MC08g0325 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g0325
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionProtein kinase domain-containing protein
LocationMC08: 2554194 .. 2558766 (+)
RNA-Seq ExpressionMC08g0325
SyntenyMC08g0325
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CGCGCGTGAAGTAACAGCCTCGTCTTTCCTGTTCAAATCCTCTCACCTCTTACGATTATCTGTGGTGGTCGGCGAGCTGCTGCATCTCTGTTTCACGAAACTGAGTCGCCGAAACCCTAATTTTTTCCTCATTATCAGAGAGTCATTGCTTGTATTTCAGTTGCACATTCATACACATAGCAACGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGTGAGTGAGCGGTAATTCATGAATGGTAGCCATGGAAGCTGATAGAATGGAGAAAGAACAGCAGCGAACCACCAGGAAACGGCCTCGTTCCGCATGGGACGTCGTGCCCTCCGAACAAGAGGTTGGTTGGATTTGGGTCTTGTGTTTTGGGATTAGATTTTCCAGAGACGACGACGTGTTGAATTATGGAGCCCATGGCAATTAGGGCTTTTATTTCGAGTCGCGAAGATTATGCTTATACGGTTCTGGTAGTCTTACATACGTTTCAACGGTTGCTGGGTGCTTATTTTTCCTTTGTAGGCTCATAAGGCTTTGGTGGCGGGTAAGGATGAGGTGAAAAGACACGCATCGCCCCCGAGAAGAGACGATGATCGCGAAGGACATTATGTATTCAACCTCGGCGAGAATCTCACTCCGAGATGTGAGTGTTTTTTTGTTTGTTCTAACCGGTTTGCATTTTGAATTGGCTTCTGGTTATACCATGATTGTTTGGGTATGGAGTGTGTTAAATCGCGCCCGCATTGGTGGTATTTTCTTCTTATCAGGCTAGGTTTTCACAGTAAATCATAAATAAAATAAAGCTAGTATTTTGTGACTTAACATCACTGGAGCCTTCCTTTTATCTCGTTTGCTTGCGAAAAAGCAAGCTATAGGGAAATTATAAAATTCTTGAAACTTTCTGAGATAACCTCTTTTGGTTGTTGCCACCAGGTGTAAGCTGTTACGACATTCTTTTATTATATCGTTAGTTCTTTATGACGTGCATATTGATCTTGTAGCATAGATCTCTCTTCAACTTCCAGCATGGGTTGAGACTCTTCTTGTGGATTTCATGTTATCATTGAAATCTATTTTTAGCAGGGAAAAGTTCTATATAAGTTTTAAGTTTAAAAAATTCGGGAGTTGAATTCACCTAGGTTTCTTTGCCATGTTGCTGGCGCATGATAAACTCCATATGAGACCCTTTTTTTGGGTGTTTCTGAACAACGATTCCCTTATTTTTTTCTAAATACAAAAATGCGATAAATCAACGAGAAGTTCGTATCTTATTAAAAAAAAAAAAACCAAAAAACCATATGGGATAACTATACAACATTCCTATGCATTTTATGTCCTCTCTGATTTTTGTAATCTACTTTCAACACGCCATTTTTCAGATTTTCCATTCCTTTAATTGTTATTAGGTTTTCTGGTCATTCTTTTATTCTTTTATTTTTTGGTTTTTTGATTTGTTATTACTTGTATCTTAATGGGTGATCGGTGACATGCAGATAAAATCCTCAGCAAGATGGGTGAAGGTTAGCTGTCAAGATCCCTGCTGTCTGCAAGATTTTATGTTTTTGTGATTCTGCTCGAGTCCACTTAGCAAATTGCTAGTGCTGTAGGCACATTTGGTCGAGTTTTGGAATGCTGGGACCGTCAAACTCGAGAATATGTGGCCATTAAGGTAGTTCGAAGCATACGAAAATACCGCGATGCAGCCATGGTTGAGGTGGATATACTTAAACATCTTGCTCAGAATGACAAAGGCAGCTTACGGTATGTATCTATTTCATTATGTTTCGTTCTGGTCTTCAATTCATCATATAACATAATCTAACTCTGCTTTGCAACATGTTCCTCATGCAGTTGTGTGCAGATTAGAAATTGGTTTGACTACCGCAATCACATATGTATAGTAAGTATTTCAGTTTATTTTCATTGATTTTTATCTCTCCTCTCCCTCTCTTGATATTTAAAATGTTGTTTTTTGTAATTCTTTTGATGTCATATGTTCTGGTAACTGAAGTCTGAAGTTAATCTCTTTCGATTATCAAAATGGTGGTGGTATTTCTTTTGCTATTGGACAAACGTTATTGTCAATTATTGTGTACATGCATTCTTCATGCATTTACAAGTTCTTTCGTACATGTGGGCTTATTCATAGAATTGGGAAATTAACACAAACCTTCCTTTGAATGCATTTGAATGGTTTGATCAGGTGTTTGAGAAGCTTGGACCAAGCTTATTTGATTTTCTAAAGAGAAATAAATACTGCCCATTCCCTGTGGACCTTGTTCGAGAGTTTGGACGACAGCTTTTGGAATCTGTAGCATGTCTGTGGATTTATTTTTGACCTCTATATCTTCTCTCTGGTTTTCATTTATCTCGTTTTTTCCTAAAAAGCTCCAAAACCATTTTCTTTGCTCTTTCTTCTTTTCAGGGGTGGAAAAATGTCTTATCATTCCAATCTTCTTTGTTTAACTTGTTTATTTTGCTTCATCATATCTGGCAGATATGCATGATTTACACTTAATCCACACAGACCTGAAGCCAGAAAATATACTTCTTGTGTCTTCTGAATATATAAAGCTTCCTGGCTGTAAGGTATTTAGACATTTCAAATCTGAGAATTTGGGTGAAAACCTAAATATATCTTAAGAACAATGCTTGAAATGATACAGAAGGTTTCGTCGGATGAAACGCAATTCAGGTGCTTGCCCAAGTCTAGTGCAATTAAGCTGATTGATTTTGGTAGTACTGCCTTTGATAATGAGAATCATAGCTCCATTGTTTCTACAAGGCATTACAGAGCCCCTGAGATTATACTAGGTAACTAGTTATGTGGTTGGCTAGTGAAATATGTGTTTGTGTATGGTTTCTTCCTCTCTATCCTTCTCTGATTCCAAACTGAGAAACCTTCGCTGTGGAAACTTTATCCAAAATTTAAATTTATTTATGTTGCTTAGAATAAAGCAGTGTCTGGAGGAGAGGGAGATGAAGCCCAGTAGTAGCAATAGGGCAGATGACAAAATCTCTGTCACAACAATTTATTTATAAATTTACCTTTTAAAATGTGATTTATGGAGCATCCTTGATCCTCCTTATACGGAAGCTAGTTTTTCTCCTATATGTTTGAATAAATTTGACTTCTTTTCTTCGTTACTAAAATTTTTACATCCATTTGGCACAACTCCTATAATCAAAAGCATATCATGTCTCTGCTCTTCCAACAGTTCATACATATGTACGCAGGGCAGAAATGTTTTAAGCTTTTCATATGTCTTCATCACTTTAAAAGAATGAGTTGAATGTGTTTAATATATGATGGAAGCTCCACTCTTGTAACATTTGGGTAGGCAAAATTAATTTTGAGGTTAGAGTGACATTTATGGCCGTAGTCTCCAGAGCAACCATGTTTGCTGCTTGACCCTGTCTTAAACACTTCCAACTCATGGGGGGCCTTATCTTGCAACTTTATCATCAGATAGGCATGAAAGTAATATGACTTTATTGCATATCCCACTTTACTAGATTATATGATCTTTGATTACTGCACTTAGAATCTCTGCAAGGAAATGTCAACAATATTAGATATTGCATCGCAAATGCCTCCTACATATTATACATGTCGTCTTCTCACCTACATTGATAAATGTTTTGCAGGTCTTGGGTGGAGTTATCCATGCGATTTGTGGAGTATTGGTTGCATACTTGTTGAACTGTGCTCGGTTAGTATTTGTTGAACATAACTCCGGTTTGATTATGGAATTTAATATTGGTAAATTGTTAAATTTTGAAATCCTGTAGGGCAAAGCACTGTTCCAGACCCATGAGAACTTGGAGCATTTGGCAATGATGGAGAGGGTGTTAGGACCTCTGCCAGGGCACATGATTCAGAGTGCCGAGTACTGAAACATCCAGATTTCTCTCCATTTATTTCCCCCGTAGGGTTTATAATTTGACTGGTTTTCTATCTGCTTCATGTAGCCAAAACGCTGAAAAATATTTCAAACGAGGGTCACGTCTAAATTGGCCCGAAGGAGCAGTTTCAAGAGAAAGCATTAGAGCTGTGAAGAAGCTTGATCGTTTGAAGGTCTTTATATATTATTATGCTTCTTTTAGTTTTATATGCTAATAGTTCTATAGAAATTATTTACTAACTTGCATAAAATGGTTATCTTTTTGTCTTTAGGATATGGTTTCACAACATCTGGGTTTCTCCAGGTCTCTCTTGACTGATCTGTTGTACGATTTACTGAAATATGACCCTTCAGAACGTCCGACCGCCCGTCAAGCCCTTAACCATCCCTTCTTTAAGAGTATAGCTTGAGAAGGAACACTTCCATGCTAAGTTACATTTGCAATACAAGTTCGTCTGCAGAATACTTCCTTCTTTACTAGGGGAAAAAATTAGATTGATATCGAGATTGCTAGTGCAGACAATGATTTTACTTGTATTTGTTGAAGTATGGCTATTAATGAAAAGCATGTCAATATTGTCAGCAAATTTTTTGCTAGAACTTTTTTTTACGCAGTTGTTTATTAATTTTTAAATAGTGCTAGTACCAAAAATATTCTATTCCAGTTTAGGAGTGC

mRNA sequence

CGCGCGTGAAGTAACAGCCTCGTCTTTCCTGTTCAAATCCTCTCACCTCTTACGATTATCTGTGGTGGTCGGCGAGCTGCTGCATCTCTGTTTCACGAAACTGAGTCGCCGAAACCCTAATTTTTTCCTCATTATCAGAGAGTCATTGCTTGTATTTCAGTTGCACATTCATACACATAGCAACGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGTGAGTGAGCGGTAATTCATGAATGGTAGCCATGGAAGCTGATAGAATGGAGAAAGAACAGCAGCGAACCACCAGGAAACGGCCTCGTTCCGCATGGGACGTCGTGCCCTCCGAACAAGAGGCTCATAAGGCTTTGGTGGCGGGTAAGGATGAGGTGAAAAGACACGCATCGCCCCCGAGAAGAGACGATGATCGCGAAGGACATTATGTATTCAACCTCGGCGAGAATCTCACTCCGAGATATAAAATCCTCAGCAAGATGGGTGAAGGCACATTTGGTCGAGTTTTGGAATGCTGGGACCGTCAAACTCGAGAATATGTGGCCATTAAGGTAGTTCGAAGCATACGAAAATACCGCGATGCAGCCATGGTTGAGGTGGATATACTTAAACATCTTGCTCAGAATGACAAAGGCAGCTTACGTTGTGTGCAGATTAGAAATTGGTTTGACTACCGCAATCACATATGTATAGTGTTTGAGAAGCTTGGACCAAGCTTATTTGATTTTCTAAAGAGAAATAAATACTGCCCATTCCCTGTGGACCTTGTTCGAGAGTTTGGACGACAGCTTTTGGAATCTGTAGCATATATGCATGATTTACACTTAATCCACACAGACCTGAAGCCAGAAAATATACTTCTTGTGTCTTCTGAATATATAAAGCTTCCTGGCTGTAAGAAGGTTTCGTCGGATGAAACGCAATTCAGGTGCTTGCCCAAGTCTAGTGCAATTAAGCTGATTGATTTTGGTAGTACTGCCTTTGATAATGAGAATCATAGCTCCATTGTTTCTACAAGGCATTACAGAGCCCCTGAGATTATACTAGGTCTTGGGTGGAGTTATCCATGCGATTTGTGGAGTATTGGTTGCATACTTGTTGAACTGTGCTCGGGCAAAGCACTGTTCCAGACCCATGAGAACTTGGAGCATTTGGCAATGATGGAGAGGGTGTTAGGACCTCTGCCAGGGCACATGATTCAGAGTGCCGACCAAAACGCTGAAAAATATTTCAAACGAGGGTCACGTCTAAATTGGCCCGAAGGAGCAGTTTCAAGAGAAAGCATTAGAGCTGTGAAGAAGCTTGATCGTTTGAAGGATATGGTTTCACAACATCTGGGTTTCTCCAGGTCTCTCTTGACTGATCTGTTGTACGATTTACTGAAATATGACCCTTCAGAACGTCCGACCGCCCGTCAAGCCCTTAACCATCCCTTCTTTAAGAGTATAGCTTGAGAAGGAACACTTCCATGCTAAGTTACATTTGCAATACAAGTTCGTCTGCAGAATACTTCCTTCTTTACTAGGGGAAAAAATTAGATTGATATCGAGATTGCTAGTGCAGACAATGATTTTACTTGTATTTGTTGAAGTATGGCTATTAATGAAAAGCATGTCAATATTGTCAGCAAATTTTTTGCTAGAACTTTTTTTTACGCAGTTGTTTATTAATTTTTAAATAGTGCTAGTACCAAAAATATTCTATTCCAGTTTAGGAGTGC

Coding sequence (CDS)

ATGGTAGCCATGGAAGCTGATAGAATGGAGAAAGAACAGCAGCGAACCACCAGGAAACGGCCTCGTTCCGCATGGGACGTCGTGCCCTCCGAACAAGAGGCTCATAAGGCTTTGGTGGCGGGTAAGGATGAGGTGAAAAGACACGCATCGCCCCCGAGAAGAGACGATGATCGCGAAGGACATTATGTATTCAACCTCGGCGAGAATCTCACTCCGAGATATAAAATCCTCAGCAAGATGGGTGAAGGCACATTTGGTCGAGTTTTGGAATGCTGGGACCGTCAAACTCGAGAATATGTGGCCATTAAGGTAGTTCGAAGCATACGAAAATACCGCGATGCAGCCATGGTTGAGGTGGATATACTTAAACATCTTGCTCAGAATGACAAAGGCAGCTTACGTTGTGTGCAGATTAGAAATTGGTTTGACTACCGCAATCACATATGTATAGTGTTTGAGAAGCTTGGACCAAGCTTATTTGATTTTCTAAAGAGAAATAAATACTGCCCATTCCCTGTGGACCTTGTTCGAGAGTTTGGACGACAGCTTTTGGAATCTGTAGCATATATGCATGATTTACACTTAATCCACACAGACCTGAAGCCAGAAAATATACTTCTTGTGTCTTCTGAATATATAAAGCTTCCTGGCTGTAAGAAGGTTTCGTCGGATGAAACGCAATTCAGGTGCTTGCCCAAGTCTAGTGCAATTAAGCTGATTGATTTTGGTAGTACTGCCTTTGATAATGAGAATCATAGCTCCATTGTTTCTACAAGGCATTACAGAGCCCCTGAGATTATACTAGGTCTTGGGTGGAGTTATCCATGCGATTTGTGGAGTATTGGTTGCATACTTGTTGAACTGTGCTCGGGCAAAGCACTGTTCCAGACCCATGAGAACTTGGAGCATTTGGCAATGATGGAGAGGGTGTTAGGACCTCTGCCAGGGCACATGATTCAGAGTGCCGACCAAAACGCTGAAAAATATTTCAAACGAGGGTCACGTCTAAATTGGCCCGAAGGAGCAGTTTCAAGAGAAAGCATTAGAGCTGTGAAGAAGCTTGATCGTTTGAAGGATATGGTTTCACAACATCTGGGTTTCTCCAGGTCTCTCTTGACTGATCTGTTGTACGATTTACTGAAATATGACCCTTCAGAACGTCCGACCGCCCGTCAAGCCCTTAACCATCCCTTCTTTAAGAGTATAGCTTGA

Protein sequence

MVAMEADRMEKEQQRTTRKRPRSAWDVVPSEQEAHKALVAGKDEVKRHASPPRRDDDREGHYVFNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILKHLAQNDKGSLRCVQIRNWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFGRQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKKVSSDETQFRCLPKSSAIKLIDFGSTAFDNENHSSIVSTRHYRAPEIILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEHLAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLDRLKDMVSQHLGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA
Homology
[Close]
BLAST of MC08g0325 vs. ExPASy Swiss-Prot
Match: P51568 (Serine/threonine-protein kinase AFC3 OS=Arabidopsis thaliana OX=3702 GN=AFC3 PE=1 SV=2)

HSP 1 Score: 611.3 bits (1575), Expect = 7.9e-174
Identity = 291/401 (72.57%), Postives = 337/401 (84.04%), Query Frame = 0 

Query: 1   MVAMEADRMEKEQQRTTRKRPRSAWDVVPSEQEAHKALVAGKDEVKRHASPPRRDDDREG 60
           M+A   + M+KE+    RKRPR  WD  P+E EA +A++ G     R  SPP RDDDR+G
Sbjct: 1   MIANGFESMDKER---VRKRPRMTWDEAPAEPEAKRAVIKGHGSDGRILSPPLRDDDRDG 60

Query: 61  HYVFNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVD 120
           HYVF+L +NLTPRYKILSKMGEGTFGRVLECWDR T+EYVAIK++RSI+KYRDAAM+E+D
Sbjct: 61  HYVFSLRDNLTPRYKILSKMGEGTFGRVLECWDRDTKEYVAIKIIRSIKKYRDAAMIEID 120

Query: 121 ILKHLAQNDKGSLRCVQIRNWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFG 180
           +L+ L ++DKG  RCVQ++NWFDYRNHICIVFEKLGPSLFDFLKRNKY  FP+ LVR+FG
Sbjct: 121 VLQKLVKSDKGRTRCVQMKNWFDYRNHICIVFEKLGPSLFDFLKRNKYSAFPLALVRDFG 180

Query: 181 RQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKKVSSDETQFRCLPKSSAIKLI 240
            QLLESVAYMH+L L+HTDLKPENILLVSSE +KLP  K+ +++ET FRCLPKSSAIKLI
Sbjct: 181 CQLLESVAYMHELQLVHTDLKPENILLVSSENVKLPDNKRSAANETHFRCLPKSSAIKLI 240

Query: 241 DFGSTAFDNENHSSIVSTRHYRAPEIILGLGWSYPCDLWSIGCILVELCSGKALFQTHEN 300
           DFGST  DN  H SIV TRHYR+PE+ILGLGWSY CDLWSIGCIL ELC+G+ALFQTH+N
Sbjct: 241 DFGSTVCDNRIHHSIVQTRHYRSPEVILGLGWSYQCDLWSIGCILFELCTGEALFQTHDN 300

Query: 301 LEHLAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLDRLKDM 360
           LEHLAMMER LGPLP HM + A + AEKYF+RG RLNWPEGA SRESIRAVK+LDRLKDM
Sbjct: 301 LEHLAMMERALGPLPEHMTRKASRGAEKYFRRGCRLNWPEGANSRESIRAVKRLDRLKDM 360

Query: 361 VSQHLGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKS 402
           VS+H+  +RS   DLLY LL YDPSER TA +AL+HPFFKS
Sbjct: 361 VSKHVDNTRSRFADLLYGLLAYDPSERLTANEALDHPFFKS 398

[Close]
BLAST of MC08g0325 vs. ExPASy Swiss-Prot
Match: P51566 (Serine/threonine-protein kinase AFC1 OS=Arabidopsis thaliana OX=3702 GN=AFC1 PE=2 SV=2)

HSP 1 Score: 543.5 bits (1399), Expect = 2.0e-153
Identity = 265/409 (64.79%), Postives = 318/409 (77.75%), Query Frame = 0 

Query: 18  RKRPRSAWDVVPS-----------------EQEAHKALVAG-------KDEVKRHASPPR 77
           RKRPR  WD  P                    E    LV          + + R  SPP 
Sbjct: 35  RKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNFVYPNMFYNGLPRQGSPPW 94

Query: 78  RDDDREGHYVFNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRD 137
           R DD++GHYVF +G+ LTPRY+ILSKMGEGTFG+VLEC+D + +E VAIKV+RSI KYR+
Sbjct: 95  RPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDNKNKEVVAIKVIRSINKYRE 154

Query: 138 AAMVEVDILKHLAQNDKGSLRCVQIRNWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPV 197
           AAM+E+D+L+ L ++D G  RCVQIRNWFDYRNHICIVFEKLGPSL+DFL++N Y  FP+
Sbjct: 155 AAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNSYRSFPI 214

Query: 198 DLVREFGRQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKKVS---SDETQFRC 257
           DLVRE GRQLLESVAYMHDL LIHTDLKPENILLVSSEYIK+P  K +S    D + F+ 
Sbjct: 215 DLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYIKIPDYKFLSRPTKDGSYFKN 274

Query: 258 LPKSSAIKLIDFGSTAFDNENHSSIVSTRHYRAPEIILGLGWSYPCDLWSIGCILVELCS 317
           LPKSSAIKLIDFGST F++++H+ IVSTRHYRAPE+ILG+GW+YPCDLWSIGCILVELCS
Sbjct: 275 LPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVGWNYPCDLWSIGCILVELCS 334

Query: 318 GKALFQTHENLEHLAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRA 377
           G+ALFQTHENLEHLAMMERVLGPLP HM+  AD+ +EKYF+RG++L+WPEGA SR+S++A
Sbjct: 335 GEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFRRGAKLDWPEGATSRDSLKA 394

Query: 378 VKKLDRLKDMVSQHLGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFF 400
           V KL RL +++ QH+  S   L DLL  LL+YDP+ER  AR+ALNHPFF
Sbjct: 395 VWKLPRLPNLIMQHVDHSAGDLIDLLQGLLRYDPTERFKAREALNHPFF 443

[Close]
BLAST of MC08g0325 vs. ExPASy Swiss-Prot
Match: P51567 (Serine/threonine-protein kinase AFC2 OS=Arabidopsis thaliana OX=3702 GN=AFC2 PE=1 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 1.2e-145
Identity = 245/424 (57.78%), Postives = 322/424 (75.94%), Query Frame = 0 

Query: 4   MEADRMEK----EQQRTTRKRPRSAWDVVPSEQEAHKALVAGKD---------------- 63
           ME +R+ +       R  RKR R  WDV+P   +A   +  G++                
Sbjct: 1   MEMERVHEFPHTHMDRRPRKRARLGWDVLPQATKAQVGMFCGQEIGNISSFASSGAPSDN 60

Query: 64  -------EVKRHASPPRRDDDREGHYVFNLGENLTPRYKILSKMGEGTFGRVLECWDRQT 123
                   V R+ SPP R+DD++GHY+F LG++LTPRYKI SKMGEGTFG+VLECWDR+ 
Sbjct: 61  SSSLCVKGVARNGSPPWREDDKDGHYIFELGDDLTPRYKIYSKMGEGTFGQVLECWDRER 120

Query: 124 REYVAIKVVRSIRKYRDAAMVEVDILKHLAQNDKGSLRCVQIRNWFDYRNHICIVFEKLG 183
           +E VA+K+VR ++KYR+AAM+E+++L+ L ++DKG  RCVQIRNWFDYRNHICIVFEKLG
Sbjct: 121 KEMVAVKIVRGVKKYREAAMIEIEMLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKLG 180

Query: 184 PSLFDFLKRNKYCPFPVDLVREFGRQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKLP 243
            SL+DFL++N Y  FP+DLVRE G QLLE VA+MHDL +IHTDLKPENILLVSS+Y+K+P
Sbjct: 181 SSLYDFLRKNNYRSFPIDLVREIGWQLLECVAFMHDLRMIHTDLKPENILLVSSDYVKIP 240

Query: 244 GCK-KVSSDETQFRCLPKSSAIKLIDFGSTAFDNENHSSIVSTRHYRAPEIILGLGWSYP 303
             K      +  ++ +PKSSAIK+IDFGST ++ ++ + IVSTRHYRAPE+ILGLGWSYP
Sbjct: 241 EYKGSRLQRDVCYKRVPKSSAIKVIDFGSTTYERQDQTYIVSTRHYRAPEVILGLGWSYP 300

Query: 304 CDLWSIGCILVELCSGKALFQTHENLEHLAMMERVLGPLPGHMIQSADQNAEKYFKRGSR 363
           CD+WS+GCI+VELC+G+ALFQTHENLEHLAMMERVLGP P  M++  D+++EKY +RG R
Sbjct: 301 CDVWSVGCIIVELCTGEALFQTHENLEHLAMMERVLGPFPQQMLKKVDRHSEKYVRRG-R 360

Query: 364 LNWPEGAVSRESIRAVKKLDRLKDMVSQHLGFSRSLLTDLLYDLLKYDPSERPTARQALN 400
           L+WP+GA SR+S++AV KL RL++++ QH+  S   L +++  LL++DPSER TAR+AL 
Sbjct: 361 LDWPDGATSRDSLKAVLKLPRLQNLIMQHVDHSAGELINMVQGLLRFDPSERITAREALR 420

[Close]
BLAST of MC08g0325 vs. ExPASy Swiss-Prot
Match: Q10156 (Dual specificity protein kinase lkh1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=lkh1 PE=1 SV=3)

HSP 1 Score: 344.0 bits (881), Expect = 2.3e-93
Identity = 176/388 (45.36%), Postives = 252/388 (64.95%), Query Frame = 0 

Query: 15  RTTRKRPRSAWDVVPSEQEAHKALVAGKDEVKRHASPPRRDDDREGHYVFNLGENLTPRY 74
           R  +KR R+AW   P  ++  +  V    +    A  P   DD +GHY          RY
Sbjct: 308 RPPKKRKRAAW---PPYKKVDRVNVPVVHDTT--AFDPSTFDDDDGHYKVVPNSKFANRY 367

Query: 75  KILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILKHLAQND-KGSL 134
            ++  +G GTFG+V++C+D+ T  + AIKV R+I KYR+A+++E+ +L+ +A +D     
Sbjct: 368 TVVRLLGHGTFGKVIQCYDQSTGRHCAIKVTRAIPKYREASLIELRVLQTIAHSDPTNEN 427

Query: 135 RCVQIRNWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFGRQLLESVAYMHDL 194
           +C+Q+R++FDYR HICIV +  G S+FDFLK N Y PFP+  ++   +QL +SVA++H L
Sbjct: 428 KCIQLRDYFDYRKHICIVTDLFGWSVFDFLKNNNYIPFPLKHIQMLSQQLFKSVAFLHSL 487

Query: 195 HLIHTDLKPENILLVS--SEYIKLPGCKKVSSDETQFRCLPKSSAIKLIDFGSTAFDNEN 254
            L+HTDLKPEN+LLVS  S  I+LP            + +  S  I+LIDFGS  F++E 
Sbjct: 488 GLVHTDLKPENVLLVSNASRTIRLP-------YRNYSQKVLNSCEIRLIDFGSATFEDEY 547

Query: 255 HSSIVSTRHYRAPEIILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEHLAMMERVL 314
           HSS+VSTRHYRAPEIILGLGWSYPCD+WSIGCILVEL +G+ALFQTHE+ EHL MME++L
Sbjct: 548 HSSVVSTRHYRAPEIILGLGWSYPCDVWSIGCILVELFTGQALFQTHEDSEHLCMMEKIL 607

Query: 315 GPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLDRLKDMVSQHLGFSRSL 374
           GP   +MI  + + ++++FK   ++ +P     ++SI  ++ L  L+ + +       +L
Sbjct: 608 GPFDRNMISRSSRTSQRFFKSDGKVRYPLSNTPKKSINYLQSLQTLEQIFAVS-SPEVAL 667

Query: 375 LTDLLYDLLKYDPSERPTARQALNHPFF 400
           L DLL  +  YDP  R TA++AL HPFF
Sbjct: 668 LLDLLKKVFVYDPKRRITAKEALWHPFF 682

[Close]
BLAST of MC08g0325 vs. ExPASy Swiss-Prot
Match: P49760 (Dual specificity protein kinase CLK2 OS=Homo sapiens OX=9606 GN=CLK2 PE=1 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 1.9e-90
Identity = 166/351 (47.29%), Postives = 238/351 (67.81%), Query Frame = 0 

Query: 55  DDDREGHYVFNLGENLTPRYKILSKMGEGTFGRVLECWD-RQTREYVAIKVVRSIRKYRD 114
           +DD EGH ++++G+ L  RY+I+S +GEGTFGRV++C D R+    VA+K+++++ KY++
Sbjct: 144 EDDAEGHLIYHVGDWLQERYEIVSTLGEGTFGRVVQCVDHRRGGARVALKIIKNVEKYKE 203

Query: 115 AAMVEVDILKHLAQNDKGSLR-CVQIRNWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFP 174
           AA +E+++L+ + + D  +   CVQ+ +WFDY  H+CI FE LG S FDFLK N Y P+P
Sbjct: 204 AARLEINVLEKINEKDPDNKNLCVQMFDWFDYHGHMCISFELLGLSTFDFLKDNNYLPYP 263

Query: 175 VDLVREFGRQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKKVSSDETQFRCLP 234
           +  VR    QL ++V ++HD  L HTDLKPENIL V+S+Y      +K   + +      
Sbjct: 264 IHQVRHMAFQLCQAVKFLHDNKLTHTDLKPENILFVNSDYELTYNLEKKRDERS-----V 323

Query: 235 KSSAIKLIDFGSTAFDNENHSSIVSTRHYRAPEIILGLGWSYPCDLWSIGCILVELCSGK 294
           KS+A++++DFGS  FD+E+HS+IVSTRHYRAPE+IL LGWS PCD+WSIGCI+ E   G 
Sbjct: 324 KSTAVRVVDFGSATFDHEHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGCIIFEYYVGF 383

Query: 295 ALFQTHENLEHLAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPE----GAVSRESI 354
            LFQTH+N EHLAMMER+LGP+P  MI+      +KYF RG RL+W E    G   RE+ 
Sbjct: 384 TLFQTHDNREHLAMMERILGPIPSRMIRKT--RKQKYFYRG-RLDWDENTSAGRYVRENC 443

Query: 355 RAVKKLDRLKDMVSQHLGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFF 400
           + +++   L     +H       L DL+  +L+Y+P++R T  +AL HPFF
Sbjct: 444 KPLRRY--LTSEAEEH-----HQLFDLIESMLEYEPAKRLTLGEALQHPFF 479

[Close]
BLAST of MC08g0325 vs. NCBI nr
Match: XP_022132125.1 (serine/threonine-protein kinase AFC3 [Momordica charantia])

HSP 1 Score: 826 bits (2134), Expect = 2.21e-302
Identity = 402/403 (99.75%), Postives = 402/403 (99.75%), Query Frame = 0 

Query: 1   MVAMEADRMEKEQQRTTRKRPRSAWDVVPSEQEAHKALVAGKDEVKRHASPPRRDDDREG 60
           MVAMEADRMEKEQQRTTRKRPRSAWDVVPSEQEAHKALV GKDEVKRHASPPRRDDDREG
Sbjct: 1   MVAMEADRMEKEQQRTTRKRPRSAWDVVPSEQEAHKALVVGKDEVKRHASPPRRDDDREG 60

Query: 61  HYVFNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVD 120
           HYVFNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVD
Sbjct: 61  HYVFNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVD 120

Query: 121 ILKHLAQNDKGSLRCVQIRNWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFG 180
           ILKHLAQNDKGSLRCVQIRNWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFG
Sbjct: 121 ILKHLAQNDKGSLRCVQIRNWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFG 180

Query: 181 RQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKKVSSDETQFRCLPKSSAIKLI 240
           RQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKKVSSDETQFRCLPKSSAIKLI
Sbjct: 181 RQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKKVSSDETQFRCLPKSSAIKLI 240

Query: 241 DFGSTAFDNENHSSIVSTRHYRAPEIILGLGWSYPCDLWSIGCILVELCSGKALFQTHEN 300
           DFGSTAFDNENHSSIVSTRHYRAPEIILGLGWSYPCDLWSIGCILVELCSGKALFQTHEN
Sbjct: 241 DFGSTAFDNENHSSIVSTRHYRAPEIILGLGWSYPCDLWSIGCILVELCSGKALFQTHEN 300

Query: 301 LEHLAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLDRLKDM 360
           LEHLAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLDRLKDM
Sbjct: 301 LEHLAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLDRLKDM 360

Query: 361 VSQHLGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA 403
           VSQHLGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA
Sbjct: 361 VSQHLGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA 403

[Close]
BLAST of MC08g0325 vs. NCBI nr
Match: XP_038886114.1 (serine/threonine-protein kinase AFC3 [Benincasa hispida])

HSP 1 Score: 794 bits (2051), Expect = 8.87e-290
Identity = 385/400 (96.25%), Postives = 394/400 (98.50%), Query Frame = 0 

Query: 4   MEADRMEKEQQRTTRKRPRSAWDVVPSEQEAHKALVAGKDEVKRHASPPRRDDDREGHYV 63
           MEA+RM KEQQRTTRKRPRSAWDVVPSEQEA+KALV  K++VKRHASPPRRDDDREGHYV
Sbjct: 1   MEAERMGKEQQRTTRKRPRSAWDVVPSEQEANKALVVVKNDVKRHASPPRRDDDREGHYV 60

Query: 64  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 123
           FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK
Sbjct: 61  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 120

Query: 124 HLAQNDKGSLRCVQIRNWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFGRQL 183
           HLAQND GSLRCVQIR WFDYRNHICIVFEKLGPSLFDFLKRN+YCPFPVDLVREFGRQL
Sbjct: 121 HLAQNDTGSLRCVQIRTWFDYRNHICIVFEKLGPSLFDFLKRNRYCPFPVDLVREFGRQL 180

Query: 184 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKKVSSDETQFRCLPKSSAIKLIDFG 243
           LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCK+VSSDETQFRCLPKSSAIKLIDFG
Sbjct: 181 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG 240

Query: 244 STAFDNENHSSIVSTRHYRAPEIILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 303
           STAFDNENHSSIVSTRHYRAPE+ILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH
Sbjct: 241 STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 300

Query: 304 LAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 363
           LAMMERVLGPLPGHMIQSADQNAEKYFKRG RLNWPEGAVSRESIRAVKKLDRLKDMVSQ
Sbjct: 301 LAMMERVLGPLPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 360

Query: 364 HLGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA 403
           ++GFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA
Sbjct: 361 YVGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA 400

[Close]
BLAST of MC08g0325 vs. NCBI nr
Match: XP_008445045.1 (PREDICTED: serine/threonine-protein kinase AFC3 [Cucumis melo])

HSP 1 Score: 792 bits (2045), Expect = 7.29e-289
Identity = 384/400 (96.00%), Postives = 392/400 (98.00%), Query Frame = 0 

Query: 4   MEADRMEKEQQRTTRKRPRSAWDVVPSEQEAHKALVAGKDEVKRHASPPRRDDDREGHYV 63
           MEADRMEKEQQR TRKRPRSAWDVVP  QEA+KAL   K+EVKRHASPPRRDDDREGHYV
Sbjct: 1   MEADRMEKEQQRITRKRPRSAWDVVPPAQEANKALAVVKNEVKRHASPPRRDDDREGHYV 60

Query: 64  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 123
           FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK
Sbjct: 61  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 120

Query: 124 HLAQNDKGSLRCVQIRNWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFGRQL 183
           HLAQN+ GSLRCVQIR WFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFGRQL
Sbjct: 121 HLAQNEMGSLRCVQIRTWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFGRQL 180

Query: 184 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKKVSSDETQFRCLPKSSAIKLIDFG 243
           LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCK+VSSDETQFRCLPKSSAIKLIDFG
Sbjct: 181 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG 240

Query: 244 STAFDNENHSSIVSTRHYRAPEIILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 303
           STAFDNENHSSIVSTRHYRAPE+ILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH
Sbjct: 241 STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 300

Query: 304 LAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 363
           LAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLDRLKDMVSQ
Sbjct: 301 LAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 360

Query: 364 HLGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA 403
           ++GFSRSLLTDLLYDLLKYDPSERPTAR+ALNHPFFKSIA
Sbjct: 361 YVGFSRSLLTDLLYDLLKYDPSERPTARRALNHPFFKSIA 400

[Close]
BLAST of MC08g0325 vs. NCBI nr
Match: XP_022996479.1 (serine/threonine-protein kinase AFC3 isoform X1 [Cucurbita maxima])

HSP 1 Score: 789 bits (2038), Expect = 9.17e-288
Identity = 386/403 (95.78%), Postives = 394/403 (97.77%), Query Frame = 0 

Query: 1   MVAMEADRMEKEQQRTTRKRPRSAWDVVPSEQEAHKALVAGKDEVKRHASPPRRDDDREG 60
           M AMEA+RMEKEQQRTTRKRPRSAWDVVPSEQEA+KALV  KDEVKRHASPPRRDDDREG
Sbjct: 1   MSAMEAERMEKEQQRTTRKRPRSAWDVVPSEQEANKALVVVKDEVKRHASPPRRDDDREG 60

Query: 61  HYVFNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVD 120
           HYVFNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVD
Sbjct: 61  HYVFNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVD 120

Query: 121 ILKHLAQNDKGSLRCVQIRNWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFG 180
           ILKHLAQND G LRCVQIRNWFDYRNHICIVFE+LGPSLFDFLKRNKY PFPVDLVREFG
Sbjct: 121 ILKHLAQNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFG 180

Query: 181 RQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKKVSSDETQFRCLPKSSAIKLI 240
           RQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCK+VSSDETQFRCLPKSSAIKLI
Sbjct: 181 RQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLI 240

Query: 241 DFGSTAFDNENHSSIVSTRHYRAPEIILGLGWSYPCDLWSIGCILVELCSGKALFQTHEN 300
           DFGSTAFDNENHSSIVSTRHYRAPE+ILGLGWSYPCDLWSIGCILVELCSGKALFQTHEN
Sbjct: 241 DFGSTAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHEN 300

Query: 301 LEHLAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLDRLKDM 360
           LEHLAMMERVLGP+PGHMIQSADQNAEKYFKRG RLNWPEGAVSRESIRAVKKLDRLKDM
Sbjct: 301 LEHLAMMERVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDM 360

Query: 361 VSQHLGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA 403
           VSQH+GFSR LLTDLLYDLLKYDPSERPTARQAL+H FFKSIA
Sbjct: 361 VSQHVGFSR-LLTDLLYDLLKYDPSERPTARQALDHSFFKSIA 402

[Close]
BLAST of MC08g0325 vs. NCBI nr
Match: XP_004138782.1 (serine/threonine-protein kinase AFC3 [Cucumis sativus] >KGN62838.1 hypothetical protein Csa_022298 [Cucumis sativus])

HSP 1 Score: 787 bits (2033), Expect = 4.92e-287
Identity = 382/400 (95.50%), Postives = 390/400 (97.50%), Query Frame = 0 

Query: 4   MEADRMEKEQQRTTRKRPRSAWDVVPSEQEAHKALVAGKDEVKRHASPPRRDDDREGHYV 63
           MEADRMEKEQQRT+RKRPRSAWDVVP  QEA+KAL   K+EVKRH SPPRRDDDREGHYV
Sbjct: 1   MEADRMEKEQQRTSRKRPRSAWDVVPPAQEANKALAVVKNEVKRHVSPPRRDDDREGHYV 60

Query: 64  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 123
           FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK
Sbjct: 61  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 120

Query: 124 HLAQNDKGSLRCVQIRNWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFGRQL 183
           HLAQN+ GSL CVQIR WFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFGRQL
Sbjct: 121 HLAQNEMGSLCCVQIRTWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFGRQL 180

Query: 184 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKKVSSDETQFRCLPKSSAIKLIDFG 243
           LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCK+VSSDETQFRCLPKSSAIKLIDFG
Sbjct: 181 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG 240

Query: 244 STAFDNENHSSIVSTRHYRAPEIILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 303
           STAFDNENHSSIVSTRHYRAPE+ILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH
Sbjct: 241 STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 300

Query: 304 LAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 363
           LAMMERVLGPLPGHMIQSADQNAEKYFKRG RLNWPEGAVSRESIRAVKKLDRLKDMVSQ
Sbjct: 301 LAMMERVLGPLPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 360

Query: 364 HLGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA 403
           ++GFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA
Sbjct: 361 YVGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA 400

[Close]
BLAST of MC08g0325 vs. ExPASy TrEMBL
Match: A0A6J1BRK0 (serine/threonine-protein kinase AFC3 OS=Momordica charantia OX=3673 GN=LOC111005069 PE=4 SV=1)

HSP 1 Score: 826 bits (2134), Expect = 1.07e-302
Identity = 402/403 (99.75%), Postives = 402/403 (99.75%), Query Frame = 0 

Query: 1   MVAMEADRMEKEQQRTTRKRPRSAWDVVPSEQEAHKALVAGKDEVKRHASPPRRDDDREG 60
           MVAMEADRMEKEQQRTTRKRPRSAWDVVPSEQEAHKALV GKDEVKRHASPPRRDDDREG
Sbjct: 1   MVAMEADRMEKEQQRTTRKRPRSAWDVVPSEQEAHKALVVGKDEVKRHASPPRRDDDREG 60

Query: 61  HYVFNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVD 120
           HYVFNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVD
Sbjct: 61  HYVFNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVD 120

Query: 121 ILKHLAQNDKGSLRCVQIRNWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFG 180
           ILKHLAQNDKGSLRCVQIRNWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFG
Sbjct: 121 ILKHLAQNDKGSLRCVQIRNWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFG 180

Query: 181 RQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKKVSSDETQFRCLPKSSAIKLI 240
           RQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKKVSSDETQFRCLPKSSAIKLI
Sbjct: 181 RQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKKVSSDETQFRCLPKSSAIKLI 240

Query: 241 DFGSTAFDNENHSSIVSTRHYRAPEIILGLGWSYPCDLWSIGCILVELCSGKALFQTHEN 300
           DFGSTAFDNENHSSIVSTRHYRAPEIILGLGWSYPCDLWSIGCILVELCSGKALFQTHEN
Sbjct: 241 DFGSTAFDNENHSSIVSTRHYRAPEIILGLGWSYPCDLWSIGCILVELCSGKALFQTHEN 300

Query: 301 LEHLAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLDRLKDM 360
           LEHLAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLDRLKDM
Sbjct: 301 LEHLAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLDRLKDM 360

Query: 361 VSQHLGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA 403
           VSQHLGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA
Sbjct: 361 VSQHLGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA 403

[Close]
BLAST of MC08g0325 vs. ExPASy TrEMBL
Match: A0A1S3BCM1 (serine/threonine-protein kinase AFC3 OS=Cucumis melo OX=3656 GN=LOC103488204 PE=4 SV=1)

HSP 1 Score: 792 bits (2045), Expect = 3.53e-289
Identity = 384/400 (96.00%), Postives = 392/400 (98.00%), Query Frame = 0 

Query: 4   MEADRMEKEQQRTTRKRPRSAWDVVPSEQEAHKALVAGKDEVKRHASPPRRDDDREGHYV 63
           MEADRMEKEQQR TRKRPRSAWDVVP  QEA+KAL   K+EVKRHASPPRRDDDREGHYV
Sbjct: 1   MEADRMEKEQQRITRKRPRSAWDVVPPAQEANKALAVVKNEVKRHASPPRRDDDREGHYV 60

Query: 64  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 123
           FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK
Sbjct: 61  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 120

Query: 124 HLAQNDKGSLRCVQIRNWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFGRQL 183
           HLAQN+ GSLRCVQIR WFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFGRQL
Sbjct: 121 HLAQNEMGSLRCVQIRTWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFGRQL 180

Query: 184 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKKVSSDETQFRCLPKSSAIKLIDFG 243
           LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCK+VSSDETQFRCLPKSSAIKLIDFG
Sbjct: 181 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG 240

Query: 244 STAFDNENHSSIVSTRHYRAPEIILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 303
           STAFDNENHSSIVSTRHYRAPE+ILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH
Sbjct: 241 STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 300

Query: 304 LAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 363
           LAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLDRLKDMVSQ
Sbjct: 301 LAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 360

Query: 364 HLGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA 403
           ++GFSRSLLTDLLYDLLKYDPSERPTAR+ALNHPFFKSIA
Sbjct: 361 YVGFSRSLLTDLLYDLLKYDPSERPTARRALNHPFFKSIA 400

[Close]
BLAST of MC08g0325 vs. ExPASy TrEMBL
Match: A0A6J1K8U5 (serine/threonine-protein kinase AFC3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491714 PE=4 SV=1)

HSP 1 Score: 789 bits (2038), Expect = 4.44e-288
Identity = 386/403 (95.78%), Postives = 394/403 (97.77%), Query Frame = 0 

Query: 1   MVAMEADRMEKEQQRTTRKRPRSAWDVVPSEQEAHKALVAGKDEVKRHASPPRRDDDREG 60
           M AMEA+RMEKEQQRTTRKRPRSAWDVVPSEQEA+KALV  KDEVKRHASPPRRDDDREG
Sbjct: 1   MSAMEAERMEKEQQRTTRKRPRSAWDVVPSEQEANKALVVVKDEVKRHASPPRRDDDREG 60

Query: 61  HYVFNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVD 120
           HYVFNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVD
Sbjct: 61  HYVFNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVD 120

Query: 121 ILKHLAQNDKGSLRCVQIRNWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFG 180
           ILKHLAQND G LRCVQIRNWFDYRNHICIVFE+LGPSLFDFLKRNKY PFPVDLVREFG
Sbjct: 121 ILKHLAQNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFG 180

Query: 181 RQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKKVSSDETQFRCLPKSSAIKLI 240
           RQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCK+VSSDETQFRCLPKSSAIKLI
Sbjct: 181 RQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLI 240

Query: 241 DFGSTAFDNENHSSIVSTRHYRAPEIILGLGWSYPCDLWSIGCILVELCSGKALFQTHEN 300
           DFGSTAFDNENHSSIVSTRHYRAPE+ILGLGWSYPCDLWSIGCILVELCSGKALFQTHEN
Sbjct: 241 DFGSTAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHEN 300

Query: 301 LEHLAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLDRLKDM 360
           LEHLAMMERVLGP+PGHMIQSADQNAEKYFKRG RLNWPEGAVSRESIRAVKKLDRLKDM
Sbjct: 301 LEHLAMMERVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDM 360

Query: 361 VSQHLGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA 403
           VSQH+GFSR LLTDLLYDLLKYDPSERPTARQAL+H FFKSIA
Sbjct: 361 VSQHVGFSR-LLTDLLYDLLKYDPSERPTARQALDHSFFKSIA 402

[Close]
BLAST of MC08g0325 vs. ExPASy TrEMBL
Match: A0A0A0LM05 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G375780 PE=4 SV=1)

HSP 1 Score: 787 bits (2033), Expect = 2.38e-287
Identity = 382/400 (95.50%), Postives = 390/400 (97.50%), Query Frame = 0 

Query: 4   MEADRMEKEQQRTTRKRPRSAWDVVPSEQEAHKALVAGKDEVKRHASPPRRDDDREGHYV 63
           MEADRMEKEQQRT+RKRPRSAWDVVP  QEA+KAL   K+EVKRH SPPRRDDDREGHYV
Sbjct: 1   MEADRMEKEQQRTSRKRPRSAWDVVPPAQEANKALAVVKNEVKRHVSPPRRDDDREGHYV 60

Query: 64  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 123
           FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK
Sbjct: 61  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 120

Query: 124 HLAQNDKGSLRCVQIRNWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFGRQL 183
           HLAQN+ GSL CVQIR WFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFGRQL
Sbjct: 121 HLAQNEMGSLCCVQIRTWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFGRQL 180

Query: 184 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKKVSSDETQFRCLPKSSAIKLIDFG 243
           LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCK+VSSDETQFRCLPKSSAIKLIDFG
Sbjct: 181 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG 240

Query: 244 STAFDNENHSSIVSTRHYRAPEIILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 303
           STAFDNENHSSIVSTRHYRAPE+ILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH
Sbjct: 241 STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 300

Query: 304 LAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 363
           LAMMERVLGPLPGHMIQSADQNAEKYFKRG RLNWPEGAVSRESIRAVKKLDRLKDMVSQ
Sbjct: 301 LAMMERVLGPLPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 360

Query: 364 HLGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA 403
           ++GFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA
Sbjct: 361 YVGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA 400

[Close]
BLAST of MC08g0325 vs. ExPASy TrEMBL
Match: A0A5A7V9M0 (Serine/threonine-protein kinase AFC3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G003470 PE=4 SV=1)

HSP 1 Score: 788 bits (2035), Expect = 8.08e-287
Identity = 386/408 (94.61%), Postives = 393/408 (96.32%), Query Frame = 0 

Query: 3   AMEADRMEKEQQRTTRKRPRSAWDVVPSEQEAHKALVAGKDEVKRHASPPRRDDDREGHY 62
           AMEADRMEKEQQR TRKRPRSAWDVVP  QEA+KAL   K+EVKRHASPPRRDDDREGHY
Sbjct: 44  AMEADRMEKEQQRITRKRPRSAWDVVPPAQEANKALAVVKNEVKRHASPPRRDDDREGHY 103

Query: 63  VFNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDIL 122
           VFNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDIL
Sbjct: 104 VFNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDIL 163

Query: 123 KHLAQNDKGSLRCVQIRNWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFGRQ 182
           KHLAQN+ GSLRCVQIR WFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFGRQ
Sbjct: 164 KHLAQNEMGSLRCVQIRTWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFGRQ 223

Query: 183 LLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKK-------VSSDETQFRCLPKSS 242
           LLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCK+       VSSDETQFRCLPKSS
Sbjct: 224 LLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRMLEMIQRVSSDETQFRCLPKSS 283

Query: 243 AIKLIDFGSTAFDNENHSSIVSTRHYRAPEIILGLGWSYPCDLWSIGCILVELCSGKALF 302
           AIKLIDFGSTAFDNENHSSIVSTRHYRAPE+ILGLGWSYPCDLWSIGCILVELCSGKALF
Sbjct: 284 AIKLIDFGSTAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALF 343

Query: 303 QTHENLEHLAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLD 362
           QTHENLEHLAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLD
Sbjct: 344 QTHENLEHLAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLD 403

Query: 363 RLKDMVSQHLGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA 403
           RLKDMVSQ++GFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA
Sbjct: 404 RLKDMVSQYVGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA 451

[Close]
BLAST of MC08g0325 vs. TAIR 10
Match: AT4G32660.1 (Protein kinase superfamily protein )

HSP 1 Score: 611.3 bits (1575), Expect = 5.6e-175
Identity = 291/401 (72.57%), Postives = 337/401 (84.04%), Query Frame = 0 

Query: 1   MVAMEADRMEKEQQRTTRKRPRSAWDVVPSEQEAHKALVAGKDEVKRHASPPRRDDDREG 60
           M+A   + M+KE+    RKRPR  WD  P+E EA +A++ G     R  SPP RDDDR+G
Sbjct: 1   MIANGFESMDKER---VRKRPRMTWDEAPAEPEAKRAVIKGHGSDGRILSPPLRDDDRDG 60

Query: 61  HYVFNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVD 120
           HYVF+L +NLTPRYKILSKMGEGTFGRVLECWDR T+EYVAIK++RSI+KYRDAAM+E+D
Sbjct: 61  HYVFSLRDNLTPRYKILSKMGEGTFGRVLECWDRDTKEYVAIKIIRSIKKYRDAAMIEID 120

Query: 121 ILKHLAQNDKGSLRCVQIRNWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFG 180
           +L+ L ++DKG  RCVQ++NWFDYRNHICIVFEKLGPSLFDFLKRNKY  FP+ LVR+FG
Sbjct: 121 VLQKLVKSDKGRTRCVQMKNWFDYRNHICIVFEKLGPSLFDFLKRNKYSAFPLALVRDFG 180

Query: 181 RQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKKVSSDETQFRCLPKSSAIKLI 240
            QLLESVAYMH+L L+HTDLKPENILLVSSE +KLP  K+ +++ET FRCLPKSSAIKLI
Sbjct: 181 CQLLESVAYMHELQLVHTDLKPENILLVSSENVKLPDNKRSAANETHFRCLPKSSAIKLI 240

Query: 241 DFGSTAFDNENHSSIVSTRHYRAPEIILGLGWSYPCDLWSIGCILVELCSGKALFQTHEN 300
           DFGST  DN  H SIV TRHYR+PE+ILGLGWSY CDLWSIGCIL ELC+G+ALFQTH+N
Sbjct: 241 DFGSTVCDNRIHHSIVQTRHYRSPEVILGLGWSYQCDLWSIGCILFELCTGEALFQTHDN 300

Query: 301 LEHLAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLDRLKDM 360
           LEHLAMMER LGPLP HM + A + AEKYF+RG RLNWPEGA SRESIRAVK+LDRLKDM
Sbjct: 301 LEHLAMMERALGPLPEHMTRKASRGAEKYFRRGCRLNWPEGANSRESIRAVKRLDRLKDM 360

Query: 361 VSQHLGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKS 402
           VS+H+  +RS   DLLY LL YDPSER TA +AL+HPFFKS
Sbjct: 361 VSKHVDNTRSRFADLLYGLLAYDPSERLTANEALDHPFFKS 398

[Close]
BLAST of MC08g0325 vs. TAIR 10
Match: AT4G32660.3 (Protein kinase superfamily protein )

HSP 1 Score: 609.0 bits (1569), Expect = 2.8e-174
Identity = 289/393 (73.54%), Postives = 333/393 (84.73%), Query Frame = 0 

Query: 9   MEKEQQRTTRKRPRSAWDVVPSEQEAHKALVAGKDEVKRHASPPRRDDDREGHYVFNLGE 68
           M+KE+    RKRPR  WD  P+E EA +A++ G     R  SPP RDDDR+GHYVF+L +
Sbjct: 1   MDKER---VRKRPRMTWDEAPAEPEAKRAVIKGHGSDGRILSPPLRDDDRDGHYVFSLRD 60

Query: 69  NLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILKHLAQN 128
           NLTPRYKILSKMGEGTFGRVLECWDR T+EYVAIK++RSI+KYRDAAM+E+D+L+ L ++
Sbjct: 61  NLTPRYKILSKMGEGTFGRVLECWDRDTKEYVAIKIIRSIKKYRDAAMIEIDVLQKLVKS 120

Query: 129 DKGSLRCVQIRNWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFGRQLLESVA 188
           DKG  RCVQ++NWFDYRNHICIVFEKLGPSLFDFLKRNKY  FP+ LVR+FG QLLESVA
Sbjct: 121 DKGRTRCVQMKNWFDYRNHICIVFEKLGPSLFDFLKRNKYSAFPLALVRDFGCQLLESVA 180

Query: 189 YMHDLHLIHTDLKPENILLVSSEYIKLPGCKKVSSDETQFRCLPKSSAIKLIDFGSTAFD 248
           YMH+L L+HTDLKPENILLVSSE +KLP  K+ +++ET FRCLPKSSAIKLIDFGST  D
Sbjct: 181 YMHELQLVHTDLKPENILLVSSENVKLPDNKRSAANETHFRCLPKSSAIKLIDFGSTVCD 240

Query: 249 NENHSSIVSTRHYRAPEIILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEHLAMME 308
           N  H SIV TRHYR+PE+ILGLGWSY CDLWSIGCIL ELC+G+ALFQTH+NLEHLAMME
Sbjct: 241 NRIHHSIVQTRHYRSPEVILGLGWSYQCDLWSIGCILFELCTGEALFQTHDNLEHLAMME 300

Query: 309 RVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLDRLKDMVSQHLGFS 368
           R LGPLP HM + A + AEKYF+RG RLNWPEGA SRESIRAVK+LDRLKDMVS+H+  +
Sbjct: 301 RALGPLPEHMTRKASRGAEKYFRRGCRLNWPEGANSRESIRAVKRLDRLKDMVSKHVDNT 360

Query: 369 RSLLTDLLYDLLKYDPSERPTARQALNHPFFKS 402
           RS   DLLY LL YDPSER TA +AL+HPFFKS
Sbjct: 361 RSRFADLLYGLLAYDPSERLTANEALDHPFFKS 390

[Close]
BLAST of MC08g0325 vs. TAIR 10
Match: AT4G32660.2 (Protein kinase superfamily protein )

HSP 1 Score: 553.5 bits (1425), Expect = 1.4e-157
Identity = 262/358 (73.18%), Postives = 303/358 (84.64%), Query Frame = 0 

Query: 1   MVAMEADRMEKEQQRTTRKRPRSAWDVVPSEQEAHKALVAGKDEVKRHASPPRRDDDREG 60
           M+A   + M+KE+    RKRPR  WD  P+E EA +A++ G     R  SPP RDDDR+G
Sbjct: 1   MIANGFESMDKER---VRKRPRMTWDEAPAEPEAKRAVIKGHGSDGRILSPPLRDDDRDG 60

Query: 61  HYVFNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVD 120
           HYVF+L +NLTPRYKILSKMGEGTFGRVLECWDR T+EYVAIK++RSI+KYRDAAM+E+D
Sbjct: 61  HYVFSLRDNLTPRYKILSKMGEGTFGRVLECWDRDTKEYVAIKIIRSIKKYRDAAMIEID 120

Query: 121 ILKHLAQNDKGSLRCVQIRNWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFG 180
           +L+ L ++DKG  RCVQ++NWFDYRNHICIVFEKLGPSLFDFLKRNKY  FP+ LVR+FG
Sbjct: 121 VLQKLVKSDKGRTRCVQMKNWFDYRNHICIVFEKLGPSLFDFLKRNKYSAFPLALVRDFG 180

Query: 181 RQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKKVSSDETQFRCLPKSSAIKLI 240
            QLLESVAYMH+L L+HTDLKPENILLVSSE +KLP  K+ +++ET FRCLPKSSAIKLI
Sbjct: 181 CQLLESVAYMHELQLVHTDLKPENILLVSSENVKLPDNKRSAANETHFRCLPKSSAIKLI 240

Query: 241 DFGSTAFDNENHSSIVSTRHYRAPEIILGLGWSYPCDLWSIGCILVELCSGKALFQTHEN 300
           DFGST  DN  H SIV TRHYR+PE+ILGLGWSY CDLWSIGCIL ELC+G+ALFQTH+N
Sbjct: 241 DFGSTVCDNRIHHSIVQTRHYRSPEVILGLGWSYQCDLWSIGCILFELCTGEALFQTHDN 300

Query: 301 LEHLAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLDRLK 359
           LEHLAMMER LGPLP HM + A + AEKYF+RG RLNWPEGA SRESIRAVK+LDRLK
Sbjct: 301 LEHLAMMERALGPLPEHMTRKASRGAEKYFRRGCRLNWPEGANSRESIRAVKRLDRLK 355

[Close]
BLAST of MC08g0325 vs. TAIR 10
Match: AT3G53570.3 (FUS3-complementing gene 1 )

HSP 1 Score: 543.5 bits (1399), Expect = 1.4e-154
Identity = 265/409 (64.79%), Postives = 318/409 (77.75%), Query Frame = 0 

Query: 18  RKRPRSAWDVVPS-----------------EQEAHKALVAG-------KDEVKRHASPPR 77
           RKRPR  WD  P                    E    LV          + + R  SPP 
Sbjct: 21  RKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNFVYPNMFYNGLPRQGSPPW 80

Query: 78  RDDDREGHYVFNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRD 137
           R DD++GHYVF +G+ LTPRY+ILSKMGEGTFG+VLEC+D + +E VAIKV+RSI KYR+
Sbjct: 81  RPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDNKNKEVVAIKVIRSINKYRE 140

Query: 138 AAMVEVDILKHLAQNDKGSLRCVQIRNWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPV 197
           AAM+E+D+L+ L ++D G  RCVQIRNWFDYRNHICIVFEKLGPSL+DFL++N Y  FP+
Sbjct: 141 AAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNSYRSFPI 200

Query: 198 DLVREFGRQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKKVS---SDETQFRC 257
           DLVRE GRQLLESVAYMHDL LIHTDLKPENILLVSSEYIK+P  K +S    D + F+ 
Sbjct: 201 DLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYIKIPDYKFLSRPTKDGSYFKN 260

Query: 258 LPKSSAIKLIDFGSTAFDNENHSSIVSTRHYRAPEIILGLGWSYPCDLWSIGCILVELCS 317
           LPKSSAIKLIDFGST F++++H+ IVSTRHYRAPE+ILG+GW+YPCDLWSIGCILVELCS
Sbjct: 261 LPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVGWNYPCDLWSIGCILVELCS 320

Query: 318 GKALFQTHENLEHLAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRA 377
           G+ALFQTHENLEHLAMMERVLGPLP HM+  AD+ +EKYF+RG++L+WPEGA SR+S++A
Sbjct: 321 GEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFRRGAKLDWPEGATSRDSLKA 380

Query: 378 VKKLDRLKDMVSQHLGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFF 400
           V KL RL +++ QH+  S   L DLL  LL+YDP+ER  AR+ALNHPFF
Sbjct: 381 VWKLPRLPNLIMQHVDHSAGDLIDLLQGLLRYDPTERFKAREALNHPFF 429

[Close]
BLAST of MC08g0325 vs. TAIR 10
Match: AT3G53570.1 (FUS3-complementing gene 1 )

HSP 1 Score: 543.5 bits (1399), Expect = 1.4e-154
Identity = 265/409 (64.79%), Postives = 318/409 (77.75%), Query Frame = 0 

Query: 18  RKRPRSAWDVVPS-----------------EQEAHKALVAG-------KDEVKRHASPPR 77
           RKRPR  WD  P                    E    LV          + + R  SPP 
Sbjct: 35  RKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNFVYPNMFYNGLPRQGSPPW 94

Query: 78  RDDDREGHYVFNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRD 137
           R DD++GHYVF +G+ LTPRY+ILSKMGEGTFG+VLEC+D + +E VAIKV+RSI KYR+
Sbjct: 95  RPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDNKNKEVVAIKVIRSINKYRE 154

Query: 138 AAMVEVDILKHLAQNDKGSLRCVQIRNWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPV 197
           AAM+E+D+L+ L ++D G  RCVQIRNWFDYRNHICIVFEKLGPSL+DFL++N Y  FP+
Sbjct: 155 AAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNSYRSFPI 214

Query: 198 DLVREFGRQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKKVS---SDETQFRC 257
           DLVRE GRQLLESVAYMHDL LIHTDLKPENILLVSSEYIK+P  K +S    D + F+ 
Sbjct: 215 DLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYIKIPDYKFLSRPTKDGSYFKN 274

Query: 258 LPKSSAIKLIDFGSTAFDNENHSSIVSTRHYRAPEIILGLGWSYPCDLWSIGCILVELCS 317
           LPKSSAIKLIDFGST F++++H+ IVSTRHYRAPE+ILG+GW+YPCDLWSIGCILVELCS
Sbjct: 275 LPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVGWNYPCDLWSIGCILVELCS 334

Query: 318 GKALFQTHENLEHLAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRA 377
           G+ALFQTHENLEHLAMMERVLGPLP HM+  AD+ +EKYF+RG++L+WPEGA SR+S++A
Sbjct: 335 GEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFRRGAKLDWPEGATSRDSLKA 394

Query: 378 VKKLDRLKDMVSQHLGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFF 400
           V KL RL +++ QH+  S   L DLL  LL+YDP+ER  AR+ALNHPFF
Sbjct: 395 VWKLPRLPNLIMQHVDHSAGDLIDLLQGLLRYDPTERFKAREALNHPFF 443

The following BLAST results are available for this feature:
BLAST of MC08g0325 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P515687.9e-17472.57Serine/threonine-protein kinase AFC3 OS=Arabidopsis thaliana OX=3702 GN=AFC3 PE=... [more]
P515662.0e-15364.79Serine/threonine-protein kinase AFC1 OS=Arabidopsis thaliana OX=3702 GN=AFC1 PE=... [more]
P515671.2e-14557.78Serine/threonine-protein kinase AFC2 OS=Arabidopsis thaliana OX=3702 GN=AFC2 PE=... [more]
Q101562.3e-9345.36Dual specificity protein kinase lkh1 OS=Schizosaccharomyces pombe (strain 972 / ... [more]
P497601.9e-9047.29Dual specificity protein kinase CLK2 OS=Homo sapiens OX=9606 GN=CLK2 PE=1 SV=1[more]
BLAST of MC08g0325 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022132125.12.21e-30299.75serine/threonine-protein kinase AFC3 [Momordica charantia][more]
XP_038886114.18.87e-29096.25serine/threonine-protein kinase AFC3 [Benincasa hispida][more]
XP_008445045.17.29e-28996.00PREDICTED: serine/threonine-protein kinase AFC3 [Cucumis melo][more]
XP_022996479.19.17e-28895.78serine/threonine-protein kinase AFC3 isoform X1 [Cucurbita maxima][more]
XP_004138782.14.92e-28795.50serine/threonine-protein kinase AFC3 [Cucumis sativus] >KGN62838.1 hypothetical ... [more]
BLAST of MC08g0325 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1BRK01.07e-30299.75serine/threonine-protein kinase AFC3 OS=Momordica charantia OX=3673 GN=LOC111005... [more]
A0A1S3BCM13.53e-28996.00serine/threonine-protein kinase AFC3 OS=Cucumis melo OX=3656 GN=LOC103488204 PE=... [more]
A0A6J1K8U54.44e-28895.78serine/threonine-protein kinase AFC3 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A0A0LM052.38e-28795.50Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G375... [more]
A0A5A7V9M08.08e-28794.61Serine/threonine-protein kinase AFC3 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
BLAST of MC08g0325 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G32660.15.6e-17572.57Protein kinase superfamily protein [more]
AT4G32660.32.8e-17473.54Protein kinase superfamily protein [more]
AT4G32660.21.4e-15773.18Protein kinase superfamily protein [more]
AT3G53570.31.4e-15464.79FUS3-complementing gene 1 [more]
AT3G53570.11.4e-15464.79FUS3-complementing gene 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 74..399
e-value: 2.5E-79
score: 279.5
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 74..399
e-value: 2.0E-51
score: 174.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 74..399
score: 39.537758
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 128..399
e-value: 2.9E-113
score: 380.5
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 61..154
e-value: 2.9E-113
score: 380.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..60
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 36..60
NoneNo IPR availablePANTHERPTHR45646SERINE/THREONINE-PROTEIN KINASE DOA-RELATEDcoord: 46..400
NoneNo IPR availablePANTHERPTHR45646:SF17BNAA07G37640D PROTEINcoord: 46..400
NoneNo IPR availableCDDcd14134PKc_CLKcoord: 61..399
e-value: 0.0
score: 537.919
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 195..207
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 61..402

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g0325.1MC08g0325.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity