MC08g0025 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g0025
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionDNA-directed RNA polymerases I, II, and III subunit RPABC5
LocationMC08: 173454 .. 174052 (+)
RNA-Seq ExpressionMC08g0025
SyntenyMC08g0025
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AGGCAGCGTCGGAACCCCGGGCTAGGGCAGAAAGCGAAGATGATCATCCCAGTACGTTGCTTCACTTGCGGAAAGGTAAGCCATGGAGAAATAAATTCAATTTTTAGTTTAACTACCTTCTACTTATACTCCTATGTGGGAGCCTCTTCTGCAAATTTGCGTTTTTTCTGTTTTCTTGTGTGCAACTATCTTTATCTTTGGTTTGCTGCTTACGAGCACGATAAATTTTTGAATTTGTAATTTGTCAGGTGATTGGAAACAAATGGGATCATTACCTTGATCTTCTACAGGCAGACTATTCTGAAGGGTACGGATGTAGCCTCCCCTTTTCTTGTCATTTTAAGTTCTCACTCTAAAATTTCTCTCTCAAATTCCTTCTGGACTTTGAAGATTGCCTGGTCAATGTACGATATCTAACCTTTAACCTTGCTATGTAAAGATGATGCTGATGCGTTTTTTCTTGTATTAGAGATGCTCTCGATGCACTGGGCTTGGTTCGCTACTGCTGTAGGCGAATGCTCATGACTCATGTAGACCTTATCGAAAAGCTTCTTAATTACAACAGTAATTTCTCTCTCTTTCTCTACATTCATGCACGA

mRNA sequence

AGGCAGCGTCGGAACCCCGGGCTAGGGCAGAAAGCGAAGATGATCATCCCAGTACGTTGCTTCACTTGCGGAAAGGTGATTGGAAACAAATGGGATCATTACCTTGATCTTCTACAGGCAGACTATTCTGAAGGAGATGCTCTCGATGCACTGGGCTTGGTTCGCTACTGCTGTAGGCGAATGCTCATGACTCATGTAGACCTTATCGAAAAGCTTCTTAATTACAACAGTAATTTCTCTCTCTTTCTCTACATTCATGCACGA

Coding sequence (CDS)

AGGCAGCGTCGGAACCCCGGGCTAGGGCAGAAAGCGAAGATGATCATCCCAGTACGTTGCTTCACTTGCGGAAAGGTGATTGGAAACAAATGGGATCATTACCTTGATCTTCTACAGGCAGACTATTCTGAAGGAGATGCTCTCGATGCACTGGGCTTGGTTCGCTACTGCTGTAGGCGAATGCTCATGACTCATGTAGACCTTATCGAAAAGCTTCTTAATTACAACAGTAATTTCTCTCTCTTTCTCTACATTCATGCACGA

Protein sequence

RQRRNPGLGQKAKMIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNSNFSLFLYIHAR
Homology
BLAST of MC08g0025 vs. ExPASy Swiss-Prot
Match: Q9SYA6 (DNA-directed RNA polymerase subunit 10-like protein OS=Arabidopsis thaliana OX=3702 GN=NRPB10L PE=1 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 8.0e-31
Identity = 59/64 (92.19%), Postives = 63/64 (98.44%), Query Frame = 0

Query: 14 MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLL 73
          MI+PVRCFTCGKVIGNKWD YL+LLQADY+EGDALDALGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIVPVRCFTCGKVIGNKWDTYLELLQADYAEGDALDALGLVRYCCRRMLMTHVDLIEKLL 60

Query: 74 NYNS 78
          NYN+
Sbjct: 61 NYNT 64

BLAST of MC08g0025 vs. ExPASy Swiss-Prot
Match: Q8LFJ6 (DNA-directed RNA polymerases II, IV and V subunit 10 OS=Arabidopsis thaliana OX=3702 GN=NRPB10 PE=1 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 4.0e-30
Identity = 59/64 (92.19%), Postives = 61/64 (95.31%), Query Frame = 0

Query: 14 MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLL 73
          MIIPVRCFTCGKVIGNKWD YLDLLQ DY+EGDALDAL LVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDQYLDLLQLDYTEGDALDALQLVRYCCRRMLMTHVDLIEKLL 60

Query: 74 NYNS 78
          NYN+
Sbjct: 61 NYNT 64

BLAST of MC08g0025 vs. ExPASy Swiss-Prot
Match: Q39290 (DNA-directed RNA polymerases I, II, and III subunit RPABC5 OS=Brassica napus OX=3708 PE=3 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 4.0e-30
Identity = 59/64 (92.19%), Postives = 61/64 (95.31%), Query Frame = 0

Query: 14 MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLL 73
          MIIPVRCFTCGKVIGNKWD YLDLLQ DY+EGDALDAL LVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDAYLDLLQLDYTEGDALDALNLVRYCCRRMLMTHVDLIEKLL 60

Query: 74 NYNS 78
          NYN+
Sbjct: 61 NYNT 64

BLAST of MC08g0025 vs. ExPASy Swiss-Prot
Match: Q55AB6 (DNA-directed RNA polymerases I, II, and III subunit rpabc5 OS=Dictyostelium discoideum OX=44689 GN=polr2l PE=3 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 4.1e-27
Identity = 55/62 (88.71%), Postives = 57/62 (91.94%), Query Frame = 0

Query: 14 MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLL 73
          MIIPVRCFTCGKVIGNKWD YL LLQ DY+EGDALDAL L RYCCRRML+THVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDAYLSLLQIDYTEGDALDALCLKRYCCRRMLLTHVDLIEKLL 60

Query: 74 NY 76
          NY
Sbjct: 61 NY 62

BLAST of MC08g0025 vs. ExPASy Swiss-Prot
Match: Q32P78 (DNA-directed RNA polymerases I, II, and III subunit RPABC5 OS=Bos taurus OX=9913 GN=POLR2L PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 7.0e-27
Identity = 52/62 (83.87%), Postives = 58/62 (93.55%), Query Frame = 0

Query: 14 MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLL 73
          MIIPVRCFTCGK++GNKW+ YL LLQA+Y+EGDALDALGL RYCCRRML+ HVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKIVGNKWEAYLGLLQAEYTEGDALDALGLKRYCCRRMLLAHVDLIEKLL 60

Query: 74 NY 76
          NY
Sbjct: 61 NY 62

BLAST of MC08g0025 vs. NCBI nr
Match: KAA0057130.1 (DNA-directed RNA polymerases I, II, and III subunit RPABC5 [Cucumis melo var. makuwa] >TYJ97111.1 DNA-directed RNA polymerases I, II, and III subunit RPABC5 [Cucumis melo var. makuwa])

HSP 1 Score: 144 bits (364), Expect = 5.16e-43
Identity = 68/71 (95.77%), Postives = 69/71 (97.18%), Query Frame = 0

Query: 14 MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLL 73
          MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLL 60

Query: 74 NYNSNFSLFLY 84
          NYNSN SL L+
Sbjct: 61 NYNSNPSLSLF 71

BLAST of MC08g0025 vs. NCBI nr
Match: KAF9825663.1 (hypothetical protein H0E87_032116, partial [Populus deltoides])

HSP 1 Score: 144 bits (364), Expect = 6.35e-43
Identity = 67/69 (97.10%), Postives = 68/69 (98.55%), Query Frame = 0

Query: 14 MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLL 73
          MIIPVRCFTCGKVIGNKWD YLDLLQADY+EGDALDALGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDTYLDLLQADYTEGDALDALGLVRYCCRRMLMTHVDLIEKLL 60

Query: 74 NYNSNFSLF 82
          NYNSNFSLF
Sbjct: 61 NYNSNFSLF 69

BLAST of MC08g0025 vs. NCBI nr
Match: KAB5564305.1 (hypothetical protein DKX38_004359 [Salix brachista])

HSP 1 Score: 144 bits (363), Expect = 3.16e-42
Identity = 67/70 (95.71%), Postives = 68/70 (97.14%), Query Frame = 0

Query: 14 MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLL 73
          MIIPVRCFTCGKVIGNKWD YLDLLQADY+EGDALDALGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDTYLDLLQADYTEGDALDALGLVRYCCRRMLMTHVDLIEKLL 60

Query: 74 NYNSNFSLFL 83
          NYNSNFS FL
Sbjct: 61 NYNSNFSFFL 70

BLAST of MC08g0025 vs. NCBI nr
Match: KAG6782578.1 (hypothetical protein POTOM_011988 [Populus tomentosa])

HSP 1 Score: 144 bits (364), Expect = 5.72e-42
Identity = 67/69 (97.10%), Postives = 68/69 (98.55%), Query Frame = 0

Query: 14 MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLL 73
          MIIPVRCFTCGKVIGNKWD YLDLLQADY+EGDALDALGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDTYLDLLQADYTEGDALDALGLVRYCCRRMLMTHVDLIEKLL 60

Query: 74 NYNSNFSLF 82
          NYNSNFSLF
Sbjct: 61 NYNSNFSLF 69

BLAST of MC08g0025 vs. NCBI nr
Match: KAF9685616.1 (hypothetical protein SADUNF_Sadunf03G0073100 [Salix dunnii])

HSP 1 Score: 142 bits (359), Expect = 5.79e-42
Identity = 66/68 (97.06%), Postives = 67/68 (98.53%), Query Frame = 0

Query: 14 MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLL 73
          MIIPVRCFTCGKVIGNKWD YLDLLQADY+EGDALDALGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDTYLDLLQADYTEGDALDALGLVRYCCRRMLMTHVDLIEKLL 60

Query: 74 NYNSNFSL 81
          NYNSNFSL
Sbjct: 61 NYNSNFSL 68

BLAST of MC08g0025 vs. ExPASy TrEMBL
Match: A0A5A7UPU3 (DNA-directed RNA polymerases I, II, and III subunit RPABC5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold371G00170 PE=3 SV=1)

HSP 1 Score: 144 bits (364), Expect = 2.50e-43
Identity = 68/71 (95.77%), Postives = 69/71 (97.18%), Query Frame = 0

Query: 14 MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLL 73
          MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLL 60

Query: 74 NYNSNFSLFLY 84
          NYNSN SL L+
Sbjct: 61 NYNSNPSLSLF 71

BLAST of MC08g0025 vs. ExPASy TrEMBL
Match: A0A5N5N9W5 (Uncharacterized protein OS=Salix brachista OX=2182728 GN=DKX38_004359 PE=3 SV=1)

HSP 1 Score: 144 bits (363), Expect = 1.53e-42
Identity = 67/70 (95.71%), Postives = 68/70 (97.14%), Query Frame = 0

Query: 14 MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLL 73
          MIIPVRCFTCGKVIGNKWD YLDLLQADY+EGDALDALGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDTYLDLLQADYTEGDALDALGLVRYCCRRMLMTHVDLIEKLL 60

Query: 74 NYNSNFSLFL 83
          NYNSNFS FL
Sbjct: 61 NYNSNFSFFL 70

BLAST of MC08g0025 vs. ExPASy TrEMBL
Match: A0A453FTM1 (Uncharacterized protein OS=Aegilops tauschii subsp. strangulata OX=200361 PE=3 SV=1)

HSP 1 Score: 143 bits (360), Expect = 2.52e-42
Identity = 65/71 (91.55%), Postives = 69/71 (97.18%), Query Frame = 0

Query: 7   GLGQKAKMIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHV 66
           G G+ +KMIIPVRCFTCGKVIGNKWDHYLDLLQADY+EGDALDALGLVRYCCRRMLMTHV
Sbjct: 38  GGGRGSKMIIPVRCFTCGKVIGNKWDHYLDLLQADYTEGDALDALGLVRYCCRRMLMTHV 97

Query: 67  DLIEKLLNYNS 77
           DLIEKLLNYN+
Sbjct: 98  DLIEKLLNYNT 108

BLAST of MC08g0025 vs. ExPASy TrEMBL
Match: I1K9Q6 (Uncharacterized protein OS=Glycine max OX=3847 GN=GLYMA_06G096400 PE=3 SV=1)

HSP 1 Score: 141 bits (355), Expect = 3.72e-42
Identity = 65/67 (97.01%), Postives = 65/67 (97.01%), Query Frame = 0

Query: 14 MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLL 73
          MIIPVRCFTCGKVIGNKWD YLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDTYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLL 60

Query: 74 NYNSNFS 80
          NYNSNF 
Sbjct: 61 NYNSNFG 67

BLAST of MC08g0025 vs. ExPASy TrEMBL
Match: T1MEG7 (Uncharacterized protein OS=Triticum urartu OX=4572 PE=3 SV=1)

HSP 1 Score: 140 bits (353), Expect = 8.40e-42
Identity = 63/66 (95.45%), Postives = 66/66 (100.00%), Query Frame = 0

Query: 12 AKMIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEK 71
          +KMIIPVRCFTCGKVIGNKWDHYLDLLQADY+EGDALDALGLVRYCCRRMLMTHVDLIEK
Sbjct: 1  SKMIIPVRCFTCGKVIGNKWDHYLDLLQADYTEGDALDALGLVRYCCRRMLMTHVDLIEK 60

Query: 72 LLNYNS 77
          LLNYN+
Sbjct: 61 LLNYNT 66

BLAST of MC08g0025 vs. TAIR 10
Match: AT1G61700.1 (RNA polymerases N / 8 kDa subunit )

HSP 1 Score: 134.0 bits (336), Expect = 5.7e-32
Identity = 59/64 (92.19%), Postives = 63/64 (98.44%), Query Frame = 0

Query: 14 MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLL 73
          MI+PVRCFTCGKVIGNKWD YL+LLQADY+EGDALDALGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIVPVRCFTCGKVIGNKWDTYLELLQADYAEGDALDALGLVRYCCRRMLMTHVDLIEKLL 60

Query: 74 NYNS 78
          NYN+
Sbjct: 61 NYNT 64

BLAST of MC08g0025 vs. TAIR 10
Match: AT1G11475.1 (RNA polymerases N / 8 kDa subunit )

HSP 1 Score: 131.7 bits (330), Expect = 2.8e-31
Identity = 59/64 (92.19%), Postives = 61/64 (95.31%), Query Frame = 0

Query: 14 MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLL 73
          MIIPVRCFTCGKVIGNKWD YLDLLQ DY+EGDALDAL LVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDQYLDLLQLDYTEGDALDALQLVRYCCRRMLMTHVDLIEKLL 60

Query: 74 NYNS 78
          NYN+
Sbjct: 61 NYNT 64

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SYA68.0e-3192.19DNA-directed RNA polymerase subunit 10-like protein OS=Arabidopsis thaliana OX=3... [more]
Q8LFJ64.0e-3092.19DNA-directed RNA polymerases II, IV and V subunit 10 OS=Arabidopsis thaliana OX=... [more]
Q392904.0e-3092.19DNA-directed RNA polymerases I, II, and III subunit RPABC5 OS=Brassica napus OX=... [more]
Q55AB64.1e-2788.71DNA-directed RNA polymerases I, II, and III subunit rpabc5 OS=Dictyostelium disc... [more]
Q32P787.0e-2783.87DNA-directed RNA polymerases I, II, and III subunit RPABC5 OS=Bos taurus OX=9913... [more]
Match NameE-valueIdentityDescription
KAA0057130.15.16e-4395.77DNA-directed RNA polymerases I, II, and III subunit RPABC5 [Cucumis melo var. ma... [more]
KAF9825663.16.35e-4397.10hypothetical protein H0E87_032116, partial [Populus deltoides][more]
KAB5564305.13.16e-4295.71hypothetical protein DKX38_004359 [Salix brachista][more]
KAG6782578.15.72e-4297.10hypothetical protein POTOM_011988 [Populus tomentosa][more]
KAF9685616.15.79e-4297.06hypothetical protein SADUNF_Sadunf03G0073100 [Salix dunnii][more]
Match NameE-valueIdentityDescription
A0A5A7UPU32.50e-4395.77DNA-directed RNA polymerases I, II, and III subunit RPABC5 OS=Cucumis melo var. ... [more]
A0A5N5N9W51.53e-4295.71Uncharacterized protein OS=Salix brachista OX=2182728 GN=DKX38_004359 PE=3 SV=1[more]
A0A453FTM12.52e-4291.55Uncharacterized protein OS=Aegilops tauschii subsp. strangulata OX=200361 PE=3 S... [more]
I1K9Q63.72e-4297.01Uncharacterized protein OS=Glycine max OX=3847 GN=GLYMA_06G096400 PE=3 SV=1[more]
T1MEG78.40e-4295.45Uncharacterized protein OS=Triticum urartu OX=4572 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G61700.15.7e-3292.19RNA polymerases N / 8 kDa subunit [more]
AT1G11475.12.8e-3192.19RNA polymerases N / 8 kDa subunit [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.10.60coord: 14..78
e-value: 2.9E-39
score: 135.0
NoneNo IPR availablePANTHERPTHR23431:SF6SUBFAMILY NOT NAMEDcoord: 14..77
IPR000268DNA-directed RNA polymerase, subunit N/Rpb10PFAMPF01194RNA_pol_Ncoord: 14..72
e-value: 9.5E-32
score: 109.1
IPR000268DNA-directed RNA polymerase, subunit N/Rpb10PANTHERPTHR23431DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5 FAMILY MEMBERcoord: 14..77
IPR000268DNA-directed RNA polymerase, subunit N/Rpb10HAMAPMF_00250RNApol_arch_Ncoord: 14..76
score: 19.33688
IPR020789RNA polymerases, subunit N, zinc binding sitePROSITEPS01112RNA_POL_N_8KDcoord: 15..24
IPR023580RNA polymerase subunit RPB10SUPERFAMILY46924RNA polymerase subunit RPB10coord: 14..76

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g0025.1MC08g0025.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006360 transcription by RNA polymerase I
biological_process GO:0006366 transcription by RNA polymerase II
biological_process GO:0042797 tRNA transcription by RNA polymerase III
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0005736 RNA polymerase I complex
cellular_component GO:0005665 RNA polymerase II, core complex
cellular_component GO:0005666 RNA polymerase III complex
molecular_function GO:0003677 DNA binding
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0008270 zinc ion binding