MC06g_new0498 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g_new0498
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	MC06: 22947217 .. 22951962 (+)
RNA-Seq Expression	MC06g_new0498
Synteny	MC06g_new0498

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTACCGAAGGTCAAAAGTTTGCTCCTCAAAAGCAACCAACGCAGCCGGGGAAAGAGTACATCATGGACCCACCTCCTCAGTTCTCAAGCTTTCAATACAAGCCCTCAAATAAGCTTCTCGGCAAGGTATTAAATTTTCATTGATACATGTCTACATTTTTATATGTTTATGATATCGATAGGGTGAATCGGTGTCTATATTTTCATGTGTTCGTGAGTTCGACACCGACATAAGGATGAATCGATATTTTTTAATATCGTAAATAAAAAATCGATAAAATATATAAAAAAAAAGTCAAAATAATATCCAACATAACATCACTAATATGGAAATATTAATAATTTTTAATTTATTTAAAAGATTAAAAATTGTAAAATAATTATGTTAATTGAAAATTTTGTTTTTGCAAGATTGTTAATCTATTTAAAAGTTTAAAATACTTACTTGTTAATTGAATACGATTTTGTTTTTGCAATCACTTGTTAATGGAATATTCGTTACAAATTATTTAAAAATTCTATTTGACATCGATGCCATATTTAAAACCTTGTTTCTCGACAAGGTGGCATTGGTGACAGGTGGCGACTCGGGGATCGGGCGAGCCGTGTGCCACTGCTTCGCACTCGAGGGCGCCACTGTGGCATTCACCTACGTGAAGAAAGAGGAGGAGAAAGATGCTCAAGATATGCTTCAGATGCTGCTGGAGTCTCAGGCCGCCAACGCCAAGCGGCCGATTGCGATTGCCGTCGATCTGGGGTTCGACGATAATTGCAGGAGGAGGGTGGAGGAGGTGGTGGCCGCTTACGGCCGGATTGATATTTTGGTAAACACCGCTGCAGAGCCGCACAAGGCGGAGTCGGTGGAGGAGATTGATGAGCAGAGGCTTGAGAGAGTTTTTAGAACCAACATATTCTCACAGTTCTTCATGGCCAGGTAGCATTTCAAGCTTTAATACTTTAGTCTATAAATTTTCTGCTTTATTTATAGCTTGGTTACTTAATATTTAAAAATAATTATTTACTTTGAACACGTATATTTACATAGCCGCTTCCTACAGAACCTAATAGATTAGATTAATAGAATGGTACACGAATATTAGTTGTGACAATAATAAAAACTCAATAGTATCTCTTGATGTTCAAAAAATATCATAAGACTTAAATATAATATAGACTATAGAATAGTTAAATAAAATAATCTAAAATCCCATAATAAAAATGTACATCATATCTAGCTAGATAGGGAACAAGAAAGAGTAAGACAACTTGTGATTGTAGAAATTAATTTTTTTTAAAATCTAATAATTAGATAAAAACTTTGAAAAATATAGTTTCAGAGTTATAAACTCATTTTATTTTTTACTAAAATTAAAATAATATGAGTAAAAGATGAAATACAAAATTTTATAAATTTTATAATAGGTAAAATAATTGTTATACAAAATTTATAACATATATATTAATTAATCAATTATATTTTAGATTAAATTAGAATTATATTAATTTTTAAAATTGAAATTCAATTTCTAAAATATGTTACTAGAAACCTAATTGGTTAATTTTATTTTAGATTAAATTAGAATTATATTGAAATTCAGTTTCTAAATTATGTTACTAGACACCTAATTGATTAATTTTATTTTAGATTAAATTAGAATTATATTAATTTTTAAAGTTGAAATTTAATTTCTAAAATATGTTACTAAATACTCATCTGAAACCACGGACAGTAAACTTTTCATTGGATCTTTAATAATTTTCAAATTATAATCAGCAAAAGCTAGGTATGTTCATGGGCCCGGCGGCGTCCCCATCCCTCCCACCATTTATCCCCACGAGAAATTCATCCCTTTATTCTTTTTACTTCTAAAATTATTTCTCAACATTTTTTCCAAGGAAATTTATAATTAATTAATTAATTTTCAATAATAAATATTTTATTTACGTGATCTACGTATTTGAGATATAATCTTTTTAGTATCTATCTAAGTATAGAAATAATAAAAAGATTATTTTAGTATATGCATATTTTAAAAAAGTGCGGGGCAGGACGGGCAATGGGTATCGACGGGAACGGGGATGGAGATGCATTTCCCACCCGTCACAGAGAAAAAATATTTTTCCACAGAATTTAAATGGAGATTTTCAGTCTCATTTAGGTGGATCTTCACAGGATGGAATCCCATGGAAAGAATGAACTTCTCTATCAAATCAAATCTGTCGTAGTGAATGATCTATTAGTTTATAACAATATTAGACACCGGTTGTTTTCAAATTTCAAATTACAAAAATGTGTCGTAGTGAATGATCTATTAGTTTATAACAATATTAGACACCGGTTGTTTTCAAATTTCAAATTACAAAAATGTGTCGTAGTGAATGATCTATTAGTTTATAACAATATTAGACACCGGTTGTTTTCAAATTTTAAATTAAAAAATGTGTCGTAGTGAATGATCTATTAGTTTATAACAATATTAGACACCGGTTGTTTTCAAATTTCAAATTAAATCAAACCAGCAATAGAATCTCTTGTTTTGAAATTACAATCAAACGTATCGTAGTAAATGATCCATACGTGACAATACAAAAATTACGCCAGTCCATTGAGATATGGCATGGCTACAAGTGAATATTTGCGACAAGAAATGAATCCTAATTTTAGGATGACTGACCCTTTTAATCCAGTCCATATAATGTCTTTTTCGGGAGCTCGAGGGGGCGGATGCTCCTGTAGGTAGGAGCAACTTCACTTTCGGTGTTCGGCGCCGCTTTCTAAAATGATGAATATGAAGTCACCGAGTTAGTGGCTGACTGGGCTTTTTCCTAGATCAAAGCATATCAGCTCGCCTTCTTGTTATCCAAAAGTCGGTCGAATCGGGCGGGCCCCCGCCCCGGAACGATGATCCATATGTGACAATACAATATTAGGCACGCGCTGAAGCACATGAAGGAAGGCAGCTCGATCATCAACACAACATCGGTAGTCGCCTACAAGGGCAGCCCACAGCTCCTCGACTACACAGCGACAAAAGGCGCGATCGTTGCCTTCACCAGAGGTCTGGCCCTTGAGCTAGTGAGTAGAGGGATACGAGTGAATGGAGTTGCACCAGGTCCGATATGGACGCCATTGATCCCGGCATCGGTCGATGAAGAAGAAGTCAGAAGGTTCGGGTCTCATGTTCCGATGAAACGAGCTGGGCAGCCCGTGGAGGTTGCTCCATCGTATGTGTTCTTGGCCTGCAACCACTGTTCTTCCTACTTCACTGGTCAAATCCTCCATCCCAACGGTAAGTTTCCATGCAAACCAACAAAACATAAATTTTTTAACTTTATTTTACTCCATAAATTTTTATATTTATATATTTTTTTAATTTAATCTAGATTATTTAAATAGTTTTAATATAAGAAATTTTATTCCATAAACTTTTATATTTATATACTTCTTTTTATTTTTTTTAGGTAATATTACTTCTTTTAACTTAGTCTTAGACTATTTAAAAAGTTTTAATTAATATAGTGTTCCTAATGGAATAGGTAACAATGAATAAGCCACTAGCCACGATGAGTATAGTGTATATAATTATTATAAATTATAATAAGATATGTACATAAATATACTTATCATGATTCTAATCAATCATGATAGATTATTTGAATCATGCAATTTTTGGATATCAAACGGACCTCCCCAGAAGGTAATTTTAATGCCTTGATAGCTGGAAGTTCTCCAAAAGTATGAAAAGCTGGAGGACTTTGTTTTCTTATATGTTCTAGAATGATTTCCTATTTTTTTAGTTCAACACGTGGGTGGGACAATCCCACAACCTCTTAGTTAGAGATATATTTTAGTTCAAATGGGGTGGGATTCGAACTCACAACCTCTTGATTAGAGATATATGTCAATTACCGTTAAACTATGCTAGCTTTTGAAAGATTTACATTTTGTTTAACCATGATACATTTTGTTTAACCATGATACATTTTGTTTAACCATGATAGATCACACATATTTACACCGCCATGATAGATCACACATATTTACACCGGTTAATATTGAAATTGGTTTATCATGAGATTACACAACCTATTATTTAATTTTTAATCTCTATTCTAAATTTTTAAGTAGATAATTTCTTTTCTCCTTATCTTTTTTCTTTTTACCAAATTTAGTTAGTATTATGTCACGTGACTCAAATTTTAACATTTTTTTAGTGTAAATTAGAAAATCTTAAAATATAAAGAGATTAAATTGATATTTTTAAATAATAAAAACTAATTTGAAAAAAATACTTTAATATTAGGACTAAATCCCTAATAATAATATTTAGTTTGAAACGTATTTAAAATTATTTTTATGTTATTGAATGATTTTCATACTTATTTTTTTAATTATTATTTAGTGTACGTAGTCTCCTTAATTGGTTTTTGCTTTTATATTATTTGTCCAAGAACTTAAAATGTATTTCTTGGAAAGGTTAAAATAAAACAGAGATTTTAGGCTCTTCTAGGTTTCTTTTAATATATATATATATATACTCGCAAAAATTCATTTGTTGGGATTTTAAAAAACAAAAAGATTTGTTTAATTTTTTTAAAGCAAGTTTAATAAGAAATAAGGTTAAAAAAATAAATTATTAATAAATAGATCTAGTTTTTTTAATCATTTGATGATGCCTTCATTTCTTGAATATGATTTAATTTGTGTGTGAATATGCAGGTGGGACTATTGTGAATGGGTAA

mRNA sequence

ATGGCTACCGAAGGTCAAAAGTTTGCTCCTCAAAAGCAACCAACGCAGCCGGGGAAAGAGTACATCATGGACCCACCTCCTCAGTTCTCAAGCTTTCAATACAAGCCCTCAAATAAGCTTCTCGGCAAGGTGGCATTGGTGACAGGTGGCGACTCGGGGATCGGGCGAGCCGTGTGCCACTGCTTCGCACTCGAGGGCGCCACTGTGGCATTCACCTACGTGAAGAAAGAGGAGGAGAAAGATGCTCAAGATATGCTTCAGATGCTGCTGGAGTCTCAGGCCGCCAACGCCAAGCGGCCGATTGCGATTGCCGTCGATCTGGGGTTCGACGATAATTGCAGGAGGAGGGTGGAGGAGGTGGTGGCCGCTTACGGCCGGATTGATATTTTGGTAAACACCGCTGCAGAGCCGCACAAGGCGGAGTCGGTGGAGGAGATTGATGAGCAGAGGCTTGAGAGAGTTTTTAGAACCAACATATTCTCACAGTTCTTCATGGCCAGGCACGCGCTGAAGCACATGAAGGAAGGCAGCTCGATCATCAACACAACATCGGTAGTCGCCTACAAGGGCAGCCCACAGCTCCTCGACTACACAGCGACAAAAGGCGCGATCGTTGCCTTCACCAGAGGTCTGGCCCTTGAGCTAGTGAGTAGAGGGATACGAGTGAATGGAGTTGCACCAGGTCCGATATGGACGCCATTGATCCCGGCATCGGTCGATGAAGAAGAAGTCAGAAGGTTCGGGTCTCATGTTCCGATGAAACGAGCTGGGCAGCCCGTGGAGGTTGCTCCATCGTATGTGTTCTTGGCCTGCAACCACTGTTCTTCCTACTTCACTGGTCAAATCCTCCATCCCAACGGTGGGACTATTGTGAATGGGTAA

Coding sequence (CDS)

ATGGCTACCGAAGGTCAAAAGTTTGCTCCTCAAAAGCAACCAACGCAGCCGGGGAAAGAGTACATCATGGACCCACCTCCTCAGTTCTCAAGCTTTCAATACAAGCCCTCAAATAAGCTTCTCGGCAAGGTGGCATTGGTGACAGGTGGCGACTCGGGGATCGGGCGAGCCGTGTGCCACTGCTTCGCACTCGAGGGCGCCACTGTGGCATTCACCTACGTGAAGAAAGAGGAGGAGAAAGATGCTCAAGATATGCTTCAGATGCTGCTGGAGTCTCAGGCCGCCAACGCCAAGCGGCCGATTGCGATTGCCGTCGATCTGGGGTTCGACGATAATTGCAGGAGGAGGGTGGAGGAGGTGGTGGCCGCTTACGGCCGGATTGATATTTTGGTAAACACCGCTGCAGAGCCGCACAAGGCGGAGTCGGTGGAGGAGATTGATGAGCAGAGGCTTGAGAGAGTTTTTAGAACCAACATATTCTCACAGTTCTTCATGGCCAGGCACGCGCTGAAGCACATGAAGGAAGGCAGCTCGATCATCAACACAACATCGGTAGTCGCCTACAAGGGCAGCCCACAGCTCCTCGACTACACAGCGACAAAAGGCGCGATCGTTGCCTTCACCAGAGGTCTGGCCCTTGAGCTAGTGAGTAGAGGGATACGAGTGAATGGAGTTGCACCAGGTCCGATATGGACGCCATTGATCCCGGCATCGGTCGATGAAGAAGAAGTCAGAAGGTTCGGGTCTCATGTTCCGATGAAACGAGCTGGGCAGCCCGTGGAGGTTGCTCCATCGTATGTGTTCTTGGCCTGCAACCACTGTTCTTCCTACTTCACTGGTCAAATCCTCCATCCCAACGGTGGGACTATTGTGAATGGGTAA

Protein sequence

MATEGQKFAPQKQPTQPGKEYIMDPPPQFSSFQYKPSNKLLGKVALVTGGDSGIGRAVCHCFALEGATVAFTYVKKEEEKDAQDMLQMLLESQAANAKRPIAIAVDLGFDDNCRRRVEEVVAAYGRIDILVNTAAEPHKAESVEEIDEQRLERVFRTNIFSQFFMARHALKHMKEGSSIINTTSVVAYKGSPQLLDYTATKGAIVAFTRGLALELVSRGIRVNGVAPGPIWTPLIPASVDEEEVRRFGSHVPMKRAGQPVEVAPSYVFLACNHCSSYFTGQILHPNGGTIVNG

Homology

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BLAST of MC06g_new0498 vs. ExPASy Swiss-Prot
Match: Q9FZ42 (NADPH-dependent aldehyde reductase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ChlADR1 PE=1 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 1.6e-123
Identity = 214/282 (75.89%), Postives = 248/282 (87.94%), Query Frame = 0


Query: 11  QKQPTQPGKEYIMDPPPQFSSFQYKPSNKLLGKVALVTGGDSGIGRAVCHCFALEGATVA 70
           QKQ  QPGKE++M+  PQFSS  Y+PSNKL GKVAL+TGGDSGIGRAV +CFA EGATVA
Sbjct: 6   QKQHAQPGKEHVMESSPQFSSSDYQPSNKLRGKVALITGGDSGIGRAVGYCFASEGATVA 65

Query: 71  FTYVKKEEEKDAQDMLQMLLESQAANAKRPIAIAVDLGFDDNCRRRVEEVVAAYGRIDIL 130
           FTYVK +EEKDAQ+ LQML E + +++K PIAI  DLGFD+NC+R V+EVV A+GRID+L
Sbjct: 66  FTYVKGQEEKDAQETLQMLKEVKTSDSKEPIAIPTDLGFDENCKRVVDEVVNAFGRIDVL 125

Query: 131 VNTAAEPHKAESVEEIDEQRLERVFRTNIFSQFFMARHALKHMKEGSSIINTTSVVAYKG 190
           +N AAE +++ ++EEIDE RLERVFRTNIFS FF+ RHALKHMKEGSSIINTTSV AYKG
Sbjct: 126 INNAAEQYESSTIEEIDEPRLERVFRTNIFSYFFLTRHALKHMKEGSSIINTTSVNAYKG 185

Query: 191 SPQLLDYTATKGAIVAFTRGLALELVSRGIRVNGVAPGPIWTPLIPASVDEEEVRRFGSH 250
           +  LLDYTATKGAIVAFTRGLAL+L  +GIRVNGVAPGPIWTPLIPAS +EE+++ FGS 
Sbjct: 186 NASLLDYTATKGAIVAFTRGLALQLAEKGIRVNGVAPGPIWTPLIPASFNEEKIKNFGSE 245

Query: 251 VPMKRAGQPVEVAPSYVFLACNHCSSYFTGQILHPNGGTIVN 293
           VPMKRAGQP+EVAPSYVFLACNHCSSYFTGQ+LHPNGG +VN
Sbjct: 246 VPMKRAGQPIEVAPSYVFLACNHCSSYFTGQVLHPNGGAVVN 287

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BLAST of MC06g_new0498 vs. ExPASy Swiss-Prot
Match: Q9MA93 (Glucose and ribitol dehydrogenase homolog 2 OS=Arabidopsis thaliana OX=3702 GN=At3g05260 PE=2 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 3.3e-121
Identity = 211/285 (74.04%), Postives = 247/285 (86.67%), Query Frame = 0


Query: 8   FAPQKQPTQPGKEYIMDPPPQFSSFQYKPSNKLLGKVALVTGGDSGIGRAVCHCFALEGA 67
           F PQKQ TQPG +++M+P P+FSS  YKPSNKL GKVALVTGGDSGIG+AVCHC+ALEGA
Sbjct: 5   FPPQKQETQPGIQHVMEPTPEFSSSNYKPSNKLHGKVALVTGGDSGIGKAVCHCYALEGA 64

Query: 68  TVAFTYVKKEEEKDAQDMLQMLLESQAANAKRPIAIAVDLGFDDNCRRRVEEVVAAYGRI 127
           +VAFTYVK  E+KDA++ L++L E +   AK PI IA DLGF++NC+R VEEVV ++GRI
Sbjct: 65  SVAFTYVKGREDKDAEETLRLLHEVKTREAKEPIMIATDLGFEENCKRVVEEVVNSFGRI 124

Query: 128 DILVNTAAEPHKAESVEEIDEQRLERVFRTNIFSQFFMARHALKHMKEGSSIINTTSVVA 187
           D+LVN AAE H+  S+E+IDE RLERVFRTNIFSQFF+ ++ALKHMKEGSSIINTTSVVA
Sbjct: 125 DVLVNCAAEQHEV-SIEDIDEARLERVFRTNIFSQFFLVKYALKHMKEGSSIINTTSVVA 184

Query: 188 YKGSPQLLDYTATKGAIVAFTRGLALELVSRGIRVNGVAPGPIWTPLIPASVDEEEVRRF 247
           Y G+  LL+YTATKGAIV+FTRGLAL+L  +GIRVNGVAPGP+WTPLIPAS  EE +++F
Sbjct: 185 YAGNSSLLEYTATKGAIVSFTRGLALQLAPKGIRVNGVAPGPVWTPLIPASFSEEAIKQF 244

Query: 248 GSHVPMKRAGQPVEVAPSYVFLACNHCSSYFTGQILHPNGGTIVN 293
           GS  PMKRA QPVEVAPSYVFLACNHCSSY+TGQILHPNGG IVN
Sbjct: 245 GSETPMKRAAQPVEVAPSYVFLACNHCSSYYTGQILHPNGGLIVN 288

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BLAST of MC06g_new0498 vs. ExPASy Swiss-Prot
Match: Q5KTS5 (Glucose and ribitol dehydrogenase OS=Daucus carota OX=4039 GN=CAISE5 PE=2 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 1.2e-118
Identity = 211/294 (71.77%), Postives = 260/294 (88.44%), Query Frame = 0


Query: 1   MATEGQKFAPQKQPTQPGKEYIMDPPPQFSSFQYKPSNKLLGKVALVTGGDSGIGRAVCH 60
           MA+ GQ F PQKQ +QPGKE++MDP PQ +S  YKP+NKL GKVALVTGGDSGIGR+VC+
Sbjct: 1   MASGGQ-FPPQKQESQPGKEHLMDPSPQHASPHYKPANKLQGKVALVTGGDSGIGRSVCY 60

Query: 61  CFALEGATVAFTYVKKEEEKDAQDMLQMLLESQAANAKRPIAIAVDLGFDDNCRRRVEEV 120
            FALEGATVAFT+VK  E+KDA + L++L ++++++AK PIAIA DLGFDDNC++ V++V
Sbjct: 61  HFALEGATVAFTFVKGHEDKDANETLELLRKAKSSDAKDPIAIAADLGFDDNCKKVVDQV 120

Query: 121 VAAYGRIDILVNTAAEPHKAESVEEIDEQRLERVFRTNIFSQFFMARHALKHMKEGSSII 180
           V A+G ID+LVN AAE +KA +VE+IDE+RLERVFRTNIF+ FFMARHALKHM+EGS+II
Sbjct: 121 VNAFGSIDVLVNNAAEQYKASTVEDIDEERLERVFRTNIFAYFFMARHALKHMREGSTII 180

Query: 181 NTTSVVAYKGSPQLLDYTATKGAIVAFTRGLALELVSRGIRVNGVAPGPIWTPLIPASVD 240
           NTTS+ AYKG+ +LLDYTATKGAIVAFTRGL+L+L+S+GIRVNGVAPGP+WTPLIP+S D
Sbjct: 181 NTTSINAYKGNAKLLDYTATKGAIVAFTRGLSLQLISKGIRVNGVAPGPVWTPLIPSSFD 240

Query: 241 EEEVRRFGSHVPMKRAGQPVEVAPSYVFLA-CNHCSSYFTGQILHPNGGTIVNG 294
           EEEV++FGS VPMKRAGQP E+A +YVFLA C+  SSY++GQ+LHPNGG IVNG
Sbjct: 241 EEEVKQFGSEVPMKRAGQPYEIATAYVFLASCD--SSYYSGQVLHPNGGAIVNG 291

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BLAST of MC06g_new0498 vs. ExPASy Swiss-Prot
Match: Q75KH3 (Glucose and ribitol dehydrogenase homolog OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0140800 PE=2 SV=2)

HSP 1 Score: 372.1 bits (954), Expect = 5.8e-102
Identity = 197/298 (66.11%), Postives = 232/298 (77.85%), Query Frame = 0


Query: 6   QKFAPQKQPTQPGKEYIMDPPPQFSSFQYKPSNKLLGKVALVTGGDSGIGRAVCHCFALE 65
           Q+F PQ Q TQPGKE+ MDP P+     YKP+NKL  KVA+VTGGDSGIGRAVC CFALE
Sbjct: 4   QQFPPQNQETQPGKEHAMDPRPEAIIQSYKPANKLKDKVAIVTGGDSGIGRAVCLCFALE 63

Query: 66  GATVAFTYVKKEEEKDAQDMLQMLLESQA-ANAKRPIAIAVDLGFDDNCRRRVEEVVAAY 125
           GATVAFTYVK +EEKDA++ L+ L + +A   AK P+AI  DLG+DDNCR+ V+EV  AY
Sbjct: 64  GATVAFTYVKGQEEKDAEETLRALRDIRARTGAKDPMAIPADLGYDDNCRKVVDEVAGAY 123

Query: 126 -GRIDILVNTAAEPHKAESVEEIDEQRLERVFRTNIFSQFFMARHALKHMKE-------- 185
            G IDILVN AAE ++  S+ +I E  LERVFRTNIFS FFM++HA+K M++        
Sbjct: 124 GGAIDILVNNAAEQYERPSITDITEDDLERVFRTNIFSYFFMSKHAVKRMRDRRGGAGAG 183

Query: 186 GSSIINTTSVVAYKGSPQLLDYTATKGAIVAFTRGLALELVSRGIRVNGVAPGPIWTPLI 245
           G SIINT+S+ AYKG+  LLDYTATKGAIVAFTR LAL+L   GIRVNGVAPGPIWTPLI
Sbjct: 184 GCSIINTSSINAYKGNKTLLDYTATKGAIVAFTRALALQLAEEGIRVNGVAPGPIWTPLI 243

Query: 246 PASVDEEEVRRFGSHVPMKRAGQPVEVAPSYVFLACNHCSSYFTGQILHPNGGTIVNG 294
           PAS  EE+VR+FGS VPM RAGQP EVAPS+VFLA +  +SY +GQ+LH NGG IVNG
Sbjct: 244 PASFAEEKVRQFGSQVPMGRAGQPSEVAPSFVFLASDD-ASYMSGQMLHVNGGVIVNG 300

[Close]

BLAST of MC06g_new0498 vs. ExPASy Swiss-Prot
Match: P40397 (Uncharacterized oxidoreductase YhxC OS=Bacillus subtilis (strain 168) OX=224308 GN=yhxC PE=3 SV=2)

HSP 1 Score: 280.0 bits (715), Expect = 3.0e-74
Identity = 157/294 (53.40%), Postives = 198/294 (67.35%), Query Frame = 0


Query: 1   MATEGQK-FAPQKQPTQPGKEYIMDPPPQFSSFQYKPSNKLLGKVALVTGGDSGIGRAVC 60
           MA + +K   PQ Q  QPG EY+MDP P F   + K + KL GK A++TGGDSGIGRAV 
Sbjct: 1   MANQKKKTLPPQHQNQQPGFEYLMDPRPVFD--KPKKAKKLEGKTAIITGGDSGIGRAVS 60

Query: 61  HCFALEGATVAFTYVKKEEEKDAQDMLQMLLESQAANAKRPIAIAVDLGFDDNCRRRVEE 120
             FA EGA V   Y+   E +DA++  Q  +E +       + IA D+G +  C   V +
Sbjct: 61  VLFAKEGANVVIVYL--NEHQDAEETKQ-YVEKEGVKC---LLIAGDVGDEAFCNDVVGQ 120

Query: 121 VVAAYGRIDILVNTAAEPHKAESVEEIDEQRLERVFRTNIFSQFFMARHALKHMKEGSSI 180
               +  IDILVN AAE H   S+E+I   +L R F+TNIFS F++ +  L H+K+GSSI
Sbjct: 121 ASQVFPSIDILVNNAAEQHVQPSIEKITSHQLIRTFQTNIFSMFYLTKAVLPHLKKGSSI 180

Query: 181 INTTSVVAYKGSPQLLDYTATKGAIVAFTRGLALELVSRGIRVNGVAPGPIWTPLIPASV 240
           INT S+ AYKG+  L+DY+ATKGAIV FTR L+  LV +GIRVN VAPGPIWTPLIPAS 
Sbjct: 181 INTASITAYKGNKTLIDYSATKGAIVTFTRSLSQSLVQQGIRVNAVAPGPIWTPLIPASF 240

Query: 241 DEEEVRRFGSHVPMKRAGQPVEVAPSYVFLACNHCSSYFTGQILHPNGGTIVNG 294
             ++V  FGS VPM+R GQPVEVAPSY++LA +  S+Y TGQ +H NGGTIVNG
Sbjct: 241 AAKDVEVFGSDVPMERPGQPVEVAPSYLYLASDD-STYVTGQTIHVNGGTIVNG 285

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BLAST of MC06g_new0498 vs. NCBI nr
Match: XP_022156046.1 (NADPH-dependent aldehyde reductase 1, chloroplastic [Momordica charantia])

HSP 1 Score: 587 bits (1512), Expect = 2.72e-211
Identity = 293/293 (100.00%), Postives = 293/293 (100.00%), Query Frame = 0


Query: 1   MATEGQKFAPQKQPTQPGKEYIMDPPPQFSSFQYKPSNKLLGKVALVTGGDSGIGRAVCH 60
           MATEGQKFAPQKQPTQPGKEYIMDPPPQFSSFQYKPSNKLLGKVALVTGGDSGIGRAVCH
Sbjct: 1   MATEGQKFAPQKQPTQPGKEYIMDPPPQFSSFQYKPSNKLLGKVALVTGGDSGIGRAVCH 60

Query: 61  CFALEGATVAFTYVKKEEEKDAQDMLQMLLESQAANAKRPIAIAVDLGFDDNCRRRVEEV 120
           CFALEGATVAFTYVKKEEEKDAQDMLQMLLESQAANAKRPIAIAVDLGFDDNCRRRVEEV
Sbjct: 61  CFALEGATVAFTYVKKEEEKDAQDMLQMLLESQAANAKRPIAIAVDLGFDDNCRRRVEEV 120

Query: 121 VAAYGRIDILVNTAAEPHKAESVEEIDEQRLERVFRTNIFSQFFMARHALKHMKEGSSII 180
           VAAYGRIDILVNTAAEPHKAESVEEIDEQRLERVFRTNIFSQFFMARHALKHMKEGSSII
Sbjct: 121 VAAYGRIDILVNTAAEPHKAESVEEIDEQRLERVFRTNIFSQFFMARHALKHMKEGSSII 180

Query: 181 NTTSVVAYKGSPQLLDYTATKGAIVAFTRGLALELVSRGIRVNGVAPGPIWTPLIPASVD 240
           NTTSVVAYKGSPQLLDYTATKGAIVAFTRGLALELVSRGIRVNGVAPGPIWTPLIPASVD
Sbjct: 181 NTTSVVAYKGSPQLLDYTATKGAIVAFTRGLALELVSRGIRVNGVAPGPIWTPLIPASVD 240

Query: 241 EEEVRRFGSHVPMKRAGQPVEVAPSYVFLACNHCSSYFTGQILHPNGGTIVNG 293
           EEEVRRFGSHVPMKRAGQPVEVAPSYVFLACNHCSSYFTGQILHPNGGTIVNG
Sbjct: 241 EEEVRRFGSHVPMKRAGQPVEVAPSYVFLACNHCSSYFTGQILHPNGGTIVNG 293

[Close]

BLAST of MC06g_new0498 vs. NCBI nr
Match: XP_023530263.1 (NADPH-dependent aldehyde reductase 1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 517 bits (1332), Expect = 7.42e-184
Identity = 249/293 (84.98%), Postives = 271/293 (92.49%), Query Frame = 0


Query: 1   MATEGQKFAPQKQPTQPGKEYIMDPPPQFSSFQYKPSNKLLGKVALVTGGDSGIGRAVCH 60
           MA+EG KF PQKQPTQPGKE++MDPPPQF+SFQYKPSNKLLG+VALVTGGDSGIGRAVCH
Sbjct: 1   MASEGHKFPPQKQPTQPGKEHVMDPPPQFTSFQYKPSNKLLGRVALVTGGDSGIGRAVCH 60

Query: 61  CFALEGATVAFTYVKKEEEKDAQDMLQMLLESQAANAKRPIAIAVDLGFDDNCRRRVEEV 120
           CFALEGATVAFTYVK +EEKDA+DML +L E QAA+AK PIA+  DLGFD+NC+R ++EV
Sbjct: 61  CFALEGATVAFTYVKNQEEKDARDMLGILRECQAADAKPPIAVGTDLGFDENCKRAIDEV 120

Query: 121 VAAYGRIDILVNTAAEPHKAESVEEIDEQRLERVFRTNIFSQFFMARHALKHMKEGSSII 180
           VAAYGRID+LVN AAE HK +SVEEIDEQRLERVFRTNIFSQFFM +HALKHMKEGSSII
Sbjct: 121 VAAYGRIDVLVNAAAEQHKTQSVEEIDEQRLERVFRTNIFSQFFMVKHALKHMKEGSSII 180

Query: 181 NTTSVVAYKGSPQLLDYTATKGAIVAFTRGLALELVSRGIRVNGVAPGPIWTPLIPASVD 240
           NTTSVVAYKG+PQLLDY ATKGAIVAFTRGLAL+L SRGIRVNGVAPGPIWTPLIPAS D
Sbjct: 181 NTTSVVAYKGNPQLLDYAATKGAIVAFTRGLALQLASRGIRVNGVAPGPIWTPLIPASFD 240

Query: 241 EEEVRRFGSHVPMKRAGQPVEVAPSYVFLACNHCSSYFTGQILHPNGGTIVNG 293
            EE+  FGS VPMKRAGQP+EV PSYVFLACNHCSSYFTGQILHPNGG+IVNG
Sbjct: 241 NEEIESFGSEVPMKRAGQPIEVGPSYVFLACNHCSSYFTGQILHPNGGSIVNG 293

[Close]

BLAST of MC06g_new0498 vs. NCBI nr
Match: KAG6588473.1 (NADPH-dependent aldehyde reductase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 516 bits (1330), Expect = 1.61e-183
Identity = 249/293 (84.98%), Postives = 271/293 (92.49%), Query Frame = 0


Query: 1   MATEGQKFAPQKQPTQPGKEYIMDPPPQFSSFQYKPSNKLLGKVALVTGGDSGIGRAVCH 60
           MA+EG KF PQKQPTQPGKE++MDPPPQF+SFQYKPSNKLLG+VALVTGGDSGIGRAVCH
Sbjct: 1   MASEGHKFPPQKQPTQPGKEHVMDPPPQFTSFQYKPSNKLLGRVALVTGGDSGIGRAVCH 60

Query: 61  CFALEGATVAFTYVKKEEEKDAQDMLQMLLESQAANAKRPIAIAVDLGFDDNCRRRVEEV 120
           CFALEGATVAFTYVK +EEKDA+DML +L E QAA+AK PIA+  DLGFD+NC+R +++V
Sbjct: 61  CFALEGATVAFTYVKNQEEKDARDMLGILRECQAADAKPPIAVGTDLGFDENCKRAIDKV 120

Query: 121 VAAYGRIDILVNTAAEPHKAESVEEIDEQRLERVFRTNIFSQFFMARHALKHMKEGSSII 180
           VAAYGRID+LVN AAE HK  SVEEIDEQRLERVFRTNIFSQFFM +HALKHMKEGSSII
Sbjct: 121 VAAYGRIDVLVNAAAEQHKTHSVEEIDEQRLERVFRTNIFSQFFMVKHALKHMKEGSSII 180

Query: 181 NTTSVVAYKGSPQLLDYTATKGAIVAFTRGLALELVSRGIRVNGVAPGPIWTPLIPASVD 240
           NTTSVVAYKGSPQLLDY ATKGAIVAFTRGLAL+L SRGIRVNGVAPGPIWTPLIPAS D
Sbjct: 181 NTTSVVAYKGSPQLLDYAATKGAIVAFTRGLALQLASRGIRVNGVAPGPIWTPLIPASFD 240

Query: 241 EEEVRRFGSHVPMKRAGQPVEVAPSYVFLACNHCSSYFTGQILHPNGGTIVNG 293
           +EE+  FGS VPMKRAGQP+EV PSYVFLACNHCSSYFTGQILHPNGG+IVNG
Sbjct: 241 KEEIESFGSEVPMKRAGQPIEVGPSYVFLACNHCSSYFTGQILHPNGGSIVNG 293

[Close]

BLAST of MC06g_new0498 vs. NCBI nr
Match: XP_022970115.1 (NADPH-dependent aldehyde reductase 1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 515 bits (1327), Expect = 4.29e-183
Identity = 248/293 (84.64%), Postives = 270/293 (92.15%), Query Frame = 0


Query: 1   MATEGQKFAPQKQPTQPGKEYIMDPPPQFSSFQYKPSNKLLGKVALVTGGDSGIGRAVCH 60
           MA+EG KF PQKQPTQPGKE++MDPPPQF+SFQYKPSNKLLG+VALVTGGDSGIGRAVCH
Sbjct: 1   MASEGHKFPPQKQPTQPGKEHVMDPPPQFTSFQYKPSNKLLGRVALVTGGDSGIGRAVCH 60

Query: 61  CFALEGATVAFTYVKKEEEKDAQDMLQMLLESQAANAKRPIAIAVDLGFDDNCRRRVEEV 120
           CFALEGATVAFTYVK +EEKDA+DML +L E QAA+AK PIA+  DLGFD+NC+R ++EV
Sbjct: 61  CFALEGATVAFTYVKNQEEKDARDMLGILRECQAADAKPPIAVGTDLGFDENCKRAIDEV 120

Query: 121 VAAYGRIDILVNTAAEPHKAESVEEIDEQRLERVFRTNIFSQFFMARHALKHMKEGSSII 180
           VAAYGRID+LVN AAE HK +SVEEIDEQRLERVFRTNIFSQFFM +HA+KHMKEGSSII
Sbjct: 121 VAAYGRIDVLVNAAAEQHKTQSVEEIDEQRLERVFRTNIFSQFFMVKHAVKHMKEGSSII 180

Query: 181 NTTSVVAYKGSPQLLDYTATKGAIVAFTRGLALELVSRGIRVNGVAPGPIWTPLIPASVD 240
           NTTSVVAYKGSPQLLDY ATKGAIVAFTRGLAL+L  RGIRVNGVAPGPIWTPLIPAS D
Sbjct: 181 NTTSVVAYKGSPQLLDYAATKGAIVAFTRGLALQLARRGIRVNGVAPGPIWTPLIPASFD 240

Query: 241 EEEVRRFGSHVPMKRAGQPVEVAPSYVFLACNHCSSYFTGQILHPNGGTIVNG 293
            EE+  FGS VPMKRAGQP+EV PSYVFLACNHCSSYFTGQILHPNGG+IVNG
Sbjct: 241 NEEIESFGSEVPMKRAGQPIEVGPSYVFLACNHCSSYFTGQILHPNGGSIVNG 293

[Close]

BLAST of MC06g_new0498 vs. NCBI nr
Match: XP_022926275.1 (NADPH-dependent aldehyde reductase 1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 512 bits (1319), Expect = 7.10e-182
Identity = 247/293 (84.30%), Postives = 269/293 (91.81%), Query Frame = 0


Query: 1   MATEGQKFAPQKQPTQPGKEYIMDPPPQFSSFQYKPSNKLLGKVALVTGGDSGIGRAVCH 60
           MA+EG KF PQKQPTQPGKE++MDPPPQF+SFQYKPSNKLLG+VALVTGGDSGIGRAVCH
Sbjct: 1   MASEGHKFPPQKQPTQPGKEHVMDPPPQFTSFQYKPSNKLLGRVALVTGGDSGIGRAVCH 60

Query: 61  CFALEGATVAFTYVKKEEEKDAQDMLQMLLESQAANAKRPIAIAVDLGFDDNCRRRVEEV 120
           CFALEGATVAFTYVK +EEKDA+DML +L E QAA+AK PIA+  DLGFD+NC++ ++EV
Sbjct: 61  CFALEGATVAFTYVKNQEEKDARDMLGILRECQAADAKPPIAVGTDLGFDENCKQAIDEV 120

Query: 121 VAAYGRIDILVNTAAEPHKAESVEEIDEQRLERVFRTNIFSQFFMARHALKHMKEGSSII 180
           VAAYGRID+LVN AAE HK  SVEEIDEQRLERVFRTNIFSQFFM +HALKHMKEGSSII
Sbjct: 121 VAAYGRIDVLVNAAAEQHKTHSVEEIDEQRLERVFRTNIFSQFFMVKHALKHMKEGSSII 180

Query: 181 NTTSVVAYKGSPQLLDYTATKGAIVAFTRGLALELVSRGIRVNGVAPGPIWTPLIPASVD 240
           NTTSVVAYKGSPQLLDY ATKGAIVAFTRGLA +L SRGIRVNGV PGPIWTPLIPAS D
Sbjct: 181 NTTSVVAYKGSPQLLDYAATKGAIVAFTRGLAHQLASRGIRVNGVVPGPIWTPLIPASFD 240

Query: 241 EEEVRRFGSHVPMKRAGQPVEVAPSYVFLACNHCSSYFTGQILHPNGGTIVNG 293
           +EE+  FGS VPMKRAGQP+EV PSYVFLACNHCSSYFTGQILHPNGG+IVNG
Sbjct: 241 KEEIESFGSEVPMKRAGQPIEVGPSYVFLACNHCSSYFTGQILHPNGGSIVNG 293

[Close]

BLAST of MC06g_new0498 vs. ExPASy TrEMBL
Match: A0A6J1DTN4 (NADPH-dependent aldehyde reductase 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111023012 PE=4 SV=1)

HSP 1 Score: 587 bits (1512), Expect = 1.32e-211
Identity = 293/293 (100.00%), Postives = 293/293 (100.00%), Query Frame = 0


Query: 1   MATEGQKFAPQKQPTQPGKEYIMDPPPQFSSFQYKPSNKLLGKVALVTGGDSGIGRAVCH 60
           MATEGQKFAPQKQPTQPGKEYIMDPPPQFSSFQYKPSNKLLGKVALVTGGDSGIGRAVCH
Sbjct: 1   MATEGQKFAPQKQPTQPGKEYIMDPPPQFSSFQYKPSNKLLGKVALVTGGDSGIGRAVCH 60

Query: 61  CFALEGATVAFTYVKKEEEKDAQDMLQMLLESQAANAKRPIAIAVDLGFDDNCRRRVEEV 120
           CFALEGATVAFTYVKKEEEKDAQDMLQMLLESQAANAKRPIAIAVDLGFDDNCRRRVEEV
Sbjct: 61  CFALEGATVAFTYVKKEEEKDAQDMLQMLLESQAANAKRPIAIAVDLGFDDNCRRRVEEV 120

Query: 121 VAAYGRIDILVNTAAEPHKAESVEEIDEQRLERVFRTNIFSQFFMARHALKHMKEGSSII 180
           VAAYGRIDILVNTAAEPHKAESVEEIDEQRLERVFRTNIFSQFFMARHALKHMKEGSSII
Sbjct: 121 VAAYGRIDILVNTAAEPHKAESVEEIDEQRLERVFRTNIFSQFFMARHALKHMKEGSSII 180

Query: 181 NTTSVVAYKGSPQLLDYTATKGAIVAFTRGLALELVSRGIRVNGVAPGPIWTPLIPASVD 240
           NTTSVVAYKGSPQLLDYTATKGAIVAFTRGLALELVSRGIRVNGVAPGPIWTPLIPASVD
Sbjct: 181 NTTSVVAYKGSPQLLDYTATKGAIVAFTRGLALELVSRGIRVNGVAPGPIWTPLIPASVD 240

Query: 241 EEEVRRFGSHVPMKRAGQPVEVAPSYVFLACNHCSSYFTGQILHPNGGTIVNG 293
           EEEVRRFGSHVPMKRAGQPVEVAPSYVFLACNHCSSYFTGQILHPNGGTIVNG
Sbjct: 241 EEEVRRFGSHVPMKRAGQPVEVAPSYVFLACNHCSSYFTGQILHPNGGTIVNG 293

[Close]

BLAST of MC06g_new0498 vs. ExPASy TrEMBL
Match: A0A6J1I1Y4 (NADPH-dependent aldehyde reductase 1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111469102 PE=4 SV=1)

HSP 1 Score: 515 bits (1327), Expect = 2.08e-183
Identity = 248/293 (84.64%), Postives = 270/293 (92.15%), Query Frame = 0


Query: 1   MATEGQKFAPQKQPTQPGKEYIMDPPPQFSSFQYKPSNKLLGKVALVTGGDSGIGRAVCH 60
           MA+EG KF PQKQPTQPGKE++MDPPPQF+SFQYKPSNKLLG+VALVTGGDSGIGRAVCH
Sbjct: 1   MASEGHKFPPQKQPTQPGKEHVMDPPPQFTSFQYKPSNKLLGRVALVTGGDSGIGRAVCH 60

Query: 61  CFALEGATVAFTYVKKEEEKDAQDMLQMLLESQAANAKRPIAIAVDLGFDDNCRRRVEEV 120
           CFALEGATVAFTYVK +EEKDA+DML +L E QAA+AK PIA+  DLGFD+NC+R ++EV
Sbjct: 61  CFALEGATVAFTYVKNQEEKDARDMLGILRECQAADAKPPIAVGTDLGFDENCKRAIDEV 120

Query: 121 VAAYGRIDILVNTAAEPHKAESVEEIDEQRLERVFRTNIFSQFFMARHALKHMKEGSSII 180
           VAAYGRID+LVN AAE HK +SVEEIDEQRLERVFRTNIFSQFFM +HA+KHMKEGSSII
Sbjct: 121 VAAYGRIDVLVNAAAEQHKTQSVEEIDEQRLERVFRTNIFSQFFMVKHAVKHMKEGSSII 180

Query: 181 NTTSVVAYKGSPQLLDYTATKGAIVAFTRGLALELVSRGIRVNGVAPGPIWTPLIPASVD 240
           NTTSVVAYKGSPQLLDY ATKGAIVAFTRGLAL+L  RGIRVNGVAPGPIWTPLIPAS D
Sbjct: 181 NTTSVVAYKGSPQLLDYAATKGAIVAFTRGLALQLARRGIRVNGVAPGPIWTPLIPASFD 240

Query: 241 EEEVRRFGSHVPMKRAGQPVEVAPSYVFLACNHCSSYFTGQILHPNGGTIVNG 293
            EE+  FGS VPMKRAGQP+EV PSYVFLACNHCSSYFTGQILHPNGG+IVNG
Sbjct: 241 NEEIESFGSEVPMKRAGQPIEVGPSYVFLACNHCSSYFTGQILHPNGGSIVNG 293

[Close]

BLAST of MC06g_new0498 vs. ExPASy TrEMBL
Match: A0A6J1EKN0 (NADPH-dependent aldehyde reductase 1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111433449 PE=4 SV=1)

HSP 1 Score: 512 bits (1319), Expect = 3.44e-182
Identity = 247/293 (84.30%), Postives = 269/293 (91.81%), Query Frame = 0


Query: 1   MATEGQKFAPQKQPTQPGKEYIMDPPPQFSSFQYKPSNKLLGKVALVTGGDSGIGRAVCH 60
           MA+EG KF PQKQPTQPGKE++MDPPPQF+SFQYKPSNKLLG+VALVTGGDSGIGRAVCH
Sbjct: 1   MASEGHKFPPQKQPTQPGKEHVMDPPPQFTSFQYKPSNKLLGRVALVTGGDSGIGRAVCH 60

Query: 61  CFALEGATVAFTYVKKEEEKDAQDMLQMLLESQAANAKRPIAIAVDLGFDDNCRRRVEEV 120
           CFALEGATVAFTYVK +EEKDA+DML +L E QAA+AK PIA+  DLGFD+NC++ ++EV
Sbjct: 61  CFALEGATVAFTYVKNQEEKDARDMLGILRECQAADAKPPIAVGTDLGFDENCKQAIDEV 120

Query: 121 VAAYGRIDILVNTAAEPHKAESVEEIDEQRLERVFRTNIFSQFFMARHALKHMKEGSSII 180
           VAAYGRID+LVN AAE HK  SVEEIDEQRLERVFRTNIFSQFFM +HALKHMKEGSSII
Sbjct: 121 VAAYGRIDVLVNAAAEQHKTHSVEEIDEQRLERVFRTNIFSQFFMVKHALKHMKEGSSII 180

Query: 181 NTTSVVAYKGSPQLLDYTATKGAIVAFTRGLALELVSRGIRVNGVAPGPIWTPLIPASVD 240
           NTTSVVAYKGSPQLLDY ATKGAIVAFTRGLA +L SRGIRVNGV PGPIWTPLIPAS D
Sbjct: 181 NTTSVVAYKGSPQLLDYAATKGAIVAFTRGLAHQLASRGIRVNGVVPGPIWTPLIPASFD 240

Query: 241 EEEVRRFGSHVPMKRAGQPVEVAPSYVFLACNHCSSYFTGQILHPNGGTIVNG 293
           +EE+  FGS VPMKRAGQP+EV PSYVFLACNHCSSYFTGQILHPNGG+IVNG
Sbjct: 241 KEEIESFGSEVPMKRAGQPIEVGPSYVFLACNHCSSYFTGQILHPNGGSIVNG 293

[Close]

BLAST of MC06g_new0498 vs. ExPASy TrEMBL
Match: A0A1S3BQQ8 (glucose and ribitol dehydrogenase homolog 1 OS=Cucumis melo OX=3656 GN=LOC103492178 PE=4 SV=1)

HSP 1 Score: 504 bits (1298), Expect = 5.44e-179
Identity = 244/293 (83.28%), Postives = 270/293 (92.15%), Query Frame = 0


Query: 1   MATEGQKFAPQKQPTQPGKEYIMDPPPQFSSFQYKPSNKLLGKVALVTGGDSGIGRAVCH 60
           MATEGQ   PQKQPTQPGKE++MDP PQF+S QYKPSNKLLGKVALVTGGDSGIGRAVCH
Sbjct: 1   MATEGQTCPPQKQPTQPGKEHVMDPSPQFTSSQYKPSNKLLGKVALVTGGDSGIGRAVCH 60

Query: 61  CFALEGATVAFTYVKKEEEKDAQDMLQMLLESQAANAKRPIAIAVDLGFDDNCRRRVEEV 120
           CFALEGATVAFTYVKK+E+KDA+D+L++L E QAA+AK+PIA+  DLGFD+ C+  V+EV
Sbjct: 61  CFALEGATVAFTYVKKQEDKDAKDVLEILRECQAADAKQPIAVGADLGFDEECKHVVDEV 120

Query: 121 VAAYGRIDILVNTAAEPHKAESVEEIDEQRLERVFRTNIFSQFFMARHALKHMKEGSSII 180
           V AYGRIDILVN+AAE HK  SVEEIDE+R+ERVFRTNIFSQFFM RHALKHMKEGSSII
Sbjct: 121 VKAYGRIDILVNSAAEQHKTNSVEEIDEKRIERVFRTNIFSQFFMVRHALKHMKEGSSII 180

Query: 181 NTTSVVAYKGSPQLLDYTATKGAIVAFTRGLALELVSRGIRVNGVAPGPIWTPLIPASVD 240
           NTTSVVAYKG+P+LLDYTATKGAIVAFTRGLAL+L +RGIRVNGVAPGPIWTPLIPAS D
Sbjct: 181 NTTSVVAYKGNPKLLDYTATKGAIVAFTRGLALQLANRGIRVNGVAPGPIWTPLIPASFD 240

Query: 241 EEEVRRFGSHVPMKRAGQPVEVAPSYVFLACNHCSSYFTGQILHPNGGTIVNG 293
            E++  FGS VPMKRAGQP+EVAPSYVFLACNHCSSYFTGQILHPNGG+IVNG
Sbjct: 241 NEDIESFGSEVPMKRAGQPIEVAPSYVFLACNHCSSYFTGQILHPNGGSIVNG 293

[Close]

BLAST of MC06g_new0498 vs. ExPASy TrEMBL
Match: A0A0A0LYH7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G570090 PE=4 SV=1)

HSP 1 Score: 498 bits (1281), Expect = 1.70e-176
Identity = 242/287 (84.32%), Postives = 263/287 (91.64%), Query Frame = 0


Query: 1   MATEGQKFAPQKQPTQPGKEYIMDPPPQFSSFQYKPSNKLLGKVALVTGGDSGIGRAVCH 60
           MATEGQ F PQKQ TQPGKE++MDP PQF+S QYKPSNKLLGKVALVTGGDSGIGRAVCH
Sbjct: 1   MATEGQTFPPQKQQTQPGKEHVMDPSPQFTSSQYKPSNKLLGKVALVTGGDSGIGRAVCH 60

Query: 61  CFALEGATVAFTYVKKEEEKDAQDMLQMLLESQAANAKRPIAIAVDLGFDDNCRRRVEEV 120
           CFALEGATVAFTYVKK+E+KDA+DML++L E QA +AK+PIA+  DLGFD++C+R V+EV
Sbjct: 61  CFALEGATVAFTYVKKQEDKDAKDMLEILRECQAPDAKQPIAVGADLGFDEDCKRVVDEV 120

Query: 121 VAAYGRIDILVNTAAEPHKAESVEEIDEQRLERVFRTNIFSQFFMARHALKHMKEGSSII 180
           V AYGRIDILVN AAE HK  SVEEIDEQR+ERVFRTNIFSQFFM RHALKHMKEGSSII
Sbjct: 121 VKAYGRIDILVNAAAEQHKTNSVEEIDEQRIERVFRTNIFSQFFMVRHALKHMKEGSSII 180

Query: 181 NTTSVVAYKGSPQLLDYTATKGAIVAFTRGLALELVSRGIRVNGVAPGPIWTPLIPASVD 240
           NTTSVVAYKG PQLLDY+ATKGAIVAFTRGLAL+L +RGIRVNGVAPGPIWTPLIPAS D
Sbjct: 181 NTTSVVAYKGCPQLLDYSATKGAIVAFTRGLALQLATRGIRVNGVAPGPIWTPLIPASFD 240

Query: 241 EEEVRRFGSHVPMKRAGQPVEVAPSYVFLACNHCSSYFTGQILHPNG 287
            EE+  FGS VPMKRAGQP+EVAPSYVFLACNHCSSYFTGQILHPNG
Sbjct: 241 NEEIESFGSEVPMKRAGQPIEVAPSYVFLACNHCSSYFTGQILHPNG 287

[Close]

BLAST of MC06g_new0498 vs. TAIR 10
Match: AT1G54870.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 443.7 bits (1140), Expect = 1.1e-124
Identity = 214/282 (75.89%), Postives = 248/282 (87.94%), Query Frame = 0


Query: 11  QKQPTQPGKEYIMDPPPQFSSFQYKPSNKLLGKVALVTGGDSGIGRAVCHCFALEGATVA 70
           QKQ  QPGKE++M+  PQFSS  Y+PSNKL GKVAL+TGGDSGIGRAV +CFA EGATVA
Sbjct: 53  QKQHAQPGKEHVMESSPQFSSSDYQPSNKLRGKVALITGGDSGIGRAVGYCFASEGATVA 112

Query: 71  FTYVKKEEEKDAQDMLQMLLESQAANAKRPIAIAVDLGFDDNCRRRVEEVVAAYGRIDIL 130
           FTYVK +EEKDAQ+ LQML E + +++K PIAI  DLGFD+NC+R V+EVV A+GRID+L
Sbjct: 113 FTYVKGQEEKDAQETLQMLKEVKTSDSKEPIAIPTDLGFDENCKRVVDEVVNAFGRIDVL 172

Query: 131 VNTAAEPHKAESVEEIDEQRLERVFRTNIFSQFFMARHALKHMKEGSSIINTTSVVAYKG 190
           +N AAE +++ ++EEIDE RLERVFRTNIFS FF+ RHALKHMKEGSSIINTTSV AYKG
Sbjct: 173 INNAAEQYESSTIEEIDEPRLERVFRTNIFSYFFLTRHALKHMKEGSSIINTTSVNAYKG 232

Query: 191 SPQLLDYTATKGAIVAFTRGLALELVSRGIRVNGVAPGPIWTPLIPASVDEEEVRRFGSH 250
           +  LLDYTATKGAIVAFTRGLAL+L  +GIRVNGVAPGPIWTPLIPAS +EE+++ FGS 
Sbjct: 233 NASLLDYTATKGAIVAFTRGLALQLAEKGIRVNGVAPGPIWTPLIPASFNEEKIKNFGSE 292

Query: 251 VPMKRAGQPVEVAPSYVFLACNHCSSYFTGQILHPNGGTIVN 293
           VPMKRAGQP+EVAPSYVFLACNHCSSYFTGQ+LHPNGG +VN
Sbjct: 293 VPMKRAGQPIEVAPSYVFLACNHCSSYFTGQVLHPNGGAVVN 334

[Close]

BLAST of MC06g_new0498 vs. TAIR 10
Match: AT3G05260.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 436.0 bits (1120), Expect = 2.3e-122
Identity = 211/285 (74.04%), Postives = 247/285 (86.67%), Query Frame = 0


Query: 8   FAPQKQPTQPGKEYIMDPPPQFSSFQYKPSNKLLGKVALVTGGDSGIGRAVCHCFALEGA 67
           F PQKQ TQPG +++M+P P+FSS  YKPSNKL GKVALVTGGDSGIG+AVCHC+ALEGA
Sbjct: 5   FPPQKQETQPGIQHVMEPTPEFSSSNYKPSNKLHGKVALVTGGDSGIGKAVCHCYALEGA 64

Query: 68  TVAFTYVKKEEEKDAQDMLQMLLESQAANAKRPIAIAVDLGFDDNCRRRVEEVVAAYGRI 127
           +VAFTYVK  E+KDA++ L++L E +   AK PI IA DLGF++NC+R VEEVV ++GRI
Sbjct: 65  SVAFTYVKGREDKDAEETLRLLHEVKTREAKEPIMIATDLGFEENCKRVVEEVVNSFGRI 124

Query: 128 DILVNTAAEPHKAESVEEIDEQRLERVFRTNIFSQFFMARHALKHMKEGSSIINTTSVVA 187
           D+LVN AAE H+  S+E+IDE RLERVFRTNIFSQFF+ ++ALKHMKEGSSIINTTSVVA
Sbjct: 125 DVLVNCAAEQHEV-SIEDIDEARLERVFRTNIFSQFFLVKYALKHMKEGSSIINTTSVVA 184

Query: 188 YKGSPQLLDYTATKGAIVAFTRGLALELVSRGIRVNGVAPGPIWTPLIPASVDEEEVRRF 247
           Y G+  LL+YTATKGAIV+FTRGLAL+L  +GIRVNGVAPGP+WTPLIPAS  EE +++F
Sbjct: 185 YAGNSSLLEYTATKGAIVSFTRGLALQLAPKGIRVNGVAPGPVWTPLIPASFSEEAIKQF 244

Query: 248 GSHVPMKRAGQPVEVAPSYVFLACNHCSSYFTGQILHPNGGTIVN 293
           GS  PMKRA QPVEVAPSYVFLACNHCSSY+TGQILHPNGG IVN
Sbjct: 245 GSETPMKRAAQPVEVAPSYVFLACNHCSSYYTGQILHPNGGLIVN 288

[Close]

BLAST of MC06g_new0498 vs. TAIR 10
Match: AT3G12800.1 (short-chain dehydrogenase-reductase B )

HSP 1 Score: 113.2 bits (282), Expect = 3.5e-25
Identity = 86/267 (32.21%), Postives = 137/267 (51.31%), Query Frame = 0


Query: 34  YKPSNKLLGKVALVTGGDSGIGRAVCHCFALEGATVAFTYVKKEEEKDAQDMLQMLLESQ 93
           +KP + + G+VAL+TGG SGIG  +   F   GA++A    +K+   DA   L+ L    
Sbjct: 5   FKP-DVVRGQVALITGGGSGIGFEISSQFGKHGASIAIMGRRKQVLDDAVSALRSL---- 64

Query: 94  AANAKRPIAIAVDLGFDDNCRRRVEEVVAAYGRIDILVNTAAEPHKAESVEEIDEQRLER 153
                + I +  D+   ++ RR VE     +G++DILVN AA    A + E++       
Sbjct: 65  ---GIQAIGLEGDVRKQEDARRVVEATFQHFGKLDILVNAAAGNFLA-AAEDLSPNGFRT 124

Query: 154 VFRTNIFSQFFMARHALKHMKEGS----------SIINTTSVVAYKGSPQLLDYTATKGA 213
           V   +    F M   ALK++K+G+          SIIN ++ + Y  S   +  +A K A
Sbjct: 125 VLDIDAVGTFNMCHAALKYLKKGAPGRDSSSGGGSIINISATLHYTASWYQIHVSAAKAA 184

Query: 214 IVAFTRGLALEL-VSRGIRVNGVAPGPI-WTPLIPASVDEEEVRRFGSHVPMKRAGQPVE 273
           + A TR LALE      IRVNG+APGPI  TP +   V EE   +   ++P+ + G+  +
Sbjct: 185 VDATTRNLALEWGTDYDIRVNGIAPGPIGGTPGMSKLVPEEIENKTREYMPLYKVGEKWD 244

Query: 274 VAPSYVFLACNHCSSYFTGQILHPNGG 289
           +A + ++L+C+    Y +G  +  +GG
Sbjct: 245 IAMAALYLSCD-SGKYVSGLTMVVDGG 261

[Close]

BLAST of MC06g_new0498 vs. TAIR 10
Match: AT4G05530.1 (indole-3-butyric acid response 1 )

HSP 1 Score: 102.4 bits (254), Expect = 6.1e-22
Identity = 79/255 (30.98%), Postives = 122/255 (47.84%), Query Frame = 0


Query: 35  KPSNKLLGKVALVTGGDSGIGRAVCHCFALEGATVAFTYVKKEEEKDAQDMLQMLLESQA 94
           K   +L GKVA+VT    GIG  +   F LEGA+V  +  K+    +A       L+S+ 
Sbjct: 4   KLPRRLEGKVAIVTASTQGIGFGITERFGLEGASVVVSSRKQANVDEA----VAKLKSKG 63

Query: 95  ANAKRPIAIAVDLGFDDNCRRRVEEVVAAYGRIDILVNTAAEPHKAESVEEIDEQRLERV 154
            +A     I   +    + R  VE+ V  YG+IDI+V  AA     + +    E  L+++
Sbjct: 64  IDA---YGIVCHVSNAQHRRNLVEKTVQKYGKIDIVVCNAAANPSTDPILSSKEAVLDKL 123

Query: 155 FRTNIFSQFFMARHALKHMKEGSSIINTTSVVAYKGSPQLLDYTATKGAIVAFTRGLALE 214
           +  N+ S   + +    H+++GSS+I  TS+  +     +  Y  TK A++  T+ LA E
Sbjct: 124 WEINVKSSILLLQDMAPHLEKGSSVIFITSIAGFSPQGAMAMYGVTKTALLGLTKALAAE 183

Query: 215 LVSRGIRVNGVAPGPIWTPLIPASVDEEEVRR-FGSHVPMKRAGQPVEVAPSYVFLACNH 274
           + +   RVN VAPG + T          EVR        + R G   ++A +  FLA + 
Sbjct: 184 M-APDTRVNAVAPGFVPTHFASFITGSSEVREGIEEKTLLNRLGTTGDMAAAAAFLASDD 243

Query: 275 CSSYFTGQILHPNGG 289
            SSY TG+ L   GG
Sbjct: 244 -SSYITGETLVVAGG 249

[Close]

BLAST of MC06g_new0498 vs. TAIR 10
Match: AT1G63380.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 100.5 bits (249), Expect = 2.3e-21
Identity = 75/268 (27.99%), Postives = 130/268 (48.51%), Query Frame = 0


Query: 33  QYKPSNKLLGKVALVTGGDSGIGRAVCHCFALEGATVAFTYVKKEEEKDAQDMLQMLLES 92
           Q +P  +L  KV LVTG  SGIGR +C      G  +    V      D  + L   + S
Sbjct: 10  QLEPWCELKDKVVLVTGASSGIGREICLDLCKAGCKI----VAAARRVDRLNSLCSEINS 69

Query: 93  QAANAKRPIAIAVDLGFD-DNCRRRVEEVVAAYGRIDILVNTAAEPHKAESVEEIDEQRL 152
             A   + +A+ +D+  + D  R+ V+E    +G+ID+L+N A      +S  ++ E+  
Sbjct: 70  FGAIGVQAVALELDVSSEADTIRKAVKEAWETFGKIDVLINNAGIRGNVKSSLDLSEEEW 129

Query: 153 ERVFRTNIFSQFFMARHA---LKHMKEGSSIINTTSVVA-YKGSPQ-LLDYTATKGAIVA 212
           ++VFRTN+   + ++++    ++  + G S+IN +S+   ++G  +  L Y  +KG +  
Sbjct: 130 DKVFRTNLTGSWLISKYVCLLMRDAERGGSVINVSSISGLHRGLLRGGLAYACSKGGVDT 189

Query: 213 FTRGLALELVSRGIRVNGVAPGPIWTPLIPASVDEEEVRRFGSHVPMKRAGQPVEVA-PS 272
            TR +A+EL    IRVN +APG   + +      +E +++    V   +  Q V+    S
Sbjct: 190 MTRMMAIELAVYKIRVNSIAPGIFRSEITQGLFQKEWLKKVTEKVVPLKMQQTVDPGLTS 249

Query: 273 YVFLACNHCSSYFTGQILHPNGGTIVNG 294
            V    +  S Y TG     + GT + G
Sbjct: 250 LVRYLIHDSSQYVTGNTYIVDSGTTLPG 273

The following BLAST results are available for this feature:

BLAST of MC06g_new0498 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FZ42	1.6e-123	75.89	NADPH-dependent aldehyde reductase 1, chloroplastic OS=Arabidopsis thaliana OX=3...	[more]
Q9MA93	3.3e-121	74.04	Glucose and ribitol dehydrogenase homolog 2 OS=Arabidopsis thaliana OX=3702 GN=A...	[more]
Q5KTS5	1.2e-118	71.77	Glucose and ribitol dehydrogenase OS=Daucus carota OX=4039 GN=CAISE5 PE=2 SV=1	[more]
Q75KH3	5.8e-102	66.11	Glucose and ribitol dehydrogenase homolog OS=Oryza sativa subsp. japonica OX=399...	[more]
P40397	3.0e-74	53.40	Uncharacterized oxidoreductase YhxC OS=Bacillus subtilis (strain 168) OX=224308 ...	[more]

BLAST of MC06g_new0498 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022156046.1	2.72e-211	100.00	NADPH-dependent aldehyde reductase 1, chloroplastic [Momordica charantia]	[more]
XP_023530263.1	7.42e-184	84.98	NADPH-dependent aldehyde reductase 1, chloroplastic-like [Cucurbita pepo subsp. ...	[more]
KAG6588473.1	1.61e-183	84.98	NADPH-dependent aldehyde reductase 1, chloroplastic, partial [Cucurbita argyrosp...	[more]
XP_022970115.1	4.29e-183	84.64	NADPH-dependent aldehyde reductase 1, chloroplastic-like [Cucurbita maxima]	[more]
XP_022926275.1	7.10e-182	84.30	NADPH-dependent aldehyde reductase 1, chloroplastic-like [Cucurbita moschata]	[more]

BLAST of MC06g_new0498 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DTN4	1.32e-211	100.00	NADPH-dependent aldehyde reductase 1, chloroplastic OS=Momordica charantia OX=36...	[more]
A0A6J1I1Y4	2.08e-183	84.64	NADPH-dependent aldehyde reductase 1, chloroplastic-like OS=Cucurbita maxima OX=...	[more]
A0A6J1EKN0	3.44e-182	84.30	NADPH-dependent aldehyde reductase 1, chloroplastic-like OS=Cucurbita moschata O...	[more]
A0A1S3BQQ8	5.44e-179	83.28	glucose and ribitol dehydrogenase homolog 1 OS=Cucumis melo OX=3656 GN=LOC103492...	[more]
A0A0A0LYH7	1.70e-176	84.32	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G570090 PE=4 SV=1	[more]

BLAST of MC06g_new0498 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G54870.1	1.1e-124	75.89	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G05260.1	2.3e-122	74.04	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G12800.1	3.5e-25	32.21	short-chain dehydrogenase-reductase B	[more]
AT4G05530.1	6.1e-22	30.98	indole-3-butyric acid response 1	[more]
AT1G63380.1	2.3e-21	27.99	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 125..136 score: 45.86 coord: 197..216 score: 39.91 coord: 177..185 score: 43.67
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 44..61 score: 50.2 coord: 171..187 score: 28.1 coord: 252..272 score: 36.52 coord: 197..216 score: 41.52 coord: 125..136 score: 48.18 coord: 218..235 score: 48.65
None	No IPR available	GENE3D	3.40.50.720		coord: 2..291 e-value: 7.5E-80 score: 270.0
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 52..288 e-value: 1.5E-54 score: 185.0
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 40..290 e-value: 1.7E-17 score: 60.5
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 46..241 e-value: 3.4E-12 score: 43.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	PANTHER	PTHR48107:SF6	GLUCOSE AND RIBITOL DEHYDROGENASE HOMOLOG 2	coord: 7..292
None	No IPR available	PANTHER	PTHR48107	NADPH-DEPENDENT ALDEHYDE REDUCTASE-LIKE PROTEIN, CHLOROPLASTIC-RELATED	coord: 7..292
None	No IPR available	CDD	cd05355	SDR_c1	coord: 17..292 e-value: 8.58423E-162 score: 449.05
IPR020904	Short-chain dehydrogenase/reductase, conserved site	PROSITE	PS00061	ADH_SHORT	coord: 184..212
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 42..290

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g_new0498.1	MC06g_new0498.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity