MC08g1931 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g1931
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionRING/FYVE/PHD zinc finger superfamily protein
LocationMC08: 27491000 .. 27495485 (-)
RNA-Seq ExpressionMC08g1931
SyntenyMC08g1931
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GGCGAAGGGAAGGGACAAAAGAGAGAAAAGTTTCTAATTTAAATTGGTAACGTAGATGATTGACGGCCGTCGGCCACGGGGAATGGCACGTGAATTGACGCAATTACCCTTGGTGGCCGTTGGATTGGACTGTACGCAAAGACGTGGAAGTTTCGGGCGAAGAAAACTCCGCTTCTTCTCCCCTATATATAGCGAACCAGGTCCACCCTGCTCCAAATTCTTCTTATTTTTAGTCTTTTGCGGAATCTTTGTACCACTCGCGGACCCATCTTCAAATTCATTTCTACTCTCGCAAAATCTTCTTTCTCATTCCGCACCTAACGCGCTTTCTCTTCTTCTCCCAGGTACGATTTCCCTTTTTCTTTCTCTCAATTTCACAATTTTTTTCATCTCAATCTACTTTTCCTTGTTTTGTCCCCTCCAATTTTGATAATTTCTTCCCGTAATTGATCGAGTTGTCCTCGTAGGGTTTCTTTTTTCTGGGAATTCACCGTGTGGGTTACTTTTTCATGATATAGGGGTTTCTGTGCGGTTTATAGGACAATGAGCGACCATCTCGTCCTGTATGTTGATCATTTGATTCGACCAGTCGCTCCAGTTGAACCTGTGTCCGAGCCGGCCGACCCTGTTCCTTCTGCACCGCGTGAAGCTGTTGTGGACTCAGCTGCGGCCGGTCCTTCGTGTTCGACTGCAACTGCGGTTGCGGATGATCGGACGGTGAAGCAGCACGCACCCAATGAGGAGGAGCCTCTGATTCAGGCCGCTGAGTGCCGCATTTGTCAGGAAGAAGACAGCCTCAATAATTTAGAAACTCCCTGCGCATGTAGTGGTAGTCTTAAGGTATTACCCCTTTTTTATTATTTGTTAAATTTGAATTTGAATAATCAGGACATGGAAGCCCCTTTGAATTCGGTCCAGTCTTTTTCACTGCGTTGTATTTGTGTTTGTGCTGAATTGGAAACTTTGGAACTAGTTCTTTCGCAAGAATCTGCTTCGGTGTACAGTATTGGTTAAATTGAGTTTAGTACTAATATGCTTTTTCCTGTCTTGTGGATGGGTTTAAGTCTGTATTAATTGCTTTGATTTCTTTTTCCTTTTAATTTCTTCTTACGTTCAATGTGATATAGTTTTCATAGAAGCAAGCTGGAAAATGATATAAAATAAAATCTTGAGAGGTTTTATATCAGTAAGGTGGGGAGGACTGGGTTGAAGTACATGAAATAATTTGGTTGTTTGGTAATTAATAGTTTTATTCTTAGCTTTGTTAGTTGAATATTTTCTATAACTTTTTCCCCAATGCTATGATCGACAGGAACTTTTGTGTGCTGGTATGTGGCATATTATCTGAGTTATAAAGTTGCTTGATAAAACTTGACAAGTATGGCTATCATGCATGGGGTGTCGTGGTAGAATTATAGTGATCCATGACTCTTATTTTTTTTCTTCATAATGTAACTAAATCAGGTTAGGTTCTTTGAATTGACCAATATCAATACCCATGGTGAAATTTGTCGGGTTTTTTTGTTTATTAATTTTTTGCATGTGTCTGAATGTTTTTCTGTTCAGTATGCTCACAGAAAGTGCGTTCAACATTGGTGCAATGAGAAAGGGGACATAACTTGTGAGATATGTCATCAGGTATGCACGATGATTTTGATTTGATCTCATATCATTTTAGCCTTATTTTTAATTTTTAATCTTGCATTTCTAGAATTTCAAATAAATTGGTAATTCCTTATACTTCAGCTTGCGAGAAAGTCTTCCTACATTGTTTTTGAACTCATAAGTCTTTGAATTTTAAAAAATTGCAGGATTTACACTTGGAGTTAAAAATTTTCCATTATTACTGTTTTATAAAGTGCATGTTTAAGTACGCTTTGCACTTAAGTGGAAGAGGGAGACCATGCCTAGTGAAGGCGAGGCAACCTCTATTAGCGTTTTCATTTTATGTCTAAAAAATGTATCTTCCACCCCACCCAGGGGACTTGCGAATGAGAGGGAGGAAAACCGGAAAACCATACAATTCAGACGTTTGTATGGGTGGGTTGTTGTTGTAGGTGAGGGAGTAGTTGGAGAAAACCATACAGTTGAGAAATAGAATTTGCTACTCTAGAAACCGTCTGAATGAAGGAGACTTTCGGCTTTCTTTTTATTCTCTTTCTTTTTCTGTCTCTCTCAATCTCGACCATCTCTTTGTTTATTCTCTCCGACTCCTACCTTTTCCCTTCTTTTCCCTTGATATGTCCATATCTGAAAAGCCATAATAGTGGTTCACTATAGGGCATTCAAAAGGATTTGGGTCTTTTGGCAATGGGCTGGATTCGTTTTTTGGGCAGGTTGAATCTGGCGTCAAGGAAGATTGTTCCAACATGAGAGGAGAAGATGAGCAGCATGTCTCAATAGGAGGGTTCCCCCTTTTCTCACGTTCAACCAAAATTAACGTGGATACTGCTTGGGATGTGCTATGAGTGGAGAAGCTAGTTGGGGCATTATTGTCAGAGATGATAGAGGTGAGGAGTGGCTTCCAAGAAGATGCAGGTTATTTATTGTTAGAGTGTAGTGATTGATGAGACATTTGGCTGACTAGATAAGCGTTGGTGTTTGGGGCTTAAGAGATATAGTTGTTGAAACTTACTGTTTGTTGGTGAAATCTTGATCCTGTAGCCGTTCCATTGCATAGTCCGGACCTTTTCAGAAATGGATTGTTTGTAGGACTTCCCGCCCTTGTAGTTGGTTGGATGCCCTCTCTATTTGCACGGTGCATCAGTTAGACTATGAATGCTTCTTTTTTCCAATTAAATAAAATGAAATGTTTTTTTTTCTGAACCAAAATGCCATACTCTCTGTCTGTACTTGTTATTGAATGGTAGGATGAGATGGTGTGCATTCACAGCATCTTTTGTGACGATGTTTTCTTGTTTTAATTCCTCATTGATGTCGGTATGTTGTATTCAATTCCCAGTCTTATCAACCTGGATATACTGCACCACCTCGTACTGAAGAAACTGCTATTGATATGGAGTAAGTTTAATTTTTCTTTTGGTAATATCTAATTGTTCCGTGAATAGTATTATTGATAAGCTAGTTTCTGAACACCTGCAAGGGTTTTTTTCTTCATGTAATTTCTGTATTCATATTTGAAATATTGATCTGGATGTGGATATGCACAGGGGAAGATGGACCATCTCTGGTAATCCGTTAGACTTGAGAGATACTCGGCTATTGGCCATTGCAGAGGCAGAACGGCATTTCTTGGAAGCTGAATATGACGATTATGCTACTTCAAATGACAGTGGAGCTGCATTTTGCCGATCAGCTGCTCTTATTGTAGGTTTTCTAATACTCGTAGAAATAAAAAAGAAATTGCACCTTCTATCCTCTAGAATTTTCCATTCAGCTGTATCTGATGTTTCTATTTGATCTAACATGGCAGTTACTAGCCCTTCTCCTTCTGCGGCATGCATTGACCATAACAGATCCCGATGGAGAAGATGATGCATCTGCCTTTTTTTCTGTAAGTGGATATTGAGTTTATCTTCTTAAATTTGAAAGGAAAAATAGTGTGTATGATGGAGGATGGGTTTATAATCTGGATGGTACCTGTGACAGATTTTCTTGCTTCGTGCTGCTGGTTTTCTCTTACCCTGCTACATCATGGCCTGGGCCATCAGTATCTTGCAGCGTCGAAGACAAAGACAGGTTGGTGATTTGGGAGTCGTTTCTATTTTCAAAATTTTGTTCTTAAAAACTATTTTGCAGACTAATTTTTCTAAACTGTTCTGTTTAAATTTTAAGAATGGTTTGTGTGGGGTGTGAAACTACAGGAGGCGGCAGCACTGGCTGCAACACAAGTAGCGTTTGTACTACAACATGGGCAGCACAGAGGTTTACAATTTGCGATTGCATCATCAGGACCACCTCTCACCCCTCACCCACATCCACACCCTCACCCTACTGTTTGAACCGCAGCCCGCCCCAACCGTGTAATATTATATATTATATTTTCGAACCAACCTTCCTTCTACTACTTCCACTGGTCATCATCATCATTTTTATGTACAAACAATTTCCTCAACTGCAATTCTTCGCTGCCTGTAATCTCTGAATCTTTCCATCTTCCAAAACCAGGTACCCTGCTAACAAACTTCATTGTTTTTTACGTGCCCTTCTACTCCACGTGATTGCTTGATTGATGCTTCTTCTTGTGCTTATGGAAAACAAGGAAGAGATTTAGGGGGCAAACATAGGTTTTCCTCTAAAGAATTTGCTATTTCTTGTACAATCTGTAGGGTATCTCAAGAATTTGGTATTGCATATGAAAGAACAGAATGGTAAGCAAAACAGTAGAGAGCGTAATAAGATCTTAGTTTTGGACAAGATCGAGTCAATTTTGGAGAGAGGAAAAAGGTTAATGATTTGATCTTTATTCCATTGTTCTTGCCTCGACTCCTGTATTATCATACTGCTAAATGATATGTGTGCGATG

mRNA sequence

GGCGAAGGGAAGGGACAAAAGAGAGAAAAGTTTCTAATTTAAATTGGTAACGTAGATGATTGACGGCCGTCGGCCACGGGGAATGGCACGTGAATTGACGCAATTACCCTTGGTGGCCGTTGGATTGGACTGTACGCAAAGACGTGGAAGTTTCGGGCGAAGAAAACTCCGCTTCTTCTCCCCTATATATAGCGAACCAGGTCCACCCTGCTCCAAATTCTTCTTATTTTTAGTCTTTTGCGGAATCTTTGTACCACTCGCGGACCCATCTTCAAATTCATTTCTACTCTCGCAAAATCTTCTTTCTCATTCCGCACCTAACGCGCTTTCTCTTCTTCTCCCAGGGGTTTCTGTGCGGTTTATAGGACAATGAGCGACCATCTCGTCCTGTATGTTGATCATTTGATTCGACCAGTCGCTCCAGTTGAACCTGTGTCCGAGCCGGCCGACCCTGTTCCTTCTGCACCGCGTGAAGCTGTTGTGGACTCAGCTGCGGCCGGTCCTTCGTGTTCGACTGCAACTGCGGTTGCGGATGATCGGACGGTGAAGCAGCACGCACCCAATGAGGAGGAGCCTCTGATTCAGGCCGCTGAGTGCCGCATTTGTCAGGAAGAAGACAGCCTCAATAATTTAGAAACTCCCTGCGCATGTAGTGGTAGTCTTAAGTATGCTCACAGAAAGTGCGTTCAACATTGGTGCAATGAGAAAGGGGACATAACTTGTGAGATATGTCATCAGTCTTATCAACCTGGATATACTGCACCACCTCGTACTGAAGAAACTGCTATTGATATGGAGGGAAGATGGACCATCTCTGGTAATCCGTTAGACTTGAGAGATACTCGGCTATTGGCCATTGCAGAGGCAGAACGGCATTTCTTGGAAGCTGAATATGACGATTATGCTACTTCAAATGACAGTGGAGCTGCATTTTGCCGATCAGCTGCTCTTATTGTAGCCCTTCTCCTTCTGCGGCATGCATTGACCATAACAGATCCCGATGGAGAAGATGATGCATCTGCCTTTTTTTCTATTTTCTTGCTTCGTGCTGCTGGTTTTCTCTTACCCTGCTACATCATGGCCTGGGCCATCAGTATCTTGCAGCGTCGAAGACAAAGACAGGAGGCGGCAGCACTGGCTGCAACACAAGTAGCGTTTGTACTACAACATGGGCAGCACAGAGGTTTACAATTTGCGATTGCATCATCAGGACCACCTCTCACCCCTCACCCACATCCACACCCTCACCCTACTGTTTGAACCGCAGCCCGCCCCAACCGTGTAATATTATATATTATATTTTCGAACCAACCTTCCTTCTACTACTTCCACTGGTCATCATCATCATTTTTATGTACAAACAATTTCCTCAACTGCAATTCTTCGCTGCCTGTAATCTCTGAATCTTTCCATCTTCCAAAACCAGGGTATCTCAAGAATTTGGTATTGCATATGAAAGAACAGAATGGTAAGCAAAACAGTAGAGAGCGTAATAAGATCTTAGTTTTGGACAAGATCGAGTCAATTTTGGAGAGAGGAAAAAGGTTAATGATTTGATCTTTATTCCATTGTTCTTGCCTCGACTCCTGTATTATCATACTGCTAAATGATATGTGTGCGATG

Coding sequence (CDS)

ATGAGCGACCATCTCGTCCTGTATGTTGATCATTTGATTCGACCAGTCGCTCCAGTTGAACCTGTGTCCGAGCCGGCCGACCCTGTTCCTTCTGCACCGCGTGAAGCTGTTGTGGACTCAGCTGCGGCCGGTCCTTCGTGTTCGACTGCAACTGCGGTTGCGGATGATCGGACGGTGAAGCAGCACGCACCCAATGAGGAGGAGCCTCTGATTCAGGCCGCTGAGTGCCGCATTTGTCAGGAAGAAGACAGCCTCAATAATTTAGAAACTCCCTGCGCATGTAGTGGTAGTCTTAAGTATGCTCACAGAAAGTGCGTTCAACATTGGTGCAATGAGAAAGGGGACATAACTTGTGAGATATGTCATCAGTCTTATCAACCTGGATATACTGCACCACCTCGTACTGAAGAAACTGCTATTGATATGGAGGGAAGATGGACCATCTCTGGTAATCCGTTAGACTTGAGAGATACTCGGCTATTGGCCATTGCAGAGGCAGAACGGCATTTCTTGGAAGCTGAATATGACGATTATGCTACTTCAAATGACAGTGGAGCTGCATTTTGCCGATCAGCTGCTCTTATTGTAGCCCTTCTCCTTCTGCGGCATGCATTGACCATAACAGATCCCGATGGAGAAGATGATGCATCTGCCTTTTTTTCTATTTTCTTGCTTCGTGCTGCTGGTTTTCTCTTACCCTGCTACATCATGGCCTGGGCCATCAGTATCTTGCAGCGTCGAAGACAAAGACAGGAGGCGGCAGCACTGGCTGCAACACAAGTAGCGTTTGTACTACAACATGGGCAGCACAGAGGTTTACAATTTGCGATTGCATCATCAGGACCACCTCTCACCCCTCACCCACATCCACACCCTCACCCTACTGTTTGA

Protein sequence

MSDHLVLYVDHLIRPVAPVEPVSEPADPVPSAPREAVVDSAAAGPSCSTATAVADDRTVKQHAPNEEEPLIQAAECRICQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICHQSYQPGYTAPPRTEETAIDMEGRWTISGNPLDLRDTRLLAIAEAERHFLEAEYDDYATSNDSGAAFCRSAALIVALLLLRHALTITDPDGEDDASAFFSIFLLRAAGFLLPCYIMAWAISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASSGPPLTPHPHPHPHPTV
Homology
BLAST of MC08g1931 vs. ExPASy Swiss-Prot
Match: A0JN69 (E3 ubiquitin-protein ligase MARCHF3 OS=Bos taurus OX=9913 GN=MARCHF3 PE=2 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 4.2e-07
Identity = 34/104 (32.69%), Postives = 50/104 (48.08%), Query Frame = 0

Query: 19  VEPVSEPADPVPSAPREAVVDSAAAGPSCSTATAVADDRTVKQHAPNEEEPLIQAAECRI 78
           V+ V +    V   P+  +  SA  G   ST       RT+   +P  + P+     CRI
Sbjct: 24  VKTVEDCGSLVNGQPQYVMQVSAKDGQLLSTVV-----RTLATQSPFNDRPM-----CRI 83

Query: 79  CQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH 123
           C E  S  +L +PC C+G+L   HR C++HW +      CE+CH
Sbjct: 84  CHEGSSQEDLLSPCECTGTLGTIHRSCLEHWLSSSNTSYCELCH 117

BLAST of MC08g1931 vs. ExPASy Swiss-Prot
Match: Q86UD3 (E3 ubiquitin-protein ligase MARCHF3 OS=Homo sapiens OX=9606 GN=MARCHF3 PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 4.2e-07
Identity = 34/104 (32.69%), Postives = 50/104 (48.08%), Query Frame = 0

Query: 19  VEPVSEPADPVPSAPREAVVDSAAAGPSCSTATAVADDRTVKQHAPNEEEPLIQAAECRI 78
           V+ V +    V   P+  +  SA  G   ST       RT+   +P  + P+     CRI
Sbjct: 24  VKTVEDCGSLVNGQPQYVMQVSAKDGQLLSTVV-----RTLATQSPFNDRPM-----CRI 83

Query: 79  CQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH 123
           C E  S  +L +PC C+G+L   HR C++HW +      CE+CH
Sbjct: 84  CHEGSSQEDLLSPCECTGTLGTIHRSCLEHWLSSSNTSYCELCH 117

BLAST of MC08g1931 vs. ExPASy Swiss-Prot
Match: Q8BRX9 (E3 ubiquitin-protein ligase MARCHF3 OS=Mus musculus OX=10090 GN=Marchf3 PE=2 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 4.2e-07
Identity = 34/104 (32.69%), Postives = 50/104 (48.08%), Query Frame = 0

Query: 19  VEPVSEPADPVPSAPREAVVDSAAAGPSCSTATAVADDRTVKQHAPNEEEPLIQAAECRI 78
           V+ V +    V   P+  +  SA  G   ST       RT+   +P  + P+     CRI
Sbjct: 24  VKTVEDCGSLVNGQPQYVMQVSAKDGQLLSTVV-----RTLATQSPFNDRPM-----CRI 83

Query: 79  CQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH 123
           C E  S  +L +PC C+G+L   HR C++HW +      CE+CH
Sbjct: 84  CHEGSSQEDLLSPCECTGTLGTIHRSCLEHWLSSSNTSYCELCH 117

BLAST of MC08g1931 vs. ExPASy Swiss-Prot
Match: Q5XIE5 (E3 ubiquitin-protein ligase MARCHF3 OS=Rattus norvegicus OX=10116 GN=Marchf3 PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 4.2e-07
Identity = 34/104 (32.69%), Postives = 50/104 (48.08%), Query Frame = 0

Query: 19  VEPVSEPADPVPSAPREAVVDSAAAGPSCSTATAVADDRTVKQHAPNEEEPLIQAAECRI 78
           V+ V +    V   P+  +  SA  G   ST       RT+   +P  + P+     CRI
Sbjct: 24  VKTVEDCGSLVNGQPQYVMQVSAKDGQLLSTVV-----RTLATQSPFNDRPM-----CRI 83

Query: 79  CQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH 123
           C E  S  +L +PC C+G+L   HR C++HW +      CE+CH
Sbjct: 84  CHEGSSQEDLLSPCECTGTLGTIHRSCLEHWLSSSNTSYCELCH 117

BLAST of MC08g1931 vs. ExPASy Swiss-Prot
Match: Q99M02 (E3 ubiquitin-protein ligase MARCHF2 OS=Mus musculus OX=10090 GN=MARCHF2 PE=2 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 6.1e-06
Identity = 28/100 (28.00%), Postives = 43/100 (43.00%), Query Frame = 0

Query: 37  VVDSAAAGPSCSTATAVADDRTVKQHAPNEEEPLIQAAECRICQEEDSLNNLETPCACSG 96
           VV++   GP    A   + D  +        +       CRIC E  +  NL +PC C+G
Sbjct: 25  VVEATGLGPPQYVAQVTSRDGRLLSTVIRALDSQSDCPFCRICHEGANGENLLSPCGCTG 84

Query: 97  SLKYAHRKCVQHWCNEKGDITCEICHQSYQPGYTAPPRTE 137
           +L   H+ C++ W +      CE+CH  +       P TE
Sbjct: 85  TLGAVHKSCLEKWLSSSNTSYCELCHTEFAVEKRPRPLTE 124

BLAST of MC08g1931 vs. NCBI nr
Match: XP_022144437.1 (uncharacterized protein LOC111014120 [Momordica charantia])

HSP 1 Score: 592 bits (1526), Expect = 2.62e-213
Identity = 295/297 (99.33%), Postives = 296/297 (99.66%), Query Frame = 0

Query: 1   MSDHLVLYVDHLIRPVAPVEPVSEPADPVPSAPREAVVDSAAAGPSCSTATAVADDRTVK 60
           MSDHLVLYVDHLIRPVAPVEPVSEPADPVPSAPREAVVDSAAAGPSCSTATAVADDRTVK
Sbjct: 1   MSDHLVLYVDHLIRPVAPVEPVSEPADPVPSAPREAVVDSAAAGPSCSTATAVADDRTVK 60

Query: 61  QHAPNEEEPLIQAAECRICQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI 120
           QHAPNEEEPLIQAAECRICQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI
Sbjct: 61  QHAPNEEEPLIQAAECRICQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI 120

Query: 121 CHQSYQPGYTAPPRTEETAIDMEGRWTISGNPLDLRDTRLLAIAEAERHFLEAEYDDYAT 180
           CHQSYQPGYTAPPRTEETAIDMEGRWTISGNPLDLRDTRLLAIAEAERHFLEAEYDDYAT
Sbjct: 121 CHQSYQPGYTAPPRTEETAIDMEGRWTISGNPLDLRDTRLLAIAEAERHFLEAEYDDYAT 180

Query: 181 SNDSGAAFCRSAALIV-ALLLLRHALTITDPDGEDDASAFFSIFLLRAAGFLLPCYIMAW 240
           SNDSGAAFCRSAALI+ ALLLLRHALTITDPDGEDDASAFFSIFLLRAAGFLLPCYIMAW
Sbjct: 181 SNDSGAAFCRSAALILLALLLLRHALTITDPDGEDDASAFFSIFLLRAAGFLLPCYIMAW 240

Query: 241 AISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASSGPPLTPHPHPHPHPTV 296
           AISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASSGPPLTPHPHPHPHPTV
Sbjct: 241 AISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASSGPPLTPHPHPHPHPTV 297

BLAST of MC08g1931 vs. NCBI nr
Match: XP_038890663.1 (uncharacterized protein LOC120080164 [Benincasa hispida] >XP_038890664.1 uncharacterized protein LOC120080164 [Benincasa hispida])

HSP 1 Score: 551 bits (1421), Expect = 2.65e-197
Identity = 276/299 (92.31%), Postives = 288/299 (96.32%), Query Frame = 0

Query: 1   MSDHLVLYVDHLIRPVAPVEPVSEPADPVPSAPREAVVDS--AAAGPSCSTATAVADDRT 60
           MSDHLVLYVDHLIRPVAPV+PVS+P DP+PSAPREAV +S  AAAGPSCSTATA  DDRT
Sbjct: 1   MSDHLVLYVDHLIRPVAPVQPVSDPVDPLPSAPREAVANSPAAAAGPSCSTATA--DDRT 60

Query: 61  VKQHAPNEEEPLIQAAECRICQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITC 120
           V+Q APNEEEPLIQAAECRICQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITC
Sbjct: 61  VEQDAPNEEEPLIQAAECRICQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITC 120

Query: 121 EICHQSYQPGYTAPPRTEETAIDMEGRWTISGNPLDLRDTRLLAIAEAERHFLEAEYDDY 180
           EICHQSYQPGYTAPPR+EETAID+EGRWTI+GNP+DLRDTRLLAIAEAERHFLEAEYDDY
Sbjct: 121 EICHQSYQPGYTAPPRSEETAIDIEGRWTIAGNPIDLRDTRLLAIAEAERHFLEAEYDDY 180

Query: 181 ATSNDSGAAFCRSAALIV-ALLLLRHALTITDPDGEDDASAFFSIFLLRAAGFLLPCYIM 240
           A SNDSGAAFCRSAALI+ ALLLLRHALTITDPDG+DDASAFFSIFLLRAAGFLLPCYIM
Sbjct: 181 AASNDSGAAFCRSAALILLALLLLRHALTITDPDGDDDASAFFSIFLLRAAGFLLPCYIM 240

Query: 241 AWAISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASSGPPLTPHPHPHPHPTV 296
           AWAISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIAS+GPPLTPHPHPHPH  V
Sbjct: 241 AWAISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHTAV 297

BLAST of MC08g1931 vs. NCBI nr
Match: XP_022946694.1 (uncharacterized protein LOC111450687 [Cucurbita moschata])

HSP 1 Score: 548 bits (1411), Expect = 3.33e-195
Identity = 273/295 (92.54%), Postives = 284/295 (96.27%), Query Frame = 0

Query: 1   MSDHLVLYVDHLIRPVAPVEPVSEPADPVPSAPREAVVDSAAAGPSCSTATAVADDRTVK 60
           MSDHLVLYVDHLI PVAPV+PVS+P DPVPSAPRE V DSAAA PSCSTATA  DDRTV+
Sbjct: 1   MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSCSTATA--DDRTVE 60

Query: 61  QHAPNEEEPLIQAAECRICQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI 120
           Q APNEE+PLIQAAECRICQEED+LNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI
Sbjct: 61  QDAPNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI 120

Query: 121 CHQSYQPGYTAPPRTEETAIDMEGRWTISGNPLDLRDTRLLAIAEAERHFLEAEYDDYAT 180
           CHQSYQPGYTAPPR+EET IDMEGRWTI+GNPLDLRDTRLLAIAEAER+FLEAEYDDYA 
Sbjct: 121 CHQSYQPGYTAPPRSEETVIDMEGRWTIAGNPLDLRDTRLLAIAEAERNFLEAEYDDYAA 180

Query: 181 SNDSGAAFCRSAALIV-ALLLLRHALTITDPDGEDDASAFFSIFLLRAAGFLLPCYIMAW 240
           SNDSGAAFCRSAALI+ ALLLLRHALTITDPDG+DDASAFFSIFLLRAAGFLLPCYIMAW
Sbjct: 181 SNDSGAAFCRSAALILLALLLLRHALTITDPDGDDDASAFFSIFLLRAAGFLLPCYIMAW 240

Query: 241 AISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASSGPPLTPHPHPHPHP 294
           AISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIAS+GPPLTPHPHPHPHP
Sbjct: 241 AISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHP 293

BLAST of MC08g1931 vs. NCBI nr
Match: XP_023547303.1 (uncharacterized protein LOC111806165 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 541 bits (1395), Expect = 7.88e-193
Identity = 270/295 (91.53%), Postives = 282/295 (95.59%), Query Frame = 0

Query: 1   MSDHLVLYVDHLIRPVAPVEPVSEPADPVPSAPREAVVDSAAAGPSCSTATAVADDRTVK 60
           MSDHLVLYVDHLI PVAPV+PVS+P DP+PSAPRE V DSAAA PS STATA  DDRTV+
Sbjct: 1   MSDHLVLYVDHLIPPVAPVQPVSDPVDPIPSAPREGVSDSAAAIPSSSTATA--DDRTVE 60

Query: 61  QHAPNEEEPLIQAAECRICQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI 120
           Q APNEE+PLIQAAECRICQEED+LNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI
Sbjct: 61  QDAPNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI 120

Query: 121 CHQSYQPGYTAPPRTEETAIDMEGRWTISGNPLDLRDTRLLAIAEAERHFLEAEYDDYAT 180
           CHQSYQPGYTAPPR+EET IDMEGRWTI+GNPLDLRDTRLLAIAEAER+FLEAEYDDYA 
Sbjct: 121 CHQSYQPGYTAPPRSEETVIDMEGRWTIAGNPLDLRDTRLLAIAEAERNFLEAEYDDYAA 180

Query: 181 SNDSGAAFCRSAALIV-ALLLLRHALTITDPDGEDDASAFFSIFLLRAAGFLLPCYIMAW 240
           SNDSGAAFCRSAALI+ ALLLLRHALTITDPDG+DDASAFFSIFLLRAAGFLLPCYIMAW
Sbjct: 181 SNDSGAAFCRSAALILLALLLLRHALTITDPDGDDDASAFFSIFLLRAAGFLLPCYIMAW 240

Query: 241 AISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASSGPPLTPHPHPHPHP 294
           AISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIAS+GPPL PHPHPHPHP
Sbjct: 241 AISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLAPHPHPHPHP 293

BLAST of MC08g1931 vs. NCBI nr
Match: XP_022972853.1 (uncharacterized protein LOC111471364 isoform X2 [Cucurbita maxima])

HSP 1 Score: 533 bits (1372), Expect = 1.01e-189
Identity = 269/296 (90.88%), Postives = 282/296 (95.27%), Query Frame = 0

Query: 1   MSDHLVLYVDHLIRPVAPVEPVSEPADPVPSAPREAVVDSAAAGPSCSTATAVADDRTVK 60
           MSDHLVLYVDHLI PVAPV+PVS+P DPVPSAPRE V DSAAA PS STATA  DDRTV+
Sbjct: 1   MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSSSTATA--DDRTVE 60

Query: 61  QHAPNEEEPLIQAAECRICQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI 120
           Q APNEE+PLIQAAECRICQEED+LNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI
Sbjct: 61  QDAPNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI 120

Query: 121 CHQSYQPGYTAPPRTEETAIDMEGRWTISGNPLDLRDTRLLAIAEAERHFLEAEYDDYAT 180
           CHQSYQPGY+AP R+EET IDMEGRWTI+GNPLDLRDTRLLAIAEAER+FLEAEYDDYA 
Sbjct: 121 CHQSYQPGYSAPLRSEETVIDMEGRWTIAGNPLDLRDTRLLAIAEAERNFLEAEYDDYAA 180

Query: 181 SNDSGAAFCRSAALIV-ALLLLRHALTITDPDGEDD-ASAFFSIFLLRAAGFLLPCYIMA 240
           SNDSGAAFCRSAALI+ ALLLLRHALTITDPDG+DD A+AFFSIFLLRAAGFLLPCYIMA
Sbjct: 181 SNDSGAAFCRSAALILLALLLLRHALTITDPDGDDDDAAAFFSIFLLRAAGFLLPCYIMA 240

Query: 241 WAISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASSGPPLTPHPHPHPHP 294
           WAISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIAS+GPPLTPHPHPHPHP
Sbjct: 241 WAISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHP 294

BLAST of MC08g1931 vs. ExPASy TrEMBL
Match: A0A6J1CTP2 (uncharacterized protein LOC111014120 OS=Momordica charantia OX=3673 GN=LOC111014120 PE=4 SV=1)

HSP 1 Score: 592 bits (1526), Expect = 1.27e-213
Identity = 295/297 (99.33%), Postives = 296/297 (99.66%), Query Frame = 0

Query: 1   MSDHLVLYVDHLIRPVAPVEPVSEPADPVPSAPREAVVDSAAAGPSCSTATAVADDRTVK 60
           MSDHLVLYVDHLIRPVAPVEPVSEPADPVPSAPREAVVDSAAAGPSCSTATAVADDRTVK
Sbjct: 1   MSDHLVLYVDHLIRPVAPVEPVSEPADPVPSAPREAVVDSAAAGPSCSTATAVADDRTVK 60

Query: 61  QHAPNEEEPLIQAAECRICQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI 120
           QHAPNEEEPLIQAAECRICQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI
Sbjct: 61  QHAPNEEEPLIQAAECRICQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI 120

Query: 121 CHQSYQPGYTAPPRTEETAIDMEGRWTISGNPLDLRDTRLLAIAEAERHFLEAEYDDYAT 180
           CHQSYQPGYTAPPRTEETAIDMEGRWTISGNPLDLRDTRLLAIAEAERHFLEAEYDDYAT
Sbjct: 121 CHQSYQPGYTAPPRTEETAIDMEGRWTISGNPLDLRDTRLLAIAEAERHFLEAEYDDYAT 180

Query: 181 SNDSGAAFCRSAALIV-ALLLLRHALTITDPDGEDDASAFFSIFLLRAAGFLLPCYIMAW 240
           SNDSGAAFCRSAALI+ ALLLLRHALTITDPDGEDDASAFFSIFLLRAAGFLLPCYIMAW
Sbjct: 181 SNDSGAAFCRSAALILLALLLLRHALTITDPDGEDDASAFFSIFLLRAAGFLLPCYIMAW 240

Query: 241 AISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASSGPPLTPHPHPHPHPTV 296
           AISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASSGPPLTPHPHPHPHPTV
Sbjct: 241 AISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASSGPPLTPHPHPHPHPTV 297

BLAST of MC08g1931 vs. ExPASy TrEMBL
Match: A0A6J1G4K7 (uncharacterized protein LOC111450687 OS=Cucurbita moschata OX=3662 GN=LOC111450687 PE=4 SV=1)

HSP 1 Score: 548 bits (1411), Expect = 1.61e-195
Identity = 273/295 (92.54%), Postives = 284/295 (96.27%), Query Frame = 0

Query: 1   MSDHLVLYVDHLIRPVAPVEPVSEPADPVPSAPREAVVDSAAAGPSCSTATAVADDRTVK 60
           MSDHLVLYVDHLI PVAPV+PVS+P DPVPSAPRE V DSAAA PSCSTATA  DDRTV+
Sbjct: 1   MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSCSTATA--DDRTVE 60

Query: 61  QHAPNEEEPLIQAAECRICQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI 120
           Q APNEE+PLIQAAECRICQEED+LNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI
Sbjct: 61  QDAPNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI 120

Query: 121 CHQSYQPGYTAPPRTEETAIDMEGRWTISGNPLDLRDTRLLAIAEAERHFLEAEYDDYAT 180
           CHQSYQPGYTAPPR+EET IDMEGRWTI+GNPLDLRDTRLLAIAEAER+FLEAEYDDYA 
Sbjct: 121 CHQSYQPGYTAPPRSEETVIDMEGRWTIAGNPLDLRDTRLLAIAEAERNFLEAEYDDYAA 180

Query: 181 SNDSGAAFCRSAALIV-ALLLLRHALTITDPDGEDDASAFFSIFLLRAAGFLLPCYIMAW 240
           SNDSGAAFCRSAALI+ ALLLLRHALTITDPDG+DDASAFFSIFLLRAAGFLLPCYIMAW
Sbjct: 181 SNDSGAAFCRSAALILLALLLLRHALTITDPDGDDDASAFFSIFLLRAAGFLLPCYIMAW 240

Query: 241 AISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASSGPPLTPHPHPHPHP 294
           AISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIAS+GPPLTPHPHPHPHP
Sbjct: 241 AISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHP 293

BLAST of MC08g1931 vs. ExPASy TrEMBL
Match: A0A6J1IBB6 (uncharacterized protein LOC111471364 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111471364 PE=4 SV=1)

HSP 1 Score: 533 bits (1372), Expect = 4.87e-190
Identity = 269/296 (90.88%), Postives = 282/296 (95.27%), Query Frame = 0

Query: 1   MSDHLVLYVDHLIRPVAPVEPVSEPADPVPSAPREAVVDSAAAGPSCSTATAVADDRTVK 60
           MSDHLVLYVDHLI PVAPV+PVS+P DPVPSAPRE V DSAAA PS STATA  DDRTV+
Sbjct: 1   MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSSSTATA--DDRTVE 60

Query: 61  QHAPNEEEPLIQAAECRICQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI 120
           Q APNEE+PLIQAAECRICQEED+LNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI
Sbjct: 61  QDAPNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI 120

Query: 121 CHQSYQPGYTAPPRTEETAIDMEGRWTISGNPLDLRDTRLLAIAEAERHFLEAEYDDYAT 180
           CHQSYQPGY+AP R+EET IDMEGRWTI+GNPLDLRDTRLLAIAEAER+FLEAEYDDYA 
Sbjct: 121 CHQSYQPGYSAPLRSEETVIDMEGRWTIAGNPLDLRDTRLLAIAEAERNFLEAEYDDYAA 180

Query: 181 SNDSGAAFCRSAALIV-ALLLLRHALTITDPDGEDD-ASAFFSIFLLRAAGFLLPCYIMA 240
           SNDSGAAFCRSAALI+ ALLLLRHALTITDPDG+DD A+AFFSIFLLRAAGFLLPCYIMA
Sbjct: 181 SNDSGAAFCRSAALILLALLLLRHALTITDPDGDDDDAAAFFSIFLLRAAGFLLPCYIMA 240

Query: 241 WAISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASSGPPLTPHPHPHPHP 294
           WAISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIAS+GPPLTPHPHPHPHP
Sbjct: 241 WAISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHP 294

BLAST of MC08g1931 vs. ExPASy TrEMBL
Match: A0A6J1I5Y1 (uncharacterized protein LOC111471364 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471364 PE=4 SV=1)

HSP 1 Score: 533 bits (1372), Expect = 5.24e-190
Identity = 269/296 (90.88%), Postives = 282/296 (95.27%), Query Frame = 0

Query: 1   MSDHLVLYVDHLIRPVAPVEPVSEPADPVPSAPREAVVDSAAAGPSCSTATAVADDRTVK 60
           MSDHLVLYVDHLI PVAPV+PVS+P DPVPSAPRE V DSAAA PS STATA  DDRTV+
Sbjct: 1   MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSSSTATA--DDRTVE 60

Query: 61  QHAPNEEEPLIQAAECRICQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI 120
           Q APNEE+PLIQAAECRICQEED+LNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI
Sbjct: 61  QDAPNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI 120

Query: 121 CHQSYQPGYTAPPRTEETAIDMEGRWTISGNPLDLRDTRLLAIAEAERHFLEAEYDDYAT 180
           CHQSYQPGY+AP R+EET IDMEGRWTI+GNPLDLRDTRLLAIAEAER+FLEAEYDDYA 
Sbjct: 121 CHQSYQPGYSAPLRSEETVIDMEGRWTIAGNPLDLRDTRLLAIAEAERNFLEAEYDDYAA 180

Query: 181 SNDSGAAFCRSAALIV-ALLLLRHALTITDPDGEDD-ASAFFSIFLLRAAGFLLPCYIMA 240
           SNDSGAAFCRSAALI+ ALLLLRHALTITDPDG+DD A+AFFSIFLLRAAGFLLPCYIMA
Sbjct: 181 SNDSGAAFCRSAALILLALLLLRHALTITDPDGDDDDAAAFFSIFLLRAAGFLLPCYIMA 240

Query: 241 WAISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASSGPPLTPHPHPHPHP 294
           WAISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIAS+GPPLTPHPHPHPHP
Sbjct: 241 WAISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHP 294

BLAST of MC08g1931 vs. ExPASy TrEMBL
Match: A0A6J1KEW0 (uncharacterized protein LOC111492629 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492629 PE=4 SV=1)

HSP 1 Score: 530 bits (1364), Expect = 6.97e-189
Identity = 268/296 (90.54%), Postives = 277/296 (93.58%), Query Frame = 0

Query: 1   MSDHLVLYVDHLIRPVAPVEPVSEPADPVPSAPREAVVDSAAAGPSCSTATAVADDRTVK 60
           M DHLVLYVDHLIRPVAPV+ V+EP DPVPSAPRE VVDSAAAGPSCSTATA  D  TV+
Sbjct: 1   MGDHLVLYVDHLIRPVAPVQSVAEPVDPVPSAPREGVVDSAAAGPSCSTATA--DHPTVE 60

Query: 61  QHAPNEEEPLIQAAECRICQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI 120
           Q APNEEEPLIQ AECRICQEED +NNLETPCACSGSLKYAHRKCVQHWCNEKGDI CEI
Sbjct: 61  QDAPNEEEPLIQVAECRICQEEDGINNLETPCACSGSLKYAHRKCVQHWCNEKGDIICEI 120

Query: 121 CHQSYQPGYTAPPRTEETAIDMEGRWTISGNPLDLRDTRLLAIAEAERHFLEAEYDDYAT 180
           CHQ YQPGYTAP R+EETAIDMEGRWTI+GNPLDLRDTRLLAIAEAERHFLEAEYDDYA 
Sbjct: 121 CHQFYQPGYTAPSRSEETAIDMEGRWTIAGNPLDLRDTRLLAIAEAERHFLEAEYDDYAA 180

Query: 181 SNDSGAAFCRSAALIV-ALLLLRHALTITDPDGEDDASAFFSIFLLRAAGFLLPCYIMAW 240
           SNDSGAAFCRSAALI+ ALLLLRHALTITDPDGEDDASAFFSIFLLRAAGFLLPCYIMAW
Sbjct: 181 SNDSGAAFCRSAALILLALLLLRHALTITDPDGEDDASAFFSIFLLRAAGFLLPCYIMAW 240

Query: 241 AISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASS-GPPLTPHPHPHPHP 294
           AISILQRRRQRQEAAA+AATQVAFVLQHGQHRGLQFAIAS+ G PLTPHPHP PHP
Sbjct: 241 AISILQRRRQRQEAAAVAATQVAFVLQHGQHRGLQFAIASAAGAPLTPHPHPLPHP 294

BLAST of MC08g1931 vs. TAIR 10
Match: AT3G47550.3 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 309.7 bits (792), Expect = 2.6e-84
Identity = 176/297 (59.26%), Postives = 209/297 (70.37%), Query Frame = 0

Query: 1   MSDHLVLYVDHLIRPVAPVEPVSEPADPVPSAPREAVVDSAAAGPSCSTATAVADDRTVK 60
           M+DHL L  D LI         SE  D    +           G   ++++    +   +
Sbjct: 1   MADHLSLCTDRLI--------TSESLDSEKDSDSSGESSYRPQGTDLASSSVNETEVPRE 60

Query: 61  QHA-PNEEEPLIQAAECRICQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCE 120
            +A  +EEEPL+Q+ ECRICQEEDS  NLE PCAC+GSLKYAHRKCVQ WCNEKGDITCE
Sbjct: 61  YYAVADEEEPLLQSVECRICQEEDSTKNLEAPCACNGSLKYAHRKCVQRWCNEKGDITCE 120

Query: 121 ICHQSYQPGYTA--PPRTEETAIDMEGRWTISGNPLDLRDTRLLAIAEAERHFLEAEYDD 180
           ICHQ YQ GYTA  PP  +ET I +   W  +G PLDL D R+LA+A AERHFLEA+YD+
Sbjct: 121 ICHQPYQHGYTAPPPPPPDETIIHIGDDWE-NGVPLDLTDPRILAMAAAERHFLEADYDE 180

Query: 181 YATSNDSGAAFCRSAALI-VALLLLRHALTI-TDPDGEDDASAFFSIFLLRAAGFLLPCY 240
           Y+ +N SGAAFCRSAALI +ALLLLR AL + T+PD EDD +AFFS+FLLRAAGFLLPCY
Sbjct: 181 YSENNSSGAAFCRSAALILMALLLLRDALNLTTNPDDEDDPTAFFSLFLLRAAGFLLPCY 240

Query: 241 IMAWAISILQRRRQRQEAAALAATQVAFVLQHG--QHRGLQFAIASSGPPLTPHPHP 291
           IMAWAI ILQRRRQRQEAAALAA +V F++  G  Q RGL FA+A   PP++  P P
Sbjct: 241 IMAWAIGILQRRRQRQEAAALAAAEVTFMIHGGGPQRRGLHFAVAPQ-PPISNVPTP 287

BLAST of MC08g1931 vs. TAIR 10
Match: AT3G47550.6 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 309.7 bits (792), Expect = 2.6e-84
Identity = 176/297 (59.26%), Postives = 209/297 (70.37%), Query Frame = 0

Query: 1   MSDHLVLYVDHLIRPVAPVEPVSEPADPVPSAPREAVVDSAAAGPSCSTATAVADDRTVK 60
           M+DHL L  D LI         SE  D    +           G   ++++    +   +
Sbjct: 1   MADHLSLCTDRLI--------TSESLDSEKDSDSSGESSYRPQGTDLASSSVNETEVPRE 60

Query: 61  QHA-PNEEEPLIQAAECRICQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCE 120
            +A  +EEEPL+Q+ ECRICQEEDS  NLE PCAC+GSLKYAHRKCVQ WCNEKGDITCE
Sbjct: 61  YYAVADEEEPLLQSVECRICQEEDSTKNLEAPCACNGSLKYAHRKCVQRWCNEKGDITCE 120

Query: 121 ICHQSYQPGYTA--PPRTEETAIDMEGRWTISGNPLDLRDTRLLAIAEAERHFLEAEYDD 180
           ICHQ YQ GYTA  PP  +ET I +   W  +G PLDL D R+LA+A AERHFLEA+YD+
Sbjct: 121 ICHQPYQHGYTAPPPPPPDETIIHIGDDWE-NGVPLDLTDPRILAMAAAERHFLEADYDE 180

Query: 181 YATSNDSGAAFCRSAALI-VALLLLRHALTI-TDPDGEDDASAFFSIFLLRAAGFLLPCY 240
           Y+ +N SGAAFCRSAALI +ALLLLR AL + T+PD EDD +AFFS+FLLRAAGFLLPCY
Sbjct: 181 YSENNSSGAAFCRSAALILMALLLLRDALNLTTNPDDEDDPTAFFSLFLLRAAGFLLPCY 240

Query: 241 IMAWAISILQRRRQRQEAAALAATQVAFVLQHG--QHRGLQFAIASSGPPLTPHPHP 291
           IMAWAI ILQRRRQRQEAAALAA +V F++  G  Q RGL FA+A   PP++  P P
Sbjct: 241 IMAWAIGILQRRRQRQEAAALAAAEVTFMIHGGGPQRRGLHFAVAPQ-PPISNVPTP 287

BLAST of MC08g1931 vs. TAIR 10
Match: AT5G62460.1 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 305.1 bits (780), Expect = 6.3e-83
Identity = 174/294 (59.18%), Postives = 211/294 (71.77%), Query Frame = 0

Query: 19  VEPVSEPADPVPSAPREAVVDSAAAGPSCSTATAVA--------DDRTVKQHAPNEEEPL 78
           V+P S  +D   +   E+  +S+ A  S S +T  +        ++ T ++   +EEEPL
Sbjct: 13  VKPESLNSDRGSNQSIESSGESSRAQGSTSLSTTKSMDGKKTEEEETTEQRDVDDEEEPL 72

Query: 79  IQAAECRICQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICHQSYQPGYT 138
           IQ+ ECRICQEEDS+ NLE+PC+CSGSLKYAHRKCVQ WCNEKGD TCEICH+SYQPGYT
Sbjct: 73  IQSVECRICQEEDSVKNLESPCSCSGSLKYAHRKCVQRWCNEKGDTTCEICHKSYQPGYT 132

Query: 139 A--PPRTEETAIDMEGRWTISGNPLDLRDTRLLAIAEAERHFLEAEYDDYATSNDSGAAF 198
           A  PP  ++T ID+   W  +G  LDL D R+LA+A AERHF +A+YD+YA SN SGAAF
Sbjct: 133 APPPPPADDTIIDIGEDWG-NGVHLDLNDPRILAMAAAERHFFDADYDEYADSNSSGAAF 192

Query: 199 CRSAALI-VALLLLRHALTITD---PDGEDDASAFFSIFLLRAAGFLLPCYIMAWAISIL 258
           CRSAALI +ALLLLRHAL +T+    D EDD SAFF +F+LRAAGFLLPCYIMAWAISIL
Sbjct: 193 CRSAALILMALLLLRHALNLTNNNSDDEEDDPSAFFFLFMLRAAGFLLPCYIMAWAISIL 252

Query: 259 QRRRQRQEAAALAATQVAFVLQHG------QHRGLQFAIASSGPPLTPHPHPHP 293
           QRRRQRQEAAALAA +VAF+L  G      +  GL FA+    P L  +PH  P
Sbjct: 253 QRRRQRQEAAALAAAEVAFMLHSGGGGGGQRRGGLHFAVP---PELISNPHHQP 302

BLAST of MC08g1931 vs. TAIR 10
Match: AT2G02960.2 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 299.7 bits (766), Expect = 2.6e-81
Identity = 159/220 (72.27%), Postives = 178/220 (80.91%), Query Frame = 0

Query: 65  NEEEPLIQAAECRICQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICHQS 124
           +E EPLI + ECRIC +E  + NLE+PCACSGSLKYAHRKCVQ WCNEKG+I CEICHQ 
Sbjct: 32  DENEPLIVSGECRICSDESPVENLESPCACSGSLKYAHRKCVQRWCNEKGNIICEICHQP 91

Query: 125 YQPGYTAPP---RTEETAIDMEGRWTISGNPLDLRDTRLLAIAEAERHFLEAEYDDYATS 184
           YQPGYTAPP   + EET ID+ G WTISG  LD+ D RLLAIAEAER +LE+EY +Y  S
Sbjct: 92  YQPGYTAPPPPLQPEETTIDIGGGWTISG--LDVHDPRLLAIAEAERRYLESEYVEYTAS 151

Query: 185 NDSGAAFCRSAALI-VALLLLRHALTIT-DPDG-EDDASAFFSIFLLRAAGFLLPCYIMA 244
           + SGAAFCRSAALI +ALLLLRHALTIT D DG EDD S+  S+ LLRAAGFLLPCYIMA
Sbjct: 152 SASGAAFCRSAALILMALLLLRHALTITDDTDGEEDDPSSILSLVLLRAAGFLLPCYIMA 211

Query: 245 WAISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIA 279
           WAISILQRRRQRQEAAAL ATQ A VLQ GQ R + F ++
Sbjct: 212 WAISILQRRRQRQEAAAL-ATQFALVLQSGQPRTVHFTVS 248

BLAST of MC08g1931 vs. TAIR 10
Match: AT2G02960.4 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 299.7 bits (766), Expect = 2.6e-81
Identity = 159/220 (72.27%), Postives = 178/220 (80.91%), Query Frame = 0

Query: 65  NEEEPLIQAAECRICQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICHQS 124
           +E EPLI + ECRIC +E  + NLE+PCACSGSLKYAHRKCVQ WCNEKG+I CEICHQ 
Sbjct: 32  DENEPLIVSGECRICSDESPVENLESPCACSGSLKYAHRKCVQRWCNEKGNIICEICHQP 91

Query: 125 YQPGYTAPP---RTEETAIDMEGRWTISGNPLDLRDTRLLAIAEAERHFLEAEYDDYATS 184
           YQPGYTAPP   + EET ID+ G WTISG  LD+ D RLLAIAEAER +LE+EY +Y  S
Sbjct: 92  YQPGYTAPPPPLQPEETTIDIGGGWTISG--LDVHDPRLLAIAEAERRYLESEYVEYTAS 151

Query: 185 NDSGAAFCRSAALI-VALLLLRHALTIT-DPDG-EDDASAFFSIFLLRAAGFLLPCYIMA 244
           + SGAAFCRSAALI +ALLLLRHALTIT D DG EDD S+  S+ LLRAAGFLLPCYIMA
Sbjct: 152 SASGAAFCRSAALILMALLLLRHALTITDDTDGEEDDPSSILSLVLLRAAGFLLPCYIMA 211

Query: 245 WAISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIA 279
           WAISILQRRRQRQEAAAL ATQ A VLQ GQ R + F ++
Sbjct: 212 WAISILQRRRQRQEAAAL-ATQFALVLQSGQPRTVHFTVS 248

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0JN694.2e-0732.69E3 ubiquitin-protein ligase MARCHF3 OS=Bos taurus OX=9913 GN=MARCHF3 PE=2 SV=1[more]
Q86UD34.2e-0732.69E3 ubiquitin-protein ligase MARCHF3 OS=Homo sapiens OX=9606 GN=MARCHF3 PE=1 SV=1[more]
Q8BRX94.2e-0732.69E3 ubiquitin-protein ligase MARCHF3 OS=Mus musculus OX=10090 GN=Marchf3 PE=2 SV=... [more]
Q5XIE54.2e-0732.69E3 ubiquitin-protein ligase MARCHF3 OS=Rattus norvegicus OX=10116 GN=Marchf3 PE=... [more]
Q99M026.1e-0628.00E3 ubiquitin-protein ligase MARCHF2 OS=Mus musculus OX=10090 GN=MARCHF2 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
XP_022144437.12.62e-21399.33uncharacterized protein LOC111014120 [Momordica charantia][more]
XP_038890663.12.65e-19792.31uncharacterized protein LOC120080164 [Benincasa hispida] >XP_038890664.1 unchara... [more]
XP_022946694.13.33e-19592.54uncharacterized protein LOC111450687 [Cucurbita moschata][more]
XP_023547303.17.88e-19391.53uncharacterized protein LOC111806165 [Cucurbita pepo subsp. pepo][more]
XP_022972853.11.01e-18990.88uncharacterized protein LOC111471364 isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1CTP21.27e-21399.33uncharacterized protein LOC111014120 OS=Momordica charantia OX=3673 GN=LOC111014... [more]
A0A6J1G4K71.61e-19592.54uncharacterized protein LOC111450687 OS=Cucurbita moschata OX=3662 GN=LOC1114506... [more]
A0A6J1IBB64.87e-19090.88uncharacterized protein LOC111471364 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1I5Y15.24e-19090.88uncharacterized protein LOC111471364 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1KEW06.97e-18990.54uncharacterized protein LOC111492629 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
AT3G47550.32.6e-8459.26RING/FYVE/PHD zinc finger superfamily protein [more]
AT3G47550.62.6e-8459.26RING/FYVE/PHD zinc finger superfamily protein [more]
AT5G62460.16.3e-8359.18RING/FYVE/PHD zinc finger superfamily protein [more]
AT2G02960.22.6e-8172.27RING/FYVE/PHD zinc finger superfamily protein [more]
AT2G02960.42.6e-8172.27RING/FYVE/PHD zinc finger superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011016Zinc finger, RING-CH-typeSMARTSM00744ringv_2coord: 75..122
e-value: 5.2E-21
score: 85.8
IPR011016Zinc finger, RING-CH-typePFAMPF12906RINGvcoord: 76..121
e-value: 5.8E-13
score: 48.7
IPR011016Zinc finger, RING-CH-typePROSITEPS51292ZF_RING_CHcoord: 68..128
score: 22.806822
IPR022143Protein of unknown function DUF3675PFAMPF12428DUF3675coord: 127..241
e-value: 4.7E-40
score: 136.8
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 61..131
e-value: 1.4E-19
score: 71.4
NoneNo IPR availablePANTHERPTHR23012:SF181E3 UBIQUITIN-PROTEIN LIGASE MARCH-RELATEDcoord: 1..276
NoneNo IPR availableCDDcd16495RING_CH-C4HC3_MARCHcoord: 76..122
e-value: 6.25095E-19
score: 76.5972
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 74..126
IPR033275E3 ubiquitin-protein ligase MARCH-likePANTHERPTHR23012RING/FYVE/PHD ZINC FINGER DOMAIN-CONTAININGcoord: 1..276

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g1931.1MC08g1931.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004842 ubiquitin-protein transferase activity
molecular_function GO:0008270 zinc ion binding