MC06g_new0020 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g_new0020
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionProtein kinase domain-containing protein
LocationMC06: 665767 .. 668356 (-)
RNA-Seq ExpressionMC06g_new0020
SyntenyMC06g_new0020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGAACTTCTCCCTTTTCCCTCCTCCACGACACATCGGTCATCCAACTTTTCGATTGATTTCTGGCCCTGTTTTTGAGGTGTTCTTGTAGGCAGCACAAAGTACAATCGAAGCTCTCAATCATGTATTCTATTATTTCCCCTTTTCATTTGAGCTTATCCACTTGAAGATTACTGAACATTTTGATTCATTCCCTCTCTCTTTTAGTGATAATGCATACTGAATTGCTAGACTTCTGTGATTGACTTCTGTAGAAACATGGAAGCTGGGAGTAGATTTTACTCTGCTACGGATGAGTTCAGATTGGAAGCGAAATGGCTGATTGATCCCAAACATCTCTTCGTCGGACCTAGAATTGGAGAGGGAGCTCATGCCAAAGTTTACGAGGGCAAGTATGAACTCTTTGGAGATTTTTACTGGTTTCTGGGGTTTTGATTTTCTCCCAATTTTGCTTTGATGATTCTTCCAATTTGGTTGGCAACTCTAATATCTCGAGATCTAGAAATTCTTTTCGTATTTAATCAGAATTTCTACATGGGTTCTGCATTTTCATAAACTGAATGAGTTATTTCTAGCTGTTGATTCAGTTGTCCTTTGTATTTGGAATCCCAGAGTTCTGTGGGGATCAAAGTATAGCCTCGCAGTTTGCTATGTTTAACTGATGAATCACCAGTTTTTGTTCTCAATGTGCTTCAATTTTGTTTGCAGTGTGCTATGTTTACTAGTTTTATCTTGCGCTTACGCTTTATTTCACTGATTCCATTCAGATATAAGAACCAGACTGTGGCTATCAAAATTGTTCATAAAGGGGAAACAACTGACGAGATTGCAAAGAAAGAAGCTCGGTTTGCTCGAGAGGTTGCAATGTTGTCTAAAGTTCAACATAAAAATCTTGTCAAGGTTTGGTTCCATCCCAATCTACTGAGATTTTGTTTCGATCCTACATCTTCTGTATTATTTTACTTACAAATTATGTTCATTAACAATGTTTTTGAAGTATGCCATGAATGATTTAAGATTCATAATGTTGGATCCTTGTGTATCAACAGTTCATTGGTGCCTGCAAGGAGCCTGTAATGGTGATAGTAACCGAGCTTTTATTAGGAGGGACCTTACGAAAGTACCTGCTGAATATGCGTCCACGGTGCTTGGACACACACGTGGCAGTTGGTTTTGCACTCGACATTGCTCGTGCTATGGAATGCCTGCACTCTCATGGGATCATTCACCGCGATCTGAAACCTGGTACACTTTTTCCATCCACCAGATTATTCCTGCCGTTCCAAATGCTCTGTTACCAAACGTTTTCAAGTAATTATTGCATTTGTAATAATTTTGGTAGAACTTAAAATCACTGTAGAGTGCATCGCCGTTGGTTACTTTCTTAATTTCAAGATAAAGATCAAGTAACTTTTAGGCAGATGTTGGAGCTTAGTAAAGCATTTGTACATTCAAAATATCGACTTGGTCAGTTTCAATCATGGTGGTCGGTTTTTTAATTTTAAGTTTCACCCCTAGGCCGATGCCCTTCGCCTACTTGCTTGCTTGAATTCATTGCTTTCCTTACCAAGAACTTGACCTTACAAGGATATGATGTTTTCTCGAGTTCTACCTCCGCGTCCTTAGGAGACCGAGAACTCGAGGTAATTGGCTAGTTTGTCCATCACTTGCTTTTAGTCGCCAAACCACTTCAATAAGTGACGTGAAAAGATTTTTGGCATGAAAGGCATGCTCAAGGCCTCCATCTTCTTCTATCATCATCCAAGGATGATTAATGTGTTGAGCCTGAAATCTGTTTTAATGTTTTGGCAGAGAACCTATTGTTGACTGCAGATCACAAAACAGTAAAATTAGCTGATTTTGGTTTGGCAAGAGAAGAGTCATTGACGGAGATGATGACTGCAGAGACGGGGACGTACCGTTGGATGGCTCCAGAGGTGCTTGTTTTAGGAAATATGGTTTGGTTGTATGCATTCTGGTTGCTACTTGAGAAGAATGTTTCAAAGTAGCTTAATTGCATATTGTTATATGATGTCAGTTGTACAGCACCGTGACACTGAGGCAGGGGGAGAAGAAACATTACAACCATAAAGTGGATGCCTATAGCTTTGCCATTGTGTTATGGGAACTGCTACATAACAAGTTGCCATTTGAAGGCATGTCTAATCTTCAGGCAGCATATGCAGCTGCTTTTAAGGTAACCAATTCCTTCTTATGAACATCTATTTGCCACCATGTCGTCACTCTGATCGATGGATTTTCGGTCGTTTCAGAATGTGAGGCCTAGCGCGGAGAATCTCCCGGAGGAACTAGCCGATGTTCTAACATCGTGCTGGCAGGAGGATGCAAATGCTCGTCCAAATTTCACCCAAATTATCCACATGTTACTCAATTACCTTTACACCATATCACCCCCCGAGCCCGTGATTCCTTACCGAGTTTTCACTTCTGAGAATGCGGTGTTTCCTCCCGAGTCTCCTGGAACAAGCTCGCTGATGGCAGTCCGTGATGATTCAGGGGACACCCCAAAGGCAAAAATGGAAAATAAGCCCAGAGGTTGTTTCTTCTGCTCCAATGACTGTTACTAA

mRNA sequence

ATGAGGAACTTCTCCCTTTTCCCTCCTCCACGACACATCGGTCATCCAACTTTTCGATTGATTTCTGGCCCTGTTTTTGAGTTCATTGGTGCCTGCAAGGAGCCTGTAATGGTGATAGTAACCGAGCTTTTATTAGGAGGGACCTTACGAAAGTACCTGCTGAATATGCGTCCACGGTGCTTGGACACACACGTGGCAGTTGGTTTTGCACTCGACATTGCTCGTGCTATGGAATGCCTGCACTCTCATGGGATCATTCACCGCGATCTGAAACCTGAGAACCTATTGTTGACTGCAGATCACAAAACAGTAAAATTAGCTGATTTTGGTTTGGCAAGAGAAGAGTCATTGACGGAGATGATGACTGCAGAGACGGGGACGTACCGTTGGATGGCTCCAGAGTTGTACAGCACCGTGACACTGAGGCAGGGGGAGAAGAAACATTACAACCATAAAGTGGATGCCTATAGCTTTGCCATTGTGTTATGGGAACTGCTACATAACAAGTTGCCATTTGAAGGCATGTCTAATCTTCAGGCAGCATATGCAGCTGCTTTTAAGAATGTGAGGCCTAGCGCGGAGAATCTCCCGGAGGAACTAGCCGATGTTCTAACATCGTGCTGGCAGGAGGATGCAAATGCTCGTCCAAATTTCACCCAAATTATCCACATGTTACTCAATTACCTTTACACCATATCACCCCCCGAGCCCGTGATTCCTTACCGAGTTTTCACTTCTGAGAATGCGGTGTTTCCTCCCGAGTCTCCTGGAACAAGCTCGCTGATGGCAGTCCGTGATGATTCAGGGGACACCCCAAAGGCAAAAATGGAAAATAAGCCCAGAGGTTGTTTCTTCTGCTCCAATGACTGTTACTAA

Coding sequence (CDS)

ATGAGGAACTTCTCCCTTTTCCCTCCTCCACGACACATCGGTCATCCAACTTTTCGATTGATTTCTGGCCCTGTTTTTGAGTTCATTGGTGCCTGCAAGGAGCCTGTAATGGTGATAGTAACCGAGCTTTTATTAGGAGGGACCTTACGAAAGTACCTGCTGAATATGCGTCCACGGTGCTTGGACACACACGTGGCAGTTGGTTTTGCACTCGACATTGCTCGTGCTATGGAATGCCTGCACTCTCATGGGATCATTCACCGCGATCTGAAACCTGAGAACCTATTGTTGACTGCAGATCACAAAACAGTAAAATTAGCTGATTTTGGTTTGGCAAGAGAAGAGTCATTGACGGAGATGATGACTGCAGAGACGGGGACGTACCGTTGGATGGCTCCAGAGTTGTACAGCACCGTGACACTGAGGCAGGGGGAGAAGAAACATTACAACCATAAAGTGGATGCCTATAGCTTTGCCATTGTGTTATGGGAACTGCTACATAACAAGTTGCCATTTGAAGGCATGTCTAATCTTCAGGCAGCATATGCAGCTGCTTTTAAGAATGTGAGGCCTAGCGCGGAGAATCTCCCGGAGGAACTAGCCGATGTTCTAACATCGTGCTGGCAGGAGGATGCAAATGCTCGTCCAAATTTCACCCAAATTATCCACATGTTACTCAATTACCTTTACACCATATCACCCCCCGAGCCCGTGATTCCTTACCGAGTTTTCACTTCTGAGAATGCGGTGTTTCCTCCCGAGTCTCCTGGAACAAGCTCGCTGATGGCAGTCCGTGATGATTCAGGGGACACCCCAAAGGCAAAAATGGAAAATAAGCCCAGAGGTTGTTTCTTCTGCTCCAATGACTGTTACTAA

Protein sequence

MRNFSLFPPPRHIGHPTFRLISGPVFEFIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTHVAVGFALDIARAMECLHSHGIIHRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEKKHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELADVLTSCWQEDANARPNFTQIIHMLLNYLYTISPPEPVIPYRVFTSENAVFPPESPGTSSLMAVRDDSGDTPKAKMENKPRGCFFCSNDCY
Homology
BLAST of MC06g_new0020 vs. ExPASy Swiss-Prot
Match: Q9ZQ31 (Serine/threonine-protein kinase STY13 OS=Arabidopsis thaliana OX=3702 GN=STY13 PE=1 SV=2)

HSP 1 Score: 184.9 bits (468), Expect = 1.3e-45
Identity = 97/204 (47.55%), Postives = 134/204 (65.69%), Query Frame = 0

Query: 25  VFEFIGACKEP-VMVIVTELLLGGTLRKYLLNMRPRCLDTHVAVGFALDIARAMECLHSH 84
           +  FIGAC++P V  IVTE   GG++R++L   + R +   +AV  ALD+AR M  +H  
Sbjct: 191 IVRFIGACRKPMVWCIVTEYAKGGSVRQFLTRRQNRAVPLKLAVKQALDVARGMAYVHGR 250

Query: 85  GIIHRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQ 144
             IHRDLK +NLL++AD K++K+ADFG+AR E  TE MT ETGTYRWMAPE+        
Sbjct: 251 NFIHRDLKSDNLLISAD-KSIKIADFGVARIEVQTEGMTPETGTYRWMAPEMI------- 310

Query: 145 GEKKHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAEN--LPEELA 204
            + + YN KVD YSF IVLWEL+   LPF+ M+ +QAA+A   + VRP+  N  LP  L+
Sbjct: 311 -QHRAYNQKVDVYSFGIVLWELITGLLPFQNMTAVQAAFAVVNRGVRPTVPNDCLP-VLS 370

Query: 205 DVLTSCWQEDANARPNFTQIIHML 226
           D++T CW  +   RP F +++ +L
Sbjct: 371 DIMTRCWDANPEVRPCFVEVVKLL 384

BLAST of MC06g_new0020 vs. ExPASy Swiss-Prot
Match: Q2MHE4 (Serine/threonine/tyrosine-protein kinase HT1 OS=Arabidopsis thaliana OX=3702 GN=HT1 PE=1 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 3.3e-41
Identity = 96/212 (45.28%), Postives = 123/212 (58.02%), Query Frame = 0

Query: 19  RLISGPVFEFIGACKE-PVMVIVTELLLGGTLRKYLLNMRPRCLDTHVAVGFALDIARAM 78
           RL    + +FI ACK+ PV  I+TE +  G LR YL    P  L     +  ALDI+R M
Sbjct: 141 RLFHPNIVQFIAACKKPPVYCIITEYMSQGNLRMYLNKKEPYSLSIETVLRLALDISRGM 200

Query: 79  ECLHSHGIIHRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYS 138
           E LHS G+IHRDLK  NLLL  D   VK+ADFG +  E+         GTYRWMAPE+  
Sbjct: 201 EYLHSQGVIHRDLKSNNLLLN-DEMRVKVADFGTSCLETQCREAKGNMGTYRWMAPEMI- 260

Query: 139 TVTLRQGEKKHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPS-AENL 198
                  ++K Y  KVD YSF IVLWEL    LPF+GM+ +QAA+A A KN RP    + 
Sbjct: 261 -------KEKPYTRKVDVYSFGIVLWELTTALLPFQGMTPVQAAFAVAEKNERPPLPASC 320

Query: 199 PEELADVLTSCWQEDANARPNFTQIIHMLLNY 229
              LA ++  CW E+ + RP+F+ I+ +L  Y
Sbjct: 321 QPALAHLIKRCWSENPSKRPDFSNIVAVLEKY 343

BLAST of MC06g_new0020 vs. ExPASy Swiss-Prot
Match: F4JTP5 (Serine/threonine-protein kinase STY46 OS=Arabidopsis thaliana OX=3702 GN=STY46 PE=1 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 2.8e-40
Identity = 93/203 (45.81%), Postives = 129/203 (63.55%), Query Frame = 0

Query: 25  VFEFIGAC-KEPVMVIVTELLLGGTLRKYLLNMRPRCLDTHVAVGFALDIARAMECLHSH 84
           V +FIGAC K P + IVTE + GG++  Y L+ +            A+DI + M  LH +
Sbjct: 347 VVQFIGACTKPPHLCIVTEFMPGGSVYDY-LHKQKGVFKLPTLFKVAIDICKGMSYLHQN 406

Query: 85  GIIHRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQ 144
            IIHRDLK  NLL+  +++ VK+ADFG+AR ++ T +MTAETGTYRWMAPE+        
Sbjct: 407 NIIHRDLKAANLLMD-ENEVVKVADFGVARVKAQTGVMTAETGTYRWMAPEVI------- 466

Query: 145 GEKKHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPS-AENLPEELAD 204
            E K Y+HK D +S+ IVLWELL  KLP+E M+ LQAA     K +RP+  +N   +LA+
Sbjct: 467 -EHKPYDHKADVFSYGIVLWELLTGKLPYEYMTPLQAAVGVVQKGLRPTIPKNTHPKLAE 526

Query: 205 VLTSCWQEDANARPNFTQIIHML 226
           +L   W+ D+  RP+F++II  L
Sbjct: 527 LLERLWEHDSTQRPDFSEIIEQL 539

BLAST of MC06g_new0020 vs. ExPASy Swiss-Prot
Match: Q8RWL6 (Serine/threonine-protein kinase STY17 OS=Arabidopsis thaliana OX=3702 GN=STY17 PE=1 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 8.2e-40
Identity = 92/217 (42.40%), Postives = 132/217 (60.83%), Query Frame = 0

Query: 25  VFEFIGAC-KEPVMVIVTELLLGGTLRKYLLNMRPRCLDTHVAVGFALDIARAMECLHSH 84
           V +FIGAC + P + IVTE +  G++  + L+           +  ALD+++ M  LH +
Sbjct: 349 VVQFIGACTRSPNLCIVTEFMTRGSIYDF-LHKHKGVFKIQSLLKVALDVSKGMNYLHQN 408

Query: 85  GIIHRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQ 144
            IIHRDLK  NLL+  +H+ VK+ADFG+AR ++ + +MTAETGTYRWMAPE+        
Sbjct: 409 NIIHRDLKTANLLMD-EHEVVKVADFGVARVQTESGVMTAETGTYRWMAPEVI------- 468

Query: 145 GEKKHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEE---- 204
            E K Y+H+ D +S+AIVLWELL  +LP+  ++ LQAA     K +RP    +P+E    
Sbjct: 469 -EHKPYDHRADVFSYAIVLWELLTGELPYSYLTPLQAAVGVVQKGLRP---KIPKETHPK 528

Query: 205 LADVLTSCWQEDANARPNFTQIIHMLLNYLYTISPPE 237
           L ++L  CWQ+D   RPNF +II ML   +  +   E
Sbjct: 529 LTELLEKCWQQDPALRPNFAEIIEMLNQLIREVGDDE 552

BLAST of MC06g_new0020 vs. ExPASy Swiss-Prot
Match: O22558 (Serine/threonine-protein kinase STY8 OS=Arabidopsis thaliana OX=3702 GN=STY8 PE=1 SV=2)

HSP 1 Score: 157.9 bits (398), Expect = 1.7e-37
Identity = 87/203 (42.86%), Postives = 122/203 (60.10%), Query Frame = 0

Query: 25  VFEFIGAC-KEPVMVIVTELLLGGTLRKYLLNMRPRCLDTHVAVGFALDIARAMECLHSH 84
           V +F+GAC + P + IVTE +  G++  + L+ +         +  ALD+A+ M  LH +
Sbjct: 343 VVQFLGACTRSPTLCIVTEFMARGSIYDF-LHKQKCAFKLQTLLKVALDVAKGMSYLHQN 402

Query: 85  GIIHRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQ 144
            IIHRDLK  NLL+  +H  VK+ADFG+AR +  + +MTAETGTYRWMAPE+        
Sbjct: 403 NIIHRDLKTANLLMD-EHGLVKVADFGVARVQIESGVMTAETGTYRWMAPEVI------- 462

Query: 145 GEKKHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPS-AENLPEELAD 204
            E K YNHK D +S+AIVLWELL   +P+  ++ LQAA     K +RP   +    ++  
Sbjct: 463 -EHKPYNHKADVFSYAIVLWELLTGDIPYAFLTPLQAAVGVVQKGLRPKIPKKTHPKVKG 522

Query: 205 VLTSCWQEDANARPNFTQIIHML 226
           +L  CW +D   RP F +II ML
Sbjct: 523 LLERCWHQDPEQRPLFEEIIEML 535

BLAST of MC06g_new0020 vs. NCBI nr
Match: XP_022135302.1 (serine/threonine-protein kinase HT1 [Momordica charantia])

HSP 1 Score: 545 bits (1403), Expect = 9.35e-194
Identity = 264/265 (99.62%), Postives = 265/265 (100.00%), Query Frame = 0

Query: 27  EFIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTHVAVGFALDIARAMECLHSHGII 86
           +FIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTHVAVGFALDIARAMECLHSHGII
Sbjct: 89  KFIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTHVAVGFALDIARAMECLHSHGII 148

Query: 87  HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK 146
           HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK
Sbjct: 149 HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK 208

Query: 147 KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELADVLTS 206
           KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELADVLTS
Sbjct: 209 KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELADVLTS 268

Query: 207 CWQEDANARPNFTQIIHMLLNYLYTISPPEPVIPYRVFTSENAVFPPESPGTSSLMAVRD 266
           CWQEDANARPNFTQIIHMLLNYLYTISPPEPVIPYRVFTSENAVFPPESPGTSSLMAVRD
Sbjct: 269 CWQEDANARPNFTQIIHMLLNYLYTISPPEPVIPYRVFTSENAVFPPESPGTSSLMAVRD 328

Query: 267 DSGDTPKAKMENKPRGCFFCSNDCY 291
           DSGDTPKAKMENKPRGCFFCSNDCY
Sbjct: 329 DSGDTPKAKMENKPRGCFFCSNDCY 353

BLAST of MC06g_new0020 vs. NCBI nr
Match: XP_038880294.1 (serine/threonine-protein kinase STY13-like isoform X2 [Benincasa hispida])

HSP 1 Score: 525 bits (1353), Expect = 2.31e-187
Identity = 255/265 (96.23%), Postives = 259/265 (97.74%), Query Frame = 0

Query: 27  EFIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTHVAVGFALDIARAMECLHSHGII 86
           +FIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDT VAVGFALDIARAMECLHSHGII
Sbjct: 12  KFIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTRVAVGFALDIARAMECLHSHGII 71

Query: 87  HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK 146
           HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK
Sbjct: 72  HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK 131

Query: 147 KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELADVLTS 206
           KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAEN+PEELA VLTS
Sbjct: 132 KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENIPEELAIVLTS 191

Query: 207 CWQEDANARPNFTQIIHMLLNYLYTISPPEPVIPYRVFTSENAVFPPESPGTSSLMAVRD 266
           CWQEDANARPNFTQII MLLNYLYTISPPEPVIP R+F+SEN VFPPESPGTSSLMAVRD
Sbjct: 192 CWQEDANARPNFTQIIQMLLNYLYTISPPEPVIPSRIFSSENTVFPPESPGTSSLMAVRD 251

Query: 267 DSGDTPKAKMENKPRGCFFCSNDCY 291
           DSGDTPKAKMEN PRGCFFCSNDCY
Sbjct: 252 DSGDTPKAKMENNPRGCFFCSNDCY 276

BLAST of MC06g_new0020 vs. NCBI nr
Match: XP_008464507.1 (PREDICTED: serine/threonine-protein kinase HT1 [Cucumis melo] >XP_008464508.1 PREDICTED: serine/threonine-protein kinase HT1 [Cucumis melo] >XP_011654301.1 serine/threonine-protein kinase STY13 [Cucumis sativus] >XP_031737950.1 serine/threonine-protein kinase STY13 [Cucumis sativus] >KAA0057817.1 serine/threonine-protein kinase HT1-like [Cucumis melo var. makuwa] >KGN63533.1 hypothetical protein Csa_013773 [Cucumis sativus] >TYJ98501.1 serine/threonine-protein kinase HT1-like [Cucumis melo var. makuwa])

HSP 1 Score: 526 bits (1354), Expect = 2.72e-186
Identity = 255/265 (96.23%), Postives = 259/265 (97.74%), Query Frame = 0

Query: 27  EFIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTHVAVGFALDIARAMECLHSHGII 86
           +FIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDT VAVGFALDIARAMECLHSHGII
Sbjct: 89  KFIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTRVAVGFALDIARAMECLHSHGII 148

Query: 87  HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK 146
           HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK
Sbjct: 149 HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK 208

Query: 147 KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELADVLTS 206
           KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELA +LTS
Sbjct: 209 KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELAIILTS 268

Query: 207 CWQEDANARPNFTQIIHMLLNYLYTISPPEPVIPYRVFTSENAVFPPESPGTSSLMAVRD 266
           CWQEDANARPNF+QII MLLNYLYTISPPEPVIP R+FTSEN VFPPESPGTSSLMAVRD
Sbjct: 269 CWQEDANARPNFSQIIQMLLNYLYTISPPEPVIPSRIFTSENTVFPPESPGTSSLMAVRD 328

Query: 267 DSGDTPKAKMENKPRGCFFCSNDCY 291
           DSGDTPKAKMEN PRGCFFCSNDCY
Sbjct: 329 DSGDTPKAKMENNPRGCFFCSNDCY 353

BLAST of MC06g_new0020 vs. NCBI nr
Match: XP_038880293.1 (serine/threonine-protein kinase STY13-like isoform X1 [Benincasa hispida])

HSP 1 Score: 525 bits (1353), Expect = 3.86e-186
Identity = 255/265 (96.23%), Postives = 259/265 (97.74%), Query Frame = 0

Query: 27  EFIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTHVAVGFALDIARAMECLHSHGII 86
           +FIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDT VAVGFALDIARAMECLHSHGII
Sbjct: 89  KFIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTRVAVGFALDIARAMECLHSHGII 148

Query: 87  HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK 146
           HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK
Sbjct: 149 HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK 208

Query: 147 KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELADVLTS 206
           KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAEN+PEELA VLTS
Sbjct: 209 KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENIPEELAIVLTS 268

Query: 207 CWQEDANARPNFTQIIHMLLNYLYTISPPEPVIPYRVFTSENAVFPPESPGTSSLMAVRD 266
           CWQEDANARPNFTQII MLLNYLYTISPPEPVIP R+F+SEN VFPPESPGTSSLMAVRD
Sbjct: 269 CWQEDANARPNFTQIIQMLLNYLYTISPPEPVIPSRIFSSENTVFPPESPGTSSLMAVRD 328

Query: 267 DSGDTPKAKMENKPRGCFFCSNDCY 291
           DSGDTPKAKMEN PRGCFFCSNDCY
Sbjct: 329 DSGDTPKAKMENNPRGCFFCSNDCY 353

BLAST of MC06g_new0020 vs. NCBI nr
Match: XP_022987238.1 (serine/threonine-protein kinase HT1-like [Cucurbita maxima] >XP_022987239.1 serine/threonine-protein kinase HT1-like [Cucurbita maxima] >XP_022987240.1 serine/threonine-protein kinase HT1-like [Cucurbita maxima])

HSP 1 Score: 509 bits (1312), Expect = 6.76e-180
Identity = 249/265 (93.96%), Postives = 255/265 (96.23%), Query Frame = 0

Query: 27  EFIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTHVAVGFALDIARAMECLHSHGII 86
           +FIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDT VAVGFALDIARAMECLHSHGII
Sbjct: 89  KFIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTRVAVGFALDIARAMECLHSHGII 148

Query: 87  HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK 146
           HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK
Sbjct: 149 HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK 208

Query: 147 KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELADVLTS 206
           KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELA VLTS
Sbjct: 209 KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELAMVLTS 268

Query: 207 CWQEDANARPNFTQIIHMLLNYLYTISPPEPVIPYRVFTSENAVFPPESPGTSSLMAVRD 266
           CWQEDANARPNF+QII MLLNYLYTISPPEP+I  R F+SEN VFPPESPGTSSLMAVRD
Sbjct: 269 CWQEDANARPNFSQIIQMLLNYLYTISPPEPMIASRFFSSENTVFPPESPGTSSLMAVRD 328

Query: 267 DSGDTPKAKMENKPRGCFFCSNDCY 291
           DSG+TPKAKMEN PRGCF CS DC+
Sbjct: 329 DSGETPKAKMENNPRGCFSCSYDCF 353

BLAST of MC06g_new0020 vs. ExPASy TrEMBL
Match: A0A6J1C2B7 (serine/threonine-protein kinase HT1 OS=Momordica charantia OX=3673 GN=LOC111007297 PE=3 SV=1)

HSP 1 Score: 545 bits (1403), Expect = 4.53e-194
Identity = 264/265 (99.62%), Postives = 265/265 (100.00%), Query Frame = 0

Query: 27  EFIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTHVAVGFALDIARAMECLHSHGII 86
           +FIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTHVAVGFALDIARAMECLHSHGII
Sbjct: 89  KFIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTHVAVGFALDIARAMECLHSHGII 148

Query: 87  HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK 146
           HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK
Sbjct: 149 HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK 208

Query: 147 KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELADVLTS 206
           KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELADVLTS
Sbjct: 209 KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELADVLTS 268

Query: 207 CWQEDANARPNFTQIIHMLLNYLYTISPPEPVIPYRVFTSENAVFPPESPGTSSLMAVRD 266
           CWQEDANARPNFTQIIHMLLNYLYTISPPEPVIPYRVFTSENAVFPPESPGTSSLMAVRD
Sbjct: 269 CWQEDANARPNFTQIIHMLLNYLYTISPPEPVIPYRVFTSENAVFPPESPGTSSLMAVRD 328

Query: 267 DSGDTPKAKMENKPRGCFFCSNDCY 291
           DSGDTPKAKMENKPRGCFFCSNDCY
Sbjct: 329 DSGDTPKAKMENKPRGCFFCSNDCY 353

BLAST of MC06g_new0020 vs. ExPASy TrEMBL
Match: A0A1S3CN64 (serine/threonine-protein kinase HT1 OS=Cucumis melo OX=3656 GN=LOC103502370 PE=3 SV=1)

HSP 1 Score: 526 bits (1354), Expect = 1.32e-186
Identity = 255/265 (96.23%), Postives = 259/265 (97.74%), Query Frame = 0

Query: 27  EFIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTHVAVGFALDIARAMECLHSHGII 86
           +FIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDT VAVGFALDIARAMECLHSHGII
Sbjct: 89  KFIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTRVAVGFALDIARAMECLHSHGII 148

Query: 87  HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK 146
           HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK
Sbjct: 149 HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK 208

Query: 147 KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELADVLTS 206
           KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELA +LTS
Sbjct: 209 KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELAIILTS 268

Query: 207 CWQEDANARPNFTQIIHMLLNYLYTISPPEPVIPYRVFTSENAVFPPESPGTSSLMAVRD 266
           CWQEDANARPNF+QII MLLNYLYTISPPEPVIP R+FTSEN VFPPESPGTSSLMAVRD
Sbjct: 269 CWQEDANARPNFSQIIQMLLNYLYTISPPEPVIPSRIFTSENTVFPPESPGTSSLMAVRD 328

Query: 267 DSGDTPKAKMENKPRGCFFCSNDCY 291
           DSGDTPKAKMEN PRGCFFCSNDCY
Sbjct: 329 DSGDTPKAKMENNPRGCFFCSNDCY 353

BLAST of MC06g_new0020 vs. ExPASy TrEMBL
Match: A0A5D3BII5 (Serine/threonine-protein kinase HT1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001040 PE=3 SV=1)

HSP 1 Score: 526 bits (1354), Expect = 1.32e-186
Identity = 255/265 (96.23%), Postives = 259/265 (97.74%), Query Frame = 0

Query: 27  EFIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTHVAVGFALDIARAMECLHSHGII 86
           +FIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDT VAVGFALDIARAMECLHSHGII
Sbjct: 89  KFIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTRVAVGFALDIARAMECLHSHGII 148

Query: 87  HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK 146
           HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK
Sbjct: 149 HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK 208

Query: 147 KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELADVLTS 206
           KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELA +LTS
Sbjct: 209 KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELAIILTS 268

Query: 207 CWQEDANARPNFTQIIHMLLNYLYTISPPEPVIPYRVFTSENAVFPPESPGTSSLMAVRD 266
           CWQEDANARPNF+QII MLLNYLYTISPPEPVIP R+FTSEN VFPPESPGTSSLMAVRD
Sbjct: 269 CWQEDANARPNFSQIIQMLLNYLYTISPPEPVIPSRIFTSENTVFPPESPGTSSLMAVRD 328

Query: 267 DSGDTPKAKMENKPRGCFFCSNDCY 291
           DSGDTPKAKMEN PRGCFFCSNDCY
Sbjct: 329 DSGDTPKAKMENNPRGCFFCSNDCY 353

BLAST of MC06g_new0020 vs. ExPASy TrEMBL
Match: A0A0A0LP20 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G003510 PE=3 SV=1)

HSP 1 Score: 526 bits (1354), Expect = 1.32e-186
Identity = 255/265 (96.23%), Postives = 259/265 (97.74%), Query Frame = 0

Query: 27  EFIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTHVAVGFALDIARAMECLHSHGII 86
           +FIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDT VAVGFALDIARAMECLHSHGII
Sbjct: 89  KFIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTRVAVGFALDIARAMECLHSHGII 148

Query: 87  HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK 146
           HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK
Sbjct: 149 HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK 208

Query: 147 KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELADVLTS 206
           KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELA +LTS
Sbjct: 209 KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELAIILTS 268

Query: 207 CWQEDANARPNFTQIIHMLLNYLYTISPPEPVIPYRVFTSENAVFPPESPGTSSLMAVRD 266
           CWQEDANARPNF+QII MLLNYLYTISPPEPVIP R+FTSEN VFPPESPGTSSLMAVRD
Sbjct: 269 CWQEDANARPNFSQIIQMLLNYLYTISPPEPVIPSRIFTSENTVFPPESPGTSSLMAVRD 328

Query: 267 DSGDTPKAKMENKPRGCFFCSNDCY 291
           DSGDTPKAKMEN PRGCFFCSNDCY
Sbjct: 329 DSGDTPKAKMENNPRGCFFCSNDCY 353

BLAST of MC06g_new0020 vs. ExPASy TrEMBL
Match: A0A6J1JIV9 (serine/threonine-protein kinase HT1-like OS=Cucurbita maxima OX=3661 GN=LOC111484853 PE=3 SV=1)

HSP 1 Score: 509 bits (1312), Expect = 3.27e-180
Identity = 249/265 (93.96%), Postives = 255/265 (96.23%), Query Frame = 0

Query: 27  EFIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTHVAVGFALDIARAMECLHSHGII 86
           +FIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDT VAVGFALDIARAMECLHSHGII
Sbjct: 89  KFIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTRVAVGFALDIARAMECLHSHGII 148

Query: 87  HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK 146
           HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK
Sbjct: 149 HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK 208

Query: 147 KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELADVLTS 206
           KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELA VLTS
Sbjct: 209 KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELAMVLTS 268

Query: 207 CWQEDANARPNFTQIIHMLLNYLYTISPPEPVIPYRVFTSENAVFPPESPGTSSLMAVRD 266
           CWQEDANARPNF+QII MLLNYLYTISPPEP+I  R F+SEN VFPPESPGTSSLMAVRD
Sbjct: 269 CWQEDANARPNFSQIIQMLLNYLYTISPPEPMIASRFFSSENTVFPPESPGTSSLMAVRD 328

Query: 267 DSGDTPKAKMENKPRGCFFCSNDCY 291
           DSG+TPKAKMEN PRGCF CS DC+
Sbjct: 329 DSGETPKAKMENNPRGCFSCSYDCF 353

BLAST of MC06g_new0020 vs. TAIR 10
Match: AT5G50180.1 (Protein kinase superfamily protein )

HSP 1 Score: 454.9 bits (1169), Expect = 4.8e-128
Identity = 218/265 (82.26%), Postives = 235/265 (88.68%), Query Frame = 0

Query: 27  EFIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTHVAVGFALDIARAMECLHSHGII 86
           +FIGACKEPVMVIVTELL GGTLRKYLLN+RP CL+T VA+GFALDIAR MECLHSHGII
Sbjct: 82  KFIGACKEPVMVIVTELLQGGTLRKYLLNLRPACLETRVAIGFALDIARGMECLHSHGII 141

Query: 87  HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK 146
           HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLR GEK
Sbjct: 142 HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRLGEK 201

Query: 147 KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELADVLTS 206
           KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAE+LPEEL D++TS
Sbjct: 202 KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAESLPEELGDIVTS 261

Query: 207 CWQEDANARPNFTQIIHMLLNYLYTISPPEPVIPYRVFTSENAVFPPESPGTSSLMAVRD 266
           CW ED NARPNFT II +LLNYL  +  P   IP R+  S+N + PP+SPGTSSLMA  D
Sbjct: 262 CWNEDPNARPNFTHIIELLLNYLSKVGSPISAIPQRILASKNTLLPPDSPGTSSLMAKLD 321

Query: 267 DSGDTPKAKMENKPRGCFFCSNDCY 292
           + G+TPKAK E+K +G FFC N CY
Sbjct: 322 ECGETPKAKSEDKRKGLFFCFNQCY 346

BLAST of MC06g_new0020 vs. TAIR 10
Match: AT5G40540.1 (Protein kinase superfamily protein )

HSP 1 Score: 409.8 bits (1052), Expect = 1.8e-114
Identity = 206/262 (78.63%), Postives = 228/262 (87.02%), Query Frame = 0

Query: 27  EFIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTHVAVGFALDIARAMECLHSHGII 86
           +FIGACKEP+MVIVTELLLGGTLRKYL+++RP  LD  VAVG+ALDIARAMECLHSHG+I
Sbjct: 88  KFIGACKEPIMVIVTELLLGGTLRKYLVSLRPGSLDIRVAVGYALDIARAMECLHSHGVI 147

Query: 87  HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK 146
           HRDLKPE+L+LTAD+KTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLR GEK
Sbjct: 148 HRDLKPESLILTADYKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRHGEK 207

Query: 147 KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELADVLTS 206
           KHYNHKVDAYSFAIVLWEL+HNKLPFEGMSNLQAAYAAAFKNVRPSA++LP++LA ++TS
Sbjct: 208 KHYNHKVDAYSFAIVLWELIHNKLPFEGMSNLQAAYAAAFKNVRPSADDLPKDLAMIVTS 267

Query: 207 CWQEDANARPNFTQIIHMLLNYLYTISPPEPVIP--YRVFTSENAVFPPESPGTSSLMAV 266
           CW+ED N RPNFT+II MLL  L TIS  E V P   RVF+SEN V PPESPGT SLM V
Sbjct: 268 CWKEDPNDRPNFTEIIQMLLRCLSTISSTELVPPAIKRVFSSENTVLPPESPGTCSLMTV 327

Query: 267 RD-DSGDTPKAKMENKPRGCFF 286
           RD D   T     +N+ RG FF
Sbjct: 328 RDKDQIPTDANSAQNEVRGSFF 349

BLAST of MC06g_new0020 vs. TAIR 10
Match: AT3G27560.1 (Protein kinase superfamily protein )

HSP 1 Score: 405.2 bits (1040), Expect = 4.4e-113
Identity = 204/267 (76.40%), Postives = 230/267 (86.14%), Query Frame = 0

Query: 27  EFIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTHVAVGFALDIARAMECLHSHGII 86
           +FIGACKEP+MVIVTELLLGGTLRKYL+++RP+ LD  +AVGFALDIARAMECLHSHGII
Sbjct: 88  KFIGACKEPMMVIVTELLLGGTLRKYLVSLRPKRLDIRLAVGFALDIARAMECLHSHGII 147

Query: 87  HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK 146
           HRDLKPENL+L+ADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK
Sbjct: 148 HRDLKPENLILSADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK 207

Query: 147 KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELADVLTS 206
           KHYNHKVDAYSFAIVLWEL+ NKLPFEGMSNLQAAYAAAFKN+RPSAE+LP +L  ++TS
Sbjct: 208 KHYNHKVDAYSFAIVLWELILNKLPFEGMSNLQAAYAAAFKNLRPSAEDLPGDLEMIVTS 267

Query: 207 CWQEDANARPNFTQIIHMLLNYLYTISPPEPVIP--YRVFTSENAVFPPESPGTSSLMAV 266
           CW+ED N RPNFT+II MLL YL T+S P+ + P   RVF+SEN V  PESPGT SLM+V
Sbjct: 268 CWKEDPNERPNFTEIIQMLLRYLTTVSAPQIIPPPNRRVFSSENIVLSPESPGTCSLMSV 327

Query: 267 RDDSGDTPKAKM------ENKPRGCFF 286
           RD  GD  +  +      E + +G FF
Sbjct: 328 RD--GDVSRQTVNTADSSEKQTKGSFF 352

BLAST of MC06g_new0020 vs. TAIR 10
Match: AT5G01850.1 (Protein kinase superfamily protein )

HSP 1 Score: 313.2 bits (801), Expect = 2.3e-85
Identity = 152/212 (71.70%), Postives = 180/212 (84.91%), Query Frame = 0

Query: 27  EFIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTHVAVGFALDIARAMECLHSHGII 86
           +FIGACK+P+MVIVTELL G +LRKYL ++RP+ L   +A+ FALDIARA+ CLH++GII
Sbjct: 80  KFIGACKDPLMVIVTELLPGMSLRKYLTSIRPQLLHLPLALSFALDIARALHCLHANGII 139

Query: 87  HRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQGEK 146
           HRDLKP+NLLLT +HK+VKLADFGLAREES+TEMMTAETGTYRWMAPELYSTVTLRQGEK
Sbjct: 140 HRDLKPDNLLLTENHKSVKLADFGLAREESVTEMMTAETGTYRWMAPELYSTVTLRQGEK 199

Query: 147 KHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRP-SAENLPEELADVLT 206
           KHYN+KVD YSF IVLWELL N++PFEGMSNLQAAYAAAFK  RP   E +   LA ++ 
Sbjct: 200 KHYNNKVDVYSFGIVLWELLTNRMPFEGMSNLQAAYAAAFKQERPVMPEGISPSLAFIVQ 259

Query: 207 SCWQEDANARPNFTQIIHMLLNYLYTISPPEP 238
           SCW ED N RP+F+QII +L  +L T++PP P
Sbjct: 260 SCWVEDPNMRPSFSQIIRLLNEFLLTLTPPPP 291

BLAST of MC06g_new0020 vs. TAIR 10
Match: AT5G66710.1 (Protein kinase superfamily protein )

HSP 1 Score: 258.1 bits (658), Expect = 8.7e-69
Identity = 127/209 (60.77%), Postives = 160/209 (76.56%), Query Frame = 0

Query: 25  VFEFIGACKEPVMVIVTELLLGGTLRKYLLNMRPRCLDTHVAVGFALDIARAMECLHSHG 84
           +  FIGAC EP ++I+TEL+ G TL+K++L++RP+ LD  +++ FALDIAR ME L+++G
Sbjct: 132 IVRFIGACIEPKLMIITELMEGNTLQKFMLSVRPKPLDLKLSISFALDIARGMEFLNANG 191

Query: 85  IIHRDLKPENLLLTADHKTVKLADFGLAREESLTEMMTAETGTYRWMAPELYSTVTLRQG 144
           IIHRDLKP N+LLT D K VKLADFGLAREE+    MT E GTYRWMAPEL+S  TL  G
Sbjct: 192 IIHRDLKPSNMLLTGDQKHVKLADFGLAREET-KGFMTFEAGTYRWMAPELFSYDTLEIG 251

Query: 145 EKKHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAENLPEELADVL 204
           EKKHY+HKVD YSFAIV WELL NK PF+G +N+  AYAA+ KN RPS ENLPE +  +L
Sbjct: 252 EKKHYDHKVDVYSFAIVFWELLTNKTPFKGKNNIFVAYAAS-KNQRPSVENLPEGVVSIL 311

Query: 205 TSCWQEDANARPNFTQIIHMLLNYLYTIS 234
            SCW E+ +ARP F +I + L N L ++S
Sbjct: 312 QSCWAENPDARPEFKEITYSLTNLLRSLS 338

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZQ311.3e-4547.55Serine/threonine-protein kinase STY13 OS=Arabidopsis thaliana OX=3702 GN=STY13 P... [more]
Q2MHE43.3e-4145.28Serine/threonine/tyrosine-protein kinase HT1 OS=Arabidopsis thaliana OX=3702 GN=... [more]
F4JTP52.8e-4045.81Serine/threonine-protein kinase STY46 OS=Arabidopsis thaliana OX=3702 GN=STY46 P... [more]
Q8RWL68.2e-4042.40Serine/threonine-protein kinase STY17 OS=Arabidopsis thaliana OX=3702 GN=STY17 P... [more]
O225581.7e-3742.86Serine/threonine-protein kinase STY8 OS=Arabidopsis thaliana OX=3702 GN=STY8 PE=... [more]
Match NameE-valueIdentityDescription
XP_022135302.19.35e-19499.62serine/threonine-protein kinase HT1 [Momordica charantia][more]
XP_038880294.12.31e-18796.23serine/threonine-protein kinase STY13-like isoform X2 [Benincasa hispida][more]
XP_008464507.12.72e-18696.23PREDICTED: serine/threonine-protein kinase HT1 [Cucumis melo] >XP_008464508.1 PR... [more]
XP_038880293.13.86e-18696.23serine/threonine-protein kinase STY13-like isoform X1 [Benincasa hispida][more]
XP_022987238.16.76e-18093.96serine/threonine-protein kinase HT1-like [Cucurbita maxima] >XP_022987239.1 seri... [more]
Match NameE-valueIdentityDescription
A0A6J1C2B74.53e-19499.62serine/threonine-protein kinase HT1 OS=Momordica charantia OX=3673 GN=LOC1110072... [more]
A0A1S3CN641.32e-18696.23serine/threonine-protein kinase HT1 OS=Cucumis melo OX=3656 GN=LOC103502370 PE=3... [more]
A0A5D3BII51.32e-18696.23Serine/threonine-protein kinase HT1-like OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A0A0LP201.32e-18696.23Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G003... [more]
A0A6J1JIV93.27e-18093.96serine/threonine-protein kinase HT1-like OS=Cucurbita maxima OX=3661 GN=LOC11148... [more]
Match NameE-valueIdentityDescription
AT5G50180.14.8e-12882.26Protein kinase superfamily protein [more]
AT5G40540.11.8e-11478.63Protein kinase superfamily protein [more]
AT3G27560.14.4e-11376.40Protein kinase superfamily protein [more]
AT5G01850.12.3e-8571.70Protein kinase superfamily protein [more]
AT5G66710.18.7e-6960.77Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPRINTSPR00109TYRKINASEcoord: 79..97
score: 40.39
coord: 196..218
score: 35.48
coord: 41..54
score: 36.45
coord: 153..175
score: 26.61
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 25..225
e-value: 5.8E-44
score: 150.3
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 7..229
e-value: 1.4E-24
score: 97.7
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 1..226
score: 34.053768
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 18..232
e-value: 2.5E-56
score: 192.8
NoneNo IPR availablePIRSRPIRSR000606-51PIRSR000606-51coord: 39..178
e-value: 8.8E-20
score: 68.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 255..275
NoneNo IPR availablePANTHERPTHR23257SERINE-THREONINE PROTEIN KINASEcoord: 27..266
NoneNo IPR availablePANTHERPTHR23257:SF881SERINE/THREONINE-PROTEIN KINASE HT1-LIKEcoord: 27..266
NoneNo IPR availableCDDcd13999STKc_MAP3K-likecoord: 28..225
e-value: 2.89751E-84
score: 251.303
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 85..97
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 26..254

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g_new0020.1MC06g_new0020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity