MC08g1401 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g1401
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionribonuclease MC-like
LocationMC08: 14936534 .. 14937630 (-)
RNA-Seq ExpressionMC08g1401
SyntenyMC08g1401
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGCAGAGAGAAATTATTGTGTTGGTTTTAAGTATGTTGGTATTGGTAGCAATTTGTGAGGGACTCAACGAGTTTGACTACTTGCAGGTTGTGATTCAATGGCAACCAGCTACTTGCAGTGATTATAATAAGCCTGCATGCTTTCAAGCCCCAGGTAATAGATTCAGTATCCATGGCGTGTGGCCCAGCCTATACGATGGCTATCCAACCCAATGTTCCGGTAGTCCCTTCGATAGGAATCAGGTATATTTATGTGTTCCCATATCTAGCTTGTTTCTCGTTTTCAGTTTTATTAAGAAATTTAAATCTATGACAAACTATTTATGTTTCTTGTTACTAATTTTTTATGAACAATCTCAAAATTGTAAACAAATTTTAGAAACAACAAAAACAAATTTCTTCTTTTGTCATTCGAATTCTTGGATCTGTGTCTGTTAGGAGTCGTGCCATTCTATTTTTCTTTATTAAAAAAAAGGAAATAAAGACAAGAGACGAGAATGTTATAAACGGGCTCTACATATTCTTTAAATTTGGTTCATTTAGTCGTTGATATCGTGTAACTATTGTATATGAAAATAGTGAGAACCAGTTGAAAAAAAACACCCTAACATTAAACAGTAAACCCTATTTTAAAAAAAATTTATTGAGTCTCCAGATCAGCGGACTACAACCCGGAATCAGCGAAATTTGGCCAAACGTAGCGACAGGCGACAACTTTTTGGCTGTGGCAACACGAGTGGGACAAGCATGGAACATGCAGCGAATCAACATTTTATGACCAGCAAGAATATTTCGGATTTGGAATGGATGCATATATTCATAACAATCTCCAAGTTATTCTGAGTAACAACCAAATTACACCCAACGGACGAACATACACAAGGGATCAAGTGTATGCAGCAATTAGAGCAAAAACTGGAAAGACGCCAGCCGTTCGATGCAACTACAACAGCAGGACTGGAGCATCGCAATTGCATGAAATATCTCTGTGTTTCAAGAAGGATGCTTCTGTTTTTGAGGACTGCCCCTTAAACGCCAATAAATGTGGTGCCTCTTTCGTTTGGAATTCGTGGAACACTGTGAAGGCTATATCT

mRNA sequence

ATGGCGCAGAGAGAAATTATTGTGTTGGTTTTAAGTATGTTGGTATTGGTAGCAATTTGTGAGGGACTCAACGAGTTTGACTACTTGCAGGTTGTGATTCAATGGCAACCAGCTACTTGCAGTGATTATAATAAGCCTGCATGCTTTCAAGCCCCAGGTAATAGATTCAGTATCCATGGCGTGTGGCCCAGCCTATACGATGGCTATCCAACCCAATGTTCCGGTAGTCCCTTCGATAGGAATCAGATCAGCGGACTACAACCCGGAATCAGCGAAATTTGGCCAAACGTAGCGACAGGCGACAACTGGCTGTGGCAACACGAGTGGGACAAGCATGGAACATGCAGCGAATCAACATTTTATGACCAGCAAGAATATTTCGGATTTGGAATGGATGCATATATTCATAACAATCTCCAAGTTATTCTGAGTAACAACCAAATTACACCCAACGGACGAACATACACAAGGGATCAAGTGTATGCAGCAATTAGAGCAAAAACTGGAAAGACGCCAGCCGTTCGATGCAACTACAACAGCAGGACTGGAGCATCGCAATTGCATGAAATATCTCTGTGTTTCAAGAAGGATGCTTCTGTTTTTGAGGACTGCCCCTTAAACGCCAATAAATGTGGTGCCTCTTTCGTTTGGAATTCGTGGAACACTGTGAAGGCTATATCT

Coding sequence (CDS)

ATGGCGCAGAGAGAAATTATTGTGTTGGTTTTAAGTATGTTGGTATTGGTAGCAATTTGTGAGGGACTCAACGAGTTTGACTACTTGCAGGTTGTGATTCAATGGCAACCAGCTACTTGCAGTGATTATAATAAGCCTGCATGCTTTCAAGCCCCAGGTAATAGATTCAGTATCCATGGCGTGTGGCCCAGCCTATACGATGGCTATCCAACCCAATGTTCCGGTAGTCCCTTCGATAGGAATCAGATCAGCGGACTACAACCCGGAATCAGCGAAATTTGGCCAAACGTAGCGACAGGCGACAACTGGCTGTGGCAACACGAGTGGGACAAGCATGGAACATGCAGCGAATCAACATTTTATGACCAGCAAGAATATTTCGGATTTGGAATGGATGCATATATTCATAACAATCTCCAAGTTATTCTGAGTAACAACCAAATTACACCCAACGGACGAACATACACAAGGGATCAAGTGTATGCAGCAATTAGAGCAAAAACTGGAAAGACGCCAGCCGTTCGATGCAACTACAACAGCAGGACTGGAGCATCGCAATTGCATGAAATATCTCTGTGTTTCAAGAAGGATGCTTCTGTTTTTGAGGACTGCCCCTTAAACGCCAATAAATGTGGTGCCTCTTTCGTTTGGAATTCGTGGAACACTGTGAAGGCTATATCT

Protein sequence

MAQREIIVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYNKPACFQAPGNRFSIHGVWPSLYDGYPTQCSGSPFDRNQISGLQPGISEIWPNVATGDNWLWQHEWDKHGTCSESTFYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRCNYNSRTGASQLHEISLCFKKDASVFEDCPLNANKCGASFVWNSWNTVKAIS
Homology
BLAST of MC08g1401 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 5.3e-34
Identity = 80/196 (40.82%), Postives = 108/196 (55.10%), Query Frame = 0

Query: 26  FDYLQVVIQWQPATCSDYNKPACFQAPGNRFSIHGVWPSLYDGYPTQC-SGSPFDRNQIS 85
           FD    V QW PA CS     +C  +    F+IHG+WP       T C  GSPFD  +IS
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKIS 60

Query: 86  GLQPGISEIWPNVATGDN-WLWQHEWDKHGTCSESTFYDQQEYFGFGMDAYIHNNLQVI- 145
            LQ  ++ +WPNV   +N   W HEW KHGTCSESTF +Q  YF   +D  + NN  +I 
Sbjct: 61  HLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTF-NQAAYFKLAVD--MRNNYDIIG 120

Query: 146 -LSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRCNYNSRTGASQLHEISLCFKKDASVF 205
            L  +   PNGRT +R  +   ++AK GK P +RC  + +T  S L ++  CF +D S  
Sbjct: 121 ALRPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGSTL 180

Query: 206 EDCPLNANKCGASFVW 218
            DC    + CGA+F++
Sbjct: 181 IDC--TRDTCGANFIF 191

BLAST of MC08g1401 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 133.3 bits (334), Expect = 3.5e-30
Identity = 78/226 (34.51%), Postives = 117/226 (51.77%), Query Frame = 0

Query: 1   MAQREIIVLVLSMLVLVAICEGLN---EFDYLQVVIQWQPATCSDYNKPACFQA---PGN 60
           MA      L L +L++      LN   +FD+   V QW P +  D  +  C+     P  
Sbjct: 1   MASNSAFSLFLILLIITQCLSVLNAAKDFDFFYFVQQW-PGSYCDTKQSCCYPTTGKPAA 60

Query: 61  RFSIHGVWPSLYDG-YPTQCS-GSPFDRNQISGLQPGISEIWPNVAT---GDNWLWQHEW 120
            F IHG+WP+  DG YP+ C   SP+D++QIS L   + + WP +A      +  W HEW
Sbjct: 61  DFGIHGLWPNNNDGTYPSNCDPNSPYDQSQISDLISSMQQNWPTLACPSGSGSTFWSHEW 120

Query: 121 DKHGTCSESTFYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTG 180
           +KHGTC+ES   +Q  YF   +D     +L  IL    I P+G +Y    +  AI++  G
Sbjct: 121 EKHGTCAESVLTNQHAYFKKALDLKNQIDLLSILQGADIHPDGESYDLVNIRNAIKSAIG 180

Query: 181 KTPAVRCNYNSRTGASQLHEISLCFKKDASVFEDCPL-NANKCGAS 215
            TP ++CN + ++G SQL+++ +C     S   +CP+    KCG S
Sbjct: 181 YTPWIQCNVD-QSGNSQLYQVYICVDGSGSSLIECPIFPGGKCGTS 224

BLAST of MC08g1401 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 3.5e-30
Identity = 78/224 (34.82%), Postives = 119/224 (53.12%), Query Frame = 0

Query: 6   IIVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYNKPACFQ---APGNRFSIHGVW 65
           +I L++ +  + +      +FD+   V QW P +  D  K  C+     P   F IHG+W
Sbjct: 10  LISLLVILPSVFSASSSSEDFDFFYFVQQW-PGSYCDTQKKCCYPNSGKPAADFGIHGLW 69

Query: 66  PSLYDG-YPTQCSGS-PFDRNQISGLQPGISEIWPNVAT---GDNWLWQHEWDKHGTCSE 125
           P+  DG YP+ C  S PFD + IS L   + + WP +A         W+HEW+KHGTCSE
Sbjct: 70  PNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSE 129

Query: 126 STFYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRCN 185
           S   DQ EYF   ++     NL   L+   I P+G++Y+ + +  +I+   G TP V CN
Sbjct: 130 SVI-DQHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECN 189

Query: 186 YNSRTGASQLHEISLCFKKDASVFEDCPLNAN-KCGASFVWNSW 221
            +  +G SQL+++ LC  +  S   +CP+  + KCGA   + S+
Sbjct: 190 RDG-SGNSQLYQVYLCVDRSGSGLIECPVFPHGKCGAEIEFPSF 230

BLAST of MC08g1401 vs. ExPASy Swiss-Prot
Match: Q40966 (Ribonuclease S-4 OS=Pyrus pyrifolia OX=3767 PE=1 SV=2)

HSP 1 Score: 114.8 bits (286), Expect = 1.3e-24
Identity = 69/206 (33.50%), Postives = 104/206 (50.49%), Query Frame = 0

Query: 6   IIVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYNKPACFQAPGNRFSIHGVWPSL 65
           +  +VLS++VL+     +  FDY Q   Q+QPA C+  N   C       F++HG+WPS 
Sbjct: 9   MFTMVLSLIVLIFSASTVG-FDYFQFTQQYQPAVCNS-NPTPCNDPTDKLFTVHGLWPSN 68

Query: 66  YDG-YPTQCSGSPFDRNQISGLQPGISEIWPNVATGDNWL--WQHEWDKHGTCSESTFYD 125
            +G  P +C  +  +  +I  +   +  IWPNV    + +  W+ EW KHGTC   T  D
Sbjct: 69  RNGPDPEKCKTTTMNSQKIGNMTAQLEIIWPNVLNRSDHVGFWEREWLKHGTCGYPTIKD 128

Query: 126 QQEYFGFGMDAYI--HNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKT-PAVRCNYN 185
              Y    +  YI    N+  ILS   I PNG   +   +  AIR+    T P  +C  N
Sbjct: 129 DMHYLKTVIKMYITQKQNVSAILSKATIQPNGNNRSLVDIENAIRSGNNNTKPKFKCQKN 188

Query: 186 SRTGASQLHEISLCFKKDASVFEDCP 206
           +RT  ++L E++LC  +D + F +CP
Sbjct: 189 TRT-TTELVEVTLCSNRDLTKFINCP 211

BLAST of MC08g1401 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 2.2e-24
Identity = 71/209 (33.97%), Postives = 104/209 (49.76%), Query Frame = 0

Query: 6   IIVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYNKPACFQ---APGNRFSIHGVW 65
           I +L L  L + +  +   +FD+   V+QW  A C D     C+     P   F IHG+W
Sbjct: 5   IFILALQQLYVQSFAQ---DFDFFYFVLQWPGAYC-DSRHSCCYPQTGKPAADFGIHGLW 64

Query: 66  PSL-YDGYPTQCS-GSPFDRNQISGLQPGISEIWPNVATGDN---WLWQHEWDKHGTCSE 125
           P+    G+P  C+  S FD  ++S L   +   WP ++   N     W HEW+KHGTC+E
Sbjct: 65  PNYKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAE 124

Query: 126 STFYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRCN 185
           S   DQ +YF  G+      NL   L+N  I P+ + Y    +   I+   G  P + CN
Sbjct: 125 SEL-DQHDYFEAGLKLKQKANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECN 184

Query: 186 YNSRTGASQLHEISLCFKKDASVFEDCPL 207
            +S +  SQL++I LC    AS F +CP+
Sbjct: 185 KDS-SHNSQLYQIYLCVDTSASKFINCPV 207

BLAST of MC08g1401 vs. NCBI nr
Match: XP_022149793.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 482 bits (1241), Expect = 3.80e-172
Identity = 227/228 (99.56%), Postives = 227/228 (99.56%), Query Frame = 0

Query: 1   MAQREIIVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYNKPACFQAPGNRFSIHG 60
           MAQREIIVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYNKPACFQAPGNRFSIHG
Sbjct: 1   MAQREIIVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYNKPACFQAPGNRFSIHG 60

Query: 61  VWPSLYDGYPTQCSGSPFDRNQISGLQPGISEIWPNVATGDN-WLWQHEWDKHGTCSEST 120
           VWPSLYDGYPTQCSGSPFDRNQISGLQPGISEIWPNVATGDN WLWQHEWDKHGTCSEST
Sbjct: 61  VWPSLYDGYPTQCSGSPFDRNQISGLQPGISEIWPNVATGDNFWLWQHEWDKHGTCSEST 120

Query: 121 FYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRCNYN 180
           FYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRCNYN
Sbjct: 121 FYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRCNYN 180

Query: 181 SRTGASQLHEISLCFKKDASVFEDCPLNANKCGASFVWNSWNTVKAIS 227
           SRTGASQLHEISLCFKKDASVFEDCPLNANKCGASFVWNSWNTVKAIS
Sbjct: 181 SRTGASQLHEISLCFKKDASVFEDCPLNANKCGASFVWNSWNTVKAIS 228

BLAST of MC08g1401 vs. NCBI nr
Match: AEM42996.1 (S-RNase [Siraitia grosvenorii])

HSP 1 Score: 347 bits (891), Expect = 8.69e-119
Identity = 165/230 (71.74%), Postives = 184/230 (80.00%), Query Frame = 0

Query: 1   MAQREIIVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYNKP--ACFQAPGNRFSI 60
           MA+REI VLVLSMLVL+A CEGLNEFDYLQVV+QWQPATCS  NKP   C+Q P NR+SI
Sbjct: 1   MAKREI-VLVLSMLVLIASCEGLNEFDYLQVVMQWQPATCSASNKPYPICYQNPDNRYSI 60

Query: 61  HGVWPSLYDGYPTQCSGSPFDRNQISGLQPGISEIWPNVATGDN-WLWQHEWDKHGTCSE 120
           HGVWPSLY G  TQCSGSPF+ N IS LQPGISEIWPN+  G+N WLW HEWDKHGTC+E
Sbjct: 61  HGVWPSLYSGAATQCSGSPFNANAISILQPGISEIWPNIINGNNLWLWGHEWDKHGTCTE 120

Query: 121 STFYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRCN 180
              +DQ  YF F MD YI  NL V+L N+QITPNG+ Y+RDQVYAAIRAKTGKTPAVRCN
Sbjct: 121 EILFDQDRYFSFAMDTYIDYNLLVLLGNSQITPNGQIYSRDQVYAAIRAKTGKTPAVRCN 180

Query: 181 YNSRTGASQLHEISLCFKKDASVFEDCPLNANKCGASFVWNSWNTVKAIS 227
           YN  TG  Q+HE+SLC+  DAS   DCPLNANKC   FVW SW+T KA+S
Sbjct: 181 YNRWTGEQQMHEVSLCYNHDASAVVDCPLNANKCDNYFVWYSWSTQKAVS 229

BLAST of MC08g1401 vs. NCBI nr
Match: XP_038903744.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 333 bits (855), Expect = 2.63e-113
Identity = 155/229 (67.69%), Postives = 183/229 (79.91%), Query Frame = 0

Query: 3   QREIIVLVLSMLVL-VAICEGLNEFDYLQVVIQWQPATCSDYNKPA--CFQAPGNRFSIH 62
           +++ I L+LSMLV+ V+ CEG+N++DYLQVVIQWQPATC+D+ KP   CFQ P NRFSIH
Sbjct: 2   EKKEIGLILSMLVMIVSCCEGVNDYDYLQVVIQWQPATCTDWKKPYPYCFQDPDNRFSIH 61

Query: 63  GVWPSLYDGYPTQCSGSPFDRNQISGLQPGISEIWPNVATGDN-WLWQHEWDKHGTCSES 122
           G+WPSLY G  T C GSPFD NQIS LQPGISEIWPNV TG N WLW HEWDKHGTC+ES
Sbjct: 62  GMWPSLYSGAQTHCFGSPFDVNQISILQPGISEIWPNVVTGKNQWLWGHEWDKHGTCTES 121

Query: 123 TFYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRCNY 182
             +DQ+EYF F MD YI NNL  IL  +QITPNG+ Y+R Q+YAAI AKTGKTPAVRCNY
Sbjct: 122 ILFDQEEYFSFTMDKYIENNLSFILGKSQITPNGQLYSRAQIYAAISAKTGKTPAVRCNY 181

Query: 183 NSRTGASQLHEISLCFKKDASVFEDCPLNANKCGASFVWNSWNTVKAIS 227
           N  +G  QLHEI+LC+  +A+ FEDCPLNANKC  +F+W  W+T KA+S
Sbjct: 182 NYWSGVEQLHEITLCYNWNATAFEDCPLNANKCDNTFMWYPWSTTKALS 230

BLAST of MC08g1401 vs. NCBI nr
Match: XP_022158939.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 234 bits (598), Expect = 2.95e-74
Identity = 118/224 (52.68%), Postives = 146/224 (65.18%), Query Frame = 0

Query: 1   MAQREIIVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYN-KPACFQAPGNRFSIH 60
           M +REI+ LVLSM + ++  EG+NEFDY QVV+QWQPATCS    K  C Q P +R+S+H
Sbjct: 1   MGKREIL-LVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVH 60

Query: 61  GVWPSLYDGYPTQCSGSP-FDRNQISGLQPGISEIWPNVATGDN-WLWQHEWDKHGTCSE 120
           GVWPSL  GY   CSG+P +D   I  +Q  + + WPNV TG N   W +EW KHGTCS 
Sbjct: 61  GVWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCS- 120

Query: 121 STFYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRCN 180
           S + +Q  YF FG+  Y + +LQ IL    I PNG+ YT+  V  AI + TGK PA+RCN
Sbjct: 121 SAYLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCN 180

Query: 181 YNSRTGASQLHEISLCFKKDASVFEDCPLNANKCGASFVWNSWN 221
            N RTG  QLHEISLCF K+  +  DCP N   C   FVWNSW+
Sbjct: 181 NNYRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSWS 222

BLAST of MC08g1401 vs. NCBI nr
Match: XP_022156540.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 212 bits (540), Expect = 1.94e-65
Identity = 114/226 (50.44%), Postives = 142/226 (62.83%), Query Frame = 0

Query: 1   MAQREIIVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYNK-PACFQAPGNRFSIH 60
           MA+R  IVL+LSML+L+   EG+NEFDY QVV+QWQPATCS       C Q P  RFSIH
Sbjct: 1   MAKRSEIVLILSMLLLIGWSEGVNEFDYYQVVLQWQPATCSSSTLVDECVQPPDERFSIH 60

Query: 61  GVWPSLYDGYPTQCSGSP-FDRNQI-SGLQPGISEIWPNVATG-DNWLWQHEWDKHGTCS 120
           GVWPSL  G    CSG+P +D  Q+ S ++  + + WP+V    D   W HEW  HGTCS
Sbjct: 61  GVWPSLLSG-DAVCSGTPPYDGTQLNSSIRARLHQDWPSVVHRVDPPFWSHEWTLHGTCS 120

Query: 121 ESTFYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRC 180
            + + DQ  YF FG+  Y + ++  IL+   + PNG  Y R  V   I + TGK PA+RC
Sbjct: 121 YA-YLDQFHYFDFGLFVYGNYDVLGILNYYNVYPNGNLYARSDVENIIYSATGKWPALRC 180

Query: 181 NYNSRTGASQLHEISLCFKKDASVFEDCPLNANKCGASFVWNSWNT 222
           N N  +G  QLHEISLCF+K     EDCP NA KC   FVWNSW++
Sbjct: 181 NNNFWSGDVQLHEISLCFRKQDFSLEDCPYNAVKCNDYFVWNSWSS 224

BLAST of MC08g1401 vs. ExPASy TrEMBL
Match: A0A6J1D7Q5 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111018138 PE=3 SV=1)

HSP 1 Score: 482 bits (1241), Expect = 1.84e-172
Identity = 227/228 (99.56%), Postives = 227/228 (99.56%), Query Frame = 0

Query: 1   MAQREIIVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYNKPACFQAPGNRFSIHG 60
           MAQREIIVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYNKPACFQAPGNRFSIHG
Sbjct: 1   MAQREIIVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYNKPACFQAPGNRFSIHG 60

Query: 61  VWPSLYDGYPTQCSGSPFDRNQISGLQPGISEIWPNVATGDN-WLWQHEWDKHGTCSEST 120
           VWPSLYDGYPTQCSGSPFDRNQISGLQPGISEIWPNVATGDN WLWQHEWDKHGTCSEST
Sbjct: 61  VWPSLYDGYPTQCSGSPFDRNQISGLQPGISEIWPNVATGDNFWLWQHEWDKHGTCSEST 120

Query: 121 FYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRCNYN 180
           FYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRCNYN
Sbjct: 121 FYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRCNYN 180

Query: 181 SRTGASQLHEISLCFKKDASVFEDCPLNANKCGASFVWNSWNTVKAIS 227
           SRTGASQLHEISLCFKKDASVFEDCPLNANKCGASFVWNSWNTVKAIS
Sbjct: 181 SRTGASQLHEISLCFKKDASVFEDCPLNANKCGASFVWNSWNTVKAIS 228

BLAST of MC08g1401 vs. ExPASy TrEMBL
Match: K7NBR4 (S-RNase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)

HSP 1 Score: 347 bits (891), Expect = 4.21e-119
Identity = 165/230 (71.74%), Postives = 184/230 (80.00%), Query Frame = 0

Query: 1   MAQREIIVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYNKP--ACFQAPGNRFSI 60
           MA+REI VLVLSMLVL+A CEGLNEFDYLQVV+QWQPATCS  NKP   C+Q P NR+SI
Sbjct: 1   MAKREI-VLVLSMLVLIASCEGLNEFDYLQVVMQWQPATCSASNKPYPICYQNPDNRYSI 60

Query: 61  HGVWPSLYDGYPTQCSGSPFDRNQISGLQPGISEIWPNVATGDN-WLWQHEWDKHGTCSE 120
           HGVWPSLY G  TQCSGSPF+ N IS LQPGISEIWPN+  G+N WLW HEWDKHGTC+E
Sbjct: 61  HGVWPSLYSGAATQCSGSPFNANAISILQPGISEIWPNIINGNNLWLWGHEWDKHGTCTE 120

Query: 121 STFYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRCN 180
              +DQ  YF F MD YI  NL V+L N+QITPNG+ Y+RDQVYAAIRAKTGKTPAVRCN
Sbjct: 121 EILFDQDRYFSFAMDTYIDYNLLVLLGNSQITPNGQIYSRDQVYAAIRAKTGKTPAVRCN 180

Query: 181 YNSRTGASQLHEISLCFKKDASVFEDCPLNANKCGASFVWNSWNTVKAIS 227
           YN  TG  Q+HE+SLC+  DAS   DCPLNANKC   FVW SW+T KA+S
Sbjct: 181 YNRWTGEQQMHEVSLCYNHDASAVVDCPLNANKCDNYFVWYSWSTQKAVS 229

BLAST of MC08g1401 vs. ExPASy TrEMBL
Match: A0A6J1E0X1 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1)

HSP 1 Score: 234 bits (598), Expect = 1.43e-74
Identity = 118/224 (52.68%), Postives = 146/224 (65.18%), Query Frame = 0

Query: 1   MAQREIIVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYN-KPACFQAPGNRFSIH 60
           M +REI+ LVLSM + ++  EG+NEFDY QVV+QWQPATCS    K  C Q P +R+S+H
Sbjct: 1   MGKREIL-LVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVH 60

Query: 61  GVWPSLYDGYPTQCSGSP-FDRNQISGLQPGISEIWPNVATGDN-WLWQHEWDKHGTCSE 120
           GVWPSL  GY   CSG+P +D   I  +Q  + + WPNV TG N   W +EW KHGTCS 
Sbjct: 61  GVWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCS- 120

Query: 121 STFYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRCN 180
           S + +Q  YF FG+  Y + +LQ IL    I PNG+ YT+  V  AI + TGK PA+RCN
Sbjct: 121 SAYLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCN 180

Query: 181 YNSRTGASQLHEISLCFKKDASVFEDCPLNANKCGASFVWNSWN 221
            N RTG  QLHEISLCF K+  +  DCP N   C   FVWNSW+
Sbjct: 181 NNYRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSWS 222

BLAST of MC08g1401 vs. ExPASy TrEMBL
Match: A0A6J1DQK2 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111023418 PE=3 SV=1)

HSP 1 Score: 212 bits (540), Expect = 9.38e-66
Identity = 114/226 (50.44%), Postives = 142/226 (62.83%), Query Frame = 0

Query: 1   MAQREIIVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYNK-PACFQAPGNRFSIH 60
           MA+R  IVL+LSML+L+   EG+NEFDY QVV+QWQPATCS       C Q P  RFSIH
Sbjct: 1   MAKRSEIVLILSMLLLIGWSEGVNEFDYYQVVLQWQPATCSSSTLVDECVQPPDERFSIH 60

Query: 61  GVWPSLYDGYPTQCSGSP-FDRNQI-SGLQPGISEIWPNVATG-DNWLWQHEWDKHGTCS 120
           GVWPSL  G    CSG+P +D  Q+ S ++  + + WP+V    D   W HEW  HGTCS
Sbjct: 61  GVWPSLLSG-DAVCSGTPPYDGTQLNSSIRARLHQDWPSVVHRVDPPFWSHEWTLHGTCS 120

Query: 121 ESTFYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRC 180
            + + DQ  YF FG+  Y + ++  IL+   + PNG  Y R  V   I + TGK PA+RC
Sbjct: 121 YA-YLDQFHYFDFGLFVYGNYDVLGILNYYNVYPNGNLYARSDVENIIYSATGKWPALRC 180

Query: 181 NYNSRTGASQLHEISLCFKKDASVFEDCPLNANKCGASFVWNSWNT 222
           N N  +G  QLHEISLCF+K     EDCP NA KC   FVWNSW++
Sbjct: 181 NNNFWSGDVQLHEISLCFRKQDFSLEDCPYNAVKCNDYFVWNSWSS 224

BLAST of MC08g1401 vs. ExPASy TrEMBL
Match: A0A6J1DYI5 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025745 PE=3 SV=1)

HSP 1 Score: 183 bits (465), Expect = 1.82e-54
Identity = 101/225 (44.89%), Postives = 133/225 (59.11%), Query Frame = 0

Query: 1   MAQREIIVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYNKPA-CFQAPGNRFSIH 60
           MA+REII  VL +L+ +A  E  + FDY QVV QWQPATC+D   P  C Q P NRFSIH
Sbjct: 1   MAKREII-FVLVLLLFIASSEQAS-FDYYQVVFQWQPATCNDITLPTRCVQNPDNRFSIH 60

Query: 61  GVWPSLYDGYPT-QCSGSPFDRNQISGLQPGISEIWPNVATGDNWL-WQHEWDKHGTCSE 120
           GVWPS   G     C+G+ FD   I  ++ G+++ WP+V    N + W  EW KHGTCSE
Sbjct: 61  GVWPSKTSGSRVGPCNGTAFDPATIVSIRAGLNQNWPDVIRRKNQVFWSDEWRKHGTCSE 120

Query: 121 STFYDQQEYFGFGMDAYIHNNLQVI--LSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVR 180
               +Q +YF FG++ Y      ++  L   +I PNG+ Y +  V  AI +  GK    R
Sbjct: 121 DLL-NQLQYFTFGLNIYHDEGKDIVEKLGKKKIIPNGKKYKKADVEKAISSVIGKRLGFR 180

Query: 181 CNYNSRTGASQLHEISLCFKKDASVFEDCPLNANKCGASFVWNSW 220
           CN + ++   QLHEIS C+ KDAS  +DCP NA  C   F W+S+
Sbjct: 181 CNID-QSNYHQLHEISFCYAKDASTLQDCPSNATNCPTKFKWSSF 221

BLAST of MC08g1401 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 133.3 bits (334), Expect = 2.5e-31
Identity = 78/224 (34.82%), Postives = 119/224 (53.12%), Query Frame = 0

Query: 6   IIVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYNKPACFQ---APGNRFSIHGVW 65
           +I L++ +  + +      +FD+   V QW P +  D  K  C+     P   F IHG+W
Sbjct: 10  LISLLVILPSVFSASSSSEDFDFFYFVQQW-PGSYCDTQKKCCYPNSGKPAADFGIHGLW 69

Query: 66  PSLYDG-YPTQCSGS-PFDRNQISGLQPGISEIWPNVAT---GDNWLWQHEWDKHGTCSE 125
           P+  DG YP+ C  S PFD + IS L   + + WP +A         W+HEW+KHGTCSE
Sbjct: 70  PNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSE 129

Query: 126 STFYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRCN 185
           S   DQ EYF   ++     NL   L+   I P+G++Y+ + +  +I+   G TP V CN
Sbjct: 130 SVI-DQHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECN 189

Query: 186 YNSRTGASQLHEISLCFKKDASVFEDCPLNAN-KCGASFVWNSW 221
            +  +G SQL+++ LC  +  S   +CP+  + KCGA   + S+
Sbjct: 190 RDG-SGNSQLYQVYLCVDRSGSGLIECPVFPHGKCGAEIEFPSF 230

BLAST of MC08g1401 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 129.4 bits (324), Expect = 3.6e-30
Identity = 76/216 (35.19%), Postives = 113/216 (52.31%), Query Frame = 0

Query: 1   MAQREIIVLVLSMLVL--VAICEGLNEFDYLQVVIQWQPATCSDYNKPACFQA---PGNR 60
           M  +  + ++L +LV   + +     +FD+   V+QW  A C D ++  C+     P   
Sbjct: 1   MGAKGCVNVLLKLLVFQGLFVSRPQEDFDFFYFVLQWPGAYC-DTSRACCYPTSGKPAAD 60

Query: 61  FSIHGVWPSLYDG-YPTQCS-GSPFDRNQISGLQPGISEIWPNVATGDNW---LWQHEWD 120
           F IHG+WP+   G +P+ C   S FDR+QIS L   + + WP ++   N     W+HEW+
Sbjct: 61  FGIHGLWPNYNGGSWPSNCDPDSQFDRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWE 120

Query: 121 KHGTCSESTFYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGK 180
           KHGTCSES   DQ EYF   +      NL  IL N+ I P+   Y  D++  AI+   G 
Sbjct: 121 KHGTCSESVM-DQHEYFENALKLKQKANLLQILKNSGINPDDGFYNLDKITNAIKDGIGF 180

Query: 181 TPAVRCNYNSRTGASQLHEISLCFKKDASVFEDCPL 207
           TP + CN +    A QLH+I +C     + F +CP+
Sbjct: 181 TPGIECNKDPERNA-QLHQIYICVDTSGTEFIECPV 213

BLAST of MC08g1401 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 114.0 bits (284), Expect = 1.6e-25
Identity = 71/209 (33.97%), Postives = 104/209 (49.76%), Query Frame = 0

Query: 6   IIVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYNKPACFQ---APGNRFSIHGVW 65
           I +L L  L + +  +   +FD+   V+QW  A C D     C+     P   F IHG+W
Sbjct: 5   IFILALQQLYVQSFAQ---DFDFFYFVLQWPGAYC-DSRHSCCYPQTGKPAADFGIHGLW 64

Query: 66  PSL-YDGYPTQCS-GSPFDRNQISGLQPGISEIWPNVATGDN---WLWQHEWDKHGTCSE 125
           P+    G+P  C+  S FD  ++S L   +   WP ++   N     W HEW+KHGTC+E
Sbjct: 65  PNYKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAE 124

Query: 126 STFYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRCN 185
           S   DQ +YF  G+      NL   L+N  I P+ + Y    +   I+   G  P + CN
Sbjct: 125 SEL-DQHDYFEAGLKLKQKANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECN 184

Query: 186 YNSRTGASQLHEISLCFKKDASVFEDCPL 207
            +S +  SQL++I LC    AS F +CP+
Sbjct: 185 KDS-SHNSQLYQIYLCVDTSASKFINCPV 207

BLAST of MC08g1401 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 105.9 bits (263), Expect = 4.3e-23
Identity = 67/220 (30.45%), Postives = 105/220 (47.73%), Query Frame = 0

Query: 1   MAQREIIVLVLSMLV------LVAICEGLNEFDYLQVVIQWQPATCSD----YNKPACFQ 60
           MA R  ++L+++ +       ++ +     EFDY  + +QW    C       +K AC +
Sbjct: 1   MASRLCLLLLVACIAGAFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCR 60

Query: 61  ---APGNRFSIHGVWPSLYDG-YPTQCSGSPFDRNQISGLQPGISEIWPNVATGD----- 120
              AP  +F+IHG+WP   DG +P+ C  S F   +IS L  G+ + WP+++ G      
Sbjct: 61  GSDAP-TQFTIHGLWPDYNDGSWPSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCN 120

Query: 121 ---NWLWQHEWDKHGTCSESTFYDQQEYFGFGMDAYI-HNNLQVILSNNQITPNGRTYTR 180
                 W HEW+KHGTCS   F+D+  YF   ++ Y+ HN   V+     +  N   Y  
Sbjct: 121 GGKGSFWGHEWEKHGTCSSPVFHDEYNYFLTTLNLYLKHNVTDVLYQAGYVASNSEKYPL 180

Query: 181 DQVYAAIRAKTGKTPAVRCNYNSRTGASQLHEISLCFKKD 198
             +  AI+     TP V C  ++      + EI +CF KD
Sbjct: 181 GGIVTAIQNAFHITPEVVCKRDA------IDEIRICFYKD 213

BLAST of MC08g1401 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 102.8 bits (255), Expect = 3.6e-22
Identity = 71/221 (32.13%), Postives = 108/221 (48.87%), Query Frame = 0

Query: 8   VLVLSMLVL----VAICEGLNEFDYLQVVIQWQPATCSDYNKPACFQAPGNR---FSIHG 67
           ++++S+L+L    V+  +   +F++   V  W  A C D  K  C    GN    F IHG
Sbjct: 4   IIIVSLLILQSLVVSSSQTEPDFNFFYWVNYWPGAIC-DSQKGCCPPTKGNTASDFIIHG 63

Query: 68  VWPSLYDG-YPTQCSGSP-FDRNQISGLQPGISEIWPN-----VATGDNWLWQHEWDKHG 127
           +WP   +G +P  C  +  FD ++IS L   + + W         + +  LW+HEW+KHG
Sbjct: 64  LWPQFNNGTWPAFCDQTNLFDISKISDLVCQMEKKWTEWGVWACPSNETNLWEHEWNKHG 123

Query: 128 TCSESTFYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPA 187
           TC +S F DQ  YF   +      +L  IL    I PN   Y+ D++  AI+   G  P 
Sbjct: 124 TCVQSIF-DQHSYFRTNLKFKHKVHLLNILIQKGIKPNDGFYSLDEIKNAIKCAIGFAPG 183

Query: 188 VRCNYNSRTGASQLHEISLCFKKDASVFEDCPLNANKCGAS 215
           + CN + + G  QL +I +C    A  F +CP   +K  AS
Sbjct: 184 IECNEDVK-GNKQLFQIYICLDNYAKEFVECPYVPDKSCAS 221

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P235405.3e-3440.82Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1[more]
P800223.5e-3034.51Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2[more]
P428133.5e-3034.82Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1[more]
Q409661.3e-2433.50Ribonuclease S-4 OS=Pyrus pyrifolia OX=3767 PE=1 SV=2[more]
P428152.2e-2433.97Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022149793.13.80e-17299.56ribonuclease MC-like [Momordica charantia][more]
AEM42996.18.69e-11971.74S-RNase [Siraitia grosvenorii][more]
XP_038903744.12.63e-11367.69ribonuclease MC-like [Benincasa hispida][more]
XP_022158939.12.95e-7452.68ribonuclease MC-like [Momordica charantia][more]
XP_022156540.11.94e-6550.44ribonuclease MC-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A6J1D7Q51.84e-17299.56ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111018138 PE=3 SV=1[more]
K7NBR44.21e-11971.74S-RNase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1[more]
A0A6J1E0X11.43e-7452.68ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1[more]
A0A6J1DQK29.38e-6650.44ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111023418 PE=3 SV=1[more]
A0A6J1DYI51.82e-5444.89ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025745 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G02990.12.5e-3134.82ribonuclease 1 [more]
AT1G14220.13.6e-3035.19Ribonuclease T2 family protein [more]
AT1G26820.11.6e-2533.97ribonuclease 3 [more]
AT2G39780.14.3e-2330.45ribonuclease 2 [more]
AT1G14210.13.6e-2232.13Ribonuclease T2 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036430Ribonuclease T2-like superfamilyGENE3D3.90.730.10coord: 26..215
e-value: 3.2E-54
score: 185.8
IPR036430Ribonuclease T2-like superfamilySUPERFAMILY55895Ribonuclease Rh-likecoord: 26..216
IPR001568Ribonuclease T2-likePFAMPF00445Ribonuclease_T2coord: 26..204
e-value: 5.9E-42
score: 143.9
IPR001568Ribonuclease T2-likePANTHERPTHR11240RIBONUCLEASE T2coord: 12..216
NoneNo IPR availablePANTHERPTHR11240:SF49RIBONUCLEASE 3coord: 12..216
IPR033130Ribonuclease T2, His active site 2PROSITEPS00531RNASE_T2_2coord: 104..115
IPR033697Ribonuclease T2, eukaryoticCDDcd01061RNase_T2_eukcoord: 26..205
e-value: 6.18905E-59
score: 182.532

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g1401.1MC08g1401.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function GO:0033897 ribonuclease T2 activity
molecular_function GO:0003723 RNA binding