MC07g_new0035 (gene) Bitter gourd (Dali-11) v1

BLAST of MC07g_new0035 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.1e-10
Identity = 53/177 (29.94%), Postives = 80/177 (45.20%), Query Frame = 0

Query: 54  NENSPFKAEGRVSCQICGKFNHTAIEC--WNRFDHSFQLEDLPKALAAFNLKDT------ 113
           N++ P+  +    CQICG   H+A  C     F  S   +  P     +  +        
Sbjct: 270 NQSKPYLGK----CQICGVQGHSAKRCSQLQHFLSSVNSQQPPSPFTPWQPRANLALGSP 329

Query: 114 -SDQNLYADSGATSHVLNDAGKLASVKTYKGKESIYIGDGNVLEISHIRHSKLSTDFGNL 173
            S  N   DSGAT H+ +D   L+  + Y G + + + DG+ + ISH   + LST    L
Sbjct: 330 YSSNNWLLDSGATHHITSDFNNLSLHQPYTGGDDVMVADGSTIPISHTGSTSLSTKSRPL 389

Query: 174 VLKDVLDVPNMTKK-SSLSKLAKDNDCVITFSTDNFVVKN-KQGEILAKGHKKGGLY 220
            L ++L VPN+ K   S+ +L   N   + F   +F VK+   G  L +G  K  LY
Sbjct: 390 NLHNILYVPNIHKNLISVYRLCNANGVSVEFFPASFQVKDLNTGVPLLQGKTKDELY 442

BLAST of MC07g_new0035 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 3.4e-09
Identity = 53/167 (31.74%), Postives = 74/167 (44.31%), Query Frame = 0

Query: 67  CQICGKFNHTAIECWNRFDHSFQLED------------LPKALAAFNLKDTSDQNLYADS 126
           CQIC    H+A  C     H FQ                P+A  A N    +  N   DS
Sbjct: 258 CQICSVQGHSAKRCPQL--HQFQSTTNQQQSTSPFTPWQPRANLAVN-SPYNANNWLLDS 317

Query: 127 GATSHVLNDAGKLASVKTYKGKESIYIGDGNVLEISHIRHSKLSTDFGNLVLKDVLDVPN 186
           GAT H+ +D   L+  + Y G + + I DG+ + I+H   + L T   +L L  VL VPN
Sbjct: 318 GATHHITSDFNNLSFHQPYTGGDDVMIADGSTIPITHTGSASLPTSSRSLDLNKVLYVPN 377

Query: 187 MTKK-SSLSKLAKDNDCVITFSTDNFVVKN-KQGEILAKGHKKGGLY 220
           + K   S+ +L   N   + F   +F VK+   G  L +G  K  LY
Sbjct: 378 IHKNLISVYRLCNTNRVSVEFFPASFQVKDLNTGVPLLQGKTKDELY 421

BLAST of MC07g_new0035 vs. NCBI nr
Match: TXG63956.1 (hypothetical protein EZV62_010950 [Acer yangbiense])

HSP 1 Score: 205 bits (521), Expect = 8.48e-58
Identity = 101/165 (61.21%), Postives = 125/165 (75.76%), Query Frame = 0

Query: 60  KAEGRVSCQICGKFNHTAIECWNRFDHSFQLED-LPKALAAFNLKDTSDQNLYADSGATS 119
           + E  ++CQICGKFNHTA+ECWNRFDHS+Q ED LPKALAA NLK+  D NLYADSGAT+
Sbjct: 106 QGESTMTCQICGKFNHTALECWNRFDHSYQPEDHLPKALAAMNLKNKDDPNLYADSGATT 165

Query: 120 HVLNDAGKLASVKTYKGKESIYIGDGNVLEISHIRHSKLSTDFGNLVLKDVLDVPNMTKK 179
           H+LND GKL+ V  YKG ++I +G+G  L ISHI   K+ T  G L LK+VL VPN+ K 
Sbjct: 166 HILNDPGKLSKVMRYKGNDTILVGNGESLNISHIGEGKIETKDGTLDLKNVLIVPNIKKN 225

Query: 180 S-SLSKLAKDNDCVITFSTDNFVVKNKQGEILAKGHKKGGLYALD 222
             S+ +LA DN C I F+ +NF VK++QG+I+AKG K GGLYALD
Sbjct: 226 LISVGQLASDNACTIEFNANNFFVKSQQGKIIAKGRKNGGLYALD 270

BLAST of MC07g_new0035 vs. NCBI nr
Match: RVW43526.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera])

HSP 1 Score: 186 bits (471), Expect = 6.17e-50
Identity = 106/251 (42.23%), Postives = 155/251 (61.75%), Query Frame = 0

Query: 1   MDHNQALYSQRGRNKGRGRHFSSIGRGFQQ-GRPHIMQQPDLKQS--------------- 60
           ++H QA ++QRGR + RG  F S GRGF   GR +     D +Q+               
Sbjct: 264 INHEQAYFTQRGRGRNRGGRFLSRGRGFTPAGRFNNNATSDQRQNVYSSTRNPRNSFNNQ 323

Query: 61  --PQRLQTE--KTNENSPF-------KAEGRVSCQICGKFNHTAIECWNRFDHSFQLEDL 120
             PQ++ T   + + N+ F       K+EG + CQIC K NH+A++CWNRFD+ +QLE++
Sbjct: 324 FPPQQISTRNPRNSFNNQFPPQQKLQKSEGTLICQICSKANHSALDCWNRFDYLYQLEEI 383

Query: 121 PKALAAFNL-KDTSDQNLYADSGATSHVLNDAGKLASVKTYKGKESIYIGDGNVLEISHI 180
           P+ALAA  L ++  D N Y DSGAT+H+ ND GK++ V  YKG ++I++G+G  L ISHI
Sbjct: 384 PQALAAMALHEEEKDPNFYVDSGATAHITNDPGKMSQVIPYKGHDAIFVGNGEALRISHI 443

Query: 181 RHSKLSTDFGNLVLKDVLDVPNMTKKS-SLSKLAKDNDCVITFSTDNFVVKNKQGEILAK 222
             ++L T   +L LK +L VP + K   S+ +L  DN C I FS+  FV+K++  ++LA+
Sbjct: 444 GEARLKTKHRDLKLKKLLVVPEIKKNLLSIGQLTSDNACSIEFSSTGFVIKDQLQQVLAR 503

BLAST of MC07g_new0035 vs. NCBI nr
Match: PNY13708.1 (hypothetical protein L195_g010371 [Trifolium pratense])

HSP 1 Score: 183 bits (464), Expect = 3.77e-49
Identity = 101/229 (44.10%), Postives = 139/229 (60.70%), Query Frame = 0

Query: 1   MDHNQALYSQRGRNKGR----GR-HFSSIGRGFQQ-GRPHIMQQPDLKQSPQRLQTEKTN 60
           M+H QA +SQR R +GR    GR HF+S GRGF   GR +  Q  +      R    + +
Sbjct: 241 MEHAQAFFSQRARGRGRSARGGRGHFNSQGRGFTPAGRYNRQQNNEFFFKHNRNSKGEKS 300

Query: 61  ENSPFKAEGRVSCQICGKFNHTAIECWNRFDHSFQLEDLPKALAAFNLKDTSDQNLYADS 120
             +    E    CQIC K NH A++CW R+D+S+Q ED P+A AA  L DT DQ+   DS
Sbjct: 301 RPTTQPNESNSDCQICKKPNHEAVDCWYRYDYSYQSEDFPQAFAALALNDTKDQSFIVDS 360

Query: 121 GATSHVLNDAGKLASVKTYKGKESIYIGDGNVLEISHIRHSKLSTDFGNLVLKDVLDVPN 180
           GATSH++NDAG L+ VK Y+G + IY+GDGN + I+H     + +  GNL LKDVL VP+
Sbjct: 361 GATSHMINDAGNLSYVKPYRGNDVIYVGDGNSIPITHTGDVNVISTTGNLKLKDVLVVPD 420

Query: 181 MTKKS-SLSKLAKDNDCVITFSTDNFVVKNKQGEILAKGHKKGGLYALD 222
           +     S+ K   DN C   F++  FV+K++   ++A+GHKKG LY L+
Sbjct: 421 LKNNLLSVGKFVSDNHCSFEFTSSGFVIKDQNQRMIARGHKKGQLYTLE 469

BLAST of MC07g_new0035 vs. NCBI nr
Match: RVW77775.1 (Retrovirus-related Pol polyprotein from transposon RE2 [Vitis vinifera])

HSP 1 Score: 182 bits (461), Expect = 1.16e-48
Identity = 104/251 (41.43%), Postives = 154/251 (61.35%), Query Frame = 0

Query: 1   MDHNQALYSQRGRNKGRGRHFSSIGRGFQQ-GRPHIMQQPDLKQS--------------- 60
           ++H Q+ ++QRGR + RG  F S GRGF   GR +     D +Q+               
Sbjct: 193 INHEQSYFTQRGRGRNRGGRFLSRGRGFTPAGRFNNNATSDQRQNVHSSTRNPRNIFNNQ 252

Query: 61  --PQRLQTE--KTNENSPF-------KAEGRVSCQICGKFNHTAIECWNRFDHSFQLEDL 120
             PQ++ T   + + N+ F       K+EG + CQIC K NH+A++CWNRFD+S+Q E++
Sbjct: 253 FPPQQINTRNPRNSFNNQFPPQQKLQKSEGTLICQICSKANHSALDCWNRFDYSYQSEEI 312

Query: 121 PKALAAFNL-KDTSDQNLYADSGATSHVLNDAGKLASVKTYKGKESIYIGDGNVLEISHI 180
           P+ALAA  L ++  D N Y DSGAT+H+ ND  K++ V  YKG ++I++G+G  L ISHI
Sbjct: 313 PQALAAMALHEEEKDPNFYVDSGATAHITNDPSKMSQVIPYKGHDAIFVGNGEALRISHI 372

Query: 181 RHSKLSTDFGNLVLKDVLDVPNMTKKS-SLSKLAKDNDCVITFSTDNFVVKNKQGEILAK 222
             ++L T   +L LK +L VP + K   S+ +L  DN C I FS+  FV+K++  ++LA+
Sbjct: 373 GEARLKTKHRDLKLKKLLVVPEIKKNLLSIRQLTSDNACSIEFSSTGFVIKDQLQQVLAR 432

BLAST of MC07g_new0035 vs. NCBI nr
Match: TXG67661.1 (hypothetical protein EZV62_008936 [Acer yangbiense])

HSP 1 Score: 175 bits (444), Expect = 1.27e-46
Identity = 101/246 (41.06%), Postives = 141/246 (57.32%), Query Frame = 0

Query: 1   MDHNQALYSQRGRN-------------KGRGRHFSSIGR-----GFQQGRPHIMQQPDLK 60
           ++HNQA +SQRGR                RG+ F+  GR     G Q        QP+  
Sbjct: 344 IEHNQAFFSQRGRRGRNGRGAGGRVQLNSRGKGFTPAGRYNTQNGGQNSGQQNSNQPNNN 403

Query: 61  QSPQRLQTEKTNE-----NSPFKAEGRVSCQICGKFNHTAIECWNRFDHSFQLEDLPKAL 120
              Q+ Q     +     N+P   +G++ CQICGK NH AI+CW RF++S+Q E+ P+ L
Sbjct: 404 LGHQQKQGTTQGQPQWRPNAPNN-DGKIVCQICGKMNHFAIDCWYRFNYSYQSEEFPQDL 463

Query: 121 AAFNLKDTSDQNLYADSGATSHVLNDAGKLASVKTYKGKESIYIGDGNVLEISHIRHSKL 180
           A   L + +D + YADS AT+H+ NDAGKL+S++ Y G + IY+GDGN   ISHI  + +
Sbjct: 464 ATLTLHNDNDPSFYADSRATTHMTNDAGKLSSIQRYDGNDMIYVGDGNGSPISHIDDAHV 523

Query: 181 STDFGNLVLKDVLDVPNMTKKSS-LSKLAKDNDCVITFSTDNFVVKNKQGEILAKGHKKG 222
           +T  G   L DVL VP + K    + K   DN C   F++ +FVVK++   I+A+GHKKG
Sbjct: 524 TTHEGMFKLNDVLIVPELKKNLLFVGKFTSDNSCTFEFTSSDFVVKDRNQRIIARGHKKG 583

BLAST of MC07g_new0035 vs. ExPASy TrEMBL
Match: A0A5C7I5Y2 (gag_pre-integrs domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_010950 PE=4 SV=1)

HSP 1 Score: 205 bits (521), Expect = 4.10e-58
Identity = 101/165 (61.21%), Postives = 125/165 (75.76%), Query Frame = 0

Query: 60  KAEGRVSCQICGKFNHTAIECWNRFDHSFQLED-LPKALAAFNLKDTSDQNLYADSGATS 119
           + E  ++CQICGKFNHTA+ECWNRFDHS+Q ED LPKALAA NLK+  D NLYADSGAT+
Sbjct: 106 QGESTMTCQICGKFNHTALECWNRFDHSYQPEDHLPKALAAMNLKNKDDPNLYADSGATT 165

Query: 120 HVLNDAGKLASVKTYKGKESIYIGDGNVLEISHIRHSKLSTDFGNLVLKDVLDVPNMTKK 179
           H+LND GKL+ V  YKG ++I +G+G  L ISHI   K+ T  G L LK+VL VPN+ K 
Sbjct: 166 HILNDPGKLSKVMRYKGNDTILVGNGESLNISHIGEGKIETKDGTLDLKNVLIVPNIKKN 225

Query: 180 S-SLSKLAKDNDCVITFSTDNFVVKNKQGEILAKGHKKGGLYALD 222
             S+ +LA DN C I F+ +NF VK++QG+I+AKG K GGLYALD
Sbjct: 226 LISVGQLASDNACTIEFNANNFFVKSQQGKIIAKGRKNGGLYALD 270

BLAST of MC07g_new0035 vs. ExPASy TrEMBL
Match: A0A438E6Z5 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_1575 PE=4 SV=1)

HSP 1 Score: 186 bits (471), Expect = 2.99e-50
Identity = 106/251 (42.23%), Postives = 155/251 (61.75%), Query Frame = 0

Query: 1   MDHNQALYSQRGRNKGRGRHFSSIGRGFQQ-GRPHIMQQPDLKQS--------------- 60
           ++H QA ++QRGR + RG  F S GRGF   GR +     D +Q+               
Sbjct: 264 INHEQAYFTQRGRGRNRGGRFLSRGRGFTPAGRFNNNATSDQRQNVYSSTRNPRNSFNNQ 323

Query: 61  --PQRLQTE--KTNENSPF-------KAEGRVSCQICGKFNHTAIECWNRFDHSFQLEDL 120
             PQ++ T   + + N+ F       K+EG + CQIC K NH+A++CWNRFD+ +QLE++
Sbjct: 324 FPPQQISTRNPRNSFNNQFPPQQKLQKSEGTLICQICSKANHSALDCWNRFDYLYQLEEI 383

Query: 121 PKALAAFNL-KDTSDQNLYADSGATSHVLNDAGKLASVKTYKGKESIYIGDGNVLEISHI 180
           P+ALAA  L ++  D N Y DSGAT+H+ ND GK++ V  YKG ++I++G+G  L ISHI
Sbjct: 384 PQALAAMALHEEEKDPNFYVDSGATAHITNDPGKMSQVIPYKGHDAIFVGNGEALRISHI 443

Query: 181 RHSKLSTDFGNLVLKDVLDVPNMTKKS-SLSKLAKDNDCVITFSTDNFVVKNKQGEILAK 222
             ++L T   +L LK +L VP + K   S+ +L  DN C I FS+  FV+K++  ++LA+
Sbjct: 444 GEARLKTKHRDLKLKKLLVVPEIKKNLLSIGQLTSDNACSIEFSSTGFVIKDQLQQVLAR 503

BLAST of MC07g_new0035 vs. ExPASy TrEMBL
Match: A0A2K3PEI3 (Reverse transcriptase Ty1/copia-type domain-containing protein OS=Trifolium pratense OX=57577 GN=L195_g010371 PE=4 SV=1)

HSP 1 Score: 183 bits (464), Expect = 1.83e-49
Identity = 101/229 (44.10%), Postives = 139/229 (60.70%), Query Frame = 0

Query: 1   MDHNQALYSQRGRNKGR----GR-HFSSIGRGFQQ-GRPHIMQQPDLKQSPQRLQTEKTN 60
           M+H QA +SQR R +GR    GR HF+S GRGF   GR +  Q  +      R    + +
Sbjct: 241 MEHAQAFFSQRARGRGRSARGGRGHFNSQGRGFTPAGRYNRQQNNEFFFKHNRNSKGEKS 300

Query: 61  ENSPFKAEGRVSCQICGKFNHTAIECWNRFDHSFQLEDLPKALAAFNLKDTSDQNLYADS 120
             +    E    CQIC K NH A++CW R+D+S+Q ED P+A AA  L DT DQ+   DS
Sbjct: 301 RPTTQPNESNSDCQICKKPNHEAVDCWYRYDYSYQSEDFPQAFAALALNDTKDQSFIVDS 360

Query: 121 GATSHVLNDAGKLASVKTYKGKESIYIGDGNVLEISHIRHSKLSTDFGNLVLKDVLDVPN 180
           GATSH++NDAG L+ VK Y+G + IY+GDGN + I+H     + +  GNL LKDVL VP+
Sbjct: 361 GATSHMINDAGNLSYVKPYRGNDVIYVGDGNSIPITHTGDVNVISTTGNLKLKDVLVVPD 420

Query: 181 MTKKS-SLSKLAKDNDCVITFSTDNFVVKNKQGEILAKGHKKGGLYALD 222
           +     S+ K   DN C   F++  FV+K++   ++A+GHKKG LY L+
Sbjct: 421 LKNNLLSVGKFVSDNHCSFEFTSSGFVIKDQNQRMIARGHKKGQLYTLE 469

BLAST of MC07g_new0035 vs. ExPASy TrEMBL
Match: A0A438GZV0 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Vitis vinifera OX=29760 GN=RE2_55 PE=4 SV=1)

HSP 1 Score: 182 bits (461), Expect = 5.62e-49
Identity = 104/251 (41.43%), Postives = 154/251 (61.35%), Query Frame = 0

Query: 1   MDHNQALYSQRGRNKGRGRHFSSIGRGFQQ-GRPHIMQQPDLKQS--------------- 60
           ++H Q+ ++QRGR + RG  F S GRGF   GR +     D +Q+               
Sbjct: 193 INHEQSYFTQRGRGRNRGGRFLSRGRGFTPAGRFNNNATSDQRQNVHSSTRNPRNIFNNQ 252

Query: 61  --PQRLQTE--KTNENSPF-------KAEGRVSCQICGKFNHTAIECWNRFDHSFQLEDL 120
             PQ++ T   + + N+ F       K+EG + CQIC K NH+A++CWNRFD+S+Q E++
Sbjct: 253 FPPQQINTRNPRNSFNNQFPPQQKLQKSEGTLICQICSKANHSALDCWNRFDYSYQSEEI 312

Query: 121 PKALAAFNL-KDTSDQNLYADSGATSHVLNDAGKLASVKTYKGKESIYIGDGNVLEISHI 180
           P+ALAA  L ++  D N Y DSGAT+H+ ND  K++ V  YKG ++I++G+G  L ISHI
Sbjct: 313 PQALAAMALHEEEKDPNFYVDSGATAHITNDPSKMSQVIPYKGHDAIFVGNGEALRISHI 372

Query: 181 RHSKLSTDFGNLVLKDVLDVPNMTKKS-SLSKLAKDNDCVITFSTDNFVVKNKQGEILAK 222
             ++L T   +L LK +L VP + K   S+ +L  DN C I FS+  FV+K++  ++LA+
Sbjct: 373 GEARLKTKHRDLKLKKLLVVPEIKKNLLSIRQLTSDNACSIEFSSTGFVIKDQLQQVLAR 432

BLAST of MC07g_new0035 vs. ExPASy TrEMBL
Match: A0A5C7IEW8 (LRRNT_2 domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_008936 PE=4 SV=1)

HSP 1 Score: 175 bits (444), Expect = 6.14e-47
Identity = 101/246 (41.06%), Postives = 141/246 (57.32%), Query Frame = 0

Query: 1   MDHNQALYSQRGRN-------------KGRGRHFSSIGR-----GFQQGRPHIMQQPDLK 60
           ++HNQA +SQRGR                RG+ F+  GR     G Q        QP+  
Sbjct: 344 IEHNQAFFSQRGRRGRNGRGAGGRVQLNSRGKGFTPAGRYNTQNGGQNSGQQNSNQPNNN 403

Query: 61  QSPQRLQTEKTNE-----NSPFKAEGRVSCQICGKFNHTAIECWNRFDHSFQLEDLPKAL 120
              Q+ Q     +     N+P   +G++ CQICGK NH AI+CW RF++S+Q E+ P+ L
Sbjct: 404 LGHQQKQGTTQGQPQWRPNAPNN-DGKIVCQICGKMNHFAIDCWYRFNYSYQSEEFPQDL 463

Query: 121 AAFNLKDTSDQNLYADSGATSHVLNDAGKLASVKTYKGKESIYIGDGNVLEISHIRHSKL 180
           A   L + +D + YADS AT+H+ NDAGKL+S++ Y G + IY+GDGN   ISHI  + +
Sbjct: 464 ATLTLHNDNDPSFYADSRATTHMTNDAGKLSSIQRYDGNDMIYVGDGNGSPISHIDDAHV 523

Query: 181 STDFGNLVLKDVLDVPNMTKKSS-LSKLAKDNDCVITFSTDNFVVKNKQGEILAKGHKKG 222
           +T  G   L DVL VP + K    + K   DN C   F++ +FVVK++   I+A+GHKKG
Sbjct: 524 TTHEGMFKLNDVLIVPELKKNLLFVGKFTSDNSCTFEFTSSDFVVKDRNQRIIARGHKKG 583