MC07g0705 (gene) Bitter gourd (Dali-11) v1

BLAST of MC07g0705 vs. ExPASy Swiss-Prot
Match: Q40541 (Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097 GN=NPK1 PE=1 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 2.0e-224
Identity = 420/670 (62.69%), Postives = 499/670 (74.48%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLTPDGDD------GGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPP 60
           MQDF GSVRRSLVF      D      G  FGG VEK+GSSIRKS  G+FSK  V ALP 
Sbjct: 1   MQDFIGSVRRSLVFKQSGDFDTGAAGVGSGFGGFVEKLGSSIRKSSIGIFSKAHVPALPS 60

Query: 61  V---------MKEDAPPIWWRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASR 120
           +          K+D PPI WR+GE+IGCGAFGRVYMGMN+DSGELLA+K+VSIA N ASR
Sbjct: 61  ISKAELPAKARKDDTPPIRWRKGEMIGCGAFGRVYMGMNVDSGELLAIKEVSIAMNGASR 120

Query: 121 EKAQAHIRELEEEVKLLKNLSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSF 180
           E+AQAH+RELEEEV LLKNLSHPNIVRYLGT R   SLNILLEFVPGGSISSLLGKFGSF
Sbjct: 121 ERAQAHVRELEEEVNLLKNLSHPNIVRYLGTAREAGSLNILLEFVPGGSISSLLGKFGSF 180

Query: 181 PESVIRTYTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATIN 240
           PESVIR YTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELAT+ 
Sbjct: 181 PESVIRMYTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATMT 240

Query: 241 GAKSMKGTPYWMAPEVILQTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGN 300
           GAKSMKGTPYWMAPEVILQTGHSF ADIWSVGCT+IEMATGKPPWSQQYQEVAALFHIG 
Sbjct: 241 GAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTIIEMATGKPPWSQQYQEVAALFHIGT 300

Query: 301 TKSHPPIPEHLSAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVES 360
           TKSHPPIPEHLSAE+KDFLLKCLQ+EP+ R +AS+LLQHPFV+ E+Q+    +R+S + +
Sbjct: 301 TKSHPPIPEHLSAESKDFLLKCLQKEPHLRHSASNLLQHPFVTAEHQEARPFLRSSFMGN 360

Query: 361 GKQMVGSGLNSNDLANSTI--QRSTCVGLKDICEMGSLRCSSVYSGN-YTSQSNWGSSNF 420
            + M    +   D+  S I   R++C GLKD+C + ++RCS+VY  N    +S W   N 
Sbjct: 361 PENMAAQRM---DVRTSIIPDMRASCNGLKDVCGVSAVRCSTVYPENSLGKESLWKLGNS 420

Query: 421 EEDMCQIDDKDLLVSPSIKYKSIISSSDLNKSFNPMCEPTDDDWACKLDGSSELGGNLMD 480
           ++DMCQ+D+ D +   S+K  S + S    KSFNPMCEP D+DW CK D S EL  +  +
Sbjct: 421 DDDMCQMDNDDFMFGASVKCSSDLHSPANYKSFNPMCEP-DNDWPCKFDESPELTKSQAN 480

Query: 481 LSGIQTSQETT------CKEN-DFTFPCGQSAVEDDEEVTESKIRAFLDEKAIELKKMQT 540
           L   Q + + T       KE+  FTFP GQSA EDD+E+TESKIRAFLDEKA++LKK+QT
Sbjct: 481 LHYDQATIKPTNNPIMSYKEDLAFTFPSGQSAAEDDDELTESKIRAFLDEKAMDLKKLQT 540

Query: 541 PLYEEFRSTINAANALGAVGTENSKSVSNFLKLPPKCGSPSKLRGKRLSSIDASNCS--- 600
           PLYE F +++N ++    VGT N ++V + + LPPK  SP ++  +RLS+     C+   
Sbjct: 541 PLYEGFYNSLNVSSTPSPVGTGNKENVPSNINLPPKSRSPKRMLSRRLSTAIEGACAPSP 600

Query: 601 -SRQSRIKQASILEDRALQEIQSPQLGEWNKLLHTQPESSTPSFSERQRKWKEELEEELK 642
            +   RI     L   A+QE Q P+  EW  LL +Q E+   SFSERQR+WKEEL+EEL+
Sbjct: 601 VTHSKRISNIGGLNGEAIQEAQLPRHNEWKDLLGSQREAVNSSFSERQRRWKEELDEELQ 660

BLAST of MC07g0705 vs. ExPASy Swiss-Prot
Match: O22042 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=ANP3 PE=1 SV=1)

HSP 1 Score: 694.1 bits (1790), Expect = 1.5e-198
Identity = 386/647 (59.66%), Postives = 460/647 (71.10%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLT----PDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVM 60
           MQD  GSVRRSLVF +     DG  GG   G V KI SSIR SR GLFSK     LP   
Sbjct: 1   MQDILGSVRRSLVFRSSLAGDDGTSGGGLSGFVGKINSSIRSSRIGLFSK-PPPGLPAPR 60

Query: 61  KEDAPPIWWRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELE 120
           KE+AP I WR+GELIGCGAFGRVYMGMNLDSGELLA+KQV IA +SAS+EK Q HIRELE
Sbjct: 61  KEEAPSIRWRKGELIGCGAFGRVYMGMNLDSGELLAIKQVLIAPSSASKEKTQGHIRELE 120

Query: 121 EEVKLLKNLSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQ 180
           EEV+LLKNLSHPNIVRYLGT R  DSLNIL+EFVPGGSISSLL KFGSFPE VI  YTKQ
Sbjct: 121 EEVQLLKNLSHPNIVRYLGTVRESDSLNILMEFVPGGSISSLLEKFGSFPEPVIIMYTKQ 180

Query: 181 LLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYW 240
           LLLGLEYLH NGIMHRDIKGANILVDNKGCI+LADFGASKKVVELAT+NGAKSMKGTPYW
Sbjct: 181 LLLGLEYLHNNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATVNGAKSMKGTPYW 240

Query: 241 MAPEVILQTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHL 300
           MAPEVILQTGHSF ADIWSVGCTVIEMATGKPPWS+QYQ+ AA+ HIG TK+HPPIPE L
Sbjct: 241 MAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTKAHPPIPEDL 300

Query: 301 SAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMVGSGLNS 360
           S EAKDFL+KCL +EP+ R +A++LLQHPFV+ + Q+P+ A R S  E G  +   G+N 
Sbjct: 301 SPEAKDFLMKCLHKEPSLRLSATELLQHPFVTGKRQEPYPAYRNSLTECGNPITTQGMNV 360

Query: 361 NDLANSTIQRSTCVGLKDICEMGSLRCSSVYSGNYTSQSNWGSSNFEEDMCQIDDKDLLV 420
               NS I+RSTC GLKD+CE+GSLR S +Y     +          +D+CQ D  DL  
Sbjct: 361 RSSINSLIRRSTCSGLKDVCELGSLRSSIIYPQKSNNSGFGWRDGDSDDLCQTDMDDLCN 420

Query: 421 SPSIKYKSIISSSDLNKSFNPMCEPTDDDWACKLDGSSELGGNLMDLSGIQTSQETT--- 480
             S++   +  S+DLNKSFNPMC+ T D+W+CK D S ++  +  +L   Q SQ  T   
Sbjct: 421 IESVRNNVLSQSTDLNKSFNPMCDST-DNWSCKFDESPKVMKSKSNLLSYQASQLQTGVP 480

Query: 481 C-KENDFTFPCGQSAVEDDEEVTESKIRAFLDEKAIELKKMQTPLYEEFRSTINAANALG 540
           C +E   TF  G S  EDD + TE KI++FLDEKA +LK++QTPL EEF + +N     G
Sbjct: 481 CDEETSLTFAGGSSVAEDDYKGTELKIKSFLDEKAQDLKRLQTPLLEEFHNAMNPGIPQG 540

Query: 541 AVGTENSKSVSNFLKLPPKCGSPSKLRGKRLSSIDASNCSSRQSRIKQASILEDRALQEI 600
           A+G  N   + N   LP    +P +L  +RLS+I  +  S  +S   + ++   R +Q  
Sbjct: 541 ALGDTN---IYNLPNLPSISKTPKRLPSRRLSAISDAMPSPLKS--SKRTLNTSRVMQSG 600

Query: 601 QSP-QLGEWNKLLHTQPESSTPSFSERQRKWKEELEEELKRKREMMR 639
             P Q+ E  K    +  +++  FSE +RKW+EEL EEL+R RE +R
Sbjct: 601 TEPTQVNESTK----KGVNNSRCFSEIRRKWEEELYEELERHRENLR 636

BLAST of MC07g0705 vs. ExPASy Swiss-Prot
Match: O22040 (Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3702 GN=ANP1 PE=1 SV=2)

HSP 1 Score: 605.5 bits (1560), Expect = 6.9e-172
Identity = 368/667 (55.17%), Postives = 440/667 (65.97%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLTPDGDDGGRF-----GGLVEKIGSSIRKSRNGLFSKHSVRALPPV 60
           MQDFFGSVRRSLVF     DD         G L +KI S IRKS+  +F K S    PP 
Sbjct: 1   MQDFFGSVRRSLVFRPSSDDDNQENQPPFPGVLADKITSCIRKSK--IFIKPSFSPPPPA 60

Query: 61  MKED-APPIWWRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRE 120
              D APPI WR+G+LIG GAFG VYMGMNLDSGELLAVKQV IAAN AS+EK QAHI+E
Sbjct: 61  NTVDMAPPISWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQE 120

Query: 121 LEEEVKLLKNLSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYT 180
           LEEEVKLLKNLSHPNIVRYLGT R +D+LNILLEFVPGGSISSLL KFG FPESV+RTYT
Sbjct: 121 LEEEVKLLKNLSHPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYT 180

Query: 181 KQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTP 240
           +QLLLGLEYLH + IMHRDIKGANILVDNKGCIKLADFGASK+V ELAT+ GAKSMKGTP
Sbjct: 181 RQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTP 240

Query: 241 YWMAPEVILQTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPE 300
           YWMAPEVILQTGHSF ADIWSVGCTVIEM TGK PWSQQY+EVAA+F IG TKSHPPIP+
Sbjct: 241 YWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPD 300

Query: 301 HLSAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMVGSGL 360
            LS++AKDFLLKCLQE PN RPTAS+LL+HPFV  ++++  +    S + +    +   +
Sbjct: 301 TLSSDAKDFLLKCLQEVPNLRPTASELLKHPFVMGKHKESASTDLGSVLNNLSTPLPLQI 360

Query: 361 NSNDLANSTIQRSTCVGLKDICEMGSLRCS------SVYSGNYTSQSNWGSSNFEEDMCQ 420
           N+      +   STC  + D+C  GSL  S      S+ + N   Q++ G    E+DMC 
Sbjct: 361 NN----TKSTPDSTCDDVGDMCNFGSLNYSLVDPVKSIQNKNLWQQNDNGGD--EDDMCL 420

Query: 421 IDDKDLLVSPSIKYKSIISSSDLNKSFNPMCEPTDDDWACKLDGSSELGGNLMDLSGIQT 480
           IDD++ L        ++     L KS    C+   D          E  GN         
Sbjct: 421 IDDENFLTFDGEMSSTLEKDCHLKKS----CDDISDMSIALKSKFDESPGN--------- 480

Query: 481 SQETTCKENDFTFPCGQ-SAVEDDEEVTESKIRAFLDEKAIELKKMQTPLYEEF-RSTIN 540
                 KE+  +  C Q S  EDD+E+TESKI+AFLDEKA +LKK+QTPLYEEF  S I 
Sbjct: 481 ----GEKESTMSMECDQPSYSEDDDELTESKIKAFLDEKAADLKKLQTPLYEEFYNSLIT 540

Query: 541 AANALGAVGTENSK---SVSNFLKLPPKCGSPSK--LRGKRLSSIDASNCS-------SR 600
            + +       NSK   +   FLKLPPK  SPS+  L G    + DA++CS       SR
Sbjct: 541 FSPSCMESNLSNSKREDTARGFLKLPPKSRSPSRGPLGGSPSRATDATSCSKSPGSGGSR 600

Query: 601 QSRIKQASILEDRALQEIQSPQLGEWNKLL--HTQPESSTPSFSERQRKWKEELEEELKR 640
           +  I       D A Q+  S ++ +W  L+    Q  S   + SE ++KWKEEL++EL+R
Sbjct: 601 ELNINNGG---DEASQDGVSARVTDWRGLVVDTKQELSQCVALSEIEKKWKEELDQELER 639

BLAST of MC07g0705 vs. ExPASy Swiss-Prot
Match: Q9FZ36 (Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=ANP2 PE=2 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 2.0e-163
Identity = 346/659 (52.50%), Postives = 440/659 (66.77%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLTPDGDDGGR-----FGGLV-EKIGSSIRKSRNGLFSKHSVRALPP 60
           MQD FGSVRRSLVF +   D+        F  L+ +KI S IRKS   +F+K      PP
Sbjct: 1   MQDLFGSVRRSLVFRSTTDDENQENHPPPFPSLLADKITSCIRKSM--VFAKSQ---SPP 60

Query: 61  --VMKEDAPPIWWRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHI 120
                +  PPI WR+G+LIG GAFG VYMGMNLDSGELLAVKQV I +N AS+EK QAHI
Sbjct: 61  NNSTVQIKPPIRWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCASKEKTQAHI 120

Query: 121 RELEEEVKLLKNLSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRT 180
           +ELEEEVKLLKNLSHPNIVRYLGT R +++LNILLEFVPGGSISSLL KFG+FPESV+RT
Sbjct: 121 QELEEEVKLLKNLSHPNIVRYLGTVREDETLNILLEFVPGGSISSLLEKFGAFPESVVRT 180

Query: 181 YTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKG 240
           YT QLLLGLEYLH + IMHRDIKGANILVDN+GCIKLADFGASK+V ELATI+GAKSMKG
Sbjct: 181 YTNQLLLGLEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATISGAKSMKG 240

Query: 241 TPYWMAPEVILQTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPI 300
           TPYWMAPEVILQTGHSF ADIWSVGCTVIEM TGK PWSQQY+E+AA+FHIG TKSHPPI
Sbjct: 241 TPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKSHPPI 300

Query: 301 PEHLSAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMVGS 360
           P+++S++A DFLLKCLQ+EPN RPTAS+LL+HPFV+ + ++  +    S +++    + S
Sbjct: 301 PDNISSDANDFLLKCLQQEPNLRPTASELLKHPFVTGKQKESASKDLTSFMDNSCSPLPS 360

Query: 361 GLNSNDLANSTIQRSTCVGLKDICEMGSLRCS------SVYSGNYTSQSNWG-SSNFEED 420
            L +     ++ Q ST   + DIC +GSL C+      S+ + +   +SN G   + + D
Sbjct: 361 ELTN----ITSYQTSTSDDVGDICNLGSLTCTLAFPEKSIQNNSLCLKSNNGYDDDDDND 420

Query: 421 MCQIDDKDLLVSPSIKYKSIISSSDLNKSFNPMCEPTDDDWACKLDGSSELGGNLMDLSG 480
           MC IDD++ L        S+ +++D  KS + M E   D   CK D +S  G        
Sbjct: 421 MCLIDDENFLTYNGETGPSLDNNTDAKKSCDTMSE-ISDILKCKFDENSGNG-------- 480

Query: 481 IQTSQETTCKENDFTFPCGQSAVEDDEEVTESKIRAFLDEKAIELKKMQTPLYEEFRSTI 540
            +T  + + + +        S  ED+ E+TESKI+AFLD+KA ELKK+QTPLYEEF + +
Sbjct: 481 -ETETKVSMEVDH------PSYSEDENELTESKIKAFLDDKAAELKKLQTPLYEEFYNGM 540

Query: 541 NAANALGAVGTENS----KSVSNFLKLPPKCGSPSKLR-GKRLSSIDASNCSSRQSRIKQ 600
              + +      N+    ++   FLKLPPK  SPS+   G+  S    + C S+      
Sbjct: 541 ITCSPICMESNINNNKREEAPRGFLKLPPKSRSPSQGHIGRSPSRATDAACCSKSPESGN 600

Query: 601 ASILEDRALQEIQSPQLGEWNKLLHTQPESSTPSFSERQRKWKEELEEELKRKREMMRR 640
           +S           +P+    +     +  S + + SE +RKWKEEL++EL+RKR  + R
Sbjct: 601 SS----------GAPKNSNASAGAEQESNSQSVALSEIERKWKEELDQELERKRREITR 624

BLAST of MC07g0705 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 302.0 bits (772), Expect = 1.6e-80
Identity = 140/265 (52.83%), Postives = 194/265 (73.21%), Query Frame = 0

Query: 63  IWWRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVKLL 122
           I W++G+++G G +G VY+G+N D+GEL AVKQ+ I  +  S  K +  I    +E++++
Sbjct: 168 IKWQKGQILGRGGYGSVYLGLNKDTGELFAVKQLEI-VDINSDPKLKNMILSFSKEIEVM 227

Query: 123 KNLSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLGLE 182
           ++L H NIVRYLGT+  +  L++ LE++PGGSISSLLGKFG+F E+VI+ YTKQ+L GL 
Sbjct: 228 RSLRHDNIVRYLGTSLDQSFLSVFLEYIPGGSISSLLGKFGAFSENVIKVYTKQILQGLS 287

Query: 183 YLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPEVI 242
           +LH N I+HRDIKGANIL+D KG +KL+DFG SK    +  ++  KSM+GTPYWMAPEVI
Sbjct: 288 FLHANSIIHRDIKGANILIDTKGIVKLSDFGCSKSFSGI--VSQFKSMQGTPYWMAPEVI 347

Query: 243 LQTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEAKD 302
            QTGH   +DIWS+GC ++EMAT +PPWS   +  A ++HI ++ S P IP H+S EA D
Sbjct: 348 KQTGHGRSSDIWSLGCVIVEMATAQPPWSNITELAAVMYHIASSNSIPNIPSHMSQEAFD 407

Query: 303 FLLKCLQEEPNSRPTASDLLQHPFV 328
           FL  C + +P  RP A+ LL+HPF+
Sbjct: 408 FLNLCFKRDPKERPDANQLLKHPFI 429

BLAST of MC07g0705 vs. NCBI nr
Match: XP_022138818.1 (mitogen-activated protein kinase kinase kinase NPK1 [Momordica charantia])

HSP 1 Score: 1273 bits (3293), Expect = 0.0
Identity = 642/642 (100.00%), Postives = 642/642 (100.00%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLTPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVMKEDA 60
           MQDFFGSVRRSLVFLTPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVMKEDA
Sbjct: 1   MQDFFGSVRRSLVFLTPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVMKEDA 60

Query: 61  PPIWWRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMVGSGLNSNDLA 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMVGSGLNSNDLA
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMVGSGLNSNDLA 360

Query: 361 NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYTSQSNWGSSNFEEDMCQIDDKDLLVSPSI 420
           NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYTSQSNWGSSNFEEDMCQIDDKDLLVSPSI
Sbjct: 361 NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYTSQSNWGSSNFEEDMCQIDDKDLLVSPSI 420

Query: 421 KYKSIISSSDLNKSFNPMCEPTDDDWACKLDGSSELGGNLMDLSGIQTSQETTCKENDFT 480
           KYKSIISSSDLNKSFNPMCEPTDDDWACKLDGSSELGGNLMDLSGIQTSQETTCKENDFT
Sbjct: 421 KYKSIISSSDLNKSFNPMCEPTDDDWACKLDGSSELGGNLMDLSGIQTSQETTCKENDFT 480

Query: 481 FPCGQSAVEDDEEVTESKIRAFLDEKAIELKKMQTPLYEEFRSTINAANALGAVGTENSK 540
           FPCGQSAVEDDEEVTESKIRAFLDEKAIELKKMQTPLYEEFRSTINAANALGAVGTENSK
Sbjct: 481 FPCGQSAVEDDEEVTESKIRAFLDEKAIELKKMQTPLYEEFRSTINAANALGAVGTENSK 540

Query: 541 SVSNFLKLPPKCGSPSKLRGKRLSSIDASNCSSRQSRIKQASILEDRALQEIQSPQLGEW 600
           SVSNFLKLPPKCGSPSKLRGKRLSSIDASNCSSRQSRIKQASILEDRALQEIQSPQLGEW
Sbjct: 541 SVSNFLKLPPKCGSPSKLRGKRLSSIDASNCSSRQSRIKQASILEDRALQEIQSPQLGEW 600

Query: 601 NKLLHTQPESSTPSFSERQRKWKEELEEELKRKREMMRRGIV 642
           NKLLHTQPESSTPSFSERQRKWKEELEEELKRKREMMRRGIV
Sbjct: 601 NKLLHTQPESSTPSFSERQRKWKEELEEELKRKREMMRRGIV 642

BLAST of MC07g0705 vs. NCBI nr
Match: XP_023547660.1 (mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023547661.1 mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023547662.1 mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1174 bits (3036), Expect = 0.0
Identity = 589/642 (91.74%), Postives = 612/642 (95.33%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLTPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVMKEDA 60
           MQDFFGSVRRSLVFL PDGDDGG+FGGLVE+IGSSIRKSRNGLFSKHSVRALPPV KEDA
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PPIWWRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWR+GELIGCGAFGRVYMGMNLDSGEL+A+KQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGT R E SLNILLEFVPGGSISSLLGKFGSFPESV++TYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSF ADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMVGSGLNSNDLA 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQ+PHAAVRASS+ESGKQM  S LNSND+ 
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRASSLESGKQMADSRLNSNDIT 360

Query: 361 NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYTSQSNWGSSNFEEDMCQIDDKDLLVSPSI 420
            S IQRSTC GLKDICEMGSLRCSSV+SGNY  +SNWGSSNFE+DMCQIDDKDL  S SI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKDLFASSSI 420

Query: 421 KYKSIISSSDLNKSFNPMCEPTDDDWACKLDGSSELGGNLMDLSGIQTSQETTCKENDFT 480
           KYKSIISS+DLNKSFNPMCEPTDD WACKLD SSEL GNLM+LSGIQT QE+T K NDFT
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDD-WACKLDESSELRGNLMELSGIQTGQESTSKGNDFT 480

Query: 481 FPCGQSAVEDDEEVTESKIRAFLDEKAIELKKMQTPLYEEFRSTINAANALGAVGTENSK 540
           FPCGQS  EDDEEVT+SKI+AFLDEKA+ELKK+QTPLYEEFRST+NAANALGA GTENSK
Sbjct: 481 FPCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSK 540

Query: 541 SVSNFLKLPPKCGSPSKLRGKRLSSIDASNCSSRQSRIKQASILEDRALQEIQSPQLGEW 600
           SVSNFLKLPPKCGSPSKLRGKRLSSIDA NCSSRQSRIKQASIL+DRALQEIQSPQ+GEW
Sbjct: 541 SVSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEW 600

Query: 601 NKLLHTQPESSTPSFSERQRKWKEELEEELKRKREMMRRGIV 642
           NKLLHTQPES TPSFSERQRKWKEELEEELKRKREMMRRG V
Sbjct: 601 NKLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGTV 641

BLAST of MC07g0705 vs. NCBI nr
Match: XP_022958799.1 (mitogen-activated protein kinase kinase kinase NPK1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1169 bits (3025), Expect = 0.0
Identity = 586/642 (91.28%), Postives = 611/642 (95.17%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLTPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVMKEDA 60
           MQDFFGSVRRSLVFL PDGDDGG+FGGLVE+IGSSIRKSRNGLFSKHSVRALPPV KEDA
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PPIWWRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWR+GELIGCGAFGRVYMGMNLDSGEL+A+KQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGT R E SLNILLEFVPGGSISSLLGKFGSFPESV++TYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSF ADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMVGSGLNSNDLA 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQ+PHAAVRASS+ESGKQM G+ LNSND+ 
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRASSLESGKQMAGARLNSNDIT 360

Query: 361 NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYTSQSNWGSSNFEEDMCQIDDKDLLVSPSI 420
            S IQRSTC GLKDICEMGSLRCSSV+SGNY  +SNWGSSN E+DMCQIDDKDL  S SI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNSEDDMCQIDDKDLFASSSI 420

Query: 421 KYKSIISSSDLNKSFNPMCEPTDDDWACKLDGSSELGGNLMDLSGIQTSQETTCKENDFT 480
           KYKSIISS+DLNKSFNPMCEPTDD WACKLD SSEL GNLM+LSGIQT QE+T K NDFT
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDD-WACKLDESSELRGNLMELSGIQTGQESTSKGNDFT 480

Query: 481 FPCGQSAVEDDEEVTESKIRAFLDEKAIELKKMQTPLYEEFRSTINAANALGAVGTENSK 540
           FPCGQS  EDDEEVT+SKI+AFLDEKA+ELKK+QTPLYEEFRST+NAANALGA GTENSK
Sbjct: 481 FPCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSK 540

Query: 541 SVSNFLKLPPKCGSPSKLRGKRLSSIDASNCSSRQSRIKQASILEDRALQEIQSPQLGEW 600
           SVSNFLKLPPKCGSPSKLRGKRLSSID  NCSSRQSR+KQASIL+DRALQEIQSPQ+GEW
Sbjct: 541 SVSNFLKLPPKCGSPSKLRGKRLSSIDDGNCSSRQSRVKQASILQDRALQEIQSPQIGEW 600

Query: 601 NKLLHTQPESSTPSFSERQRKWKEELEEELKRKREMMRRGIV 642
           NKLLHTQPES TPSFSERQRKWKEELEEELKRKREMMRRG V
Sbjct: 601 NKLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGTV 641

BLAST of MC07g0705 vs. NCBI nr
Match: XP_023006461.1 (mitogen-activated protein kinase kinase kinase NPK1 isoform X2 [Cucurbita maxima])

HSP 1 Score: 1168 bits (3021), Expect = 0.0
Identity = 585/642 (91.12%), Postives = 611/642 (95.17%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLTPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVMKEDA 60
           MQDFFGSVRRSLVFL PDGDDGG+FGGLVE+IGSSIRKSRNGLFSKHSVRALPPV KEDA
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PPIWWRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWR+GELIGCGAFGRVYMGMNLDSGEL+A+KQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGT R E SLNILLEFVPGGSISSLLGKFGSFPESV++TYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSF ADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMVGSGLNSNDLA 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQ+PHAAVR SS+ESGKQM  S LNS D+ 
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRVSSLESGKQMADSRLNSKDIT 360

Query: 361 NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYTSQSNWGSSNFEEDMCQIDDKDLLVSPSI 420
            S IQRSTC GLKDICEMGSLRCSSV+SGNY  +SNWGSSNFE+DMCQIDDK+L  S SI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKNLFASSSI 420

Query: 421 KYKSIISSSDLNKSFNPMCEPTDDDWACKLDGSSELGGNLMDLSGIQTSQETTCKENDFT 480
           KYKSIISS+DLNKSFNPMCEPTDD WACKLD SSEL GNLM+LSGI+T QE+T K NDFT
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDD-WACKLDESSELRGNLMELSGIRTGQESTSKGNDFT 480

Query: 481 FPCGQSAVEDDEEVTESKIRAFLDEKAIELKKMQTPLYEEFRSTINAANALGAVGTENSK 540
           FPCGQS  EDDEEVT+SKI+AFLDEKA+ELKK+QTPLYEEFRST+NAANALGAVGTENSK
Sbjct: 481 FPCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAVGTENSK 540

Query: 541 SVSNFLKLPPKCGSPSKLRGKRLSSIDASNCSSRQSRIKQASILEDRALQEIQSPQLGEW 600
           SVSNFLKLPPKCGSPSKLRGKRLSSIDA NCSSRQSRIKQASIL+DRALQEIQSPQ+GEW
Sbjct: 541 SVSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEW 600

Query: 601 NKLLHTQPESSTPSFSERQRKWKEELEEELKRKREMMRRGIV 642
           NKLLHTQPES TP+FSERQRKWKEELEEELKRKREMMRRG V
Sbjct: 601 NKLLHTQPESCTPTFSERQRKWKEELEEELKRKREMMRRGTV 641

BLAST of MC07g0705 vs. NCBI nr
Match: KAG6575002.1 (Mitogen-activated protein kinase kinase kinase NPK1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013573.1 Mitogen-activated protein kinase kinase kinase NPK1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1165 bits (3015), Expect = 0.0
Identity = 585/642 (91.12%), Postives = 609/642 (94.86%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLTPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVMKEDA 60
           MQDFFGSVRRSLVFL PDGDDGG+FGGLVE+IGSSIRKSRNGLFSKHSVRALPPV KEDA
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PPIWWRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           P IWWR+GELIGCGAFGRVYMGMNLDSGEL+A+KQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PSIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGT R E SLNILLEFVPGGSISSLLGKFGSFPESV++TYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSF ADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMVGSGLNSNDLA 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQ+PHA VRASS+ESGKQM  S LNSND+ 
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAPVRASSLESGKQMADSRLNSNDIT 360

Query: 361 NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYTSQSNWGSSNFEEDMCQIDDKDLLVSPSI 420
            S IQRSTC GLKDICEMGSLRCSSV+SGNY  +SNWGSSNFE+DMCQIDDKDL  S SI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKDLFASSSI 420

Query: 421 KYKSIISSSDLNKSFNPMCEPTDDDWACKLDGSSELGGNLMDLSGIQTSQETTCKENDFT 480
           KYKSIISS+DLNKSFNPMCEPTDD W CKLD SSEL GNLM+LSGIQT QE+T K NDFT
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDD-WPCKLDESSELRGNLMELSGIQTGQESTSKGNDFT 480

Query: 481 FPCGQSAVEDDEEVTESKIRAFLDEKAIELKKMQTPLYEEFRSTINAANALGAVGTENSK 540
           FPCGQS  EDDEEVT+SKI+AFLDEKA+ELKK+QTPLYEEFRST+NAANALGA GTENSK
Sbjct: 481 FPCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSK 540

Query: 541 SVSNFLKLPPKCGSPSKLRGKRLSSIDASNCSSRQSRIKQASILEDRALQEIQSPQLGEW 600
           SVSNFLKLPPKCGSPSKLRGKRLSSIDA NCSSRQSRIKQASIL+DRALQEIQSPQ+GEW
Sbjct: 541 SVSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEW 600

Query: 601 NKLLHTQPESSTPSFSERQRKWKEELEEELKRKREMMRRGIV 642
           N+LLHTQPES TPSFSERQRKWKEELEEELKRKREMMRRG V
Sbjct: 601 NRLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGTV 641

BLAST of MC07g0705 vs. ExPASy TrEMBL
Match: A0A6J1CE75 (mitogen-activated protein kinase kinase kinase NPK1 OS=Momordica charantia OX=3673 GN=LOC111009894 PE=4 SV=1)

HSP 1 Score: 1273 bits (3293), Expect = 0.0
Identity = 642/642 (100.00%), Postives = 642/642 (100.00%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLTPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVMKEDA 60
           MQDFFGSVRRSLVFLTPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVMKEDA
Sbjct: 1   MQDFFGSVRRSLVFLTPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVMKEDA 60

Query: 61  PPIWWRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMVGSGLNSNDLA 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMVGSGLNSNDLA
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMVGSGLNSNDLA 360

Query: 361 NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYTSQSNWGSSNFEEDMCQIDDKDLLVSPSI 420
           NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYTSQSNWGSSNFEEDMCQIDDKDLLVSPSI
Sbjct: 361 NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYTSQSNWGSSNFEEDMCQIDDKDLLVSPSI 420

Query: 421 KYKSIISSSDLNKSFNPMCEPTDDDWACKLDGSSELGGNLMDLSGIQTSQETTCKENDFT 480
           KYKSIISSSDLNKSFNPMCEPTDDDWACKLDGSSELGGNLMDLSGIQTSQETTCKENDFT
Sbjct: 421 KYKSIISSSDLNKSFNPMCEPTDDDWACKLDGSSELGGNLMDLSGIQTSQETTCKENDFT 480

Query: 481 FPCGQSAVEDDEEVTESKIRAFLDEKAIELKKMQTPLYEEFRSTINAANALGAVGTENSK 540
           FPCGQSAVEDDEEVTESKIRAFLDEKAIELKKMQTPLYEEFRSTINAANALGAVGTENSK
Sbjct: 481 FPCGQSAVEDDEEVTESKIRAFLDEKAIELKKMQTPLYEEFRSTINAANALGAVGTENSK 540

Query: 541 SVSNFLKLPPKCGSPSKLRGKRLSSIDASNCSSRQSRIKQASILEDRALQEIQSPQLGEW 600
           SVSNFLKLPPKCGSPSKLRGKRLSSIDASNCSSRQSRIKQASILEDRALQEIQSPQLGEW
Sbjct: 541 SVSNFLKLPPKCGSPSKLRGKRLSSIDASNCSSRQSRIKQASILEDRALQEIQSPQLGEW 600

Query: 601 NKLLHTQPESSTPSFSERQRKWKEELEEELKRKREMMRRGIV 642
           NKLLHTQPESSTPSFSERQRKWKEELEEELKRKREMMRRGIV
Sbjct: 601 NKLLHTQPESSTPSFSERQRKWKEELEEELKRKREMMRRGIV 642

BLAST of MC07g0705 vs. ExPASy TrEMBL
Match: A0A6J1H661 (mitogen-activated protein kinase kinase kinase NPK1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459961 PE=4 SV=1)

HSP 1 Score: 1169 bits (3025), Expect = 0.0
Identity = 586/642 (91.28%), Postives = 611/642 (95.17%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLTPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVMKEDA 60
           MQDFFGSVRRSLVFL PDGDDGG+FGGLVE+IGSSIRKSRNGLFSKHSVRALPPV KEDA
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PPIWWRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWR+GELIGCGAFGRVYMGMNLDSGEL+A+KQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGT R E SLNILLEFVPGGSISSLLGKFGSFPESV++TYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSF ADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMVGSGLNSNDLA 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQ+PHAAVRASS+ESGKQM G+ LNSND+ 
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRASSLESGKQMAGARLNSNDIT 360

Query: 361 NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYTSQSNWGSSNFEEDMCQIDDKDLLVSPSI 420
            S IQRSTC GLKDICEMGSLRCSSV+SGNY  +SNWGSSN E+DMCQIDDKDL  S SI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNSEDDMCQIDDKDLFASSSI 420

Query: 421 KYKSIISSSDLNKSFNPMCEPTDDDWACKLDGSSELGGNLMDLSGIQTSQETTCKENDFT 480
           KYKSIISS+DLNKSFNPMCEPTDD WACKLD SSEL GNLM+LSGIQT QE+T K NDFT
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDD-WACKLDESSELRGNLMELSGIQTGQESTSKGNDFT 480

Query: 481 FPCGQSAVEDDEEVTESKIRAFLDEKAIELKKMQTPLYEEFRSTINAANALGAVGTENSK 540
           FPCGQS  EDDEEVT+SKI+AFLDEKA+ELKK+QTPLYEEFRST+NAANALGA GTENSK
Sbjct: 481 FPCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSK 540

Query: 541 SVSNFLKLPPKCGSPSKLRGKRLSSIDASNCSSRQSRIKQASILEDRALQEIQSPQLGEW 600
           SVSNFLKLPPKCGSPSKLRGKRLSSID  NCSSRQSR+KQASIL+DRALQEIQSPQ+GEW
Sbjct: 541 SVSNFLKLPPKCGSPSKLRGKRLSSIDDGNCSSRQSRVKQASILQDRALQEIQSPQIGEW 600

Query: 601 NKLLHTQPESSTPSFSERQRKWKEELEEELKRKREMMRRGIV 642
           NKLLHTQPES TPSFSERQRKWKEELEEELKRKREMMRRG V
Sbjct: 601 NKLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGTV 641

BLAST of MC07g0705 vs. ExPASy TrEMBL
Match: A0A6J1KXU2 (mitogen-activated protein kinase kinase kinase NPK1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499179 PE=4 SV=1)

HSP 1 Score: 1168 bits (3021), Expect = 0.0
Identity = 585/642 (91.12%), Postives = 611/642 (95.17%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLTPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVMKEDA 60
           MQDFFGSVRRSLVFL PDGDDGG+FGGLVE+IGSSIRKSRNGLFSKHSVRALPPV KEDA
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PPIWWRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWR+GELIGCGAFGRVYMGMNLDSGEL+A+KQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGT R E SLNILLEFVPGGSISSLLGKFGSFPESV++TYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSF ADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMVGSGLNSNDLA 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQ+PHAAVR SS+ESGKQM  S LNS D+ 
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRVSSLESGKQMADSRLNSKDIT 360

Query: 361 NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYTSQSNWGSSNFEEDMCQIDDKDLLVSPSI 420
            S IQRSTC GLKDICEMGSLRCSSV+SGNY  +SNWGSSNFE+DMCQIDDK+L  S SI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKNLFASSSI 420

Query: 421 KYKSIISSSDLNKSFNPMCEPTDDDWACKLDGSSELGGNLMDLSGIQTSQETTCKENDFT 480
           KYKSIISS+DLNKSFNPMCEPTDD WACKLD SSEL GNLM+LSGI+T QE+T K NDFT
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDD-WACKLDESSELRGNLMELSGIRTGQESTSKGNDFT 480

Query: 481 FPCGQSAVEDDEEVTESKIRAFLDEKAIELKKMQTPLYEEFRSTINAANALGAVGTENSK 540
           FPCGQS  EDDEEVT+SKI+AFLDEKA+ELKK+QTPLYEEFRST+NAANALGAVGTENSK
Sbjct: 481 FPCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAVGTENSK 540

Query: 541 SVSNFLKLPPKCGSPSKLRGKRLSSIDASNCSSRQSRIKQASILEDRALQEIQSPQLGEW 600
           SVSNFLKLPPKCGSPSKLRGKRLSSIDA NCSSRQSRIKQASIL+DRALQEIQSPQ+GEW
Sbjct: 541 SVSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEW 600

Query: 601 NKLLHTQPESSTPSFSERQRKWKEELEEELKRKREMMRRGIV 642
           NKLLHTQPES TP+FSERQRKWKEELEEELKRKREMMRRG V
Sbjct: 601 NKLLHTQPESCTPTFSERQRKWKEELEEELKRKREMMRRGTV 641

BLAST of MC07g0705 vs. ExPASy TrEMBL
Match: A0A6J1L4Z4 (mitogen-activated protein kinase kinase kinase NPK1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499179 PE=4 SV=1)

HSP 1 Score: 1163 bits (3009), Expect = 0.0
Identity = 585/643 (90.98%), Postives = 611/643 (95.02%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLTPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVMKEDA 60
           MQDFFGSVRRSLVFL PDGDDGG+FGGLVE+IGSSIRKSRNGLFSKHSVRALPPV KEDA
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PPIWWRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWR+GELIGCGAFGRVYMGMNLDSGEL+A+KQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGT R E SLNILLEFVPGGSISSLLGKFGSFPESV++TYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSF ADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMVGSGLNSNDLA 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQ+PHAAVR SS+ESGKQM  S LNS D+ 
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRVSSLESGKQMADSRLNSKDIT 360

Query: 361 NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYTSQSNWGSSNFEEDMCQIDDKDLLVSPSI 420
            S IQRSTC GLKDICEMGSLRCSSV+SGNY  +SNWGSSNFE+DMCQIDDK+L  S SI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKNLFASSSI 420

Query: 421 KYKSIISSSDLNKSFNPMCEPTDDDWACKLDGSSELGGNLMDLSGIQTSQETTCKENDFT 480
           KYKSIISS+DLNKSFNPMCEPTDD WACKLD SSEL GNLM+LSGI+T QE+T K NDFT
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDD-WACKLDESSELRGNLMELSGIRTGQESTSKGNDFT 480

Query: 481 FPCGQSAVEDDEEVTESKIRAFLDEKAIELKKMQTPLYEEFRSTINAANALGAVGTENSK 540
           FPCGQS  EDDEEVT+SKI+AFLDEKA+ELKK+QTPLYEEFRST+NAANALGAVGTENSK
Sbjct: 481 FPCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAVGTENSK 540

Query: 541 SVSNFLKLPPKCGSPSKLRGKRLSSIDASNCSSRQSRIKQASILEDRALQEIQSPQLGEW 600
           SVSNFLKLPPKCGSPSKLRGKRLSSIDA NCSSRQSRIKQASIL+DRALQEIQSPQ+GEW
Sbjct: 541 SVSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEW 600

Query: 601 NKLLHTQPESSTPSF-SERQRKWKEELEEELKRKREMMRRGIV 642
           NKLLHTQPES TP+F SERQRKWKEELEEELKRKREMMRRG V
Sbjct: 601 NKLLHTQPESCTPTFFSERQRKWKEELEEELKRKREMMRRGTV 642

BLAST of MC07g0705 vs. ExPASy TrEMBL
Match: A0A6J1H434 (mitogen-activated protein kinase kinase kinase NPK1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459961 PE=4 SV=1)

HSP 1 Score: 1163 bits (3008), Expect = 0.0
Identity = 585/643 (90.98%), Postives = 610/643 (94.87%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLTPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVMKEDA 60
           MQDFFGSVRRSLVFL PDGDDGG+FGGLVE+IGSSIRKSRNGLFSKHSVRALPPV KEDA
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PPIWWRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWR+GELIGCGAFGRVYMGMNLDSGEL+A+KQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGT R E SLNILLEFVPGGSISSLLGKFGSFPESV++TYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSF ADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMVGSGLNSNDLA 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQ+PHAAVRASS+ESGKQM G+ LNSND+ 
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRASSLESGKQMAGARLNSNDIT 360

Query: 361 NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYTSQSNWGSSNFEEDMCQIDDKDLLVSPSI 420
            S IQRSTC GLKDICEMGSLRCSSV+SGNY  +SNWGSSN E+DMCQIDDKDL  S SI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNSEDDMCQIDDKDLFASSSI 420

Query: 421 KYKSIISSSDLNKSFNPMCEPTDDDWACKLDGSSELGGNLMDLSGIQTSQETTCKENDFT 480
           KYKSIISS+DLNKSFNPMCEPTDD WACKLD SSEL GNLM+LSGIQT QE+T K NDFT
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDD-WACKLDESSELRGNLMELSGIQTGQESTSKGNDFT 480

Query: 481 FPCGQSAVEDDEEVTESKIRAFLDEKAIELKKMQTPLYEEFRSTINAANALGAVGTENSK 540
           FPCGQS  EDDEEVT+SKI+AFLDEKA+ELKK+QTPLYEEFRST+NAANALGA GTENSK
Sbjct: 481 FPCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSK 540

Query: 541 SVSNFLKLPPKCGSPSKLRGKRLSSIDASNCSSRQSRIKQASILEDRALQEIQSPQLGEW 600
           SVSNFLKLPPKCGSPSKLRGKRLSSID  NCSSRQSR+KQASIL+DRALQEIQSPQ+GEW
Sbjct: 541 SVSNFLKLPPKCGSPSKLRGKRLSSIDDGNCSSRQSRVKQASILQDRALQEIQSPQIGEW 600

Query: 601 NKLLHTQPESSTPSF-SERQRKWKEELEEELKRKREMMRRGIV 642
           NKLLHTQPES TP F SERQRKWKEELEEELKRKREMMRRG V
Sbjct: 601 NKLLHTQPESCTPRFFSERQRKWKEELEEELKRKREMMRRGTV 642

BLAST of MC07g0705 vs. TAIR 10
Match: AT3G06030.1 (NPK1-related protein kinase 3 )

HSP 1 Score: 694.1 bits (1790), Expect = 1.0e-199
Identity = 386/647 (59.66%), Postives = 460/647 (71.10%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLT----PDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVM 60
           MQD  GSVRRSLVF +     DG  GG   G V KI SSIR SR GLFSK     LP   
Sbjct: 1   MQDILGSVRRSLVFRSSLAGDDGTSGGGLSGFVGKINSSIRSSRIGLFSK-PPPGLPAPR 60

Query: 61  KEDAPPIWWRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELE 120
           KE+AP I WR+GELIGCGAFGRVYMGMNLDSGELLA+KQV IA +SAS+EK Q HIRELE
Sbjct: 61  KEEAPSIRWRKGELIGCGAFGRVYMGMNLDSGELLAIKQVLIAPSSASKEKTQGHIRELE 120

Query: 121 EEVKLLKNLSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQ 180
           EEV+LLKNLSHPNIVRYLGT R  DSLNIL+EFVPGGSISSLL KFGSFPE VI  YTKQ
Sbjct: 121 EEVQLLKNLSHPNIVRYLGTVRESDSLNILMEFVPGGSISSLLEKFGSFPEPVIIMYTKQ 180

Query: 181 LLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYW 240
           LLLGLEYLH NGIMHRDIKGANILVDNKGCI+LADFGASKKVVELAT+NGAKSMKGTPYW
Sbjct: 181 LLLGLEYLHNNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATVNGAKSMKGTPYW 240

Query: 241 MAPEVILQTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHL 300
           MAPEVILQTGHSF ADIWSVGCTVIEMATGKPPWS+QYQ+ AA+ HIG TK+HPPIPE L
Sbjct: 241 MAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTKAHPPIPEDL 300

Query: 301 SAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMVGSGLNS 360
           S EAKDFL+KCL +EP+ R +A++LLQHPFV+ + Q+P+ A R S  E G  +   G+N 
Sbjct: 301 SPEAKDFLMKCLHKEPSLRLSATELLQHPFVTGKRQEPYPAYRNSLTECGNPITTQGMNV 360

Query: 361 NDLANSTIQRSTCVGLKDICEMGSLRCSSVYSGNYTSQSNWGSSNFEEDMCQIDDKDLLV 420
               NS I+RSTC GLKD+CE+GSLR S +Y     +          +D+CQ D  DL  
Sbjct: 361 RSSINSLIRRSTCSGLKDVCELGSLRSSIIYPQKSNNSGFGWRDGDSDDLCQTDMDDLCN 420

Query: 421 SPSIKYKSIISSSDLNKSFNPMCEPTDDDWACKLDGSSELGGNLMDLSGIQTSQETT--- 480
             S++   +  S+DLNKSFNPMC+ T D+W+CK D S ++  +  +L   Q SQ  T   
Sbjct: 421 IESVRNNVLSQSTDLNKSFNPMCDST-DNWSCKFDESPKVMKSKSNLLSYQASQLQTGVP 480

Query: 481 C-KENDFTFPCGQSAVEDDEEVTESKIRAFLDEKAIELKKMQTPLYEEFRSTINAANALG 540
           C +E   TF  G S  EDD + TE KI++FLDEKA +LK++QTPL EEF + +N     G
Sbjct: 481 CDEETSLTFAGGSSVAEDDYKGTELKIKSFLDEKAQDLKRLQTPLLEEFHNAMNPGIPQG 540

Query: 541 AVGTENSKSVSNFLKLPPKCGSPSKLRGKRLSSIDASNCSSRQSRIKQASILEDRALQEI 600
           A+G  N   + N   LP    +P +L  +RLS+I  +  S  +S   + ++   R +Q  
Sbjct: 541 ALGDTN---IYNLPNLPSISKTPKRLPSRRLSAISDAMPSPLKS--SKRTLNTSRVMQSG 600

Query: 601 QSP-QLGEWNKLLHTQPESSTPSFSERQRKWKEELEEELKRKREMMR 639
             P Q+ E  K    +  +++  FSE +RKW+EEL EEL+R RE +R
Sbjct: 601 TEPTQVNESTK----KGVNNSRCFSEIRRKWEEELYEELERHRENLR 636

BLAST of MC07g0705 vs. TAIR 10
Match: AT1G09000.1 (NPK1-related protein kinase 1 )

HSP 1 Score: 605.5 bits (1560), Expect = 4.9e-173
Identity = 368/667 (55.17%), Postives = 440/667 (65.97%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLTPDGDDGGRF-----GGLVEKIGSSIRKSRNGLFSKHSVRALPPV 60
           MQDFFGSVRRSLVF     DD         G L +KI S IRKS+  +F K S    PP 
Sbjct: 1   MQDFFGSVRRSLVFRPSSDDDNQENQPPFPGVLADKITSCIRKSK--IFIKPSFSPPPPA 60

Query: 61  MKED-APPIWWRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRE 120
              D APPI WR+G+LIG GAFG VYMGMNLDSGELLAVKQV IAAN AS+EK QAHI+E
Sbjct: 61  NTVDMAPPISWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQE 120

Query: 121 LEEEVKLLKNLSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYT 180
           LEEEVKLLKNLSHPNIVRYLGT R +D+LNILLEFVPGGSISSLL KFG FPESV+RTYT
Sbjct: 121 LEEEVKLLKNLSHPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYT 180

Query: 181 KQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTP 240
           +QLLLGLEYLH + IMHRDIKGANILVDNKGCIKLADFGASK+V ELAT+ GAKSMKGTP
Sbjct: 181 RQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTP 240

Query: 241 YWMAPEVILQTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPE 300
           YWMAPEVILQTGHSF ADIWSVGCTVIEM TGK PWSQQY+EVAA+F IG TKSHPPIP+
Sbjct: 241 YWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPD 300

Query: 301 HLSAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMVGSGL 360
            LS++AKDFLLKCLQE PN RPTAS+LL+HPFV  ++++  +    S + +    +   +
Sbjct: 301 TLSSDAKDFLLKCLQEVPNLRPTASELLKHPFVMGKHKESASTDLGSVLNNLSTPLPLQI 360

Query: 361 NSNDLANSTIQRSTCVGLKDICEMGSLRCS------SVYSGNYTSQSNWGSSNFEEDMCQ 420
           N+      +   STC  + D+C  GSL  S      S+ + N   Q++ G    E+DMC 
Sbjct: 361 NN----TKSTPDSTCDDVGDMCNFGSLNYSLVDPVKSIQNKNLWQQNDNGGD--EDDMCL 420

Query: 421 IDDKDLLVSPSIKYKSIISSSDLNKSFNPMCEPTDDDWACKLDGSSELGGNLMDLSGIQT 480
           IDD++ L        ++     L KS    C+   D          E  GN         
Sbjct: 421 IDDENFLTFDGEMSSTLEKDCHLKKS----CDDISDMSIALKSKFDESPGN--------- 480

Query: 481 SQETTCKENDFTFPCGQ-SAVEDDEEVTESKIRAFLDEKAIELKKMQTPLYEEF-RSTIN 540
                 KE+  +  C Q S  EDD+E+TESKI+AFLDEKA +LKK+QTPLYEEF  S I 
Sbjct: 481 ----GEKESTMSMECDQPSYSEDDDELTESKIKAFLDEKAADLKKLQTPLYEEFYNSLIT 540

Query: 541 AANALGAVGTENSK---SVSNFLKLPPKCGSPSK--LRGKRLSSIDASNCS-------SR 600
            + +       NSK   +   FLKLPPK  SPS+  L G    + DA++CS       SR
Sbjct: 541 FSPSCMESNLSNSKREDTARGFLKLPPKSRSPSRGPLGGSPSRATDATSCSKSPGSGGSR 600

Query: 601 QSRIKQASILEDRALQEIQSPQLGEWNKLL--HTQPESSTPSFSERQRKWKEELEEELKR 640
           +  I       D A Q+  S ++ +W  L+    Q  S   + SE ++KWKEEL++EL+R
Sbjct: 601 ELNINNGG---DEASQDGVSARVTDWRGLVVDTKQELSQCVALSEIEKKWKEELDQELER 639

BLAST of MC07g0705 vs. TAIR 10
Match: AT1G54960.1 (NPK1-related protein kinase 2 )

HSP 1 Score: 555.1 bits (1429), Expect = 7.6e-158
Identity = 319/591 (53.98%), Postives = 406/591 (68.70%), Query Frame = 0

Query: 61  PPIWWRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPI WR+G+LIG GAFG VYMGMNLDSGELLAVKQV I +N AS+EK QAHI+ELEEEVK
Sbjct: 19  PPIRWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCASKEKTQAHIQELEEEVK 78

Query: 121 LLKNLSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGT R +++LNILLEFVPGGSISSLL KFG+FPESV+RTYT QLLLG
Sbjct: 79  LLKNLSHPNIVRYLGTVREDETLNILLEFVPGGSISSLLEKFGAFPESVVRTYTNQLLLG 138

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLH + IMHRDIKGANILVDN+GCIKLADFGASK+V ELATI+GAKSMKGTPYWMAPE
Sbjct: 139 LEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATISGAKSMKGTPYWMAPE 198

Query: 241 VILQTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSF ADIWSVGCTVIEM TGK PWSQQY+E+AA+FHIG TKSHPPIP+++S++A
Sbjct: 199 VILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKSHPPIPDNISSDA 258

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMVGSGLNSNDLA 360
            DFLLKCLQ+EPN RPTAS+LL+HPFV+ + ++  +    S +++    + S L +    
Sbjct: 259 NDFLLKCLQQEPNLRPTASELLKHPFVTGKQKESASKDLTSFMDNSCSPLPSELTN---- 318

Query: 361 NSTIQRSTCVGLKDICEMGSLRCS------SVYSGNYTSQSNWG-SSNFEEDMCQIDDKD 420
            ++ Q ST   + DIC +GSL C+      S+ + +   +SN G   + + DMC IDD++
Sbjct: 319 ITSYQTSTSDDVGDICNLGSLTCTLAFPEKSIQNNSLCLKSNNGYDDDDDNDMCLIDDEN 378

Query: 421 LLVSPSIKYKSIISSSDLNKSFNPMCEPTDDDWACKLDGSSELGGNLMDLSGIQTSQETT 480
            L        S+ +++D  KS + M E   D   CK D +S  G         +T  + +
Sbjct: 379 FLTYNGETGPSLDNNTDAKKSCDTMSE-ISDILKCKFDENSGNG---------ETETKVS 438

Query: 481 CKENDFTFPCGQSAVEDDEEVTESKIRAFLDEKAIELKKMQTPLYEEFRSTINAANALGA 540
            + +        S  ED+ E+TESKI+AFLD+KA ELKK+QTPLYEEF + +   + +  
Sbjct: 439 MEVDH------PSYSEDENELTESKIKAFLDDKAAELKKLQTPLYEEFYNGMITCSPICM 498

Query: 541 VGTENS----KSVSNFLKLPPKCGSPSKLR-GKRLSSIDASNCSSRQSRIKQASILEDRA 600
               N+    ++   FLKLPPK  SPS+   G+  S    + C S+      +S      
Sbjct: 499 ESNINNNKREEAPRGFLKLPPKSRSPSQGHIGRSPSRATDAACCSKSPESGNSS------ 558

Query: 601 LQEIQSPQLGEWNKLLHTQPESSTPSFSERQRKWKEELEEELKRKREMMRR 640
                +P+    +     +  S + + SE +RKWKEEL++EL+RKR  + R
Sbjct: 559 ----GAPKNSNASAGAEQESNSQSVALSEIERKWKEELDQELERKRREITR 579

BLAST of MC07g0705 vs. TAIR 10
Match: AT1G53570.1 (mitogen-activated protein kinase kinase kinase 3 )

HSP 1 Score: 289.7 bits (740), Expect = 5.9e-78
Identity = 136/264 (51.52%), Postives = 191/264 (72.35%), Query Frame = 0

Query: 65  WRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVKLLKN 124
           W++G+ +G G FG+VY+G N + G++ A+K+V + ++  + ++    +++L +E+ LL  
Sbjct: 214 WKKGKFLGSGTFGQVYLGFNSEKGKMCAIKEVKVISDDQTSKEC---LKQLNQEINLLNQ 273

Query: 125 LSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLGLEYL 184
           L HPNIV+Y G+   E++L++ LE+V GGSI  LL  +GSF E VI+ YT+Q+L GL YL
Sbjct: 274 LCHPNIVQYYGSELSEETLSVYLEYVSGGSIHKLLKDYGSFTEPVIQNYTRQILAGLAYL 333

Query: 185 HKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPEVIL- 244
           H    +HRDIKGANILVD  G IKLADFG +K V   +T+    S KG+PYWMAPEV++ 
Sbjct: 334 HGRNTVHRDIKGANILVDPNGEIKLADFGMAKHVTAFSTM---LSFKGSPYWMAPEVVMS 393

Query: 245 QTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEAKDF 304
           Q G++   DIWS+GCT++EMAT KPPWS Q++ VAA+F IGN+K  P IP+HLS +AK+F
Sbjct: 394 QNGYTHAVDIWSLGCTILEMATSKPPWS-QFEGVAAIFKIGNSKDTPEIPDHLSNDAKNF 453

Query: 305 LLKCLQEEPNSRPTASDLLQHPFV 328
           +  CLQ  P  RPTAS LL+HPF+
Sbjct: 454 IRLCLQRNPTVRPTASQLLEHPFL 470

BLAST of MC07g0705 vs. TAIR 10
Match: AT1G53570.2 (mitogen-activated protein kinase kinase kinase 3 )

HSP 1 Score: 289.7 bits (740), Expect = 5.9e-78
Identity = 136/264 (51.52%), Postives = 191/264 (72.35%), Query Frame = 0

Query: 65  WRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVKLLKN 124
           W++G+ +G G FG+VY+G N + G++ A+K+V + ++  + ++    +++L +E+ LL  
Sbjct: 214 WKKGKFLGSGTFGQVYLGFNSEKGKMCAIKEVKVISDDQTSKEC---LKQLNQEINLLNQ 273

Query: 125 LSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLGLEYL 184
           L HPNIV+Y G+   E++L++ LE+V GGSI  LL  +GSF E VI+ YT+Q+L GL YL
Sbjct: 274 LCHPNIVQYYGSELSEETLSVYLEYVSGGSIHKLLKDYGSFTEPVIQNYTRQILAGLAYL 333

Query: 185 HKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPEVIL- 244
           H    +HRDIKGANILVD  G IKLADFG +K V   +T+    S KG+PYWMAPEV++ 
Sbjct: 334 HGRNTVHRDIKGANILVDPNGEIKLADFGMAKHVTAFSTM---LSFKGSPYWMAPEVVMS 393

Query: 245 QTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEAKDF 304
           Q G++   DIWS+GCT++EMAT KPPWS Q++ VAA+F IGN+K  P IP+HLS +AK+F
Sbjct: 394 QNGYTHAVDIWSLGCTILEMATSKPPWS-QFEGVAAIFKIGNSKDTPEIPDHLSNDAKNF 453

Query: 305 LLKCLQEEPNSRPTASDLLQHPFV 328
           +  CLQ  P  RPTAS LL+HPF+
Sbjct: 454 IRLCLQRNPTVRPTASQLLEHPFL 470