MC08g0677 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g0677
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	AMP-binding domain-containing protein
Location	MC08: 5464856 .. 5468597 (+)
RNA-Seq Expression	MC08g0677
Synteny	MC08g0677

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CTACAATTGGTATGTCAATTTAGCATTAACATTTAAAATAAAGGTTGTAACAACGATTTTAGGGTTTATTAAAATTAAAATCAAAGAACATAGCAATAAAAGAAAACCTTGTTAAAAATTCGGGAGCCAATAATGAATCATAAAGGACAGATTTGTAATTTTGGGCATAATTTGTGTGGGTATAAATAGGGGCAGATGCGGCAAATCAGTTGCAAAAATTAAAGCAAAAAAAAAAAAAAACGAAAGAGAGAGTAGAGTATGCTGTTTGAGAATTTTGATCCTTATTTCCCGGACCAGCCGGTGGTTGACCTGTACCTCCCGGTTTGGGCGAACCTCCCGGCGTTCCGGTCCAAGCCGGCGTTCGTCTGGTCGGAGGACGGGGCTGGTGCTGGCTCTGTCCTCACGTACCGGCAACTTCACGACTCCGTTCAGTTGATATCGTTACGATTGCTGCGCCAGCTGCGGCGGCGCGAAACGGTGGTGATATTATGCTGGGCGGGGCTCGACCTGGTGGAGCTGATCTACGGCTGCCAAAGAGCCGGCCTTGTCAGCGTCCCCATTATGCCGCCCGATCCTCGTTTTACAGCACAGAATAAAAATTATGATCTTACCAGGGTTCTCTCCCAAACTAAACCCAAAGCCGCCATAGCTCACCAAGCTTACATCGCCGCCGTTTTCCGGTATCCATCTAAAGCTATTATTTACTTTTACTTTAAAATAAGATTTTTCTTAAAAAGCACATCTGGTCTACTAAGTAACTATTTTTTTTTCGGAGGACTAAGTAACTATTTAATCGCTAAATTTTAATCAATGCTTATATGTAACAATTTAATAACCACTGTCACCAAAATTTTCATTAAAATTAAGTGTTAATTTCTATCTATAACAATTTAATTTTTTTCCTTTATAAATAAATTTGATTTTTTATTAATTTATCGATCTAAACATATAAGAAATTAAACAATAAAATTTCACAAATAAAAACTAAATCATTATAAATTTTAAACTTCAAAAATTGTTTCTTAAATTACTAATTTGAAAGTATAGCAACTCGTTGATCCAATCAAACTATTGCACCAAAGAGTGTTTTTAACCTAAAAATCTTACATTCTTAACCTTCGTCCTGGCACAAGACTCGAGAAGTTACACTATTCCAACAGACGTTATATTTTTTAAAAAACAGGGAGCTAAATATTATTCTTAAACTATTACTGAACCGATGAAGTTTATATGTATTATTTAACAGGTACCTCTCCTCAACCCAGAGAGACGAGAATCTGGCGACGCTATTACACAACGTAAAATGGATTTCAATGGAGAGTTTAAAGAAACCCAAGGAATTAGAAGAACCCGATTATTCCAAGTTTTGTCACTCTTCATATCGCGGCTGTAAACCGGAGGAACCGTATCTAATCCAGTATACGTCAGGGGCAACAGGACCGCCGAAGGCGGTGGTGGTTTCCGCCGGCGCCGCCGCTCACAACGTCCGGGCGGCGAGGAGAGCCTACGACCTCCACACGGACGCCGTCATCGTGTCGTGGCTCCCACAGTACCACGACTGCGGCCTGATGTTCCTCCTGCTCACGGTCACGTGCGGTGCCACGTGCGTGCTGACCTCACCGGGGCGCTTCGTGGGCAGCCCAATGACGTGGCTGCACCTCGTGACGGCCTTCAGGGCCACGTGCACTCCCGTGCCGTGTTTTGCGCTGCCGCTGGTGGTGAAGCGGGCGAAGGAGAATCCGGGGGTCGCCGGTGGGGTGAATCTGGGGAGCTTGAGGAATTTGATTCTGATAAACGAGCCGATTTACCGGTCGGCGGTGGAGGAGTTTGTGGAGATGTTTGGGTCGGCGGGGTTGGAGCCGGGATGTGTTGCGCCGTCGTACGGGCTGGCGGAGAACTGCACGTTTGTTTCGACAGCGTGGAGAAAGGGGTGGTGGTTTCCGGCGATGCCGAGCCACCGGAAGCTGCTGCCGTGCGGGTGGGTGGGTGGAGAAGATGAAGAGATTGTGGTGGCGGTGGTTAATGGAGAAACTGGGGAGGTGGTTGACGATGACGTTGAAGGAGAGATTTGGGTCTCCTCGCCCAGTAATGCCTCAGGTTTCGAATCCCTAACAAACTCTCAAACTGCGTCGTTTTACTTTTTATATAAGATTATCTATTCCTTATATTTAGATTTAAATATTATTTTTGCCCCTTTAGCTACTCATCTTTCTAAACGTCAACTTTTAAAACAGTGACATTTTTTTCCTCGCTTTATTTTCATTTTTATCATTTATAATTCTAGCACAATATTTTACCAAGTAAAATATTATTAAGATCTTTTAAAATGTAACTTTAAATTTATATAAAATAATTTATCTTGCATATAGAATTTTGCGTTAATTTTACAAATACAAATTACATAGCCATTTTAGAGTGACAAAATAGATGTAGATAAAAAATATAGAAACTAAAATAAAAGAGGACAAAATAATATTTAAAACTTATTTTTATTAGGTTTTCTTTTGTGAACTCATTTATTATTTGGTTTTAAGGAAAACATGGTCTAATATTGAAAAGAAATGTAGTTTTTGAAACTCTAGTTTTCTTTTTGTTTTTAAGTTGCCTTGGTGTGAATTGAGATATAAAAATTGTGAGAAAACAAGGTTTTTTAAAAGAAAATAAGAAAAACATTAAATAAGAAAAAAGATGAATTATCCAATCCAATTTTATCCTCACAATTCATAAATGTTTGATAATTTATTATTTTTAAAAATTTCTATTTTTATATTTAGTTGTGAGGACAGAATAAAATATGATATATGCATGCTATGAGTATGTGTTAGTCTTTTTTTTTTAATTACTATTAATATAAAACAAAAGTTTGACCAAGCCAAAGTGAAAATTTACGAAAGTTTTAGGTGTTAAAATAGAATATTTGAAAATTGAAAGAAAAAATTGTCTAAAGTAATTTATACTTTCTCAATCTCAGGTTACCTTGGGAATCCATCTGTAACCAGAGAGACGTTTCACAGTAAACTGAGGAACAACGAGAGAAGCCGTAGTTTCGTCCGCACCGGCGACAGAGGAGTAATTAAAGGCGCCGACAGATTCCTCTTCGTTATCGGACGGTCCTCCGACATCATTAAACTCAACAGCGACCAACAAATCCACCCTCATTACATCGAATCCATAGCTTACCAAAATTACTCCGCCTTTCTCCGCGGCGGCTGCCTCGCCGCCGTCAAGATTTCGGAGACGGTCGCCGTCGTGGCCGAGATGCAGAGAGCGGACCAAAACGACGCCGAATCGCTGAAGAGACTCTGCGAAGGGATTCGGAGGGCGGCGTTGATCGAAGGAGGAATCGAGGTAGGGCTGGTGGTTTTGGTGAAAAGGGGAAGCGTCCCCAAAACGACGTCGGGGAAGATTAGGAGATCGGCGGCGAAGGAGAAGCTCGCCGGCGGCGGAATGAGCGTGTTAATGGCCGTGAAATTTGGAAAACCTTGCGGCGGTTTGAAGGCATTTAGGGATTTGAAGGATTCGGCGGAGGGAAGAAGCTTCGGAACCTGCCCTCTTTTGCTCTCTCTTCTCTAATTTCTAGTGGGTGAATTTAGAAAATTTTGGCCACACCCACTTTAAATTTTACTGTCATATATATAAAAGATAAAAATGCAGTGTTATTGTGAAATTACTGCTTGTAATTTTAACTGTCACTGCTGAGTATTTTTAATAGCAAAAAAAATTGTTTTTTTCTTCTTCTTAT

mRNA sequence

CTACAATTGGTATGTCAATTTAGCATTAACATTTAAAATAAAGGTTGTAACAACGATTTTAGGGTTTATTAAAATTAAAATCAAAGAACATAGCAATAAAAGAAAACCTTGTTAAAAATTCGGGAGCCAATAATGAATCATAAAGGACAGATTTGTAATTTTGGGCATAATTTGTGTGGGTATAAATAGGGGCAGATGCGGCAAATCAGTTGCAAAAATTAAAGCAAAAAAAAAAAAAAACGAAAGAGAGAGTAGAGTATGCTGTTTGAGAATTTTGATCCTTATTTCCCGGACCAGCCGGTGGTTGACCTGTACCTCCCGGTTTGGGCGAACCTCCCGGCGTTCCGGTCCAAGCCGGCGTTCGTCTGGTCGGAGGACGGGGCTGGTGCTGGCTCTGTCCTCACGTACCGGCAACTTCACGACTCCGTTCAGTTGATATCGTTACGATTGCTGCGCCAGCTGCGGCGGCGCGAAACGGTGGTGATATTATGCTGGGCGGGGCTCGACCTGGTGGAGCTGATCTACGGCTGCCAAAGAGCCGGCCTTGTCAGCGTCCCCATTATGCCGCCCGATCCTCGTTTTACAGCACAGAATAAAAATTATGATCTTACCAGGGTTCTCTCCCAAACTAAACCCAAAGCCGCCATAGCTCACCAAGCTTACATCGCCGCCGTTTTCCGGTACCTCTCCTCAACCCAGAGAGACGAGAATCTGGCGACGCTATTACACAACGTAAAATGGATTTCAATGGAGAGTTTAAAGAAACCCAAGGAATTAGAAGAACCCGATTATTCCAAGTTTTGTCACTCTTCATATCGCGGCTGTAAACCGGAGGAACCGTATCTAATCCAGTATACGTCAGGGGCAACAGGACCGCCGAAGGCGGTGGTGGTTTCCGCCGGCGCCGCCGCTCACAACGTCCGGGCGGCGAGGAGAGCCTACGACCTCCACACGGACGCCGTCATCGTGTCGTGGCTCCCACAGTACCACGACTGCGGCCTGATGTTCCTCCTGCTCACGGTCACGTGCGGTGCCACGTGCGTGCTGACCTCACCGGGGCGCTTCGTGGGCAGCCCAATGACGTGGCTGCACCTCGTGACGGCCTTCAGGGCCACGTGCACTCCCGTGCCGTGTTTTGCGCTGCCGCTGGTGGTGAAGCGGGCGAAGGAGAATCCGGGGGTCGCCGGTGGGGTGAATCTGGGGAGCTTGAGGAATTTGATTCTGATAAACGAGCCGATTTACCGGTCGGCGGTGGAGGAGTTTGTGGAGATGTTTGGGTCGGCGGGGTTGGAGCCGGGATGTGTTGCGCCGTCGTACGGGCTGGCGGAGAACTGCACGTTTGTTTCGACAGCGTGGAGAAAGGGGTGGTGGTTTCCGGCGATGCCGAGCCACCGGAAGCTGCTGCCGTGCGGGTGGGTGGGTGGAGAAGATGAAGAGATTGTGGTGGCGGTGGTTAATGGAGAAACTGGGGAGGTGGTTGACGATGACGTTGAAGGAGAGATTTGGGTCTCCTCGCCCAGTAATGCCTCAGGTTACCTTGGGAATCCATCTGTAACCAGAGAGACGTTTCACAGTAAACTGAGGAACAACGAGAGAAGCCGTAGTTTCGTCCGCACCGGCGACAGAGGAGTAATTAAAGGCGCCGACAGATTCCTCTTCGTTATCGGACGGTCCTCCGACATCATTAAACTCAACAGCGACCAACAAATCCACCCTCATTACATCGAATCCATAGCTTACCAAAATTACTCCGCCTTTCTCCGCGGCGGCTGCCTCGCCGCCGTCAAGATTTCGGAGACGGTCGCCGTCGTGGCCGAGATGCAGAGAGCGGACCAAAACGACGCCGAATCGCTGAAGAGACTCTGCGAAGGGATTCGGAGGGCGGCGTTGATCGAAGGAGGAATCGAGGTAGGGCTGGTGGTTTTGGTGAAAAGGGGAAGCGTCCCCAAAACGACGTCGGGGAAGATTAGGAGATCGGCGGCGAAGGAGAAGCTCGCCGGCGGCGGAATGAGCGTGTTAATGGCCGTGAAATTTGGAAAACCTTGCGGCGGTTTGAAGGCATTTAGGGATTTGAAGGATTCGGCGGAGGGAAGAAGCTTCGGAACCTGCCCTCTTTTGCTCTCTCTTCTCTAATTTCTAGTGGGTGAATTTAGAAAATTTTGGCCACACCCACTTTAAATTTTACTGTCATATATATAAAAGATAAAAATGCAGTGTTATTGTGAAATTACTGCTTGTAATTTTAACTGTCACTGCTGAGTATTTTTAATAGCAAAAAAAATTGTTTTTTTCTTCTTCTTAT

Coding sequence (CDS)

ATGCTGTTTGAGAATTTTGATCCTTATTTCCCGGACCAGCCGGTGGTTGACCTGTACCTCCCGGTTTGGGCGAACCTCCCGGCGTTCCGGTCCAAGCCGGCGTTCGTCTGGTCGGAGGACGGGGCTGGTGCTGGCTCTGTCCTCACGTACCGGCAACTTCACGACTCCGTTCAGTTGATATCGTTACGATTGCTGCGCCAGCTGCGGCGGCGCGAAACGGTGGTGATATTATGCTGGGCGGGGCTCGACCTGGTGGAGCTGATCTACGGCTGCCAAAGAGCCGGCCTTGTCAGCGTCCCCATTATGCCGCCCGATCCTCGTTTTACAGCACAGAATAAAAATTATGATCTTACCAGGGTTCTCTCCCAAACTAAACCCAAAGCCGCCATAGCTCACCAAGCTTACATCGCCGCCGTTTTCCGGTACCTCTCCTCAACCCAGAGAGACGAGAATCTGGCGACGCTATTACACAACGTAAAATGGATTTCAATGGAGAGTTTAAAGAAACCCAAGGAATTAGAAGAACCCGATTATTCCAAGTTTTGTCACTCTTCATATCGCGGCTGTAAACCGGAGGAACCGTATCTAATCCAGTATACGTCAGGGGCAACAGGACCGCCGAAGGCGGTGGTGGTTTCCGCCGGCGCCGCCGCTCACAACGTCCGGGCGGCGAGGAGAGCCTACGACCTCCACACGGACGCCGTCATCGTGTCGTGGCTCCCACAGTACCACGACTGCGGCCTGATGTTCCTCCTGCTCACGGTCACGTGCGGTGCCACGTGCGTGCTGACCTCACCGGGGCGCTTCGTGGGCAGCCCAATGACGTGGCTGCACCTCGTGACGGCCTTCAGGGCCACGTGCACTCCCGTGCCGTGTTTTGCGCTGCCGCTGGTGGTGAAGCGGGCGAAGGAGAATCCGGGGGTCGCCGGTGGGGTGAATCTGGGGAGCTTGAGGAATTTGATTCTGATAAACGAGCCGATTTACCGGTCGGCGGTGGAGGAGTTTGTGGAGATGTTTGGGTCGGCGGGGTTGGAGCCGGGATGTGTTGCGCCGTCGTACGGGCTGGCGGAGAACTGCACGTTTGTTTCGACAGCGTGGAGAAAGGGGTGGTGGTTTCCGGCGATGCCGAGCCACCGGAAGCTGCTGCCGTGCGGGTGGGTGGGTGGAGAAGATGAAGAGATTGTGGTGGCGGTGGTTAATGGAGAAACTGGGGAGGTGGTTGACGATGACGTTGAAGGAGAGATTTGGGTCTCCTCGCCCAGTAATGCCTCAGGTTACCTTGGGAATCCATCTGTAACCAGAGAGACGTTTCACAGTAAACTGAGGAACAACGAGAGAAGCCGTAGTTTCGTCCGCACCGGCGACAGAGGAGTAATTAAAGGCGCCGACAGATTCCTCTTCGTTATCGGACGGTCCTCCGACATCATTAAACTCAACAGCGACCAACAAATCCACCCTCATTACATCGAATCCATAGCTTACCAAAATTACTCCGCCTTTCTCCGCGGCGGCTGCCTCGCCGCCGTCAAGATTTCGGAGACGGTCGCCGTCGTGGCCGAGATGCAGAGAGCGGACCAAAACGACGCCGAATCGCTGAAGAGACTCTGCGAAGGGATTCGGAGGGCGGCGTTGATCGAAGGAGGAATCGAGGTAGGGCTGGTGGTTTTGGTGAAAAGGGGAAGCGTCCCCAAAACGACGTCGGGGAAGATTAGGAGATCGGCGGCGAAGGAGAAGCTCGCCGGCGGCGGAATGAGCGTGTTAATGGCCGTGAAATTTGGAAAACCTTGCGGCGGTTTGAAGGCATTTAGGGATTTGAAGGATTCGGCGGAGGGAAGAAGCTTCGGAACCTGCCCTCTTTTGCTCTCTCTTCTCTAA

Protein sequence

MLFENFDPYFPDQPVVDLYLPVWANLPAFRSKPAFVWSEDGAGAGSVLTYRQLHDSVQLISLRLLRQLRRRETVVILCWAGLDLVELIYGCQRAGLVSVPIMPPDPRFTAQNKNYDLTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKWISMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNVRAARRAYDLHTDAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVTAFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGSAGLEPGCVAPSYGLAENCTFVSTAWRKGWWFPAMPSHRKLLPCGWVGGEDEEIVVAVVNGETGEVVDDDVEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVIKGADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQNYSAFLRGGCLAAVKISETVAVVAEMQRADQNDAESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKLAGGGMSVLMAVKFGKPCGGLKAFRDLKDSAEGRSFGTCPLLLSLL

Homology

[Close]

BLAST of MC08g0677 vs. ExPASy Swiss-Prot
Match: B2HIL4 (4-hydroxyphenylalkanoate adenylyltransferase OS=Mycobacterium marinum (strain ATCC BAA-535 / M) OX=216594 GN=fadD29 PE=1 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 2.1e-40
Identity = 160/574 (27.87%), Postives = 256/574 (44.60%), Query Frame = 0


Query: 43  GAGSVLTYRQLHDSVQLISLRLLRQLRRRETVVILCWAGLDLVELIYGCQRAGLVSVPIM 102
           G    LT+ Q++   ++++  L       + V +L   GL+ +    G   AGL++VP+ 
Sbjct: 74  GFTETLTWWQIYRRAKIVAEELRGYGASGDRVAVLAPQGLEYIIAFLGVLEAGLIAVPL- 133

Query: 103 PPDPRFTAQNKNYDLTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKWI 162
            P P+F   ++   ++  L  + P   +     I  V +Y    +  +    ++  V  +
Sbjct: 134 -PVPQFGIHDER--ISAALQDSTPSVILTTSPVIDEVTKYAPHARAGQGGTPIVVAVDLL 193

Query: 163 SMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNVR 222
            ++S +      E D +   HSS           +QYTSG+T  P  VV+S      N  
Sbjct: 194 DLDSAR------ELDLTPPAHSS--------TAYLQYTSGSTRSPAGVVLSHKNVITNCV 253

Query: 223 AARRAYDLHTDAV---IVSWLPQYHDCGLMF-LLLTVTCGATCVLTSPGRFVGSPMTWLH 282
                Y   T+ V    VSWLP YHD GLM  ++L +    T VL +P  F+  P  W+ 
Sbjct: 254 QLMSDYLGETEKVPSTAVSWLPFYHDMGLMLGIILPMINQDTAVLLNPMAFLQRPARWMQ 313

Query: 283 LVTAFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEM 342
           L+  FR   +  P F   L V+R  +      G++LG +R +    E +  + ++ F++ 
Sbjct: 314 LMGKFRGQISCAPNFGFELAVRRTSDED--MAGLDLGHVRGIGSGAERVNPATLQRFIDR 373

Query: 343 FGSAGLEPGCVAPSYGLAENCTFVSTAWRKGWWFPAMPSHRKLLPCGWV-----GGEDEE 402
           F    L    + PSYGLAE   FV+TA   G    ++    + L  G V       +D  
Sbjct: 374 FAPFNLRDTAIRPSYGLAEATVFVATA-EPGRPPRSVNFDYQSLSVGRVERCANEADDGA 433

Query: 403 IVVAVVNGETGEV--VDDDVE--------GEIWVSSPSNASGYLGNPSVTRETFHSKLRN 462
            +V+  +  T EV  VD +          GEIWV   + A GY  NP +T  TF +KL +
Sbjct: 434 KLVSYGSSWTSEVRIVDPEARTECPAGTVGEIWVQGDNVAMGYWRNPQLTERTFDAKLTD 493

Query: 463 NERSRS---FVRTGDRGVIKGADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQNYSAF 522
                S   ++RTGD GV+   +  LF+ GR  D++ ++     +P  IES   +     
Sbjct: 494 PSPGTSIGPWLRTGDLGVMFEGE--LFITGRIKDLLIVDGSNH-YPDDIESTIQE----- 553

Query: 523 LRGGCLAAVKISE-------TVAVVAEMQRADQNDAESLKRLCEGIRRAALIEGGIEVGL 582
           + GG + A+ + +       T+   A    + Q   + L+ +   I  A      + V  
Sbjct: 554 ITGGRVVAIAVPDADGEKLVTIVEFASWGHSGQEAIDKLRSVKREITSAISRAHRVRVAD 613

Query: 583 VVLVKRGSVPKTTSGKIRRSAAKEKLAGGGMSVL 588
           VVLV  GS+P TTSGK+RRS+  E+    G + L
Sbjct: 614 VVLVATGSIPVTTSGKVRRSSCAERYRNDGFTRL 618

[Close]

BLAST of MC08g0677 vs. ExPASy Swiss-Prot
Match: Q02278 (Long-chain-fatty-acid--AMP ligase FadD28 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=fadD28 PE=3 SV=2)

HSP 1 Score: 167.9 bits (424), Expect = 3.6e-40
Identity = 160/587 (27.26%), Postives = 257/587 (43.78%), Query Frame = 0


Query: 33  PAFV---WSEDGAGAGSVLTYRQLHDSVQLISLRLLRQLRRRETVVILCWAGLDLVELIY 92
           PAF    + +D  G    LT+ QL+     ++  L R     + VVI    GL+ V    
Sbjct: 21  PAFTFMDYEQDWDGVAITLTWSQLYRRTLNVARELSRCGSTGDRVVISAPQGLEYVVAFL 80

Query: 93  GCQRAGLVSVPIMPPDPRFTAQNKNYDLTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRD 152
           G  +AG ++VP+  P    T +  +     VLS + P A +   + +  V ++++    +
Sbjct: 81  GALQAGRIAVPLSVPQGGVTDERSD----SVLSDSSPVAILTTSSAVDDVVQHVARRPGE 140

Query: 153 ENLATLLHNVKWISMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGATGPPKA 212
              +        I ++ L    +L+ P+   F    Y    P   YL QYTSG+T  P  
Sbjct: 141 SPPSI-------IEVDLL----DLDAPNGYTFKEDEY----PSTAYL-QYTSGSTRTPAG 200

Query: 213 VVVSAGAAAHNVRAARRAYDLHTDAV------IVSWLPQYHDCGLMF-LLLTVTCGATCV 272
           VV+S      N       Y   TD +      +VSWLP YHD GL+  +   +  G   V
Sbjct: 201 VVMSHQNVRVNFEQLMSGYFADTDGIPPPNSALVSWLPFYHDMGLVIGICAPILGGYPAV 260

Query: 273 LTSPGRFVGSPMTWLHLVTAFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLIL 332
           LTSP  F+  P  W+HL+ +     +  P FA  L  +R  ++     G +LG++  ++ 
Sbjct: 261 LTSPVSFLQRPARWMHLMASDFHAFSAAPNFAFELAARRTTDDD--MAGRDLGNILTILS 320

Query: 333 INEPIYRSAVEEFVEMFGSAGLEPGCVAPSYGLAENCTFVSTAWRKGWWFPAMP------ 392
            +E +  + ++ F + F    L+   + PSYGLAE   +V+T+       P  P      
Sbjct: 321 GSERVQAATIKRFADRFARFNLQERVIRPSYGLAEATVYVATS------KPGQPPETVDF 380

Query: 393 -------SHRKLLPCGWVGGED-------EEIVVAVVNGETGEVVDDDVEGEIWVSSPSN 452
                   H K  PC   G             +V +V+ +T     D   GEIWV   + 
Sbjct: 381 DTESLSAGHAK--PCAGGGATSLISYMLPRSPIVRIVDSDTCIECPDGTVGEIWVHGDNV 440

Query: 453 ASGYLGNPSVTRETFHSKL---RNNERSRSFVRTGDRGVIKGADRFLFVIGRSSDIIKLN 512
           A+GY   P  +  TF  K+           ++RTGD G +   D  +F+IGR  D++ + 
Sbjct: 441 ANGYWQKPDESERTFGGKIVTPSPGTPEGPWLRTGDSGFV--TDGKMFIIGRIKDLL-IV 500

Query: 513 SDQQIHPHYIESIAYQNYSAFLRGGCLAAVKI-----SETVAVVAEMQRADQNDAESLKR 572
             +   P  IE+   +      RG C AA+ +     +E +  + E+++   +D +++ R
Sbjct: 501 YGRNHSPDDIEATIQE----ITRGRC-AAISVPGDRSTEKLVAIIELKKRGDSDQDAMAR 560

Query: 573 L---CEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEK 579
           L      +  A     G+ V  +VLV  GS+P TTSGK+RR A  E+
Sbjct: 561 LGAIKREVTSALSSSHGLSVADLVLVAPGSIPITTSGKVRRGACVEQ 569

[Close]

BLAST of MC08g0677 vs. ExPASy Swiss-Prot
Match: P9WQ58 (Long-chain-fatty-acid--AMP ligase FadD28 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=fadD28 PE=3 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 3.6e-40
Identity = 160/587 (27.26%), Postives = 257/587 (43.78%), Query Frame = 0


Query: 33  PAFV---WSEDGAGAGSVLTYRQLHDSVQLISLRLLRQLRRRETVVILCWAGLDLVELIY 92
           PAF    + +D  G    LT+ QL+     ++  L R     + VVI    GL+ V    
Sbjct: 21  PAFTFMDYEQDWDGVAITLTWSQLYRRTLNVAQELSRCGSTGDRVVISAPQGLEYVVAFL 80

Query: 93  GCQRAGLVSVPIMPPDPRFTAQNKNYDLTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRD 152
           G  +AG ++VP+  P    T +  +     VLS + P A +   + +  V ++++    +
Sbjct: 81  GALQAGRIAVPLSVPQGGVTDERSD----SVLSDSSPVAILTTSSAVDDVVQHVARRPGE 140

Query: 153 ENLATLLHNVKWISMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGATGPPKA 212
              +        I ++ L    +L+ P+   F    Y    P   YL QYTSG+T  P  
Sbjct: 141 SPPSI-------IEVDLL----DLDAPNGYTFKEDEY----PSTAYL-QYTSGSTRTPAG 200

Query: 213 VVVSAGAAAHNVRAARRAYDLHTDAV------IVSWLPQYHDCGLMF-LLLTVTCGATCV 272
           VV+S      N       Y   TD +      +VSWLP YHD GL+  +   +  G   V
Sbjct: 201 VVMSHQNVRVNFEQLMSGYFADTDGIPPPNSALVSWLPFYHDMGLVIGICAPILGGYPAV 260

Query: 273 LTSPGRFVGSPMTWLHLVTAFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLIL 332
           LTSP  F+  P  W+HL+ +     +  P FA  L  +R  ++     G +LG++  ++ 
Sbjct: 261 LTSPVSFLQRPARWMHLMASDFHAFSAAPNFAFELAARRTTDDD--MAGRDLGNILTILS 320

Query: 333 INEPIYRSAVEEFVEMFGSAGLEPGCVAPSYGLAENCTFVSTAWRKGWWFPAMP------ 392
            +E +  + ++ F + F    L+   + PSYGLAE   +V+T+       P  P      
Sbjct: 321 GSERVQAATIKRFADRFARFNLQERVIRPSYGLAEATVYVATS------KPGQPPETVDF 380

Query: 393 -------SHRKLLPCGWVGGED-------EEIVVAVVNGETGEVVDDDVEGEIWVSSPSN 452
                   H K  PC   G             +V +V+ +T     D   GEIWV   + 
Sbjct: 381 DTESLSAGHAK--PCAGGGATSLISYMLPRSPIVRIVDSDTCIECPDGTVGEIWVHGDNV 440

Query: 453 ASGYLGNPSVTRETFHSKL---RNNERSRSFVRTGDRGVIKGADRFLFVIGRSSDIIKLN 512
           A+GY   P  +  TF  K+           ++RTGD G +   D  +F+IGR  D++ + 
Sbjct: 441 ANGYWQKPDESERTFGGKIVTPSPGTPEGPWLRTGDSGFV--TDGKMFIIGRIKDLL-IV 500

Query: 513 SDQQIHPHYIESIAYQNYSAFLRGGCLAAVKI-----SETVAVVAEMQRADQNDAESLKR 572
             +   P  IE+   +      RG C AA+ +     +E +  + E+++   +D +++ R
Sbjct: 501 YGRNHSPDDIEATIQE----ITRGRC-AAISVPGDRSTEKLVAIIELKKRGDSDQDAMAR 560

Query: 573 L---CEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEK 579
           L      +  A     G+ V  +VLV  GS+P TTSGK+RR A  E+
Sbjct: 561 LGAIKREVTSALSSSHGLSVADLVLVAPGSIPITTSGKVRRGACVEQ 569

[Close]

BLAST of MC08g0677 vs. ExPASy Swiss-Prot
Match: P9WQ59 (Long-chain-fatty-acid--AMP ligase FadD28 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=fadD28 PE=1 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 3.6e-40
Identity = 160/587 (27.26%), Postives = 257/587 (43.78%), Query Frame = 0


Query: 33  PAFV---WSEDGAGAGSVLTYRQLHDSVQLISLRLLRQLRRRETVVILCWAGLDLVELIY 92
           PAF    + +D  G    LT+ QL+     ++  L R     + VVI    GL+ V    
Sbjct: 21  PAFTFMDYEQDWDGVAITLTWSQLYRRTLNVAQELSRCGSTGDRVVISAPQGLEYVVAFL 80

Query: 93  GCQRAGLVSVPIMPPDPRFTAQNKNYDLTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRD 152
           G  +AG ++VP+  P    T +  +     VLS + P A +   + +  V ++++    +
Sbjct: 81  GALQAGRIAVPLSVPQGGVTDERSD----SVLSDSSPVAILTTSSAVDDVVQHVARRPGE 140

Query: 153 ENLATLLHNVKWISMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGATGPPKA 212
              +        I ++ L    +L+ P+   F    Y    P   YL QYTSG+T  P  
Sbjct: 141 SPPSI-------IEVDLL----DLDAPNGYTFKEDEY----PSTAYL-QYTSGSTRTPAG 200

Query: 213 VVVSAGAAAHNVRAARRAYDLHTDAV------IVSWLPQYHDCGLMF-LLLTVTCGATCV 272
           VV+S      N       Y   TD +      +VSWLP YHD GL+  +   +  G   V
Sbjct: 201 VVMSHQNVRVNFEQLMSGYFADTDGIPPPNSALVSWLPFYHDMGLVIGICAPILGGYPAV 260

Query: 273 LTSPGRFVGSPMTWLHLVTAFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLIL 332
           LTSP  F+  P  W+HL+ +     +  P FA  L  +R  ++     G +LG++  ++ 
Sbjct: 261 LTSPVSFLQRPARWMHLMASDFHAFSAAPNFAFELAARRTTDDD--MAGRDLGNILTILS 320

Query: 333 INEPIYRSAVEEFVEMFGSAGLEPGCVAPSYGLAENCTFVSTAWRKGWWFPAMP------ 392
            +E +  + ++ F + F    L+   + PSYGLAE   +V+T+       P  P      
Sbjct: 321 GSERVQAATIKRFADRFARFNLQERVIRPSYGLAEATVYVATS------KPGQPPETVDF 380

Query: 393 -------SHRKLLPCGWVGGED-------EEIVVAVVNGETGEVVDDDVEGEIWVSSPSN 452
                   H K  PC   G             +V +V+ +T     D   GEIWV   + 
Sbjct: 381 DTESLSAGHAK--PCAGGGATSLISYMLPRSPIVRIVDSDTCIECPDGTVGEIWVHGDNV 440

Query: 453 ASGYLGNPSVTRETFHSKL---RNNERSRSFVRTGDRGVIKGADRFLFVIGRSSDIIKLN 512
           A+GY   P  +  TF  K+           ++RTGD G +   D  +F+IGR  D++ + 
Sbjct: 441 ANGYWQKPDESERTFGGKIVTPSPGTPEGPWLRTGDSGFV--TDGKMFIIGRIKDLL-IV 500

Query: 513 SDQQIHPHYIESIAYQNYSAFLRGGCLAAVKI-----SETVAVVAEMQRADQNDAESLKR 572
             +   P  IE+   +      RG C AA+ +     +E +  + E+++   +D +++ R
Sbjct: 501 YGRNHSPDDIEATIQE----ITRGRC-AAISVPGDRSTEKLVAIIELKKRGDSDQDAMAR 560

Query: 573 L---CEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEK 579
           L      +  A     G+ V  +VLV  GS+P TTSGK+RR A  E+
Sbjct: 561 LGAIKREVTSALSSSHGLSVADLVLVAPGSIPITTSGKVRRGACVEQ 569

[Close]

BLAST of MC08g0677 vs. ExPASy Swiss-Prot
Match: Q7TXK5 (4-hydroxyphenylalkanoate adenylyltransferase OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=fadD29 PE=3 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 1.5e-38
Identity = 158/570 (27.72%), Postives = 250/570 (43.86%), Query Frame = 0


Query: 37  WSEDGAGAGSVLTYRQLHDSVQLISLRLLRQLRRRETVVILCWAGLDLVELIYGCQRAGL 96
           +  D AG    +T+ Q+H    +++  L       + V IL   GL+ +    G  +AGL
Sbjct: 65  YDTDPAGFTETVTWWQVHRRAMIVAEELWIYASSGDRVAILAPQGLEYIIAFMGVLQAGL 124

Query: 97  VSVPIMPPDPRFTAQNKNYDLTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLL 156
           ++VP+  P P+F   ++   ++  L  + P   +   + I  V  Y       +  +  +
Sbjct: 125 IAVPL--PVPQFGIHDER--ISSALRDSAPSIILTTSSVIDEVTTYAPHACAAQGQSAPI 184

Query: 157 HNVKWISMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGA 216
             V  +    L   + L+   +           +P   YL QYTSG+T  P  VV+S   
Sbjct: 185 --VVAVDALDLSSSRALDPTRFE----------RPSTAYL-QYTSGSTRAPAGVVLSHKN 244

Query: 217 AAHNVRAARRAYDLHTDAV---IVSWLPQYHDCGLMF-LLLTVTCGATCVLTSPGRFVGS 276
              N       Y   ++ V    VSWLP YHD GLM  ++L +    T VL SP  F+  
Sbjct: 245 VITNCVQLMSDYIGDSEKVPSTPVSWLPFYHDMGLMLGIILPMINQDTAVLMSPMAFLQR 304

Query: 277 PMTWLHLVTAFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAV 336
           P  W+ L+   RA  +  P F   L V+R  ++     G++LG +R ++   E +  + +
Sbjct: 305 PARWMQLLAKHRAQISSAPNFGFELAVRRTSDDD--MAGLDLGHVRTIVTGAERVNVATL 364

Query: 337 EEFVEMFGSAGLEPGCVAPSYGLAENCTFVSTAWRKGWWFPAMPSHRKLLPCGWV----- 396
             F E F    L    + PSYGLAE   +V+TA   G    ++    + L  G       
Sbjct: 365 RRFTERFAPFNLSETAIRPSYGLAEATVYVATA-GPGRAPKSVCFDYQQLSVGQAKRTEN 424

Query: 397 GGEDEEIV---------VAVVNGETGEVVDDDVEGEIWVSSPSNASGYLGNPSVTRETFH 456
           G E   +V         V +V+ ET         GEIWV   +   GY  NP  T  TF 
Sbjct: 425 GSEGANLVSYGAPRASTVRIVDPETRMENPAGTVGEIWVQGDNVGLGYWRNPQQTEATFR 484

Query: 457 SKLRNNERSRS---FVRTGDRGVIKGADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQ 516
           ++L       S   ++RTGD GVI   +  LF+ GR  +++ ++     +P  IE+   +
Sbjct: 485 ARLVTPSPGTSEGPWLRTGDLGVIFEGE--LFITGRIKELLVVDGANH-YPEDIEATIQE 544

Query: 517 NYSAFLRGGCLAAVKI----SETVAVVAEMQRADQNDAESLKRLCEGIRRAALI---EGG 576
                + GG + A+ +    +E +  + E+ +  + D E   RL    R  A        
Sbjct: 545 -----ITGGRVVAIAVPDDRTEKLVTIIELMKRGRTDEEEKNRLRTVKREVASAISRSHR 604

Query: 577 IEVGLVVLVKRGSVPKTTSGKIRRSAAKEK 579
           + V  VV+V  GS+P TTSGK+RRSA+ E+
Sbjct: 605 LRVADVVMVAPGSIPVTTSGKVRRSASVER 606

[Close]

BLAST of MC08g0677 vs. NCBI nr
Match: XP_022144296.1 (uncharacterized protein LOC111014011 [Momordica charantia])

HSP 1 Score: 1053 bits (2724), Expect = 0.0
Identity = 523/523 (100.00%), Postives = 523/523 (100.00%), Query Frame = 0


Query: 102 MPPDPRFTAQNKNYDLTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKW 161
           MPPDPRFTAQNKNYDLTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKW
Sbjct: 1   MPPDPRFTAQNKNYDLTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKW 60

Query: 162 ISMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNV 221
           ISMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNV
Sbjct: 61  ISMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNV 120

Query: 222 RAARRAYDLHTDAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVT 281
           RAARRAYDLHTDAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVT
Sbjct: 121 RAARRAYDLHTDAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVT 180

Query: 282 AFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGS 341
           AFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGS
Sbjct: 181 AFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGS 240

Query: 342 AGLEPGCVAPSYGLAENCTFVSTAWRKGWWFPAMPSHRKLLPCGWVGGEDEEIVVAVVNG 401
           AGLEPGCVAPSYGLAENCTFVSTAWRKGWWFPAMPSHRKLLPCGWVGGEDEEIVVAVVNG
Sbjct: 241 AGLEPGCVAPSYGLAENCTFVSTAWRKGWWFPAMPSHRKLLPCGWVGGEDEEIVVAVVNG 300

Query: 402 ETGEVVDDDVEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVIKG 461
           ETGEVVDDDVEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVIKG
Sbjct: 301 ETGEVVDDDVEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVIKG 360

Query: 462 ADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQNYSAFLRGGCLAAVKISETVAVVAEM 521
           ADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQNYSAFLRGGCLAAVKISETVAVVAEM
Sbjct: 361 ADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQNYSAFLRGGCLAAVKISETVAVVAEM 420

Query: 522 QRADQNDAESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKLAG 581
           QRADQNDAESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKLAG
Sbjct: 421 QRADQNDAESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKLAG 480

Query: 582 GGMSVLMAVKFGKPCGGLKAFRDLKDSAEGRSFGTCPLLLSLL 624
           GGMSVLMAVKFGKPCGGLKAFRDLKDSAEGRSFGTCPLLLSLL
Sbjct: 481 GGMSVLMAVKFGKPCGGLKAFRDLKDSAEGRSFGTCPLLLSLL 523

[Close]

BLAST of MC08g0677 vs. NCBI nr
Match: XP_023546350.1 (uncharacterized protein LOC111805474 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 875 bits (2262), Expect = 3.81e-314
Identity = 460/646 (71.21%), Postives = 521/646 (80.65%), Query Frame = 0


Query: 1   MLFENFDPYFPDQPVVDLYLPVWANLPAFRSKPAFVWSEDGAG----AGSVLTYRQLHDS 60
           M +ENFDP FPDQPVVDLYLPVWA+LPAFRSKPAF+WSEDG       GS LTY QLHDS
Sbjct: 14  MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 73

Query: 61  VQLISLRLLRQLRRRETVVILCWAGLDLVELIYGCQRAGLVSVPIMPPDPRFTAQNKN-Y 120
           VQ IS +L+RQ+RRR+TV++LC  GLDLV+LIYGCQRAGLVSVPI PPDP F  +NKN +
Sbjct: 74  VQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCF--ENKNCH 133

Query: 121 DLTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKWISMESLKKPKELE- 180
            L R LSQTKP+AAIAHQAYIA+VFRYLS +  D  LA LL  V+WISME+L+     E 
Sbjct: 134 HLARALSQTKPRAAIAHQAYIASVFRYLSLSPTDGKLALLLRGVQWISMETLRTCTAAEA 193

Query: 181 --------EPDYSKFCHS--SYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNVRAA 240
                     D   F HS  SY GCKPEEPYLIQYTSGATG PK VVV+AGAAAHNVR A
Sbjct: 194 DQVVSVISSKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRVA 253

Query: 241 RRAYDLHTDAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVTAFR 300
           R+AYDL+ + VIVSWLPQYHDCGLMFLLLTV  GATCVLTSP  FV  P+TWLHL+T F+
Sbjct: 254 RKAYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFK 313

Query: 301 ATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGSAGL 360
           ATCTPVP F LPLV+KR KE     GG++L SLRNLILINEP+YRS VEEFV++F + GL
Sbjct: 314 ATCTPVPSFTLPLVLKRVKEETPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKTVGL 373

Query: 361 EPGCVAPSYGLAENCTFVSTAWRKGW-----WFPAMPSHRKLLPCGWVGGEDE-EIVVAV 420
            PGCV+PSYGLAENCTFVSTAW  G      WFPAMPS+RKLLPC  +  E   EI V V
Sbjct: 374 NPGCVSPSYGLAENCTFVSTAWSGGEGGRRRWFPAMPSYRKLLPCAKLTDESSAEIEVVV 433

Query: 421 VNGETGEVVDDDVEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGV 480
           VNGETGE+VDD VEGEIW+SSPSNASGYLG+PS+TR+TFH KL +N+ S  FVRTGDRGV
Sbjct: 434 VNGETGEIVDDGVEGEIWISSPSNASGYLGHPSLTRDTFHCKL-SNKSSHKFVRTGDRGV 493

Query: 481 IKGADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQNYSAFLRGGCLAAVKISETVAVV 540
           IKG DRFLFVIGR SD+IKLN++Q+IHPHYIESIAY N+SA+LRGGCLAA+KIS+T+AVV
Sbjct: 494 IKGTDRFLFVIGRCSDVIKLNNNQEIHPHYIESIAYNNFSAYLRGGCLAAIKISDTIAVV 553

Query: 541 AEMQRADQNDAESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEK 600
           AEMQR D+NDAESL+++CEGIR+AALIE GIE+GLVVLVKRG+VPKTTSGK++R AAKEK
Sbjct: 554 AEMQRDDKNDAESLRKICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWAAKEK 613

Query: 601 LAGGGMSVLMAVKFGKPCGGLKAFRDLKDSAEGRSFGTCPLLLSLL 624
           LAGGGMSVLMAVKFG  CG  K   DL+        G  PLLLSLL
Sbjct: 614 LAGGGMSVLMAVKFGTTCGVTK---DLE--------GNRPLLLSLL 645

[Close]

BLAST of MC08g0677 vs. NCBI nr
Match: XP_022962563.1 (uncharacterized protein LOC111462961 [Cucurbita moschata])

HSP 1 Score: 868 bits (2242), Expect = 4.18e-311
Identity = 457/645 (70.85%), Postives = 519/645 (80.47%), Query Frame = 0


Query: 1   MLFENFDPYFPDQPVVDLYLPVWANLPAFRSKPAFVWSEDGAG----AGSVLTYRQLHDS 60
           M +ENFDP FPDQPVVDLYLPVWA+LPAFRSKPAF+WSEDG       GS LTY QLHDS
Sbjct: 14  MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 73

Query: 61  VQLISLRLLRQLRRRETVVILCWAGLDLVELIYGCQRAGLVSVPIMPPDPRFTAQNKNYD 120
           VQ IS  L+RQ+RRR+TVVILC  GLDLV+LIYGCQRAGLVSVPI PPDPRF  +N ++ 
Sbjct: 74  VQFISDELIRQVRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPRFENENCHH- 133

Query: 121 LTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKWISMESLKKPKELE-- 180
           L R LSQTKP+AAIAHQAYIA+VFRYLS +  D  LA LL +V+WISME+L+     E  
Sbjct: 134 LARALSQTKPRAAIAHQAYIASVFRYLSLSPTDGKLALLLQSVQWISMETLRTCTAAEAD 193

Query: 181 -------EPDYSKFCHS--SYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNVRAAR 240
                    D   F HS  SY GCKPEEPYLIQYTSGATG PK VVV+AGAAAHNVRAAR
Sbjct: 194 QVVSVISSKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAAR 253

Query: 241 RAYDLHTDAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVTAFRA 300
           +AYDL+ + VIVSWLPQYHDCGLMFLLLTV  GATCVLTSP  FV  P+TWLHL+T F+A
Sbjct: 254 KAYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKA 313

Query: 301 TCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGSAGLE 360
           TCTPVP F LPLV+KR KE     GG++L SLRNLILINEP+YRS VEEFV++F + GL 
Sbjct: 314 TCTPVPSFTLPLVLKRVKEETPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKAVGLN 373

Query: 361 PGCVAPSYGLAENCTFVSTAWRKGW-----WFPAMPSHRKLLPCGWVGGEDE-EIVVAVV 420
           PGCV+PSYGLAENCTFVSTAW  G      WFPAMPS+RKLLP   +  E   EI V VV
Sbjct: 374 PGCVSPSYGLAENCTFVSTAWSGGEGGRRRWFPAMPSYRKLLPSARLMDESSSEIEVVVV 433

Query: 421 NGETGEVVDDDVEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVI 480
           NGETGE+V+D VEGEIW+SSPSNASGYLG+PS+TR+TFH KL +N+ S  FVRTGDRGVI
Sbjct: 434 NGETGEIVEDGVEGEIWISSPSNASGYLGHPSLTRDTFHCKL-SNKSSHKFVRTGDRGVI 493

Query: 481 KGADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQNYSAFLRGGCLAAVKISETVAVVA 540
           KG DRFLFVIGR SD+I L ++Q+IHPHYIESIAY N S++LRGGCLAA+KIS+T+AVVA
Sbjct: 494 KGTDRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNCSSYLRGGCLAAIKISDTIAVVA 553

Query: 541 EMQRADQNDAESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKL 600
           EMQR D+NDAESL+++CEGIR+AALIE GIE+GLVVLVKRG+VPKTTSGK++R AAKEKL
Sbjct: 554 EMQRQDKNDAESLRKICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWAAKEKL 613

Query: 601 AGGGMSVLMAVKFGKPCGGLKAFRDLKDSAEGRSFGTCPLLLSLL 624
           AGGGMSVLMAVKFGK CG  K   DL+ +         PLLLSLL
Sbjct: 614 AGGGMSVLMAVKFGKTCGVTK---DLETNR--------PLLLSLL 645

[Close]

BLAST of MC08g0677 vs. NCBI nr
Match: KAG7029389.1 (fadD26, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 863 bits (2229), Expect = 2.46e-309
Identity = 456/645 (70.70%), Postives = 514/645 (79.69%), Query Frame = 0


Query: 1   MLFENFDPYFPDQPVVDLYLPVWANLPAFRSKPAFVWSEDGAG----AGSVLTYRQLHDS 60
           M +ENFDP FPDQPVVDLYLPVWA+LPAFRSKPAF+WSEDG       GS LTY QLHDS
Sbjct: 1   MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 60

Query: 61  VQLISLRLLRQLRRRETVVILCWAGLDLVELIYGCQRAGLVSVPIMPPDPRFTAQNKNYD 120
           VQ IS  L+RQ+RRR+TVVILC  GLDLV+LIYGCQRAGLVSVPI PPDPRF  +N ++ 
Sbjct: 61  VQFISDELIRQVRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPRFENENCHH- 120

Query: 121 LTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKWISMESLKKPKELE-- 180
           L R LSQTKP AAIAHQAYIA+VFRYLS +  D  LA LL +V+WISMESL+     E  
Sbjct: 121 LARALSQTKPWAAIAHQAYIASVFRYLSLSPIDGKLALLLRSVQWISMESLRTCTAAEAD 180

Query: 181 -------EPDYSKFCHS--SYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNVRAAR 240
                    D   F HS  SY GCKPEEPYLIQYTSGATG PK VVV+AGAAAHNVR AR
Sbjct: 181 QVVSVISRKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRVAR 240

Query: 241 RAYDLHTDAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVTAFRA 300
           +AYDL+ + VIVSWLPQYHDCGLMFLLLTV  GATCVLTSP  FV  P+TWLHL+T F+A
Sbjct: 241 KAYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKA 300

Query: 301 TCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGSAGLE 360
           TCTPVP F LPLV+KR KE     GG++L SLRNLILINEP+YRS VEEFV++F + GL 
Sbjct: 301 TCTPVPSFTLPLVLKRVKEETPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKAVGLN 360

Query: 361 PGCVAPSYGLAENCTFVSTAWRKGW-----WFPAMPSHRKLLPCGWVGGEDE-EIVVAVV 420
           PGCV+PSYGLAENCTFVSTAW  G      WFPAMPS+RKLLP   +  E   EI V VV
Sbjct: 361 PGCVSPSYGLAENCTFVSTAWSGGEGGRRRWFPAMPSYRKLLPSARLMDESSSEIEVVVV 420

Query: 421 NGETGEVVDDDVEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVI 480
           NGETGE+V+D VEGEIW+SSPSNASGYLG+PS+TR+TFH KL +N+ S  FVRTGDRGVI
Sbjct: 421 NGETGEIVEDGVEGEIWISSPSNASGYLGHPSLTRDTFHCKL-SNKSSHKFVRTGDRGVI 480

Query: 481 KGADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQNYSAFLRGGCLAAVKISETVAVVA 540
           KG DRFLFVIGR SD+I L ++Q+IHPHYIESIAY N S++LRGGCLAA+KIS+T+AVVA
Sbjct: 481 KGTDRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNCSSYLRGGCLAAIKISDTIAVVA 540

Query: 541 EMQRADQNDAESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKL 600
           EMQR D+NDAESL+++CEGIR+AALIE GIE+GLVVLVKRG+VPKTTSGK++R AAKEKL
Sbjct: 541 EMQRQDKNDAESLRKICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWAAKEKL 600

Query: 601 AGGGMSVLMAVKFGKPCGGLKAFRDLKDSAEGRSFGTCPLLLSLL 624
           AGGGMSVLMAVKFGK CG        KD    R     PLLLS L
Sbjct: 601 AGGGMSVLMAVKFGKTCG------ITKDLETNR-----PLLLSFL 632

[Close]

BLAST of MC08g0677 vs. NCBI nr
Match: KAG6598448.1 (hypothetical protein SDJN03_08226, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 859 bits (2219), Expect = 7.85e-308
Identity = 456/645 (70.70%), Postives = 516/645 (80.00%), Query Frame = 0


Query: 1   MLFENFDPYFPDQPVVDLYLPVWANLPAFRSKPAFVWSEDGAG----AGSVLTYRQLHDS 60
           M +ENFDP FPDQPVVDLYLPVWA+LPAFRSKPAF+WSEDG       GS LTY QLHDS
Sbjct: 1   MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 60

Query: 61  VQLISLRLLRQLRRRETVVILCWAGLDLVELIYGCQRAGLVSVPIMPPDPRFTAQNKNYD 120
           VQ IS  L+RQ+RRR+TVVILC  GLDLV+LIYGCQRAGLVSVPI PPDPRF  +N ++ 
Sbjct: 61  VQFISDELIRQVRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPRFENENCHH- 120

Query: 121 LTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKWISMESLKKPKELE-- 180
           L R LSQTKP AAIAHQAYIA+VFRYLS +  D  LA LL +V+WISMESL+     E  
Sbjct: 121 LARALSQTKPWAAIAHQAYIASVFRYLSLSLIDGKLALLLRSVQWISMESLRTCTAAEAD 180

Query: 181 -------EPDYSKFCHS--SYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNVRAAR 240
                    D   F HS  SY GCKPEEPYLIQYTSGATG PK VVV+AGAAAHNVR AR
Sbjct: 181 QVVSVISRKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRVAR 240

Query: 241 RAYDLHTDAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVTAFRA 300
           +AYDL+ + VIVSWLPQYHDCGLMFLLLTV  GATCVLTSP  FV  P+TWLHL+T F+A
Sbjct: 241 KAYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKA 300

Query: 301 TCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGSAGLE 360
           TCTPVP F LPLV+KR KE     GG++L SLRNLILINEP+YRS VEEFV++F + GL 
Sbjct: 301 TCTPVPSFTLPLVLKRVKEETPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKAVGLN 360

Query: 361 PGCVAPSYGLAENCTFVSTAWRKGW-----WFPAMPSHRKLLPCGWVGGEDE-EIVVAVV 420
           PGCV+PSYGLAENCTFVSTAW  G      WFPAMPS+RKLLP   +  E   EI V VV
Sbjct: 361 PGCVSPSYGLAENCTFVSTAWSGGEGGRRRWFPAMPSYRKLLPSARLMDESSSEIEVVVV 420

Query: 421 NGETGEVVDDDVEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVI 480
           NGETGE+V+D VEGEIW+SSPSNASGYLG+PS+TR+TFH KL +N+ S  FVRTGDRGVI
Sbjct: 421 NGETGEIVEDGVEGEIWISSPSNASGYLGHPSLTRDTFHCKL-SNKSSHKFVRTGDRGVI 480

Query: 481 KGADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQNYSAFLRGGCLAAVKISETVAVVA 540
           KG DRFLFVIGR SD+I L ++Q+IHPHYIESIAY N S++LRGGCLAA+KIS+T+AVVA
Sbjct: 481 KGTDRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNCSSYLRGGCLAAIKISDTIAVVA 540

Query: 541 EMQRADQNDAESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKL 600
           EMQR D+NDAESL+++CEGIR+AALIEG IE+GLVVLVKRG+VPKTTSGK++R AAKEKL
Sbjct: 541 EMQRQDKNDAESLRKICEGIRKAALIEG-IELGLVVLVKRGNVPKTTSGKVKRWAAKEKL 600

Query: 601 AGGGMSVLMAVKFGKPCGGLKAFRDLKDSAEGRSFGTCPLLLSLL 624
           AGGGMSVLMAVKFGK CG  K   DL+ +         PLLLS L
Sbjct: 601 AGGGMSVLMAVKFGKTCGVTK---DLETNR--------PLLLSFL 631

[Close]

BLAST of MC08g0677 vs. ExPASy TrEMBL
Match: A0A6J1CSZ4 (uncharacterized protein LOC111014011 OS=Momordica charantia OX=3673 GN=LOC111014011 PE=4 SV=1)

HSP 1 Score: 1053 bits (2724), Expect = 0.0
Identity = 523/523 (100.00%), Postives = 523/523 (100.00%), Query Frame = 0


Query: 102 MPPDPRFTAQNKNYDLTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKW 161
           MPPDPRFTAQNKNYDLTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKW
Sbjct: 1   MPPDPRFTAQNKNYDLTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKW 60

Query: 162 ISMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNV 221
           ISMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNV
Sbjct: 61  ISMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNV 120

Query: 222 RAARRAYDLHTDAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVT 281
           RAARRAYDLHTDAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVT
Sbjct: 121 RAARRAYDLHTDAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVT 180

Query: 282 AFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGS 341
           AFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGS
Sbjct: 181 AFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGS 240

Query: 342 AGLEPGCVAPSYGLAENCTFVSTAWRKGWWFPAMPSHRKLLPCGWVGGEDEEIVVAVVNG 401
           AGLEPGCVAPSYGLAENCTFVSTAWRKGWWFPAMPSHRKLLPCGWVGGEDEEIVVAVVNG
Sbjct: 241 AGLEPGCVAPSYGLAENCTFVSTAWRKGWWFPAMPSHRKLLPCGWVGGEDEEIVVAVVNG 300

Query: 402 ETGEVVDDDVEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVIKG 461
           ETGEVVDDDVEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVIKG
Sbjct: 301 ETGEVVDDDVEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVIKG 360

Query: 462 ADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQNYSAFLRGGCLAAVKISETVAVVAEM 521
           ADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQNYSAFLRGGCLAAVKISETVAVVAEM
Sbjct: 361 ADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQNYSAFLRGGCLAAVKISETVAVVAEM 420

Query: 522 QRADQNDAESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKLAG 581
           QRADQNDAESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKLAG
Sbjct: 421 QRADQNDAESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKLAG 480

Query: 582 GGMSVLMAVKFGKPCGGLKAFRDLKDSAEGRSFGTCPLLLSLL 624
           GGMSVLMAVKFGKPCGGLKAFRDLKDSAEGRSFGTCPLLLSLL
Sbjct: 481 GGMSVLMAVKFGKPCGGLKAFRDLKDSAEGRSFGTCPLLLSLL 523

[Close]

BLAST of MC08g0677 vs. ExPASy TrEMBL
Match: A0A6J1HF51 (uncharacterized protein LOC111462961 OS=Cucurbita moschata OX=3662 GN=LOC111462961 PE=4 SV=1)

HSP 1 Score: 868 bits (2242), Expect = 2.02e-311
Identity = 457/645 (70.85%), Postives = 519/645 (80.47%), Query Frame = 0


Query: 1   MLFENFDPYFPDQPVVDLYLPVWANLPAFRSKPAFVWSEDGAG----AGSVLTYRQLHDS 60
           M +ENFDP FPDQPVVDLYLPVWA+LPAFRSKPAF+WSEDG       GS LTY QLHDS
Sbjct: 14  MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 73

Query: 61  VQLISLRLLRQLRRRETVVILCWAGLDLVELIYGCQRAGLVSVPIMPPDPRFTAQNKNYD 120
           VQ IS  L+RQ+RRR+TVVILC  GLDLV+LIYGCQRAGLVSVPI PPDPRF  +N ++ 
Sbjct: 74  VQFISDELIRQVRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPRFENENCHH- 133

Query: 121 LTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKWISMESLKKPKELE-- 180
           L R LSQTKP+AAIAHQAYIA+VFRYLS +  D  LA LL +V+WISME+L+     E  
Sbjct: 134 LARALSQTKPRAAIAHQAYIASVFRYLSLSPTDGKLALLLQSVQWISMETLRTCTAAEAD 193

Query: 181 -------EPDYSKFCHS--SYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNVRAAR 240
                    D   F HS  SY GCKPEEPYLIQYTSGATG PK VVV+AGAAAHNVRAAR
Sbjct: 194 QVVSVISSKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAAR 253

Query: 241 RAYDLHTDAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVTAFRA 300
           +AYDL+ + VIVSWLPQYHDCGLMFLLLTV  GATCVLTSP  FV  P+TWLHL+T F+A
Sbjct: 254 KAYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKA 313

Query: 301 TCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGSAGLE 360
           TCTPVP F LPLV+KR KE     GG++L SLRNLILINEP+YRS VEEFV++F + GL 
Sbjct: 314 TCTPVPSFTLPLVLKRVKEETPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKAVGLN 373

Query: 361 PGCVAPSYGLAENCTFVSTAWRKGW-----WFPAMPSHRKLLPCGWVGGEDE-EIVVAVV 420
           PGCV+PSYGLAENCTFVSTAW  G      WFPAMPS+RKLLP   +  E   EI V VV
Sbjct: 374 PGCVSPSYGLAENCTFVSTAWSGGEGGRRRWFPAMPSYRKLLPSARLMDESSSEIEVVVV 433

Query: 421 NGETGEVVDDDVEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVI 480
           NGETGE+V+D VEGEIW+SSPSNASGYLG+PS+TR+TFH KL +N+ S  FVRTGDRGVI
Sbjct: 434 NGETGEIVEDGVEGEIWISSPSNASGYLGHPSLTRDTFHCKL-SNKSSHKFVRTGDRGVI 493

Query: 481 KGADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQNYSAFLRGGCLAAVKISETVAVVA 540
           KG DRFLFVIGR SD+I L ++Q+IHPHYIESIAY N S++LRGGCLAA+KIS+T+AVVA
Sbjct: 494 KGTDRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNCSSYLRGGCLAAIKISDTIAVVA 553

Query: 541 EMQRADQNDAESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKL 600
           EMQR D+NDAESL+++CEGIR+AALIE GIE+GLVVLVKRG+VPKTTSGK++R AAKEKL
Sbjct: 554 EMQRQDKNDAESLRKICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWAAKEKL 613

Query: 601 AGGGMSVLMAVKFGKPCGGLKAFRDLKDSAEGRSFGTCPLLLSLL 624
           AGGGMSVLMAVKFGK CG  K   DL+ +         PLLLSLL
Sbjct: 614 AGGGMSVLMAVKFGKTCGVTK---DLETNR--------PLLLSLL 645

[Close]

BLAST of MC08g0677 vs. ExPASy TrEMBL
Match: A0A6J1K7R6 (uncharacterized protein LOC111492429 OS=Cucurbita maxima OX=3661 GN=LOC111492429 PE=4 SV=1)

HSP 1 Score: 848 bits (2192), Expect = 5.02e-304
Identity = 447/645 (69.30%), Postives = 512/645 (79.38%), Query Frame = 0


Query: 1   MLFENFDPYFPDQPVVDLYLPVWANLPAFRSKPAFVWSEDGAG----AGSVLTYRQLHDS 60
           M +ENFDP FPDQPVVDLYLPVWA+LPAFRSKPAF+WSEDG       GS LTY QLHDS
Sbjct: 1   MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 60

Query: 61  VQLISLRLLRQLRRRETVVILCWAGLDLVELIYGCQRAGLVSVPIMPPDPRFTAQNKNYD 120
           VQ IS +L+RQ+RRR+TV++LC  GLDLV+LIYGCQRAGLVSVPI PPDP F  +N ++ 
Sbjct: 61  VQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFENENCHH- 120

Query: 121 LTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKWISMESLKKPKELE-- 180
             R LSQTKP+A IAH+ YI +VFRYLSS+Q D  LA LL +V+WISME+L+     E  
Sbjct: 121 FARALSQTKPRAVIAHEPYIVSVFRYLSSSQIDGKLALLLQSVQWISMETLRTCTAAEAD 180

Query: 181 -------EPDYSKFCHS--SYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNVRAAR 240
                    D   F HS  SY GCKPEEPYLIQYTSGATG PK VVV+AGAAAHNVRAAR
Sbjct: 181 QVVSVISSKDRPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAAR 240

Query: 241 RAYDLHTDAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVTAFRA 300
           +AYDL+ + VIVSWLPQYHDCGLMFLLLTV  GATCVLTSP  FV  P+TWLHL+T F+A
Sbjct: 241 KAYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKA 300

Query: 301 TCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGSAGLE 360
           TCTPVP F LPLV+KR KE     G ++L  LRNLILINEP+YRS VEEFV++F + GL 
Sbjct: 301 TCTPVPSFTLPLVLKRVKEETPPGGDLDLCCLRNLILINEPVYRSGVEEFVDVFKAVGLN 360

Query: 361 PGCVAPSYGLAENCTFVSTAWRKGWW-----FPAMPSHRKLLPCGWV-GGEDEEIVVAVV 420
           PGCV+PSYGLAEN TFVSTAW  G       FPAMPS+RKLLPC  +  G   EI V VV
Sbjct: 361 PGCVSPSYGLAENSTFVSTAWSGGEGGLRRCFPAMPSYRKLLPCARLTDGSSAEIEVVVV 420

Query: 421 NGETGEVVDDDVEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVI 480
           NGETGE+VDD VEGEIW+SSPSNASGYLG+P +TR+TFH KL +N+ S  FVRTGDRGVI
Sbjct: 421 NGETGEMVDDGVEGEIWISSPSNASGYLGHPLLTRDTFHCKL-SNKSSHKFVRTGDRGVI 480

Query: 481 KGADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQNYSAFLRGGCLAAVKISETVAVVA 540
           KGADRFLFVIGR SD+I L ++Q+IHPHYIESIAY N+SA+LRGGCLAA+K+S+T+AVVA
Sbjct: 481 KGADRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNFSAYLRGGCLAAIKVSDTIAVVA 540

Query: 541 EMQRADQNDAESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKL 600
           EMQR D+NDAE+L+++CEGIR+AALIE GIE+GLVVLVKRG+VPKTTSGK++R  AKEKL
Sbjct: 541 EMQREDKNDAETLRQICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWLAKEKL 600

Query: 601 AGGGMSVLMAVKFGKPCGGLKAFRDLKDSAEGRSFGTCPLLLSLL 624
           AGGGMSVLMAVKFG  CG  K   DL         G  PLLLSLL
Sbjct: 601 AGGGMSVLMAVKFGTICGVTK---DLD--------GNRPLLLSLL 632

[Close]

BLAST of MC08g0677 vs. ExPASy TrEMBL
Match: A0A0A0LL23 (AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G361690 PE=4 SV=1)

HSP 1 Score: 833 bits (2152), Expect = 4.34e-298
Identity = 439/635 (69.13%), Postives = 513/635 (80.79%), Query Frame = 0


Query: 1   MLFENFDPYFPDQPVVDLYLPVWANLPAFRSKPAFVWSEDGAGAG----SVLTYRQLHDS 60
           ML+ENFDP FP+QPVVD YLPVWA+LPAF+SKPAF+WSEDG        S LTYRQLHDS
Sbjct: 1   MLYENFDPLFPEQPVVDRYLPVWASLPAFQSKPAFIWSEDGTAKAINEDSFLTYRQLHDS 60

Query: 61  VQLISLRLLRQLRRRETVVILCWAGLDLVELIYGCQRAGLVSVPIMPPDPRFTAQNKNYD 120
           VQLI+ +LLRQLRRR+TVV+LC AGLD V+LIYGCQRAGLVSVPI PPD  F+     + 
Sbjct: 61  VQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDV-FSENENCHH 120

Query: 121 LTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKWISMESLKKPKELEEP 180
           L R LSQTKP+AAIAHQ+YI  VFRY+SS+  D+ LA LL  V+WISMESLK+P +  E 
Sbjct: 121 LARALSQTKPRAAIAHQSYINTVFRYISSSA-DKKLALLLKGVRWISMESLKQPHKESEL 180

Query: 181 DYSK----FCHSS-YRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNVRAARRAYDLH 240
           +  K    F HSS Y GC P++PYLIQYTSGAT   KAVV++AGAAAHNVRAAR+AYDL+
Sbjct: 181 NQHKHQSSFYHSSSYSGCNPDDPYLIQYTSGATAISKAVVITAGAAAHNVRAARKAYDLN 240

Query: 241 TDAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVTAFRATCTPVP 300
            + +IVSWLPQYHDCGLMFLLLTV  GATCVLTSP  FV  P+TWLHL+TAF+ATCTPVP
Sbjct: 241 PNDLIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLHLITAFKATCTPVP 300

Query: 301 CFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGSAGLEPGCVAP 360
            F LPLV+KR   + G AG ++L SLRNLILINEPIYRS VEEFVE+F + GL+PGCV+P
Sbjct: 301 SFTLPLVLKRVGYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVEVFKTVGLDPGCVSP 360

Query: 361 SYGLAENCTFVSTAWRKGWWFPAMPSHRKLLPCGWV-GGEDEEIVVAVVNGETGEVVDDD 420
           SYGLAENCTFVSTAW  G  FPAMPS+R+LLP G +  G  +EI V VVN ETGEVV+D 
Sbjct: 361 SYGLAENCTFVSTAWCGGGGFPAMPSYRQLLPSGRLRDGMCKEIEVVVVNEETGEVVEDG 420

Query: 421 VEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVIKGADRFLFVIG 480
           VEGEIWVSSPSNA GYLG+PSVT ETFHSKL+N   S +FVRTGDRGVIKG+DRFLFVIG
Sbjct: 421 VEGEIWVSSPSNALGYLGHPSVTEETFHSKLKNKS-SLNFVRTGDRGVIKGSDRFLFVIG 480

Query: 481 RSSDIIKLNSD-QQIHPHYIESIAYQNYSAFLRGGCLAAVKISETVAVVAEMQRADQNDA 540
           R SD+IKLN++ QQIHPHYIES +Y N+SA+LRGGC+AAVK+S T+A+VAEMQR D++DA
Sbjct: 481 RCSDVIKLNNNNQQIHPHYIESTSYNNFSAYLRGGCIAAVKVSGTIALVAEMQRDDRHDA 540

Query: 541 ESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKLAGGGMSVLMA 600
           E L+++CE IR+A LIE GIE+GLVVLVKRG+V KTTSGK++R   KEKLAGGG+ VLMA
Sbjct: 541 ELLRKICEDIRKAVLIEEGIELGLVVLVKRGNVSKTTSGKVKRWVVKEKLAGGGLGVLMA 600

Query: 601 VKFGKPCGGLKAFRDLKDSAEGRSFGTCPLLLSLL 624
            +FGK C       DLK   E   F T P+L+S+L
Sbjct: 601 FEFGKNC------VDLKRKGE---FETRPVLISML 623

[Close]

BLAST of MC08g0677 vs. ExPASy TrEMBL
Match: A5AZE1 (AMP-binding domain-containing protein OS=Vitis vinifera OX=29760 GN=VITISV_041652 PE=4 SV=1)

HSP 1 Score: 701 bits (1810), Expect = 4.92e-246
Identity = 369/598 (61.71%), Postives = 447/598 (74.75%), Query Frame = 0


Query: 1   MLFENFDPYFPDQPVVDLYLPVWANLPAFRSKPAFVWSEDGAGAGSVLTYRQLHDSVQLI 60
           M +EN+DPYFPDQPVVDLYLPVWANLPAFRSK AF+W+EDGA     LTY QL+ S Q I
Sbjct: 1   MNYENYDPYFPDQPVVDLYLPVWANLPAFRSKAAFIWAEDGA----TLTYAQLNSSAQSI 60

Query: 61  SLRLLRQLRRRETVVILCWAGLDLVELIYGCQRAGLVSVPIMPPDPRFTAQNKNYDLTRV 120
           S ++L +L+R +TVVILC  GL+LVE+I+GCQRAGL+SVPI PP P FT ++ +Y L RV
Sbjct: 61  SAKILHRLKRGDTVVILCSPGLELVEIIFGCQRAGLLSVPIFPPHPSFTKED-HYHLVRV 120

Query: 121 LSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKWISMESLKKPKELEEPDYSK 180
           LSQTKPKAAIA   +IAAV RY+SS   D+ L  LL  V+WIS + +K  KE      S 
Sbjct: 121 LSQTKPKAAIAQHGFIAAVKRYVSSPVSDKRLCELLQGVRWISTDDVKDGKEGNSSSGSL 180

Query: 181 FCHSSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNVRAARRAYDLHTDAVIVSWL 240
                Y GCK +E YL+Q+TSGATG PK V+V+AGAAAHNVR AR+AY LH ++VIVSWL
Sbjct: 181 ----PYHGCKADEMYLVQFTSGATGIPKPVLVTAGAAAHNVRTARKAYHLHPNSVIVSWL 240

Query: 241 PQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVTAFRATCTPVPCFALPLVVK 300
           PQYHDCGLMFLLLT+  GATCVLTSP  FV  P  WL L++ ++ATCTPVP FALPLVVK
Sbjct: 241 PQYHDCGLMFLLLTIVSGATCVLTSPIAFVNRPRLWLELISEYKATCTPVPSFALPLVVK 300

Query: 301 RAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGSAGLEPGCVAPSYGLAENCT 360
           R   + G    +NL SL+NLI+INEPIYR +VEEFV  F   GL P C++PSYGLAENCT
Sbjct: 301 RGGIDKGTLP-INLWSLKNLIIINEPIYRDSVEEFVNAFLPFGLNPACISPSYGLAENCT 360

Query: 361 FVSTAWRKGWW---FPAMPSHRKLLPCGWVGGE---DEEIVVAVVNGETGEVVDDDVEGE 420
           FVSTAWR       FP +PS+ KLLP   +  E   +EE+ + VVN ET E V+D +EGE
Sbjct: 361 FVSTAWRNNGGSSSFPNIPSYNKLLPSARLASECIDEEEMDILVVNEETQEPVEDGIEGE 420

Query: 421 IWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVIKGADRFLFVIGRSSD 480
           IWVSS SNASGYLG+PS+TR  F  +L +N+ S  FVRTGDRG++KG +R+LF+ GRSSD
Sbjct: 421 IWVSSSSNASGYLGHPSITRYVFLGRL-SNKVSSCFVRTGDRGIVKGEERYLFITGRSSD 480

Query: 481 IIKLNSDQQIHPHYIESIAYQNYSAFLRGGCLAAVKISETVAVVAEMQRADQNDAESLKR 540
           II+L S +QIHPHYIE+ AY +   FLRGGCLAA KIS TV ++AEMQR ++ +   LK 
Sbjct: 481 IIELQSGEQIHPHYIETAAYNSCQKFLRGGCLAAFKISRTVVLIAEMQRREE-ETRILKI 540

Query: 541 LCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKLAGGGMSVLMAVKF 592
           +CEGIR + L E  I +GLVVLVK G VPKTTSGKI+R A K+KL GG M V+M ++F
Sbjct: 541 ICEGIRDSVLREEKIGIGLVVLVKAGCVPKTTSGKIQRWATKDKLIGGKMKVVMEMQF 586

[Close]

BLAST of MC08g0677 vs. TAIR 10
Match: AT1G20500.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 75.5 bits (184), Expect = 1.7e-13
Identity = 112/471 (23.78%), Postives = 190/471 (40.34%), Query Frame = 0


Query: 30  RSKPAFVWSEDGAGAGSVLTYRQLHDSVQLISLRLLRQ--LRRRETVVILCWAGLDLVEL 89
           R K AF+     A  G  LT+  L  +V  ++  L  +  +RR + V+IL    + +  +
Sbjct: 50  RGKTAFI----DAATGQCLTFSDLWRAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVV 109

Query: 90  IYGCQRAGLV--SVPIMPPDPRFTAQ--NKNYDLTRVLSQTKPKAAIAHQAYIAAVFRYL 149
                  G V  +   +      + Q  + N  L     Q  PK  +A    I+ V   L
Sbjct: 110 CLSVMSLGAVFTTANTLNTSGEISKQIADSNPTLVFTTRQLAPKLPVA----ISVV---L 169

Query: 150 SSTQRDENLATLLHNVKWISMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGA 209
           +  +  + L + +  V  +S    K+P      D              ++  ++ Y+SG 
Sbjct: 170 TDDEVYQELTSAIRVVGILSEMVKKEPSGQRVRD----------RVNQDDTAMMLYSSGT 229

Query: 210 TGPPKAVVVS-AGAAAHNVRAARRAYDLHTDAVIVSWLPQYHDCGLM-FLLLTVTCGATC 269
           TGP K V+ S     AH  R    + +L  D + +  +P +H  GL+ F + TV  G+T 
Sbjct: 230 TGPSKGVISSHRNLTAHVARFI--SDNLKRDDIFICTVPMFHTYGLLTFAMGTVALGSTV 289

Query: 270 VLTSPGRFVGSPMTWLHLVTAFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLI 329
           V+    RF    M  +  V   RAT       A P++V    +   +    +L SL+ + 
Sbjct: 290 VILR--RFQLHDM--MDAVEKHRATAL---ALAPPVLVAMINDADLIKAKYDLSSLKTVR 349

Query: 330 LINEPIYRSAVEEFVEMFGSAGLEPGCVAPSYGLAENCTFVSTAWRKGWWFPAMPSHRKL 389
               P+ +   E F+E + +  +  G     Y L E+          G  F       + 
Sbjct: 350 CGGAPLSKEVTEGFLEKYPTVDILQG-----YALTES--------NGGGAFTNSAEESRR 409

Query: 390 LPCGWVGGEDEEIVVAVVNGETGEVVDDDVEGEIWVSSPSNASGYLGNPSVTRETFHSKL 449
              G  G    ++   +V+  TG  +  +  GE+W+  PS + GY  N   T ET + + 
Sbjct: 410 Y--GTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQEATNETINLE- 465

Query: 450 RNNERSRSFVRTGDRGVIKGADRFLFVIGRSSDIIKLNSDQQIHPHYIESI 493
                   +++TGD   I   D FLFV+ R  ++IK     Q+ P  +E++
Sbjct: 470 -------GWLKTGDLCYI-DEDGFLFVVDRLKELIKYKG-YQVPPAELEAL 465

[Close]

BLAST of MC08g0677 vs. TAIR 10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 66.6 bits (161), Expect = 7.9e-11
Identity = 91/381 (23.88%), Postives = 146/381 (38.32%), Query Frame = 0


Query: 199 YTSGATGPPKAVVVSAGAAAHNVRAARRAYDLHTDAVIVSWLPQYHDCGL-MFLLLTVTC 258
           Y+SG TG  K V++S       V+A R  + L    +    +P  H  G   F    +  
Sbjct: 220 YSSGTTGTSKGVMLSHRNLIALVQAYRARFGLEQRTICT--IPMCHIFGFGGFATGLIAL 279

Query: 259 GATCVLTSPGRFVGSPMTWLHLVTAFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSL 318
           G T V       V        L++A     +       P+VV        +    +L SL
Sbjct: 280 GWTIV-------VLPKFDMAKLLSAVETHRSSYLSLVPPIVVAMVNGANEINSKYDLSSL 339

Query: 319 RNLILINEPIYRSAVEEFVEMFGSAGLEPGCVAPSYGLAENCTFVSTAWRKGWWFPAMPS 378
             ++    P+ R   E+FVE +    +  G     YGL E+    ++ + K         
Sbjct: 340 HTVVAGGAPLSREVTEKFVENYPKVKILQG-----YGLTESTAIAASMFNK--------- 399

Query: 379 HRKLLPCGWVGGEDEEIVVAVVNGETGEVVDDDVEGEIWVSSPSNASGYLGNPSVTRETF 438
             +    G  G     +   +V+ +TG V+  +  GE+W+ SP+   GY  N   T  T 
Sbjct: 400 -EETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATASTI 459

Query: 439 HSKLRNNERSRSFVRTGDRGVIKGADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQNY 498
            S+         +++TGD   I G D F+FV+ R  ++IK N              YQ  
Sbjct: 460 DSE--------GWLKTGDLCYIDG-DGFVFVVDRLKELIKCN-------------GYQVA 519

Query: 499 SAFLRGGCLAAVKISETVAVVAEMQRADQNDAESLKRLCEGIRRAALIEGGI--EVGLVV 558
            A L    LA  +I++   +     +A Q     + R        + I G +  +V    
Sbjct: 520 PAELEALLLAHPEIADAAVIPIPDMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYK 554

Query: 559 LVKR----GSVPKTTSGKIRR 573
            +++     S+PK  SGKI R
Sbjct: 580 KIRKVTFLASIPKNPSGKILR 554

[Close]

BLAST of MC08g0677 vs. TAIR 10
Match: AT1G20490.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 64.7 bits (156), Expect = 3.0e-10
Identity = 89/406 (21.92%), Postives = 162/406 (39.90%), Query Frame = 0


Query: 42  AGAGSVLTYRQLHDSVQLISLRLLRQ--LRRRETVVILCWAGLDLVELIYGCQRAGLVSV 101
           A  G  LT+  L   V  ++  L  +  +RR + V+IL    + +  +       G V  
Sbjct: 58  ASTGHRLTFSDLWRVVDRVADCLYHEVGIRRGDVVLILSPNSIYIPVVCLSVMSLGAVVT 117

Query: 102 PIMPPDPRFTAQNKNYDLTRVLSQTKPKAAIAHQAY---IAAVFRYLSSTQRDENLATLL 161
                       N + ++++ ++Q+ P            +AA    + + + DE    L 
Sbjct: 118 TA-------NTLNTSGEISKQIAQSNPTLVFTTSQLAPKLAAAISVVLTDEEDEKRVELT 177

Query: 162 HNVKWISMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGA 221
             V+ + + S    KE          +        ++  ++ Y+SG TG  K V+    +
Sbjct: 178 SGVRVVGILSEMMKKETSGQRVRDRVNQ-------DDTAMMLYSSGTTGTSKGVI----S 237

Query: 222 AAHNVRAARRAY---DLHTDAVIVSWLPQYHDCGLM-FLLLTVTCGATCVLTSPGRFVGS 281
           +  N+ A    Y       D + V  +P +H  GL+ F + +V  G+T V+    RF   
Sbjct: 238 SHRNLTAYVAKYIDDKWKRDEIFVCTVPMFHSFGLLAFAMGSVASGSTVVILR--RFGLD 297

Query: 282 PMTWLHLVTAFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAV 341
            M  +  V  ++AT   +   A P++V        +    +L SLR +     P+ +  +
Sbjct: 298 DM--MQAVEKYKAT---ILSLAPPVLVAMINGADQLKAKYDLTSLRKVRCGGAPLSKEVM 357

Query: 342 EEFVEMFGSAGLEPGCVAPSYGLAENCTFVSTAWRKGWWFPAMPSHRKLLPCGWVGGEDE 401
           + F+E + +  +  G     Y L E+    ++            S  + L  G VG    
Sbjct: 358 DSFLEKYPTVNIFQG-----YALTESHGSGAST----------ESVEESLKYGAVGLLSS 417

Query: 402 EIVVAVVNGETGEVVDDDVEGEIWVSSPSNASGYLGNPSVTRETFH 439
            I   +V+ +TG V+  +  GE+W+  PS + GY GN   T ET +
Sbjct: 418 GIEARIVDPDTGRVMGVNQPGELWLKGPSISKGYFGNEEATNETIN 423

[Close]

BLAST of MC08g0677 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 64.3 bits (155), Expect = 3.9e-10
Identity = 118/495 (23.84%), Postives = 188/495 (37.98%), Query Frame = 0


Query: 11  PDQPVVDLYLPVWANLPAFRSKPAFVWSEDGAGAGSVLTYRQLHDSVQLISLRLLRQLRR 70
           P  P   L   ++ N  ++ SK A   S+     G  LT+ QL  +V  ++    R   R
Sbjct: 22  PKDPNTSLVSFLFRNSSSYPSKLAIADSD----TGDSLTFSQLKSAVARLAHGFHRLGIR 81

Query: 71  RETVVILCWAGLDLVELIYGCQRAGLVSVPIMPPDPRFTAQNKNYDLTRVLSQTK---PK 130
           +  VV++               +  L  + +      FT  N  Y +  V  Q K   PK
Sbjct: 82  KNDVVLI---------FAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPK 141

Query: 131 AAIAHQAYIAAVFRY----LSSTQRDENLATLLHNVKWISMESLKKPKELEEPDYSKFCH 190
             I+       +  +    +    +D        N K +S +++    EL EP       
Sbjct: 142 IIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNV---MELSEP----VSE 201

Query: 191 SSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNVRAARRAYDLHTD------AVIV 250
             +   K  +   + Y+SG TG  K V ++ G    N  AA     +  D       V +
Sbjct: 202 YPFVEIKQSDTAALLYSSGTTGTSKGVELTHG----NFIAASLMVTMDQDLMGEYHGVFL 261

Query: 251 SWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVTAFRATCTPVPCFALPL 310
            +LP +H  GL  +  +        L S  RF    +  L  +  FR T   V     P+
Sbjct: 262 CFLPMFHVFGLAVITYSQLQRGN-ALVSMARFELELV--LKNIEKFRVTHLWV---VPPV 321

Query: 311 VVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGSAGLEPGCVAPSYGLAE 370
            +  +K++  +    +L SL+ +     P+ +  +EE      +  L  G     YG+ E
Sbjct: 322 FLALSKQS--IVKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQG-----YGMTE 381

Query: 371 NCTFVSTAWRKGWWFPAMPSHRKLLPCGWVGGEDEEIVVAVVNGETGEVVDDDVEGEIWV 430
            C  VS         P +         G  G     +   +V+ ETG+    + +GEIWV
Sbjct: 382 TCGIVSVE------DPRLGKRNS----GSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWV 441

Query: 431 SSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVIKGADRFLFVIGRSSDIIK 490
             P+   GYL NP  T+ET   K        S+V TGD G     D  L+V+ R  ++IK
Sbjct: 442 RGPNMMKGYLNNPQATKETIDKK--------SWVHTGDLGYF-NEDGNLYVVDRIKELIK 459

Query: 491 LNSDQQIHPHYIESI 493
                Q+ P  +E +
Sbjct: 502 YKG-FQVAPAELEGL 459

[Close]

BLAST of MC08g0677 vs. TAIR 10
Match: AT5G38120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 63.2 bits (152), Expect = 8.8e-10
Identity = 75/307 (24.43%), Postives = 128/307 (41.69%), Query Frame = 0


Query: 192 EEPYLIQYTSGATGPPKAVVVSAG-AAAHNVRAARRAYDLHTDAVIVSWLPQYHDCGLM- 251
           ++  ++ Y+SG TG  K V  S G   AH  R     ++       +  +P +H  GL+ 
Sbjct: 199 DDTAMLLYSSGTTGRSKGVNSSHGNLIAHVARYIAEPFE-QPQQTFICTVPLFHTFGLLN 258

Query: 252 FLLLTVTCGATCVLTSPGRFVGSPMTWLHLVTAFRATCTPVPCFALPLVVKRAKENPGVA 311
           F+L T+  G T V+  P   +G  M     V  +RAT         P++V    +   + 
Sbjct: 259 FVLATLALGTTVVIL-PRFDLGEMMA---AVEKYRATTL---ILVPPVLVTMINKADQIM 318

Query: 312 GGVNLGSLRNLILINEPIYRSAVEEFVEMFGSAGLEPG-CVAPSYGLAENCTFVSTAWRK 371
              ++  LR +     P+ +   + F++ + +  +  G  +  S G   +   V  + R 
Sbjct: 319 KKYDVSFLRTVRCGGAPLSKEVTQGFMKKYPTVDVYQGYALTESNGAGASIESVEESRRY 378

Query: 372 GWWFPAMPSHRKLLPCGWVGGEDEEIVVAVVNGETGEVVDDDVEGEIWVSSPSNASGYLG 431
           G           LL CG        +   +V+  TG+V+  +  GE+W+  PS A GY  
Sbjct: 379 G--------AVGLLSCG--------VEARIVDPNTGQVMGLNQTGELWLKGPSIAKGY-- 438

Query: 432 NPSVTRETFHSKLRNNER---SRSFVRTGDRGVIKGADRFLFVIGRSSDIIKLNSDQQIH 491
                        RN E    S  +++TGD   I   D FLF++ R  ++IK     Q+ 
Sbjct: 439 ------------FRNEEEIITSEGWLKTGDLCYIDN-DGFLFIVDRLKELIKYKG-YQVP 465

Query: 492 PHYIESI 493
           P  +E++
Sbjct: 499 PAELEAL 465

The following BLAST results are available for this feature:

BLAST of MC08g0677 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B2HIL4	2.1e-40	27.87	4-hydroxyphenylalkanoate adenylyltransferase OS=Mycobacterium marinum (strain AT...	[more]
Q02278	3.6e-40	27.26	Long-chain-fatty-acid--AMP ligase FadD28 OS=Mycobacterium bovis (strain ATCC BAA...	[more]
P9WQ58	3.6e-40	27.26	Long-chain-fatty-acid--AMP ligase FadD28 OS=Mycobacterium tuberculosis (strain C...	[more]
P9WQ59	3.6e-40	27.26	Long-chain-fatty-acid--AMP ligase FadD28 OS=Mycobacterium tuberculosis (strain A...	[more]
Q7TXK5	1.5e-38	27.72	4-hydroxyphenylalkanoate adenylyltransferase OS=Mycobacterium bovis (strain ATCC...	[more]

BLAST of MC08g0677 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144296.1	0.0	100.00	uncharacterized protein LOC111014011 [Momordica charantia]	[more]
XP_023546350.1	3.81e-314	71.21	uncharacterized protein LOC111805474 [Cucurbita pepo subsp. pepo]	[more]
XP_022962563.1	4.18e-311	70.85	uncharacterized protein LOC111462961 [Cucurbita moschata]	[more]
KAG7029389.1	2.46e-309	70.70	fadD26, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6598448.1	7.85e-308	70.70	hypothetical protein SDJN03_08226, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of MC08g0677 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CSZ4	0.0	100.00	uncharacterized protein LOC111014011 OS=Momordica charantia OX=3673 GN=LOC111014...	[more]
A0A6J1HF51	2.02e-311	70.85	uncharacterized protein LOC111462961 OS=Cucurbita moschata OX=3662 GN=LOC1114629...	[more]
A0A6J1K7R6	5.02e-304	69.30	uncharacterized protein LOC111492429 OS=Cucurbita maxima OX=3661 GN=LOC111492429...	[more]
A0A0A0LL23	4.34e-298	69.13	AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G361690...	[more]
A5AZE1	4.92e-246	61.71	AMP-binding domain-containing protein OS=Vitis vinifera OX=29760 GN=VITISV_04165...	[more]

BLAST of MC08g0677 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G20500.1	1.7e-13	23.78	AMP-dependent synthetase and ligase family protein	[more]
AT1G20480.1	7.9e-11	23.88	AMP-dependent synthetase and ligase family protein	[more]
AT1G20490.1	3.0e-10	21.92	AMP-dependent synthetase and ligase family protein	[more]
AT4G05160.1	3.9e-10	23.84	AMP-dependent synthetase and ligase family protein	[more]
AT5G38120.1	8.8e-10	24.43	AMP-dependent synthetase and ligase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 29..478 e-value: 8.3E-73 score: 245.4
None	No IPR available	GENE3D	3.30.300.30		coord: 474..594 e-value: 6.9E-19 score: 70.3
None	No IPR available	PANTHER	PTHR22754:SF35	10A19I.5	coord: 1..598
None	No IPR available	PANTHER	PTHR22754	DISCO-INTERACTING PROTEIN 2 DIP2 -RELATED	coord: 1..598
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 23..581
IPR042099	ANL, N-terminal domain	GENE3D	3.40.50.12780		coord: 9..471 e-value: 6.4E-93 score: 314.0

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g0677.1	MC08g0677.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008610	lipid biosynthetic process
cellular_component	GO:0016020	membrane