MC06g_new0397 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g_new0397
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	RNase H domain-containing protein
Location	MC06: 18217511 .. 18217951 (-)
RNA-Seq Expression	MC06g_new0397
Synteny	MC06g_new0397

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACTCGAATGGGAGCCAATCACATTGAGCAATGGGTGGCCCACTATATATATTCATTCTTACCGTGCACCTACTTCTCTACCGGCGAGTTCTGCTTCTGGGCAAGTTGCTCAGGTCGTTTGCAGGTGGCGAGCTCCTTCGGCCGATTGCTTTGAAGTAAATTTGGATGCGGCCTTCAATTCCGCTTCATCGCGTGCCGGTTTGGGAATCCTTGTTCGTGATTCTGCTGGGATTCTTCTGTTTGCTGTTGTAGTTTTCGTCCCCTCATGTGGTTCGGCTGATCAAGCTAAGGCGTTGGCCTTTCGGGACAACTTGGGATTGGTGTCGATATGGGCTTTAATCCTATCTGAGACTGATTCATTGAGCTGCTTTCGATTCTTGAAAGGCCATATGGAAGATTTCTTCCGAGGTAGGTCTGATTCTTTCTTACGTTCGTGA

mRNA sequence

ATGGAACTCGAATGGGAGCCAATCACATTGAGCAATGGGTGGCCCACTATATATATTCATTCTTACCGTGCACCTACTTCTCTACCGGCGAGTTCTGCTTCTGGGCAAGTTGCTCAGGTCGTTTGCAGGTGGCGAGCTCCTTCGGCCGATTGCTTTGAAGTAAATTTGGATGCGGCCTTCAATTCCGCTTCATCGCGTGCCGGTTTGGGAATCCTTGTTCGTGATTCTGCTGGGATTCTTCTGTTTGCTGTTGTAGTTTTCGTCCCCTCATGTGGTTCGGCTGATCAAGCTAAGGCGTTGGCCTTTCGGGACAACTTGGGATTGGTGTCGATATGGGCTTTAATCCTATCTGAGACTGATTCATTGAGCTGCTTTCGATTCTTGAAAGGCCATATGGAAGATTTCTTCCGAGGTAGGTCTGATTCTTTCTTACGTTCGTGA

Coding sequence (CDS)

ATGGAACTCGAATGGGAGCCAATCACATTGAGCAATGGGTGGCCCACTATATATATTCATTCTTACCGTGCACCTACTTCTCTACCGGCGAGTTCTGCTTCTGGGCAAGTTGCTCAGGTCGTTTGCAGGTGGCGAGCTCCTTCGGCCGATTGCTTTGAAGTAAATTTGGATGCGGCCTTCAATTCCGCTTCATCGCGTGCCGGTTTGGGAATCCTTGTTCGTGATTCTGCTGGGATTCTTCTGTTTGCTGTTGTAGTTTTCGTCCCCTCATGTGGTTCGGCTGATCAAGCTAAGGCGTTGGCCTTTCGGGACAACTTGGGATTGGTGTCGATATGGGCTTTAATCCTATCTGAGACTGATTCATTGAGCTGCTTTCGATTCTTGAAAGGCCATATGGAAGATTTCTTCCGAGGTAGGTCTGATTCTTTCTTACGTTCGTGA

Protein sequence

MELEWEPITLSNGWPTIYIHSYRAPTSLPASSASGQVAQVVCRWRAPSADCFEVNLDAAFNSASSRAGLGILVRDSAGILLFAVVVFVPSCGSADQAKALAFRDNLGLVSIWALILSETDSLSCFRFLKGHMEDFFRGRSDSFLRS

Homology

[Close]

BLAST of MC06g_new0397 vs. NCBI nr
Match: ABA96649.1 (hypothetical protein LOC_Os12g09520 [Oryza sativa Japonica Group])

HSP 1 Score: 62.4 bits (150), Expect = 3.13e-09
Identity = 32/84 (38.10%), Postives = 47/84 (55.95%), Query Frame = 0


Query: 29  PASSASGQVAQVVCRWRAPSADCFEVNLDAAFNSASSRAGLGILVRDSAGILLFAVVVFV 88
           P  S + QV +   RWRAP     ++N+D AFN      GLGI++RDS+G +L +   F+
Sbjct: 18  PPRSVTNQVKENNMRWRAPPVGWAKINVDGAFNPEDGLGGLGIIIRDSSGKVLLSSWRFI 77

Query: 89  PSCGSADQAKALAFRDNLGLVSIW 112
             C SA +++ LA  + L L   W
Sbjct: 78  RRCSSALESELLACLEGLKLAVEW 101

[Close]

BLAST of MC06g_new0397 vs. NCBI nr
Match: KAF2907024.1 (hypothetical protein DAI22_12g064750 [Oryza sativa Japonica Group])

HSP 1 Score: 62.4 bits (150), Expect = 4.29e-08
Identity = 32/84 (38.10%), Postives = 47/84 (55.95%), Query Frame = 0


Query: 29  PASSASGQVAQVVCRWRAPSADCFEVNLDAAFNSASSRAGLGILVRDSAGILLFAVVVFV 88
           P  S + QV +   RWRAP     ++N+D AFN      GLGI++RDS+G +L +   F+
Sbjct: 292 PPRSVTNQVKENNMRWRAPPVGWAKINVDGAFNPEDGLGGLGIIIRDSSGKVLLSSWRFI 351

Query: 89  PSCGSADQAKALAFRDNLGLVSIW 112
             C SA +++ LA  + L L   W
Sbjct: 352 RRCSSALESELLACLEGLKLAVEW 375

[Close]

BLAST of MC06g_new0397 vs. NCBI nr
Match: ABA97040.1 (retrotransposon protein, putative, unclassified [Oryza sativa Japonica Group])

HSP 1 Score: 62.4 bits (150), Expect = 5.14e-08
Identity = 33/105 (31.43%), Postives = 59/105 (56.19%), Query Frame = 0


Query: 20   HSYRAPTSLPASSASGQVAQVVCRWRAPSADCFEVNLDAAFNSASSRAGLGILVRDSAGI 79
            H  + PT +  S A+     V  RW  P     ++N+D +++S SS+ G+G+++RDS G 
Sbjct: 1772 HVVQQPTRM-RSEANHLRRVVPARWEKPQMGWMKLNVDGSYDSNSSKGGIGVVLRDSVGT 1831

Query: 80   LLFAVVVFVPSCGSADQAKALAFRDNLGLVSIWAL--ILSETDSL 122
            ++F+   F+  C S  +++ LA ++ + L   W L  I+ E+D L
Sbjct: 1832 VIFSACGFLERCSSPLESELLACKEGINLALQWTLLPIILESDCL 1875

[Close]

BLAST of MC06g_new0397 vs. NCBI nr
Match: EAY83065.1 (hypothetical protein OsI_38284 [Oryza sativa Indica Group])

HSP 1 Score: 61.2 bits (147), Expect = 7.88e-08
Identity = 28/89 (31.46%), Postives = 52/89 (58.43%), Query Frame = 0


Query: 43  RWRAPSADCFEVNLDAAFNSASSRAGLGILVRDSAGILLFAVVVFVPSCGSADQAKALAF 102
           RW  PS    ++N+D +F ++  + G+G ++RDS+G ++FA    +  CGSA +A+ LA 
Sbjct: 152 RWEKPSTGWMKLNVDGSFQASDGKGGIGAVLRDSSGNVIFAACGSMLVCGSAMEAELLAC 211

Query: 103 RDNLGLVSIWALI--LSETDSLSCFRFLK 129
           ++ + +   W  +  + ETD L  F  ++
Sbjct: 212 KEGIAMALQWTFLPFIVETDCLEVFNLIQ 240

[Close]

BLAST of MC06g_new0397 vs. NCBI nr
Match: MBA0826332.1 (hypothetical protein [Gossypium armourianum])

HSP 1 Score: 58.5 bits (140), Expect = 3.23e-07
Identity = 36/101 (35.64%), Postives = 55/101 (54.46%), Query Frame = 0


Query: 36  QVAQVVCRWRAPSADCFEVNLDAAFNSASSRAGLGILVRDSAGILLFAVVVFVPSCGSAD 95
           ++ Q V +W+ P     ++N DAA++  SS+A  GI+VR+  G  L +        GSA 
Sbjct: 25  KITQKVNKWKHPPDQTVKINFDAAYDERSSQAASGIVVRNREGKALISCSEIHHQIGSAF 84

Query: 96  QAKALAFRDN--LGLVSIWALILSETDSLSCFRFLKGHMED 134
            A+ALA R    +G+   W+ I+ E DSLS  +  K + ED
Sbjct: 85  AAEALACRRTVQIGIEMKWSKIIIEGDSLSIIKKCKENRED 125

[Close]

BLAST of MC06g_new0397 vs. ExPASy TrEMBL
Match: Q2QWF5 (RNase H domain-containing protein OS=Oryza sativa subsp. japonica OX=39947 GN=LOC_Os12g09520 PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.52e-09
Identity = 32/84 (38.10%), Postives = 47/84 (55.95%), Query Frame = 0


Query: 29  PASSASGQVAQVVCRWRAPSADCFEVNLDAAFNSASSRAGLGILVRDSAGILLFAVVVFV 88
           P  S + QV +   RWRAP     ++N+D AFN      GLGI++RDS+G +L +   F+
Sbjct: 18  PPRSVTNQVKENNMRWRAPPVGWAKINVDGAFNPEDGLGGLGIIIRDSSGKVLLSSWRFI 77

Query: 89  PSCGSADQAKALAFRDNLGLVSIW 112
             C SA +++ LA  + L L   W
Sbjct: 78  RRCSSALESELLACLEGLKLAVEW 101

[Close]

BLAST of MC06g_new0397 vs. ExPASy TrEMBL
Match: Q2QUC2 (Retrotransposon protein, putative, unclassified OS=Oryza sativa subsp. japonica OX=39947 GN=LOC_Os12g16750 PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 2.49e-08
Identity = 33/105 (31.43%), Postives = 59/105 (56.19%), Query Frame = 0


Query: 20   HSYRAPTSLPASSASGQVAQVVCRWRAPSADCFEVNLDAAFNSASSRAGLGILVRDSAGI 79
            H  + PT +  S A+     V  RW  P     ++N+D +++S SS+ G+G+++RDS G 
Sbjct: 1772 HVVQQPTRM-RSEANHLRRVVPARWEKPQMGWMKLNVDGSYDSNSSKGGIGVVLRDSVGT 1831

Query: 80   LLFAVVVFVPSCGSADQAKALAFRDNLGLVSIWAL--ILSETDSL 122
            ++F+   F+  C S  +++ LA ++ + L   W L  I+ E+D L
Sbjct: 1832 VIFSACGFLERCSSPLESELLACKEGINLALQWTLLPIILESDCL 1875

[Close]

BLAST of MC06g_new0397 vs. ExPASy TrEMBL
Match: A2ZKD1 (RNase H domain-containing protein OS=Oryza sativa subsp. indica OX=39946 GN=OsI_38284 PE=4 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 3.81e-08
Identity = 28/89 (31.46%), Postives = 52/89 (58.43%), Query Frame = 0


Query: 43  RWRAPSADCFEVNLDAAFNSASSRAGLGILVRDSAGILLFAVVVFVPSCGSADQAKALAF 102
           RW  PS    ++N+D +F ++  + G+G ++RDS+G ++FA    +  CGSA +A+ LA 
Sbjct: 152 RWEKPSTGWMKLNVDGSFQASDGKGGIGAVLRDSSGNVIFAACGSMLVCGSAMEAELLAC 211

Query: 103 RDNLGLVSIWALI--LSETDSLSCFRFLK 129
           ++ + +   W  +  + ETD L  F  ++
Sbjct: 212 KEGIAMALQWTFLPFIVETDCLEVFNLIQ 240

[Close]

BLAST of MC06g_new0397 vs. ExPASy TrEMBL
Match: A0A7J9IW15 (RNase H domain-containing protein OS=Gossypium armourianum OX=34283 GN=Goarm_011194 PE=4 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.56e-07
Identity = 36/101 (35.64%), Postives = 55/101 (54.46%), Query Frame = 0


Query: 36  QVAQVVCRWRAPSADCFEVNLDAAFNSASSRAGLGILVRDSAGILLFAVVVFVPSCGSAD 95
           ++ Q V +W+ P     ++N DAA++  SS+A  GI+VR+  G  L +        GSA 
Sbjct: 25  KITQKVNKWKHPPDQTVKINFDAAYDERSSQAASGIVVRNREGKALISCSEIHHQIGSAF 84

Query: 96  QAKALAFRDN--LGLVSIWALILSETDSLSCFRFLKGHMED 134
            A+ALA R    +G+   W+ I+ E DSLS  +  K + ED
Sbjct: 85  AAEALACRRTVQIGIEMKWSKIIIEGDSLSIIKKCKENRED 125

[Close]

BLAST of MC06g_new0397 vs. ExPASy TrEMBL
Match: A0A0P0W7W5 (Os04g0211600 protein OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0211600 PE=4 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.03e-07
Identity = 33/95 (34.74%), Postives = 50/95 (52.63%), Query Frame = 0


Query: 29  PASSASGQVAQVVCRWRAPSADCFEVNLDAAFNSASSRAGLGILVRDSAGILLFAVVVFV 88
           P + A    AQ + +W  P     ++N+DA FN     AG GI+ RD  G++L A    +
Sbjct: 5   PPARAEINAAQKM-KWEPPPEGVAKINVDAGFNKEMGEAGAGIIARDCRGLVLLAACKKL 64

Query: 89  PSCGSADQAKALAFRDNLGLVSIWAL--ILSETDS 121
             C SA QA+ALA  + + L + W    I+ E+D+
Sbjct: 65  SRCSSAAQAEALACLEGVQLAANWVQMPIILESDN 98

The following BLAST results are available for this feature:

BLAST of MC06g_new0397 vs.


Match Name	E-value	Identity	Description

BLAST of MC06g_new0397 vs. NCBI nr


Match Name	E-value	Identity	Description
ABA96649.1	3.13e-09	38.10	hypothetical protein LOC_Os12g09520 [Oryza sativa Japonica Group]	[more]
KAF2907024.1	4.29e-08	38.10	hypothetical protein DAI22_12g064750 [Oryza sativa Japonica Group]	[more]
ABA97040.1	5.14e-08	31.43	retrotransposon protein, putative, unclassified [Oryza sativa Japonica Group]	[more]
EAY83065.1	7.88e-08	31.46	hypothetical protein OsI_38284 [Oryza sativa Indica Group]	[more]
MBA0826332.1	3.23e-07	35.64	hypothetical protein [Gossypium armourianum]	[more]

BLAST of MC06g_new0397 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
Q2QWF5	1.52e-09	38.10	RNase H domain-containing protein OS=Oryza sativa subsp. japonica OX=39947 GN=LO...	[more]
Q2QUC2	2.49e-08	31.43	Retrotransposon protein, putative, unclassified OS=Oryza sativa subsp. japonica ...	[more]
A2ZKD1	3.81e-08	31.46	RNase H domain-containing protein OS=Oryza sativa subsp. indica OX=39946 GN=OsI_...	[more]
A0A7J9IW15	1.56e-07	35.64	RNase H domain-containing protein OS=Gossypium armourianum OX=34283 GN=Goarm_011...	[more]
A0A0P0W7W5	2.03e-07	34.74	Os04g0211600 protein OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0211600 PE...	[more]

BLAST of MC06g_new0397 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 55..131 e-value: 1.0E-5 score: 25.3

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g_new0397.1	MC06g_new0397.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity