MC08g0683 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g0683
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Pectinesterase
Location	MC08: 5514635 .. 5517319 (-)
RNA-Seq Expression	MC08g0683
Synteny	MC08g0683

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCAGCGTTTTCGTGGCGGTCTTCGTGGCGGAGATTACGAAGGCGGCGGCGACCGCGGCGATTCTGATCACCGTCGATCAATCCGGTAGAGGAAATTTTACGAAGATTCAAGACGCAATCGACGCGGTGCCGGTTAATAACGTAGAACAGGTGTTTATCGCCGTGAAGCCGGGGATTTACAGAGAGAAGGTGATCGTTCCGGCGAACAAACCGTTTATCACGATCAGCGGAAGAAGAGCAGTTGATACGATCGTGACTTGGAACGATAGCAGAATCACTTATAATTCTGCAACTCTCGCCGTTTTGGCTTCCGATTTCGTCGGACGATACCTCACAATTCAGGTTCTTACTTTAGACGGGAATTTTTTTTTTTAAGTTATATATATGTGTGTGTATATTTTAGGTTTAAATATGGATTTCTTCTCTGTTTTGTTTTTGACTCTTTAAATTCTAACATTTTTAGTTCAAATATTTTGGCTCTTTGAATTCTTACATTTTGATTTCTATGGCTTCATTTACTAAACTTTATTTTCGGTATTTATCTTCTTTATCAACCCTTTCTCTCTCTCTCTAAAAAAAATTTGTTCTATTTTTACAGTGGTTCATTTTCTATCTTATATTTTTATAACCTTCTTAGTTTAGATTCTATTTTAGTAATCTAAATCTAAAAAATTCATTTTGATCTTATAATTTTAACTAAATTGATCATTTCATTAAAAGTATAGAAATTAAAATAGATGCTTTCTAGGTATAAAAACAAAAAATAACAAAAAAAAAATTATTAAAATAATATATAAATATATTTTCTTTAATTTAACGAATATTTTTAAAACGTTCCTTCTTTTTAAGTATTACAAGGCAAAAGAAAATGTCATCGTATGAGTAAGATTTTTCAGCCCTCGTCAGCTGATTAAAACATATTTGTCAGAGGTTGGAGATTTAATTTTTTCTCTCATATTTGTTTAGTAAAAAGCAAGAATTTTCAAAATAGAAAACAAAGGCGAAAAACATTATTTATCATCGTTTGGTTTTTGTCTTATTTTATAATGATTTAATTTTTAATTTTAAATTTTAAAAAACAAAGTTTGTTCCTAACAGCCTCTTTGTCCCGGGCATTTTTCACTTTTTGAAAACTCCTTTCACGCCTTAACAAAATTCCAAAAATTGAGAACATGTAACGAAAGTTATTATTTTTAGTTTTTAAAATATACTACAAATGGAAAAAAAAAGTATTTCTTCATAAGCCTAATACCAAAAAATGATAAGCTAAAGGTAAAAAAGAAAAATAATTCCAATAGTCCTAAATTTTTATTCACTTCAATTTGCTAGGTCAAAATTAGACTTTACTCATTAATAAGAATATAACATATGTATTGTTTTTTTTTTTAGAAATAACATATGTATTGTTATTACATAAACAAAATTTTAAAAAAATGCATTCATTTTCATTTTTTTTTATAAATTAAGAAAATTCACTAAAAAAGTCTCTATTTAAAGATAATGTTATTTATTTATTTATTGATATAAAGAATAATTTGATTGACTTTGTTTGTCGGGTTATTGAAGAACGGGTTCGGGCCGGGTGCGCAAGCGGTGGCGCTACGGGTATCCGGAGACAGGGTGTCGTTCAATGCGTGCAGGTTTTTGGGGCATCAGGACACCCTTCTGGACGACATCGGCCGCCATTACTACAAGAGCTGTTACATCGAAGGAGCCACCGATTTCATTTGCGGAAACGCAGCTTCCCTCTTCGAGGTTCATTTCGTTTCATCTCCTTGTTGGGCCTATTGGGCTGGGATGGAGTTTTAACGTTATTGGGCTTTCTTTTTGTTTTGGGCTTCTACAGAATTGCCATCTGCATTCGGTGTCGGACGCGGTGGGGACCATCACGGCCCAACGGAGGGAGTCCCCGTCGGAGACTACGGGGTTCAGTTTCATGGGGTGCAAGATTACGGGCATAAACTCCGCCGTTTTGGGAAGGCCGTGGGGGGCCTACTCTAGGGTCGTGTTTGGATACACGTATATGTCTGATCTTATTCTGCCCCAGGGCTGGGATGACTGGCAAGACCCATCCAAACACAGGTTCCTTTTTTAGTTTTGTAAATAATTAAAATTAAAAGTTAATTCATCAACTAATTAAACTTGTGAAATTGGGCAGCACGGTGTACTATGGGCAATACAGATGTTATGGGAAAGGAGCCAATCCGTCCAGAAGGGTACCATGGTCATTAACCAACATGACCAGCCAAGAAGCTGCACCATTCTTCAACAAGAGCTTCATCGGCGCCGCCGATTGGCTCCGGCCGCTCCCAACCACTTTCAAGAGAGCCTTCTCTTCCAAACCTTAACCAAAAATAATTTAAAACAAATTAAATTCCCCCCCTTTTTTTACATACATATATTCACAATTCAATCTCAATACTCCAACGTAAACTTAATAAATCAATAAATATTACATCATTCTTTCCTACCTTATTGGCTCTAGTTCTTGTCAAAGTCAACAATATCCTAAGTACTCTATATAACTCTATGATATTTTTCGAATTTTACTTTTGATTTCACTCCAAAGTGTTAAGCATCCCACATTGAAAAGAAAAGGGACCTAACAAACAATGATTTATAATAGACATAAGCTACTGCTCTCATTGCCAATTGATTTTGAGATGAAACCTTATTAATATTGAT

mRNA sequence

ATCAGCGTTTTCGTGGCGGTCTTCGTGGCGGAGATTACGAAGGCGGCGGCGACCGCGGCGATTCTGATCACCGTCGATCAATCCGGTAGAGGAAATTTTACGAAGATTCAAGACGCAATCGACGCGGTGCCGGTTAATAACGTAGAACAGGTGTTTATCGCCGTGAAGCCGGGGATTTACAGAGAGAAGGTGATCGTTCCGGCGAACAAACCGTTTATCACGATCAGCGGAAGAAGAGCAGTTGATACGATCGTGACTTGGAACGATAGCAGAATCACTTATAATTCTGCAACTCTCGCCGTTTTGGCTTCCGATTTCGTCGGACGATACCTCACAATTCAGAACGGGTTCGGGCCGGGTGCGCAAGCGGTGGCGCTACGGGTATCCGGAGACAGGGTGTCGTTCAATGCGTGCAGGTTTTTGGGGCATCAGGACACCCTTCTGGACGACATCGGCCGCCATTACTACAAGAGCTGTTACATCGAAGGAGCCACCGATTTCATTTGCGGAAACGCAGCTTCCCTCTTCGAGAATTGCCATCTGCATTCGGTGTCGGACGCGGTGGGGACCATCACGGCCCAACGGAGGGAGTCCCCGTCGGAGACTACGGGGTTCAGTTTCATGGGGTGCAAGATTACGGGCATAAACTCCGCCGTTTTGGGAAGGCCGTGGGGGGCCTACTCTAGGGTCGTGTTTGGATACACGTATATGTCTGATCTTATTCTGCCCCAGGGCTGGGATGACTGGCAAGACCCATCCAAACACAGCACGGTGTACTATGGGCAATACAGATGTTATGGGAAAGGAGCCAATCCGTCCAGAAGGGTACCATGGTCATTAACCAACATGACCAGCCAAGAAGCTGCACCATTCTTCAACAAGAGCTTCATCGGCGCCGCCGATTGGCTCCGGCCGCTCCCAACCACTTTCAAGAGAGCCTTCTCTTCCAAACCTTAACCAAAAATAATTTAAAACAAATTAAATTCCCCCCCTTTTTTTACATACATATATTCACAATTCAATCTCAATACTCCAACGTAAACTTAATAAATCAATAAATATTACATCATTCTTTCCTACCTTATTGGCTCTAGTTCTTGTCAAAGTCAACAATATCCTAAGTACTCTATATAACTCTATGATATTTTTCGAATTTTACTTTTGATTTCACTCCAAAGTGTTAAGCATCCCACATTGAAAAGAAAAGGGACCTAACAAACAATGATTTATAATAGACATAAGCTACTGCTCTCATTGCCAATTGATTTTGAGATGAAACCTTATTAATATTGAT

Coding sequence (CDS)

ATCAGCGTTTTCGTGGCGGTCTTCGTGGCGGAGATTACGAAGGCGGCGGCGACCGCGGCGATTCTGATCACCGTCGATCAATCCGGTAGAGGAAATTTTACGAAGATTCAAGACGCAATCGACGCGGTGCCGGTTAATAACGTAGAACAGGTGTTTATCGCCGTGAAGCCGGGGATTTACAGAGAGAAGGTGATCGTTCCGGCGAACAAACCGTTTATCACGATCAGCGGAAGAAGAGCAGTTGATACGATCGTGACTTGGAACGATAGCAGAATCACTTATAATTCTGCAACTCTCGCCGTTTTGGCTTCCGATTTCGTCGGACGATACCTCACAATTCAGAACGGGTTCGGGCCGGGTGCGCAAGCGGTGGCGCTACGGGTATCCGGAGACAGGGTGTCGTTCAATGCGTGCAGGTTTTTGGGGCATCAGGACACCCTTCTGGACGACATCGGCCGCCATTACTACAAGAGCTGTTACATCGAAGGAGCCACCGATTTCATTTGCGGAAACGCAGCTTCCCTCTTCGAGAATTGCCATCTGCATTCGGTGTCGGACGCGGTGGGGACCATCACGGCCCAACGGAGGGAGTCCCCGTCGGAGACTACGGGGTTCAGTTTCATGGGGTGCAAGATTACGGGCATAAACTCCGCCGTTTTGGGAAGGCCGTGGGGGGCCTACTCTAGGGTCGTGTTTGGATACACGTATATGTCTGATCTTATTCTGCCCCAGGGCTGGGATGACTGGCAAGACCCATCCAAACACAGCACGGTGTACTATGGGCAATACAGATGTTATGGGAAAGGAGCCAATCCGTCCAGAAGGGTACCATGGTCATTAACCAACATGACCAGCCAAGAAGCTGCACCATTCTTCAACAAGAGCTTCATCGGCGCCGCCGATTGGCTCCGGCCGCTCCCAACCACTTTCAAGAGAGCCTTCTCTTCCAAACCTTAA

Protein sequence

ISVFVAVFVAEITKAAATAAILITVDQSGRGNFTKIQDAIDAVPVNNVEQVFIAVKPGIYREKVIVPANKPFITISGRRAVDTIVTWNDSRITYNSATLAVLASDFVGRYLTIQNGFGPGAQAVALRVSGDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNAASLFENCHLHSVSDAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWGAYSRVVFGYTYMSDLILPQGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTSQEAAPFFNKSFIGAADWLRPLPTTFKRAFSSKP

Homology

[Close]

BLAST of MC08g0683 vs. ExPASy Swiss-Prot
Match: Q9SIJ9 (Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 2.0e-100
Identity = 174/323 (53.87%), Postives = 236/323 (73.07%), Query Frame = 0


Query: 1   ISVFVAVFVAEITKAAATA--------AILITVDQSGRGNFTKIQDAIDAVP--VNNVEQ 60
           I++F+   +   + A++++        AILI VDQSG+G+F+KIQ+AI+++P  +NN + 
Sbjct: 21  INIFILSSITSSSMASSSSPSSIDFSTAILIRVDQSGKGDFSKIQEAIESIPPNLNNSQL 80

Query: 61  VFIAVKPGIYREKVIVPANKPFITISGRRAVDTIVTWNDSRITYNSATLAVLASDFVGRY 120
            FI VKPGIYREKV++PA KP+IT+SG +A +T + W+D      S TL + ASDFV R+
Sbjct: 81  YFIWVKPGIYREKVVIPAEKPYITLSGTQASNTFLIWSDGEDILESPTLTIFASDFVCRF 140

Query: 121 LTIQNGFGPGAQAVALRVSGDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICG 180
           LTIQN FG   +AVALRV+ D+ +F  C    +QDTLLDD G HY+K+CYIEGATDFICG
Sbjct: 141 LTIQNKFGTAGRAVALRVAADKAAFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICG 200

Query: 181 NAASLFENCHLHSVSDAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWGAYSRV 240
           +A+SL+E CHLHS+S   G+ITAQ R S +E +GF+F+GCK+TG  S  LGRPWGAYSRV
Sbjct: 201 SASSLYERCHLHSLSPNNGSITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRV 260

Query: 241 VFGYTYMSDLILPQGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTSQEAAP 300
           VF Y++ S+++ PQGW+ W D +K +TVYYG+Y+CYG GA+  +RV WS   ++ +EA  
Sbjct: 261 VFAYSFFSNVVAPQGWNQWGDSTKENTVYYGEYKCYGPGADREQRVEWS-KQLSDEEATV 320

Query: 301 FFNKSFIGAADWLRPLPTTFKRA 314
           F +K FIG  DWLRP P+ FK A
Sbjct: 321 FLSKDFIGGKDWLRPAPSHFKNA 342

[Close]

BLAST of MC08g0683 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 1.6e-81
Identity = 151/294 (51.36%), Postives = 196/294 (66.67%), Query Frame = 0


Query: 27  QSGRGNFTKIQDAIDAVPVNNVEQVFIAVKPGIYREKVIVPANKPFITISGRRAVDTIVT 86
           +S +G+FTKIQDAID++P+ N  +V I V  G+Y+EKV +P  K FITI G  A  T V 
Sbjct: 90  KSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVE 149

Query: 87  WNDSRI----------TYNSATLAVLASDFVGRYLTIQNGFG---PGA---QAVALRVSG 146
           W D+            TYNSA+ AV +  FV + +T +N      PGA   QAVALRVS 
Sbjct: 150 WGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSA 209

Query: 147 DRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNAASLFENCHLHSVSDAVGT 206
           D  +F  CR LG QDTL D +GRHYYK CYIEG+ DFI GNA SL+E CH+H+++D +G 
Sbjct: 210 DNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGA 269

Query: 207 ITAQRRESPSETTGFSFMGCKITGINSAVLGRPWGAYSRVVFGYTYMSDLILPQGWDDWQ 266
           +TAQ R S  E TGFSF+ CK+TG     LGR WG +SRVVF YTYM ++ILP+GW +W 
Sbjct: 270 VTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWG 329

Query: 267 DPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTSQEAAPFFNKSFIGAADWLR 305
           DPS+  TV+YGQY+C G GAN   RV W+   +T +EA PF + +FI  ++W++
Sbjct: 330 DPSREMTVFYGQYKCTGAGANYGGRVAWA-RELTDEEAKPFLSLTFIDGSEWIK 382

[Close]

BLAST of MC08g0683 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 3.7e-70
Identity = 129/301 (42.86%), Postives = 176/301 (58.47%), Query Frame = 0


Query: 22  LITVDQSGRGNFTKIQDAIDAVPVNNVEQVFIAVKPGIYREKVIVPANKPFITISGRRAV 81
           +ITV  +G   F  +QDA+D++P NN + + I + PG YREKV+VPA KP+IT  G    
Sbjct: 60  VITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRD 119

Query: 82  DTIVTWNDSRI----------TYNSATLAVLASDFVGRYLTIQNGFG------PGAQAVA 141
            T + W+D             TY +A++ V A+ F  R ++  N          G QAVA
Sbjct: 120 VTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVA 179

Query: 142 LRVSGDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNAASLFENCHLHSVS 201
            R+SGD+  F+ C F G QDTL DD GRHY+K CYIEG+ DFI GN  S++++C LHS++
Sbjct: 180 FRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIA 239

Query: 202 DAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWGAYSRVVFGYTYMSDLILPQG 261
              G+I A  R  P E TGF+F+GC++TG     +GR  G YSR+V+ YTY   L+   G
Sbjct: 240 SRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGG 299

Query: 262 WDDWQDPS-KHSTVYYGQYRCYGKGANPSRRVPWSLTNMTSQEAAPFFNKSFIGAADWLR 306
           WDDW   S K  T ++G Y CYG GA  +R V W+   +  + A PF  KSF+    W+ 
Sbjct: 300 WDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARA-LDYESAHPFIAKSFVNGRHWIA 359

[Close]

BLAST of MC08g0683 vs. ExPASy Swiss-Prot
Match: Q9FM79 (Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 2.7e-68
Identity = 129/300 (43.00%), Postives = 179/300 (59.67%), Query Frame = 0


Query: 22  LITVDQSGRGNFTKIQDAIDAVPVNNVEQVFIAVKPGIYREKVIVPANKPFITISGRR-- 81
           +I VD++G G+   +Q A+D VP +N ++V I + PGIYREKVIVP +KP+I+  G    
Sbjct: 81  VIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNESY 140

Query: 82  AVDTIVTWNDSRI----------TYNSATLAVLASDFVGRYLTIQN-----GFGPGAQAV 141
           A DT+++W+D             TY +A++++ +  F    +T +N         G QAV
Sbjct: 141 AGDTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTVVAEAGEQGRQAV 200

Query: 142 ALRVSGDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNAASLFENCHLHSV 201
           ALR+ GD+  F   R LG QDTL DD G HY+  CYI+G  DFI GNA SL+++C +HS 
Sbjct: 201 ALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHST 260

Query: 202 SDAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWGAYSRVVFGYTYMSDLILPQ 261
           +   G I A  R+S +E TGFSF+ C I+G     LGR WG YSR V+   +++D+I P 
Sbjct: 261 AKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITPV 320

Query: 262 GWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTSQEAAPFFNKSFIGAADWLR 305
           GW DW+ P +   V +G+Y C G+GA    RVPWS T +T  E  PF  + FI    WLR
Sbjct: 321 GWSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKT-LTRDEVKPFLGREFIYGDQWLR 379

[Close]

BLAST of MC08g0683 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 3.0e-67
Identity = 133/300 (44.33%), Postives = 187/300 (62.33%), Query Frame = 0


Query: 20  AILITVDQSGRGNFTKIQDAIDAVPVNNVEQVFIAVKPGIYREKVIVPANKPFITISGRR 79
           ++++TVD  G GNF+ +Q AID VP  +  +  I V  G YREKV V  NK  + I GR 
Sbjct: 88  SLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGRG 147

Query: 80  AVDTIVTWNDSR----ITYNSATLAVLASDFVGRYLTIQNGF---GPG---AQAVALRVS 139
             +T + WND+      T +S +  V A++F    ++ +N      PG   AQAVALR+ 
Sbjct: 148 YQNTSIEWNDTAKSAGNTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQAVALRIE 207

Query: 140 GDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNAASLFENCHLHSV----- 199
           GD+ +F  C F G QDTLLDD GRH++K C+I+G+ DFI GN  SL+++C ++S+     
Sbjct: 208 GDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTINSIAKGNT 267

Query: 200 SDAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWGAYSRVVFGYTYMSDLILPQ 259
           S   G+ITAQ R+S  E +GFSF+ CKI G    +LGR WGAY+ VVF  TYMS +I P+
Sbjct: 268 SGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIITPE 327

Query: 260 GWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTSQEAAPFFNKSFIGAADWLR 305
           GW++W D +K  TV +G+++CYG GA+   RV +    +T  EA+ F + SFI   +WLR
Sbjct: 328 GWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFG-KQLTDSEASSFIDVSFIDGDEWLR 386

[Close]

BLAST of MC08g0683 vs. NCBI nr
Match: XP_022144190.1 (putative pectinesterase 11 [Momordica charantia])

HSP 1 Score: 642 bits (1656), Expect = 3.72e-232
Identity = 318/318 (100.00%), Postives = 318/318 (100.00%), Query Frame = 0


Query: 1   ISVFVAVFVAEITKAAATAAILITVDQSGRGNFTKIQDAIDAVPVNNVEQVFIAVKPGIY 60
           ISVFVAVFVAEITKAAATAAILITVDQSGRGNFTKIQDAIDAVPVNNVEQVFIAVKPGIY
Sbjct: 16  ISVFVAVFVAEITKAAATAAILITVDQSGRGNFTKIQDAIDAVPVNNVEQVFIAVKPGIY 75

Query: 61  REKVIVPANKPFITISGRRAVDTIVTWNDSRITYNSATLAVLASDFVGRYLTIQNGFGPG 120
           REKVIVPANKPFITISGRRAVDTIVTWNDSRITYNSATLAVLASDFVGRYLTIQNGFGPG
Sbjct: 76  REKVIVPANKPFITISGRRAVDTIVTWNDSRITYNSATLAVLASDFVGRYLTIQNGFGPG 135

Query: 121 AQAVALRVSGDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNAASLFENCH 180
           AQAVALRVSGDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNAASLFENCH
Sbjct: 136 AQAVALRVSGDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNAASLFENCH 195

Query: 181 LHSVSDAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWGAYSRVVFGYTYMSDL 240
           LHSVSDAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWGAYSRVVFGYTYMSDL
Sbjct: 196 LHSVSDAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWGAYSRVVFGYTYMSDL 255

Query: 241 ILPQGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTSQEAAPFFNKSFIGAA 300
           ILPQGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTSQEAAPFFNKSFIGAA
Sbjct: 256 ILPQGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTSQEAAPFFNKSFIGAA 315

Query: 301 DWLRPLPTTFKRAFSSKP 318
           DWLRPLPTTFKRAFSSKP
Sbjct: 316 DWLRPLPTTFKRAFSSKP 333

[Close]

BLAST of MC08g0683 vs. NCBI nr
Match: XP_023536774.1 (putative pectinesterase 11 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 572 bits (1475), Expect = 1.23e-204
Identity = 277/317 (87.38%), Postives = 296/317 (93.38%), Query Frame = 0


Query: 2   SVFVAVFVAEITKAAATAAILITVDQSGRGNFTKIQDAIDAVPVNNVEQVFIAVKPGIYR 61
           ++ + V VAE T A A  AILITVDQSGRGNFTKIQ+AIDAVP NN+E+VFIAVKPGIYR
Sbjct: 13  AIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPENNLEEVFIAVKPGIYR 72

Query: 62  EKVIVPANKPFITISGRRAVDTIVTWNDSRITYNSATLAVLASDFVGRYLTIQNGFGPGA 121
           EKV VPANKP+ITISGRR+ DTI+TWNDSR TYNSATLAVLASDFVGRYLTIQNGFG GA
Sbjct: 73  EKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGRYLTIQNGFGAGA 132

Query: 122 QAVALRVSGDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNAASLFENCHL 181
           QAVALRVSGDRVSF ACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGN ASLFENCHL
Sbjct: 133 QAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNGASLFENCHL 192

Query: 182 HSVSDAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWGAYSRVVFGYTYMSDLI 241
           HSVSD+VGTITAQRRESPSE TGF FMGCKITGINSAVLGRPWGA+SRVVFG+TYMSD+I
Sbjct: 193 HSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFTYMSDVI 252

Query: 242 LPQGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTSQEAAPFFNKSFIGAAD 301
           LP+GWD+WQDPSK STVYYGQYRCYGKGANPSRRVPWS TNMT+QEAAPFF+K+FIGAA 
Sbjct: 253 LPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFTNMTTQEAAPFFSKTFIGAAH 312

Query: 302 WLRPLPTTFKRAFSSKP 318
           WLRPLPT FKRAF+SKP
Sbjct: 313 WLRPLPTNFKRAFASKP 329

[Close]

BLAST of MC08g0683 vs. NCBI nr
Match: XP_022951672.1 (putative pectinesterase 11 [Cucurbita moschata])

HSP 1 Score: 571 bits (1472), Expect = 3.54e-204
Identity = 276/317 (87.07%), Postives = 296/317 (93.38%), Query Frame = 0


Query: 2   SVFVAVFVAEITKAAATAAILITVDQSGRGNFTKIQDAIDAVPVNNVEQVFIAVKPGIYR 61
           ++ + V VAE T A A  AILITVDQSGRGNFTKIQ+AIDAVP NN+E+VFIAVKPGIYR
Sbjct: 13  AIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPENNLEEVFIAVKPGIYR 72

Query: 62  EKVIVPANKPFITISGRRAVDTIVTWNDSRITYNSATLAVLASDFVGRYLTIQNGFGPGA 121
           EKV VPANKP+ITISGRR+ DTI+TWNDSR TYNSATLAVLASDFVGRYLTIQNGFG GA
Sbjct: 73  EKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGRYLTIQNGFGAGA 132

Query: 122 QAVALRVSGDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNAASLFENCHL 181
           QAVALRVSGDRVSF ACRFLGHQDTLLDDIGRHYY+SCYIEGATDFICGN ASLFENCHL
Sbjct: 133 QAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYRSCYIEGATDFICGNGASLFENCHL 192

Query: 182 HSVSDAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWGAYSRVVFGYTYMSDLI 241
           HSVSD+VGTITAQRRESPSE TGF FMGCKITGINSAVLGRPWGA+SRVVFG+TYMSD+I
Sbjct: 193 HSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFTYMSDVI 252

Query: 242 LPQGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTSQEAAPFFNKSFIGAAD 301
           LP+GWD+WQDPSK STVYYGQYRCYGKGANPSRRVPWS TNMT+QEAAPFF+K+FIGAA 
Sbjct: 253 LPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFTNMTTQEAAPFFSKTFIGAAH 312

Query: 302 WLRPLPTTFKRAFSSKP 318
           WLRPLPT FKRAF+SKP
Sbjct: 313 WLRPLPTNFKRAFASKP 329

[Close]

BLAST of MC08g0683 vs. NCBI nr
Match: XP_023002424.1 (putative pectinesterase 11 [Cucurbita maxima])

HSP 1 Score: 570 bits (1470), Expect = 7.13e-204
Identity = 276/317 (87.07%), Postives = 296/317 (93.38%), Query Frame = 0


Query: 2   SVFVAVFVAEITKAAATAAILITVDQSGRGNFTKIQDAIDAVPVNNVEQVFIAVKPGIYR 61
           ++ + V VAE T A A  AILITVDQSGRGNFTKIQ+AIDAVP NN+E+VFIAVKPGIYR
Sbjct: 13  AIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPDNNLEEVFIAVKPGIYR 72

Query: 62  EKVIVPANKPFITISGRRAVDTIVTWNDSRITYNSATLAVLASDFVGRYLTIQNGFGPGA 121
           EKV VPANKP+ITISGRR+ DTI+TWNDSR TYNSATLAVLASDFVGRYLTIQNGFG GA
Sbjct: 73  EKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGRYLTIQNGFGAGA 132

Query: 122 QAVALRVSGDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNAASLFENCHL 181
           QAVALRVSGDRVSF ACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGN ASLFENCHL
Sbjct: 133 QAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNGASLFENCHL 192

Query: 182 HSVSDAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWGAYSRVVFGYTYMSDLI 241
           HSVSD+VGTITAQRRESPSE TGF FMGCKITGINSAVLGRPWGA+SRVVFG+TYMSD+I
Sbjct: 193 HSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFTYMSDVI 252

Query: 242 LPQGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTSQEAAPFFNKSFIGAAD 301
           LP+GWD+WQDPSK STVYYGQYRCYGKGANPSRRVPWS +NMT+QEAAPFF+K+FIGAA 
Sbjct: 253 LPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAAPFFSKTFIGAAH 312

Query: 302 WLRPLPTTFKRAFSSKP 318
           WLRPLPT FKRAF+SKP
Sbjct: 313 WLRPLPTNFKRAFASKP 329

[Close]

BLAST of MC08g0683 vs. NCBI nr
Match: KAG6585588.1 (putative pectinesterase 11, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020503.1 putative pectinesterase 11, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 570 bits (1468), Expect = 1.44e-203
Identity = 275/317 (86.75%), Postives = 295/317 (93.06%), Query Frame = 0


Query: 2   SVFVAVFVAEITKAAATAAILITVDQSGRGNFTKIQDAIDAVPVNNVEQVFIAVKPGIYR 61
           ++ + V VAE T A A  AILITVDQSGRGNFTKIQ+AID VP NN+E+VFIAVKPGIYR
Sbjct: 13  AIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDVVPENNLEEVFIAVKPGIYR 72

Query: 62  EKVIVPANKPFITISGRRAVDTIVTWNDSRITYNSATLAVLASDFVGRYLTIQNGFGPGA 121
           EKV VPANKP+ITISGRR+ DTI+TWNDSR TYNSATLAVLASDFVGRYLTIQNGFG GA
Sbjct: 73  EKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGRYLTIQNGFGAGA 132

Query: 122 QAVALRVSGDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNAASLFENCHL 181
           QAVALRVSGDRVSF ACRFLGHQDTLLDDIGRHYY+SCYIEGATDFICGN ASLFENCHL
Sbjct: 133 QAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYRSCYIEGATDFICGNGASLFENCHL 192

Query: 182 HSVSDAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWGAYSRVVFGYTYMSDLI 241
           HSVSD+VGTITAQRRESPSE TGF FMGCKITGINSAVLGRPWGA+SRVVFG+TYMSD+I
Sbjct: 193 HSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFTYMSDVI 252

Query: 242 LPQGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTSQEAAPFFNKSFIGAAD 301
           LP+GWD+WQDPSK STVYYGQYRCYGKGANPSRRVPWS TNMT+QEAAPFF+K+FIGAA 
Sbjct: 253 LPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFTNMTTQEAAPFFSKTFIGAAH 312

Query: 302 WLRPLPTTFKRAFSSKP 318
           WLRPLPT FKRAF+SKP
Sbjct: 313 WLRPLPTNFKRAFASKP 329

[Close]

BLAST of MC08g0683 vs. ExPASy TrEMBL
Match: A0A6J1CQY5 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111013943 PE=4 SV=1)

HSP 1 Score: 642 bits (1656), Expect = 1.80e-232
Identity = 318/318 (100.00%), Postives = 318/318 (100.00%), Query Frame = 0


Query: 1   ISVFVAVFVAEITKAAATAAILITVDQSGRGNFTKIQDAIDAVPVNNVEQVFIAVKPGIY 60
           ISVFVAVFVAEITKAAATAAILITVDQSGRGNFTKIQDAIDAVPVNNVEQVFIAVKPGIY
Sbjct: 16  ISVFVAVFVAEITKAAATAAILITVDQSGRGNFTKIQDAIDAVPVNNVEQVFIAVKPGIY 75

Query: 61  REKVIVPANKPFITISGRRAVDTIVTWNDSRITYNSATLAVLASDFVGRYLTIQNGFGPG 120
           REKVIVPANKPFITISGRRAVDTIVTWNDSRITYNSATLAVLASDFVGRYLTIQNGFGPG
Sbjct: 76  REKVIVPANKPFITISGRRAVDTIVTWNDSRITYNSATLAVLASDFVGRYLTIQNGFGPG 135

Query: 121 AQAVALRVSGDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNAASLFENCH 180
           AQAVALRVSGDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNAASLFENCH
Sbjct: 136 AQAVALRVSGDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNAASLFENCH 195

Query: 181 LHSVSDAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWGAYSRVVFGYTYMSDL 240
           LHSVSDAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWGAYSRVVFGYTYMSDL
Sbjct: 196 LHSVSDAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWGAYSRVVFGYTYMSDL 255

Query: 241 ILPQGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTSQEAAPFFNKSFIGAA 300
           ILPQGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTSQEAAPFFNKSFIGAA
Sbjct: 256 ILPQGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTSQEAAPFFNKSFIGAA 315

Query: 301 DWLRPLPTTFKRAFSSKP 318
           DWLRPLPTTFKRAFSSKP
Sbjct: 316 DWLRPLPTTFKRAFSSKP 333

[Close]

BLAST of MC08g0683 vs. ExPASy TrEMBL
Match: A0A6J1GIB5 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111454415 PE=4 SV=1)

HSP 1 Score: 571 bits (1472), Expect = 1.71e-204
Identity = 276/317 (87.07%), Postives = 296/317 (93.38%), Query Frame = 0


Query: 2   SVFVAVFVAEITKAAATAAILITVDQSGRGNFTKIQDAIDAVPVNNVEQVFIAVKPGIYR 61
           ++ + V VAE T A A  AILITVDQSGRGNFTKIQ+AIDAVP NN+E+VFIAVKPGIYR
Sbjct: 13  AIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPENNLEEVFIAVKPGIYR 72

Query: 62  EKVIVPANKPFITISGRRAVDTIVTWNDSRITYNSATLAVLASDFVGRYLTIQNGFGPGA 121
           EKV VPANKP+ITISGRR+ DTI+TWNDSR TYNSATLAVLASDFVGRYLTIQNGFG GA
Sbjct: 73  EKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGRYLTIQNGFGAGA 132

Query: 122 QAVALRVSGDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNAASLFENCHL 181
           QAVALRVSGDRVSF ACRFLGHQDTLLDDIGRHYY+SCYIEGATDFICGN ASLFENCHL
Sbjct: 133 QAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYRSCYIEGATDFICGNGASLFENCHL 192

Query: 182 HSVSDAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWGAYSRVVFGYTYMSDLI 241
           HSVSD+VGTITAQRRESPSE TGF FMGCKITGINSAVLGRPWGA+SRVVFG+TYMSD+I
Sbjct: 193 HSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFTYMSDVI 252

Query: 242 LPQGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTSQEAAPFFNKSFIGAAD 301
           LP+GWD+WQDPSK STVYYGQYRCYGKGANPSRRVPWS TNMT+QEAAPFF+K+FIGAA 
Sbjct: 253 LPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFTNMTTQEAAPFFSKTFIGAAH 312

Query: 302 WLRPLPTTFKRAFSSKP 318
           WLRPLPT FKRAF+SKP
Sbjct: 313 WLRPLPTNFKRAFASKP 329

[Close]

BLAST of MC08g0683 vs. ExPASy TrEMBL
Match: A0A6J1KQF0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496269 PE=4 SV=1)

HSP 1 Score: 570 bits (1470), Expect = 3.45e-204
Identity = 276/317 (87.07%), Postives = 296/317 (93.38%), Query Frame = 0


Query: 2   SVFVAVFVAEITKAAATAAILITVDQSGRGNFTKIQDAIDAVPVNNVEQVFIAVKPGIYR 61
           ++ + V VAE T A A  AILITVDQSGRGNFTKIQ+AIDAVP NN+E+VFIAVKPGIYR
Sbjct: 13  AIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPDNNLEEVFIAVKPGIYR 72

Query: 62  EKVIVPANKPFITISGRRAVDTIVTWNDSRITYNSATLAVLASDFVGRYLTIQNGFGPGA 121
           EKV VPANKP+ITISGRR+ DTI+TWNDSR TYNSATLAVLASDFVGRYLTIQNGFG GA
Sbjct: 73  EKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGRYLTIQNGFGAGA 132

Query: 122 QAVALRVSGDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNAASLFENCHL 181
           QAVALRVSGDRVSF ACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGN ASLFENCHL
Sbjct: 133 QAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNGASLFENCHL 192

Query: 182 HSVSDAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWGAYSRVVFGYTYMSDLI 241
           HSVSD+VGTITAQRRESPSE TGF FMGCKITGINSAVLGRPWGA+SRVVFG+TYMSD+I
Sbjct: 193 HSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFTYMSDVI 252

Query: 242 LPQGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTSQEAAPFFNKSFIGAAD 301
           LP+GWD+WQDPSK STVYYGQYRCYGKGANPSRRVPWS +NMT+QEAAPFF+K+FIGAA 
Sbjct: 253 LPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAAPFFSKTFIGAAH 312

Query: 302 WLRPLPTTFKRAFSSKP 318
           WLRPLPT FKRAF+SKP
Sbjct: 313 WLRPLPTNFKRAFASKP 329

[Close]

BLAST of MC08g0683 vs. ExPASy TrEMBL
Match: A0A0A0LPB4 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G361760 PE=4 SV=1)

HSP 1 Score: 563 bits (1451), Expect = 2.52e-201
Identity = 272/318 (85.53%), Postives = 295/318 (92.77%), Query Frame = 0


Query: 1   ISVFVAVFVAEITKAAATAAILITVDQSGRGNFTKIQDAIDAVPVNNVEQVFIAVKPGIY 60
           ISV  AV VAE+T AA T A+LITVDQSG+GNFTKIQ AIDAVP+NN E+VFI+VK GIY
Sbjct: 10  ISVIAAVLVAEVTAAAVTTAVLITVDQSGKGNFTKIQQAIDAVPINNKEEVFISVKAGIY 69

Query: 61  REKVIVPANKPFITISGRRAVDTIVTWNDSRITYNSATLAVLASDFVGRYLTIQNGFGPG 120
           REKV+VPANKPFITISGRRAVDTI++WNDS+ TYNSATLAVLASDFVGRYLTIQNG+GPG
Sbjct: 70  REKVVVPANKPFITISGRRAVDTIISWNDSKNTYNSATLAVLASDFVGRYLTIQNGYGPG 129

Query: 121 AQAVALRVSGDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNAASLFENCH 180
           AQAVALRVSGDRVSF ACRFLGHQDTLLDDIGRHYYKSCYI+GATDFICGNAASLFENCH
Sbjct: 130 AQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIQGATDFICGNAASLFENCH 189

Query: 181 LHSVSDAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWGAYSRVVFGYTYMSDL 240
           L SVS+ VGTITAQRRESPSE TGF FMGCKITGINSAVLGRPWGA+SRVVFG+T+MSD+
Sbjct: 190 LRSVSEDVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFTFMSDV 249

Query: 241 ILPQGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTSQEAAPFFNKSFIGAA 300
           ILP+GWD+WQDPSK STVYYGQY+CYGKGAN SRRV WS TNMT+Q+AAPFF KSFIGAA
Sbjct: 250 ILPEGWDNWQDPSKQSTVYYGQYKCYGKGANTSRRVSWSFTNMTAQDAAPFFTKSFIGAA 309

Query: 301 DWLRPLPTTFKRAFSSKP 318
           DWLRP+P  FKRAFSS P
Sbjct: 310 DWLRPVPNRFKRAFSSHP 327

[Close]

BLAST of MC08g0683 vs. ExPASy TrEMBL
Match: A0A1S3BBW3 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103487980 PE=4 SV=1)

HSP 1 Score: 550 bits (1416), Expect = 5.61e-196
Identity = 269/317 (84.86%), Postives = 291/317 (91.80%), Query Frame = 0


Query: 1   ISVFVAVFVAEITKAAATAAILITVDQSGRGNFTKIQDAIDAVPVNNVEQVFIAVKPGIY 60
           +SV  AV VAE+T AA T A+LITVDQSG+GNFTKIQ AIDAVP NN E+VFI VK GIY
Sbjct: 10  VSVIAAVLVAEVT-AAETTAVLITVDQSGKGNFTKIQQAIDAVPENNREEVFIVVKAGIY 69

Query: 61  REKVIVPANKPFITISGRRAVDTIVTWNDSRITYNSATLAVLASDFVGRYLTIQNGFGPG 120
           REKV+VPANKPFITISGRRA DTI++WNDS+ TYNSATLAVLASDFVGRYLTIQN +G G
Sbjct: 70  REKVVVPANKPFITISGRRAADTIISWNDSKNTYNSATLAVLASDFVGRYLTIQNEYGAG 129

Query: 121 AQAVALRVSGDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNAASLFENCH 180
           AQAVALRVSGDRVSF ACRFLGHQDTLLDDIGRHYYKSCYI+GATDFICGNAASLFENCH
Sbjct: 130 AQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIQGATDFICGNAASLFENCH 189

Query: 181 LHSVSDAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWGAYSRVVFGYTYMSDL 240
           L SVSD VGTITAQRRESPSE TGF F+GCKITGINSAVLGRPWGA+SRVVFG+T+MSD+
Sbjct: 190 LRSVSDDVGTITAQRRESPSENTGFVFLGCKITGINSAVLGRPWGAFSRVVFGFTFMSDV 249

Query: 241 ILPQGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTSQEAAPFFNKSFIGAA 300
           ILP+GWD+WQDPSK STVYYGQY+CYGKGANPSRRV WS TNMT+Q+AAPFF KSFIGAA
Sbjct: 250 ILPEGWDNWQDPSKQSTVYYGQYKCYGKGANPSRRVSWSFTNMTAQDAAPFFTKSFIGAA 309

Query: 301 DWLRPLPTTFKRAFSSK 317
           DWLRPLP  FKRAFSS+
Sbjct: 310 DWLRPLPKHFKRAFSSR 325

[Close]

BLAST of MC08g0683 vs. TAIR 10
Match: AT2G21610.1 (pectinesterase 11 )

HSP 1 Score: 367.1 bits (941), Expect = 1.4e-101
Identity = 174/323 (53.87%), Postives = 236/323 (73.07%), Query Frame = 0


Query: 1   ISVFVAVFVAEITKAAATA--------AILITVDQSGRGNFTKIQDAIDAVP--VNNVEQ 60
           I++F+   +   + A++++        AILI VDQSG+G+F+KIQ+AI+++P  +NN + 
Sbjct: 21  INIFILSSITSSSMASSSSPSSIDFSTAILIRVDQSGKGDFSKIQEAIESIPPNLNNSQL 80

Query: 61  VFIAVKPGIYREKVIVPANKPFITISGRRAVDTIVTWNDSRITYNSATLAVLASDFVGRY 120
            FI VKPGIYREKV++PA KP+IT+SG +A +T + W+D      S TL + ASDFV R+
Sbjct: 81  YFIWVKPGIYREKVVIPAEKPYITLSGTQASNTFLIWSDGEDILESPTLTIFASDFVCRF 140

Query: 121 LTIQNGFGPGAQAVALRVSGDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICG 180
           LTIQN FG   +AVALRV+ D+ +F  C    +QDTLLDD G HY+K+CYIEGATDFICG
Sbjct: 141 LTIQNKFGTAGRAVALRVAADKAAFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICG 200

Query: 181 NAASLFENCHLHSVSDAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWGAYSRV 240
           +A+SL+E CHLHS+S   G+ITAQ R S +E +GF+F+GCK+TG  S  LGRPWGAYSRV
Sbjct: 201 SASSLYERCHLHSLSPNNGSITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRV 260

Query: 241 VFGYTYMSDLILPQGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTSQEAAP 300
           VF Y++ S+++ PQGW+ W D +K +TVYYG+Y+CYG GA+  +RV WS   ++ +EA  
Sbjct: 261 VFAYSFFSNVVAPQGWNQWGDSTKENTVYYGEYKCYGPGADREQRVEWS-KQLSDEEATV 320

Query: 301 FFNKSFIGAADWLRPLPTTFKRA 314
           F +K FIG  DWLRP P+ FK A
Sbjct: 321 FLSKDFIGGKDWLRPAPSHFKNA 342

[Close]

BLAST of MC08g0683 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 304.3 bits (778), Expect = 1.2e-82
Identity = 151/294 (51.36%), Postives = 196/294 (66.67%), Query Frame = 0


Query: 27  QSGRGNFTKIQDAIDAVPVNNVEQVFIAVKPGIYREKVIVPANKPFITISGRRAVDTIVT 86
           +S +G+FTKIQDAID++P+ N  +V I V  G+Y+EKV +P  K FITI G  A  T V 
Sbjct: 90  KSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVE 149

Query: 87  WNDSRI----------TYNSATLAVLASDFVGRYLTIQNGFG---PGA---QAVALRVSG 146
           W D+            TYNSA+ AV +  FV + +T +N      PGA   QAVALRVS 
Sbjct: 150 WGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSA 209

Query: 147 DRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNAASLFENCHLHSVSDAVGT 206
           D  +F  CR LG QDTL D +GRHYYK CYIEG+ DFI GNA SL+E CH+H+++D +G 
Sbjct: 210 DNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGA 269

Query: 207 ITAQRRESPSETTGFSFMGCKITGINSAVLGRPWGAYSRVVFGYTYMSDLILPQGWDDWQ 266
           +TAQ R S  E TGFSF+ CK+TG     LGR WG +SRVVF YTYM ++ILP+GW +W 
Sbjct: 270 VTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWG 329

Query: 267 DPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTSQEAAPFFNKSFIGAADWLR 305
           DPS+  TV+YGQY+C G GAN   RV W+   +T +EA PF + +FI  ++W++
Sbjct: 330 DPSREMTVFYGQYKCTGAGANYGGRVAWA-RELTDEEAKPFLSLTFIDGSEWIK 382

[Close]

BLAST of MC08g0683 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 266.5 bits (680), Expect = 2.7e-71
Identity = 129/301 (42.86%), Postives = 176/301 (58.47%), Query Frame = 0


Query: 22  LITVDQSGRGNFTKIQDAIDAVPVNNVEQVFIAVKPGIYREKVIVPANKPFITISGRRAV 81
           +ITV  +G   F  +QDA+D++P NN + + I + PG YREKV+VPA KP+IT  G    
Sbjct: 60  VITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRD 119

Query: 82  DTIVTWNDSRI----------TYNSATLAVLASDFVGRYLTIQNGFG------PGAQAVA 141
            T + W+D             TY +A++ V A+ F  R ++  N          G QAVA
Sbjct: 120 VTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVA 179

Query: 142 LRVSGDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNAASLFENCHLHSVS 201
            R+SGD+  F+ C F G QDTL DD GRHY+K CYIEG+ DFI GN  S++++C LHS++
Sbjct: 180 FRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIA 239

Query: 202 DAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWGAYSRVVFGYTYMSDLILPQG 261
              G+I A  R  P E TGF+F+GC++TG     +GR  G YSR+V+ YTY   L+   G
Sbjct: 240 SRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGG 299

Query: 262 WDDWQDPS-KHSTVYYGQYRCYGKGANPSRRVPWSLTNMTSQEAAPFFNKSFIGAADWLR 306
           WDDW   S K  T ++G Y CYG GA  +R V W+   +  + A PF  KSF+    W+ 
Sbjct: 300 WDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARA-LDYESAHPFIAKSFVNGRHWIA 359

[Close]

BLAST of MC08g0683 vs. TAIR 10
Match: AT5G55590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 260.4 bits (664), Expect = 1.9e-69
Identity = 129/300 (43.00%), Postives = 179/300 (59.67%), Query Frame = 0


Query: 22  LITVDQSGRGNFTKIQDAIDAVPVNNVEQVFIAVKPGIYREKVIVPANKPFITISGRR-- 81
           +I VD++G G+   +Q A+D VP +N ++V I + PGIYREKVIVP +KP+I+  G    
Sbjct: 81  VIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNESY 140

Query: 82  AVDTIVTWNDSRI----------TYNSATLAVLASDFVGRYLTIQN-----GFGPGAQAV 141
           A DT+++W+D             TY +A++++ +  F    +T +N         G QAV
Sbjct: 141 AGDTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTVVAEAGEQGRQAV 200

Query: 142 ALRVSGDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNAASLFENCHLHSV 201
           ALR+ GD+  F   R LG QDTL DD G HY+  CYI+G  DFI GNA SL+++C +HS 
Sbjct: 201 ALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHST 260

Query: 202 SDAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWGAYSRVVFGYTYMSDLILPQ 261
           +   G I A  R+S +E TGFSF+ C I+G     LGR WG YSR V+   +++D+I P 
Sbjct: 261 AKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITPV 320

Query: 262 GWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTSQEAAPFFNKSFIGAADWLR 305
           GW DW+ P +   V +G+Y C G+GA    RVPWS T +T  E  PF  + FI    WLR
Sbjct: 321 GWSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKT-LTRDEVKPFLGREFIYGDQWLR 379

[Close]

BLAST of MC08g0683 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 256.9 bits (655), Expect = 2.1e-68
Identity = 133/300 (44.33%), Postives = 187/300 (62.33%), Query Frame = 0


Query: 20  AILITVDQSGRGNFTKIQDAIDAVPVNNVEQVFIAVKPGIYREKVIVPANKPFITISGRR 79
           ++++TVD  G GNF+ +Q AID VP  +  +  I V  G YREKV V  NK  + I GR 
Sbjct: 88  SLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGRG 147

Query: 80  AVDTIVTWNDSR----ITYNSATLAVLASDFVGRYLTIQNGF---GPG---AQAVALRVS 139
             +T + WND+      T +S +  V A++F    ++ +N      PG   AQAVALR+ 
Sbjct: 148 YQNTSIEWNDTAKSAGNTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQAVALRIE 207

Query: 140 GDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNAASLFENCHLHSV----- 199
           GD+ +F  C F G QDTLLDD GRH++K C+I+G+ DFI GN  SL+++C ++S+     
Sbjct: 208 GDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTINSIAKGNT 267

Query: 200 SDAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWGAYSRVVFGYTYMSDLILPQ 259
           S   G+ITAQ R+S  E +GFSF+ CKI G    +LGR WGAY+ VVF  TYMS +I P+
Sbjct: 268 SGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIITPE 327

Query: 260 GWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTSQEAAPFFNKSFIGAADWLR 305
           GW++W D +K  TV +G+++CYG GA+   RV +    +T  EA+ F + SFI   +WLR
Sbjct: 328 GWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFG-KQLTDSEASSFIDVSFIDGDEWLR 386

The following BLAST results are available for this feature:

BLAST of MC08g0683 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SIJ9	2.0e-100	53.87	Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1	[more]
Q8VYZ3	1.6e-81	51.36	Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1	[more]
Q8LPF3	3.7e-70	42.86	Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1	[more]
Q9FM79	2.7e-68	43.00	Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1	[more]
Q9ZQA3	3.0e-67	44.33	Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1	[more]

BLAST of MC08g0683 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144190.1	3.72e-232	100.00	putative pectinesterase 11 [Momordica charantia]	[more]
XP_023536774.1	1.23e-204	87.38	putative pectinesterase 11 [Cucurbita pepo subsp. pepo]	[more]
XP_022951672.1	3.54e-204	87.07	putative pectinesterase 11 [Cucurbita moschata]	[more]
XP_023002424.1	7.13e-204	87.07	putative pectinesterase 11 [Cucurbita maxima]	[more]
KAG6585588.1	1.44e-203	86.75	putative pectinesterase 11, partial [Cucurbita argyrosperma subsp. sororia] >KAG...	[more]

BLAST of MC08g0683 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CQY5	1.80e-232	100.00	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111013943 PE=4 SV=1	[more]
A0A6J1GIB5	1.71e-204	87.07	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111454415 PE=4 SV=1	[more]
A0A6J1KQF0	3.45e-204	87.07	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496269 PE=4 SV=1	[more]
A0A0A0LPB4	2.52e-201	85.53	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G361760 PE=4 SV=1	[more]
A0A1S3BBW3	5.61e-196	84.86	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103487980 PE=4 SV=1	[more]

BLAST of MC08g0683 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G21610.1	1.4e-101	53.87	pectinesterase 11	[more]
AT5G19730.1	1.2e-82	51.36	Pectin lyase-like superfamily protein	[more]
AT5G47500.1	2.7e-71	42.86	Pectin lyase-like superfamily protein	[more]
AT5G55590.1	1.9e-69	43.00	Pectin lyase-like superfamily protein	[more]
AT2G36710.1	2.1e-68	44.33	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 23..298 e-value: 5.5E-72 score: 242.0
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 18..313 e-value: 3.4E-108 score: 363.8
None	No IPR available	PANTHER	PTHR31321:SF77	PECTINESTERASE 11-RELATED	coord: 11..304
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 11..304
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 16..310

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g0683.1	MC08g0683.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity