MC08g0334 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g0334
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionProteasome subunit beta
LocationMC08: 2612895 .. 2616387 (-)
RNA-Seq ExpressionMC08g0334
SyntenyMC08g0334
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCTCAATGCTCCTCACTCCATGGGTACCACCATCATCGGCGTCACTTACGACGGCGGCGTCGTCCTCGGCGCCGATTCTCGTGCCAGCACTGGTAAATTTCTCTCATCTACTCTGTAGCCCTAATTTCTTAGCGTGCTAGTCTTCTTTTATACGAATATTATCTTCAAGAAACGTTTGAAATGATTAAATGCTAGTGGAGGGAAGTTGTAGTTTGCTGATTTTTAGTTTCTGATCTCTCGACCTTGGAAGCAAGTCATTTTGTATCCATCAGTCTTGGAATTAAGCTTGACGTTAGTTGTTTTGAGTTTTTATGAGTAGATGAAAACATAGAAAGAGTTGGTTTCTCGCGTATTTTCCCCCTGATTGACATCTAGTGACAGCAATTTGGTGAATTTTGTTGTTGGTTCGATCCTCTCTATGTAATCTCATGCATTTTTTCAGAACATTACATGGCTGAAATTTAGGCGGGCTTTTCCAAGCTTAGCTGCTTAAAATGACATGCTTTACAAAAAACTATTTGTTTGCTTCCGAGATGGTTGCAGAAAAATTATGAATGGGATGCAATAAACGAACTACATCCTTGACAAAATTATGTAGTGGAGGAAAAATTAACTTGTAAATAACAGCTACCAAATTATGTTTACATTAGCTTGTTGTCCTGAAACATTTTTTTTTCCAACTCCGTAGCAATCTTAAATAACACAATTATACTTGAAATGGTAAAATGAATCTTAGTTGGTTCTTTCCGGCCTTTTTTTGTATGTGCCTGCTTTCTTGCGCTGTGTTGATGTTGACTAATTTTTTATTTTCCTTGTGATAGGCTTCTATGTGCCTAATCCTGCATCAGACAAGTTTACGAAGCTCTCTGACAACGTCTATGTTTGTCGGTCTGGCTCGGTATTTCCATTTCTGTTATCTCACATATTGAACTGTCTGTCGATTTTGATGGTGAAAAGGCTTCAATAGTTTTTGCTCTGGTTGGCTTTAGTGTTCAGTTCTAGCCTTAAATTTTCAAGTTTTTTCTTTTAACTTCTCGTTCTATAATTAAAATGTGTAAGAGTGTCTCAGTTCATAGGTGTTTTCTCTTGTTTCATTAATGGTTTATTTTGTGCCTCTTGCAGGCTGCAAATTCTCATCTTGTCTTTGCTTATGTGCGTTGCTTCCTTGATACGTGAGTGCTTTAATTACTAAAAATATTATGTTGCAACTTTGAAATGTTGGCTAAATGTAACTTGATCACTTCGAGCGATTTTATTTTTTGCTTGAAAATTTGTGATTGACTTTGGAAGGTTCTGAATGCAACTGCATTATTTTTCATCATGATTTTGTTATGATTCTTTTTTATATTTATTATTATTATTATTCTTTTTATTATTCAGTTTCAGAATTTTCTCTTTGCTGACACAAGTCAATGAAGATGATTTGTACTCATTTCTGTGATTTCTTGTTTTTGTTTTCATTAATATTAATTTATGTGATATAAGTAATGAAGCCACTTGAATTTATATTCAACTTTTTATGTGTTGAGCTCTTACTGATTGTATTTTACCTTTGATGCACAGGATGAAGCGTTTACATGTCCGGCAGCGTGCGACTGTTAAAGGGGCTGCACACTTCGTCCATCATGTATTAAATACCCACCACAAGGTAGGAGCTCATATTGGTTTATTCAGAATTATTGCCTGGTAAATATGGATAAGAAACATTAGCTTTTTATTGAAAGATTGCAAATTGTCCCAATCCAGCCAAAGAGTTAGAAAAAAGCTATCCAATTTGCCAAAATCATGGAAATAGAATAATCACATATAGCAAGACAGGAGAAACCTTTTATTTCATTGAAAATCCTATTGTTTCTTTCCAACCAGGGTCCATAAAATAGCCAGAATCATATTGATCCATAGGATCCTTCTCTTTTCTGTGAAAGGGCGACTGCCATACGACCTGTTGAAGCTGCTAGTCCAAGCTCGACATTAAGATTTGCCTGTTTATGGGCAGTAGCAATGGCTTTGATCCCCAAAGTGTTGGCATTTCCTATGTTGAGTGTGGCCATAATCTTGTTCTTGATTCAAATTGAACCCATGCTTTAACATCAATGTCATTTGGTCGAATGGGCAGGATATGTTACAAACTGGCCTTATTGTTGGTGGATGGGACAAGTATGAAGGTGGTCGAATCTATGGTATTCCTCTTGGTGGAACAATAATAGAGCAGCCATTTGCTATTGGAGGTAAAACCTTTTTTGCCAATTGCTTATAGAAAATAACGAGGTTTGGTGACTCTTTTTTTTTTTCCTGGTATCCTTGTGCTTTCTTTTTACTTATGTGTTTATATGCAAGTTTTATGATATGATCTTCTGCTTTAGCATCCTTGAATGAATGAATTGTATTTGGTTTTTCATGATCCTGAATCTGGTTCTTGTTAAACTGAACCCATATAGGGTTCTCAAGCATGGAAGTGCTTTAGTCAAGAAAATATCCTCCTACATTTTTCTTTTGGATATTAGATGTCTGACATTTGCTGTTAAAATCCTATGTTCTCTGGTTGCAAGTATGACCACGACCTGTTTCCACTTGTTATTCTACAGGATCTGGCTCCAGTTACTTGTATGGATTTTTTGATCAAGCTTGGAGAGAAGGAATGACCAGGGAAGAAGCTGAGGTATTTGTATACTACTATTATTTTTTGATTAAAGGTTCTGCATTATTATGCTTCTTGCTTGCTGACAATATTATTTTCTGAGTACTTTCTTTTGGGCTCGGACCATTACTAAATGTATGATTATTTACCTGTTCTTCATTCCTCATAGTTGGAAGTTTACCTGTAAGAGACTTTATTTTTAGTAGTTAGGACTTTTAAGAAACGTTGTAAAATACTTTCTTTTTCTTTTAATAAGTACTTTTGAAGGAAGCACTAATTAAGTAAAATACTTAAAGTTGAGCAGTTACTTCTTTTTCTCTTAGCACTCTCAAGTGCAATACTTCTCTCTAACCACTTTAAAGGAAGCACTAATTAAGAGGCAAAAGATTGAATTCTATGCACACTTCCTATGGCTAAAGCAAATCGGCTGAGCTTACTTTCTGTCTATTTTTTCATTATTCGGTGCAAGAAGATTTAGTGGTTATACTCTGTCGATAGGATTATTTTTCTGATTGTTTACTATCATTTGCAGCAATTGGTGGTCAAGGCGGTTTCTCTTGCCATAGCCAGAGATGGTGCTAGTGGTGGTGTTGTTCGTACTGTTACTGTAAGATAATGTCCTTTTATCACAAAATCTGTTACTTTACTTTAAAACAAGTACACGTACAATCCATTGTTCTTGTTGTGAAGAAGCGTAGTGTAAACTCGGGCATTTTTGCAGATAAATTCCGAGGGAGTGACAAGAAAAGTCTATCCTGGGGACCAGCTTCCGCTGTGGCACGAGGAAATAGAGCCTAAAAATTCATTGCTGGATGTTCTGAATGCTTCTAGTCCCGAACCGATGAACATA

mRNA sequence

ATGGATCTCAATGCTCCTCACTCCATGGGTACCACCATCATCGGCGTCACTTACGACGGCGGCGTCGTCCTCGGCGCCGATTCTCGTGCCAGCACTGGCTTCTATGTGCCTAATCCTGCATCAGACAAGTTTACGAAGCTCTCTGACAACGTCTATGTTTGTCGGTCTGGCTCGGCTGCAAATTCTCATCTTGTCTTTGCTTATGTGCGTTGCTTCCTTGATACGATGAAGCGTTTACATGTCCGGCAGCGTGCGACTGTTAAAGGGGCTGCACACTTCGTCCATCATGTATTAAATACCCACCACAAGGATATGTTACAAACTGGCCTTATTGTTGGTGGATGGGACAAGTATGAAGGTGGTCGAATCTATGGTATTCCTCTTGGTGGAACAATAATAGAGCAGCCATTTGCTATTGGAGGATCTGGCTCCAGTTACTTGTATGGATTTTTTGATCAAGCTTGGAGAGAAGGAATGACCAGGGAAGAAGCTGAGCAATTGGTGGTCAAGGCGGTTTCTCTTGCCATAGCCAGAGATGGTGCTAGTGGTGGTGTTGTTCGTACTGTTACTATAAATTCCGAGGGAGTGACAAGAAAAGTCTATCCTGGGGACCAGCTTCCGCTGTGGCACGAGGAAATAGAGCCTAAAAATTCATTGCTGGATGTTCTGAATGCTTCTAGTCCCGAACCGATGAACATA

Coding sequence (CDS)

ATGGATCTCAATGCTCCTCACTCCATGGGTACCACCATCATCGGCGTCACTTACGACGGCGGCGTCGTCCTCGGCGCCGATTCTCGTGCCAGCACTGGCTTCTATGTGCCTAATCCTGCATCAGACAAGTTTACGAAGCTCTCTGACAACGTCTATGTTTGTCGGTCTGGCTCGGCTGCAAATTCTCATCTTGTCTTTGCTTATGTGCGTTGCTTCCTTGATACGATGAAGCGTTTACATGTCCGGCAGCGTGCGACTGTTAAAGGGGCTGCACACTTCGTCCATCATGTATTAAATACCCACCACAAGGATATGTTACAAACTGGCCTTATTGTTGGTGGATGGGACAAGTATGAAGGTGGTCGAATCTATGGTATTCCTCTTGGTGGAACAATAATAGAGCAGCCATTTGCTATTGGAGGATCTGGCTCCAGTTACTTGTATGGATTTTTTGATCAAGCTTGGAGAGAAGGAATGACCAGGGAAGAAGCTGAGCAATTGGTGGTCAAGGCGGTTTCTCTTGCCATAGCCAGAGATGGTGCTAGTGGTGGTGTTGTTCGTACTGTTACTATAAATTCCGAGGGAGTGACAAGAAAAGTCTATCCTGGGGACCAGCTTCCGCTGTGGCACGAGGAAATAGAGCCTAAAAATTCATTGCTGGATGTTCTGAATGCTTCTAGTCCCGAACCGATGAACATA

Protein sequence

MDLNAPHSMGTTIIGVTYDGGVVLGADSRASTGFYVPNPASDKFTKLSDNVYVCRSGSAANSHLVFAYVRCFLDTMKRLHVRQRATVKGAAHFVHHVLNTHHKDMLQTGLIVGGWDKYEGGRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDGASGGVVRTVTINSEGVTRKVYPGDQLPLWHEEIEPKNSLLDVLNASSPEPMNI
Homology
BLAST of MC08g0334 vs. ExPASy Swiss-Prot
Match: Q8LD27 (Proteasome subunit beta type-6 OS=Arabidopsis thaliana OX=3702 GN=PBA1 PE=1 SV=2)

HSP 1 Score: 349.4 bits (895), Expect = 3.2e-95
Identity = 175/231 (75.76%), Postives = 201/231 (87.01%), Query Frame = 0

Query: 1   MDLNAPHSMGTTIIGVTYDGGVVLGADSRASTGFYVPNPASDKFTKLSDNVYVCRSGSAA 60
           ++L+APHSMGTTIIGVTY+GGVVLGADSR STG YV N ASDK T+L+DNVYVCRSGSAA
Sbjct: 3   LNLDAPHSMGTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAA 62

Query: 61  NSHLVFAYVRCFLDTMKRLHVRQRATVKGAAHFVHHVLNTHHKDMLQTGLIVGGWDKYEG 120
           +S +V  YVR FL      H  Q ATVK +A+ +  +L  ++K+MLQTGLIVGGWDKYEG
Sbjct: 63  DSQVVSDYVRYFLHQHTIQH-GQPATVKVSANLI-RMLAYNNKNMLQTGLIVGGWDKYEG 122

Query: 121 GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDG 180
           G+IYGIPLGGT++EQPFAIGGSGSSYLYGFFDQAW++ MT+EEAEQLVVKAVSLAIARDG
Sbjct: 123 GKIYGIPLGGTVVEQPFAIGGSGSSYLYGFFDQAWKDNMTKEEAEQLVVKAVSLAIARDG 182

Query: 181 ASGGVVRTVTINSEGVTRKVYPGDQLPLWHEEIEPKNSLLDVLNASSPEPM 232
           ASGGVVRTV INSEGVTR  YPGD+L LWHEE+EP+NSLLD+LNA+ PEPM
Sbjct: 183 ASGGVVRTVIINSEGVTRNFYPGDKLQLWHEELEPQNSLLDILNAAGPEPM 231

BLAST of MC08g0334 vs. ExPASy Swiss-Prot
Match: P93395 (Proteasome subunit beta type-6 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 6.3e-91
Identity = 169/230 (73.48%), Postives = 194/230 (84.35%), Query Frame = 0

Query: 2   DLNAPHSMGTTIIGVTYDGGVVLGADSRASTGFYVPNPASDKFTKLSDNVYVCRSGSAAN 61
           D++ PHSMGTTIIGVTY+GGVVLGADSR STG YV N ASDK T+L+DNVYVCRSGSAA+
Sbjct: 5   DVDQPHSMGTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAAD 64

Query: 62  SHLVFAYVRCFLDTMKRLHVRQRATVKGAAHFVHHVLNTHHKDMLQTGLIVGGWDKYEGG 121
           S +V  YVR FL     + + Q ATVK AA+    +L+ ++KD LQTG+I+GGWDKYEGG
Sbjct: 65  SQIVSDYVRYFLH-QHTIQLGQPATVKVAANLT-RLLSYNNKDRLQTGMIIGGWDKYEGG 124

Query: 122 RIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDGA 181
           +IYGIP GGT++EQPFAIGGSGSSYLYGFFDQAW+EGMT+EEAE+LVV AVSLAIARDGA
Sbjct: 125 KIYGIPPGGTVLEQPFAIGGSGSSYLYGFFDQAWKEGMTQEEAEKLVVTAVSLAIARDGA 184

Query: 182 SGGVVRTVTINSEGVTRKVYPGDQLPLWHEEIEPKNSLLDVLNASSPEPM 232
           SGGVVRTVTIN +G TRK Y GD L LWHEE+EP NSLLDV+ ASSP PM
Sbjct: 185 SGGVVRTVTINKDGATRKFYSGDSLQLWHEELEPVNSLLDVVFASSPVPM 232

BLAST of MC08g0334 vs. ExPASy Swiss-Prot
Match: Q3MHN0 (Proteasome subunit beta type-6 OS=Bos taurus OX=9913 GN=PSMB6 PE=1 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 1.9e-47
Identity = 96/204 (47.06%), Postives = 131/204 (64.22%), Query Frame = 0

Query: 4   NAPHSMGTTIIGVTYDGGVVLGADSRASTGFYVPNPASDKFTKLSDNVYVCRSGSAANSH 63
           N   S GTTI+ V +DGGVVLGADSR +TG Y+ N  +DK T + D ++ CRSGSAA++ 
Sbjct: 28  NREVSTGTTIMAVQFDGGVVLGADSRTTTGSYIANRVTDKLTPIHDRIFCCRSGSAADTQ 87

Query: 64  LVFAYVRCFLDTMKRLHVRQRATVKGAAHFVHHVLNTHHKDMLQTGLIVGGWDKYEGGRI 123
            V   V   L     + + +   V  AA     +   + +D L  G+I+ GWD  EGG++
Sbjct: 88  AVADAVTYQLG-FHSIELNEPPLVHTAASLFKEMCYRYRED-LMAGIIIAGWDPQEGGQV 147

Query: 124 YGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDGASG 183
           Y +P+GG ++ QPFAIGGSGSSY+YG+ D  +REGMT+EE  Q    A++LA+ RDG+SG
Sbjct: 148 YSVPMGGMMVRQPFAIGGSGSSYIYGYVDATYREGMTKEECLQFTANALALAMERDGSSG 207

Query: 184 GVVRTVTINSEGVTRKVYPGDQLP 208
           GV+R   I   GV R+V  GDQ+P
Sbjct: 208 GVIRLAAIAEPGVERQVLLGDQIP 229

BLAST of MC08g0334 vs. ExPASy Swiss-Prot
Match: Q55GJ6 (Proteasome subunit beta type-6 OS=Dictyostelium discoideum OX=44689 GN=psmB6 PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 5.6e-47
Identity = 92/203 (45.32%), Postives = 140/203 (68.97%), Query Frame = 0

Query: 9   MGTTIIGVTYDGGVVLGADSRASTGFYVPNPASDKFTKLSDNVYVCRSGSAANSHLVFAY 68
           +GT+I+ V YDGGV++GADSR +TG Y+ N  ++K T + + +Y CRSGSAA++  +  Y
Sbjct: 13  LGTSIMAVEYDGGVIMGADSRTTTGAYIANRVTNKITPIHERIYCCRSGSAADTQAISDY 72

Query: 69  VRCFLDTMKRLHVRQRATVKGAAHFVHHVLNTHHKDMLQTGLIVGGWDKYEGGRIYGIPL 128
           VR +L+ M    +     VK AA  +  +L   +K+ L  G+IV GWDK++GG +Y I L
Sbjct: 73  VRYYLE-MHTSELCDEPDVKTAAS-LFQLLCYSNKNNLMAGIIVAGWDKHQGGSVYNISL 132

Query: 129 GGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDGASGGVVRT 188
           GG++++QPFAIGGSGS+Y+YG+ D  ++  MT++E  + V  +++LA+ RDG+SGGV+R 
Sbjct: 133 GGSMVKQPFAIGGSGSTYIYGYCDSKFKPKMTKDECIEFVQNSLALAMFRDGSSGGVIRL 192

Query: 189 VTINSEGVTRKVYPGDQLPLWHE 212
             I+  GV RK+ PG+ LP + E
Sbjct: 193 CIIDKNGVERKMIPGNNLPRFWE 213

BLAST of MC08g0334 vs. ExPASy Swiss-Prot
Match: P28073 (Proteasome subunit beta type-6 OS=Rattus norvegicus OX=10116 GN=Psmb6 PE=1 SV=3)

HSP 1 Score: 188.0 bits (476), Expect = 1.2e-46
Identity = 94/204 (46.08%), Postives = 131/204 (64.22%), Query Frame = 0

Query: 4   NAPHSMGTTIIGVTYDGGVVLGADSRASTGFYVPNPASDKFTKLSDNVYVCRSGSAANSH 63
           N   S GTTI+ V +DGGVVLGADSR +TG Y+ N  +DK T + D+++ CRSGSAA++ 
Sbjct: 27  NREVSTGTTIMAVQFDGGVVLGADSRTTTGSYIANRVTDKLTPIHDHIFCCRSGSAADTQ 86

Query: 64  LVFAYVRCFLDTMKRLHVRQRATVKGAAHFVHHVLNTHHKDMLQTGLIVGGWDKYEGGRI 123
            V   V   L     + + +   V  AA     +   + +D L  G+I+ GWD  EGG++
Sbjct: 87  AVADAVTYQLG-FHSIELNEPPLVHTAASLFKEMCYRYRED-LMAGIIIAGWDPQEGGQV 146

Query: 124 YGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDGASG 183
           Y +P+GG ++ Q FAIGGSGSSY+YG+ D  +REGMT++E  Q    A++LA+ RDG+SG
Sbjct: 147 YSVPMGGMMVRQSFAIGGSGSSYIYGYVDATYREGMTKDECLQFTANALALAMERDGSSG 206

Query: 184 GVVRTVTINSEGVTRKVYPGDQLP 208
           GV+R   I   GV R+V  GDQ+P
Sbjct: 207 GVIRLAAIQQSGVERQVLLGDQIP 228

BLAST of MC08g0334 vs. NCBI nr
Match: XP_038883996.1 (proteasome subunit beta type-6 [Benincasa hispida])

HSP 1 Score: 381 bits (979), Expect = 5.02e-132
Identity = 197/233 (84.55%), Postives = 209/233 (89.70%), Query Frame = 0

Query: 1   MDLNAPHSMGTTIIGVTYDGGVVLGADSRASTGFYVPNPASDKFTKLSDNVYVCRSGSAA 60
           MDLNAPHSMGTTIIGVTYDGGV+LGADSR STG YV N ASDK TKL+DNVYVCRSGSAA
Sbjct: 5   MDLNAPHSMGTTIIGVTYDGGVILGADSRTSTGVYVANRASDKITKLTDNVYVCRSGSAA 64

Query: 61  NSHLVFAYVRCFLDTMKRLHVRQRATVKGAAHFVHHVLNTHHKDMLQTGLIVGGWDKYEG 120
           +S +V  YVR FL     + + Q ATVK AA+ V  +L  ++KDMLQTGLIVGGWDKYEG
Sbjct: 65  DSQIVSDYVRHFLH-QHTIQLGQPATVKVAANLVR-LLAYNNKDMLQTGLIVGGWDKYEG 124

Query: 121 GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDG 180
           GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAW+EGMTREEAEQLVVKAVSLAIARDG
Sbjct: 125 GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWKEGMTREEAEQLVVKAVSLAIARDG 184

Query: 181 ASGGVVRTVTINSEGVTRKVYPGDQLPLWHEEIEPKNSLLDVLNASSPEPMNI 233
           ASGGVVRTVTINSEGVTRK YPGDQLPLWHEE+EPKNSLLDVLNASSPEPMNI
Sbjct: 185 ASGGVVRTVTINSEGVTRKFYPGDQLPLWHEELEPKNSLLDVLNASSPEPMNI 235

BLAST of MC08g0334 vs. NCBI nr
Match: XP_022132123.1 (proteasome subunit beta type-6 [Momordica charantia])

HSP 1 Score: 381 bits (979), Expect = 5.02e-132
Identity = 198/233 (84.98%), Postives = 209/233 (89.70%), Query Frame = 0

Query: 1   MDLNAPHSMGTTIIGVTYDGGVVLGADSRASTGFYVPNPASDKFTKLSDNVYVCRSGSAA 60
           MDLNAPHSMGTTIIGVTYDGGVVLGADSR STG YV N ASDK TKL+DNVYVCRSGSAA
Sbjct: 5   MDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRSGSAA 64

Query: 61  NSHLVFAYVRCFLDTMKRLHVRQRATVKGAAHFVHHVLNTHHKDMLQTGLIVGGWDKYEG 120
           +S +V  YVR FL     + + Q ATVK AA+ V  +L+ ++KDMLQTGLIVGGWDKY+G
Sbjct: 65  DSQIVSDYVRHFLH-QHTIQLGQPATVKVAANLVR-LLSYNNKDMLQTGLIVGGWDKYDG 124

Query: 121 GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDG 180
           GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGM REEAEQLVVKAVSLAIARDG
Sbjct: 125 GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMIREEAEQLVVKAVSLAIARDG 184

Query: 181 ASGGVVRTVTINSEGVTRKVYPGDQLPLWHEEIEPKNSLLDVLNASSPEPMNI 233
           ASGGVVRTVTINSEGVTRK YPGDQLPLWHEEIEPKNSLLDVLNASSPEPMNI
Sbjct: 185 ASGGVVRTVTINSEGVTRKFYPGDQLPLWHEEIEPKNSLLDVLNASSPEPMNI 235

BLAST of MC08g0334 vs. NCBI nr
Match: XP_022962546.1 (proteasome subunit beta type-6-like [Cucurbita moschata] >XP_023545809.1 proteasome subunit beta type-6-like [Cucurbita pepo subsp. pepo] >KAG7029736.1 Proteasome subunit beta type-6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 380 bits (977), Expect = 1.01e-131
Identity = 197/233 (84.55%), Postives = 208/233 (89.27%), Query Frame = 0

Query: 1   MDLNAPHSMGTTIIGVTYDGGVVLGADSRASTGFYVPNPASDKFTKLSDNVYVCRSGSAA 60
           MDLNAPHSMGTTIIGVTYDGGVVLGADSR STG YV N ASDK TKL+DNVYVCRSGSAA
Sbjct: 5   MDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRSGSAA 64

Query: 61  NSHLVFAYVRCFLDTMKRLHVRQRATVKGAAHFVHHVLNTHHKDMLQTGLIVGGWDKYEG 120
           +S +V  YVR FL     + + Q ATVK AA+ V  +L  ++KDMLQTGLIVGGWDKY G
Sbjct: 65  DSQIVSDYVRHFLH-QHTIQLGQPATVKVAANLVR-LLAYNNKDMLQTGLIVGGWDKYNG 124

Query: 121 GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDG 180
           GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDG
Sbjct: 125 GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDG 184

Query: 181 ASGGVVRTVTINSEGVTRKVYPGDQLPLWHEEIEPKNSLLDVLNASSPEPMNI 233
           ASGGVVRTVTINSEGVTRK YPGDQLPLWHEE+EPKNSLLDVLNASSPEPMN+
Sbjct: 185 ASGGVVRTVTINSEGVTRKFYPGDQLPLWHEELEPKNSLLDVLNASSPEPMNV 235

BLAST of MC08g0334 vs. NCBI nr
Match: XP_022996805.1 (proteasome subunit beta type-6-like [Cucurbita maxima])

HSP 1 Score: 379 bits (972), Expect = 5.85e-131
Identity = 196/233 (84.12%), Postives = 207/233 (88.84%), Query Frame = 0

Query: 1   MDLNAPHSMGTTIIGVTYDGGVVLGADSRASTGFYVPNPASDKFTKLSDNVYVCRSGSAA 60
           MDLNAPHSMGTTIIGVTYDGGVVLGADSR STG YV N ASDK TKL+DNVYVCRSGSAA
Sbjct: 5   MDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRSGSAA 64

Query: 61  NSHLVFAYVRCFLDTMKRLHVRQRATVKGAAHFVHHVLNTHHKDMLQTGLIVGGWDKYEG 120
           +S +V  YVR FL     + + Q ATVK AA+ V  +L  ++KDMLQTGLIVGGWDKY G
Sbjct: 65  DSQIVSDYVRHFLH-QHTIQLGQPATVKVAANLVR-LLAYNNKDMLQTGLIVGGWDKYNG 124

Query: 121 GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDG 180
           GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDG
Sbjct: 125 GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDG 184

Query: 181 ASGGVVRTVTINSEGVTRKVYPGDQLPLWHEEIEPKNSLLDVLNASSPEPMNI 233
           ASGGVVRTVTINSEGV RK YPGDQLPLWHEE+EPKNSLLDVLNASSPEPMN+
Sbjct: 185 ASGGVVRTVTINSEGVARKFYPGDQLPLWHEELEPKNSLLDVLNASSPEPMNV 235

BLAST of MC08g0334 vs. NCBI nr
Match: XP_022951437.1 (proteasome subunit beta type-6 [Cucurbita moschata] >XP_023002488.1 proteasome subunit beta type-6 [Cucurbita maxima] >XP_023537465.1 proteasome subunit beta type-6 [Cucurbita pepo subsp. pepo] >KAG7020325.1 Proteasome subunit beta type-6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 377 bits (968), Expect = 2.38e-130
Identity = 195/233 (83.69%), Postives = 208/233 (89.27%), Query Frame = 0

Query: 1   MDLNAPHSMGTTIIGVTYDGGVVLGADSRASTGFYVPNPASDKFTKLSDNVYVCRSGSAA 60
           MDLNAPHSMGTTIIGVTY+GGVVLGADSR STG YV N ASDK TKL+DNVYVCRSGSAA
Sbjct: 5   MDLNAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRSGSAA 64

Query: 61  NSHLVFAYVRCFLDTMKRLHVRQRATVKGAAHFVHHVLNTHHKDMLQTGLIVGGWDKYEG 120
           +S +V  YVR FL     + + Q ATVK AA+ V  +L  ++KDMLQTGLIVGGWDKYEG
Sbjct: 65  DSQIVSDYVRHFLH-QHTIQLGQPATVKVAANLVR-LLAYNNKDMLQTGLIVGGWDKYEG 124

Query: 121 GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDG 180
           GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAW+EGMTR+EAEQLVVKAVSLAIARDG
Sbjct: 125 GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWKEGMTRDEAEQLVVKAVSLAIARDG 184

Query: 181 ASGGVVRTVTINSEGVTRKVYPGDQLPLWHEEIEPKNSLLDVLNASSPEPMNI 233
           ASGGVVRTVTINSEGVTRK YPGDQLPLWHEE+EPKNSLLD LNASSPEPMNI
Sbjct: 185 ASGGVVRTVTINSEGVTRKFYPGDQLPLWHEELEPKNSLLDALNASSPEPMNI 235

BLAST of MC08g0334 vs. ExPASy TrEMBL
Match: A0A6J1BVE0 (Proteasome subunit beta OS=Momordica charantia OX=3673 GN=LOC111005067 PE=3 SV=1)

HSP 1 Score: 381 bits (979), Expect = 2.43e-132
Identity = 198/233 (84.98%), Postives = 209/233 (89.70%), Query Frame = 0

Query: 1   MDLNAPHSMGTTIIGVTYDGGVVLGADSRASTGFYVPNPASDKFTKLSDNVYVCRSGSAA 60
           MDLNAPHSMGTTIIGVTYDGGVVLGADSR STG YV N ASDK TKL+DNVYVCRSGSAA
Sbjct: 5   MDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRSGSAA 64

Query: 61  NSHLVFAYVRCFLDTMKRLHVRQRATVKGAAHFVHHVLNTHHKDMLQTGLIVGGWDKYEG 120
           +S +V  YVR FL     + + Q ATVK AA+ V  +L+ ++KDMLQTGLIVGGWDKY+G
Sbjct: 65  DSQIVSDYVRHFLH-QHTIQLGQPATVKVAANLVR-LLSYNNKDMLQTGLIVGGWDKYDG 124

Query: 121 GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDG 180
           GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGM REEAEQLVVKAVSLAIARDG
Sbjct: 125 GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMIREEAEQLVVKAVSLAIARDG 184

Query: 181 ASGGVVRTVTINSEGVTRKVYPGDQLPLWHEEIEPKNSLLDVLNASSPEPMNI 233
           ASGGVVRTVTINSEGVTRK YPGDQLPLWHEEIEPKNSLLDVLNASSPEPMNI
Sbjct: 185 ASGGVVRTVTINSEGVTRKFYPGDQLPLWHEEIEPKNSLLDVLNASSPEPMNI 235

BLAST of MC08g0334 vs. ExPASy TrEMBL
Match: A0A6J1HDK0 (Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111462944 PE=3 SV=1)

HSP 1 Score: 380 bits (977), Expect = 4.90e-132
Identity = 197/233 (84.55%), Postives = 208/233 (89.27%), Query Frame = 0

Query: 1   MDLNAPHSMGTTIIGVTYDGGVVLGADSRASTGFYVPNPASDKFTKLSDNVYVCRSGSAA 60
           MDLNAPHSMGTTIIGVTYDGGVVLGADSR STG YV N ASDK TKL+DNVYVCRSGSAA
Sbjct: 5   MDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRSGSAA 64

Query: 61  NSHLVFAYVRCFLDTMKRLHVRQRATVKGAAHFVHHVLNTHHKDMLQTGLIVGGWDKYEG 120
           +S +V  YVR FL     + + Q ATVK AA+ V  +L  ++KDMLQTGLIVGGWDKY G
Sbjct: 65  DSQIVSDYVRHFLH-QHTIQLGQPATVKVAANLVR-LLAYNNKDMLQTGLIVGGWDKYNG 124

Query: 121 GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDG 180
           GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDG
Sbjct: 125 GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDG 184

Query: 181 ASGGVVRTVTINSEGVTRKVYPGDQLPLWHEEIEPKNSLLDVLNASSPEPMNI 233
           ASGGVVRTVTINSEGVTRK YPGDQLPLWHEE+EPKNSLLDVLNASSPEPMN+
Sbjct: 185 ASGGVVRTVTINSEGVTRKFYPGDQLPLWHEELEPKNSLLDVLNASSPEPMNV 235

BLAST of MC08g0334 vs. ExPASy TrEMBL
Match: A0A6J1K7T1 (Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111491932 PE=3 SV=1)

HSP 1 Score: 379 bits (972), Expect = 2.83e-131
Identity = 196/233 (84.12%), Postives = 207/233 (88.84%), Query Frame = 0

Query: 1   MDLNAPHSMGTTIIGVTYDGGVVLGADSRASTGFYVPNPASDKFTKLSDNVYVCRSGSAA 60
           MDLNAPHSMGTTIIGVTYDGGVVLGADSR STG YV N ASDK TKL+DNVYVCRSGSAA
Sbjct: 5   MDLNAPHSMGTTIIGVTYDGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRSGSAA 64

Query: 61  NSHLVFAYVRCFLDTMKRLHVRQRATVKGAAHFVHHVLNTHHKDMLQTGLIVGGWDKYEG 120
           +S +V  YVR FL     + + Q ATVK AA+ V  +L  ++KDMLQTGLIVGGWDKY G
Sbjct: 65  DSQIVSDYVRHFLH-QHTIQLGQPATVKVAANLVR-LLAYNNKDMLQTGLIVGGWDKYNG 124

Query: 121 GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDG 180
           GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDG
Sbjct: 125 GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDG 184

Query: 181 ASGGVVRTVTINSEGVTRKVYPGDQLPLWHEEIEPKNSLLDVLNASSPEPMNI 233
           ASGGVVRTVTINSEGV RK YPGDQLPLWHEE+EPKNSLLDVLNASSPEPMN+
Sbjct: 185 ASGGVVRTVTINSEGVARKFYPGDQLPLWHEELEPKNSLLDVLNASSPEPMNV 235

BLAST of MC08g0334 vs. ExPASy TrEMBL
Match: A0A6J1KTP7 (Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111496312 PE=3 SV=1)

HSP 1 Score: 377 bits (968), Expect = 1.15e-130
Identity = 195/233 (83.69%), Postives = 208/233 (89.27%), Query Frame = 0

Query: 1   MDLNAPHSMGTTIIGVTYDGGVVLGADSRASTGFYVPNPASDKFTKLSDNVYVCRSGSAA 60
           MDLNAPHSMGTTIIGVTY+GGVVLGADSR STG YV N ASDK TKL+DNVYVCRSGSAA
Sbjct: 5   MDLNAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRSGSAA 64

Query: 61  NSHLVFAYVRCFLDTMKRLHVRQRATVKGAAHFVHHVLNTHHKDMLQTGLIVGGWDKYEG 120
           +S +V  YVR FL     + + Q ATVK AA+ V  +L  ++KDMLQTGLIVGGWDKYEG
Sbjct: 65  DSQIVSDYVRHFLH-QHTIQLGQPATVKVAANLVR-LLAYNNKDMLQTGLIVGGWDKYEG 124

Query: 121 GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDG 180
           GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAW+EGMTR+EAEQLVVKAVSLAIARDG
Sbjct: 125 GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWKEGMTRDEAEQLVVKAVSLAIARDG 184

Query: 181 ASGGVVRTVTINSEGVTRKVYPGDQLPLWHEEIEPKNSLLDVLNASSPEPMNI 233
           ASGGVVRTVTINSEGVTRK YPGDQLPLWHEE+EPKNSLLD LNASSPEPMNI
Sbjct: 185 ASGGVVRTVTINSEGVTRKFYPGDQLPLWHEELEPKNSLLDALNASSPEPMNI 235

BLAST of MC08g0334 vs. ExPASy TrEMBL
Match: A0A6J1GHL6 (Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111454253 PE=3 SV=1)

HSP 1 Score: 377 bits (968), Expect = 1.15e-130
Identity = 195/233 (83.69%), Postives = 208/233 (89.27%), Query Frame = 0

Query: 1   MDLNAPHSMGTTIIGVTYDGGVVLGADSRASTGFYVPNPASDKFTKLSDNVYVCRSGSAA 60
           MDLNAPHSMGTTIIGVTY+GGVVLGADSR STG YV N ASDK TKL+DNVYVCRSGSAA
Sbjct: 5   MDLNAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITKLTDNVYVCRSGSAA 64

Query: 61  NSHLVFAYVRCFLDTMKRLHVRQRATVKGAAHFVHHVLNTHHKDMLQTGLIVGGWDKYEG 120
           +S +V  YVR FL     + + Q ATVK AA+ V  +L  ++KDMLQTGLIVGGWDKYEG
Sbjct: 65  DSQIVSDYVRHFLH-QHTIQLGQPATVKVAANLVR-LLAYNNKDMLQTGLIVGGWDKYEG 124

Query: 121 GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDG 180
           GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAW+EGMTR+EAEQLVVKAVSLAIARDG
Sbjct: 125 GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWKEGMTRDEAEQLVVKAVSLAIARDG 184

Query: 181 ASGGVVRTVTINSEGVTRKVYPGDQLPLWHEEIEPKNSLLDVLNASSPEPMNI 233
           ASGGVVRTVTINSEGVTRK YPGDQLPLWHEE+EPKNSLLD LNASSPEPMNI
Sbjct: 185 ASGGVVRTVTINSEGVTRKFYPGDQLPLWHEELEPKNSLLDALNASSPEPMNI 235

BLAST of MC08g0334 vs. TAIR 10
Match: AT4G31300.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 349.4 bits (895), Expect = 2.3e-96
Identity = 175/231 (75.76%), Postives = 201/231 (87.01%), Query Frame = 0

Query: 1   MDLNAPHSMGTTIIGVTYDGGVVLGADSRASTGFYVPNPASDKFTKLSDNVYVCRSGSAA 60
           ++L+APHSMGTTIIGVTY+GGVVLGADSR STG YV N ASDK T+L+DNVYVCRSGSAA
Sbjct: 3   LNLDAPHSMGTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAA 62

Query: 61  NSHLVFAYVRCFLDTMKRLHVRQRATVKGAAHFVHHVLNTHHKDMLQTGLIVGGWDKYEG 120
           +S +V  YVR FL      H  Q ATVK +A+ +  +L  ++K+MLQTGLIVGGWDKYEG
Sbjct: 63  DSQVVSDYVRYFLHQHTIQH-GQPATVKVSANLI-RMLAYNNKNMLQTGLIVGGWDKYEG 122

Query: 121 GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDG 180
           G+IYGIPLGGT++EQPFAIGGSGSSYLYGFFDQAW++ MT+EEAEQLVVKAVSLAIARDG
Sbjct: 123 GKIYGIPLGGTVVEQPFAIGGSGSSYLYGFFDQAWKDNMTKEEAEQLVVKAVSLAIARDG 182

Query: 181 ASGGVVRTVTINSEGVTRKVYPGDQLPLWHEEIEPKNSLLDVLNASSPEPM 232
           ASGGVVRTV INSEGVTR  YPGD+L LWHEE+EP+NSLLD+LNA+ PEPM
Sbjct: 183 ASGGVVRTVIINSEGVTRNFYPGDKLQLWHEELEPQNSLLDILNAAGPEPM 231

BLAST of MC08g0334 vs. TAIR 10
Match: AT4G31300.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 349.4 bits (895), Expect = 2.3e-96
Identity = 173/231 (74.89%), Postives = 199/231 (86.15%), Query Frame = 0

Query: 1   MDLNAPHSMGTTIIGVTYDGGVVLGADSRASTGFYVPNPASDKFTKLSDNVYVCRSGSAA 60
           ++L+APHSMGTTIIGVTY+GGVVLGADSR STG YV N ASDK T+L+DNVYVCRSGSAA
Sbjct: 3   LNLDAPHSMGTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAA 62

Query: 61  NSHLVFAYVRCFLDTMKRLHVRQRATVKGAAHFVHHVLNTHHKDMLQTGLIVGGWDKYEG 120
           +S +V  YVR FL      H  Q ATVK +A+ +  +   + ++MLQTGLIVGGWDKYEG
Sbjct: 63  DSQVVSDYVRYFLHQHTIQH-GQPATVKVSANLIRMLAYNNKQNMLQTGLIVGGWDKYEG 122

Query: 121 GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDG 180
           G+IYGIPLGGT++EQPFAIGGSGSSYLYGFFDQAW++ MT+EEAEQLVVKAVSLAIARDG
Sbjct: 123 GKIYGIPLGGTVVEQPFAIGGSGSSYLYGFFDQAWKDNMTKEEAEQLVVKAVSLAIARDG 182

Query: 181 ASGGVVRTVTINSEGVTRKVYPGDQLPLWHEEIEPKNSLLDVLNASSPEPM 232
           ASGGVVRTV INSEGVTR  YPGD+L LWHEE+EP+NSLLD+LNA+ PEPM
Sbjct: 183 ASGGVVRTVIINSEGVTRNFYPGDKLQLWHEELEPQNSLLDILNAAGPEPM 232

BLAST of MC08g0334 vs. TAIR 10
Match: AT4G31300.3 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 349.4 bits (895), Expect = 2.3e-96
Identity = 175/231 (75.76%), Postives = 201/231 (87.01%), Query Frame = 0

Query: 1   MDLNAPHSMGTTIIGVTYDGGVVLGADSRASTGFYVPNPASDKFTKLSDNVYVCRSGSAA 60
           ++L+APHSMGTTIIGVTY+GGVVLGADSR STG YV N ASDK T+L+DNVYVCRSGSAA
Sbjct: 3   LNLDAPHSMGTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAA 62

Query: 61  NSHLVFAYVRCFLDTMKRLHVRQRATVKGAAHFVHHVLNTHHKDMLQTGLIVGGWDKYEG 120
           +S +V  YVR FL      H  Q ATVK +A+ +  +L  ++K+MLQTGLIVGGWDKYEG
Sbjct: 63  DSQVVSDYVRYFLHQHTIQH-GQPATVKVSANLI-RMLAYNNKNMLQTGLIVGGWDKYEG 122

Query: 121 GRIYGIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDG 180
           G+IYGIPLGGT++EQPFAIGGSGSSYLYGFFDQAW++ MT+EEAEQLVVKAVSLAIARDG
Sbjct: 123 GKIYGIPLGGTVVEQPFAIGGSGSSYLYGFFDQAWKDNMTKEEAEQLVVKAVSLAIARDG 182

Query: 181 ASGGVVRTVTINSEGVTRKVYPGDQLPLWHEEIEPKNSLLDVLNASSPEPM 232
           ASGGVVRTV INSEGVTR  YPGD+L LWHEE+EP+NSLLD+LNA+ PEPM
Sbjct: 183 ASGGVVRTVIINSEGVTRNFYPGDKLQLWHEELEPQNSLLDILNAAGPEPM 231

BLAST of MC08g0334 vs. TAIR 10
Match: AT1G13060.1 (20S proteasome beta subunit E1 )

HSP 1 Score: 100.9 bits (250), Expect = 1.4e-21
Identity = 65/211 (30.81%), Postives = 107/211 (50.71%), Query Frame = 0

Query: 7   HSMGTTIIGVTYDGGVVLGADSRASTGFYVPNPASDKFTKLSDNVYVCRSGSAANSHLVF 66
           H+ GTT +   + GGV++ ADSRAS G Y+ + +  K  +++  +    +G AA+     
Sbjct: 54  HAKGTTTLAFIFKGGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWH 113

Query: 67  AY--VRCFLDTMKRLHVRQRATVKGAAHFVHHVLNTHHKDMLQTGLIVGGWDKYEGGRIY 126
               ++C L  +     ++R +V GA+  + ++L ++    L  G ++ GWD+   G  Y
Sbjct: 114 RNLGIKCRLHELAN---KRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPGLYY 173

Query: 127 GIPLGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDGASGG 186
               GG +    F++ GSGS Y YG  D  ++  M+ EEA +L  +++  A  RDGASGG
Sbjct: 174 VDNEGGRLKGDRFSV-GSGSPYAYGVLDSGYKYDMSVEEASELARRSIYHATFRDGASGG 233

Query: 187 VVRTVTINSEGVTRKVYPGDQLPLWHEEIEP 216
           V     +  EG T+    GD +   H    P
Sbjct: 234 VASVYHVGPEGWTK--LSGDDVGELHYHYYP 258

BLAST of MC08g0334 vs. TAIR 10
Match: AT3G26340.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 92.8 bits (229), Expect = 3.9e-19
Identity = 62/208 (29.81%), Postives = 104/208 (50.00%), Query Frame = 0

Query: 10  GTTIIGVTYDGGVVLGADSRASTGFYVPNPASDKFTKLSDNVYVCRSGSAANSHLVFAY- 69
           GTT +   +  GV++ ADSRAS G Y+ + +  K  +++  +    +G AA+        
Sbjct: 57  GTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNL 116

Query: 70  -VRCFLDTMKRLHVRQRATVKGAAHFVHHVLNTHHKDMLQTGLIVGGWDKYEGGRIYGIP 129
            ++C L  +     ++R +V GA+  + ++L ++    L  G ++ GWD+   G  Y   
Sbjct: 117 GIKCRLHELAN---KRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPGLYYVDN 176

Query: 130 LGGTIIEQPFAIGGSGSSYLYGFFDQAWREGMTREEAEQLVVKAVSLAIARDGASGGVVR 189
            GG +    F++ GSGS Y YG  D  ++  M+ EEA +L  +++  A  RDGASGGV  
Sbjct: 177 EGGRLKGDRFSV-GSGSPYAYGVLDSGYKFDMSVEEASELARRSIYHATFRDGASGGVAS 236

Query: 190 TVTINSEGVTRKVYPGDQLPLWHEEIEP 216
              +  +G T+    GD +   H    P
Sbjct: 237 VYHVGPQGWTK--LSGDDVGELHYHYYP 258

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LD273.2e-9575.76Proteasome subunit beta type-6 OS=Arabidopsis thaliana OX=3702 GN=PBA1 PE=1 SV=2[more]
P933956.3e-9173.48Proteasome subunit beta type-6 OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q3MHN01.9e-4747.06Proteasome subunit beta type-6 OS=Bos taurus OX=9913 GN=PSMB6 PE=1 SV=1[more]
Q55GJ65.6e-4745.32Proteasome subunit beta type-6 OS=Dictyostelium discoideum OX=44689 GN=psmB6 PE=... [more]
P280731.2e-4646.08Proteasome subunit beta type-6 OS=Rattus norvegicus OX=10116 GN=Psmb6 PE=1 SV=3[more]
Match NameE-valueIdentityDescription
XP_038883996.15.02e-13284.55proteasome subunit beta type-6 [Benincasa hispida][more]
XP_022132123.15.02e-13284.98proteasome subunit beta type-6 [Momordica charantia][more]
XP_022962546.11.01e-13184.55proteasome subunit beta type-6-like [Cucurbita moschata] >XP_023545809.1 proteas... [more]
XP_022996805.15.85e-13184.12proteasome subunit beta type-6-like [Cucurbita maxima][more]
XP_022951437.12.38e-13083.69proteasome subunit beta type-6 [Cucurbita moschata] >XP_023002488.1 proteasome s... [more]
Match NameE-valueIdentityDescription
A0A6J1BVE02.43e-13284.98Proteasome subunit beta OS=Momordica charantia OX=3673 GN=LOC111005067 PE=3 SV=1[more]
A0A6J1HDK04.90e-13284.55Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111462944 PE=3 SV=1[more]
A0A6J1K7T12.83e-13184.12Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111491932 PE=3 SV=1[more]
A0A6J1KTP71.15e-13083.69Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111496312 PE=3 SV=1[more]
A0A6J1GHL61.15e-13083.69Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111454253 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G31300.12.3e-9675.76N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT4G31300.22.3e-9674.89N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT4G31300.32.3e-9675.76N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT1G13060.11.4e-2130.8120S proteasome beta subunit E1 [more]
AT3G26340.13.9e-1929.81N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000243Peptidase T1A, proteasome beta-subunitPRINTSPR00141PROTEASOMEcoord: 177..188
score: 58.33
coord: 141..152
score: 66.29
coord: 152..163
score: 44.7
coord: 18..33
score: 60.23
IPR001353Proteasome, subunit alpha/betaPFAMPF00227Proteasomecoord: 9..191
e-value: 3.8E-37
score: 127.6
IPR029055Nucleophile aminohydrolases, N-terminalGENE3D3.60.20.10Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1coord: 11..212
e-value: 1.5E-55
score: 189.8
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 6..214
NoneNo IPR availablePANTHERPTHR11599PROTEASOME SUBUNIT ALPHA/BETAcoord: 3..231
NoneNo IPR availablePANTHERPTHR11599:SF151PROTEASOME SUBUNIT BETAcoord: 3..231
NoneNo IPR availableCDDcd03762proteasome_beta_type_6coord: 11..200
e-value: 2.62181E-95
score: 274.872
IPR023333Proteasome B-type subunitPROSITEPS51476PROTEASOME_BETA_2coord: 10..191
score: 36.079338

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g0334.1MC08g0334.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010498 proteasomal protein catabolic process
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0019774 proteasome core complex, beta-subunit complex
cellular_component GO:0005839 proteasome core complex
molecular_function GO:0004298 threonine-type endopeptidase activity