGYASGIAPRAFISVYKALYKSFGGFAADVVAAIDQAAQDGVDILSLSITPNRKPP 337

Query: 323 TTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAIDDRRYKNH 382
               T+ NP D  LLSAVKAG+FV QAAGN GP PKT+ S+SPWI TV A+  DR Y N 
Sbjct: 338 GV-ATFFNPIDMALLSAVKAGIFVVQAAGNTGPAPKTMSSFSPWIFTVGASSHDRVYSNS 397

Query: 383 LTLGNGKILAGLGLSPATHLNRTYTLVAANDVLIDS-SVTK-YSPSDCQRPEVLNKRLVE 442
           LTLGN   + G+G +  T   + Y +++A   L +S SV K     +CQ  E  ++  V 
Sbjct: 398 LTLGNNVTIPGMGFAIPTDSGKMYKMISAFHALNNSTSVDKDMYVGECQDYENFDQDRVS 457

Query: 443 GKVLLCGYSFSFVVGTASVKKVSQTAKALGAAGFVLAVENVSPGAKFDPVPVGIPGILIT 502
           GK+L+C YS  FV+G +++K+    AK L A G +  ++    G + +P P+ +PGI+I 
Sbjct: 458 GKLLICSYSARFVLGLSTIKQALDVAKNLSATGVIFYIDPYVLGFEINPTPMDMPGIIIP 517

Query: 503 DVSKSMDLIDYYNTSTPRDWTGR-VKSFEAVGRIGDGLMPLLYKSAPEVALFSARGPNIR 562
            V  S  L+ YYN+S  RD T + + SF AV  I  GL       AP+V  +SARGP+  
Sbjct: 518 SVEDSKTLLKYYNSSIQRDVTTKEIVSFGAVAAIEGGLNANFSNRAPKVMYYSARGPDPE 577

Query: 563 DFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIAGIAALV 622
           D SF DAD+LKP+++APG+ IW AWS   TD   + GE FAM+SGTSMAAPH+AG+AAL+
Sbjct: 578 DNSFNDADVLKPNLVAPGNSIWGAWSSASTDSTEFEGEKFAMMSGTSMAAPHVAGVAALI 637

Query: 623 KQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQCSETEAMKLVPATPFDYGSGAVNPRA 682
           KQ +P ++P+ I SAL TT+   D  G P+ AQ+        L  ATP D GSG VN  A
Sbjct: 638 KQSYPQFTPSTISSALSTTALLNDNKGSPIMAQRTYSNPDQSLYTATPSDMGSGFVNATA 697

Query: 683 ALDPGLIFDAGYEDYLGFLCTTAGINVHE--IHNYTS--SPCNFTMGHPWNLNSPSITIS 742
           ALDPGL+FD  +EDY+ FLC   GIN  +  + NYT    P N T    ++LN PSIT+S
Sbjct: 698 ALDPGLVFDTSFEDYISFLC---GINGSDTVVFNYTGFRCPANNTPVSGFDLNLPSITVS 757

Query: 743 HLVGTQTVTRTVTNVAEEETYVLTARMDPAVAIETDPPAMTLRSGSSRKFTVTLTARSLT 802
            L GTQT  R++ N+A  ETY +       V+++  P   ++  G ++  +VTLT    +
Sbjct: 758 TLSGTQTFQRSMRNIAGNETYNVGWSPPYGVSMKVSPTQFSIAMGENQVLSVTLTVTKNS 817

Query: 803 GTYSFGEVLMKGSRGHKVRIPVVAM 813
            + SFG + + G+ GH V IPV  +
Sbjct: 818 SSSSFGRIGLFGNTGHIVNIPVTVI 834

BLAST of MC08g2075 vs. ExPASy Swiss-Prot
Match: Q9SUN6 (Subtilisin-like protease SBT2.2 OS=Arabidopsis thaliana OX=3702 GN=SBT2.2 PE=3 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 3.8e-183
Identity = 350/747 (46.85%), Postives = 468/747 (62.65%), Query Frame = 0

Query: 72  HDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAEILRHTPIVKSVERDWKVRRLTTH 131
           HD LL    +   + KLYS+ +LINGFAV ++ +QAE L     V ++  D+ VR  TT+
Sbjct: 103 HDSLLRNALKGEKYIKLYSFHYLINGFAVFVSSQQAETLSRRREVANIVLDFSVRTATTY 162

Query: 132 TPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNT--EPFGPCLKYKGK 191
           TP+F+GLP G W   GG++ AGE IVIGF+D+GI P HPSF   +T    +     + G 
Sbjct: 163 TPQFMGLPKGAWVKEGGYETAGEGIVIGFIDTGIDPTHPSFNGTDTSQRQYPIPNHFSGV 222

Query: 192 CEVDPNTKKSFCNGKIIGAQHFAEAAKAAGAFNPSIHFASPLDGDGHGSHTAAIAAGNNG 251
           CEV P+     CN K++GA+HFA++A   G FN S  +ASP DGDGHG+HTA+IAAGN+G
Sbjct: 223 CEVTPDFPSGSCNRKLVGARHFAQSAITRGIFNSSEDYASPFDGDGHGTHTASIAAGNHG 282

Query: 252 IPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSVGP 311
           +   + G+ FG ASG+APRA I+VYKALY+ FGGF ADVVAAIDQA  DGVDILSLS+ P
Sbjct: 283 VSAVVSGHNFGSASGIAPRAHISVYKALYKSFGGFAADVVAAIDQAAQDGVDILSLSITP 342

Query: 312 NSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAIDDR 371
           N  P     T+ NP D  +LSAVKAG+FV QAAGN GP PK++ S+SPWI TV AA  DR
Sbjct: 343 NRRPPGV-ATFFNPLDMAMLSAVKAGIFVVQAAGNTGPSPKSMSSFSPWIFTVGAASHDR 402

Query: 372 RYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLIDSSVT---KYSPSDCQRPEVL 431
            Y N + LGN   + G+GL+  T   + YT+++A D L + S          +CQ     
Sbjct: 403 DYSNSIVLGNNVSIPGVGLALRTDEGKKYTMISALDALKNKSSVVDKDMYVGECQDYGSF 462

Query: 432 NKRLVEGKVLLCGYSFSFVVGTASVKKVSQTAKALGAAGFVLAVENVSPGAKFDPVPVGI 491
           +K ++ G +L+C YS  FV+G +++K+    AK L A G V  ++    G + +P P+ +
Sbjct: 463 DKDVIRGNLLICSYSIRFVLGLSTIKQALAVAKNLSAKGVVFYMDPYVLGFQINPTPMDM 522

Query: 492 PGILITDVSKSMDLIDYYNTSTPRDWTGR-VKSFEAVGRIGDGLMPLLYKSAPEVALFSA 551
           PGI+I     S  L+ YYN+S  RD T + +  F AV  I  G        AP++  +SA
Sbjct: 523 PGIIIPSAEDSKVLLKYYNSSLVRDGTTKEIVRFGAVAAIAGGQNANFSNRAPKIMYYSA 582

Query: 552 RGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIA 611
           RGP+ +D  F DAD+LKP+++APG+ IW AWS   T+   + GE FAM+SGTSMAAPH+A
Sbjct: 583 RGPDPQDSLFNDADILKPNLVAPGNSIWGAWSSAATESTEFEGESFAMMSGTSMAAPHVA 642

Query: 612 GIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQCSETEAMKLVPATPFDYGSG 671
           G+AALVKQK   +SP+AI SAL TTS   D  G  + AQ+        + PATPFD G+G
Sbjct: 643 GVAALVKQKFRKFSPSAIASALSTTSVLFDNKGEAIMAQRAYANPDQTISPATPFDMGNG 702

Query: 672 AVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTSSPC---NFTMGHPWNLNSP 731
            VN  AALDPGLIFD  +EDY+ FLC   G +   + NYT + C   N T+    +LN P
Sbjct: 703 FVNATAALDPGLIFDTSFEDYMSFLCGING-SAPVVFNYTGTNCLRNNATISGS-DLNLP 762

Query: 732 SITISHLVGTQTVTRTVTNVAEEETYVLTARMDPAVAIETDPPAMTLRSGSSRKFTVTLT 791
           SIT+S L  T+TV R +TN+A  ETY ++      V I   P   ++ SG ++  +V LT
Sbjct: 763 SITVSKLNNTRTVQRLMTNIAGNETYTVSLITPFDVLINVSPTQFSIASGETKLLSVILT 822

Query: 792 ARSLTGTYSFGEVLMKGSRGHKVRIPV 810
           A+  +   SFG + + G+ GH VRIPV
Sbjct: 823 AKRNSSISSFGGIKLLGNAGHIVRIPV 846

BLAST of MC08g2075 vs. ExPASy Swiss-Prot
Match: Q9SA75 (Subtilisin-like protease SBT2.1 OS=Arabidopsis thaliana OX=3702 GN=SBT2.1 PE=2 SV=1)

HSP 1 Score: 636.7 bits (1641), Expect = 3.5e-181
Identity = 359/810 (44.32%), Postives = 499/810 (61.60%), Query Frame = 0

Query: 23  IYIVSIEGEPIVSYRGDLDGFEATAVESDEKIDPTSELV---TSYARHLENKHDMLLGML 82
           +YIV+++  P V + G        + +S   +  TS  +    + +  +   HD LL  +
Sbjct: 36  VYIVTLKDRPSVHFSG------RESSDSKHSLTATSSQIYRTLNRSASIIRVHDSLLRNV 95

Query: 83  FERGSFKKLYSYKHLINGFAVDITPEQAEILRHTPIVKSVERDWKVRRLTTHTPEFLGLP 142
             + ++ KLYSY +LINGF+  +T +QA+ L     V++V  D+ V + TTHTP+FLGLP
Sbjct: 96  LRKENYLKLYSYHYLINGFSAVLTRKQADRLAAREEVENVVLDFLVEKATTHTPQFLGLP 155

Query: 143 TGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFA------TYNTEPFGPCLKYKGKCEVD 202
            G W   GG + AGE +VIGF+D+GI P HPSF+      TY+  P      + G CEV 
Sbjct: 156 RGAWLRDGGSEYAGEGVVIGFIDTGIDPTHPSFSDKISGHTYSVPP-----HFTGVCEVT 215

Query: 203 PNTKKSFCNGKIIGAQHFAEAAKAAGAFNPSIHFASPLDGDGHGSHTAAIAAGNNGIPVR 262
                  CN K+IGA+HFAE+A + G  N S   ASP DG+GHG+HTA++AAGN+GIPV 
Sbjct: 216 IGFPPGSCNRKLIGARHFAESALSRGVLNSSQDDASPFDGEGHGTHTASVAAGNHGIPVV 275

Query: 263 MHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPP 322
           + G+  G ASGMAPRA IA+YKALY+ FGGF AD++AAIDQA  DGVDI++LS+ PN  P
Sbjct: 276 VAGHRLGNASGMAPRAHIAIYKALYKRFGGFAADIIAAIDQAAQDGVDIINLSITPNRRP 335

Query: 323 ATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAIDDRRYKN 382
                T+ NP D  LLSAVKAG+FV QAAGN GP PK++ S+SPWI TV A   DR Y N
Sbjct: 336 PGI-ATFFNPIDMALLSAVKAGIFVVQAAGNTGPAPKSMSSFSPWIFTVGATSHDRVYSN 395

Query: 383 HLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLIDSSVTKYS--PSDCQRPEVLNKRLV 442
            + LGN   + G+GL+  T +   + LV A   L + +    +    +CQ     +++LV
Sbjct: 396 SIILGNNVTIPGVGLASGTRI--MHKLVLATHALRNGTTVMDAIYVGECQDSSSFDQKLV 455

Query: 443 EGKVLLCGYSFSFVVGTASVKKVSQTAKALGAAGFVLAVENVSPGAKFDPVPVGIPGILI 502
           +GK+L+C Y+  F++G +++K+   TAK L AAG V  ++  + G +    P+ IPGILI
Sbjct: 456 QGKILVCSYTVRFILGVSTIKQALLTAKNLTAAGLVFYIDPSATGFQMTSSPMDIPGILI 515

Query: 503 TDVSKSMDLIDYYNTSTPRD-WTGRVKSFEAVGRIGDGLMPLLYKSAPEVALFSARGPNI 562
           +    S  L+ YYN+S  R+  +G++    +V +I  G+ P    +AP+V  FSARGP+ 
Sbjct: 516 SSPQDSQALLRYYNSSLLRENGSGKIVGSASVAKIVGGMRPTYGITAPKVMYFSARGPDP 575

Query: 563 RDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIAGIAAL 622
            D SF DAD++KP+++APG+ IW AWSP G    ++ GE FAM SGTSM+APH+ GIAAL
Sbjct: 576 EDDSFVDADIMKPNLVAPGNAIWGAWSPLGIGTNDFQGERFAMESGTSMSAPHVTGIAAL 635

Query: 623 VKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQCSETEAMKLVPATPFDYGSGAVNPR 682
           +KQK P+++PAAI SAL TT++  DR G  + AQ+      +   PATPFD GSG VN  
Sbjct: 636 IKQKFPHFTPAAIASALSTTASLSDRKGEHIMAQRTVLNPDISQSPATPFDMGSGFVNAT 695

Query: 683 AALDPGLIFDAGYEDYLGFLCTTAGINVHE--IHNYTSSPC---NFTMGHPWNLNSPSIT 742
           AALDPGLIFD GY +Y+ FLC   GIN     + NYT   C   N ++    +LN PS+T
Sbjct: 696 AALDPGLIFDIGYNEYMKFLC---GINGSSPVVLNYTGESCSSYNSSLAAS-DLNLPSVT 755

Query: 743 ISHLVGTQTVTRTVTNV---AEEETYVLTARMDPAVAIETDPPAMTLRSGSSRKFTVTLT 802
           I+ LVGT+ V R VTN+   A  ETY++      +V+++  P   T+ +G +R  ++   
Sbjct: 756 IAKLVGTRAVLRWVTNIATTATNETYIVGWMAPDSVSVKVSPAKFTIGNGQTRVLSLVFR 815

Query: 803 ARSLTGTYSFGEVLMKGSRGHKVRIPVVAM 813
           A       SFG + + G RGH V IPV  +
Sbjct: 816 AMKNVSMASFGRIGLFGDRGHVVNIPVAVI 827

BLAST of MC08g2075 vs. NCBI nr
Match: XP_022149854.1 (subtilisin-like protease SBT2.5 [Momordica charantia])

HSP 1 Score: 1630 bits (4220), Expect = 0.0
Identity = 816/816 (100.00%), Postives = 816/816 (100.00%), Query Frame = 0

Query: 1   MVVGFQQAVLVFLALLVVGRADIYIVSIEGEPIVSYRGDLDGFEATAVESDEKIDPTSEL 60
           MVVGFQQAVLVFLALLVVGRADIYIVSIEGEPIVSYRGDLDGFEATAVESDEKIDPTSEL
Sbjct: 1   MVVGFQQAVLVFLALLVVGRADIYIVSIEGEPIVSYRGDLDGFEATAVESDEKIDPTSEL 60

Query: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAEILRHTPIVKSVE 120
           VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAEILRHTPIVKSVE
Sbjct: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAEILRHTPIVKSVE 120

Query: 121 RDWKVRRLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180
           RDWKVRRLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF
Sbjct: 121 RDWKVRRLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 181 GPCLKYKGKCEVDPNTKKSFCNGKIIGAQHFAEAAKAAGAFNPSIHFASPLDGDGHGSHT 240
           GPCLKYKGKCEVDPNTKKSFCNGKIIGAQHFAEAAKAAGAFNPSIHFASPLDGDGHGSHT
Sbjct: 181 GPCLKYKGKCEVDPNTKKSFCNGKIIGAQHFAEAAKAAGAFNPSIHFASPLDGDGHGSHT 240

Query: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLIDSSVTKYSPSDC 420
           TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLIDSSVTKYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLIDSSVTKYSPSDC 420

Query: 421 QRPEVLNKRLVEGKVLLCGYSFSFVVGTASVKKVSQTAKALGAAGFVLAVENVSPGAKFD 480
           QRPEVLNKRLVEGKVLLCGYSFSFVVGTASVKKVSQTAKALGAAGFVLAVENVSPGAKFD
Sbjct: 421 QRPEVLNKRLVEGKVLLCGYSFSFVVGTASVKKVSQTAKALGAAGFVLAVENVSPGAKFD 480

Query: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFEAVGRIGDGLMPLLYKSAPEV 540
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFEAVGRIGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFEAVGRIGDGLMPLLYKSAPEV 540

Query: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQCSETEAMKLVPATPF 660
           APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQCSETEAMKLVPATPF
Sbjct: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQCSETEAMKLVPATPF 660

Query: 661 DYGSGAVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTSSPCNFTMGHPWNLN 720
           DYGSGAVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTSSPCNFTMGHPWNLN
Sbjct: 661 DYGSGAVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTSSPCNFTMGHPWNLN 720

Query: 721 SPSITISHLVGTQTVTRTVTNVAEEETYVLTARMDPAVAIETDPPAMTLRSGSSRKFTVT 780
           SPSITISHLVGTQTVTRTVTNVAEEETYVLTARMDPAVAIETDPPAMTLRSGSSRKFTVT
Sbjct: 721 SPSITISHLVGTQTVTRTVTNVAEEETYVLTARMDPAVAIETDPPAMTLRSGSSRKFTVT 780

Query: 781 LTARSLTGTYSFGEVLMKGSRGHKVRIPVVAMGYQR 816
           LTARSLTGTYSFGEVLMKGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTARSLTGTYSFGEVLMKGSRGHKVRIPVVAMGYQR 816

BLAST of MC08g2075 vs. NCBI nr
Match: XP_038890136.1 (subtilisin-like protease SBT2.5 isoform X1 [Benincasa hispida] >XP_038890137.1 subtilisin-like protease SBT2.5 isoform X1 [Benincasa hispida] >XP_038890138.1 subtilisin-like protease SBT2.5 isoform X1 [Benincasa hispida])

HSP 1 Score: 1567 bits (4058), Expect = 0.0
Identity = 778/816 (95.34%), Postives = 802/816 (98.28%), Query Frame = 0

Query: 1   MVVGFQQAVLVFLALLVVGRADIYIVSIEGEPIVSYRGDLDGFEATAVESDEKIDPTSEL 60
           MVV FQ +VLVFLA+LVVG+A+IYIV+IEGEPIVSY+GDLDGFEATA+ESDEKIDPTSE+
Sbjct: 1   MVVNFQCSVLVFLAILVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESDEKIDPTSEI 60

Query: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAEILRHTPIVKSVE 120
           VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDIT EQAEILRHTPIVKSVE
Sbjct: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAEILRHTPIVKSVE 120

Query: 121 RDWKVRRLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180
           RDWKVRRLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGI PHHPSFATYNTEPF
Sbjct: 121 RDWKVRRLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGISPHHPSFATYNTEPF 180

Query: 181 GPCLKYKGKCEVDPNTKKSFCNGKIIGAQHFAEAAKAAGAFNPSIHFASPLDGDGHGSHT 240
           GPCLKYKGKCEVDP+TKK FCNGKI+GAQHFAEAAKAAGAFNP+IHFASPLDGDGHGSHT
Sbjct: 181 GPCLKYKGKCEVDPDTKKDFCNGKIVGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHT 240

Query: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLIDSSVTKYSPSDC 420
           TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVL+DSSV KYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVMKYSPSDC 420

Query: 421 QRPEVLNKRLVEGKVLLCGYSFSFVVGTASVKKVSQTAKALGAAGFVLAVENVSPGAKFD 480
           Q+PEVLNKRLVEGKVLLCGYSFSFVVGTAS+KKVSQTAKALGAAGFVLAVEN+SPGAKFD
Sbjct: 421 QKPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD 480

Query: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFEAVGRIGDGLMPLLYKSAPEV 540
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSF+AVG IGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQCSETEAMKLVPATPF 660
           APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDR GRPLKAQQ SETEAMKLV ATPF
Sbjct: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVSATPF 660

Query: 661 DYGSGAVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTSSPCNFTMGHPWNLN 720
           DYGSG VNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYT+SPCNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 720

Query: 721 SPSITISHLVGTQTVTRTVTNVAEEETYVLTARMDPAVAIETDPPAMTLRSGSSRKFTVT 780
           SPSITI+HLVG+QTVTRTVTNVAEEETY +TARMDPAVAIE +PPAMTL SGSSRKF+VT
Sbjct: 721 SPSITIAHLVGSQTVTRTVTNVAEEETYTITARMDPAVAIEVNPPAMTLLSGSSRKFSVT 780

Query: 781 LTARSLTGTYSFGEVLMKGSRGHKVRIPVVAMGYQR 816
           LTARSLTGTYSFG+VL+KGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of MC08g2075 vs. NCBI nr
Match: XP_023546345.1 (subtilisin-like protease SBT2.5 [Cucurbita pepo subsp. pepo] >XP_023546347.1 subtilisin-like protease SBT2.5 [Cucurbita pepo subsp. pepo] >XP_023546348.1 subtilisin-like protease SBT2.5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1565 bits (4051), Expect = 0.0
Identity = 773/816 (94.73%), Postives = 803/816 (98.41%), Query Frame = 0

Query: 1   MVVGFQQAVLVFLALLVVGRADIYIVSIEGEPIVSYRGDLDGFEATAVESDEKIDPTSEL 60
           MVV FQ  VLVFL L+VVG+A+IYIV+IEGEPIVSY+GDLDGFEATA+ES+EKIDPTSE+
Sbjct: 1   MVVNFQCTVLVFLVLVVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESNEKIDPTSEI 60

Query: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAEILRHTPIVKSVE 120
           VTSYARHLE+KHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAE LRHTPIVKSVE
Sbjct: 61  VTSYARHLESKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAETLRHTPIVKSVE 120

Query: 121 RDWKVRRLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180
           RDWKVR+LTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF
Sbjct: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 181 GPCLKYKGKCEVDPNTKKSFCNGKIIGAQHFAEAAKAAGAFNPSIHFASPLDGDGHGSHT 240
           GPCLKYKGKCEVDP+TKK FCNGKI+GAQHFAEAAKAAGAFNP+IHF SPLDGDGHGSHT
Sbjct: 181 GPCLKYKGKCEVDPDTKKEFCNGKIVGAQHFAEAAKAAGAFNPAIHFESPLDGDGHGSHT 240

Query: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLIDSSVTKYSPSDC 420
           TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVL+DSSV+KYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVSKYSPSDC 420

Query: 421 QRPEVLNKRLVEGKVLLCGYSFSFVVGTASVKKVSQTAKALGAAGFVLAVENVSPGAKFD 480
           QRP+VLNKRLVEGKVLLCGYSFSFVVGTAS+KKVSQTA+ALGAAGFVLAVEN+SPG+KFD
Sbjct: 421 QRPDVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTARALGAAGFVLAVENISPGSKFD 480

Query: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFEAVGRIGDGLMPLLYKSAPEV 540
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSF+AVG IGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQCSETEAMKLVPATPF 660
           APHIAGI+ALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQ SETEAMKLV ATPF
Sbjct: 601 APHIAGISALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVSATPF 660

Query: 661 DYGSGAVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTSSPCNFTMGHPWNLN 720
           DYGSG VNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHN+T+SPCNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNFTNSPCNFTMGHPWNLN 720

Query: 721 SPSITISHLVGTQTVTRTVTNVAEEETYVLTARMDPAVAIETDPPAMTLRSGSSRKFTVT 780
           +PSITI+HLVGT+TVTRTVTNVAEEETY +TARMDPAVAIET PPAMTLRSGSSRKF+VT
Sbjct: 721 TPSITIAHLVGTKTVTRTVTNVAEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVT 780

Query: 781 LTARSLTGTYSFGEVLMKGSRGHKVRIPVVAMGYQR 816
           LTARSLTGTYSFGEVL+KGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTARSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of MC08g2075 vs. NCBI nr
Match: XP_022996483.1 (subtilisin-like protease SBT2.5 isoform X1 [Cucurbita maxima] >XP_022996484.1 subtilisin-like protease SBT2.5 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1564 bits (4049), Expect = 0.0
Identity = 773/816 (94.73%), Postives = 802/816 (98.28%), Query Frame = 0

Query: 1   MVVGFQQAVLVFLALLVVGRADIYIVSIEGEPIVSYRGDLDGFEATAVESDEKIDPTSEL 60
           MVV FQ  VLVFL LLVVG+A+IYIV+IEGEPIVSY+GDLDGFEATA+ES+EKIDPTSE+
Sbjct: 1   MVVNFQCTVLVFLVLLVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESNEKIDPTSEI 60

Query: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAEILRHTPIVKSVE 120
           VTSYARHLE+KHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAE LRHTPIVKSVE
Sbjct: 61  VTSYARHLESKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAETLRHTPIVKSVE 120

Query: 121 RDWKVRRLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180
            DWKVR+LTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF
Sbjct: 121 MDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 181 GPCLKYKGKCEVDPNTKKSFCNGKIIGAQHFAEAAKAAGAFNPSIHFASPLDGDGHGSHT 240
           GPCLKYKGKCEVDP+TKK FCNGKI+GAQHFAEAAKAAGAFNP+IHF SPLDGDGHGSHT
Sbjct: 181 GPCLKYKGKCEVDPDTKKEFCNGKIVGAQHFAEAAKAAGAFNPAIHFESPLDGDGHGSHT 240

Query: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLIDSSVTKYSPSDC 420
           TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVL+DSSV+KYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVSKYSPSDC 420

Query: 421 QRPEVLNKRLVEGKVLLCGYSFSFVVGTASVKKVSQTAKALGAAGFVLAVENVSPGAKFD 480
           QRP+VLNKRLVEGKVLLCGYSFSFVVGTAS+KKVSQTA+ALGAAGFVLAVEN+SPG+KFD
Sbjct: 421 QRPDVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTARALGAAGFVLAVENISPGSKFD 480

Query: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFEAVGRIGDGLMPLLYKSAPEV 540
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSF+AVG IGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQCSETEAMKLVPATPF 660
           APHIAGI+ALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQ SETEAMKLV ATPF
Sbjct: 601 APHIAGISALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVSATPF 660

Query: 661 DYGSGAVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTSSPCNFTMGHPWNLN 720
           DYGSG VNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYT+SPCNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 720

Query: 721 SPSITISHLVGTQTVTRTVTNVAEEETYVLTARMDPAVAIETDPPAMTLRSGSSRKFTVT 780
           +PSITI+HLVGT+TVTRTVTNVAEEETY +TARMDPAVAIET PPAMTLRSGSSRKF+VT
Sbjct: 721 TPSITIAHLVGTKTVTRTVTNVAEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVT 780

Query: 781 LTARSLTGTYSFGEVLMKGSRGHKVRIPVVAMGYQR 816
           LT+RSLTGTYSFGEVL+KGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTSRSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of MC08g2075 vs. NCBI nr
Match: XP_022929689.1 (subtilisin-like protease SBT2.5 [Cucurbita moschata] >XP_022929690.1 subtilisin-like protease SBT2.5 [Cucurbita moschata] >XP_022929691.1 subtilisin-like protease SBT2.5 [Cucurbita moschata])

HSP 1 Score: 1562 bits (4045), Expect = 0.0
Identity = 771/816 (94.49%), Postives = 802/816 (98.28%), Query Frame = 0

Query: 1   MVVGFQQAVLVFLALLVVGRADIYIVSIEGEPIVSYRGDLDGFEATAVESDEKIDPTSEL 60
           MVV FQ  VLVFL L+VVG+A+IYIV+IEGEPIVSY+GDLDGFEATA+ES+EKIDPTSE+
Sbjct: 1   MVVNFQCTVLVFLVLVVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESNEKIDPTSEI 60

Query: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAEILRHTPIVKSVE 120
           VTSYARHLE+KHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAE LRHTPIVKSVE
Sbjct: 61  VTSYARHLESKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAETLRHTPIVKSVE 120

Query: 121 RDWKVRRLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180
           RDWKVR+LTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF
Sbjct: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 181 GPCLKYKGKCEVDPNTKKSFCNGKIIGAQHFAEAAKAAGAFNPSIHFASPLDGDGHGSHT 240
           GPCLKYKGKCEVDP+TKK FCNGKI+GAQHFAEAAKAAGAFNP+IHF SPLDGDGHGSHT
Sbjct: 181 GPCLKYKGKCEVDPDTKKEFCNGKIVGAQHFAEAAKAAGAFNPAIHFESPLDGDGHGSHT 240

Query: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLIDSSVTKYSPSDC 420
           TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATH NRTYTLVAANDVL+DSSV+KYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHFNRTYTLVAANDVLLDSSVSKYSPSDC 420

Query: 421 QRPEVLNKRLVEGKVLLCGYSFSFVVGTASVKKVSQTAKALGAAGFVLAVENVSPGAKFD 480
           QRP+VLNKRLVEGKVLLCGYSFSFVVGTAS+KKVSQTA+ALGAAGFVLAVEN+SPG+KFD
Sbjct: 421 QRPDVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTARALGAAGFVLAVENISPGSKFD 480

Query: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFEAVGRIGDGLMPLLYKSAPEV 540
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSF+AVG IGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQCSETEAMKLVPATPF 660
           APHIAGI+ALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQ SETEAMKLV ATPF
Sbjct: 601 APHIAGISALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVSATPF 660

Query: 661 DYGSGAVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTSSPCNFTMGHPWNLN 720
           DYGSG VNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHN+T+SPCNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNHTNSPCNFTMGHPWNLN 720

Query: 721 SPSITISHLVGTQTVTRTVTNVAEEETYVLTARMDPAVAIETDPPAMTLRSGSSRKFTVT 780
           +PSITI+HLVGT+TVTRTVTNVAEEETY +TARMDPA+AIET PPAMTLRSGSSRKF+VT
Sbjct: 721 TPSITIAHLVGTKTVTRTVTNVAEEETYTITARMDPAIAIETSPPAMTLRSGSSRKFSVT 780

Query: 781 LTARSLTGTYSFGEVLMKGSRGHKVRIPVVAMGYQR 816
           LTARSLTGTYSFGEVL+KGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTARSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of MC08g2075 vs. ExPASy TrEMBL
Match: A0A6J1D895 (subtilisin-like protease SBT2.5 OS=Momordica charantia OX=3673 GN=LOC111018183 PE=3 SV=1)

HSP 1 Score: 1630 bits (4220), Expect = 0.0
Identity = 816/816 (100.00%), Postives = 816/816 (100.00%), Query Frame = 0

Query: 1   MVVGFQQAVLVFLALLVVGRADIYIVSIEGEPIVSYRGDLDGFEATAVESDEKIDPTSEL 60
           MVVGFQQAVLVFLALLVVGRADIYIVSIEGEPIVSYRGDLDGFEATAVESDEKIDPTSEL
Sbjct: 1   MVVGFQQAVLVFLALLVVGRADIYIVSIEGEPIVSYRGDLDGFEATAVESDEKIDPTSEL 60

Query: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAEILRHTPIVKSVE 120
           VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAEILRHTPIVKSVE
Sbjct: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAEILRHTPIVKSVE 120

Query: 121 RDWKVRRLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180
           RDWKVRRLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF
Sbjct: 121 RDWKVRRLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 181 GPCLKYKGKCEVDPNTKKSFCNGKIIGAQHFAEAAKAAGAFNPSIHFASPLDGDGHGSHT 240
           GPCLKYKGKCEVDPNTKKSFCNGKIIGAQHFAEAAKAAGAFNPSIHFASPLDGDGHGSHT
Sbjct: 181 GPCLKYKGKCEVDPNTKKSFCNGKIIGAQHFAEAAKAAGAFNPSIHFASPLDGDGHGSHT 240

Query: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLIDSSVTKYSPSDC 420
           TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLIDSSVTKYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLIDSSVTKYSPSDC 420

Query: 421 QRPEVLNKRLVEGKVLLCGYSFSFVVGTASVKKVSQTAKALGAAGFVLAVENVSPGAKFD 480
           QRPEVLNKRLVEGKVLLCGYSFSFVVGTASVKKVSQTAKALGAAGFVLAVENVSPGAKFD
Sbjct: 421 QRPEVLNKRLVEGKVLLCGYSFSFVVGTASVKKVSQTAKALGAAGFVLAVENVSPGAKFD 480

Query: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFEAVGRIGDGLMPLLYKSAPEV 540
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFEAVGRIGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFEAVGRIGDGLMPLLYKSAPEV 540

Query: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQCSETEAMKLVPATPF 660
           APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQCSETEAMKLVPATPF
Sbjct: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQCSETEAMKLVPATPF 660

Query: 661 DYGSGAVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTSSPCNFTMGHPWNLN 720
           DYGSGAVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTSSPCNFTMGHPWNLN
Sbjct: 661 DYGSGAVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTSSPCNFTMGHPWNLN 720

Query: 721 SPSITISHLVGTQTVTRTVTNVAEEETYVLTARMDPAVAIETDPPAMTLRSGSSRKFTVT 780
           SPSITISHLVGTQTVTRTVTNVAEEETYVLTARMDPAVAIETDPPAMTLRSGSSRKFTVT
Sbjct: 721 SPSITISHLVGTQTVTRTVTNVAEEETYVLTARMDPAVAIETDPPAMTLRSGSSRKFTVT 780

Query: 781 LTARSLTGTYSFGEVLMKGSRGHKVRIPVVAMGYQR 816
           LTARSLTGTYSFGEVLMKGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTARSLTGTYSFGEVLMKGSRGHKVRIPVVAMGYQR 816

BLAST of MC08g2075 vs. ExPASy TrEMBL
Match: A0A6J1KAV8 (subtilisin-like protease SBT2.5 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491718 PE=3 SV=1)

HSP 1 Score: 1564 bits (4049), Expect = 0.0
Identity = 773/816 (94.73%), Postives = 802/816 (98.28%), Query Frame = 0

Query: 1   MVVGFQQAVLVFLALLVVGRADIYIVSIEGEPIVSYRGDLDGFEATAVESDEKIDPTSEL 60
           MVV FQ  VLVFL LLVVG+A+IYIV+IEGEPIVSY+GDLDGFEATA+ES+EKIDPTSE+
Sbjct: 1   MVVNFQCTVLVFLVLLVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESNEKIDPTSEI 60

Query: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAEILRHTPIVKSVE 120
           VTSYARHLE+KHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAE LRHTPIVKSVE
Sbjct: 61  VTSYARHLESKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAETLRHTPIVKSVE 120

Query: 121 RDWKVRRLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180
            DWKVR+LTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF
Sbjct: 121 MDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 181 GPCLKYKGKCEVDPNTKKSFCNGKIIGAQHFAEAAKAAGAFNPSIHFASPLDGDGHGSHT 240
           GPCLKYKGKCEVDP+TKK FCNGKI+GAQHFAEAAKAAGAFNP+IHF SPLDGDGHGSHT
Sbjct: 181 GPCLKYKGKCEVDPDTKKEFCNGKIVGAQHFAEAAKAAGAFNPAIHFESPLDGDGHGSHT 240

Query: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLIDSSVTKYSPSDC 420
           TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVL+DSSV+KYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVSKYSPSDC 420

Query: 421 QRPEVLNKRLVEGKVLLCGYSFSFVVGTASVKKVSQTAKALGAAGFVLAVENVSPGAKFD 480
           QRP+VLNKRLVEGKVLLCGYSFSFVVGTAS+KKVSQTA+ALGAAGFVLAVEN+SPG+KFD
Sbjct: 421 QRPDVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTARALGAAGFVLAVENISPGSKFD 480

Query: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFEAVGRIGDGLMPLLYKSAPEV 540
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSF+AVG IGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQCSETEAMKLVPATPF 660
           APHIAGI+ALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQ SETEAMKLV ATPF
Sbjct: 601 APHIAGISALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVSATPF 660

Query: 661 DYGSGAVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTSSPCNFTMGHPWNLN 720
           DYGSG VNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYT+SPCNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 720

Query: 721 SPSITISHLVGTQTVTRTVTNVAEEETYVLTARMDPAVAIETDPPAMTLRSGSSRKFTVT 780
           +PSITI+HLVGT+TVTRTVTNVAEEETY +TARMDPAVAIET PPAMTLRSGSSRKF+VT
Sbjct: 721 TPSITIAHLVGTKTVTRTVTNVAEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVT 780

Query: 781 LTARSLTGTYSFGEVLMKGSRGHKVRIPVVAMGYQR 816
           LT+RSLTGTYSFGEVL+KGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTSRSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of MC08g2075 vs. ExPASy TrEMBL
Match: A0A6J1EPH6 (subtilisin-like protease SBT2.5 OS=Cucurbita moschata OX=3662 GN=LOC111436195 PE=3 SV=1)

HSP 1 Score: 1562 bits (4045), Expect = 0.0
Identity = 771/816 (94.49%), Postives = 802/816 (98.28%), Query Frame = 0

Query: 1   MVVGFQQAVLVFLALLVVGRADIYIVSIEGEPIVSYRGDLDGFEATAVESDEKIDPTSEL 60
           MVV FQ  VLVFL L+VVG+A+IYIV+IEGEPIVSY+GDLDGFEATA+ES+EKIDPTSE+
Sbjct: 1   MVVNFQCTVLVFLVLVVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESNEKIDPTSEI 60

Query: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAEILRHTPIVKSVE 120
           VTSYARHLE+KHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAE LRHTPIVKSVE
Sbjct: 61  VTSYARHLESKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAETLRHTPIVKSVE 120

Query: 121 RDWKVRRLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180
           RDWKVR+LTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF
Sbjct: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 181 GPCLKYKGKCEVDPNTKKSFCNGKIIGAQHFAEAAKAAGAFNPSIHFASPLDGDGHGSHT 240
           GPCLKYKGKCEVDP+TKK FCNGKI+GAQHFAEAAKAAGAFNP+IHF SPLDGDGHGSHT
Sbjct: 181 GPCLKYKGKCEVDPDTKKEFCNGKIVGAQHFAEAAKAAGAFNPAIHFESPLDGDGHGSHT 240

Query: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLIDSSVTKYSPSDC 420
           TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATH NRTYTLVAANDVL+DSSV+KYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHFNRTYTLVAANDVLLDSSVSKYSPSDC 420

Query: 421 QRPEVLNKRLVEGKVLLCGYSFSFVVGTASVKKVSQTAKALGAAGFVLAVENVSPGAKFD 480
           QRP+VLNKRLVEGKVLLCGYSFSFVVGTAS+KKVSQTA+ALGAAGFVLAVEN+SPG+KFD
Sbjct: 421 QRPDVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTARALGAAGFVLAVENISPGSKFD 480

Query: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFEAVGRIGDGLMPLLYKSAPEV 540
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSF+AVG IGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQCSETEAMKLVPATPF 660
           APHIAGI+ALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQ SETEAMKLV ATPF
Sbjct: 601 APHIAGISALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVSATPF 660

Query: 661 DYGSGAVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTSSPCNFTMGHPWNLN 720
           DYGSG VNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHN+T+SPCNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNHTNSPCNFTMGHPWNLN 720

Query: 721 SPSITISHLVGTQTVTRTVTNVAEEETYVLTARMDPAVAIETDPPAMTLRSGSSRKFTVT 780
           +PSITI+HLVGT+TVTRTVTNVAEEETY +TARMDPA+AIET PPAMTLRSGSSRKF+VT
Sbjct: 721 TPSITIAHLVGTKTVTRTVTNVAEEETYTITARMDPAIAIETSPPAMTLRSGSSRKFSVT 780

Query: 781 LTARSLTGTYSFGEVLMKGSRGHKVRIPVVAMGYQR 816
           LTARSLTGTYSFGEVL+KGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTARSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of MC08g2075 vs. ExPASy TrEMBL
Match: A0A5D3CEZ7 (Subtilisin-like protease SBT2.5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1411G00120 PE=3 SV=1)

HSP 1 Score: 1561 bits (4041), Expect = 0.0
Identity = 772/816 (94.61%), Postives = 798/816 (97.79%), Query Frame = 0

Query: 1   MVVGFQQAVLVFLALLVVGRADIYIVSIEGEPIVSYRGDLDGFEATAVESDEKIDPTSEL 60
           MVV FQ  VLVFLA+L VG+A+IYIV+I+GEPIVSY+GDLDGFEATA+ESDEKIDPTSE+
Sbjct: 1   MVVNFQYTVLVFLAILFVGKAEIYIVTIDGEPIVSYKGDLDGFEATAMESDEKIDPTSEI 60

Query: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAEILRHTPIVKSVE 120
           VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDIT EQAE LR TPIVKSVE
Sbjct: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAETLRRTPIVKSVE 120

Query: 121 RDWKVRRLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180
           RDWKVR+LTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF
Sbjct: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 181 GPCLKYKGKCEVDPNTKKSFCNGKIIGAQHFAEAAKAAGAFNPSIHFASPLDGDGHGSHT 240
           GPC+KYKGKCEVDPNTKK FCNGKI+GA+HFAEAAKAAGAFNP IHFASPLDGDGHGSHT
Sbjct: 181 GPCMKYKGKCEVDPNTKKDFCNGKIVGARHFAEAAKAAGAFNPDIHFASPLDGDGHGSHT 240

Query: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLIDSSVTKYSPSDC 420
           TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVL+DSSVTKYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDC 420

Query: 421 QRPEVLNKRLVEGKVLLCGYSFSFVVGTASVKKVSQTAKALGAAGFVLAVENVSPGAKFD 480
           Q+PE+LNK LVEGKVLLCGYSFSFVVGTAS+KKVSQTAKALGAAGFVLAVEN+SPGAKFD
Sbjct: 421 QKPELLNKHLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD 480

Query: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFEAVGRIGDGLMPLLYKSAPEV 540
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSF+AVG IGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQCSETEAMKLVPATPF 660
           APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDR GRPLKAQQ SETEAMKLV ATPF
Sbjct: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVTATPF 660

Query: 661 DYGSGAVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTSSPCNFTMGHPWNLN 720
           DYGSG VNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYT+SPCNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 720

Query: 721 SPSITISHLVGTQTVTRTVTNVAEEETYVLTARMDPAVAIETDPPAMTLRSGSSRKFTVT 780
           SPSITI+HLVGT+ VTRTVTNVAEEETY +TARMDPAVAIE +PPAMTLRSGSSRKF+VT
Sbjct: 721 SPSITIAHLVGTKIVTRTVTNVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVT 780

Query: 781 LTARSLTGTYSFGEVLMKGSRGHKVRIPVVAMGYQR 816
           LTARSLTGTYSFG+VL+KGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of MC08g2075 vs. ExPASy TrEMBL
Match: A0A1S3BRZ6 (subtilisin-like protease SBT2.5 OS=Cucumis melo OX=3656 GN=LOC103492606 PE=3 SV=1)

HSP 1 Score: 1560 bits (4040), Expect = 0.0
Identity = 772/816 (94.61%), Postives = 798/816 (97.79%), Query Frame = 0

Query: 1   MVVGFQQAVLVFLALLVVGRADIYIVSIEGEPIVSYRGDLDGFEATAVESDEKIDPTSEL 60
           MVV FQ  VLVFLA+L VG+A+IYIV+I+GEPIVSY+GDLDGFEATA+ESDEKIDPTSE+
Sbjct: 1   MVVNFQYTVLVFLAILFVGKAEIYIVTIDGEPIVSYKGDLDGFEATAMESDEKIDPTSEI 60

Query: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAEILRHTPIVKSVE 120
           VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDIT EQAE LR TPIVKSVE
Sbjct: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAESLRRTPIVKSVE 120

Query: 121 RDWKVRRLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180
           RDWKVR+LTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF
Sbjct: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 181 GPCLKYKGKCEVDPNTKKSFCNGKIIGAQHFAEAAKAAGAFNPSIHFASPLDGDGHGSHT 240
           GPC+KYKGKCEVDPNTKK FCNGKI+GA+HFAEAAKAAGAFNP IHFASPLDGDGHGSHT
Sbjct: 181 GPCMKYKGKCEVDPNTKKDFCNGKIVGARHFAEAAKAAGAFNPDIHFASPLDGDGHGSHT 240

Query: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLIDSSVTKYSPSDC 420
           TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVL+DSSVTKYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDC 420

Query: 421 QRPEVLNKRLVEGKVLLCGYSFSFVVGTASVKKVSQTAKALGAAGFVLAVENVSPGAKFD 480
           Q+PE+LNK LVEGKVLLCGYSFSFVVGTAS+KKVSQTAKALGAAGFVLAVEN+SPGAKFD
Sbjct: 421 QKPELLNKHLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD 480

Query: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFEAVGRIGDGLMPLLYKSAPEV 540
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSF+AVG IGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQCSETEAMKLVPATPF 660
           APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDR GRPLKAQQ SETEAMKLV ATPF
Sbjct: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVTATPF 660

Query: 661 DYGSGAVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTSSPCNFTMGHPWNLN 720
           DYGSG VNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYT+SPCNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 720

Query: 721 SPSITISHLVGTQTVTRTVTNVAEEETYVLTARMDPAVAIETDPPAMTLRSGSSRKFTVT 780
           SPSITI+HLVGT+ VTRTVTNVAEEETY +TARMDPAVAIE +PPAMTLRSGSSRKF+VT
Sbjct: 721 SPSITIAHLVGTKIVTRTVTNVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVT 780

Query: 781 LTARSLTGTYSFGEVLMKGSRGHKVRIPVVAMGYQR 816
           LTARSLTGTYSFG+VL+KGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of MC08g2075 vs. TAIR 10
Match: AT2G19170.1 (subtilisin-like serine protease 3 )

HSP 1 Score: 1344.3 bits (3478), Expect = 0.0e+00
Identity = 659/809 (81.46%), Postives = 728/809 (89.99%), Query Frame = 0

Query: 9   VLVFLALLVVGRADIYIVSIEGEPIVSYRGDLDGFEATAVESDEKIDPTSELVTSYARHL 68
           + V   LLV   A++YIV++EG+PI+SY+G  +GFEATAVESDEKID +SELVT YARHL
Sbjct: 7   IFVVFVLLVAVTAEVYIVTMEGDPIISYKGGENGFEATAVESDEKIDTSSELVTVYARHL 66

Query: 69  ENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAEILRHTPIVKSVERDWKVRRL 128
           E KHDM+LGMLFE GS+KKLYSYKHLINGFA  ++PEQAE LR  P V+SV++DWKVRRL
Sbjct: 67  ERKHDMILGMLFEEGSYKKLYSYKHLINGFAAHVSPEQAETLRRAPGVRSVDKDWKVRRL 126

Query: 129 TTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPFGPCLKYKG 188
           TTHTPEFLGLPT VWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFA+++  P+GP   YKG
Sbjct: 127 TTHTPEFLGLPTDVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFASHHRLPYGPLPHYKG 186

Query: 189 KCEVDPNTKKSFCNGKIIGAQHFAEAAKAAGAFNPSIHFASPLDGDGHGSHTAAIAAGNN 248
           KCE DP+TKKSFCN KI+GAQHFAEAAKAAGAFNP I +ASP+DGDGHGSHTAAIAAGNN
Sbjct: 187 KCEEDPHTKKSFCNRKIVGAQHFAEAAKAAGAFNPDIDYASPMDGDGHGSHTAAIAAGNN 246

Query: 249 GIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSVG 308
           GIP+RMHGYEFGKASGMAPRARIAVYKALYR+FGGFVADVVAAIDQAVHDGVDILSLSVG
Sbjct: 247 GIPLRMHGYEFGKASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSVG 306

Query: 309 PNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAIDD 368
           PNSPP TTK T+LNPFDATLL AVKAGVFVAQAAGNGGPFPKTLVSYSPWI TVAAAIDD
Sbjct: 307 PNSPPTTTKTTFLNPFDATLLGAVKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAIDD 366

Query: 369 RRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLIDSSVTKYSPSDCQRPEVLNK 428
           RRYKNHLTLGNGK+LAG+GLSP T  +R YTLV+ANDVL+DSSV+KY+PSDCQRPEV NK
Sbjct: 367 RRYKNHLTLGNGKMLAGMGLSPPTRPHRLYTLVSANDVLLDSSVSKYNPSDCQRPEVFNK 426

Query: 429 RLVEGKVLLCGYSFSFVVGTASVKKVSQTAKALGAAGFVLAVENVSPGAKFDPVPVGIPG 488
           +LVEG +LLCGYSF+FVVGTAS+KKV  TAK LGAAGFVL VENVSPG KFDPVP  IPG
Sbjct: 427 KLVEGNILLCGYSFNFVVGTASIKKVVATAKHLGAAGFVLVVENVSPGTKFDPVPSAIPG 486

Query: 489 ILITDVSKSMDLIDYYNTSTPRDWTGRVKSFEAVGRIGDGLMPLLYKSAPEVALFSARGP 548
           ILITDVSKSMDLIDYYN ST RDWTGRVKSF+A G IGDGL P+L+KSAP+VALFSARGP
Sbjct: 487 ILITDVSKSMDLIDYYNASTSRDWTGRVKSFKAEGSIGDGLAPVLHKSAPQVALFSARGP 546

Query: 549 NIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIAGIA 608
           N +DFSFQDADLLKPDILAPG LIWAAW PNGTDEPNYVGEGFA+ISGTSMAAPHIAGIA
Sbjct: 547 NTKDFSFQDADLLKPDILAPGYLIWAAWCPNGTDEPNYVGEGFALISGTSMAAPHIAGIA 606

Query: 609 ALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQCSETEAMKLVPATPFDYGSGAVN 668
           ALVKQKHP WSPAAIKSALMTTST +DRAGR L+AQQ S+TEA+ LV ATPFDYGSG VN
Sbjct: 607 ALVKQKHPQWSPAAIKSALMTTSTVIDRAGRLLQAQQYSDTEAVTLVKATPFDYGSGHVN 666

Query: 669 PRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTSSPCNFTMGHPWNLNSPSITISH 728
           P AALDPGLIFDAGYEDYLGFLCTT GI+ HEI NYT++ CN+ M HP N N+PSI +SH
Sbjct: 667 PSAALDPGLIFDAGYEDYLGFLCTTPGISAHEIRNYTNTACNYDMKHPSNFNAPSIAVSH 726

Query: 729 LVGTQTVTRTVTNVAE-EETYVLTARMDPAVAIETDPPAMTLRSGSSRKFTVTLTARSLT 788
           LVGTQTVTR VTNVAE EETY +TARM P++AIE +PPAMTLR G++R F+VT+T RS++
Sbjct: 727 LVGTQTVTRKVTNVAEVEETYTITARMQPSIAIEVNPPAMTLRPGATRTFSVTMTVRSVS 786

Query: 789 GTYSFGEVLMKGSRGHKVRIPVVAMGYQR 817
           G YSFGEV +KGSRGHKVRIPVVA+G++R
Sbjct: 787 GVYSFGEVKLKGSRGHKVRIPVVALGHRR 815

BLAST of MC08g2075 vs. TAIR 10
Match: AT4G30020.1 (PA-domain containing subtilase family protein )

HSP 1 Score: 1331.2 bits (3444), Expect = 0.0e+00
Identity = 655/810 (80.86%), Postives = 728/810 (89.88%), Query Frame = 0

Query: 9   VLVFLALLVVGRADIYIVSIEGEPIVSYRGDLDGFEATAVESDEKIDPTSELVTSYARHL 68
           VLVF    +   A+IYIV++EGEPI+SY+G  +GFEATAVESDEKID TSELVTSYARHL
Sbjct: 7   VLVFFTCFLTVTAEIYIVTMEGEPIISYKGGDNGFEATAVESDEKIDTTSELVTSYARHL 66

Query: 69  ENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAEILRHTPIVKSVERDWKVRRL 128
           E KHDMLLGMLF  GS+KKLYSYKHLINGFA  ++P+QAE+LR  P VKSV+RDWKVR+L
Sbjct: 67  ERKHDMLLGMLFVEGSYKKLYSYKHLINGFAAHVSPDQAEMLRRAPGVKSVDRDWKVRKL 126

Query: 129 TTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTE-PFGPCLKYK 188
           TTHTP+FLGLPT VWPTGGG+DRAGEDIVIGF+DSGI+PHHPSFA+++T  P+GP   YK
Sbjct: 127 TTHTPQFLGLPTDVWPTGGGYDRAGEDIVIGFIDSGIFPHHPSFASHHTTVPYGPHPSYK 186

Query: 189 GKCEVDPNTKKSFCNGKIIGAQHFAEAAKAAGAFNPSIHFASPLDGDGHGSHTAAIAAGN 248
           GKCE DP+TK SFCNGKIIGAQHFAEAAKAAGAFNP I FASP+DGDGHGSHTAAIAAGN
Sbjct: 187 GKCEEDPHTKISFCNGKIIGAQHFAEAAKAAGAFNPDIDFASPMDGDGHGSHTAAIAAGN 246

Query: 249 NGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSV 308
           NGIPVRMHGYEFGKASGMAPRARIAVYKALYR+FGGFVADVVAAIDQAVHDGVDILSLSV
Sbjct: 247 NGIPVRMHGYEFGKASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSV 306

Query: 309 GPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAID 368
           GPNSPPATTK T+LNPFDATLL AVKAGVFVAQAAGNGGPFPKTLVSYSPWI TVAAAID
Sbjct: 307 GPNSPPATTKTTFLNPFDATLLGAVKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAID 366

Query: 369 DRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLIDSSVTKYSPSDCQRPEVLN 428
           DRRYKNHLTLGNGK+LAG+GLSP+T  +R+Y +V+ANDVL+ SS  KY+PSDCQ+PEVLN
Sbjct: 367 DRRYKNHLTLGNGKMLAGIGLSPSTRPHRSYKMVSANDVLLGSSGMKYNPSDCQKPEVLN 426

Query: 429 KRLVEGKVLLCGYSFSFVVGTASVKKVSQTAKALGAAGFVLAVENVSPGAKFDPVPVGIP 488
           K+LVEG +LLCGYSF+FV G+AS+KKV++TAK LGAAGFVL VENVSPG KFDPVP  IP
Sbjct: 427 KKLVEGNILLCGYSFNFVAGSASIKKVAETAKHLGAAGFVLVVENVSPGTKFDPVPSCIP 486

Query: 489 GILITDVSKSMDLIDYYNTSTPRDWTGRVKSFEAVGRIGDGLMPLLYKSAPEVALFSARG 548
           GILITDVSKSMDLIDYYN +T RDW GRVK F+A G IGDGL P+L+KSAPEVALFSARG
Sbjct: 487 GILITDVSKSMDLIDYYNVTTSRDWMGRVKDFKAEGSIGDGLEPILHKSAPEVALFSARG 546

Query: 549 PNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIAGI 608
           PN +DFSFQDADLLKPDILAPGSLIW+AWS NGTDE NY+GEGFA+ISGTSMAAPHIAGI
Sbjct: 547 PNTKDFSFQDADLLKPDILAPGSLIWSAWSANGTDEANYIGEGFALISGTSMAAPHIAGI 606

Query: 609 AALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQCSETEAMKLVPATPFDYGSGAV 668
           AALVKQKHP WSPAAIKSALMTTST +DRAGRPL+AQQ SETE + LV ATPFDYGSG V
Sbjct: 607 AALVKQKHPQWSPAAIKSALMTTSTVIDRAGRPLQAQQYSETETVTLVKATPFDYGSGHV 666

Query: 669 NPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTSSPCNFTMGHPWNLNSPSITIS 728
           NP AALDPGLIFDAGYEDY+GFLCTT GI+ HEI N+T++PCNF M HP N N+PSI IS
Sbjct: 667 NPSAALDPGLIFDAGYEDYIGFLCTTPGIDAHEIKNFTNTPCNFKMVHPSNFNTPSIAIS 726

Query: 729 HLVGTQTVTRTVTNVA-EEETYVLTARMDPAVAIETDPPAMTLRSGSSRKFTVTLTARSL 788
           HLV TQTVTR VTNVA EEETY +T+RM+PA+AIE  PPAMT+R+G+SR F+VTLT RS+
Sbjct: 727 HLVRTQTVTRRVTNVAEEEETYTITSRMEPAIAIEVSPPAMTVRAGASRTFSVTLTVRSV 786

Query: 789 TGTYSFGEVLMKGSRGHKVRIPVVAMGYQR 817
           TG YSFG+V +KGSRGHKV +PVVAMG +R
Sbjct: 787 TGAYSFGQVTLKGSRGHKVTLPVVAMGQRR 816

BLAST of MC08g2075 vs. TAIR 10
Match: AT5G44530.1 (Subtilase family protein )

HSP 1 Score: 653.3 bits (1684), Expect = 2.6e-187
Identity = 368/805 (45.71%), Postives = 499/805 (61.99%), Query Frame = 0

Query: 23  IYIVSIEGEPIVSYRGDLDGFEATAVESDEKIDPTSELVTSYARHLENK-------HDML 82
           +YIV+++  PIV    + +  +    +   K+ P +    S  RH ++K       HD  
Sbjct: 38  VYIVTLKQPPIVHLFEEQE-LKHKKSKFTPKLRPRN---NSRKRHGKSKIPSVVQSHDSF 97

Query: 83  LGMLFERGSFKKLYSYKHLINGFAVDITPEQAEILRHTPIVKSVERDWKVRRLTTHTPEF 142
           L    +   + KLYSY +LINGFA+ I  +QAE L     V ++  D+ VR  TT+TP+F
Sbjct: 98  LRKTLKGEKYIKLYSYHYLINGFALFINSQQAEKLSMRKEVANIVLDYSVRTATTYTPQF 157

Query: 143 LGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPFGPCLK-YKGKCEVDP 202
           +GLP G W   GGF+ AGE ++IGF+D+GI P+HPSF   +++   P  K + G CEV P
Sbjct: 158 MGLPQGAWVKEGGFEIAGEGVIIGFIDTGIDPNHPSFNDNDSKRSYPIPKHFSGVCEVTP 217

Query: 203 NTKKSFCNGKIIGAQHFAEAAKAAGAFNPSIHFASPLDGDGHGSHTAAIAAGNNGIPVRM 262
           +     CN K+IGA+HFA++A   G FN S  +ASP DGDGHG+HTA++AAGN+G+PV +
Sbjct: 218 DFPSGSCNKKLIGARHFAQSAVTRGIFNSSEDYASPFDGDGHGTHTASVAAGNHGVPVIV 277

Query: 263 HGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPPA 322
             + FG ASG+APRA I+VYKALY+ FGGF ADVVAAIDQA  DGVDILSLS+ PN  P 
Sbjct: 278 SNHNFGYASGIAPRAFISVYKALYKSFGGFAADVVAAIDQAAQDGVDILSLSITPNRKPP 337

Query: 323 TTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAIDDRRYKNH 382
               T+ NP D  LLSAVKAG+FV QAAGN GP PKT+ S+SPWI TV A+  DR Y N 
Sbjct: 338 GV-ATFFNPIDMALLSAVKAGIFVVQAAGNTGPAPKTMSSFSPWIFTVGASSHDRVYSNS 397

Query: 383 LTLGNGKILAGLGLSPATHLNRTYTLVAANDVLIDS-SVTK-YSPSDCQRPEVLNKRLVE 442
           LTLGN   + G+G +  T   + Y +++A   L +S SV K     +CQ  E  ++  V 
Sbjct: 398 LTLGNNVTIPGMGFAIPTDSGKMYKMISAFHALNNSTSVDKDMYVGECQDYENFDQDRVS 457

Query: 443 GKVLLCGYSFSFVVGTASVKKVSQTAKALGAAGFVLAVENVSPGAKFDPVPVGIPGILIT 502
           GK+L+C YS  FV+G +++K+    AK L A G +  ++    G + +P P+ +PGI+I 
Sbjct: 458 GKLLICSYSARFVLGLSTIKQALDVAKNLSATGVIFYIDPYVLGFEINPTPMDMPGIIIP 517

Query: 503 DVSKSMDLIDYYNTSTPRDWTGR-VKSFEAVGRIGDGLMPLLYKSAPEVALFSARGPNIR 562
            V  S  L+ YYN+S  RD T + + SF AV  I  GL       AP+V  +SARGP+  
Sbjct: 518 SVEDSKTLLKYYNSSIQRDVTTKEIVSFGAVAAIEGGLNANFSNRAPKVMYYSARGPDPE 577

Query: 563 DFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIAGIAALV 622
           D SF DAD+LKP+++APG+ IW AWS   TD   + GE FAM+SGTSMAAPH+AG+AAL+
Sbjct: 578 DNSFNDADVLKPNLVAPGNSIWGAWSSASTDSTEFEGEKFAMMSGTSMAAPHVAGVAALI 637

Query: 623 KQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQCSETEAMKLVPATPFDYGSGAVNPRA 682
           KQ +P ++P+ I SAL TT+   D  G P+ AQ+        L  ATP D GSG VN  A
Sbjct: 638 KQSYPQFTPSTISSALSTTALLNDNKGSPIMAQRTYSNPDQSLYTATPSDMGSGFVNATA 697

Query: 683 ALDPGLIFDAGYEDYLGFLCTTAGINVHE--IHNYTS--SPCNFTMGHPWNLNSPSITIS 742
           ALDPGL+FD  +EDY+ FLC   GIN  +  + NYT    P N T    ++LN PSIT+S
Sbjct: 698 ALDPGLVFDTSFEDYISFLC---GINGSDTVVFNYTGFRCPANNTPVSGFDLNLPSITVS 757

Query: 743 HLVGTQTVTRTVTNVAEEETYVLTARMDPAVAIETDPPAMTLRSGSSRKFTVTLTARSLT 802
            L GTQT  R++ N+A  ETY +       V+++  P   ++  G ++  +VTLT    +
Sbjct: 758 TLSGTQTFQRSMRNIAGNETYNVGWSPPYGVSMKVSPTQFSIAMGENQVLSVTLTVTKNS 817

Query: 803 GTYSFGEVLMKGSRGHKVRIPVVAM 813
            + SFG + + G+ GH V IPV  +
Sbjct: 818 SSSSFGRIGLFGNTGHIVNIPVTVI 834

BLAST of MC08g2075 vs. TAIR 10
Match: AT4G20430.1 (Subtilase family protein )

HSP 1 Score: 643.3 bits (1658), Expect = 2.7e-184
Identity = 350/747 (46.85%), Postives = 468/747 (62.65%), Query Frame = 0

Query: 72  HDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAEILRHTPIVKSVERDWKVRRLTTH 131
           HD LL    +   + KLYS+ +LINGFAV ++ +QAE L     V ++  D+ VR  TT+
Sbjct: 103 HDSLLRNALKGEKYIKLYSFHYLINGFAVFVSSQQAETLSRRREVANIVLDFSVRTATTY 162

Query: 132 TPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNT--EPFGPCLKYKGK 191
           TP+F+GLP G W   GG++ AGE IVIGF+D+GI P HPSF   +T    +     + G 
Sbjct: 163 TPQFMGLPKGAWVKEGGYETAGEGIVIGFIDTGIDPTHPSFNGTDTSQRQYPIPNHFSGV 222

Query: 192 CEVDPNTKKSFCNGKIIGAQHFAEAAKAAGAFNPSIHFASPLDGDGHGSHTAAIAAGNNG 251
           CEV P+     CN K++GA+HFA++A   G FN S  +ASP DGDGHG+HTA+IAAGN+G
Sbjct: 223 CEVTPDFPSGSCNRKLVGARHFAQSAITRGIFNSSEDYASPFDGDGHGTHTASIAAGNHG 282

Query: 252 IPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSVGP 311
           +   + G+ FG ASG+APRA I+VYKALY+ FGGF ADVVAAIDQA  DGVDILSLS+ P
Sbjct: 283 VSAVVSGHNFGSASGIAPRAHISVYKALYKSFGGFAADVVAAIDQAAQDGVDILSLSITP 342

Query: 312 NSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAIDDR 371
           N  P     T+ NP D  +LSAVKAG+FV QAAGN GP PK++ S+SPWI TV AA  DR
Sbjct: 343 NRRPPGV-ATFFNPLDMAMLSAVKAGIFVVQAAGNTGPSPKSMSSFSPWIFTVGAASHDR 402

Query: 372 RYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLIDSSVT---KYSPSDCQRPEVL 431
            Y N + LGN   + G+GL+  T   + YT+++A D L + S          +CQ     
Sbjct: 403 DYSNSIVLGNNVSIPGVGLALRTDEGKKYTMISALDALKNKSSVVDKDMYVGECQDYGSF 462

Query: 432 NKRLVEGKVLLCGYSFSFVVGTASVKKVSQTAKALGAAGFVLAVENVSPGAKFDPVPVGI 491
           +K ++ G +L+C YS  FV+G +++K+    AK L A G V  ++    G + +P P+ +
Sbjct: 463 DKDVIRGNLLICSYSIRFVLGLSTIKQALAVAKNLSAKGVVFYMDPYVLGFQINPTPMDM 522

Query: 492 PGILITDVSKSMDLIDYYNTSTPRDWTGR-VKSFEAVGRIGDGLMPLLYKSAPEVALFSA 551
           PGI+I     S  L+ YYN+S  RD T + +  F AV  I  G        AP++  +SA
Sbjct: 523 PGIIIPSAEDSKVLLKYYNSSLVRDGTTKEIVRFGAVAAIAGGQNANFSNRAPKIMYYSA 582

Query: 552 RGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIA 611
           RGP+ +D  F DAD+LKP+++APG+ IW AWS   T+   + GE FAM+SGTSMAAPH+A
Sbjct: 583 RGPDPQDSLFNDADILKPNLVAPGNSIWGAWSSAATESTEFEGESFAMMSGTSMAAPHVA 642

Query: 612 GIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQCSETEAMKLVPATPFDYGSG 671
           G+AALVKQK   +SP+AI SAL TTS   D  G  + AQ+        + PATPFD G+G
Sbjct: 643 GVAALVKQKFRKFSPSAIASALSTTSVLFDNKGEAIMAQRAYANPDQTISPATPFDMGNG 702

Query: 672 AVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTSSPC---NFTMGHPWNLNSP 731
            VN  AALDPGLIFD  +EDY+ FLC   G +   + NYT + C   N T+    +LN P
Sbjct: 703 FVNATAALDPGLIFDTSFEDYMSFLCGING-SAPVVFNYTGTNCLRNNATISGS-DLNLP 762

Query: 732 SITISHLVGTQTVTRTVTNVAEEETYVLTARMDPAVAIETDPPAMTLRSGSSRKFTVTLT 791
           SIT+S L  T+TV R +TN+A  ETY ++      V I   P   ++ SG ++  +V LT
Sbjct: 763 SITVSKLNNTRTVQRLMTNIAGNETYTVSLITPFDVLINVSPTQFSIASGETKLLSVILT 822

Query: 792 ARSLTGTYSFGEVLMKGSRGHKVRIPV 810
           A+  +   SFG + + G+ GH VRIPV
Sbjct: 823 AKRNSSISSFGGIKLLGNAGHIVRIPV 846

BLAST of MC08g2075 vs. TAIR 10
Match: AT1G30600.1 (Subtilase family protein )

HSP 1 Score: 636.7 bits (1641), Expect = 2.5e-182
Identity = 359/810 (44.32%), Postives = 499/810 (61.60%), Query Frame = 0

Query: 23  IYIVSIEGEPIVSYRGDLDGFEATAVESDEKIDPTSELV---TSYARHLENKHDMLLGML 82
           +YIV+++  P V + G        + +S   +  TS  +    + +  +   HD LL  +
Sbjct: 36  VYIVTLKDRPSVHFSG------RESSDSKHSLTATSSQIYRTLNRSASIIRVHDSLLRNV 95

Query: 83  FERGSFKKLYSYKHLINGFAVDITPEQAEILRHTPIVKSVERDWKVRRLTTHTPEFLGLP 142
             + ++ KLYSY +LINGF+  +T +QA+ L     V++V  D+ V + TTHTP+FLGLP
Sbjct: 96  LRKENYLKLYSYHYLINGFSAVLTRKQADRLAAREEVENVVLDFLVEKATTHTPQFLGLP 155

Query: 143 TGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFA------TYNTEPFGPCLKYKGKCEVD 202
            G W   GG + AGE +VIGF+D+GI P HPSF+      TY+  P      + G CEV 
Sbjct: 156 RGAWLRDGGSEYAGEGVVIGFIDTGIDPTHPSFSDKISGHTYSVPP-----HFTGVCEVT 215

Query: 203 PNTKKSFCNGKIIGAQHFAEAAKAAGAFNPSIHFASPLDGDGHGSHTAAIAAGNNGIPVR 262
                  CN K+IGA+HFAE+A + G  N S   ASP DG+GHG+HTA++AAGN+GIPV 
Sbjct: 216 IGFPPGSCNRKLIGARHFAESALSRGVLNSSQDDASPFDGEGHGTHTASVAAGNHGIPVV 275

Query: 263 MHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPP 322
           + G+  G ASGMAPRA IA+YKALY+ FGGF AD++AAIDQA  DGVDI++LS+ PN  P
Sbjct: 276 VAGHRLGNASGMAPRAHIAIYKALYKRFGGFAADIIAAIDQAAQDGVDIINLSITPNRRP 335

Query: 323 ATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAIDDRRYKN 382
                T+ NP D  LLSAVKAG+FV QAAGN GP PK++ S+SPWI TV A   DR Y N
Sbjct: 336 PGI-ATFFNPIDMALLSAVKAGIFVVQAAGNTGPAPKSMSSFSPWIFTVGATSHDRVYSN 395

Query: 383 HLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLIDSSVTKYS--PSDCQRPEVLNKRLV 442
            + LGN   + G+GL+  T +   + LV A   L + +    +    +CQ     +++LV
Sbjct: 396 SIILGNNVTIPGVGLASGTRI--MHKLVLATHALRNGTTVMDAIYVGECQDSSSFDQKLV 455

Query: 443 EGKVLLCGYSFSFVVGTASVKKVSQTAKALGAAGFVLAVENVSPGAKFDPVPVGIPGILI 502
           +GK+L+C Y+  F++G +++K+   TAK L AAG V  ++  + G +    P+ IPGILI
Sbjct: 456 QGKILVCSYTVRFILGVSTIKQALLTAKNLTAAGLVFYIDPSATGFQMTSSPMDIPGILI 515

Query: 503 TDVSKSMDLIDYYNTSTPRD-WTGRVKSFEAVGRIGDGLMPLLYKSAPEVALFSARGPNI 562
           +    S  L+ YYN+S  R+  +G++    +V +I  G+ P    +AP+V  FSARGP+ 
Sbjct: 516 SSPQDSQALLRYYNSSLLRENGSGKIVGSASVAKIVGGMRPTYGITAPKVMYFSARGPDP 575

Query: 563 RDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIAGIAAL 622
            D SF DAD++KP+++APG+ IW AWSP G    ++ GE FAM SGTSM+APH+ GIAAL
Sbjct: 576 EDDSFVDADIMKPNLVAPGNAIWGAWSPLGIGTNDFQGERFAMESGTSMSAPHVTGIAAL 635

Query: 623 VKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQCSETEAMKLVPATPFDYGSGAVNPR 682
           +KQK P+++PAAI SAL TT++  DR G  + AQ+      +   PATPFD GSG VN  
Sbjct: 636 IKQKFPHFTPAAIASALSTTASLSDRKGEHIMAQRTVLNPDISQSPATPFDMGSGFVNAT 695

Query: 683 AALDPGLIFDAGYEDYLGFLCTTAGINVHE--IHNYTSSPC---NFTMGHPWNLNSPSIT 742
           AALDPGLIFD GY +Y+ FLC   GIN     + NYT   C   N ++    +LN PS+T
Sbjct: 696 AALDPGLIFDIGYNEYMKFLC---GINGSSPVVLNYTGESCSSYNSSLAAS-DLNLPSVT 755

Query: 743 ISHLVGTQTVTRTVTNV---AEEETYVLTARMDPAVAIETDPPAMTLRSGSSRKFTVTLT 802
           I+ LVGT+ V R VTN+   A  ETY++      +V+++  P   T+ +G +R  ++   
Sbjct: 756 IAKLVGTRAVLRWVTNIATTATNETYIVGWMAPDSVSVKVSPAKFTIGNGQTRVLSLVFR 815

Query: 803 ARSLTGTYSFGEVLMKGSRGHKVRIPVVAM 813
           A       SFG + + G RGH V IPV  +
Sbjct: 816 AMKNVSMASFGRIGLFGDRGHVVNIPVAVI 827