MC08g0642 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g0642
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionglycosylphosphatidylinositol anchor attachment 1 protein-like
LocationMC08: 5204538 .. 5208969 (+)
RNA-Seq ExpressionMC08g0642
SyntenyMC08g0642
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ACACCAAAAACGAAGGCACGACCGATTCTGCGACTGGGGATTTTCCTGATTTCTCACAGTGTTTTCTTCAGGTACGCTGCTGGTTTTACGTGGATGTGATTCTCATTTCGTTTCAGAATATATTGATTGATTAATCTGGTCCTTTTTCTTTTTCGTTTTTGGTGCTCTCTAGCGTGATTTGCTTCTCCACGGGTTTCAGCTCTTTTGCTACTGCCAATGTTCGCTATGAACACCTACATTTCCGAGAACGCTCTCATGCCAGGTCATTTTCGCTTCTTGCTTAGCTTAGCTTAGCTTATCGTTATCTTATTTGTTTATTTATTACTCATCATTTTTATGATTTAGTGATTGATCGCCTGGTGGATAATTTACAAATTAAAATCAAAATTCATTAGTTGGGGAAGTATCTTAAATTGTAGGATTATCAATTTTATGCCTTGTTCTTTTGCAGTGATCTACTCTTTGTAATTTTATATATGGATACAGTACCCAAAGGTTGTTTAGAGCGTATATATGCTGGAGTACTCTTTAATTGTGGTTTGTGGAATTCTTCTATTGTCCCACAGGTTCTGCAAATACCATGCTTTCTGGTCAGGAAGTTTCAGAGGCGAACAACTTGGTGAAGGATCTAAAGGCTTTGAACTCGAAACCTGGTTCGACAGTCTTGTATGTAATTAGTGTCCCACTTTTCTCCATTTCTTTTTCCTCCTTTAACATACAATGGAACAATCTTGTGTAATGACTAAAATTTTGAGAATAATCACTTCAAATCAAAATTTGTCTACGTGGTTTGTTATGACTTTGTATGCATTGACATTAATATGGTTTAATGTCGCATGCTTCAAAAGGCATTTGTGCATTTTAACTCAGTCAACTATTTGACTAATTACAAATTGTCACTTTTTACAAATTGTCACTTTGCAACAAACTTTTGGAACTGCATCCTAAGCACTTTTGGGTGGACCACTGCACTACCGACTGATCCTCCCCTCCTTCTCGAAGTTACTCTTACAAGACACCCCTTCAAAAAGGAAAAGGCAATTCTTTGGCAAAACTTCATGAGAGCTTTCCTTTGGAACCTTTGGAGAGAACGGAATCAACGTAACTTCACAGACAAAACTCTCCCCTTTGATATCTTTTTTGAAGCTATAGTATTCAAAGTCATTACTTCGTGTAAATCCTCCCCTATGTTTCACTGCTATAGCTACTCTTCCCTCTTAGCAAATTGGAAGTGCTTCATGTAATCCCTCAATTTGAGGCTTTTGTAATTTCATACATCAATGAAATTGTTTCTTACACAAAAAAAAAACTATTTGACTAATTACAAGTATTTAAGTCAACAATGGTAGGGTGAAACTTTCAAGTTCACTTGTATGCGTAGTCCCATGAAATTATCAAGGAACCATCAACAAGCTCAATTGTGCCAATTGGAAAATATTTAAATATGTTATCCATTTAGCTTTCAATATGAACTTCTGAATGAGGCTGATATTCTTTGTCTGGAAAGGGTTTAGAATATTAATCCTAAAGTTCTTGTACAAGTTACGTGTATCAAAGGAAAGCTTTCATTAGAATGTGGTAGATGTTATTGCTTCAATAATTTAATGAAAGCTTTCATCAGAATGTGGTATATGATATTTCTTCAATAATTTTCTGATATGTGATGTCTATGCTTGTTTCATTTTATCTCTGACCATGATGTGCATGTGGATTGACATGCCATTTGATGTTTCTCAAGAGGGGCGTGGATGTGTAAAGATAATCATAGTCTTGATTGTGTGCAACTTTTTTCACTACTTACTTTGCAGTGCACTGCATAGTTATCTAATTCATCTTCCACAGAATTTATCAATAGCATCTTTTTCACATTTCAATATGCATCATCTTATTCTTCTCCTTGCCTCTGTTTGCATTGCAAGTCTTTTGATTTTCTATCCATCTTTACAGTGGAATTCAGCAGATTCTAGCACAATATATATCAGACTTGGGTGCTGAAGTTAATTACCACAAGTTCCACCCTCAGTCTGGTCAGTTCCATCCATTGCACTTTTTCTCTAGCCCTGATTCTGGTATCATTCGGGACAATGTTAGTTGCATGGCACAGGTATTAACACTGTTGGCATAATAAGAGCACCACAAGCCGATGGAAAGGAAGCAATCGTCTTGGTGACAACTTACAATCCTGTTAAGACTAGTCTTTATGAGACTTTGTCTCTTGGTATTGCATATTCAGTATTTTCTTTGCTTACATAAGTAACTTGGTTGGCCAAAGACATTGTATGGCTCATCGTGGATTCACAATATGGGGAATATGCTGCAGTTTCAGCTTGGCTGAGAGACTATCACACTCCTGCATTTTGGTCGATCCGGCATCATTGACAAAGATGCTTGTGCTGAGATGAGTGTCCTTAATAAATTAGAAACAAACCATATGACAGAAAAAATGAGTCTAGATGATTTTAAACGTGCTGGAACAATGGCTGCAGCACTTGTTATTAAGGTTTCAAATAAAAGTGAACGCTTTGAGGATAGTCTTAGTGTCTATGCTGAAGCATCTAATGGTCAGATGCCGAACCTGGATCTCATCAATTTGTGAATTATATGGCAGTACATAGGCAAGGTTTTTGGATTAAGGTTGAGAAATTTTGGCCTTTACTAGACTGCAAATGGCTCAAGGTTTTGGGTGAAGTGTTTGAGTCAATAGGACAATTGATCCGAAGCTTGAACTCTGAATGGAAGTTTGGCATCCTTGCTTCTGACTATGTTGATGGCACTGCCACACTTGCAAGTTCATTGTACTACCAGGTGGTTTACTTTTCAGTTTATTTGCCAAGAGCTATATGGTTTCATTTAATGAACAAGAATAAAGAAGGTTTTTTCATTTATGTTAATAATAAAGATTACATCCACTTAAGTTAAACCATGTTCTTCTTGCCATTAGGCTGTGGGTATTCCTACTGGTCCCCATGGGGCTTTTCGAGATTACCAAGTTGATGCAATTACCATGGAGATGTCCCCCAAATTTTCTTCTGGTATCAAGGTCAGGCATGATGAATTCTATTGCGAGGTGGCAGGTCAGTGCTCATTTATGCCCCAAGGATTTTGTAACACATCCTCCTCCCACCCCTCAACTTTTCATTTAGGGCTCACTGTATGAACGAATGTTTAAAACTAATGGTTTCCCTCAATGGGGTTAGCTTGACATCAAGTAATATTTTTTATTTTTTTATTATTTATTATTTCCCATTTTTTAACTTCGCGTTTTACTGTCAATCTTCCATGTTTAAATTTTAGTTTGTAAATAGTTAAACTGCAGTAAGAGAAAATTTGTTTTTTTTTAACAATGAAACTTTGGCTAATAGTTTCTCTTTTTAAACGAATAGTGATAAATTCATTCAAGCAAATTTATTGCCTAAAGTCATGTTCATGTACGAAACTGGGTTTAGATTTTGGTGTATTAAATGATCAAGATATTCATAATTTTAATCTGGAATTAAAATGGTAGGACTAAAATTCTCCAAGTCTTTTTAATGGCTAAGGATTTTGTAGTTGGGGTTGGTGCAGGCTAATTGAAGGAGTTGTACGATCAGTAAACAACCTCCTGGAAAAGTTCCATCAGTCATTCTTCCTATACCTTATGGTATCTACAAGCAAATTTGTATCAGTTGGCGTATATATGATTGCTTTTGCCCTCCTTGTTGCTCCACTACCAGCTGTTGCGGCTTCTCTCTACTCTTATGCAAACAATTTGAATTTAACCGTGGAAAAGGTCGAACATGCAGTTCCAGCAAATCCTGATGATGAGCTTATTGTCTCTTTGAGATCATGGAAATGGCTTAATGCTGCAAAAAATGTTTTTGTTGTTCATGTATGGGGTGCTGTTGTTTCTTTACTTCCCTACTTCATCTGCCATTTACCTGGTTACAGTCCTACAACAAGCTCTACTATTTGGGCGTTGCTTTCCCTGCTCCCCCTACTAATTTCGTCCGTGATTTTGGGCTTGCCATTCACTTGCACCAAACCTCCTCACCAGCTTATTCAAGAGTGCGCTTTCCTGAAAGCAATGACCATCTCAGCGGCCTTCATTGGTTTGTGCCTCATGTCAGTAATTAACTTTGCTACTGCAGAACTTGGAGCATTTTTTATGGTTTCCATGTGCTTGATGGCACATCCTCTGAAGCTTGATCTAATGGCTGGGAGTTTTAAAGCTCTTTCAAGGGCAGCCTGTAACATTGTTTTGGGATCCATAGCTTTTCCCCCTATTGCTTTCTTTTTGTTTAAAGGTTCTTTGCAAGGTTTCCACAATCTACATATTGGCGACTTCTGGAACTGGATGGAGACCCTCTGGGCTTGGAACAGTGCTACTTTCCTCTACTTAGGTATGGTCCATCTGCCATGTTGGGCATTATGCATACAAATTTTACTTCATCCT

mRNA sequence

ACACCAAAAACGAAGGCACGACCGATTCTGCGACTGGGGATTTTCCTGATTTCTCACAGTGTTTTCTTCAGCGTGTTTGCTTCTCCACGGGTTTCAGCTCTTTTGCTACTGCCAATGTTCGCTATGAACACCTACATTTCCGAGAACGCTCTCATGCCAGGTTCTGCAAATACCATGCTTTCTGGTCAGGAAGTTTCAGAGGCGAACAACTTGGTGAAGGATCTAAAGGCTTTGAACTCGAAACCTGGTTCGACAGTCTTTGGAATTCAGCAGATTCTAGCACAATATATATCAGACTTGGGTGCTGAAGTTAATTACCACAAGTTCCACCCTCAGTCTGGTCAGTTCCATCCATTGCACTTTTTCTCTAGCCCTGATTCTGGTATCATTCGGGACAATGTTAGTTGCATGGCAGGTATTAACACTGTTGGCATAATAAGAGCACCACAAGCCGATGGAAAGGAAGCAATCGTCTTGGTGACAACTTACAATCCTGTTAAGACTAGTCTTTATGAGACTTTGTCTCTTGGTATTGCATATTCAGTATTTTCTTTGCTTACATAAGTAACTTGGTTGGCCAAAGACATTGTATGGCTCATCGTGGATTCACAATATGGGGAATATGCTGCAGTTTCAGCTTGGCTGAGAGACTATCACACTCCTGCATTTTGTGTCCTTAATAAATTAGAAACAAACCATATGACAGAAAAAATGAGTCTAGATGATTTTAAACGTGCTGGAACAATGGCTGCAGCACTTGTTATTAAGGTTTCAAATAAAAGTGAACGCTTTGAGGATAGTCTTAGTGTCTATGCTGAAGCATCTAATGGTCAGATGCCGAACCTGGATCTCATCAATGTGAATTATATGGCAGTACATAGGCAAGGTTTTTGGATTAAGGTTGAGAAATTTTGGCCTTTACTAGACTGCAAATGGCTCAAGGTTTTGGGTGAAGTGTTTGAGTCAATAGGACAATTGATCCGAAGCTTGAACTCTGAATGGAAGTTTGGCATCCTTGCTTCTGACTATGTTGATGGCACTGCCACACTTGCAAGTTCATTGTACTACCAGGCTGTGGGTATTCCTACTGGTCCCCATGGGGCTTTTCGAGATTACCAAGTTGATGCAATTACCATGGAGATGTCCCCCAAATTTTCTTCTGGTATCAAGGTCAGGCATGATGAATTCTATTGCGAGGTGGCAGGTCAGCTAATTGAAGGAGTTGTACGATCAGTAAACAACCTCCTGGAAAAGTTCCATCAGTCATTCTTCCTATACCTTATGGTATCTACAAGCAAATTTGTATCAGTTGGCGTATATATGATTGCTTTTGCCCTCCTTGTTGCTCCACTACCAGCTGTTGCGGCTTCTCTCTACTCTTATGCAAACAATTTGAATTTAACCGTGGAAAAGGTCGAACATGCAGTTCCAGCAAATCCTGATGATGAGCTTATTGTCTCTTTGAGATCATGGAAATGGCTTAATGCTGCAAAAAATGTTTTTGTTGTTCATGTATGGGGTGCTGTTGTTTCTTTACTTCCCTACTTCATCTGCCATTTACCTGGTTACAGTCCTACAACAAGCTCTACTATTTGGGCGTTGCTTTCCCTGCTCCCCCTACTAATTTCGTCCGTGATTTTGGGCTTGCCATTCACTTGCACCAAACCTCCTCACCAGCTTATTCAAGAGTGCGCTTTCCTGAAAGCAATGACCATCTCAGCGGCCTTCATTGGTTTGTGCCTCATGTCAGTAATTAACTTTGCTACTGCAGAACTTGGAGCATTTTTTATGGTTTCCATGTGCTTGATGGCACATCCTCTGAAGCTTGATCTAATGGCTGGGAGTTTTAAAGCTCTTTCAAGGGCAGCCTGTAACATTGTTTTGGGATCCATAGCTTTTCCCCCTATTGCTTTCTTTTTGTTTAAAGGTTCTTTGCAAGGTTTCCACAATCTACATATTGGCGACTTCTGGAACTGGATGGAGACCCTCTGGGCTTGGAACAGTGCTACTTTCCTCTACTTAGGTATGGTCCATCTGCCATGTTGGGCATTATGCATACAAATTTTACTTCATCCT

Coding sequence (CDS)

ACACCAAAAACGAAGGCACGACCGATTCTGCGACTGGGGATTTTCCTGATTTCTCACAGTGTTTTCTTCAGCGTGTTTGCTTCTCCACGGGTTTCAGCTCTTTTGCTACTGCCAATGTTCGCTATGAACACCTACATTTCCGAGAACGCTCTCATGCCAGGTTCTGCAAATACCATGCTTTCTGGTCAGGAAGTTTCAGAGGCGAACAACTTGGTGAAGGATCTAAAGGCTTTGAACTCGAAACCTGGTTCGACAGTCTTTGGAATTCAGCAGATTCTAGCACAATATATATCAGACTTGGGTGCTGAAGTTAATTACCACAAGTTCCACCCTCAGTCTGGTCAGTTCCATCCATTGCACTTTTTCTCTAGCCCTGATTCTGGTATCATTCGGGACAATGTTAGTTGCATGGCAGGTATTAACACTGTTGGCATAATAAGAGCACCACAAGCCGATGGAAAGGAAGCAATCGTCTTGGTGACAACTTACAATCCTGTTAAGACTAGTCTTTATGAGACTTTGTCTCTTGGTATTGCATATTCAGTATTTTCTTTGCTTACATAAGTAACTTGGTTGGCCAAAGACATTGTATGGCTCATCGTGGATTCACAATATGGGGAATATGCTGCAGTTTCAGCTTGGCTGAGAGACTATCACACTCCTGCATTTTGTGTCCTTAATAAATTAGAAACAAACCATATGACAGAAAAAATGAGTCTAGATGATTTTAAACGTGCTGGAACAATGGCTGCAGCACTTGTTATTAAGGTTTCAAATAAAAGTGAACGCTTTGAGGATAGTCTTAGTGTCTATGCTGAAGCATCTAATGGTCAGATGCCGAACCTGGATCTCATCAATGTGAATTATATGGCAGTACATAGGCAAGGTTTTTGGATTAAGGTTGAGAAATTTTGGCCTTTACTAGACTGCAAATGGCTCAAGGTTTTGGGTGAAGTGTTTGAGTCAATAGGACAATTGATCCGAAGCTTGAACTCTGAATGGAAGTTTGGCATCCTTGCTTCTGACTATGTTGATGGCACTGCCACACTTGCAAGTTCATTGTACTACCAGGCTGTGGGTATTCCTACTGGTCCCCATGGGGCTTTTCGAGATTACCAAGTTGATGCAATTACCATGGAGATGTCCCCCAAATTTTCTTCTGGTATCAAGGTCAGGCATGATGAATTCTATTGCGAGGTGGCAGGTCAGCTAATTGAAGGAGTTGTACGATCAGTAAACAACCTCCTGGAAAAGTTCCATCAGTCATTCTTCCTATACCTTATGGTATCTACAAGCAAATTTGTATCAGTTGGCGTATATATGATTGCTTTTGCCCTCCTTGTTGCTCCACTACCAGCTGTTGCGGCTTCTCTCTACTCTTATGCAAACAATTTGAATTTAACCGTGGAAAAGGTCGAACATGCAGTTCCAGCAAATCCTGATGATGAGCTTATTGTCTCTTTGAGATCATGGAAATGGCTTAATGCTGCAAAAAATGTTTTTGTTGTTCATGTATGGGGTGCTGTTGTTTCTTTACTTCCCTACTTCATCTGCCATTTACCTGGTTACAGTCCTACAACAAGCTCTACTATTTGGGCGTTGCTTTCCCTGCTCCCCCTACTAATTTCGTCCGTGATTTTGGGCTTGCCATTCACTTGCACCAAACCTCCTCACCAGCTTATTCAAGAGTGCGCTTTCCTGAAAGCAATGACCATCTCAGCGGCCTTCATTGGTTTGTGCCTCATGTCAGTAATTAACTTTGCTACTGCAGAACTTGGAGCATTTTTTATGGTTTCCATGTGCTTGATGGCACATCCTCTGAAGCTTGATCTAATGGCTGGGAGTTTTAAAGCTCTTTCAAGGGCAGCCTGTAACATTGTTTTGGGATCCATAGCTTTTCCCCCTATTGCTTTCTTTTTGTTTAAAGGTTCTTTGCAAGGTTTCCACAATCTACATATTGGCGACTTCTGGAACTGGATGGAGACCCTCTGGGCTTGGAACAGTGCTACTTTCCTCTACTTAGGTATGGTCCATCTGCCATGTTGGGCATTATGCATACAAATTTTACTTCATCCT

Protein sequence

TPKTKARPILRLGIFLISHSVFFSVFASPRVSALLLLPMFAMNTYISENALMPGSANTMLSGQEVSEANNLVKDLKALNSKPGSTVFGIQQILAQYISDLGAEVNYHKFHPQSGQFHPLHFFSSPDSGIIRDNVSCMAGINTVGIIRAPQADGKEAIVLVTTYNPVKTSLYETLSLGIAYSVFSLLTUVTWLAKDIVWLIVDSQYGEYAAVSAWLRDYHTPAFCVLNKLETNHMTEKMSLDDFKRAGTMAAALVIKVSNKSERFEDSLSVYAEASNGQMPNLDLINVNYMAVHRQGFWIKVEKFWPLLDCKWLKVLGEVFESIGQLIRSLNSEWKFGILASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITMEMSPKFSSGIKVRHDEFYCEVAGQLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTVEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHVWGAVVSLLPYFICHLPGYSPTTSSTIWALLSLLPLLISSVILGLPFTCTKPPHQLIQECAFLKAMTISAAFIGLCLMSVINFATAELGAFFMVSMCLMAHPLKLDLMAGSFKALSRAACNIVLGSIAFPPIAFFLFKGSLQGFHNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHP
Homology
BLAST of MC08g0642 vs. ExPASy Swiss-Prot
Match: Q9WTK3 (Glycosylphosphatidylinositol anchor attachment 1 protein OS=Mus musculus OX=10090 GN=Gpaa1 PE=1 SV=3)

HSP 1 Score: 128.6 bits (322), Expect = 2.7e-28
Identity = 163/668 (24.40%), Postives = 275/668 (41.17%), Query Frame = 0

Query: 35  LLLPMFAMNTYISENALMPGSANTMLSGQEV--SEANNLVKDLKALNSKPGSTVFGIQQI 94
           L  P     TY+SENA+     +TM+  Q V    A +  +D  A   KPG+        
Sbjct: 39  LAFPPLTQRTYMSENAM----GSTMVEEQFVGGDRARSFARDFAAHRKKPGALPVA---W 98

Query: 95  LAQYISDLGAEVNYHKFHPQSGQFHPLHFFSSPDSGIIRDNVSCMAGINTVGIIRAPQAD 154
           L + +  +G EV    F  +            PD    R  VS   G N  GI+RAP++ 
Sbjct: 99  LERSMRSVGLEVYTQSFSRK---------LPFPDETHERYMVS---GTNVYGILRAPRSA 158

Query: 155 GKEAIVLVTTYNPVKTSLYETLSLGIAYSVFSLLTXVTWLAKDIVWLIVDSQYGEYAAVS 214
             E++VL     P  T+   + ++G+  ++ +      + AKDI++L+ D    +     
Sbjct: 159 STESLVLTVPCGPDATN---SQAVGLLLALAAHFRGQIYWAKDIIFLVTDH---DLLGTE 218

Query: 215 AWLRDYHTPAFCVLNKLETNHMTEKMSLDDFKRAGTMAAALVIKVSNKSERFEDSLSVYA 274
           AWL  YH             ++T   S     RAG + AA+ +++S+       SL V  
Sbjct: 219 AWLEAYH-----------DINVTGIQSSPLQGRAGAIQAAVALELSSD---VVTSLDVTV 278

Query: 275 EASNGQMPNLDLINVNYMAVHRQGFWIKVEKFWPLLDCKWLKVLGEVFESIGQLIRSLNS 334
           E  NGQ+PNLDL+N+      + G    ++    L    W  + G               
Sbjct: 279 EGLNGQLPNLDLLNLFQTFCQKGGLLCTLQ--GKLQPQDWTSLEGP-------------- 338

Query: 335 EWKFGILASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITMEMSPKFSSGIKVR 394
                      + G  TL   +  QA G P GPHG F  Y V+A+T+     F      R
Sbjct: 339 -----------LQGLQTLLLMVLRQASGRPHGPHGLFLRYGVEALTLRGINSF------R 398

Query: 395 HDEFYCEVAGQLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLP 454
             ++     G+ +EG+ R +N+LLE+ HQSFF YL+ + S+FVS+G+YM A   L+  L 
Sbjct: 399 QYKYDLATVGKALEGMFRKLNHLLERLHQSFFFYLLPALSRFVSIGLYMPATGFLLLVLG 458

Query: 455 AVAASLYSYANNLNLTVEKVEHA-VPANPDDELIVSLRSWKWLNAAKNVFVVHVWGAVVS 514
             A  L+   +   +  E+   A  P  P    ++  +     +    + +    G  + 
Sbjct: 459 LKALELWMQLHQAGVNPEEAGKAPSPGTP----LLPTQGVGLASLTAPLLISQAMGLALY 518

Query: 515 LLPYFICHLPGYSPTTSSTIWALLSLLPLLISSVILGLPFTCTKPPHQLIQECAFLKAMT 574
            LP    HL       +     +L+LL + ++   L LP    +  +  + +  ++    
Sbjct: 519 FLPVLGQHLATQHFPVAEAEAVVLTLLAIYVAG--LALPHNTHRVVNSQVPDRGWMALKL 578

Query: 575 ISAAFIGLCL--MSVINFATAELGAFFMVSMCLMAHPLKLDLMAGSFKALSRAACNIVLG 634
           ++  ++ L L  ++++NF+   L A  MV    +A P     +  +   ++  A  +  G
Sbjct: 579 VALIYLALQLGCIALLNFSLGFLLAATMVPAAALAKPHGPRTLYAALLVVTSPAVTL-FG 618

Query: 635 SIAFPPIAFFLFKGSLQGFHNLHIGDFWNWMETL--------WAWNSATFLYLGMVHLPC 690
           S+       FL++  L+    L + + W    T         + + +  F  L +   PC
Sbjct: 639 SL-------FLWRELLE--VPLSLAEGWQLFLTALAQGVLEHYTYGALLFPILALGLYPC 618

BLAST of MC08g0642 vs. ExPASy Swiss-Prot
Match: O43292 (Glycosylphosphatidylinositol anchor attachment 1 protein OS=Homo sapiens OX=9606 GN=GPAA1 PE=1 SV=3)

HSP 1 Score: 115.2 bits (287), Expect = 3.0e-24
Identity = 166/699 (23.75%), Postives = 278/699 (39.77%), Query Frame = 0

Query: 5   KARPILRLGIFLISHSVFFSVFASPRVSALLLLPMFAMNTYISENALMPGSANTMLSGQE 64
           + R + RL + L +     S  A       L+ P     TY+SENA+         +G +
Sbjct: 9   RRRALARLVLRLNAPLCVLSYVAGIAWFLALVFPPLTQRTYMSENAMGSTMVEEQFAGGD 68

Query: 65  VSEANNLVKDLKALNSKPGSTVFGIQQILAQYISDLGAEVNYHKFHPQSGQFHPLHFFSS 124
              A    +D  A   K G+        L + +  +G EV    F  +            
Sbjct: 69  --RARAFARDFAAHRKKSGALPVA---WLERTMRSVGLEVYTQSFSRK---------LPF 128

Query: 125 PDSGIIRDNVSCMAGINTVGIIRAPQADGKEAIVLVTTYNPVKTSLYETLSLGIAYSVFS 184
           PD    R  VS   G N  GI+RAP+A   E++VL     P  +    + ++G+  ++ +
Sbjct: 129 PDETHERYMVS---GTNVYGILRAPRAASTESLVLTV---PCGSDSTNSQAVGLLLALAA 188

Query: 185 LLTXVTWLAKDIVWLIVDSQYGEYAAVSAWLRDYHTPAFCVLNKLETNHMTEKMSLDDFK 244
                 + AKDIV+L+ +    +     AWL  YH             ++T   S     
Sbjct: 189 HFRGQIYWAKDIVFLVTEH---DLLGTEAWLEAYHDV-----------NVTGMQSSPLQG 248

Query: 245 RAGTMAAALVIKVSNKSERFEDSLSVYAEASNGQMPNLDLINVNYMAVHRQGFWIKVEKF 304
           RAG + AA+ +++S+       SL V  E  NGQ+PNLDL+N+      + G    ++  
Sbjct: 249 RAGAIQAAVALELSSD---VVTSLDVAVEGLNGQLPNLDLLNLFQTFCQKGGLLCTLQ-- 308

Query: 305 WPLLDCKWLKVLGEVFESIGQLIRSLNSEWKFGILASDYVDGTATLASSLYYQAVGIPTG 364
             L    W  + G                          + G  TL   +  QA G P G
Sbjct: 309 GKLQPEDWTSLDGP-------------------------LQGLQTLLLMVLRQASGRPHG 368

Query: 365 PHGAFRDYQVDAITMEMSPKFSSGIKVRHDEFYCEVAGQLIEGVVRSVNNLLEKFHQSFF 424
            HG F  Y+V+A+T+     F      R  ++     G+ +EG+ R +N+LLE+ HQSFF
Sbjct: 369 SHGLFLRYRVEALTLRGINSF------RQYKYDLVAVGKALEGMFRKLNHLLERLHQSFF 428

Query: 425 LYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTVEKVEHA----VPANP 484
           LYL+   S+FVS+G+YM A   L+  L   A  L+   +   + +E+   A    VP  P
Sbjct: 429 LYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLEEPGGAPGPSVPLPP 488

Query: 485 DDELIVSLRSWKWLNAAKNVFVVHVWGAVVSLLPYFICHLPGYSPTTSSTIWALLSLLPL 544
              + ++       +    + +    G  + +LP    H+       +     +L+LL +
Sbjct: 489 SQGVGLA-------SLVAPLLISQAMGLALYVLPVLGQHVATQHFPVAEAEAVVLTLLAI 548

Query: 545 LISSVILGLPFTCTKPPHQLIQECAFLKAMTISAAFIGLCL--MSVINFATAELGAFFMV 604
             +   L LP    +       +  ++    ++  ++ L L  +++ NF+   L A  MV
Sbjct: 549 YAAG--LALPHNTHRVVSTQAPDRGWMALKLVALIYLALQLGCIALTNFSLGFLLATTMV 608

Query: 605 SMCLMAHPLKLDLMAGSFKALSRAACNIVLGSIAFPPIAFFLFKGSLQGFHNLHIGDFWN 664
               +A P     +  +   L+  A  + LGS+       FL++   +    L + + W 
Sbjct: 609 PTAALAKPHGPRTLYAALLVLTSPAATL-LGSL-------FLWRELQEA--PLSLAEGWQ 618

Query: 665 WMETLWA--------WNSATFLYLGMVHLPCWALCIQIL 690
                 A        + +  F  L +   PCW L   +L
Sbjct: 669 LFLAALAQGVLEHHTYGALLFPLLSLGLYPCWLLFWNVL 618

BLAST of MC08g0642 vs. ExPASy Swiss-Prot
Match: Q9US48 (GPI transamidase component gaa1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gaa1 PE=3 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 1.7e-14
Identity = 132/538 (24.54%), Postives = 218/538 (40.52%), Query Frame = 0

Query: 139 GINTVGIIRAPQADGKEAIVLVTTYNPVKTSLYETLSLGIAYSVFSLLTXVTWLAKDIVW 198
           G N +  +RAP+ D  E+++L   +       Y    + +A S+       +  +KDI+ 
Sbjct: 123 GSNFITTLRAPRGDATESLLLCVPWKD-HIGQYNEAGVALAISLLKYFQGWSLWSKDIIL 182

Query: 199 LIVDSQ-YGEYAAVSAWLRDYHTPAFCVLNKLETNHMTEKMSLDDFK-RAGTMAAALVIK 258
           +I D   YG     S++L  Y             +  T  +S    K R+G++ A L ++
Sbjct: 183 VIFDDPVYGP----SSFLTSYF------------DQTTPYISYTPLKIRSGSIQAGLSLE 242

Query: 259 VSNKSERFEDSLSVYAEASNGQMPNLDLINVNYMAVHRQGFWIKVEKFWPLLDCKWLKVL 318
           +   +E   D L V  +A+NGQ+PNLDL N     +  Q F       +P      L++ 
Sbjct: 243 LVT-TENNSDVLEVLYQATNGQLPNLDLFN-TISRIFMQHF------NYP------LRLQ 302

Query: 319 GEVFESIGQLIRSLNSEWKFGILASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQVDA 378
           G  F +        NS        S Y     +L   +  QAV   T  H  F  Y++D 
Sbjct: 303 GYDFHA--------NS-------GSSYTSRLKSLWMGMLTQAVSNVTSAHALFPQYRIDM 362

Query: 379 ITMEMSPKFSSGIKVRHDEFYCEV--AGQLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKF 438
           +T+ M  K         D F  ++   GQ IE   RS+NNLLE  HQSFF Y ++    F
Sbjct: 363 LTLRMKVK---------DPFSFDMFRFGQAIESTFRSLNNLLEHLHQSFFFYFILDHLHF 422

Query: 439 VSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTVEKVEHAVPANPDDELIVSLRSWKWL 498
           +S+G YM +  +L     A +  L +Y +                             W+
Sbjct: 423 ISIGNYMPSILIL-----AASFMLGAYRH-----------------------------WI 482

Query: 499 NAAKNVFVVHVWGAVVSLLPYFICHLPGYSPTTSSTIWALLSLLPLLISSV-ILGLPFTC 558
           N  K    + +W      L    C +  Y   +SST   +   L L+++ + I+   F  
Sbjct: 483 NHEKK---IDLWRPFSFWLFSIFCTIAAYYLVSSSTKITVFIFLYLMLTFIGIIFSTFMT 542

Query: 559 TKPPHQLIQECAFLKAMTISAAFIGLCLMSVINFATAELGAFFMVSMCLMAHPLKLDLMA 618
           ++    ++      K++ IS       ++S +NF+ +     F+V++ L+  PL+     
Sbjct: 543 SEDAELVLSYDLMSKSLFIS-------VVSTLNFSLS-----FVVAILLV--PLQFI--- 543

Query: 619 GSFKALSRAACNIVLGSIAFPPIAF---FLFKGSLQGFHNLHIGDFWNWMETLWAWNS 669
            SF+   R        S+ F  + +   F+F  SL    N  +  FW W +    +NS
Sbjct: 603 -SFRFNRRL-------SLLFAVLTYFSTFIFLCSLSKILNGPLVPFWLWAKEYELFNS 543

BLAST of MC08g0642 vs. ExPASy Swiss-Prot
Match: P39012 (GPI transamidase component GAA1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=GAA1 PE=1 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 1.7e-14
Identity = 111/441 (25.17%), Postives = 186/441 (42.18%), Query Frame = 0

Query: 33  ALLLLPMFAM--NTYISENALMPGSANTMLSGQEVSEANNLVKDLKALNSKPGSTVFGIQ 92
           ++ +LPM      TYISENALMP  A +       SE N L      +      T     
Sbjct: 38  SIAILPMDGQYRRTYISENALMPSQAYSYF---RESEWNILRGYRSQIKEMVNMTSMERN 97

Query: 93  QILAQYISDLGAEVNYHKFHPQSGQFHPLHFFSSPDSGIIRDNVSCMAGINTVGIIRAPQ 152
            ++  ++ + G +   +    ++ Q+                      G    G++ AP+
Sbjct: 98  NLMGSWLQEFGTKTAIY----ENEQY----------------------GETLYGVMHAPR 157

Query: 153 ADGKEAIVL-VTTYNPVKTSLYETLSLGIAYSVFSLLTXVTWLAKDIVW---LIVDSQYG 212
            DG EA+VL V  +N          +LG++ + F         ++  VW   +IV     
Sbjct: 158 GDGTEAMVLAVPWFNSDDEFNIGGAALGVSLARF--------FSRWPVWSKNIIVVFSEN 217

Query: 213 EYAAVSAWLRDYHTPAFCVLNKLETNHMTEKMSLDDFKRAGTMAAALVIKVSNKSERFED 272
             AA+ +W+  YHT                  SLD     G++ AA+V+  S+ +E F +
Sbjct: 218 PRAALRSWVEAYHT------------------SLD--LTGGSIEAAVVLDYSS-TEDFFE 277

Query: 273 SLSVYAEASNGQMPNLDLINVNYMAVHRQGFWIKVEKFWPLLDCKWLKVLGEVFESIGQL 332
            + +  +  NG++PNLDL+N+       +G                +KV           
Sbjct: 278 YVEISYDGLNGELPNLDLVNIAISITEHEG----------------MKV----------- 337

Query: 333 IRSLNSEWKFGILASDYVDGTATLASSLYYQAVGIPTGPHG--AFRDYQVDAITMEMSPK 392
             SL+      +  +++      L   +   A+     PHG  AF  +++ ++T++    
Sbjct: 338 --SLHGLPSDQLTNNNFWSRLKILCLGIRDWALSGVKKPHGNEAFSGWRIQSVTLKAHG- 379

Query: 393 FSSGIKVRHDEFYCEVAGQLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYM-IA 452
            +SG    HD       G++ E + RS+NNLLEKFHQSFF YL+++  +FVS+  Y+  A
Sbjct: 398 -NSG----HD---ITTFGRIPEAMFRSINNLLEKFHQSFFFYLLLAPRQFVSISSYLPSA 379

Query: 453 FALLVAPLPAVAASLYSYANN 465
            AL +A      +SL ++ NN
Sbjct: 458 VALSIA---FAISSLNAFINN 379

BLAST of MC08g0642 vs. NCBI nr
Match: XP_022133688.1 (LOW QUALITY PROTEIN: glycosylphosphatidylinositol anchor attachment 1 protein-like [Momordica charantia])

HSP 1 Score: 1245 bits (3222), Expect = 0.0
Identity = 651/709 (91.82%), Postives = 662/709 (93.37%), Query Frame = 0

Query: 1   TPKTKARPILRLGIFLISHSVFFSVFA-SPRVSALLLLPMFAMNTYISENALMPGSANTM 60
           TPK KARPI+RLGIFLISHSVFFSV   S  V ALLLLPMFAMNTYISENALMPGSANTM
Sbjct: 6   TPKAKARPIVRLGIFLISHSVFFSVICFSAGVLALLLLPMFAMNTYISENALMPGSANTM 65

Query: 61  LSGQEVSEANNLVKDLKALNSKPGSTVFGIQQILAQYISDLGAEVNYHKFHPQSGQFHPL 120
           LSGQEVSEANNLVKD+KALNSKPGSTVFG QQILAQYISDLGAEVNYHKFHPQSGQFHPL
Sbjct: 66  LSGQEVSEANNLVKDIKALNSKPGSTVFGSQQILAQYISDLGAEVNYHKFHPQSGQFHPL 125

Query: 121 HFFSSPDSGIIRDNVSCMA-GINTVGIIRAPQADGKEAIVLVTTYNPVKTSLYETLSLGI 180
           HFFSSPDSGIIRDNVSCMA GINTVGII+APQADGKEAIVLVTTYNPVKTS YETLSLGI
Sbjct: 126 HFFSSPDSGIIRDNVSCMAQGINTVGIIKAPQADGKEAIVLVTTYNPVKTSXYETLSLGI 185

Query: 181 AYSVFSLLTXVTWLAKDIVWLIVDSQYGEYAAVSAWLRDYHTPAF--------------C 240
           AYSVFSLLTXVTWLAKDIVWLIVDSQYGEYAAVSAWLRDY+TPAF               
Sbjct: 186 AYSVFSLLTXVTWLAKDIVWLIVDSQYGEYAAVSAWLRDYYTPAFGRSGIIDKDACAEMS 245

Query: 241 VLNKLETNHMTEKMSLDDFKRAGTMAAALVIKVSNKSERFEDSLSVYAEASNGQMPNLDL 300
           VLNKL TNHMTEKMSLD+FKRAGTMAAALVIKVSNKSE FEDSLSVYAEASNGQMPNLDL
Sbjct: 246 VLNKLGTNHMTEKMSLDEFKRAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQMPNLDL 305

Query: 301 IN-VNYMAVHRQGFWIKVEKFWPLLDCKWLKVLGEVFESIGQLIRSLNSEWKFGILASDY 360
           IN VNYMAVHRQGF IK+EKFWPLLDCKWLKVLGEVFESIGQLIR LN EWKFGI ASDY
Sbjct: 306 INIVNYMAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGQLIRILNPEWKFGIPASDY 365

Query: 361 VDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITMEMSPKFSSGIKVRHDEFYCEVAGQ 420
           VDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAIT+EMSPKFSSGIKVR DEF     G+
Sbjct: 366 VDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITVEMSPKFSSGIKVRRDEFILR-GGR 425

Query: 421 LIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYAN 480
           LIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYAN
Sbjct: 426 LIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYAN 485

Query: 481 NLNLTVEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHVWGAVVSLLPYFICHLPGY 540
           NLNLT EKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVH WGAVVSLLPYFIC +PGY
Sbjct: 486 NLNLTXEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHXWGAVVSLLPYFICXJPGY 545

Query: 541 SPTTSSTIWALLSLLPLLISSVILGLPFTCTKPPHQLIQECAFLKAMTISAAFIGLCLMS 600
           SPTTS TIWALLSLL LLI SVILG PFTCTKP HQLIQECAFLKAMTISAAFIGLCLMS
Sbjct: 546 SPTTSXTIWALLSLLXLLIXSVILGXPFTCTKPXHQLIQECAFLKAMTISAAFIGLCLMS 605

Query: 601 VINFATAELGAFFMVSMCLMAHPLKLDLMAGSFKALSRAACNIVLGSIAFPPIAFFLFKG 660
           VINFATAELGAFFMVSMCLMAHPLKLDL+AGSFKALSRAACNIVLG IAFPPIAFFLFKG
Sbjct: 606 VINFATAELGAFFMVSMCLMAHPLKLDLVAGSFKALSRAACNIVLGFIAFPPIAFFLFKG 665

Query: 661 SLQGFHNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHP 692
           SLQGFH+LHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHP
Sbjct: 666 SLQGFHDLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHP 713

BLAST of MC08g0642 vs. NCBI nr
Match: XP_022144229.1 (LOW QUALITY PROTEIN: glycosylphosphatidylinositol anchor attachment 1 protein-like [Momordica charantia])

HSP 1 Score: 1243 bits (3217), Expect = 0.0
Identity = 656/709 (92.52%), Postives = 662/709 (93.37%), Query Frame = 0

Query: 1   TPKTKARPILRLGIFLISHSVFFSVFA-SPRVSALLLLPMFAMNTYISENALMPGSANTM 60
           TPKTKARPI+RLGIFLIS SVFFSV   S    ALLLLPMFAMNTYISENALMPGSANTM
Sbjct: 6   TPKTKARPIVRLGIFLISQSVFFSVICFSTGFXALLLLPMFAMNTYISENALMPGSANTM 65

Query: 61  LSGQEVSEANNLVKDLKALNSKPGSTVFGIQQILAQYISDLGAEVNYHKFHPQSGQFHPL 120
           LSGQEVSEANNLVKDLKALNSKPGSTVFGIQQILAQYISDLGAEVNYHKFHPQSGQFHPL
Sbjct: 66  LSGQEVSEANNLVKDLKALNSKPGSTVFGIQQILAQYISDLGAEVNYHKFHPQSGQFHPL 125

Query: 121 HFFSSPDSGIIRDNVSCMA-GINTVGIIRAPQADGKEAIVLVTTYNPVKTSLYETLSLGI 180
           HFFSSPDSGIIRDNVSCMA GINTVGIIRAPQADGKEAIVLVTTYNPVKTSLYETLSLGI
Sbjct: 126 HFFSSPDSGIIRDNVSCMAXGINTVGIIRAPQADGKEAIVLVTTYNPVKTSLYETLSLGI 185

Query: 181 AYSVFSLLTXVTWLAKDIVWLIVDSQYGEYAAVSAWLRDYHTPAF--------------C 240
           AYSVFSLLTXVTWLAKDIVWLIVDSQYGEYAAVSAWLRDY+TPAF               
Sbjct: 186 AYSVFSLLTXVTWLAKDIVWLIVDSQYGEYAAVSAWLRDYYTPAFGRSGIIDKDACAEMS 245

Query: 241 VLNKLETNHMTEKMSLDDFKRAGTMAAALVIKVSNKSERFEDSLSVYAEASNGQMPNLDL 300
           VLNKL TNHMTEKMSLD+FKRAGTMAAALVIKVSNKSERFEDSLSVYAEASNGQMPNLDL
Sbjct: 246 VLNKLGTNHMTEKMSLDEFKRAGTMAAALVIKVSNKSERFEDSLSVYAEASNGQMPNLDL 305

Query: 301 IN-VNYMAVHRQGFWIKVEKFWPLLDCKWLKVLGEVFESIGQLIRSLNSEWKFGILASDY 360
           IN VNYMAVHRQGFWIKVEKF PLLDCKWLKVLGEVFESIGQLIRSLNSEWKFGILASDY
Sbjct: 306 INXVNYMAVHRQGFWIKVEKFLPLLDCKWLKVLGEVFESIGQLIRSLNSEWKFGILASDY 365

Query: 361 VDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITMEMSPKFSSGIKVRHDEFYCEVAGQ 420
           VDG ATLASSLYYQAVGIPTGPHGAFRDYQVDAITMEMSPKFSSGIKVR DEF     G+
Sbjct: 366 VDGIATLASSLYYQAVGIPTGPHGAFRDYQVDAITMEMSPKFSSGIKVRRDEFXLR-GGR 425

Query: 421 LIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYAN 480
           LIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYAN
Sbjct: 426 LIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYAN 485

Query: 481 NLNLTVEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHVWGAVVSLLPYFICHLPGY 540
           NLNLTVEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVH+WGAVVSLLPYFIC +PGY
Sbjct: 486 NLNLTVEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHLWGAVVSLLPYFICQIPGY 545

Query: 541 SPTTSSTIWALLSLLPLLISSVILGLPFTCTKPPHQLIQECAFLKAMTISAAFIGLCLMS 600
           SPTTS TIWALLSLL LLI SVILG PFTCTKP HQLIQECAFLKAMTISAAFIGLCLMS
Sbjct: 546 SPTTSPTIWALLSLLTLLILSVILGSPFTCTKPSHQLIQECAFLKAMTISAAFIGLCLMS 605

Query: 601 VINFATAELGAFFMVSMCLMAHPLKLDLMAGSFKALSRAACNIVLGSIAFPPIAFFLFKG 660
           VINFATAELGAFFMVSMCLMAHPLKLDLMAGSFKALSRAACNIVLGSIAFPPIAFFLFKG
Sbjct: 606 VINFATAELGAFFMVSMCLMAHPLKLDLMAGSFKALSRAACNIVLGSIAFPPIAFFLFKG 665

Query: 661 SLQGFHNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHP 692
           SLQ       GDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHP
Sbjct: 666 SLQVSTIYIXGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHP 713

BLAST of MC08g0642 vs. NCBI nr
Match: XP_023550991.1 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1146 bits (2964), Expect = 0.0
Identity = 595/708 (84.04%), Postives = 635/708 (89.69%), Query Frame = 0

Query: 2   PKTKARPILRLGIFLISHSVFFSVFA-SPRVSALLLLPMFAMNTYISENALMPGSANTML 61
           PK+KARPI+RLGIFLISHS+FFSV   S  V ALLLLPMFAMNTYISENALMPGSANTML
Sbjct: 7   PKSKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPGSANTML 66

Query: 62  SGQEVSEANNLVKDLKALNSKPGSTVFGIQQILAQYISDLGAEVNYHKFHPQSGQFHPLH 121
           SGQEVSEAN+LVKD+K LNSK GST+FG QQILAQYIS+LGAEVNYH+F PQS QFHPLH
Sbjct: 67  SGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSSQFHPLH 126

Query: 122 FFSSPDSGIIRDNVSCMA-GINTVGIIRAPQADGKEAIVLVTTYNPVKTSLYETLSLGIA 181
           FFSSPDSGI++DNVSCM+ GINTVGIIRAPQADGKEAIVLVT YNPVKTSL+ETLSLGIA
Sbjct: 127 FFSSPDSGIVQDNVSCMSQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHETLSLGIA 186

Query: 182 YSVFSLLTXVTWLAKDIVWLIVDSQYGEYAAVSAWLRDYHTPAF----------C----V 241
           YS+FSLLT VTWLAKDIVWL  DS+YGEYAAVSAWLRDYHTP+F          C    V
Sbjct: 187 YSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYTCAETNV 246

Query: 242 LNKLETNHMTEKMSLDDFKRAGTMAAALVIKVSNKSERFEDSLSVYAEASNGQMPNLDLI 301
           L + E NH+TEK SLDDFKRAGTMAAALVIKVSN+SE FEDSLSVYAEASNGQMPNLDLI
Sbjct: 247 LYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLI 306

Query: 302 N-VNYMAVHRQGFWIKVEKFWPLLDCKWLKVLGEVFESIGQLIRSLNSEWKFGILASDYV 361
           N VNY+A HRQGF IK+EKFWPLLDCKWLK LGE FESIG+ IRSLN EWKFG+LASDYV
Sbjct: 307 NIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGMLASDYV 366

Query: 362 DGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITMEMSPKFSSGIKVRHDEFYCEVAGQL 421
           DGTATLASSLYYQAVGIPTGPHGAFRDYQ+DAIT+EMSPKFSSG+KVR DEF     G+L
Sbjct: 367 DGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILR-GGRL 426

Query: 422 IEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANN 481
           IEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANN
Sbjct: 427 IEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANN 486

Query: 482 LNLTVEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHVWGAVVSLLPYFICHLPGYS 541
           LNLT+EK+E A PANPDD LIVSLRSWKWLNAAK VFVVH+WGAVVSLLPYFIC +PGYS
Sbjct: 487 LNLTLEKIEPAAPANPDDVLIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQIPGYS 546

Query: 542 PTTSSTIWALLSLLPLLISSVILGLPFTCTKPPHQLIQECAFLKAMTISAAFIGLCLMSV 601
           PTT+S IWALLSLL LLI SVILG P + TK   Q IQE AFLKAMT SAAFIGLCLMSV
Sbjct: 547 PTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGLCLMSV 606

Query: 602 INFATAELGAFFMVSMCLMAHPLKLDLMAGSFKALSRAACNIVLGSIAFPPIAFFLFKGS 661
           INFATAELGAF +VSMCL+AHPLKLDL+AG+FKALSRAA N+VLG IAFPP+ FFLFK +
Sbjct: 607 INFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFFLFKAA 666

Query: 662 LQGFHNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHP 692
           LQGF NLH+GDFWNWMETLWAWNSATFLYLGMVHLPCW LC QILLHP
Sbjct: 667 LQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHP 713

BLAST of MC08g0642 vs. NCBI nr
Match: XP_038891214.1 (glycosylphosphatidylinositol anchor attachment 1 protein isoform X1 [Benincasa hispida])

HSP 1 Score: 1142 bits (2953), Expect = 0.0
Identity = 593/708 (83.76%), Postives = 630/708 (88.98%), Query Frame = 0

Query: 2   PKTKARPILRLGIFLISHSVFFSVFA-SPRVSALLLLPMFAMNTYISENALMPGSANTML 61
           PKTKARPI+RLGIFLISHS+FFSV   S  V ALLLLPMF+MNTYISENALMPGSANTML
Sbjct: 7   PKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFSMNTYISENALMPGSANTML 66

Query: 62  SGQEVSEANNLVKDLKALNSKPGSTVFGIQQILAQYISDLGAEVNYHKFHPQSGQFHPLH 121
           SG EVSEANNLVKDLK LNSKPGS +FG QQILAQYIS LGA+VNYH+FHPQS QFHPLH
Sbjct: 67  SGWEVSEANNLVKDLKGLNSKPGSAIFGSQQILAQYISKLGADVNYHRFHPQSSQFHPLH 126

Query: 122 FFSSPDSGIIRDNVSCMA-GINTVGIIRAPQADGKEAIVLVTTYNPVKTSLYETLSLGIA 181
           FFSSPDSGI+R NVSCM  GINTVGIIRAPQADGKEAIVLVT YNPVKTSL++TLSLGIA
Sbjct: 127 FFSSPDSGIVRGNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHDTLSLGIA 186

Query: 182 YSVFSLLTXVTWLAKDIVWLIVDSQYGEYAAVSAWLRDYHTPAF----------C----V 241
           YS+FSLLT VTWLAKDIVWL+ DS+YGEY AVSAWLRDYH P F          C    V
Sbjct: 187 YSIFSLLTQVTWLAKDIVWLVADSRYGEYDAVSAWLRDYHAPVFGRSSVIDTDACTETNV 246

Query: 242 LNKLETNHMTEKMSLDDFKRAGTMAAALVIKVSNKSERFEDSLSVYAEASNGQMPNLDLI 301
           L ++E NH TEK++LDDFKRAGTMAAALVIKVSN+SE FEDSLSVYAEASNGQMPNLDLI
Sbjct: 247 LYEVEANHATEKINLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLI 306

Query: 302 N-VNYMAVHRQGFWIKVEKFWPLLDCKWLKVLGEVFESIGQLIRSLNSEWKFGILASDYV 361
           N VNY+AVHRQGF IK+EKFWPLLDCKWLKVLGEVFESIG++IRSLNSEWKFG+ ASDYV
Sbjct: 307 NIVNYLAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGKIIRSLNSEWKFGMPASDYV 366

Query: 362 DGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITMEMSPKFSSGIKVRHDEFYCEVAGQL 421
           DGTATLASSLYYQAVGIPTG HGAFRDYQ+DAIT+EMSPKFSS +KVR DEF     G+L
Sbjct: 367 DGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSAVKVRRDEFILR-GGRL 426

Query: 422 IEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANN 481
           IEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAA+LYSYANN
Sbjct: 427 IEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAAALYSYANN 486

Query: 482 LNLTVEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHVWGAVVSLLPYFICHLPGYS 541
           +NLT+EKVE   PANPD+ELIVSLRSWKWLNAAK VFVVH+WGAVVSLLPYFIC +PGYS
Sbjct: 487 MNLTLEKVEPPAPANPDNELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQIPGYS 546

Query: 542 PTTSSTIWALLSLLPLLISSVILGLPFTCTKPPHQLIQECAFLKAMTISAAFIGLCLMSV 601
           PT +S IWALLSLL LLI SVILG P   TK   Q IQE AFLKAMT S AFI LCLMSV
Sbjct: 547 PTANSIIWALLSLLALLILSVILGSPLGSTKSYEQRIQEWAFLKAMTTSTAFISLCLMSV 606

Query: 602 INFATAELGAFFMVSMCLMAHPLKLDLMAGSFKALSRAACNIVLGSIAFPPIAFFLFKGS 661
           INFATAELGAF +VSMCL+AHPLKLDLMAGSFKALSRAACNIVLG IAFPP+ FFLFK +
Sbjct: 607 INFATAELGAFLVVSMCLLAHPLKLDLMAGSFKALSRAACNIVLGFIAFPPVTFFLFKAA 666

Query: 662 LQGFHNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHP 692
           LQGF NLHIGDFWNWMETLWAWNSATFLYLGMVHLPCW LC QILLHP
Sbjct: 667 LQGFDNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHP 713

BLAST of MC08g0642 vs. NCBI nr
Match: KAG7016207.1 (Glycosylphosphatidylinositol anchor attachment 1 protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1141 bits (2952), Expect = 0.0
Identity = 590/708 (83.33%), Postives = 634/708 (89.55%), Query Frame = 0

Query: 2   PKTKARPILRLGIFLISHSVFFSVFA-SPRVSALLLLPMFAMNTYISENALMPGSANTML 61
           PKTKARPI+RLGIFLISHS+FFSV   S  + ALLLLPMFA+NTYISENALMPGSANTML
Sbjct: 7   PKTKARPIVRLGIFLISHSIFFSVICFSAGILALLLLPMFALNTYISENALMPGSANTML 66

Query: 62  SGQEVSEANNLVKDLKALNSKPGSTVFGIQQILAQYISDLGAEVNYHKFHPQSGQFHPLH 121
           SGQEVSEAN+LVKD+K LNSK GST+FG QQILAQYIS+LGAEVNYH+F PQS QFHPLH
Sbjct: 67  SGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSSQFHPLH 126

Query: 122 FFSSPDSGIIRDNVSCMA-GINTVGIIRAPQADGKEAIVLVTTYNPVKTSLYETLSLGIA 181
           FFSSPDSGI++DNVSCM  GINTVGIIRAPQADGKEAIVLVT YNPVKTSL+ETLSLGIA
Sbjct: 127 FFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHETLSLGIA 186

Query: 182 YSVFSLLTXVTWLAKDIVWLIVDSQYGEYAAVSAWLRDYHTPAF----------C----V 241
           YS+FSLLT VTWLAKDIVWL  DS+YGEYAAVSAWLRDYHTP+F          C    V
Sbjct: 187 YSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYTCAETNV 246

Query: 242 LNKLETNHMTEKMSLDDFKRAGTMAAALVIKVSNKSERFEDSLSVYAEASNGQMPNLDLI 301
           L + E NH+TEK SLDDFKRAGTMAAAL+IKVSN+SE FEDSLSVYAEASNGQMPNLDLI
Sbjct: 247 LYEFEANHVTEKESLDDFKRAGTMAAALIIKVSNRSEHFEDSLSVYAEASNGQMPNLDLI 306

Query: 302 N-VNYMAVHRQGFWIKVEKFWPLLDCKWLKVLGEVFESIGQLIRSLNSEWKFGILASDYV 361
           N VNY+A HRQGF IK+EKFWPLLDCKWLK LGE+FE+IG+ IRSLN EWKFG+LASDYV
Sbjct: 307 NIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEIFETIGKFIRSLNPEWKFGMLASDYV 366

Query: 362 DGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITMEMSPKFSSGIKVRHDEFYCEVAGQL 421
           DGTATLASSLYYQAVGIPTGPHGAFRDYQ+DAIT+EMSPKFSSG+KVR DEF     G+L
Sbjct: 367 DGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILR-GGRL 426

Query: 422 IEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANN 481
           IEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANN
Sbjct: 427 IEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANN 486

Query: 482 LNLTVEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHVWGAVVSLLPYFICHLPGYS 541
           LNLT+EK+E A PANPDDELIVSLRSW+WLNAAK VFVVH+WGAVVSLLPYFIC +P YS
Sbjct: 487 LNLTLEKIEPAAPANPDDELIVSLRSWRWLNAAKRVFVVHLWGAVVSLLPYFICQIPDYS 546

Query: 542 PTTSSTIWALLSLLPLLISSVILGLPFTCTKPPHQLIQECAFLKAMTISAAFIGLCLMSV 601
           PTT+S IWALLSLL LLI SVILG P + TK   Q IQE AFLKAMT SAAFIGLCLMSV
Sbjct: 547 PTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGLCLMSV 606

Query: 602 INFATAELGAFFMVSMCLMAHPLKLDLMAGSFKALSRAACNIVLGSIAFPPIAFFLFKGS 661
           INFATAELGAF +VSMCL+AHPLKLDL+A +FKALSRAA N+VLG IAFPP+ FFLFK +
Sbjct: 607 INFATAELGAFLVVSMCLLAHPLKLDLVAWNFKALSRAASNLVLGFIAFPPVTFFLFKAA 666

Query: 662 LQGFHNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHP 692
           LQGF NLH+GDFWNWMETLWAWNSATFLYLGMVHLPCW LC QILLHP
Sbjct: 667 LQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHP 713

BLAST of MC08g0642 vs. ExPASy TrEMBL
Match: A0A6J1BZU7 (LOW QUALITY PROTEIN: glycosylphosphatidylinositol anchor attachment 1 protein-like OS=Momordica charantia OX=3673 GN=LOC111006214 PE=4 SV=1)

HSP 1 Score: 1246 bits (3223), Expect = 0.0
Identity = 652/709 (91.96%), Postives = 662/709 (93.37%), Query Frame = 0

Query: 1   TPKTKARPILRLGIFLISHSVFFSVFA-SPRVSALLLLPMFAMNTYISENALMPGSANTM 60
           TPK KARPI+RLGIFLISHSVFFSV   S  V ALLLLPMFAMNTYISENALMPGSANTM
Sbjct: 6   TPKAKARPIVRLGIFLISHSVFFSVICFSAGVLALLLLPMFAMNTYISENALMPGSANTM 65

Query: 61  LSGQEVSEANNLVKDLKALNSKPGSTVFGIQQILAQYISDLGAEVNYHKFHPQSGQFHPL 120
           LSGQEVSEANNLVKD+KALNSKPGSTVFG QQILAQYISDLGAEVNYHKFHPQSGQFHPL
Sbjct: 66  LSGQEVSEANNLVKDIKALNSKPGSTVFGSQQILAQYISDLGAEVNYHKFHPQSGQFHPL 125

Query: 121 HFFSSPDSGIIRDNVSCMA-GINTVGIIRAPQADGKEAIVLVTTYNPVKTSLYETLSLGI 180
           HFFSSPDSGIIRDNVSCMA GINTVGII+APQADGKEAIVLVTTYNPVKTS YETLSLGI
Sbjct: 126 HFFSSPDSGIIRDNVSCMAQGINTVGIIKAPQADGKEAIVLVTTYNPVKTSXYETLSLGI 185

Query: 181 AYSVFSLLTXVTWLAKDIVWLIVDSQYGEYAAVSAWLRDYHTPAF--------------C 240
           AYSVFSLLTXVTWLAKDIVWLIVDSQYGEYAAVSAWLRDY+TPAF               
Sbjct: 186 AYSVFSLLTXVTWLAKDIVWLIVDSQYGEYAAVSAWLRDYYTPAFGRSGIIDKDACAEMS 245

Query: 241 VLNKLETNHMTEKMSLDDFKRAGTMAAALVIKVSNKSERFEDSLSVYAEASNGQMPNLDL 300
           VLNKL TNHMTEKMSLD+FKRAGTMAAALVIKVSNKSE FEDSLSVYAEASNGQMPNLDL
Sbjct: 246 VLNKLGTNHMTEKMSLDEFKRAGTMAAALVIKVSNKSEHFEDSLSVYAEASNGQMPNLDL 305

Query: 301 IN-VNYMAVHRQGFWIKVEKFWPLLDCKWLKVLGEVFESIGQLIRSLNSEWKFGILASDY 360
           IN VNYMAVHRQGF IK+EKFWPLLDCKWLKVLGEVFESIGQLIR LN EWKFGI ASDY
Sbjct: 306 INIVNYMAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGQLIRILNPEWKFGIPASDY 365

Query: 361 VDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITMEMSPKFSSGIKVRHDEFYCEVAGQ 420
           VDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAIT+EMSPKFSSGIKVR DEF     G+
Sbjct: 366 VDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITVEMSPKFSSGIKVRRDEFILR-GGR 425

Query: 421 LIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYAN 480
           LIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYAN
Sbjct: 426 LIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYAN 485

Query: 481 NLNLTVEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHVWGAVVSLLPYFICHLPGY 540
           NLNLT EKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVH WGAVVSLLPYFIC LPGY
Sbjct: 486 NLNLTXEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHXWGAVVSLLPYFICXLPGY 545

Query: 541 SPTTSSTIWALLSLLPLLISSVILGLPFTCTKPPHQLIQECAFLKAMTISAAFIGLCLMS 600
           SPTTS TIWALLSLL LLI SVILG PFTCTKP HQLIQECAFLKAMTISAAFIGLCLMS
Sbjct: 546 SPTTSXTIWALLSLLXLLIXSVILGXPFTCTKPXHQLIQECAFLKAMTISAAFIGLCLMS 605

Query: 601 VINFATAELGAFFMVSMCLMAHPLKLDLMAGSFKALSRAACNIVLGSIAFPPIAFFLFKG 660
           VINFATAELGAFFMVSMCLMAHPLKLDL+AGSFKALSRAACNIVLG IAFPPIAFFLFKG
Sbjct: 606 VINFATAELGAFFMVSMCLMAHPLKLDLVAGSFKALSRAACNIVLGFIAFPPIAFFLFKG 665

Query: 661 SLQGFHNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHP 692
           SLQGFH+LHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHP
Sbjct: 666 SLQGFHDLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHP 713

BLAST of MC08g0642 vs. ExPASy TrEMBL
Match: A0A6J1CRQ0 (LOW QUALITY PROTEIN: glycosylphosphatidylinositol anchor attachment 1 protein-like OS=Momordica charantia OX=3673 GN=LOC111013966 PE=4 SV=1)

HSP 1 Score: 1243 bits (3217), Expect = 0.0
Identity = 656/709 (92.52%), Postives = 662/709 (93.37%), Query Frame = 0

Query: 1   TPKTKARPILRLGIFLISHSVFFSVFA-SPRVSALLLLPMFAMNTYISENALMPGSANTM 60
           TPKTKARPI+RLGIFLIS SVFFSV   S    ALLLLPMFAMNTYISENALMPGSANTM
Sbjct: 6   TPKTKARPIVRLGIFLISQSVFFSVICFSTGFXALLLLPMFAMNTYISENALMPGSANTM 65

Query: 61  LSGQEVSEANNLVKDLKALNSKPGSTVFGIQQILAQYISDLGAEVNYHKFHPQSGQFHPL 120
           LSGQEVSEANNLVKDLKALNSKPGSTVFGIQQILAQYISDLGAEVNYHKFHPQSGQFHPL
Sbjct: 66  LSGQEVSEANNLVKDLKALNSKPGSTVFGIQQILAQYISDLGAEVNYHKFHPQSGQFHPL 125

Query: 121 HFFSSPDSGIIRDNVSCMA-GINTVGIIRAPQADGKEAIVLVTTYNPVKTSLYETLSLGI 180
           HFFSSPDSGIIRDNVSCMA GINTVGIIRAPQADGKEAIVLVTTYNPVKTSLYETLSLGI
Sbjct: 126 HFFSSPDSGIIRDNVSCMAXGINTVGIIRAPQADGKEAIVLVTTYNPVKTSLYETLSLGI 185

Query: 181 AYSVFSLLTXVTWLAKDIVWLIVDSQYGEYAAVSAWLRDYHTPAF--------------C 240
           AYSVFSLLTXVTWLAKDIVWLIVDSQYGEYAAVSAWLRDY+TPAF               
Sbjct: 186 AYSVFSLLTXVTWLAKDIVWLIVDSQYGEYAAVSAWLRDYYTPAFGRSGIIDKDACAEMS 245

Query: 241 VLNKLETNHMTEKMSLDDFKRAGTMAAALVIKVSNKSERFEDSLSVYAEASNGQMPNLDL 300
           VLNKL TNHMTEKMSLD+FKRAGTMAAALVIKVSNKSERFEDSLSVYAEASNGQMPNLDL
Sbjct: 246 VLNKLGTNHMTEKMSLDEFKRAGTMAAALVIKVSNKSERFEDSLSVYAEASNGQMPNLDL 305

Query: 301 IN-VNYMAVHRQGFWIKVEKFWPLLDCKWLKVLGEVFESIGQLIRSLNSEWKFGILASDY 360
           IN VNYMAVHRQGFWIKVEKF PLLDCKWLKVLGEVFESIGQLIRSLNSEWKFGILASDY
Sbjct: 306 INXVNYMAVHRQGFWIKVEKFLPLLDCKWLKVLGEVFESIGQLIRSLNSEWKFGILASDY 365

Query: 361 VDGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITMEMSPKFSSGIKVRHDEFYCEVAGQ 420
           VDG ATLASSLYYQAVGIPTGPHGAFRDYQVDAITMEMSPKFSSGIKVR DEF     G+
Sbjct: 366 VDGIATLASSLYYQAVGIPTGPHGAFRDYQVDAITMEMSPKFSSGIKVRRDEFXLR-GGR 425

Query: 421 LIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYAN 480
           LIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYAN
Sbjct: 426 LIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYAN 485

Query: 481 NLNLTVEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHVWGAVVSLLPYFICHLPGY 540
           NLNLTVEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVH+WGAVVSLLPYFIC +PGY
Sbjct: 486 NLNLTVEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHLWGAVVSLLPYFICQIPGY 545

Query: 541 SPTTSSTIWALLSLLPLLISSVILGLPFTCTKPPHQLIQECAFLKAMTISAAFIGLCLMS 600
           SPTTS TIWALLSLL LLI SVILG PFTCTKP HQLIQECAFLKAMTISAAFIGLCLMS
Sbjct: 546 SPTTSPTIWALLSLLTLLILSVILGSPFTCTKPSHQLIQECAFLKAMTISAAFIGLCLMS 605

Query: 601 VINFATAELGAFFMVSMCLMAHPLKLDLMAGSFKALSRAACNIVLGSIAFPPIAFFLFKG 660
           VINFATAELGAFFMVSMCLMAHPLKLDLMAGSFKALSRAACNIVLGSIAFPPIAFFLFKG
Sbjct: 606 VINFATAELGAFFMVSMCLMAHPLKLDLMAGSFKALSRAACNIVLGSIAFPPIAFFLFKG 665

Query: 661 SLQGFHNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHP 692
           SLQ       GDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHP
Sbjct: 666 SLQVSTIYIXGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHP 713

BLAST of MC08g0642 vs. ExPASy TrEMBL
Match: A0A6J1FHQ4 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445544 PE=4 SV=1)

HSP 1 Score: 1139 bits (2946), Expect = 0.0
Identity = 590/708 (83.33%), Postives = 634/708 (89.55%), Query Frame = 0

Query: 2   PKTKARPILRLGIFLISHSVFFSVFA-SPRVSALLLLPMFAMNTYISENALMPGSANTML 61
           PK+KARPI+RLGIFLISHS+FFSV   S  V ALLLLPMFA+NTYISENALMPGSANTML
Sbjct: 7   PKSKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAINTYISENALMPGSANTML 66

Query: 62  SGQEVSEANNLVKDLKALNSKPGSTVFGIQQILAQYISDLGAEVNYHKFHPQSGQFHPLH 121
           SGQEVSEAN+LVKD+K LNSK GST+FG QQILAQYIS+LGAEVNYH+F PQS +FHPLH
Sbjct: 67  SGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSSRFHPLH 126

Query: 122 FFSSPDSGIIRDNVSCMA-GINTVGIIRAPQADGKEAIVLVTTYNPVKTSLYETLSLGIA 181
           FFSSPDSGI++DNVSCM  GINTVGIIRAPQADGKEAIVLVT YNPVKTSL+ETLSLGIA
Sbjct: 127 FFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHETLSLGIA 186

Query: 182 YSVFSLLTXVTWLAKDIVWLIVDSQYGEYAAVSAWLRDYHTPAF----------C----V 241
           YS+FSLLT VTWLAKDIVWL  DS+YGEYAAVSAWLRDYHTP+F          C    V
Sbjct: 187 YSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYTCAETNV 246

Query: 242 LNKLETNHMTEKMSLDDFKRAGTMAAALVIKVSNKSERFEDSLSVYAEASNGQMPNLDLI 301
           L + E NH+TEK SLDDFKRAGTMAAALVIKVSN+SE FEDSLSVYAEASNGQMPNLDLI
Sbjct: 247 LYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLI 306

Query: 302 N-VNYMAVHRQGFWIKVEKFWPLLDCKWLKVLGEVFESIGQLIRSLNSEWKFGILASDYV 361
           N VNY+A HRQGF IK+EKFWPLLDCKWLK LGE+FE+IG+ IRSLN EWKFG+LASDYV
Sbjct: 307 NIVNYLAEHRQGFQIKIEKFWPLLDCKWLKFLGEIFETIGKFIRSLNPEWKFGMLASDYV 366

Query: 362 DGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITMEMSPKFSSGIKVRHDEFYCEVAGQL 421
           DGTATLASSLYYQAVGIPTGPHGAFRDYQ+DAIT+EMSPKFSSG+KVR DEF     G+L
Sbjct: 367 DGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILR-GGRL 426

Query: 422 IEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANN 481
           IEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANN
Sbjct: 427 IEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANN 486

Query: 482 LNLTVEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHVWGAVVSLLPYFICHLPGYS 541
            NLT+EK+E A PANPDDELIVSLRSW+WLNAAK VFVVH+WGAVVSLLPYFIC +P YS
Sbjct: 487 SNLTLEKIEPAAPANPDDELIVSLRSWRWLNAAKRVFVVHLWGAVVSLLPYFICPIPDYS 546

Query: 542 PTTSSTIWALLSLLPLLISSVILGLPFTCTKPPHQLIQECAFLKAMTISAAFIGLCLMSV 601
           PTT+S IWALLSLL LLI SVILG P + TK   Q IQE AFLKAMT SAAFIGLCLMSV
Sbjct: 547 PTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGLCLMSV 606

Query: 602 INFATAELGAFFMVSMCLMAHPLKLDLMAGSFKALSRAACNIVLGSIAFPPIAFFLFKGS 661
           INFATAELGAF +VSMCL+AHPLKLDL+AG+FKALSRAA N+VLG IAFPP+ FFLFK +
Sbjct: 607 INFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFFLFKAA 666

Query: 662 LQGFHNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHP 692
           LQGF NLH+GDFWNWMETLWAWNSATFLYLGMVHLPCW LC QILLHP
Sbjct: 667 LQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHP 713

BLAST of MC08g0642 vs. ExPASy TrEMBL
Match: A0A6J1K1W5 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489371 PE=4 SV=1)

HSP 1 Score: 1137 bits (2940), Expect = 0.0
Identity = 593/708 (83.76%), Postives = 631/708 (89.12%), Query Frame = 0

Query: 2   PKTKARPILRLGIFLISHSVFFSVFA-SPRVSALLLLPMFAMNTYISENALMPGSANTML 61
           PKTKARPI+RLGIFLISHS+FFSV   S  V ALLLLPMFAMNTYISENALMPGSANTML
Sbjct: 7   PKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPGSANTML 66

Query: 62  SGQEVSEANNLVKDLKALNSKPGSTVFGIQQILAQYISDLGAEVNYHKFHPQSGQFHPLH 121
           SGQEVSEAN+LVKDLK LNSK GST+FG QQILAQYIS+LGAEVNYH+F PQS QFHPLH
Sbjct: 67  SGQEVSEANDLVKDLKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSSQFHPLH 126

Query: 122 FFSSPDSGIIRDNVSCMA-GINTVGIIRAPQADGKEAIVLVTTYNPVKTSLYETLSLGIA 181
           FFSSPDSGI++DNVSCM  GINTVGIIRAPQADGKEAIVLVT YNPVKTSL+ETLSLGIA
Sbjct: 127 FFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHETLSLGIA 186

Query: 182 YSVFSLLTXVTWLAKDIVWLIVDSQYGEYAAVSAWLRDYHTPAF----------C----V 241
           YS+FSLLT V+WLAKDIVWL  DS+YGEYAAVSAWLRDYHTP+F          C    V
Sbjct: 187 YSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYTCAETNV 246

Query: 242 LNKLETNHMTEKMSLDDFKRAGTMAAALVIKVSNKSERFEDSLSVYAEASNGQMPNLDLI 301
           L + E NH+TEK SLDDFKRAGTMAAALVIKVSN+SE FEDSLSVYAEASNGQMPNLDLI
Sbjct: 247 LYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLI 306

Query: 302 N-VNYMAVHRQGFWIKVEKFWPLLDCKWLKVLGEVFESIGQLIRSLNSEWKFGILASDYV 361
           N VNY+A HRQGF IK+EKFWPLLDCKWLK LGE FESIG+ IRSLN EWKFG+LASDYV
Sbjct: 307 NIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGMLASDYV 366

Query: 362 DGTATLASSLYYQAVGIPTGPHGAFRDYQVDAITMEMSPKFSSGIKVRHDEFYCEVAGQL 421
           DGTATLASSLYYQAVGIPTG HGAFRDYQ+DAIT+EMSPKFSSG+KVR DEF     G+L
Sbjct: 367 DGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILR-GGRL 426

Query: 422 IEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANN 481
           IEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANN
Sbjct: 427 IEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANN 486

Query: 482 LNLTVEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHVWGAVVSLLPYFICHLPGYS 541
            NLT+EK+E A PAN DDELIVSLRSWKWLNAAK VFVVH+WGAVVSLLPYFI  +PGYS
Sbjct: 487 SNLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFISQIPGYS 546

Query: 542 PTTSSTIWALLSLLPLLISSVILGLPFTCTKPPHQLIQECAFLKAMTISAAFIGLCLMSV 601
           PTT+S IWALLSLL LLI SVILG P + TK   Q IQE AFLKAMT SAAFIGLCLMSV
Sbjct: 547 PTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQEWAFLKAMTTSAAFIGLCLMSV 606

Query: 602 INFATAELGAFFMVSMCLMAHPLKLDLMAGSFKALSRAACNIVLGSIAFPPIAFFLFKGS 661
           INFATAELGAF +VSMCL+AHPLKLDL+AG+FKALSRAA N+VLG IAFPP+ FFLFK +
Sbjct: 607 INFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFFLFKAA 666

Query: 662 LQGFHNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHP 692
           LQGF NLH+GDFWNWMETLWAWNSATFLYLGMVHLPCW LC QILLHP
Sbjct: 667 LQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHP 713

BLAST of MC08g0642 vs. ExPASy TrEMBL
Match: A0A0A0KP05 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G523060 PE=4 SV=1)

HSP 1 Score: 1134 bits (2933), Expect = 0.0
Identity = 588/707 (83.17%), Postives = 632/707 (89.39%), Query Frame = 0

Query: 3   KTKARPILRLGIFLISHSVFFSVFA-SPRVSALLLLPMFAMNTYISENALMPGSANTMLS 62
           K KARPI+RLGIFLISHS+FFSV   S  V ALLLLPMFAMNTYISENALMPGSAN+MLS
Sbjct: 8   KPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPGSANSMLS 67

Query: 63  GQEVSEANNLVKDLKALNSKPGSTVFGIQQILAQYISDLGAEVNYHKFHPQSGQFHPLHF 122
           G+EVSEANNLVKDLK LNSKPGS++FG QQILAQYIS LGA+VNYH+FHPQS QFHPLHF
Sbjct: 68  GREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSSQFHPLHF 127

Query: 123 FSSPDSGIIRDNVSCMA-GINTVGIIRAPQADGKEAIVLVTTYNPVKTSLYETLSLGIAY 182
           FSSPDSGI++DNVSCM  GINTVGIIRAPQADGKEAIVLVT YNPV+TSL++TLSLGIAY
Sbjct: 128 FSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDTLSLGIAY 187

Query: 183 SVFSLLTXVTWLAKDIVWLIVDSQYGEYAAVSAWLRDYHTPAF----------C----VL 242
           S+FSLLT VTWLAKDIVWL+ DS+YGEYAAVSAWLRDYHTP F          C    VL
Sbjct: 188 SIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSSVIDTDACSETNVL 247

Query: 243 NKLETNHMTEKMSLDDFKRAGTMAAALVIKVSNKSERFEDSLSVYAEASNGQMPNLDLIN 302
           ++ E N +TEK  LDDFKRAGTMAAALVIKVSN+SE FEDSLSVYAEASNGQMPNLDLIN
Sbjct: 248 DEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLIN 307

Query: 303 -VNYMAVHRQGFWIKVEKFWPLLDCKWLKVLGEVFESIGQLIRSLNSEWKFGILASDYVD 362
            VNY+AVHRQGF IK+EKFWPLL+CKWLKVLGEVFESIG++IRSLNSEWKFG+ ASDYVD
Sbjct: 308 IVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGMSASDYVD 367

Query: 363 GTATLASSLYYQAVGIPTGPHGAFRDYQVDAITMEMSPKFSSGIKVRHDEFYCEVAGQLI 422
           GTATLASSLYYQAVGIPTG HGAFRDYQ+DAIT+EMSPKFSSGIKVR D+F     G+LI
Sbjct: 368 GTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDFILR-GGRLI 427

Query: 423 EGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNL 482
           EGVVRSVNNLLEKFHQSFFLYLMVST KFVSVGVYMIAFALLVAPLPAVAA+LYSYANNL
Sbjct: 428 EGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALYSYANNL 487

Query: 483 NLTVEKVEHAVPANPDDELIVSLRSWKWLNAAKNVFVVHVWGAVVSLLPYFICHLPGYSP 542
           NLT EKVE    ANPDDELIVSLRSWKWLNAAK VFVVH+WGA+VSLLPYFIC +PGYSP
Sbjct: 488 NLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFICQIPGYSP 547

Query: 543 TTSSTIWALLSLLPLLISSVILGLPFTCTKPPHQLIQECAFLKAMTISAAFIGLCLMSVI 602
           T +S IW LLSLL LL+ SVILG PF+ TK   Q IQE AFLKAMT SAAFIGLCLMSVI
Sbjct: 548 TENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMTTSAAFIGLCLMSVI 607

Query: 603 NFATAELGAFFMVSMCLMAHPLKLDLMAGSFKALSRAACNIVLGSIAFPPIAFFLFKGSL 662
           NF+TAELGAF +VSMCL+AHPLKLDL AG+FKALSRAACN+VLG IAFPP+ FFLFKG+L
Sbjct: 608 NFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFFLFKGAL 667

Query: 663 QGFHNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHP 692
           QGF NLHIGDFWNWMETLWAWNSATFLYLGMVHLPCW LC QILLHP
Sbjct: 668 QGFDNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWLLCTQILLHP 713

BLAST of MC08g0642 vs. TAIR 10
Match: AT5G19130.1 (GPI transamidase component family protein / Gaa1-like family protein )

HSP 1 Score: 758.4 bits (1957), Expect = 4.9e-219
Identity = 405/704 (57.53%), Postives = 520/704 (73.86%), Query Frame = 0

Query: 1   TPKTKARPILRLGIFLISHSVFFS-VFASPRVSALLLLPMFAMNTYISENALMPGSANTM 60
           +PK K RPI+RLGIFLI+HS  FS VF++  V ALLLLP+ A NTYISENALMPGSA +M
Sbjct: 18  SPKIKPRPIVRLGIFLIAHSPVFSVVFSAAGVLALLLLPLLAKNTYISENALMPGSARSM 77

Query: 61  LSGQEVSEANNLVKDLKALNSKPGSTVFGIQQILAQYISDLGAEVNYHKFHPQSGQFHPL 120
           LS ++VS+ + LVKD+K            +Q+++ +Y+SD+GAEV+Y KFHP+  QFHPL
Sbjct: 78  LSNRDVSDGSKLVKDIKNFRLNHEGQGVEVQKLIGKYMSDMGAEVSYQKFHPEGNQFHPL 137

Query: 121 HFFSSPDSGIIRDNVSCMA-GINTVGIIRAPQADGKEAIVLVTTYNPVKTSLYETLSLGI 180
           HFFS PDS  + +NVSC + G+N  GIIRAP+ DGKE+IVLVT Y+ +    YE LSLGI
Sbjct: 138 HFFSGPDSYTLLENVSCASYGVNVAGIIRAPRGDGKESIVLVTPYDFINGGDYEDLSLGI 197

Query: 181 AYSVFSLLTXVTWLAKDIVWLIVDSQYGEYAAVSAWLRDYHTPAFCVLNKLETNHMTEKM 240
             S+FSLL+ VTWL+KDI+WL+ DS+YG+Y  V+AWL +YH+P+F V + L+ +   E+ 
Sbjct: 198 VSSLFSLLSRVTWLSKDIIWLVADSRYGDYRPVAAWLTEYHSPSFKVSDLLKCD---EQN 257

Query: 241 SLDDFKRAGTMAAALVIKVSNKSERFEDSLSVYAEASNGQMPNLDLIN-VNYMAVHRQGF 300
           + D+F+RAGTMAAALV+KV  +SE+FED+LS+YAEASNGQMPNLDLIN VNY+AVHRQGF
Sbjct: 258 TADNFRRAGTMAAALVLKVDGRSEKFEDTLSIYAEASNGQMPNLDLINVVNYLAVHRQGF 317

Query: 301 WIKVEKFWPLLDCKWLKVLGEVFESIGQLIRSLNSEWKFGILASDYVDGTATLASSLYYQ 360
           ++KVEK   LL   WLK+ GE+FE++G+L   LN +W FGI A+DY++G+ATLASSLY Q
Sbjct: 318 YVKVEKVVSLLSSSWLKIFGEIFEAVGKLAHMLNPDWNFGIPAADYLEGSATLASSLYSQ 377

Query: 361 AVGIPTGPHGAFRDYQVDAITMEMSPKFSSGIKVRHDEFYCEVAGQLIEGVVRSVNNLLE 420
           A+GIPTGPHGAFRDYQVDAIT+++SP+F    K R  +F+   A +L+EG +RSVNNLLE
Sbjct: 378 ALGIPTGPHGAFRDYQVDAITLKVSPRFPPDSKTRQHDFFLRGA-RLLEGTIRSVNNLLE 437

Query: 421 KFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY-----SYANNLNLTVEKV 480
           KFHQSFFLY++ S SKF+SVGVYMIAFALLVAPLP VAASLY     S  N+ +      
Sbjct: 438 KFHQSFFLYMLTSPSKFISVGVYMIAFALLVAPLPMVAASLYIDGCKSLTNSTH------ 497

Query: 481 EHAVPANPDDELIVSLRSWKWLNAAKNVFVVHVWGAVVSLLPYFICHLPG-YSPTTSSTI 540
                 NP +    + +SWKWL+AAK VF +H+ G +V+LLPYFIC +PG +SPT  S +
Sbjct: 498 ------NPTE----NFKSWKWLDAAKQVFTLHLLGFIVTLLPYFICQVPGQHSPTNRSIM 557

Query: 541 WALLSLLPLLISSVILGLPFTCTKPPHQLI-QECAFLKAMTISAAFIGLCLMSVINFATA 600
           W   S   LLI+ V +     C+    +L     A LK++TISAAFIGLCLMS+INFATA
Sbjct: 558 WGTTSSSLLLITFVTMP---GCSPFSSRLHGTNWAILKSVTISAAFIGLCLMSIINFATA 617

Query: 601 ELGAFFMVSMCLMAHPLKLDLMAGSFKALSRAACNIVLGSIAFPPIAFFLFKGSL-QGFH 660
           E+GA  +V  CLMA P+K  L +   K+L  A C+IVL +I FP + F + KG L +G  
Sbjct: 618 EIGALLLVPTCLMAQPIKPALRSRRLKSLLGAFCSIVLLTIGFPVMFFAISKGLLGEGLV 677

Query: 661 NLHI-GDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHP 693
            L + G+FW W+E+LWAW SAT+LY+GMVHLPCW LC+ IL HP
Sbjct: 678 GLSLGGEFWTWLESLWAWKSATYLYIGMVHLPCWLLCLCILFHP 698

BLAST of MC08g0642 vs. TAIR 10
Match: AT5G19130.2 (GPI transamidase component family protein / Gaa1-like family protein )

HSP 1 Score: 755.7 bits (1950), Expect = 3.2e-218
Identity = 406/704 (57.67%), Postives = 520/704 (73.86%), Query Frame = 0

Query: 1   TPKTKARPILRLGIFLISHSVFFS-VFASPRVSALLLLPMFAMNTYISENALMPGSANTM 60
           +PK K RPI+RLGIFLI+HS  FS VF++  V ALLLLP+ A NTYISENALMPGSA +M
Sbjct: 18  SPKIKPRPIVRLGIFLIAHSPVFSVVFSAAGVLALLLLPLLAKNTYISENALMPGSARSM 77

Query: 61  LSGQEVSEANNLVKDLKALNSKPGSTVFGIQQILAQYISDLGAEVNYHKFHPQSGQFHPL 120
           LS ++VS+ + LVKD+K         V   Q+++ +Y+SD+GAEV+Y KFHP+  QFHPL
Sbjct: 78  LSNRDVSDGSKLVKDIKNFRLNHEGQV---QKLIGKYMSDMGAEVSYQKFHPEGNQFHPL 137

Query: 121 HFFSSPDSGIIRDNVSCMA-GINTVGIIRAPQADGKEAIVLVTTYNPVKTSLYETLSLGI 180
           HFFS PDS  + +NVSC + G+N  GIIRAP+ DGKE+IVLVT Y+ +    YE LSLGI
Sbjct: 138 HFFSGPDSYTLLENVSCASYGVNVAGIIRAPRGDGKESIVLVTPYDFINGGDYEDLSLGI 197

Query: 181 AYSVFSLLTXVTWLAKDIVWLIVDSQYGEYAAVSAWLRDYHTPAFCVLNKLETNHMTEKM 240
             S+FSLL+ VTWL+KDI+WL+ DS+YG+Y  V+AWL +YH+P+F V + L+ +   E+ 
Sbjct: 198 VSSLFSLLSRVTWLSKDIIWLVADSRYGDYRPVAAWLTEYHSPSFKVSDLLKCD---EQN 257

Query: 241 SLDDFKRAGTMAAALVIKVSNKSERFEDSLSVYAEASNGQMPNLDLIN-VNYMAVHRQGF 300
           + D+F+RAGTMAAALV+KV  +SE+FED+LS+YAEASNGQMPNLDLIN VNY+AVHRQGF
Sbjct: 258 TADNFRRAGTMAAALVLKVDGRSEKFEDTLSIYAEASNGQMPNLDLINVVNYLAVHRQGF 317

Query: 301 WIKVEKFWPLLDCKWLKVLGEVFESIGQLIRSLNSEWKFGILASDYVDGTATLASSLYYQ 360
           ++KVEK   LL   WLK+ GE+FE++G+L   LN +W FGI A+DY++G+ATLASSLY Q
Sbjct: 318 YVKVEKVVSLLSSSWLKIFGEIFEAVGKLAHMLNPDWNFGIPAADYLEGSATLASSLYSQ 377

Query: 361 AVGIPTGPHGAFRDYQVDAITMEMSPKFSSGIKVRHDEFYCEVAGQLIEGVVRSVNNLLE 420
           A+GIPTGPHGAFRDYQVDAIT+++SP+F    K R  +F+   A +L+EG +RSVNNLLE
Sbjct: 378 ALGIPTGPHGAFRDYQVDAITLKVSPRFPPDSKTRQHDFFLRGA-RLLEGTIRSVNNLLE 437

Query: 421 KFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY-----SYANNLNLTVEKV 480
           KFHQSFFLY++ S SKF+SVGVYMIAFALLVAPLP VAASLY     S  N+ +      
Sbjct: 438 KFHQSFFLYMLTSPSKFISVGVYMIAFALLVAPLPMVAASLYIDGCKSLTNSTH------ 497

Query: 481 EHAVPANPDDELIVSLRSWKWLNAAKNVFVVHVWGAVVSLLPYFICHLPG-YSPTTSSTI 540
                 NP +    + +SWKWL+AAK VF +H+ G +V+LLPYFIC +PG +SPT  S +
Sbjct: 498 ------NPTE----NFKSWKWLDAAKQVFTLHLLGFIVTLLPYFICQVPGQHSPTNRSIM 557

Query: 541 WALLSLLPLLISSVILGLPFTCTKPPHQLI-QECAFLKAMTISAAFIGLCLMSVINFATA 600
           W   S   LLI+ V +     C+    +L     A LK++TISAAFIGLCLMS+INFATA
Sbjct: 558 WGTTSSSLLLITFVTMP---GCSPFSSRLHGTNWAILKSVTISAAFIGLCLMSIINFATA 617

Query: 601 ELGAFFMVSMCLMAHPLKLDLMAGSFKALSRAACNIVLGSIAFPPIAFFLFKGSL-QGFH 660
           E+GA  +V  CLMA P+K  L +   K+L  A C+IVL +I FP + F + KG L +G  
Sbjct: 618 EIGALLLVPTCLMAQPIKPALRSRRLKSLLGAFCSIVLLTIGFPVMFFAISKGLLGEGLV 677

Query: 661 NLHI-GDFWNWMETLWAWNSATFLYLGMVHLPCWALCIQILLHP 693
            L + G+FW W+E+LWAW SAT+LY+GMVHLPCW LC+ IL HP
Sbjct: 678 GLSLGGEFWTWLESLWAWKSATYLYIGMVHLPCWLLCLCILFHP 695

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9WTK32.7e-2824.40Glycosylphosphatidylinositol anchor attachment 1 protein OS=Mus musculus OX=1009... [more]
O432923.0e-2423.75Glycosylphosphatidylinositol anchor attachment 1 protein OS=Homo sapiens OX=9606... [more]
Q9US481.7e-1424.54GPI transamidase component gaa1 OS=Schizosaccharomyces pombe (strain 972 / ATCC ... [more]
P390121.7e-1425.17GPI transamidase component GAA1 OS=Saccharomyces cerevisiae (strain ATCC 204508 ... [more]
Match NameE-valueIdentityDescription
XP_022133688.10.091.82LOW QUALITY PROTEIN: glycosylphosphatidylinositol anchor attachment 1 protein-li... [more]
XP_022144229.10.092.52LOW QUALITY PROTEIN: glycosylphosphatidylinositol anchor attachment 1 protein-li... [more]
XP_023550991.10.084.04glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 [Cucurb... [more]
XP_038891214.10.083.76glycosylphosphatidylinositol anchor attachment 1 protein isoform X1 [Benincasa h... [more]
KAG7016207.10.083.33Glycosylphosphatidylinositol anchor attachment 1 protein [Cucurbita argyrosperma... [more]
Match NameE-valueIdentityDescription
A0A6J1BZU70.091.96LOW QUALITY PROTEIN: glycosylphosphatidylinositol anchor attachment 1 protein-li... [more]
A0A6J1CRQ00.092.52LOW QUALITY PROTEIN: glycosylphosphatidylinositol anchor attachment 1 protein-li... [more]
A0A6J1FHQ40.083.33glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 OS=Cucu... [more]
A0A6J1K1W50.083.76glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 OS=Cucu... [more]
A0A0A0KP050.083.17Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G523060 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G19130.14.9e-21957.53GPI transamidase component family protein / Gaa1-like family protein [more]
AT5G19130.23.2e-21857.67GPI transamidase component family protein / Gaa1-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007246GPI transamidase component Gaa1PIRSFPIRSF036762GAA1coord: 1..691
e-value: 1.1E-182
score: 606.7
IPR007246GPI transamidase component Gaa1PFAMPF04114Gaa1coord: 139..684
e-value: 1.7E-99
score: 334.1
IPR007246GPI transamidase component Gaa1PANTHERPTHR13304GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEINcoord: 3..689
NoneNo IPR availablePANTHERPTHR13304:SF0GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEINcoord: 3..689

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g0642.1MC08g0642.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016255 attachment of GPI anchor to protein
cellular_component GO:0042765 GPI-anchor transamidase complex
cellular_component GO:0016021 integral component of membrane