MC08g0225 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g0225
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionPentatricopeptide repeat-containing protein
LocationMC08: 1639012 .. 1641478 (+)
RNA-Seq ExpressionMC08g0225
SyntenyMC08g0225
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTGGTGCTCCGGAGATCGCCGCTCGAACGGAGTCGATGATGAGGACGTATCTACGCTCCGGCGCCGGACGGTAAGCCTTTTACAGCTCTAAATTTTATCGTTTCATAGTCCGCTTCTGGTCTGAACCAGAGAGTTGTTTATTATTCTATAAATGCAGAGTTCACTTCCTTCTCGAAGCATGGAGGCCTATATCATCACTTATTTCGCCATCGCCAGTCAACTCTTTCTGCACCATTTCTGCGGTATCTCCCAAAACCCTAAAATACGGAGGCGACGATGACAACTTCAAGCGGGAATCTAAGAAAATCAAATTACCTCGGAGTAGTGTAACCACCGTGCTTCAGAGTGGCAACCGTGAGACTCAAAGGATTTCAATCACTAAGCTCCGGAAGGTCACCAAAAAGCTTTGCAAATCGAAGCGCTTTGCGTCAGCGCTAGAGGTTGGCAACTAGCAGTTTGGAAATTGAAATTTCTTACCTCGTTTTCATTTCAATGGAAGAATACATCAAGATCACAGAATATTTCATCTCACTATATATGATTCATAGATCGTGATAACAGGTAGAATTCAATATATTCTTATATCCAGGTACTAATGTTGATGGAAACTCGAAATAAATTGCGTATGTCACCGGCTGATCATGCTCTCAGATTGGAATTGACTATCAAACTGCACGGTTTACTGGAAGCTGAAGAGTACTTCAGTAAATTGCCCAACATAGCCTCTCAGAAAGCTTCATCTCTCCCTCTTCTTCATGGTTATGTCAAAGACAGGAACACCGAGAAAGCTGAGGCTTTCATGATGAAGCTTAAGGACTTGGGACTGGTTGTGGAGCCGCATCTTTACAATGAGATGATGAAACTGTATGTGGCGACATCTCAAAATGAGAAAGTTCCTCTTGTCATAAGGGACATGAAGCAAAATCAAATAATGAGAAATGTTCTCTCTTACAACCTTTGGATGAATGCTTGCAGTGAAATGTATGGGGTTGGGTCAGTGGAGTGGGTGCACAAAGAAATGCTGAAAGACGAAAACGTTCAAGTGGGATGGAGCACTCTATCTACTCTGGCTAAAGTTTACATAGAGGCAGGCGTTGTTGAGAAAGCGTTTTCAGCTTTAAAAGATGCAGAGAAGAAGCTATCCCCATGTAACAGGCTTGGATATTTCTTCTTGATCACATTATATGCTTCTCTGAACAATAAAGAGGGAGTTCTTCGACTTTGGAGAGCTAGCAAAGCTGTAATTGGCAGACCCACTTGTGCTAATTATATGTGTCTGCTTCTATGTTTGGTGAAGCTAGGAGAGATTGAAGAGGCTGAGAAAAAATTTAAGGAATGGGAACTCAGTTGTCGTAATTACGACATTAGAGTTTCCAATGTTCTTCTGGGTGCATATGTGAGGAATGGATTGTTAGAAAAAGCCGAGTTGTTGCATCTGCGCACATTGGAGAGAGGTGGGAGTCCAAATTATAAGACATGGGAGATTCTCATGGAGGGATGGATGAAGAGGCAAAACATGGATAGAGCTATTGATTCTCTCAAGAAAGATTATGATTTGTTGAAACACCACCCTGGTTAAAGTCGTCACTTCCTTTTGTTCTTGGAAGGCTTGTTGGTTTTGAGCTTATACATTGAAGGAGTGACTGAATTAATATGAAGGGCATTTCCTTTGCCATGGCCACAGTCTCATATGAGTTACAAACAGGGGATTTGGTAAGACAATTTGTGTAGAGGAAGCTGATGCAGTTGCAGTTGATGTGTTCTGTCCTGACGTCATGTGAAGTTGGATTTCATTTTCAGCAAGTGAAGGAACTGACACTGAAGGATCATGAACATTTTCTGATATACAATAACTCTGCTAAATGGGAGCCGAAGACACTGTTGATATGATCCATTGCATATGGTTCGATAGAACATCTATCTATATATGCCGTTAATCTGCTTGTAATGGTGCAATCACTGTGAAGCATAGATTTGTCGCAATGGGAATAAGATGGATTCATAGGTGGTATGCTATTATTGTCTGTCCCTTTCCTTATTCATTCAGGGCTTCTAAGTGTCCTTTGTACTGTTTGCAAATCATCGAGTCGTGTTTAAGTAAATTGGGTTAGGTTTGGTTGCTAATGGAATAGAATCCGTTGACAGCAGCATAAATGACTAGAATTGGAAGTGGTGCATATCGTGGTTTTTGCCAGCTGAGAATAAAGTTACTGTGTATTTTCCAGCAACGAAAGAAGCATCCTGGTCCAATTTCAAGCATTTTCAGAATATTTCGTTATTTGGAGTAGTTTTTCATTTTCACTTATTGTTACGAATATAGCCTTTACTTTCCTCTCGAACAGATTTTCATACCATGATTAGTATAGTCCCTGAGTTAAGTTGATAGGTTGACTAATTGTAACATCAACCACCACTCCTTGCCAACAATTTTTCTCTCTTTTCCTATCTTTTCTACTTTTTTCTCCG

mRNA sequence

CTTGGTGCTCCGGAGATCGCCGCTCGAACGGAGTCGATGATGAGGACGTATCTACGCTCCGGCGCCGGACGAGTTCACTTCCTTCTCGAAGCATGGAGGCCTATATCATCACTTATTTCGCCATCGCCAGTCAACTCTTTCTGCACCATTTCTGCGGTATCTCCCAAAACCCTAAAATACGGAGGCGACGATGACAACTTCAAGCGGGAATCTAAGAAAATCAAATTACCTCGGAGTAGTGTAACCACCGTGCTTCAGAGTGGCAACCGTGAGACTCAAAGGATTTCAATCACTAAGCTCCGGAAGGTCACCAAAAAGCTTTGCAAATCGAAGCGCTTTGCGTCAGCGCTAGAGGTACTAATGTTGATGGAAACTCGAAATAAATTGCGTATGTCACCGGCTGATCATGCTCTCAGATTGGAATTGACTATCAAACTGCACGGTTTACTGGAAGCTGAAGAGTACTTCAGTAAATTGCCCAACATAGCCTCTCAGAAAGCTTCATCTCTCCCTCTTCTTCATGGTTATGTCAAAGACAGGAACACCGAGAAAGCTGAGGCTTTCATGATGAAGCTTAAGGACTTGGGACTGGTTGTGGAGCCGCATCTTTACAATGAGATGATGAAACTGTATGTGGCGACATCTCAAAATGAGAAAGTTCCTCTTGTCATAAGGGACATGAAGCAAAATCAAATAATGAGAAATGTTCTCTCTTACAACCTTTGGATGAATGCTTGCAGTGAAATGTATGGGGTTGGGTCAGTGGAGTGGGTGCACAAAGAAATGCTGAAAGACGAAAACGTTCAAGTGGGATGGAGCACTCTATCTACTCTGGCTAAAGTTTACATAGAGGCAGGCGTTGTTGAGAAAGCGTTTTCAGCTTTAAAAGATGCAGAGAAGAAGCTATCCCCATGTAACAGGCTTGGATATTTCTTCTTGATCACATTATATGCTTCTCTGAACAATAAAGAGGGAGTTCTTCGACTTTGGAGAGCTAGCAAAGCTGTAATTGGCAGACCCACTTGTGCTAATTATATGTGTCTGCTTCTATGTTTGGTGAAGCTAGGAGAGATTGAAGAGGCTGAGAAAAAATTTAAGGAATGGGAACTCAGTTGTCGTAATTACGACATTAGAGTTTCCAATGTTCTTCTGGGTGCATATGTGAGGAATGGATTGTTAGAAAAAGCCGAGTTGTTGCATCTGCGCACATTGGAGAGAGGTGGGAGTCCAAATTATAAGACATGGGAGATTCTCATGGAGGGATGGATGAAGAGGCAAAACATGGATAGAGCTATTGATTCTCTCAAGAAAGATTATGATTTGTTGAAACACCACCCTGGTTAAAGTCGTCACTTCCTTTTGTTCTTGGAAGGCTTGTTGGTTTTGAGCTTATACATTGAAGGAGTGACTGAATTAATATGAAGGGCATTTCCTTTGCCATGGCCACAGTCTCATATGAGTTACAAACAGGGGATTTGGTAAGACAATTTGTGTAGAGGAAGCTGATGCAGTTGCAGTTGATGTGTTCTGTCCTGACGTCATGTGAAGTTGGATTTCATTTTCAGCAAGTGAAGGAACTGACACTGAAGGATCATGAACATTTTCTGATATACAATAACTCTGCTAAATGGGAGCCGAAGACACTGTTGATATGATCCATTGCATATGGTTCGATAGAACATCTATCTATATATGCCGTTAATCTGCTTGTAATGGTGCAATCACTGTGAAGCATAGATTTGTCGCAATGGGAATAAGATGGATTCATAGGTGGTATGCTATTATTGTCTGTCCCTTTCCTTATTCATTCAGGGCTTCTAAGTGTCCTTTGTACTGTTTGCAAATCATCGAGTCGTGTTTAAGTAAATTGGGTTAGGTTTGGTTGCTAATGGAATAGAATCCGTTGACAGCAGCATAAATGACTAGAATTGGAAGTGGTGCATATCGTGGTTTTTGCCAGCTGAGAATAAAGTTACTGTGTATTTTCCAGCAACGAAAGAAGCATCCTGGTCCAATTTCAAGCATTTTCAGAATATTTCGTTATTTGGAGTAGTTTTTCATTTTCACTTATTGTTACGAATATAGCCTTTACTTTCCTCTCGAACAGATTTTCATACCATGATTAGTATAGTCCCTGAGTTAAGTTGATAGGTTGACTAATTGTAACATCAACCACCACTCCTTGCCAACAATTTTTCTCTCTTTTCCTATCTTTTCTACTTTTTTCTCCG

Coding sequence (CDS)

CTTGGTGCTCCGGAGATCGCCGCTCGAACGGAGTCGATGATGAGGACGTATCTACGCTCCGGCGCCGGACGAGTTCACTTCCTTCTCGAAGCATGGAGGCCTATATCATCACTTATTTCGCCATCGCCAGTCAACTCTTTCTGCACCATTTCTGCGGTATCTCCCAAAACCCTAAAATACGGAGGCGACGATGACAACTTCAAGCGGGAATCTAAGAAAATCAAATTACCTCGGAGTAGTGTAACCACCGTGCTTCAGAGTGGCAACCGTGAGACTCAAAGGATTTCAATCACTAAGCTCCGGAAGGTCACCAAAAAGCTTTGCAAATCGAAGCGCTTTGCGTCAGCGCTAGAGGTACTAATGTTGATGGAAACTCGAAATAAATTGCGTATGTCACCGGCTGATCATGCTCTCAGATTGGAATTGACTATCAAACTGCACGGTTTACTGGAAGCTGAAGAGTACTTCAGTAAATTGCCCAACATAGCCTCTCAGAAAGCTTCATCTCTCCCTCTTCTTCATGGTTATGTCAAAGACAGGAACACCGAGAAAGCTGAGGCTTTCATGATGAAGCTTAAGGACTTGGGACTGGTTGTGGAGCCGCATCTTTACAATGAGATGATGAAACTGTATGTGGCGACATCTCAAAATGAGAAAGTTCCTCTTGTCATAAGGGACATGAAGCAAAATCAAATAATGAGAAATGTTCTCTCTTACAACCTTTGGATGAATGCTTGCAGTGAAATGTATGGGGTTGGGTCAGTGGAGTGGGTGCACAAAGAAATGCTGAAAGACGAAAACGTTCAAGTGGGATGGAGCACTCTATCTACTCTGGCTAAAGTTTACATAGAGGCAGGCGTTGTTGAGAAAGCGTTTTCAGCTTTAAAAGATGCAGAGAAGAAGCTATCCCCATGTAACAGGCTTGGATATTTCTTCTTGATCACATTATATGCTTCTCTGAACAATAAAGAGGGAGTTCTTCGACTTTGGAGAGCTAGCAAAGCTGTAATTGGCAGACCCACTTGTGCTAATTATATGTGTCTGCTTCTATGTTTGGTGAAGCTAGGAGAGATTGAAGAGGCTGAGAAAAAATTTAAGGAATGGGAACTCAGTTGTCGTAATTACGACATTAGAGTTTCCAATGTTCTTCTGGGTGCATATGTGAGGAATGGATTGTTAGAAAAAGCCGAGTTGTTGCATCTGCGCACATTGGAGAGAGGTGGGAGTCCAAATTATAAGACATGGGAGATTCTCATGGAGGGATGGATGAAGAGGCAAAACATGGATAGAGCTATTGATTCTCTCAAGAAAGATTATGATTTGTTGAAACACCACCCTGGTTAA

Protein sequence

LGAPEIAARTESMMRTYLRSGAGRVHFLLEAWRPISSLISPSPVNSFCTISAVSPKTLKYGGDDDNFKRESKKIKLPRSSVTTVLQSGNRETQRISITKLRKVTKKLCKSKRFASALEVLMLMETRNKLRMSPADHALRLELTIKLHGLLEAEEYFSKLPNIASQKASSLPLLHGYVKDRNTEKAEAFMMKLKDLGLVVEPHLYNEMMKLYVATSQNEKVPLVIRDMKQNQIMRNVLSYNLWMNACSEMYGVGSVEWVHKEMLKDENVQVGWSTLSTLAKVYIEAGVVEKAFSALKDAEKKLSPCNRLGYFFLITLYASLNNKEGVLRLWRASKAVIGRPTCANYMCLLLCLVKLGEIEEAEKKFKEWELSCRNYDIRVSNVLLGAYVRNGLLEKAELLHLRTLERGGSPNYKTWEILMEGWMKRQNMDRAIDSLKKDYDLLKHHPG
Homology
BLAST of MC08g0225 vs. ExPASy Swiss-Prot
Match: Q3E911 (Pentatricopeptide repeat-containing protein At5g27460 OS=Arabidopsis thaliana OX=3702 GN=At5g27460 PE=2 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 3.9e-113
Identity = 210/371 (56.60%), Postives = 275/371 (74.12%), Query Frame = 0

Query: 77  PRSSVTTVLQSGNRETQRISITKLRKVTKKLCKSKRFASALEVLMLMETRNKLRMSPADH 136
           PR SVT++LQ        +S+++LR ++K+L +S R+  AL+++  ME +  +  S  D 
Sbjct: 50  PRRSVTSLLQERIDSGHAVSLSELRLISKRLIRSNRYDLALQMMEWMENQKDIEFSVYDI 109

Query: 137 ALRLELTIKLHGLLEAEEYFSKL----PNIASQKASSLPLLHGYVKDRNTEKAEAFMMKL 196
           ALRL+L IK HGL + EEYF KL     ++   K++ LPLL  YVK++  ++AEA M KL
Sbjct: 110 ALRLDLIIKTHGLKQGEEYFEKLLHSSVSMRVAKSAYLPLLRAYVKNKMVKEAEALMEKL 169

Query: 197 KDLGLVVEPHLYNEMMKLYVATSQNEKVPLVIRDMKQNQIMRNVLSYNLWMNACSEMYGV 256
             LG +V PH +NEMMKLY A+ Q EKV +V+  MK N+I RNVLSYNLWMNAC E+ GV
Sbjct: 170 NGLGFLVTPHPFNEMMKLYEASGQYEKVVMVVSMMKGNKIPRNVLSYNLWMNACCEVSGV 229

Query: 257 GSVEWVHKEMLKDENVQVGWSTLSTLAKVYIEAGVVEKAFSALKDAEKKLSPCNRLGYFF 316
            +VE V+KEM+ D++V+VGWS+L TLA VYI++G  EKA   L+DAEK L+  NRLGYFF
Sbjct: 230 AAVETVYKEMVGDKSVEVGWSSLCTLANVYIKSGFDEKARLVLEDAEKMLNRSNRLGYFF 289

Query: 317 LITLYASLNNKEGVLRLWRASKAVIGRPTCANYMCLLLCLVKLGEIEEAEKKFKEWELSC 376
           LITLYASL NKEGV+RLW  SK+V GR +C NY+C+L  LVK G++EEAE+ F EWE  C
Sbjct: 290 LITLYASLGNKEGVVRLWEVSKSVCGRISCVNYICVLSSLVKTGDLEEAERVFSEWEAQC 349

Query: 377 RNYDIRVSNVLLGAYVRNGLLEKAELLHLRTLERGGSPNYKTWEILMEGWMKRQNMDRAI 436
            NYD+RVSNVLLGAYVRNG + KAE LH   LERGG+PNYKTWEILMEGW+K +NM++AI
Sbjct: 350 FNYDVRVSNVLLGAYVRNGEIRKAESLHGCVLERGGTPNYKTWEILMEGWVKCENMEKAI 409

Query: 437 DSLKKDYDLLK 444
           D++ + + L++
Sbjct: 410 DAMHQVFVLMR 420

BLAST of MC08g0225 vs. ExPASy Swiss-Prot
Match: Q8LPS6 (Pentatricopeptide repeat-containing protein At1g02150 OS=Arabidopsis thaliana OX=3702 GN=At1g02150 PE=2 SV=2)

HSP 1 Score: 239.6 bits (610), Expect = 6.9e-62
Identity = 145/426 (34.04%), Postives = 238/426 (55.87%), Query Frame = 0

Query: 28  LLEAWRPISSL--ISPSPVNSFCTISAVSPKTLKYGGDDDNFKRES--------KKIKL- 87
           LL    P+ S+  +S       C+IS V      YG    +++R          KKI L 
Sbjct: 23  LLRCRSPVVSVAALSKKTAAIVCSISQV------YGYGTVDYERRPIVQWNAIYKKISLM 82

Query: 88  --PRSSVTTVLQSGNRETQRISITKLRKVTKKLCKSKRFASALEVLMLMETR-NKLRMSP 147
             P     +VL    +  ++++  +L +V K+L K KR   ALEV   M  R  + R+S 
Sbjct: 83  EKPELGAASVLNQWEKAGRKLTKWELCRVVKELRKYKRANQALEVYDWMNNRGERFRLSA 142

Query: 148 ADHALRLELTIKLHGLLEAEEYFSKLPNIASQKASSLPLLHGYVKDRNTEKAEAFMMKLK 207
           +D A++L+L  K+ G+ +AEE+F +LP     +     LL+ YV+ ++ EKAEA +  ++
Sbjct: 143 SDAAIQLDLIGKVRGIPDAEEFFLQLPENFKDRRVYGSLLNAYVRAKSREKAEALLNTMR 202

Query: 208 DLGLVVEPHLYNEMMKLYVATSQNEKVPLVIRDMKQNQIMRNVLSYNLWMNACSEMYGVG 267
           D G  + P  +N MM LY+   + +KV  ++ +MKQ  I  ++ SYN+W+++C  +  V 
Sbjct: 203 DKGYALHPLPFNVMMTLYMNLREYDKVDAMVFEMKQKDIRLDIYSYNIWLSSCGSLGSVE 262

Query: 268 SVEWVHKEMLKDENVQVGWSTLSTLAKVYIEAGVVEKAFSALKDAEKKLSPCNRLGYFFL 327
            +E V+++M  D ++   W+T ST+A +YI+ G  EKA  AL+  E +++  NR+ Y +L
Sbjct: 263 KMELVYQQMKSDVSIYPNWTTFSTMATMYIKMGETEKAEDALRKVEARITGRNRIPYHYL 322

Query: 328 ITLYASLNNKEGVLRLWRASKAVIGRPTCANYMCLLLCLVKLGEIEEAEKKFKEWELSCR 387
           ++LY SL NK+ + R+W   K+V+       Y  L+  LV++G+IE AEK ++EW     
Sbjct: 323 LSLYGSLGNKKELYRVWHVYKSVVPSIPNLGYHALVSSLVRMGDIEGAEKVYEEWLPVKS 382

Query: 388 NYDIRVSNVLLGAYVRNGLLEKAELLHLRTLERGGSPNYKTWEILMEGWMKRQNMDRAID 440
           +YD R+ N+L+ AYV+N  LE AE L    +E GG P+  TWEIL  G  +++ +  A+ 
Sbjct: 383 SYDPRIPNLLMNAYVKNDQLETAEGLFDHMVEMGGKPSSSTWEILAVGHTRKRCISEALT 442

BLAST of MC08g0225 vs. ExPASy Swiss-Prot
Match: O22714 (Pentatricopeptide repeat-containing protein At1g60770 OS=Arabidopsis thaliana OX=3702 GN=At1g60770 PE=1 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 7.1e-59
Identity = 125/343 (36.44%), Postives = 195/343 (56.85%), Query Frame = 0

Query: 105 KKLCKSKRFASALEVLMLMETRNKLRMSPADHALRLELTIKLHGLLEAEEYFSKLPNIAS 164
           KKL     +  AL++  +ME R  +  + +D A+ L+L  K   +   E YF  LP  + 
Sbjct: 63  KKLRNRGLYYPALKLSEVMEERG-MNKTVSDQAIHLDLVAKAREITAGENYFVDLPETSK 122

Query: 165 QKASSLPLLHGYVKDRNTEKAEAFMMKLKDLGLVVEPHLYNEMMKLYVATSQNEKVPLVI 224
            + +   LL+ Y K+  TEKAE  + K+K+L +      YN +M LY  T + EKVP +I
Sbjct: 123 TELTYGSLLNCYCKELLTEKAEGLLNKMKELNITPSSMSYNSLMTLYTKTGETEKVPAMI 182

Query: 225 RDMKQNQIMRNVLSYNLWMNACSEMYGVGSVEWVHKEMLKDENVQVGWSTLSTLAKVYIE 284
           +++K   +M +  +YN+WM A +    +  VE V +EM +D  V   W+T S +A +Y++
Sbjct: 183 QELKAENVMPDSYTYNVWMRALAATNDISGVERVIEEMNRDGRVAPDWTTYSNMASIYVD 242

Query: 285 AGVVEKAFSALKDAEKKLSPCNRLGYFFLITLYASLNNKEGVLRLWRASKAVIGRPTCAN 344
           AG+ +KA  AL++ E K +  +   Y FLITLY  L     V R+WR+ +  I + +   
Sbjct: 243 AGLSQKAEKALQELEMKNTQRDFTAYQFLITLYGRLGKLTEVYRIWRSLRLAIPKTSNVA 302

Query: 345 YMCLLLCLVKLGEIEEAEKKFKEWELSCRNYDIRVSNVLLGAYVRNGLLEKAELLHLRTL 404
           Y+ ++  LVKL ++  AE  FKEW+ +C  YDIR+ NVL+GAY + GL++KA  L  +  
Sbjct: 303 YLNMIQVLVKLNDLPGAETLFKEWQANCSTYDIRIVNVLIGAYAQEGLIQKANELKEKAP 362

Query: 405 ERGGSPNYKTWEILMEGWMKRQNMDRAIDSLKKDYDLLKHHPG 448
            RGG  N KTWEI M+ ++K  +M RA++ + K   + K   G
Sbjct: 363 RRGGKLNAKTWEIFMDYYVKSGDMARALECMSKAVSIGKGDGG 404

BLAST of MC08g0225 vs. ExPASy Swiss-Prot
Match: Q9SY07 (Pentatricopeptide repeat-containing protein At4g02820, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g02820 PE=2 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 2.8e-55
Identity = 126/392 (32.14%), Postives = 219/392 (55.87%), Query Frame = 0

Query: 52  AVSPKTLKYGGDDDNFKRESKKIKLPRSSVTTVLQSGNRETQRISITKLRKVTKKLCKSK 111
           + + K    GG D    R    +   RS+V T+ +    E   +   +L ++ ++L K K
Sbjct: 49  SANKKETVVGGRDTLGGRLLSLVYTKRSAVVTI-RKWKEEGHSVRKYELNRIVRELRKIK 108

Query: 112 RFASALEVLMLMETRNKLRMSPADHALRLELTIKLHGLLEAEEYFSKLPNIASQKASSLP 171
           R+  ALE+   M  +  +++   D+A+ L+L  K+ GL  AE++F  +P+     A+   
Sbjct: 109 RYKHALEICEWMVVQEDIKLQAGDYAVHLDLISKIRGLNSAEKFFEDMPDQMRGHAACTS 168

Query: 172 LLHGYVKDRNTEKAEAFMMKLKDLGLVVEPHLYNEMMKLYVATSQNEKVPLVIRDMKQNQ 231
           LLH YV+++ ++KAEA   K+ + G +     YN M+ +Y++  Q EKVP++I+++K  +
Sbjct: 169 LLHSYVQNKLSDKAEALFEKMGECGFLKSCLPYNHMLSMYISRGQFEKVPVLIKELK-IR 228

Query: 232 IMRNVLSYNLWMNACSEMYGVGSVEWVHKEMLKDENVQVGWSTLSTLAKVYIEAGVVEKA 291
              ++++YNLW+ A +    V   E V+ +  K+E +   W T S L  +Y +   VEKA
Sbjct: 229 TSPDIVTYNLWLTAFASGNDVEGAEKVYLK-AKEEKLNPDWVTYSVLTNLYAKTDNVEKA 288

Query: 292 FSALKDAEKKLSPCNRLGYFFLITLYASLNNKEGVLRLWRASKAVIGRPTCANYMCLLLC 351
             ALK+ EK +S  NR+ Y  LI+L+A+L +K+GV   W+  K+   +   A Y+ ++  
Sbjct: 289 RLALKEMEKLVSKKNRVAYASLISLHANLGDKDGVNLTWKKVKSSFKKMNDAEYLSMISA 348

Query: 352 LVKLGEIEEAEKKFKEWELSCRNYDIRVSNVLLGAYVRNGLLEKAELLHLRTLERGGSPN 411
           +VKLGE E+A+  + EWE      D R+ N++L  Y+    +   E  + R +E+G +P+
Sbjct: 349 VVKLGEFEQAKGLYDEWESVSGTGDARIPNLILAEYMNRDEVLLGEKFYERIVEKGINPS 408

Query: 412 YKTWEILMEGWMKRQNMDRAIDSLKKDYDLLK 444
           Y TWEIL   ++KR++M++ +D   K  D +K
Sbjct: 409 YSTWEILTWAYLKRKDMEKVLDCFGKAIDSVK 437

BLAST of MC08g0225 vs. ExPASy Swiss-Prot
Match: Q84JR3 (Pentatricopeptide repeat-containing protein At4g21705, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g21705 PE=2 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 3.2e-51
Identity = 118/360 (32.78%), Postives = 193/360 (53.61%), Query Frame = 0

Query: 77  PRSSVTTVLQSGNRETQRISITKLRKVTKKLCKSKRFASALEVLMLMETRNKLRMSPADH 136
           P+SSV   LQ+  +  +++S+ +L ++   L + KRF  ALEV   M        SP +H
Sbjct: 37  PKSSVYPELQNWVQCGKKVSVAELIRIVHDLRRRKRFLHALEVSKWMNETGVCVFSPTEH 96

Query: 137 ALRLELTIKLHGLLEAEEYFSKLPNIASQKASSLPLLHGYVKDRNTEKAEAFMMKLKDLG 196
           A+ L+L  +++G + AEEYF  L        +   LL+ YV+ +N EK+     K+K++G
Sbjct: 97  AVHLDLIGRVYGFVTAEEYFENLKEQYKNDKTYGALLNCYVRQQNVEKSLLHFEKMKEMG 156

Query: 197 LVVEPHLYNEMMKLYVATSQNEKVPLVIRDMKQNQIMRNVLSYNLWMNACSEMYGVGSVE 256
            V     YN +M LY    Q+EKVP V+ +MK+  +  +  SY + +NA   MY +  + 
Sbjct: 157 FVTSSLTYNNIMCLYTNIGQHEKVPKVLEEMKEENVAPDNYSYRICINAFGAMYDLERIG 216

Query: 257 WVHKEMLKDENVQVGWSTLSTLAKVYIEAGVVEKAFSALKDAEKKLSPCNRLGYFFLITL 316
              ++M + +++ + W+T +  AK YI+ G  ++A   LK +E +L   +  GY  LITL
Sbjct: 217 GTLRDMERRQDITMDWNTYAVAAKFYIDGGDCDRAVELLKMSENRLEKKDGEGYNHLITL 276

Query: 317 YASLNNKEGVLRLWRASKAVIGRPTCANYMCLLLCLVKLGEIEEAEKKFKEWELSCRNYD 376
           YA L  K  VLRLW   K V  R    +Y+ +L  LVK+  + EAE+   EW+ S   YD
Sbjct: 277 YARLGKKIEVLRLWDLEKDVCKRRINQDYLTVLQSLVKIDALVEAEEVLTEWKSSGNCYD 336

Query: 377 IRVSNVLLGAYVRNGLLEKAELLHLRTLERGGSPNYKTWEILMEGWMKRQNMDRAIDSLK 436
            RV N ++  Y+   + EKAE +      RG +   ++WE++   + ++  ++ A   +K
Sbjct: 337 FRVPNTVIRGYIGKSMEEKAEAMLEDLARRGKATTPESWELVATAYAEKGTLENAFKCMK 396

BLAST of MC08g0225 vs. NCBI nr
Match: XP_022132148.1 (pentatricopeptide repeat-containing protein At5g27460-like [Momordica charantia])

HSP 1 Score: 867 bits (2241), Expect = 0.0
Identity = 435/435 (100.00%), Postives = 435/435 (100.00%), Query Frame = 0

Query: 13  MMRTYLRSGAGRVHFLLEAWRPISSLISPSPVNSFCTISAVSPKTLKYGGDDDNFKRESK 72
           MMRTYLRSGAGRVHFLLEAWRPISSLISPSPVNSFCTISAVSPKTLKYGGDDDNFKRESK
Sbjct: 1   MMRTYLRSGAGRVHFLLEAWRPISSLISPSPVNSFCTISAVSPKTLKYGGDDDNFKRESK 60

Query: 73  KIKLPRSSVTTVLQSGNRETQRISITKLRKVTKKLCKSKRFASALEVLMLMETRNKLRMS 132
           KIKLPRSSVTTVLQSGNRETQRISITKLRKVTKKLCKSKRFASALEVLMLMETRNKLRMS
Sbjct: 61  KIKLPRSSVTTVLQSGNRETQRISITKLRKVTKKLCKSKRFASALEVLMLMETRNKLRMS 120

Query: 133 PADHALRLELTIKLHGLLEAEEYFSKLPNIASQKASSLPLLHGYVKDRNTEKAEAFMMKL 192
           PADHALRLELTIKLHGLLEAEEYFSKLPNIASQKASSLPLLHGYVKDRNTEKAEAFMMKL
Sbjct: 121 PADHALRLELTIKLHGLLEAEEYFSKLPNIASQKASSLPLLHGYVKDRNTEKAEAFMMKL 180

Query: 193 KDLGLVVEPHLYNEMMKLYVATSQNEKVPLVIRDMKQNQIMRNVLSYNLWMNACSEMYGV 252
           KDLGLVVEPHLYNEMMKLYVATSQNEKVPLVIRDMKQNQIMRNVLSYNLWMNACSEMYGV
Sbjct: 181 KDLGLVVEPHLYNEMMKLYVATSQNEKVPLVIRDMKQNQIMRNVLSYNLWMNACSEMYGV 240

Query: 253 GSVEWVHKEMLKDENVQVGWSTLSTLAKVYIEAGVVEKAFSALKDAEKKLSPCNRLGYFF 312
           GSVEWVHKEMLKDENVQVGWSTLSTLAKVYIEAGVVEKAFSALKDAEKKLSPCNRLGYFF
Sbjct: 241 GSVEWVHKEMLKDENVQVGWSTLSTLAKVYIEAGVVEKAFSALKDAEKKLSPCNRLGYFF 300

Query: 313 LITLYASLNNKEGVLRLWRASKAVIGRPTCANYMCLLLCLVKLGEIEEAEKKFKEWELSC 372
           LITLYASLNNKEGVLRLWRASKAVIGRPTCANYMCLLLCLVKLGEIEEAEKKFKEWELSC
Sbjct: 301 LITLYASLNNKEGVLRLWRASKAVIGRPTCANYMCLLLCLVKLGEIEEAEKKFKEWELSC 360

Query: 373 RNYDIRVSNVLLGAYVRNGLLEKAELLHLRTLERGGSPNYKTWEILMEGWMKRQNMDRAI 432
           RNYDIRVSNVLLGAYVRNGLLEKAELLHLRTLERGGSPNYKTWEILMEGWMKRQNMDRAI
Sbjct: 361 RNYDIRVSNVLLGAYVRNGLLEKAELLHLRTLERGGSPNYKTWEILMEGWMKRQNMDRAI 420

Query: 433 DSLKKDYDLLKHHPG 447
           DSLKKDYDLLKHHPG
Sbjct: 421 DSLKKDYDLLKHHPG 435

BLAST of MC08g0225 vs. NCBI nr
Match: XP_022997144.1 (pentatricopeptide repeat-containing protein At5g27460 isoform X1 [Cucurbita maxima])

HSP 1 Score: 693 bits (1788), Expect = 3.57e-248
Identity = 350/445 (78.65%), Postives = 397/445 (89.21%), Query Frame = 0

Query: 1   LGAPEIAARTESMMRTYLRSGAGRVHFLLEAWRPISSLISPSPVNSFCTISAVSPKTLKY 60
           LGA E+AART+S+M+T LRSG GR+HFLL AWRPISS    SPV+S   ISAVSPKTL+ 
Sbjct: 5   LGASEVAARTKSVMKTTLRSGTGRIHFLLRAWRPISS--HTSPVDSSDIISAVSPKTLQK 64

Query: 61  GGDDDNFKRESKKIKLPRSSVTTVLQSGNRETQRISITKLRKVTKKLCKSKRFASALEVL 120
           GGD+ NFK++  K+K PRSSVTTVLQSGN E QR SITKLR+V KKLCKSKRF  ALEVL
Sbjct: 65  GGDNGNFKQDFMKLKQPRSSVTTVLQSGNSEAQRTSITKLRRVVKKLCKSKRFERALEVL 124

Query: 121 MLMETRNKLRMSPADHALRLELTIKLHGLLEAEEYFSKLPNIASQKASSLPLLHGYVKDR 180
           MLMET+NK+RMSPADHALRLELTIK+HGLL+AEE+F++LPNIASQKASSL LLHGYVK++
Sbjct: 125 MLMETQNKIRMSPADHALRLELTIKVHGLLKAEEHFNQLPNIASQKASSLLLLHGYVKEQ 184

Query: 181 NTEKAEAFMMKLKDLGLVVEPHLYNEMMKLYVATSQNEKVPLVIRDMKQNQIMRNVLSYN 240
           NTEKAEAFM KLK LGLVV+PHLYNEMMKLYVATSQNEKVPLVI+DMKQNQI+RNVLSYN
Sbjct: 185 NTEKAEAFMAKLKGLGLVVDPHLYNEMMKLYVATSQNEKVPLVIKDMKQNQILRNVLSYN 244

Query: 241 LWMNACSEMYGVGSVEWVHKEMLKDENVQVGWSTLSTLAKVYIEAGVVEKAFSALKDAEK 300
           LWMNACSE+YGVGSVE V++EML D+NVQVGWSTLSTLAKVY +AG+V KAF+AL++AEK
Sbjct: 245 LWMNACSELYGVGSVELVYEEMLSDKNVQVGWSTLSTLAKVYTQAGLVHKAFAALREAEK 304

Query: 301 KLSPCNRLGYFFLITLYASLNNKEGVLRLWRASKAVIGRPTCANYMCLLLCLVKLGEIEE 360
           KLS  NRLGYFFLITLYA+LN+KEGVLR+W ASKAV  RPTCANYMC+LLCLVKLG+I E
Sbjct: 305 KLSSGNRLGYFFLITLYATLNDKEGVLRVWTASKAVSDRPTCANYMCILLCLVKLGDINE 364

Query: 361 AEKKFKEWELSCRNYDIRVSNVLLGAYVRNGLLEKAELLHLRTLERGGSPNYKTWEILME 420
           AEK FKEWEL CRNYDIRVSNVLLGAYVRNGLL++AE LH+ TLERGG+PNYKTWEILME
Sbjct: 365 AEKIFKEWELDCRNYDIRVSNVLLGAYVRNGLLDQAESLHMHTLERGGTPNYKTWEILME 424

Query: 421 GWMKRQNMDRAIDSLKKDYDLLKHH 445
           G ++ QN+DR I+ L  D+  LK H
Sbjct: 425 GCVRSQNLDRGINFLNNDFAGLKCH 447

BLAST of MC08g0225 vs. NCBI nr
Match: XP_023545813.1 (pentatricopeptide repeat-containing protein At5g27460 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 690 bits (1780), Expect = 5.89e-247
Identity = 352/445 (79.10%), Postives = 393/445 (88.31%), Query Frame = 0

Query: 1   LGAPEIAARTESMMRTYLRSGAGRVHFLLEAWRPISSLISPSPVNSFCTISAVSPKTLKY 60
           LGA E+AART S+M+T LRSG GR+HFLL AWRPISS    SPV+S   ISAVSPKTL+ 
Sbjct: 5   LGASEVAARTNSVMKTTLRSGTGRIHFLLRAWRPISS--HTSPVDSSDIISAVSPKTLQK 64

Query: 61  GGDDDNFKRESKKIKLPRSSVTTVLQSGNRETQRISITKLRKVTKKLCKSKRFASALEVL 120
           GGD+ NFK++  K+K PRSSVTTVLQSGN E QR SITKLR+V KKLCKSKRF  ALEVL
Sbjct: 65  GGDNGNFKQDFMKLKSPRSSVTTVLQSGNCEAQRTSITKLRRVVKKLCKSKRFERALEVL 124

Query: 121 MLMETRNKLRMSPADHALRLELTIKLHGLLEAEEYFSKLPNIASQKASSLPLLHGYVKDR 180
           MLMET+NK+RMSPADHALRLELTIKLHGLL+AEE F++LPNIASQKASSL LLHGYVK++
Sbjct: 125 MLMETQNKIRMSPADHALRLELTIKLHGLLKAEELFNQLPNIASQKASSLLLLHGYVKEK 184

Query: 181 NTEKAEAFMMKLKDLGLVVEPHLYNEMMKLYVATSQNEKVPLVIRDMKQNQIMRNVLSYN 240
           NTEKAEAFM KLK LGLVV+PHLYNEMMKLYVATSQNEKVPLVI+DMKQN I+RNVLSYN
Sbjct: 185 NTEKAEAFMAKLKGLGLVVDPHLYNEMMKLYVATSQNEKVPLVIKDMKQNHILRNVLSYN 244

Query: 241 LWMNACSEMYGVGSVEWVHKEMLKDENVQVGWSTLSTLAKVYIEAGVVEKAFSALKDAEK 300
           LWMNACSE+YGV SVE V++EML D+NVQVGWSTLSTLAKVY +AG+V KAF+ALK AEK
Sbjct: 245 LWMNACSELYGVRSVELVYEEMLSDKNVQVGWSTLSTLAKVYTQAGLVHKAFAALKAAEK 304

Query: 301 KLSPCNRLGYFFLITLYASLNNKEGVLRLWRASKAVIGRPTCANYMCLLLCLVKLGEIEE 360
           KLS  NRLGYFFLITLYA+LN+KEGVLR+W ASKAV  RPTCANYMC+LLCLVKLG+I E
Sbjct: 305 KLSSGNRLGYFFLITLYATLNDKEGVLRVWTASKAVSDRPTCANYMCILLCLVKLGDIHE 364

Query: 361 AEKKFKEWELSCRNYDIRVSNVLLGAYVRNGLLEKAELLHLRTLERGGSPNYKTWEILME 420
           AEK F+EWEL CRNYDIRVSNVLLGAYVRNGLLEKAE LH+ TLERGG+PNYKTWEILME
Sbjct: 365 AEKIFQEWELDCRNYDIRVSNVLLGAYVRNGLLEKAESLHMHTLERGGTPNYKTWEILME 424

Query: 421 GWMKRQNMDRAIDSLKKDYDLLKHH 445
           G ++ QN+DR I+ LK D+  LK H
Sbjct: 425 GCVRSQNLDRGINFLKNDFAGLKCH 447

BLAST of MC08g0225 vs. NCBI nr
Match: KAG6598713.1 (Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 689 bits (1778), Expect = 1.19e-246
Identity = 350/445 (78.65%), Postives = 395/445 (88.76%), Query Frame = 0

Query: 1   LGAPEIAARTESMMRTYLRSGAGRVHFLLEAWRPISSLISPSPVNSFCTISAVSPKTLKY 60
           LGA E+AART+S+M+T LRSGAGR+HFLL AWRPISS    SPV+S   ISAVSPKTL+ 
Sbjct: 5   LGASEVAARTKSVMKTTLRSGAGRIHFLLRAWRPISS--HTSPVDSSDIISAVSPKTLQK 64

Query: 61  GGDDDNFKRESKKIKLPRSSVTTVLQSGNRETQRISITKLRKVTKKLCKSKRFASALEVL 120
           GGD+ NFK++  K+K PRSSVTTVLQSGN E QR SITKLR+V KKLCKSKRF  ALE L
Sbjct: 65  GGDNGNFKQDFMKLKQPRSSVTTVLQSGNSEAQRTSITKLRRVVKKLCKSKRFERALEAL 124

Query: 121 MLMETRNKLRMSPADHALRLELTIKLHGLLEAEEYFSKLPNIASQKASSLPLLHGYVKDR 180
           MLMET+NK+RMSPADHALRLELTIK+HGLL+AEE+F++LPNIASQKASSL LLHGYVK++
Sbjct: 125 MLMETQNKIRMSPADHALRLELTIKVHGLLKAEEHFNQLPNIASQKASSLLLLHGYVKEK 184

Query: 181 NTEKAEAFMMKLKDLGLVVEPHLYNEMMKLYVATSQNEKVPLVIRDMKQNQIMRNVLSYN 240
           NTEKAEAFM KLK LGLVV+PHLYNEMMKLYVATSQNEKVPLVI+DMKQN I+RNVLSYN
Sbjct: 185 NTEKAEAFMAKLKGLGLVVDPHLYNEMMKLYVATSQNEKVPLVIKDMKQNHILRNVLSYN 244

Query: 241 LWMNACSEMYGVGSVEWVHKEMLKDENVQVGWSTLSTLAKVYIEAGVVEKAFSALKDAEK 300
           LWMNACSE+YGV SVE V++EML D+NVQVGWSTLSTLAKVY +AG+V KAF+ALK AEK
Sbjct: 245 LWMNACSELYGVRSVELVYEEMLSDKNVQVGWSTLSTLAKVYTQAGLVHKAFAALKAAEK 304

Query: 301 KLSPCNRLGYFFLITLYASLNNKEGVLRLWRASKAVIGRPTCANYMCLLLCLVKLGEIEE 360
           KLS  NRLGYFFLITLYA+LN+KEGVLR+W ASKAV  RPTCANYMC+LLCLVKLG+I E
Sbjct: 305 KLSSGNRLGYFFLITLYATLNDKEGVLRVWTASKAVSDRPTCANYMCILLCLVKLGDINE 364

Query: 361 AEKKFKEWELSCRNYDIRVSNVLLGAYVRNGLLEKAELLHLRTLERGGSPNYKTWEILME 420
           AEK FKEWEL CRNYDIRVSNVLLGAYVRNGLLEKAE LH+ TLERGG+PNYKTWEILME
Sbjct: 365 AEKIFKEWELDCRNYDIRVSNVLLGAYVRNGLLEKAESLHMHTLERGGTPNYKTWEILME 424

Query: 421 GWMKRQNMDRAIDSLKKDYDLLKHH 445
           G ++ QN+DR I+ L+ ++  LK H
Sbjct: 425 GCVRSQNLDRGINFLENNFAGLKCH 447

BLAST of MC08g0225 vs. NCBI nr
Match: KAG7029654.1 (Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 683 bits (1762), Expect = 6.75e-244
Identity = 345/438 (78.77%), Postives = 392/438 (89.50%), Query Frame = 0

Query: 1   LGAPEIAARTESMMRTYLRSGAGRVHFLLEAWRPISSLISPSPVNSFCTISAVSPKTLKY 60
           LGA E+AART+S+M+T LRSGAGR+HFLL AWRPISS    SPV+S   ISAVSPKTL+ 
Sbjct: 5   LGASEVAARTKSVMKTTLRSGAGRIHFLLRAWRPISS--HTSPVDSSDIISAVSPKTLQK 64

Query: 61  GGDDDNFKRESKKIKLPRSSVTTVLQSGNRETQRISITKLRKVTKKLCKSKRFASALEVL 120
           GGD+ NFK++  K+K PRSSVTTVLQSGN E QR SITKLR+V KKLCKSKRF  ALE L
Sbjct: 65  GGDNGNFKQDFMKLKQPRSSVTTVLQSGNSEAQRTSITKLRRVVKKLCKSKRFERALEAL 124

Query: 121 MLMETRNKLRMSPADHALRLELTIKLHGLLEAEEYFSKLPNIASQKASSLPLLHGYVKDR 180
           MLMET+NK+RMSPADHALRLELTIK+HGLL+AEE+F++LPNIASQKASSL LLHGYVK++
Sbjct: 125 MLMETQNKIRMSPADHALRLELTIKVHGLLKAEEHFNQLPNIASQKASSLLLLHGYVKEK 184

Query: 181 NTEKAEAFMMKLKDLGLVVEPHLYNEMMKLYVATSQNEKVPLVIRDMKQNQIMRNVLSYN 240
           NTEKAEAFM KLK LGLVV+PHLYNEMMKLYVATSQNEKVPLVI+DMKQN I+RNVLSYN
Sbjct: 185 NTEKAEAFMAKLKGLGLVVDPHLYNEMMKLYVATSQNEKVPLVIKDMKQNHILRNVLSYN 244

Query: 241 LWMNACSEMYGVGSVEWVHKEMLKDENVQVGWSTLSTLAKVYIEAGVVEKAFSALKDAEK 300
           LWMNACSE+YGV SVE V++EML D+NVQVGWSTLSTLAKVY +AG+V KAF+ALK AEK
Sbjct: 245 LWMNACSELYGVRSVELVYEEMLSDKNVQVGWSTLSTLAKVYTQAGLVHKAFAALKAAEK 304

Query: 301 KLSPCNRLGYFFLITLYASLNNKEGVLRLWRASKAVIGRPTCANYMCLLLCLVKLGEIEE 360
           KLS  NRLGYFFLITLYA+LN+KEGVLR+W ASKAV  RPTCANYMC+LLCLVKLG+I E
Sbjct: 305 KLSSGNRLGYFFLITLYATLNDKEGVLRVWTASKAVSDRPTCANYMCILLCLVKLGDINE 364

Query: 361 AEKKFKEWELSCRNYDIRVSNVLLGAYVRNGLLEKAELLHLRTLERGGSPNYKTWEILME 420
           AEK FKEWEL CRNYDIRVSNVLLGAYVRNGLLEKAE LH+ TLERGG+PNYKTWEILME
Sbjct: 365 AEKIFKEWELDCRNYDIRVSNVLLGAYVRNGLLEKAESLHMHTLERGGTPNYKTWEILME 424

Query: 421 GWMKRQNMDRAIDSLKKD 438
           G ++ QN+DR  ++++++
Sbjct: 425 GCVRSQNLDRGSNNVEEN 440

BLAST of MC08g0225 vs. ExPASy TrEMBL
Match: A0A6J1BVG4 (pentatricopeptide repeat-containing protein At5g27460-like OS=Momordica charantia OX=3673 GN=LOC111005082 PE=4 SV=1)

HSP 1 Score: 867 bits (2241), Expect = 0.0
Identity = 435/435 (100.00%), Postives = 435/435 (100.00%), Query Frame = 0

Query: 13  MMRTYLRSGAGRVHFLLEAWRPISSLISPSPVNSFCTISAVSPKTLKYGGDDDNFKRESK 72
           MMRTYLRSGAGRVHFLLEAWRPISSLISPSPVNSFCTISAVSPKTLKYGGDDDNFKRESK
Sbjct: 1   MMRTYLRSGAGRVHFLLEAWRPISSLISPSPVNSFCTISAVSPKTLKYGGDDDNFKRESK 60

Query: 73  KIKLPRSSVTTVLQSGNRETQRISITKLRKVTKKLCKSKRFASALEVLMLMETRNKLRMS 132
           KIKLPRSSVTTVLQSGNRETQRISITKLRKVTKKLCKSKRFASALEVLMLMETRNKLRMS
Sbjct: 61  KIKLPRSSVTTVLQSGNRETQRISITKLRKVTKKLCKSKRFASALEVLMLMETRNKLRMS 120

Query: 133 PADHALRLELTIKLHGLLEAEEYFSKLPNIASQKASSLPLLHGYVKDRNTEKAEAFMMKL 192
           PADHALRLELTIKLHGLLEAEEYFSKLPNIASQKASSLPLLHGYVKDRNTEKAEAFMMKL
Sbjct: 121 PADHALRLELTIKLHGLLEAEEYFSKLPNIASQKASSLPLLHGYVKDRNTEKAEAFMMKL 180

Query: 193 KDLGLVVEPHLYNEMMKLYVATSQNEKVPLVIRDMKQNQIMRNVLSYNLWMNACSEMYGV 252
           KDLGLVVEPHLYNEMMKLYVATSQNEKVPLVIRDMKQNQIMRNVLSYNLWMNACSEMYGV
Sbjct: 181 KDLGLVVEPHLYNEMMKLYVATSQNEKVPLVIRDMKQNQIMRNVLSYNLWMNACSEMYGV 240

Query: 253 GSVEWVHKEMLKDENVQVGWSTLSTLAKVYIEAGVVEKAFSALKDAEKKLSPCNRLGYFF 312
           GSVEWVHKEMLKDENVQVGWSTLSTLAKVYIEAGVVEKAFSALKDAEKKLSPCNRLGYFF
Sbjct: 241 GSVEWVHKEMLKDENVQVGWSTLSTLAKVYIEAGVVEKAFSALKDAEKKLSPCNRLGYFF 300

Query: 313 LITLYASLNNKEGVLRLWRASKAVIGRPTCANYMCLLLCLVKLGEIEEAEKKFKEWELSC 372
           LITLYASLNNKEGVLRLWRASKAVIGRPTCANYMCLLLCLVKLGEIEEAEKKFKEWELSC
Sbjct: 301 LITLYASLNNKEGVLRLWRASKAVIGRPTCANYMCLLLCLVKLGEIEEAEKKFKEWELSC 360

Query: 373 RNYDIRVSNVLLGAYVRNGLLEKAELLHLRTLERGGSPNYKTWEILMEGWMKRQNMDRAI 432
           RNYDIRVSNVLLGAYVRNGLLEKAELLHLRTLERGGSPNYKTWEILMEGWMKRQNMDRAI
Sbjct: 361 RNYDIRVSNVLLGAYVRNGLLEKAELLHLRTLERGGSPNYKTWEILMEGWMKRQNMDRAI 420

Query: 433 DSLKKDYDLLKHHPG 447
           DSLKKDYDLLKHHPG
Sbjct: 421 DSLKKDYDLLKHHPG 435

BLAST of MC08g0225 vs. ExPASy TrEMBL
Match: A0A6J1KAM1 (pentatricopeptide repeat-containing protein At5g27460 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492138 PE=4 SV=1)

HSP 1 Score: 693 bits (1788), Expect = 1.73e-248
Identity = 350/445 (78.65%), Postives = 397/445 (89.21%), Query Frame = 0

Query: 1   LGAPEIAARTESMMRTYLRSGAGRVHFLLEAWRPISSLISPSPVNSFCTISAVSPKTLKY 60
           LGA E+AART+S+M+T LRSG GR+HFLL AWRPISS    SPV+S   ISAVSPKTL+ 
Sbjct: 5   LGASEVAARTKSVMKTTLRSGTGRIHFLLRAWRPISS--HTSPVDSSDIISAVSPKTLQK 64

Query: 61  GGDDDNFKRESKKIKLPRSSVTTVLQSGNRETQRISITKLRKVTKKLCKSKRFASALEVL 120
           GGD+ NFK++  K+K PRSSVTTVLQSGN E QR SITKLR+V KKLCKSKRF  ALEVL
Sbjct: 65  GGDNGNFKQDFMKLKQPRSSVTTVLQSGNSEAQRTSITKLRRVVKKLCKSKRFERALEVL 124

Query: 121 MLMETRNKLRMSPADHALRLELTIKLHGLLEAEEYFSKLPNIASQKASSLPLLHGYVKDR 180
           MLMET+NK+RMSPADHALRLELTIK+HGLL+AEE+F++LPNIASQKASSL LLHGYVK++
Sbjct: 125 MLMETQNKIRMSPADHALRLELTIKVHGLLKAEEHFNQLPNIASQKASSLLLLHGYVKEQ 184

Query: 181 NTEKAEAFMMKLKDLGLVVEPHLYNEMMKLYVATSQNEKVPLVIRDMKQNQIMRNVLSYN 240
           NTEKAEAFM KLK LGLVV+PHLYNEMMKLYVATSQNEKVPLVI+DMKQNQI+RNVLSYN
Sbjct: 185 NTEKAEAFMAKLKGLGLVVDPHLYNEMMKLYVATSQNEKVPLVIKDMKQNQILRNVLSYN 244

Query: 241 LWMNACSEMYGVGSVEWVHKEMLKDENVQVGWSTLSTLAKVYIEAGVVEKAFSALKDAEK 300
           LWMNACSE+YGVGSVE V++EML D+NVQVGWSTLSTLAKVY +AG+V KAF+AL++AEK
Sbjct: 245 LWMNACSELYGVGSVELVYEEMLSDKNVQVGWSTLSTLAKVYTQAGLVHKAFAALREAEK 304

Query: 301 KLSPCNRLGYFFLITLYASLNNKEGVLRLWRASKAVIGRPTCANYMCLLLCLVKLGEIEE 360
           KLS  NRLGYFFLITLYA+LN+KEGVLR+W ASKAV  RPTCANYMC+LLCLVKLG+I E
Sbjct: 305 KLSSGNRLGYFFLITLYATLNDKEGVLRVWTASKAVSDRPTCANYMCILLCLVKLGDINE 364

Query: 361 AEKKFKEWELSCRNYDIRVSNVLLGAYVRNGLLEKAELLHLRTLERGGSPNYKTWEILME 420
           AEK FKEWEL CRNYDIRVSNVLLGAYVRNGLL++AE LH+ TLERGG+PNYKTWEILME
Sbjct: 365 AEKIFKEWELDCRNYDIRVSNVLLGAYVRNGLLDQAESLHMHTLERGGTPNYKTWEILME 424

Query: 421 GWMKRQNMDRAIDSLKKDYDLLKHH 445
           G ++ QN+DR I+ L  D+  LK H
Sbjct: 425 GCVRSQNLDRGINFLNNDFAGLKCH 447

BLAST of MC08g0225 vs. ExPASy TrEMBL
Match: A0A6J1HF62 (pentatricopeptide repeat-containing protein At5g27460 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462481 PE=4 SV=1)

HSP 1 Score: 682 bits (1759), Expect = 4.48e-244
Identity = 346/445 (77.75%), Postives = 393/445 (88.31%), Query Frame = 0

Query: 1   LGAPEIAARTESMMRTYLRSGAGRVHFLLEAWRPISSLISPSPVNSFCTISAVSPKTLKY 60
           LGA E+AART+S+M+T LRSG GR++FLL AWRPISS    SPV+S   ISAVSPKTL+ 
Sbjct: 5   LGASEVAARTKSVMKTTLRSGTGRINFLLRAWRPISS--HTSPVDSSDIISAVSPKTLQK 64

Query: 61  GGDDDNFKRESKKIKLPRSSVTTVLQSGNRETQRISITKLRKVTKKLCKSKRFASALEVL 120
           GGD+ NFK++  K+K PRSSVTTVLQSGN E QR SITKLR+V KKLCK KRF  ALEVL
Sbjct: 65  GGDNGNFKQDFMKLKQPRSSVTTVLQSGNSEAQRTSITKLRRVVKKLCKPKRFERALEVL 124

Query: 121 MLMETRNKLRMSPADHALRLELTIKLHGLLEAEEYFSKLPNIASQKASSLPLLHGYVKDR 180
           MLMET+NK+RMSPADHALRLELTIK+HGLL+AEE+F++LPNIASQKASSL LLHGYVK++
Sbjct: 125 MLMETQNKIRMSPADHALRLELTIKVHGLLKAEEHFNQLPNIASQKASSLLLLHGYVKEK 184

Query: 181 NTEKAEAFMMKLKDLGLVVEPHLYNEMMKLYVATSQNEKVPLVIRDMKQNQIMRNVLSYN 240
           NTEKAEAFM KLK LGLVV+PHLYNEMMKLYVATSQNEKVPLVI+DMKQN I+RNVLSYN
Sbjct: 185 NTEKAEAFMAKLKGLGLVVDPHLYNEMMKLYVATSQNEKVPLVIKDMKQNHILRNVLSYN 244

Query: 241 LWMNACSEMYGVGSVEWVHKEMLKDENVQVGWSTLSTLAKVYIEAGVVEKAFSALKDAEK 300
           LWMNACSE+YG  SVE V++EML D+NVQVGWSTLSTLAKVY +AG+V KAF+ALK AEK
Sbjct: 245 LWMNACSELYGFRSVELVYEEMLSDKNVQVGWSTLSTLAKVYTQAGLVHKAFAALKAAEK 304

Query: 301 KLSPCNRLGYFFLITLYASLNNKEGVLRLWRASKAVIGRPTCANYMCLLLCLVKLGEIEE 360
           KLS  NRLGYFFLITLYA+LN+KEGVLR+W ASKAV  RPTCANYMC+LLCLVKLG++ E
Sbjct: 305 KLSSGNRLGYFFLITLYATLNDKEGVLRVWTASKAVSDRPTCANYMCILLCLVKLGDLNE 364

Query: 361 AEKKFKEWELSCRNYDIRVSNVLLGAYVRNGLLEKAELLHLRTLERGGSPNYKTWEILME 420
           AEK FKEWEL CRNYDIRVSNVLLGAYVRNGLLEKAE LH+ TLERGG+PNYKTWEILME
Sbjct: 365 AEKIFKEWELDCRNYDIRVSNVLLGAYVRNGLLEKAESLHMHTLERGGTPNYKTWEILME 424

Query: 421 GWMKRQNMDRAIDSLKKDYDLLKHH 445
           G ++ QN+DR I+ L+ ++  LK H
Sbjct: 425 GCVRSQNLDRGINFLENNFAGLKCH 447

BLAST of MC08g0225 vs. ExPASy TrEMBL
Match: A0A5A7VI21 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004540 PE=4 SV=1)

HSP 1 Score: 656 bits (1693), Expect = 4.60e-234
Identity = 334/436 (76.61%), Postives = 379/436 (86.93%), Query Frame = 0

Query: 1   LGAPEIAARTESMMRTYLRSGAGRVHFLLEAWRPISSLISPSPVNSFCTISAVSPKTLKY 60
           L A E+AARTE +M+  LRSG GR++FLL+AWR ISSL SP  V+S  T S VS K L+ 
Sbjct: 5   LRALEVAARTEPVMKMPLRSGTGRIYFLLQAWRSISSLTSP--VDSLATNSVVSSKILQN 64

Query: 61  GGDDDNFKRESKKIKLPRSSVTTVLQSGNRETQRISITKLRKVTKKLCKSKRFASALEVL 120
           GGDD NFK+E +K+KLPR SV TVLQSGN E QR SITKLR V KK CK+KRF  AL+ L
Sbjct: 65  GGDDGNFKQEFRKLKLPRRSVNTVLQSGNCEAQRTSITKLRCVVKKFCKTKRFERALKAL 124

Query: 121 MLMETRNKLRMSPADHALRLELTIKLHGLLEAEEYFSKLPNIASQKASSLPLLHGYVKDR 180
           MLMETR+  RM PA++ALRLELTIK HGLL+AEEYF++LP IASQKASSLPLLHGYVK+R
Sbjct: 125 MLMETRDNFRMYPAEYALRLELTIKAHGLLKAEEYFNQLPTIASQKASSLPLLHGYVKER 184

Query: 181 NTEKAEAFMMKLKDLGLVVEPHLYNEMMKLYVATSQNEKVPLVIRDMKQNQIMRNVLSYN 240
           NTEKAEAFM+KL+DLGLVV  HLYNEMMKLYVAT QNEKVPLVI+DMKQNQI RNVLSYN
Sbjct: 185 NTEKAEAFMVKLRDLGLVVNHHLYNEMMKLYVATYQNEKVPLVIKDMKQNQIPRNVLSYN 244

Query: 241 LWMNACSEMYGVGSVEWVHKEMLKDENVQVGWSTLSTLAKVYIEAGVVEKAFSALKDAEK 300
           LWMNACS++YGV S+E V +EML D+NVQVGWST+ TLAKVYI+ G+VEKAF+ALK+AEK
Sbjct: 245 LWMNACSQVYGVRSIELVFEEMLTDKNVQVGWSTMCTLAKVYIQEGLVEKAFAALKEAEK 304

Query: 301 KLSPCNRLGYFFLITLYASLNNKEGVLRLWRASKAVIGRPTCANYMCLLLCLVKLGEIEE 360
           KLSPC RLGYFFLITLYASL +KEGVLR+WRASKAV G PTCANYMC+LLCLVKLGE+++
Sbjct: 305 KLSPCKRLGYFFLITLYASLKDKEGVLRVWRASKAVSGNPTCANYMCILLCLVKLGEMDK 364

Query: 361 AEKKFKEWELSCRNYDIRVSNVLLGAYVRNGLLEKAELLHLRTLERGGSPNYKTWEILME 420
           AEK FKEWE +CRNYDIRVSNVLLGAYVRNGLLEKAE LH  TL RGGSPNYKTWEILME
Sbjct: 365 AEKVFKEWEFNCRNYDIRVSNVLLGAYVRNGLLEKAESLHRHTLGRGGSPNYKTWEILME 424

Query: 421 GWMK-RQNMDRAIDSL 435
           GW++ +QN+DRAI+ L
Sbjct: 425 GWVRSQQNVDRAINFL 438

BLAST of MC08g0225 vs. ExPASy TrEMBL
Match: A0A1S3BBE1 (pentatricopeptide repeat-containing protein At5g27460 OS=Cucumis melo OX=3656 GN=LOC103488093 PE=4 SV=1)

HSP 1 Score: 656 bits (1693), Expect = 4.60e-234
Identity = 334/436 (76.61%), Postives = 379/436 (86.93%), Query Frame = 0

Query: 1   LGAPEIAARTESMMRTYLRSGAGRVHFLLEAWRPISSLISPSPVNSFCTISAVSPKTLKY 60
           L A E+AARTE +M+  LRSG GR++FLL+AWR ISSL SP  V+S  T S VS K L+ 
Sbjct: 5   LRALEVAARTEPVMKMPLRSGTGRIYFLLQAWRSISSLTSP--VDSLATNSVVSSKILQN 64

Query: 61  GGDDDNFKRESKKIKLPRSSVTTVLQSGNRETQRISITKLRKVTKKLCKSKRFASALEVL 120
           GGDD NFK+E +K+KLPR SV TVLQSGN E QR SITKLR V KK CK+KRF  AL+ L
Sbjct: 65  GGDDGNFKQEFRKLKLPRRSVNTVLQSGNCEAQRTSITKLRCVVKKFCKTKRFERALKAL 124

Query: 121 MLMETRNKLRMSPADHALRLELTIKLHGLLEAEEYFSKLPNIASQKASSLPLLHGYVKDR 180
           MLMETR+  RM PA++ALRLELTIK HGLL+AEEYF++LP IASQKASSLPLLHGYVK+R
Sbjct: 125 MLMETRDNFRMYPAEYALRLELTIKAHGLLKAEEYFNQLPTIASQKASSLPLLHGYVKER 184

Query: 181 NTEKAEAFMMKLKDLGLVVEPHLYNEMMKLYVATSQNEKVPLVIRDMKQNQIMRNVLSYN 240
           NTEKAEAFM+KL+DLGLVV  HLYNEMMKLYVAT QNEKVPLVI+DMKQNQI RNVLSYN
Sbjct: 185 NTEKAEAFMVKLRDLGLVVNHHLYNEMMKLYVATYQNEKVPLVIKDMKQNQIPRNVLSYN 244

Query: 241 LWMNACSEMYGVGSVEWVHKEMLKDENVQVGWSTLSTLAKVYIEAGVVEKAFSALKDAEK 300
           LWMNACS++YGV S+E V +EML D+NVQVGWST+ TLAKVYI+ G+VEKAF+ALK+AEK
Sbjct: 245 LWMNACSQVYGVRSIELVFEEMLTDKNVQVGWSTMCTLAKVYIQEGLVEKAFAALKEAEK 304

Query: 301 KLSPCNRLGYFFLITLYASLNNKEGVLRLWRASKAVIGRPTCANYMCLLLCLVKLGEIEE 360
           KLSPC RLGYFFLITLYASL +KEGVLR+WRASKAV G PTCANYMC+LLCLVKLGE+++
Sbjct: 305 KLSPCKRLGYFFLITLYASLKDKEGVLRVWRASKAVSGNPTCANYMCILLCLVKLGEMDK 364

Query: 361 AEKKFKEWELSCRNYDIRVSNVLLGAYVRNGLLEKAELLHLRTLERGGSPNYKTWEILME 420
           AEK FKEWE +CRNYDIRVSNVLLGAYVRNGLLEKAE LH  TL RGGSPNYKTWEILME
Sbjct: 365 AEKVFKEWEFNCRNYDIRVSNVLLGAYVRNGLLEKAESLHRHTLGRGGSPNYKTWEILME 424

Query: 421 GWMK-RQNMDRAIDSL 435
           GW++ +QN+DRAI+ L
Sbjct: 425 GWVRSQQNVDRAINFL 438

BLAST of MC08g0225 vs. TAIR 10
Match: AT5G27460.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 409.8 bits (1052), Expect = 2.7e-114
Identity = 210/371 (56.60%), Postives = 275/371 (74.12%), Query Frame = 0

Query: 77  PRSSVTTVLQSGNRETQRISITKLRKVTKKLCKSKRFASALEVLMLMETRNKLRMSPADH 136
           PR SVT++LQ        +S+++LR ++K+L +S R+  AL+++  ME +  +  S  D 
Sbjct: 50  PRRSVTSLLQERIDSGHAVSLSELRLISKRLIRSNRYDLALQMMEWMENQKDIEFSVYDI 109

Query: 137 ALRLELTIKLHGLLEAEEYFSKL----PNIASQKASSLPLLHGYVKDRNTEKAEAFMMKL 196
           ALRL+L IK HGL + EEYF KL     ++   K++ LPLL  YVK++  ++AEA M KL
Sbjct: 110 ALRLDLIIKTHGLKQGEEYFEKLLHSSVSMRVAKSAYLPLLRAYVKNKMVKEAEALMEKL 169

Query: 197 KDLGLVVEPHLYNEMMKLYVATSQNEKVPLVIRDMKQNQIMRNVLSYNLWMNACSEMYGV 256
             LG +V PH +NEMMKLY A+ Q EKV +V+  MK N+I RNVLSYNLWMNAC E+ GV
Sbjct: 170 NGLGFLVTPHPFNEMMKLYEASGQYEKVVMVVSMMKGNKIPRNVLSYNLWMNACCEVSGV 229

Query: 257 GSVEWVHKEMLKDENVQVGWSTLSTLAKVYIEAGVVEKAFSALKDAEKKLSPCNRLGYFF 316
            +VE V+KEM+ D++V+VGWS+L TLA VYI++G  EKA   L+DAEK L+  NRLGYFF
Sbjct: 230 AAVETVYKEMVGDKSVEVGWSSLCTLANVYIKSGFDEKARLVLEDAEKMLNRSNRLGYFF 289

Query: 317 LITLYASLNNKEGVLRLWRASKAVIGRPTCANYMCLLLCLVKLGEIEEAEKKFKEWELSC 376
           LITLYASL NKEGV+RLW  SK+V GR +C NY+C+L  LVK G++EEAE+ F EWE  C
Sbjct: 290 LITLYASLGNKEGVVRLWEVSKSVCGRISCVNYICVLSSLVKTGDLEEAERVFSEWEAQC 349

Query: 377 RNYDIRVSNVLLGAYVRNGLLEKAELLHLRTLERGGSPNYKTWEILMEGWMKRQNMDRAI 436
            NYD+RVSNVLLGAYVRNG + KAE LH   LERGG+PNYKTWEILMEGW+K +NM++AI
Sbjct: 350 FNYDVRVSNVLLGAYVRNGEIRKAESLHGCVLERGGTPNYKTWEILMEGWVKCENMEKAI 409

Query: 437 DSLKKDYDLLK 444
           D++ + + L++
Sbjct: 410 DAMHQVFVLMR 420

BLAST of MC08g0225 vs. TAIR 10
Match: AT1G02150.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 239.6 bits (610), Expect = 4.9e-63
Identity = 145/426 (34.04%), Postives = 238/426 (55.87%), Query Frame = 0

Query: 28  LLEAWRPISSL--ISPSPVNSFCTISAVSPKTLKYGGDDDNFKRES--------KKIKL- 87
           LL    P+ S+  +S       C+IS V      YG    +++R          KKI L 
Sbjct: 23  LLRCRSPVVSVAALSKKTAAIVCSISQV------YGYGTVDYERRPIVQWNAIYKKISLM 82

Query: 88  --PRSSVTTVLQSGNRETQRISITKLRKVTKKLCKSKRFASALEVLMLMETR-NKLRMSP 147
             P     +VL    +  ++++  +L +V K+L K KR   ALEV   M  R  + R+S 
Sbjct: 83  EKPELGAASVLNQWEKAGRKLTKWELCRVVKELRKYKRANQALEVYDWMNNRGERFRLSA 142

Query: 148 ADHALRLELTIKLHGLLEAEEYFSKLPNIASQKASSLPLLHGYVKDRNTEKAEAFMMKLK 207
           +D A++L+L  K+ G+ +AEE+F +LP     +     LL+ YV+ ++ EKAEA +  ++
Sbjct: 143 SDAAIQLDLIGKVRGIPDAEEFFLQLPENFKDRRVYGSLLNAYVRAKSREKAEALLNTMR 202

Query: 208 DLGLVVEPHLYNEMMKLYVATSQNEKVPLVIRDMKQNQIMRNVLSYNLWMNACSEMYGVG 267
           D G  + P  +N MM LY+   + +KV  ++ +MKQ  I  ++ SYN+W+++C  +  V 
Sbjct: 203 DKGYALHPLPFNVMMTLYMNLREYDKVDAMVFEMKQKDIRLDIYSYNIWLSSCGSLGSVE 262

Query: 268 SVEWVHKEMLKDENVQVGWSTLSTLAKVYIEAGVVEKAFSALKDAEKKLSPCNRLGYFFL 327
            +E V+++M  D ++   W+T ST+A +YI+ G  EKA  AL+  E +++  NR+ Y +L
Sbjct: 263 KMELVYQQMKSDVSIYPNWTTFSTMATMYIKMGETEKAEDALRKVEARITGRNRIPYHYL 322

Query: 328 ITLYASLNNKEGVLRLWRASKAVIGRPTCANYMCLLLCLVKLGEIEEAEKKFKEWELSCR 387
           ++LY SL NK+ + R+W   K+V+       Y  L+  LV++G+IE AEK ++EW     
Sbjct: 323 LSLYGSLGNKKELYRVWHVYKSVVPSIPNLGYHALVSSLVRMGDIEGAEKVYEEWLPVKS 382

Query: 388 NYDIRVSNVLLGAYVRNGLLEKAELLHLRTLERGGSPNYKTWEILMEGWMKRQNMDRAID 440
           +YD R+ N+L+ AYV+N  LE AE L    +E GG P+  TWEIL  G  +++ +  A+ 
Sbjct: 383 SYDPRIPNLLMNAYVKNDQLETAEGLFDHMVEMGGKPSSSTWEILAVGHTRKRCISEALT 442

BLAST of MC08g0225 vs. TAIR 10
Match: AT1G60770.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 229.6 bits (584), Expect = 5.1e-60
Identity = 125/343 (36.44%), Postives = 195/343 (56.85%), Query Frame = 0

Query: 105 KKLCKSKRFASALEVLMLMETRNKLRMSPADHALRLELTIKLHGLLEAEEYFSKLPNIAS 164
           KKL     +  AL++  +ME R  +  + +D A+ L+L  K   +   E YF  LP  + 
Sbjct: 63  KKLRNRGLYYPALKLSEVMEERG-MNKTVSDQAIHLDLVAKAREITAGENYFVDLPETSK 122

Query: 165 QKASSLPLLHGYVKDRNTEKAEAFMMKLKDLGLVVEPHLYNEMMKLYVATSQNEKVPLVI 224
            + +   LL+ Y K+  TEKAE  + K+K+L +      YN +M LY  T + EKVP +I
Sbjct: 123 TELTYGSLLNCYCKELLTEKAEGLLNKMKELNITPSSMSYNSLMTLYTKTGETEKVPAMI 182

Query: 225 RDMKQNQIMRNVLSYNLWMNACSEMYGVGSVEWVHKEMLKDENVQVGWSTLSTLAKVYIE 284
           +++K   +M +  +YN+WM A +    +  VE V +EM +D  V   W+T S +A +Y++
Sbjct: 183 QELKAENVMPDSYTYNVWMRALAATNDISGVERVIEEMNRDGRVAPDWTTYSNMASIYVD 242

Query: 285 AGVVEKAFSALKDAEKKLSPCNRLGYFFLITLYASLNNKEGVLRLWRASKAVIGRPTCAN 344
           AG+ +KA  AL++ E K +  +   Y FLITLY  L     V R+WR+ +  I + +   
Sbjct: 243 AGLSQKAEKALQELEMKNTQRDFTAYQFLITLYGRLGKLTEVYRIWRSLRLAIPKTSNVA 302

Query: 345 YMCLLLCLVKLGEIEEAEKKFKEWELSCRNYDIRVSNVLLGAYVRNGLLEKAELLHLRTL 404
           Y+ ++  LVKL ++  AE  FKEW+ +C  YDIR+ NVL+GAY + GL++KA  L  +  
Sbjct: 303 YLNMIQVLVKLNDLPGAETLFKEWQANCSTYDIRIVNVLIGAYAQEGLIQKANELKEKAP 362

Query: 405 ERGGSPNYKTWEILMEGWMKRQNMDRAIDSLKKDYDLLKHHPG 448
            RGG  N KTWEI M+ ++K  +M RA++ + K   + K   G
Sbjct: 363 RRGGKLNAKTWEIFMDYYVKSGDMARALECMSKAVSIGKGDGG 404

BLAST of MC08g0225 vs. TAIR 10
Match: AT4G02820.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 217.6 bits (553), Expect = 2.0e-56
Identity = 126/392 (32.14%), Postives = 219/392 (55.87%), Query Frame = 0

Query: 52  AVSPKTLKYGGDDDNFKRESKKIKLPRSSVTTVLQSGNRETQRISITKLRKVTKKLCKSK 111
           + + K    GG D    R    +   RS+V T+ +    E   +   +L ++ ++L K K
Sbjct: 49  SANKKETVVGGRDTLGGRLLSLVYTKRSAVVTI-RKWKEEGHSVRKYELNRIVRELRKIK 108

Query: 112 RFASALEVLMLMETRNKLRMSPADHALRLELTIKLHGLLEAEEYFSKLPNIASQKASSLP 171
           R+  ALE+   M  +  +++   D+A+ L+L  K+ GL  AE++F  +P+     A+   
Sbjct: 109 RYKHALEICEWMVVQEDIKLQAGDYAVHLDLISKIRGLNSAEKFFEDMPDQMRGHAACTS 168

Query: 172 LLHGYVKDRNTEKAEAFMMKLKDLGLVVEPHLYNEMMKLYVATSQNEKVPLVIRDMKQNQ 231
           LLH YV+++ ++KAEA   K+ + G +     YN M+ +Y++  Q EKVP++I+++K  +
Sbjct: 169 LLHSYVQNKLSDKAEALFEKMGECGFLKSCLPYNHMLSMYISRGQFEKVPVLIKELK-IR 228

Query: 232 IMRNVLSYNLWMNACSEMYGVGSVEWVHKEMLKDENVQVGWSTLSTLAKVYIEAGVVEKA 291
              ++++YNLW+ A +    V   E V+ +  K+E +   W T S L  +Y +   VEKA
Sbjct: 229 TSPDIVTYNLWLTAFASGNDVEGAEKVYLK-AKEEKLNPDWVTYSVLTNLYAKTDNVEKA 288

Query: 292 FSALKDAEKKLSPCNRLGYFFLITLYASLNNKEGVLRLWRASKAVIGRPTCANYMCLLLC 351
             ALK+ EK +S  NR+ Y  LI+L+A+L +K+GV   W+  K+   +   A Y+ ++  
Sbjct: 289 RLALKEMEKLVSKKNRVAYASLISLHANLGDKDGVNLTWKKVKSSFKKMNDAEYLSMISA 348

Query: 352 LVKLGEIEEAEKKFKEWELSCRNYDIRVSNVLLGAYVRNGLLEKAELLHLRTLERGGSPN 411
           +VKLGE E+A+  + EWE      D R+ N++L  Y+    +   E  + R +E+G +P+
Sbjct: 349 VVKLGEFEQAKGLYDEWESVSGTGDARIPNLILAEYMNRDEVLLGEKFYERIVEKGINPS 408

Query: 412 YKTWEILMEGWMKRQNMDRAIDSLKKDYDLLK 444
           Y TWEIL   ++KR++M++ +D   K  D +K
Sbjct: 409 YSTWEILTWAYLKRKDMEKVLDCFGKAIDSVK 437

BLAST of MC08g0225 vs. TAIR 10
Match: AT4G21705.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 204.1 bits (518), Expect = 2.3e-52
Identity = 118/360 (32.78%), Postives = 193/360 (53.61%), Query Frame = 0

Query: 77  PRSSVTTVLQSGNRETQRISITKLRKVTKKLCKSKRFASALEVLMLMETRNKLRMSPADH 136
           P+SSV   LQ+  +  +++S+ +L ++   L + KRF  ALEV   M        SP +H
Sbjct: 37  PKSSVYPELQNWVQCGKKVSVAELIRIVHDLRRRKRFLHALEVSKWMNETGVCVFSPTEH 96

Query: 137 ALRLELTIKLHGLLEAEEYFSKLPNIASQKASSLPLLHGYVKDRNTEKAEAFMMKLKDLG 196
           A+ L+L  +++G + AEEYF  L        +   LL+ YV+ +N EK+     K+K++G
Sbjct: 97  AVHLDLIGRVYGFVTAEEYFENLKEQYKNDKTYGALLNCYVRQQNVEKSLLHFEKMKEMG 156

Query: 197 LVVEPHLYNEMMKLYVATSQNEKVPLVIRDMKQNQIMRNVLSYNLWMNACSEMYGVGSVE 256
            V     YN +M LY    Q+EKVP V+ +MK+  +  +  SY + +NA   MY +  + 
Sbjct: 157 FVTSSLTYNNIMCLYTNIGQHEKVPKVLEEMKEENVAPDNYSYRICINAFGAMYDLERIG 216

Query: 257 WVHKEMLKDENVQVGWSTLSTLAKVYIEAGVVEKAFSALKDAEKKLSPCNRLGYFFLITL 316
              ++M + +++ + W+T +  AK YI+ G  ++A   LK +E +L   +  GY  LITL
Sbjct: 217 GTLRDMERRQDITMDWNTYAVAAKFYIDGGDCDRAVELLKMSENRLEKKDGEGYNHLITL 276

Query: 317 YASLNNKEGVLRLWRASKAVIGRPTCANYMCLLLCLVKLGEIEEAEKKFKEWELSCRNYD 376
           YA L  K  VLRLW   K V  R    +Y+ +L  LVK+  + EAE+   EW+ S   YD
Sbjct: 277 YARLGKKIEVLRLWDLEKDVCKRRINQDYLTVLQSLVKIDALVEAEEVLTEWKSSGNCYD 336

Query: 377 IRVSNVLLGAYVRNGLLEKAELLHLRTLERGGSPNYKTWEILMEGWMKRQNMDRAIDSLK 436
            RV N ++  Y+   + EKAE +      RG +   ++WE++   + ++  ++ A   +K
Sbjct: 337 FRVPNTVIRGYIGKSMEEKAEAMLEDLARRGKATTPESWELVATAYAEKGTLENAFKCMK 396

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3E9113.9e-11356.60Pentatricopeptide repeat-containing protein At5g27460 OS=Arabidopsis thaliana OX... [more]
Q8LPS66.9e-6234.04Pentatricopeptide repeat-containing protein At1g02150 OS=Arabidopsis thaliana OX... [more]
O227147.1e-5936.44Pentatricopeptide repeat-containing protein At1g60770 OS=Arabidopsis thaliana OX... [more]
Q9SY072.8e-5532.14Pentatricopeptide repeat-containing protein At4g02820, mitochondrial OS=Arabidop... [more]
Q84JR33.2e-5132.78Pentatricopeptide repeat-containing protein At4g21705, mitochondrial OS=Arabidop... [more]
Match NameE-valueIdentityDescription
XP_022132148.10.0100.00pentatricopeptide repeat-containing protein At5g27460-like [Momordica charantia][more]
XP_022997144.13.57e-24878.65pentatricopeptide repeat-containing protein At5g27460 isoform X1 [Cucurbita maxi... [more]
XP_023545813.15.89e-24779.10pentatricopeptide repeat-containing protein At5g27460 [Cucurbita pepo subsp. pep... [more]
KAG6598713.11.19e-24678.65Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma sub... [more]
KAG7029654.16.75e-24478.77Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma sub... [more]
Match NameE-valueIdentityDescription
A0A6J1BVG40.0100.00pentatricopeptide repeat-containing protein At5g27460-like OS=Momordica charanti... [more]
A0A6J1KAM11.73e-24878.65pentatricopeptide repeat-containing protein At5g27460 isoform X1 OS=Cucurbita ma... [more]
A0A6J1HF624.48e-24477.75pentatricopeptide repeat-containing protein At5g27460 isoform X1 OS=Cucurbita mo... [more]
A0A5A7VI214.60e-23476.61Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3BBE14.60e-23476.61pentatricopeptide repeat-containing protein At5g27460 OS=Cucumis melo OX=3656 GN... [more]
Match NameE-valueIdentityDescription
AT5G27460.12.7e-11456.60Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G02150.14.9e-6334.04Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G60770.15.1e-6036.44Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT4G02820.12.0e-5632.14Pentatricopeptide repeat (PPR) superfamily protein [more]
AT4G21705.12.3e-5232.78Tetratricopeptide repeat (TPR)-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 358..378
NoneNo IPR availablePANTHERPTHR45717OS12G0527900 PROTEINcoord: 44..444
NoneNo IPR availablePANTHERPTHR45717:SF13OS02G0796400 PROTEINcoord: 44..444
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 381..407
e-value: 0.022
score: 15.0
coord: 345..368
e-value: 0.89
score: 10.0
IPR002885Pentatricopeptide repeatPFAMPF13812PPR_3coord: 193..248
e-value: 0.0023
score: 18.0
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 376..410
score: 9.032168
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 67..252
e-value: 5.3E-10
score: 40.9
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 280..444
e-value: 2.1E-19
score: 72.1

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g0225.1MC08g0225.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005515 protein binding