MC07g_new0370 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC07g_new0370
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	MC07: 14103202 .. 14105962 (-)
RNA-Seq Expression	MC07g_new0370
Synteny	MC07g_new0370

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTAATTATTACTTAAAGAACTATTTATTTATAAAATCCTTTTATTTAAATTACTTTTTTTTTTAACACAAGACTTATAGGAGGTAAAAGTATGGTCACCGACGGACAAAAGAGAGATTTTTTAATGGCCTCACGTTTCAGCTATATCCTAATTCCCTAATAGAAAAATAAAAATCCAATAAGCTCAATTATACACCTCAGAGTTGGAATCAAATGGCCGCCATTGGAACCGGAACCGAGTCACTCAGCTCCAGAAGAGCCGAGTTGGAATCGTTCGACAACTCGAAAGCCGGCGCTAAGGGGCTGTCGGATGCCGGAATCACCAAAATCCCTCCCATATTTCACTGCCAGAAACAGGATCTCAACGCCGCCGCCGAGTCGGAGCTGAGTATTCCGATCATCGACCTCCACGGCGGCGAAGAGGTGGTGATGGATCAGATCGGAAGTGCCTGCCGGAAATGGGGATTCTTCCAGGTGGTGAACCACGGGATTCCGGTGGAAGTTTTGGACGGAATGATTGACGGAATCCGTGGGTTTCACGAACAAGATTTGGAGTGGAAGAAACAATTTTACAGCCGGGATCGTGGTAAGAAAGTGGCCTATTTTTCAAATTTCGATCTGTTCCAATCAAAAGCAGCTAATTGGAAAGACACTCTTTCTTTGTCCATGGCTCCAATCCATCCCGATCCTCATGAGATACCTGAAATTTGTAGGTAAATCTTACAAACGCTTATCAATTTGGTCGTAAATGTTGTTTTTCTCTGCTCTCTTTAACCTCCCAATTTGGTCTGGTTTATCCATTTTTATTCTGAACTCCCAATTTCATTGGATTTCATTTAAGTTTAGAGAACTACGACAAATTTTAGCTTAAATGTGAAAAACGTGTTTGCAATTTTTGTTATATGATATGTTATTTAAAAATAAAATATATGTCGACGTGAGCTAAATTGAACGAGATATATATTTAAGATCAAAAGATTGGAAGTTTGAATCGGTACTTTCAAAAGTTGCTGCACATAAAAAAATAAATATCTATTTTGAATTTATGTCAACCTGAGTATAGCTGAACTAAATAAGATATATATCTGCGATGAAAAGATTAAAAGTTCGAATCAATTTCTATTTTCACCTGTTGTTGGAAAAAAAGTTATGGTATTTATGAGAAGAATGAGCTAAGAGTTTGTCTTTTTACTTTTTTTTTTTTAATTTGTGAATTTTGATTGAGTTATTTAATTATTGATTTTGTACATCAATTAATAATATTGTGCCAAACTTCGTTGAAATTAACAGAAATTAAATAAATAGTAAACATTGCCACATTTATATAAGTCTAAGTTTAGTGTCCTAAGTGGATGAAATTAAAAGTTTATGGGAAAAATTAATACAACCTATCAAGATTATGAGTAAAATGATTCTCTCTTTTTTTTTAATGAGGTATTTATAAGAAGACCTTGAACTTTTAATAAATTTTTAGACTTTAATTTTGTGTAGGGTTGACAATCTTACTTAAGGAATAGAACTCAGACCCTATCCCATGATCTTTAAATAGTAGTAGTTTTGAGTGACTGAGAAAATGCTGATGTGGAATGGCATTATTGGCAGAGAAATAGTGATTGAATACACAAAGGAAGTAAAGAAACTAGGGTGGATTCTGTTGGAGCTGATATCCAAGGCTCTTGGGCTCGAAGGTAGCCATTTGAAGGACATTGGGTGCGCCGATGGGGTCCTAATGTTGAGCCATTACTACCCACCCTGCCCTGAACCACACCTCACCATCGGCACCGCCAACCACACCGACGTTGCCTTCATCACCATACTTTTACAGGACCAAATTGGTGGCCTCCAAGTCTTGCACCACAATCGCTGGCTCCATGTTCCTCCCCTCCATGGAGCTCTTGTGATCAACCTTGGTGATTTCCTTCAGGAAAGTGCCGCCCAATTCATCTTTAAAAAAGTCTTAATACATCTCTCGAGATTTCAGTCAATTTCTATCGAGTAGATCCCTAGCTATCGTTAGTTAGGTCAACTTAACGCTTACACCTCTAAATGGATGAACCTGCTCGTAATCAACTATATTGCGTCTTAGTTAAGTAGACAAGACTTAATCTATTCGACAAAAATATTTTTTTTTTAGTACATGAACTGCATAGAAGCAATCTATAAGGTTATGAGACGATGGAACTTGAAATTTGACTTAATTTTTAATATTGTGATGGAGGAAAATAGTGAATATATGTGTTTTGCTGTTTCTAGCAGATTGTATCCAACGATGCAATGGTGAGTGTGAGCCACAGGGTGGCGGCGAAAAAGATAGGGCCAAGGGTTTCAGTAGCAAGTCAGTTTCGACCCTACGTAGGAGAGGACGTGGGAAACTCGAAAGTATATGAACCGATTAAAGAGTTGTTATCGGAAGACAACCCTCCAAAATACAAACAGGTTGATCCCAAAGAATATATTAAGCTACACGCTTCAAAAGGATTTGATGGGAGTTCAAAATTAGAGTTGTTTAAGCTGTAGAACAATGTTTCTGCAACTCAGATGCATTGTTTCGGGAGAAATAATTGTGCACTAAGTTTTTCTTTTCTTTTTTGAATATATGTATTGAAAAAATAATTATGGACAAGTTGTTTGAGATATAAAGAGAAATGCAACTTTAAGGACATTTTTTTTTTGGATTAGATGAAACATTAGCTAATTAGCTAAATGACATCCACAAATTATATATATTCTAAATCAACGACTAAAAAGTTGTTTAATTGTCT

mRNA sequence

ATGGCCGCCATTGGAACCGGAACCGAGTCACTCAGCTCCAGAAGAGCCGAGTTGGAATCGTTCGACAACTCGAAAGCCGGCGCTAAGGGGCTGTCGGATGCCGGAATCACCAAAATCCCTCCCATATTTCACTGCCAGAAACAGGATCTCAACGCCGCCGCCGAGTCGGAGCTGAGTATTCCGATCATCGACCTCCACGGCGGCGAAGAGGTGGTGATGGATCAGATCGGAAGTGCCTGCCGGAAATGGGGATTCTTCCAGGTGGTGAACCACGGGATTCCGGTGGAAGTTTTGGACGGAATGATTGACGGAATCCGTGGGTTTCACGAACAAGATTTGGAGTGGAAGAAACAATTTTACAGCCGGGATCGTGGTAAGAAAGTGGCCTATTTTTCAAATTTCGATCTGTTCCAATCAAAAGCAGCTAATTGGAAAGACACTCTTTCTTTGTCCATGGCTCCAATCCATCCCGATCCTCATGAGATACCTGAAATTTGTAGAGAAATAGTGATTGAATACACAAAGGAAGTAAAGAAACTAGGGTGGATTCTGTTGGAGCTGATATCCAAGGCTCTTGGGCTCGAAGGTAGCCATTTGAAGGACATTGGGTGCGCCGATGGGGTCCTAATGTTGAGCCATTACTACCCACCCTGCCCTGAACCACACCTCACCATCGGCACCGCCAACCACACCGACGTTGCCTTCATCACCATACTTTTACAGGACCAAATTGGTGGCCTCCAAGTCTTGCACCACAATCGCTGGCTCCATGTTCCTCCCCTCCATGGAGCTCTTGTGATCAACCTTGGTGATTTCCTTCAGGAAAGTGCCGCCCAATTCATCTTTAAAAAAGTCTTAATACATCTCTCGAGATTTCAGTCAATTTCTATCGAGTAG

Coding sequence (CDS)

ATGGCCGCCATTGGAACCGGAACCGAGTCACTCAGCTCCAGAAGAGCCGAGTTGGAATCGTTCGACAACTCGAAAGCCGGCGCTAAGGGGCTGTCGGATGCCGGAATCACCAAAATCCCTCCCATATTTCACTGCCAGAAACAGGATCTCAACGCCGCCGCCGAGTCGGAGCTGAGTATTCCGATCATCGACCTCCACGGCGGCGAAGAGGTGGTGATGGATCAGATCGGAAGTGCCTGCCGGAAATGGGGATTCTTCCAGGTGGTGAACCACGGGATTCCGGTGGAAGTTTTGGACGGAATGATTGACGGAATCCGTGGGTTTCACGAACAAGATTTGGAGTGGAAGAAACAATTTTACAGCCGGGATCGTGGTAAGAAAGTGGCCTATTTTTCAAATTTCGATCTGTTCCAATCAAAAGCAGCTAATTGGAAAGACACTCTTTCTTTGTCCATGGCTCCAATCCATCCCGATCCTCATGAGATACCTGAAATTTGTAGAGAAATAGTGATTGAATACACAAAGGAAGTAAAGAAACTAGGGTGGATTCTGTTGGAGCTGATATCCAAGGCTCTTGGGCTCGAAGGTAGCCATTTGAAGGACATTGGGTGCGCCGATGGGGTCCTAATGTTGAGCCATTACTACCCACCCTGCCCTGAACCACACCTCACCATCGGCACCGCCAACCACACCGACGTTGCCTTCATCACCATACTTTTACAGGACCAAATTGGTGGCCTCCAAGTCTTGCACCACAATCGCTGGCTCCATGTTCCTCCCCTCCATGGAGCTCTTGTGATCAACCTTGGTGATTTCCTTCAGGAAAGTGCCGCCCAATTCATCTTTAAAAAAGTCTTAATACATCTCTCGAGATTTCAGTCAATTTCTATCGAGTAG

Protein sequence

MAAIGTGTESLSSRRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNAAAESELSIPIIDLHGGEEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDRGKKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWILLELISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQIGGLQVLHHNRWLHVPPLHGALVINLGDFLQESAAQFIFKKVLIHLSRFQSISIE

Homology

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BLAST of MC07g_new0370 vs. ExPASy Swiss-Prot
Match: Q84MB3 (1-aminocyclopropane-1-carboxylate oxidase homolog 1 OS=Arabidopsis thaliana OX=3702 GN=At1g06620 PE=2 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 1.7e-85
Identity = 152/270 (56.30%), Postives = 191/270 (70.74%), Query Frame = 0


Query: 14  RRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNA---AAESELSIPIIDLHGG-- 73
           R   L++FD +K G KGL DAGIT+IP IF      L +    + S+ SIP IDL GG  
Sbjct: 13  RSTLLKAFDETKTGVKGLIDAGITEIPSIFRAPPATLTSPKPPSSSDFSIPTIDLKGGGT 72

Query: 74  ----EEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDR 133
                  ++++IG A  KWGFFQV+NHGIP++VL+ MIDGIR FHEQD E KK FYSRD 
Sbjct: 73  DSITRRSLVEKIGDAAEKWGFFQVINHGIPMDVLEKMIDGIREFHEQDTEVKKGFYSRDP 132

Query: 134 GKKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWIL 193
             K+ Y SNFDLF S AANW+DTL    AP  P P ++P  C E++IEY+KEV KLG +L
Sbjct: 133 ASKMVYSSNFDLFSSPAANWRDTLGCYTAPDPPRPEDLPATCGEMMIEYSKEVMKLGKLL 192

Query: 194 LELISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQI 253
            EL+S+ALGL  +HLKD+ C + +L+L HYYPPCP+P LT+G   H+D +F+TILLQD I
Sbjct: 193 FELLSEALGLNTNHLKDMDCTNSLLLLGHYYPPCPQPDLTLGLTKHSDNSFLTILLQDHI 252

Query: 254 GGLQVLHHNRWLHVPPLHGALVINLGDFLQ 275
           GGLQVLH   W+ VPP+ GALV+N+GD LQ
Sbjct: 253 GGLQVLHDQYWVDVPPVPGALVVNVGDLLQ 282

[Close]

BLAST of MC07g_new0370 vs. ExPASy Swiss-Prot
Match: Q8H1S4 (1-aminocyclopropane-1-carboxylate oxidase homolog 3 OS=Arabidopsis thaliana OX=3702 GN=At1g06650 PE=2 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 3.2e-79
Identity = 142/279 (50.90%), Postives = 193/279 (69.18%), Query Frame = 0


Query: 9   ESLSSRRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNA--AAESEL----SIPI 68
           + L  R +EL++FD +K G KGL D+G++++P IFH     L+      S+L    +IP 
Sbjct: 7   DPLFDRASELKAFDETKTGVKGLVDSGVSQVPRIFHHPTVKLSTPKPLPSDLLHLKTIPT 66

Query: 69  IDLHGGE-------EVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEW 128
           IDL G +          +++I  A  KWGFFQV+NHG+ +E+L+ M  G+R FHEQ  E 
Sbjct: 67  IDLGGRDFQDAIKRNNAIEEIKEAAAKWGFFQVINHGVSLELLEKMKKGVRDFHEQSQEV 126

Query: 129 KKQFYSRDRGKKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTK 188
           +K+FYSRD  ++  Y SNFDLF S AANW+DT S +MAP  P P ++PEICR+I++EY+K
Sbjct: 127 RKEFYSRDFSRRFLYLSNFDLFSSPAANWRDTFSCTMAPDTPKPQDLPEICRDIMMEYSK 186

Query: 189 EVKKLGWILLELISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAF 248
           +V  LG  L EL+S+ALGLE +HL D+ C+ G+LMLSHYYPPCPEP LT+GT+ H+D +F
Sbjct: 187 QVMNLGKFLFELLSEALGLEPNHLNDMDCSKGLLMLSHYYPPCPEPDLTLGTSQHSDNSF 246

Query: 249 ITILLQDQIGGLQVLHHNRWLHVPPLHGALVINLGDFLQ 275
           +T+LL DQI GLQV     W  VP + GAL+IN+GD LQ
Sbjct: 247 LTVLLPDQIEGLQVRREGHWFDVPHVSGALIINIGDLLQ 285

[Close]

BLAST of MC07g_new0370 vs. ExPASy Swiss-Prot
Match: Q94A78 (1-aminocyclopropane-1-carboxylate oxidase homolog 4 OS=Arabidopsis thaliana OX=3702 GN=At1g03400 PE=2 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 4.6e-78
Identity = 139/267 (52.06%), Postives = 183/267 (68.54%), Query Frame = 0


Query: 12  SSRRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDL----NAAAESELSIPIIDLHG 71
           + R ++ ++FD +K G KGL D+GIT+IP +F      L    +      L+IP +DL G
Sbjct: 4   TDRSSQAKAFDEAKIGVKGLVDSGITEIPALFRATPATLASLKSPPPPKHLTIPTVDLKG 63

Query: 72  GEEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDRGKK 131
               V+++IG A  KWG F +VNHGIPVEVL+ MI GIRGFHEQ+ E KK+FYSRD  + 
Sbjct: 64  AS--VVEKIGEAAEKWGLFHLVNHGIPVEVLERMIQGIRGFHEQEPEAKKRFYSRDHTRD 123

Query: 132 VAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWILLEL 191
           V YFSN DL  S+AA+W+DTL    AP  P   ++P +C EI++EY+KE+  LG  L EL
Sbjct: 124 VLYFSNHDLQNSEAASWRDTLGCYTAPEPPRLEDLPAVCGEIMLEYSKEIMSLGERLFEL 183

Query: 192 ISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQIGGL 251
           +S+ALGL   HLKD+ CA    M+  +YPPCP+P LTIG   HTD++F+T+LLQD +GGL
Sbjct: 184 LSEALGLNSHHLKDMDCAKSQYMVGQHYPPCPQPDLTIGINKHTDISFLTVLLQDNVGGL 243

Query: 252 QVLHHNRWLHVPPLHGALVINLGDFLQ 275
           QV H   W+ V P+ GALVIN+GDFLQ
Sbjct: 244 QVFHEQYWIDVTPVPGALVINIGDFLQ 268

[Close]

BLAST of MC07g_new0370 vs. ExPASy Swiss-Prot
Match: Q43383 (1-aminocyclopropane-1-carboxylate oxidase homolog 5 OS=Arabidopsis thaliana OX=3702 GN=2A6 PE=2 SV=2)

HSP 1 Score: 292.4 bits (747), Expect = 6.0e-78
Identity = 143/275 (52.00%), Postives = 188/275 (68.36%), Query Frame = 0


Query: 12  SSRRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDL-----NAAAESELSIPIIDLH 71
           S R ++ ++FD +K G KGL  +GI +IP +FH     L      A    +L+IP +DL 
Sbjct: 41  SDRSSQAKAFDETKTGVKGLVASGIKEIPAMFHTPPDTLTSLKQTAPPSQQLTIPTVDLK 100

Query: 72  GG------EEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFY 131
           GG         V+++IG A  +WGFFQVVNHGI VEV++ M +GIR FHEQD E KK+FY
Sbjct: 101 GGSMDLISRRSVVEKIGDAAERWGFFQVVNHGISVEVMERMKEGIRRFHEQDPEVKKRFY 160

Query: 132 SRDRGKKVAYFSNFDLFQ-SKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKK 191
           SRD  + V Y+SN DL   +KAANW+DTL+  MAP  P   ++P +C EI++EY+K++  
Sbjct: 161 SRDHTRDVLYYSNIDLHTCNKAANWRDTLACYMAPDPPKLQDLPAVCGEIMMEYSKQLMT 220

Query: 192 LGWILLELISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITIL 251
           LG  L EL+S+ALGL  +HLKD+GCA   +M   YYPPCP+P LT+G + HTD +FITIL
Sbjct: 221 LGEFLFELLSEALGLNPNHLKDMGCAKSHIMFGQYYPPCPQPDLTLGISKHTDFSFITIL 280

Query: 252 LQDQIGGLQVLHHNRWLHVPPLHGALVINLGDFLQ 275
           LQD IGGLQV+H   W+ V P+ GALVIN+GD LQ
Sbjct: 281 LQDNIGGLQVIHDQCWVDVSPVPGALVINIGDLLQ 315

[Close]

BLAST of MC07g_new0370 vs. ExPASy Swiss-Prot
Match: O04847 (Deacetoxyvindoline 4-hydroxylase OS=Catharanthus roseus OX=4058 GN=D4H PE=1 SV=2)

HSP 1 Score: 291.6 bits (745), Expect = 1.0e-77
Identity = 136/269 (50.56%), Postives = 195/269 (72.49%), Query Frame = 0


Query: 17  ELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLN----AAAESELSIPIIDLHG---GE 76
           EL++FD +KAG KG+ D GITKIP IF  Q ++L+       +S++ IP+I+L+G     
Sbjct: 44  ELKAFDETKAGVKGIVDTGITKIPRIFIDQPKNLDRISVCRGKSDIKIPVINLNGLSSNS 103

Query: 77  EV---VMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDR-G 136
           E+   ++++IG A  K+GFFQ+VNHGIP +V+D M+DG+R FHEQD + K+Q+YSRDR  
Sbjct: 104 EIRREIVEKIGEASEKYGFFQIVNHGIPQDVMDKMVDGVRKFHEQDDQIKRQYYSRDRFN 163

Query: 137 KKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWILL 196
           K   Y SN+ L    A NW+DT+   M    PDP E P++CR+I+++Y+  V+ LG IL 
Sbjct: 164 KNFLYSSNYVLIPGIACNWRDTMECIMNSNQPDPQEFPDVCRDILMKYSNYVRNLGLILF 223

Query: 197 ELISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQIG 256
           EL+S+ALGL+ +HL+++ CA+G+++L HYYP CP+P LT GT+ H+D  F+TIL+QDQIG
Sbjct: 224 ELLSEALGLKPNHLEEMDCAEGLILLGHYYPACPQPELTFGTSKHSDSGFLTILMQDQIG 283

Query: 257 GLQVLHHNRWLHVPPLHGALVINLGDFLQ 275
           GLQ+L  N+W+ VP + GALVIN+ D LQ
Sbjct: 284 GLQILLENQWIDVPFIPGALVINIADLLQ 312

[Close]

BLAST of MC07g_new0370 vs. NCBI nr
Match: XP_022138649.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like [Momordica charantia])

HSP 1 Score: 570 bits (1469), Expect = 1.41e-203
Identity = 274/274 (100.00%), Postives = 274/274 (100.00%), Query Frame = 0


Query: 1   MAAIGTGTESLSSRRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNAAAESELSI 60
           MAAIGTGTESLSSRRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNAAAESELSI
Sbjct: 1   MAAIGTGTESLSSRRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNAAAESELSI 60

Query: 61  PIIDLHGGEEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFY 120
           PIIDLHGGEEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFY
Sbjct: 61  PIIDLHGGEEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFY 120

Query: 121 SRDRGKKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKL 180
           SRDRGKKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKL
Sbjct: 121 SRDRGKKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKL 180

Query: 181 GWILLELISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILL 240
           GWILLELISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILL
Sbjct: 181 GWILLELISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILL 240

Query: 241 QDQIGGLQVLHHNRWLHVPPLHGALVINLGDFLQ 274
           QDQIGGLQVLHHNRWLHVPPLHGALVINLGDFLQ
Sbjct: 241 QDQIGGLQVLHHNRWLHVPPLHGALVINLGDFLQ 274

[Close]

BLAST of MC07g_new0370 vs. NCBI nr
Match: XP_022930403.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X4 [Cucurbita moschata])

HSP 1 Score: 420 bits (1080), Expect = 2.34e-144
Identity = 206/277 (74.37%), Postives = 234/277 (84.48%), Query Frame = 0


Query: 1   MAAIGTGTESLSSRRAELESFDNSKAGAKGLSDAGITKIPPIFHC--QKQDLNAAAESEL 60
           MAAI T  ESL+SRR++LE+F+N+K+G KGL DAGITKIP IFHC  +     AA ESE 
Sbjct: 1   MAAIKT--ESLNSRRSQLEAFENTKSGVKGLLDAGITKIPSIFHCHVKPHTKTAATESES 60

Query: 61  SIPIIDLHGGEEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQ 120
           SIPIIDLHG  E V+D+I +ACRKWGFFQVVNHGIPVEVL+ +I+G+RGFHEQD E KKQ
Sbjct: 61  SIPIIDLHGSVEEVVDEIRNACRKWGFFQVVNHGIPVEVLEQVINGVRGFHEQDPELKKQ 120

Query: 121 FYSRDRGKKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVK 180
           FYSRDR KKV+YFSNFDLFQSKAANW+DTLS SMAP  P P EIP+ICREIV+EYT++VK
Sbjct: 121 FYSRDRTKKVSYFSNFDLFQSKAANWRDTLSFSMAPSLPLPEEIPQICREIVMEYTEQVK 180

Query: 181 KLGWILLELISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITI 240
           KLG  L ELISKAL L  +HLKD+GCA+G+LML HYYP CPEP LT+GT NHTDVAFITI
Sbjct: 181 KLGLTLFELISKALELGDTHLKDMGCAEGLLMLGHYYPACPEPDLTLGTTNHTDVAFITI 240

Query: 241 LLQDQIGGLQVLHHNRWLHVPPLHGALVINLGDFLQE 275
           LLQDQIGGLQV H N+W  VPPLHGALVIN GDFLQ+
Sbjct: 241 LLQDQIGGLQVFHDNQWFDVPPLHGALVINAGDFLQQ 275

[Close]

BLAST of MC07g_new0370 vs. NCBI nr
Match: XP_022930402.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 420 bits (1080), Expect = 4.67e-144
Identity = 206/277 (74.37%), Postives = 234/277 (84.48%), Query Frame = 0


Query: 1   MAAIGTGTESLSSRRAELESFDNSKAGAKGLSDAGITKIPPIFHC--QKQDLNAAAESEL 60
           MAAI T  ESL+SRR++LE+F+N+K+G KGL DAGITKIP IFHC  +     AA ESE 
Sbjct: 21  MAAIKT--ESLNSRRSQLEAFENTKSGVKGLLDAGITKIPSIFHCHVKPHTKTAATESES 80

Query: 61  SIPIIDLHGGEEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQ 120
           SIPIIDLHG  E V+D+I +ACRKWGFFQVVNHGIPVEVL+ +I+G+RGFHEQD E KKQ
Sbjct: 81  SIPIIDLHGSVEEVVDEIRNACRKWGFFQVVNHGIPVEVLEQVINGVRGFHEQDPELKKQ 140

Query: 121 FYSRDRGKKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVK 180
           FYSRDR KKV+YFSNFDLFQSKAANW+DTLS SMAP  P P EIP+ICREIV+EYT++VK
Sbjct: 141 FYSRDRTKKVSYFSNFDLFQSKAANWRDTLSFSMAPSLPLPEEIPQICREIVMEYTEQVK 200

Query: 181 KLGWILLELISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITI 240
           KLG  L ELISKAL L  +HLKD+GCA+G+LML HYYP CPEP LT+GT NHTDVAFITI
Sbjct: 201 KLGLTLFELISKALELGDTHLKDMGCAEGLLMLGHYYPACPEPDLTLGTTNHTDVAFITI 260

Query: 241 LLQDQIGGLQVLHHNRWLHVPPLHGALVINLGDFLQE 275
           LLQDQIGGLQV H N+W  VPPLHGALVIN GDFLQ+
Sbjct: 261 LLQDQIGGLQVFHDNQWFDVPPLHGALVINAGDFLQQ 295

[Close]

BLAST of MC07g_new0370 vs. NCBI nr
Match: XP_022930400.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 420 bits (1080), Expect = 1.35e-143
Identity = 206/277 (74.37%), Postives = 234/277 (84.48%), Query Frame = 0


Query: 1   MAAIGTGTESLSSRRAELESFDNSKAGAKGLSDAGITKIPPIFHC--QKQDLNAAAESEL 60
           MAAI T  ESL+SRR++LE+F+N+K+G KGL DAGITKIP IFHC  +     AA ESE 
Sbjct: 52  MAAIKT--ESLNSRRSQLEAFENTKSGVKGLLDAGITKIPSIFHCHVKPHTKTAATESES 111

Query: 61  SIPIIDLHGGEEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQ 120
           SIPIIDLHG  E V+D+I +ACRKWGFFQVVNHGIPVEVL+ +I+G+RGFHEQD E KKQ
Sbjct: 112 SIPIIDLHGSVEEVVDEIRNACRKWGFFQVVNHGIPVEVLEQVINGVRGFHEQDPELKKQ 171

Query: 121 FYSRDRGKKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVK 180
           FYSRDR KKV+YFSNFDLFQSKAANW+DTLS SMAP  P P EIP+ICREIV+EYT++VK
Sbjct: 172 FYSRDRTKKVSYFSNFDLFQSKAANWRDTLSFSMAPSLPLPEEIPQICREIVMEYTEQVK 231

Query: 181 KLGWILLELISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITI 240
           KLG  L ELISKAL L  +HLKD+GCA+G+LML HYYP CPEP LT+GT NHTDVAFITI
Sbjct: 232 KLGLTLFELISKALELGDTHLKDMGCAEGLLMLGHYYPACPEPDLTLGTTNHTDVAFITI 291

Query: 241 LLQDQIGGLQVLHHNRWLHVPPLHGALVINLGDFLQE 275
           LLQDQIGGLQV H N+W  VPPLHGALVIN GDFLQ+
Sbjct: 292 LLQDQIGGLQVFHDNQWFDVPPLHGALVINAGDFLQQ 326

[Close]

BLAST of MC07g_new0370 vs. NCBI nr
Match: XP_022930401.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 419 bits (1077), Expect = 3.72e-143
Identity = 206/276 (74.64%), Postives = 233/276 (84.42%), Query Frame = 0


Query: 1   MAAIGTGTESLSSRRAELESFDNSKAGAKGLSDAGITKIPPIFHC--QKQDLNAAAESEL 60
           MAAI T  ESL+SRR++LE+F+N+K+G KGL DAGITKIP IFHC  +     AA ESE 
Sbjct: 52  MAAIKT--ESLNSRRSQLEAFENTKSGVKGLLDAGITKIPSIFHCHVKPHTKTAATESES 111

Query: 61  SIPIIDLHGGEEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQ 120
           SIPIIDLHG  E V+D+I +ACRKWGFFQVVNHGIPVEVL+ +I+G+RGFHEQD E KKQ
Sbjct: 112 SIPIIDLHGSVEEVVDEIRNACRKWGFFQVVNHGIPVEVLEQVINGVRGFHEQDPELKKQ 171

Query: 121 FYSRDRGKKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVK 180
           FYSRDR KKV+YFSNFDLFQSKAANW+DTLS SMAP  P P EIP+ICREIV+EYT++VK
Sbjct: 172 FYSRDRTKKVSYFSNFDLFQSKAANWRDTLSFSMAPSLPLPEEIPQICREIVMEYTEQVK 231

Query: 181 KLGWILLELISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITI 240
           KLG  L ELISKAL L  +HLKD+GCA+G+LML HYYP CPEP LT+GT NHTDVAFITI
Sbjct: 232 KLGLTLFELISKALELGDTHLKDMGCAEGLLMLGHYYPACPEPDLTLGTTNHTDVAFITI 291

Query: 241 LLQDQIGGLQVLHHNRWLHVPPLHGALVINLGDFLQ 274
           LLQDQIGGLQV H N+W  VPPLHGALVIN GDFLQ
Sbjct: 292 LLQDQIGGLQVFHDNQWFDVPPLHGALVINAGDFLQ 325

[Close]

BLAST of MC07g_new0370 vs. ExPASy TrEMBL
Match: A0A6J1CAP5 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like OS=Momordica charantia OX=3673 GN=LOC111009755 PE=3 SV=1)

HSP 1 Score: 570 bits (1469), Expect = 6.85e-204
Identity = 274/274 (100.00%), Postives = 274/274 (100.00%), Query Frame = 0


Query: 1   MAAIGTGTESLSSRRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNAAAESELSI 60
           MAAIGTGTESLSSRRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNAAAESELSI
Sbjct: 1   MAAIGTGTESLSSRRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNAAAESELSI 60

Query: 61  PIIDLHGGEEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFY 120
           PIIDLHGGEEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFY
Sbjct: 61  PIIDLHGGEEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFY 120

Query: 121 SRDRGKKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKL 180
           SRDRGKKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKL
Sbjct: 121 SRDRGKKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKL 180

Query: 181 GWILLELISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILL 240
           GWILLELISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILL
Sbjct: 181 GWILLELISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILL 240

Query: 241 QDQIGGLQVLHHNRWLHVPPLHGALVINLGDFLQ 274
           QDQIGGLQVLHHNRWLHVPPLHGALVINLGDFLQ
Sbjct: 241 QDQIGGLQVLHHNRWLHVPPLHGALVINLGDFLQ 274

[Close]

BLAST of MC07g_new0370 vs. ExPASy TrEMBL
Match: A0A6J1EV24 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111436859 PE=3 SV=1)

HSP 1 Score: 420 bits (1080), Expect = 1.13e-144
Identity = 206/277 (74.37%), Postives = 234/277 (84.48%), Query Frame = 0


Query: 1   MAAIGTGTESLSSRRAELESFDNSKAGAKGLSDAGITKIPPIFHC--QKQDLNAAAESEL 60
           MAAI T  ESL+SRR++LE+F+N+K+G KGL DAGITKIP IFHC  +     AA ESE 
Sbjct: 1   MAAIKT--ESLNSRRSQLEAFENTKSGVKGLLDAGITKIPSIFHCHVKPHTKTAATESES 60

Query: 61  SIPIIDLHGGEEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQ 120
           SIPIIDLHG  E V+D+I +ACRKWGFFQVVNHGIPVEVL+ +I+G+RGFHEQD E KKQ
Sbjct: 61  SIPIIDLHGSVEEVVDEIRNACRKWGFFQVVNHGIPVEVLEQVINGVRGFHEQDPELKKQ 120

Query: 121 FYSRDRGKKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVK 180
           FYSRDR KKV+YFSNFDLFQSKAANW+DTLS SMAP  P P EIP+ICREIV+EYT++VK
Sbjct: 121 FYSRDRTKKVSYFSNFDLFQSKAANWRDTLSFSMAPSLPLPEEIPQICREIVMEYTEQVK 180

Query: 181 KLGWILLELISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITI 240
           KLG  L ELISKAL L  +HLKD+GCA+G+LML HYYP CPEP LT+GT NHTDVAFITI
Sbjct: 181 KLGLTLFELISKALELGDTHLKDMGCAEGLLMLGHYYPACPEPDLTLGTTNHTDVAFITI 240

Query: 241 LLQDQIGGLQVLHHNRWLHVPPLHGALVINLGDFLQE 275
           LLQDQIGGLQV H N+W  VPPLHGALVIN GDFLQ+
Sbjct: 241 LLQDQIGGLQVFHDNQWFDVPPLHGALVINAGDFLQQ 275

[Close]

BLAST of MC07g_new0370 vs. ExPASy TrEMBL
Match: A0A6J1EQD8 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111436859 PE=3 SV=1)

HSP 1 Score: 420 bits (1080), Expect = 2.26e-144
Identity = 206/277 (74.37%), Postives = 234/277 (84.48%), Query Frame = 0


Query: 1   MAAIGTGTESLSSRRAELESFDNSKAGAKGLSDAGITKIPPIFHC--QKQDLNAAAESEL 60
           MAAI T  ESL+SRR++LE+F+N+K+G KGL DAGITKIP IFHC  +     AA ESE 
Sbjct: 21  MAAIKT--ESLNSRRSQLEAFENTKSGVKGLLDAGITKIPSIFHCHVKPHTKTAATESES 80

Query: 61  SIPIIDLHGGEEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQ 120
           SIPIIDLHG  E V+D+I +ACRKWGFFQVVNHGIPVEVL+ +I+G+RGFHEQD E KKQ
Sbjct: 81  SIPIIDLHGSVEEVVDEIRNACRKWGFFQVVNHGIPVEVLEQVINGVRGFHEQDPELKKQ 140

Query: 121 FYSRDRGKKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVK 180
           FYSRDR KKV+YFSNFDLFQSKAANW+DTLS SMAP  P P EIP+ICREIV+EYT++VK
Sbjct: 141 FYSRDRTKKVSYFSNFDLFQSKAANWRDTLSFSMAPSLPLPEEIPQICREIVMEYTEQVK 200

Query: 181 KLGWILLELISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITI 240
           KLG  L ELISKAL L  +HLKD+GCA+G+LML HYYP CPEP LT+GT NHTDVAFITI
Sbjct: 201 KLGLTLFELISKALELGDTHLKDMGCAEGLLMLGHYYPACPEPDLTLGTTNHTDVAFITI 260

Query: 241 LLQDQIGGLQVLHHNRWLHVPPLHGALVINLGDFLQE 275
           LLQDQIGGLQV H N+W  VPPLHGALVIN GDFLQ+
Sbjct: 261 LLQDQIGGLQVFHDNQWFDVPPLHGALVINAGDFLQQ 295

[Close]

BLAST of MC07g_new0370 vs. ExPASy TrEMBL
Match: A0A6J1EWT9 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436859 PE=3 SV=1)

HSP 1 Score: 420 bits (1080), Expect = 6.52e-144
Identity = 206/277 (74.37%), Postives = 234/277 (84.48%), Query Frame = 0


Query: 1   MAAIGTGTESLSSRRAELESFDNSKAGAKGLSDAGITKIPPIFHC--QKQDLNAAAESEL 60
           MAAI T  ESL+SRR++LE+F+N+K+G KGL DAGITKIP IFHC  +     AA ESE 
Sbjct: 52  MAAIKT--ESLNSRRSQLEAFENTKSGVKGLLDAGITKIPSIFHCHVKPHTKTAATESES 111

Query: 61  SIPIIDLHGGEEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQ 120
           SIPIIDLHG  E V+D+I +ACRKWGFFQVVNHGIPVEVL+ +I+G+RGFHEQD E KKQ
Sbjct: 112 SIPIIDLHGSVEEVVDEIRNACRKWGFFQVVNHGIPVEVLEQVINGVRGFHEQDPELKKQ 171

Query: 121 FYSRDRGKKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVK 180
           FYSRDR KKV+YFSNFDLFQSKAANW+DTLS SMAP  P P EIP+ICREIV+EYT++VK
Sbjct: 172 FYSRDRTKKVSYFSNFDLFQSKAANWRDTLSFSMAPSLPLPEEIPQICREIVMEYTEQVK 231

Query: 181 KLGWILLELISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITI 240
           KLG  L ELISKAL L  +HLKD+GCA+G+LML HYYP CPEP LT+GT NHTDVAFITI
Sbjct: 232 KLGLTLFELISKALELGDTHLKDMGCAEGLLMLGHYYPACPEPDLTLGTTNHTDVAFITI 291

Query: 241 LLQDQIGGLQVLHHNRWLHVPPLHGALVINLGDFLQE 275
           LLQDQIGGLQV H N+W  VPPLHGALVIN GDFLQ+
Sbjct: 292 LLQDQIGGLQVFHDNQWFDVPPLHGALVINAGDFLQQ 326

[Close]

BLAST of MC07g_new0370 vs. ExPASy TrEMBL
Match: A0A6J1EQU3 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436859 PE=3 SV=1)

HSP 1 Score: 419 bits (1077), Expect = 1.80e-143
Identity = 206/276 (74.64%), Postives = 233/276 (84.42%), Query Frame = 0


Query: 1   MAAIGTGTESLSSRRAELESFDNSKAGAKGLSDAGITKIPPIFHC--QKQDLNAAAESEL 60
           MAAI T  ESL+SRR++LE+F+N+K+G KGL DAGITKIP IFHC  +     AA ESE 
Sbjct: 52  MAAIKT--ESLNSRRSQLEAFENTKSGVKGLLDAGITKIPSIFHCHVKPHTKTAATESES 111

Query: 61  SIPIIDLHGGEEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQ 120
           SIPIIDLHG  E V+D+I +ACRKWGFFQVVNHGIPVEVL+ +I+G+RGFHEQD E KKQ
Sbjct: 112 SIPIIDLHGSVEEVVDEIRNACRKWGFFQVVNHGIPVEVLEQVINGVRGFHEQDPELKKQ 171

Query: 121 FYSRDRGKKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVK 180
           FYSRDR KKV+YFSNFDLFQSKAANW+DTLS SMAP  P P EIP+ICREIV+EYT++VK
Sbjct: 172 FYSRDRTKKVSYFSNFDLFQSKAANWRDTLSFSMAPSLPLPEEIPQICREIVMEYTEQVK 231

Query: 181 KLGWILLELISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITI 240
           KLG  L ELISKAL L  +HLKD+GCA+G+LML HYYP CPEP LT+GT NHTDVAFITI
Sbjct: 232 KLGLTLFELISKALELGDTHLKDMGCAEGLLMLGHYYPACPEPDLTLGTTNHTDVAFITI 291

Query: 241 LLQDQIGGLQVLHHNRWLHVPPLHGALVINLGDFLQ 274
           LLQDQIGGLQV H N+W  VPPLHGALVIN GDFLQ
Sbjct: 292 LLQDQIGGLQVFHDNQWFDVPPLHGALVINAGDFLQ 325

[Close]

BLAST of MC07g_new0370 vs. TAIR 10
Match: AT1G06620.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 317.4 bits (812), Expect = 1.2e-86
Identity = 152/270 (56.30%), Postives = 191/270 (70.74%), Query Frame = 0


Query: 14  RRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNA---AAESELSIPIIDLHGG-- 73
           R   L++FD +K G KGL DAGIT+IP IF      L +    + S+ SIP IDL GG  
Sbjct: 13  RSTLLKAFDETKTGVKGLIDAGITEIPSIFRAPPATLTSPKPPSSSDFSIPTIDLKGGGT 72

Query: 74  ----EEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDR 133
                  ++++IG A  KWGFFQV+NHGIP++VL+ MIDGIR FHEQD E KK FYSRD 
Sbjct: 73  DSITRRSLVEKIGDAAEKWGFFQVINHGIPMDVLEKMIDGIREFHEQDTEVKKGFYSRDP 132

Query: 134 GKKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWIL 193
             K+ Y SNFDLF S AANW+DTL    AP  P P ++P  C E++IEY+KEV KLG +L
Sbjct: 133 ASKMVYSSNFDLFSSPAANWRDTLGCYTAPDPPRPEDLPATCGEMMIEYSKEVMKLGKLL 192

Query: 194 LELISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQI 253
            EL+S+ALGL  +HLKD+ C + +L+L HYYPPCP+P LT+G   H+D +F+TILLQD I
Sbjct: 193 FELLSEALGLNTNHLKDMDCTNSLLLLGHYYPPCPQPDLTLGLTKHSDNSFLTILLQDHI 252

Query: 254 GGLQVLHHNRWLHVPPLHGALVINLGDFLQ 275
           GGLQVLH   W+ VPP+ GALV+N+GD LQ
Sbjct: 253 GGLQVLHDQYWVDVPPVPGALVVNVGDLLQ 282

[Close]

BLAST of MC07g_new0370 vs. TAIR 10
Match: AT2G30830.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 314.3 bits (804), Expect = 1.0e-85
Identity = 151/269 (56.13%), Postives = 196/269 (72.86%), Query Frame = 0


Query: 14  RRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDL-NAAAESELSIPIIDLHGG---- 73
           R  E+++FD  K G KGL DAGITK+P IFH Q   + N    S L IP ID+ GG    
Sbjct: 7   RAGEVKAFDQMKIGVKGLVDAGITKVPRIFHHQDVAVTNPKPSSTLEIPTIDVGGGVFES 66

Query: 74  ---EEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDRG 133
               + V+ ++ +A  K+GFFQV+NHGIP+EV++ M DGIRGFHEQD E KK FYSRD  
Sbjct: 67  TVTRKSVIAKVRAAVEKFGFFQVINHGIPLEVMESMKDGIRGFHEQDSEVKKTFYSRDIT 126

Query: 134 KKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWILL 193
           KKV Y +NFDL+ S+AANW+DTL++ MAP  P   ++P ICREI++EY+K + KLG ++ 
Sbjct: 127 KKVKYNTNFDLYSSQAANWRDTLTMVMAPDVPQAGDLPVICREIMLEYSKRMMKLGELIF 186

Query: 194 ELISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQIG 253
           EL+S+ALGL+ +HLK++ CA  + +LSHYYPPCPEP  T G ++HTD++FITILLQD IG
Sbjct: 187 ELLSEALGLKPNHLKELNCAKSLSLLSHYYPPCPEPDRTFGISSHTDISFITILLQDHIG 246

Query: 254 GLQVLHHNRWLHVPPLHGALVINLGDFLQ 275
           GLQVLH   W+ VPP   AL++NLGD LQ
Sbjct: 247 GLQVLHDGYWIDVPPNPEALIVNLGDLLQ 275

[Close]

BLAST of MC07g_new0370 vs. TAIR 10
Match: AT2G30840.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 298.5 bits (763), Expect = 5.9e-81
Identity = 146/278 (52.52%), Postives = 195/278 (70.14%), Query Frame = 0


Query: 9   ESLSSRRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNAA----AESELSIPIID 68
           E+   R +E+++FD  K G KGL DAG+T+IP IFH    +L  +    + + + IP ID
Sbjct: 2   EATYDRASEVKAFDELKIGVKGLLDAGVTQIPRIFHHPHLNLTDSNLLLSSTTMVIPTID 61

Query: 69  LHGG--------EEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWK 128
           L GG         E V+  I  A  ++GFFQV+NHGI  +V++ M DGIRGFHEQD + +
Sbjct: 62  LKGGVFDEYTVTRESVIAMIRDAVERFGFFQVINHGISNDVMEKMKDGIRGFHEQDSDVR 121

Query: 129 KQFYSRDRGKKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKE 188
           K+FY+RD  K V Y SNFDL+ S +ANW+DTLS  MAP  P+  ++P+IC EI++EY K 
Sbjct: 122 KKFYTRDVTKTVKYNSNFDLYSSPSANWRDTLSCFMAPDVPETEDLPDICGEIMLEYAKR 181

Query: 189 VKKLGWILLELISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFI 248
           V KLG ++ EL+S+ALGL  +HLK++ C  G+LMLSHYYPPCPEP LT GT+ H+D +F+
Sbjct: 182 VMKLGELIFELLSEALGLNPNHLKEMDCTKGLLMLSHYYPPCPEPGLTFGTSPHSDRSFL 241

Query: 249 TILLQDQIGGLQVLHHNRWLHVPPLHGALVINLGDFLQ 275
           TILLQD IGGLQV  +  W+ VPP+ GAL++NLGD LQ
Sbjct: 242 TILLQDHIGGLQVRQNGYWVDVPPVPGALLVNLGDLLQ 279

[Close]

BLAST of MC07g_new0370 vs. TAIR 10
Match: AT1G06650.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 297.4 bits (760), Expect = 1.3e-80
Identity = 143/282 (50.71%), Postives = 194/282 (68.79%), Query Frame = 0


Query: 9   ESLSSRRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNA--AAESEL----SIPI 68
           + L  R +EL++FD +K G KGL D+G++++P IFH     L+      S+L    +IP 
Sbjct: 7   DPLFDRASELKAFDETKTGVKGLVDSGVSQVPRIFHHPTVKLSTPKPLPSDLLHLKTIPT 66

Query: 69  IDLHGGE-------EVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEW 128
           IDL G +          +++I  A  KWGFFQV+NHG+ +E+L+ M  G+R FHEQ  E 
Sbjct: 67  IDLGGRDFQDAIKRNNAIEEIKEAAAKWGFFQVINHGVSLELLEKMKKGVRDFHEQSQEV 126

Query: 129 KKQFYSRDRGKKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTK 188
           +K+FYSRD  ++  Y SNFDLF S AANW+DT S +MAP  P P ++PEICR+I++EY+K
Sbjct: 127 RKEFYSRDFSRRFLYLSNFDLFSSPAANWRDTFSCTMAPDTPKPQDLPEICRDIMMEYSK 186

Query: 189 EVKKLGWILLELISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAF 248
           +V  LG  L EL+S+ALGLE +HL D+ C+ G+LMLSHYYPPCPEP LT+GT+ H+D +F
Sbjct: 187 QVMNLGKFLFELLSEALGLEPNHLNDMDCSKGLLMLSHYYPPCPEPDLTLGTSQHSDNSF 246

Query: 249 ITILLQDQIGGLQVLHHNRWLHVPPLHGALVINLGDFLQESA 278
           +T+LL DQI GLQV     W  VP + GAL+IN+GD LQ  A
Sbjct: 247 LTVLLPDQIEGLQVRREGHWFDVPHVSGALIINIGDLLQFGA 288

[Close]

BLAST of MC07g_new0370 vs. TAIR 10
Match: AT1G06650.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 296.6 bits (758), Expect = 2.3e-80
Identity = 142/279 (50.90%), Postives = 193/279 (69.18%), Query Frame = 0


Query: 9   ESLSSRRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNA--AAESEL----SIPI 68
           + L  R +EL++FD +K G KGL D+G++++P IFH     L+      S+L    +IP 
Sbjct: 7   DPLFDRASELKAFDETKTGVKGLVDSGVSQVPRIFHHPTVKLSTPKPLPSDLLHLKTIPT 66

Query: 69  IDLHGGE-------EVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEW 128
           IDL G +          +++I  A  KWGFFQV+NHG+ +E+L+ M  G+R FHEQ  E 
Sbjct: 67  IDLGGRDFQDAIKRNNAIEEIKEAAAKWGFFQVINHGVSLELLEKMKKGVRDFHEQSQEV 126

Query: 129 KKQFYSRDRGKKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTK 188
           +K+FYSRD  ++  Y SNFDLF S AANW+DT S +MAP  P P ++PEICR+I++EY+K
Sbjct: 127 RKEFYSRDFSRRFLYLSNFDLFSSPAANWRDTFSCTMAPDTPKPQDLPEICRDIMMEYSK 186

Query: 189 EVKKLGWILLELISKALGLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAF 248
           +V  LG  L EL+S+ALGLE +HL D+ C+ G+LMLSHYYPPCPEP LT+GT+ H+D +F
Sbjct: 187 QVMNLGKFLFELLSEALGLEPNHLNDMDCSKGLLMLSHYYPPCPEPDLTLGTSQHSDNSF 246

Query: 249 ITILLQDQIGGLQVLHHNRWLHVPPLHGALVINLGDFLQ 275
           +T+LL DQI GLQV     W  VP + GAL+IN+GD LQ
Sbjct: 247 LTVLLPDQIEGLQVRREGHWFDVPHVSGALIINIGDLLQ 285

The following BLAST results are available for this feature:

BLAST of MC07g_new0370 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84MB3	1.7e-85	56.30	1-aminocyclopropane-1-carboxylate oxidase homolog 1 OS=Arabidopsis thaliana OX=3...	[more]
Q8H1S4	3.2e-79	50.90	1-aminocyclopropane-1-carboxylate oxidase homolog 3 OS=Arabidopsis thaliana OX=3...	[more]
Q94A78	4.6e-78	52.06	1-aminocyclopropane-1-carboxylate oxidase homolog 4 OS=Arabidopsis thaliana OX=3...	[more]
Q43383	6.0e-78	52.00	1-aminocyclopropane-1-carboxylate oxidase homolog 5 OS=Arabidopsis thaliana OX=3...	[more]
O04847	1.0e-77	50.56	Deacetoxyvindoline 4-hydroxylase OS=Catharanthus roseus OX=4058 GN=D4H PE=1 SV=2	[more]

BLAST of MC07g_new0370 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022138649.1	1.41e-203	100.00	1-aminocyclopropane-1-carboxylate oxidase homolog 1-like [Momordica charantia]	[more]
XP_022930403.1	2.34e-144	74.37	1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X4 [Cucurbita m...	[more]
XP_022930402.1	4.67e-144	74.37	1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X3 [Cucurbita m...	[more]
XP_022930400.1	1.35e-143	74.37	1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X1 [Cucurbita m...	[more]
XP_022930401.1	3.72e-143	74.64	1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X2 [Cucurbita m...	[more]

BLAST of MC07g_new0370 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CAP5	6.85e-204	100.00	1-aminocyclopropane-1-carboxylate oxidase homolog 1-like OS=Momordica charantia ...	[more]
A0A6J1EV24	1.13e-144	74.37	1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X4 OS=Cucurbita...	[more]
A0A6J1EQD8	2.26e-144	74.37	1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X3 OS=Cucurbita...	[more]
A0A6J1EWT9	6.52e-144	74.37	1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X1 OS=Cucurbita...	[more]
A0A6J1EQU3	1.80e-143	74.64	1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X2 OS=Cucurbita...	[more]

BLAST of MC07g_new0370 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G06620.1	1.2e-86	56.30	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G30830.1	1.0e-85	56.13	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G30840.1	5.9e-81	52.52	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G06650.1	1.3e-80	50.71	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G06650.2	2.3e-80	50.90	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 210..274 e-value: 1.1E-15 score: 57.9
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 60..154 e-value: 1.1E-17 score: 64.9
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 19..285 e-value: 1.7E-77 score: 263.1
None	No IPR available	PANTHER	PTHR10209:SF721	1-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE HOMOLOG 1-LIKE	coord: 9..280
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 9..280
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 26..274
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 204..298 score: 8.478112

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC07g_new0370.1	MC07g_new0370.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016491	oxidoreductase activity