MC06g2096 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g2096
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionoxalate--CoA ligase-like
LocationMC06: 28467682 .. 28471192 (+)
RNA-Seq ExpressionMC06g2096
SyntenyMC06g2096
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTATTTTATTTTAGTTCAATTACTTGATCAATATTTATACCTTAAATAAATCTTAAAAATGGTCACCCAATTGACTTAAGGTTAATTTTGATTTGATTTAACAAAAAGGAAAGGCACAACTTCCATTTTAGAAATAGCAAAATGTGAATAAGTTTCCCAAATGTTTAAAGGAAATATCTATACAAGAACTAATTTTGTTTGTTTTCAAAACTTAACCTTGGCCCGTGCAGACTATTTTATTAATTCATCACAAGCTTTAAAATTAGACTTAAACATTTAAAAATAATTAGACTTAAACATTTAAGAATATCTCAACTAACATAGAGTAAGCTCTATAGTAGAAGGATAAATGATAATTTAACAAATTTGTCATTATTCCTTTTAAAGATCATCAATTTGTAAATGTGGAAACGGTATTTAATTGGAAAATATCAGACAACATTTTTAAAGATGGAATTTTTGATCATTGAGTCATCTCATTTTAATGGGATCAAGTCCAAAATAAAGCGATAGGTGGGGCAGGCAAAACAAAGTTCATGAGACAACATATAAGTTGTGGAATCAAAATTTTTATTCATCAATTAGGCATAAAGCTTGTTTTAAAGAGTTCACTTAATTTGGTTTGTTTGGACGGAGTAGAGTGGCGTACAAGAGAGAGTCTAATCAATTTATTTTCGAACAGTCCCTCAAATCAAAATAAAAACAAGTTATGGGCCAAATAATAAAAGAAAAGAAAAGTTCAAAATAGTTTTGAAAACGAATTTTCTAGGAAAATCAAAAATCGAGAAGCCAAATTCTGTTTTGGCTGCTGACACGTGGAGAAAAAAGGAGTCCAGTTTCAACCTTCACGAGTCCTACCTCTCCAAACCACCTAAAAAATCCTATATTAATCCCTCATTTTCTCACCTTTGGAACACGCGTCTCAGTTCTTCTGATATCTGATCAAAGTTTTCCTCAGCCTTTGATTAATTTTTTTTAATTAATTGTTTTGTTTTTTGTTGTTTCATCTGGAAATCCAACTTCCCAGTAGTTTAATCGCGTTCATCAATTTTTGGAAGCGTTTCGATTTCTGAAATTAACAGAAACAGAGAGAGAGAGATGGAGAAGAGTACCTTGAGTGGGTTGTTGAAGAAGGCCGCCTCTGAGTTTCCTCGCCGGCGGGCTCTCTCAGTCTCCGGAAAGTTCGACCTGACACATGAGCGCTTGCAAGAGCTCGTCGAACATGCCGCGTCTCTCCTGGCGGATGCCGGAGTTAACGCCGGCGATGTGGTCGCCCTCACCTTCCATAACACTGTGGAGGTACGTCTTCGGTGAACTTCTTGTGATTTTTTTCCTTTTTTTTTCCCGCGGATTCTCTGGCTAAGCCCCTGTTTGGTTGCTGAGAAAATTTCGGTGAGAAACAGTAGAAAAAAGTGCCTGCATAGTGTCAATTACGCAATCTTAACGTCTTTTTTGTTTTTTGTGCTTTGATTCATGAGAATGAGAGCGAGAGGATTGATTGAATTAATTGATTTACCTTTACTTTTGTAAGAAGCTGATGATGATCAGATGTTTCCTCTTGCAGTTTGTGATCATGTTTCTGGCCGTAATCCGCTGCCGAGCCACGGCTGCGCCATTAAATCCGGCTTACACGGCTGAGGAGTTCGAGTTTTACTTATCGGACTCGGAATCGAAGTTTCTCCTGACGTCAGAAGAAGGGATTCCATCCGCTCAATCCGCCGCATTGAAGCTCAATATCCCTCAAGTAAAAGCAAGCCTGTCTGGCGACGATGAGTTTCTCAATCTTTCTCCGTCAGGAACCGAATCGAAATCGAACATGGCGGAAATCGTAAACGATCCGTCCGATGCGGCGCTCTTCCTCCACACGTCTGGTACCACCAGCCGGCCGAAGGGCGTGCCCCTGACTCAACAGAACCTCGCTGCCTCTGTTCAGAACATCAAATCGGTGTACAAGATTTCCGAGTCTGACTCAACCGTACTAGTCCTTCCGTTGTTCCACGTTCACGGATTAATGGCGGGATTGCTATCGTCGCTCATCTCCGGCGCCGCCGTGACTCTTCCGGCAGCCGGTCGATTCTCTGCTTCAACGTTTTGGCCCGATATGCTCACCTATAACGCGACGTGGTACACGGCGGTCCCTACGATTCACCAAATCATACTCGATCGCCACCTCAACAAGCCGGAGGCGGCCTACCCTAAGCTCCGGTTCATCCGGAGCTGCAGCGCGTCCCTTGCACCTTCGATTATGGAGCGCCTAGAAAACTCATTCGGAGCTCCGGTTTTGGAGTCTTACGCGATGACGGAAGCGTCACATCTCATGGCGTCCAATCCGTTGCCGGAGGACGGCGTGCACAAGGCCGGATCGGTAGGGAAGCCGGTGGGCCAAGAAATGGCGATTCTGGACGGGAACGGCGTGCCGCAGTCGGAAGGCGTTAGCGGAGAAGTGTGTATAAGAGGACCAAACGTGACGAAGGGATATAAGAACAATCCAGAAGCCAATAAGGCCGCCTTCTTGTACGGATGGTTCCACACCGGCGATATCGGTTATCTCGACTCCGACGGATACTTGCATCTGGTCGGCCGGATCAAAGAGCTCATCAACCGTGGAGGTACAGTCCAACAGCTCAGATTCGAACTCAAAAAATTAAACGAATCGCCAGTGAACAAAATTCACAATTCAAATTGAAAAAATTGCATCTACAGTCATCTGATCTAATTTGCCCCTTCTTGATCCAGGTGAAAAGATCTCTCCAATTGAAGTAGATTCCGTGCTTCTGTCTCACGTCGACATTGCTCAGGCCGTCGCCTTCGGAGTCCCCGATGAAAAATACGGCGAAGAGGTATGAAAACGTACCGTTGATTATCCTCACATCCGATCGATTCCGATCAATTCACTAGTGATTTGCATTCTAACCGATGCTTCTGCAGATCAATTGCGCTATAATTCCAAGGGAGGGAAGTTCAATCGACGAGGCGGAGGTGATGCGATTTTGCAAGAAAAACCTAGCCTCCTTCAAGGTCCCGAAGAGAGTGTTCATCACCGATTCCCTACCAAAAACTGCTACCGGGAAGATCCAACGCCGAATCGTGGCGGAGCATTTTCTAACTCCGATCGCCGCAGCCGGAGCTTGATCTGATTGGGGGGGATTGAAGATTGGCGTATGCTCCGGTTTTCTGCGATTAATTTCGTCCATTGGAAGTAGAAGCCGTGAGGGCGTTACGTACGTAGGATGTGAGGCAGTGGACAGAGTCACGAGAATGGGCATTGTGGGAATTATGTAGTTTTTATATTTTTTTATTTAATGAAAAAAAAGGAAATACTATAGTTTGAAAATATTTGAAAAACTAAAACTATGGTATGGTGCGTTTGTAATTTTGTTCATGTCATATATAAATAAATTTGTCCTTTTAGCATGACGTATGGGGTCGAGATAAATATTTTAACAAACTTTTCTTTGTTATTTTTAGCCATACGCTCCAGTTATATAAATAAATTTGTTCACTTACAT

mRNA sequence

CTTTATTTTATTTTAGTTCAATTACTTGATCAATATTTATACCTTAAATAAATCTTAAAAATGGTCACCCAATTGACTTAAGGTTAATTTTGATTTGATTTAACAAAAAGGAAAGGCACAACTTCCATTTTAGAAATAGCAAAATGTGAATAAGTTTCCCAAATGTTTAAAGGAAATATCTATACAAGAACTAATTTTGTTTGTTTTCAAAACTTAACCTTGGCCCGTGCAGACTATTTTATTAATTCATCACAAGCTTTAAAATTAGACTTAAACATTTAAAAATAATTAGACTTAAACATTTAAGAATATCTCAACTAACATAGAGTAAGCTCTATAGTAGAAGGATAAATGATAATTTAACAAATTTGTCATTATTCCTTTTAAAGATCATCAATTTGTAAATGTGGAAACGGTATTTAATTGGAAAATATCAGACAACATTTTTAAAGATGGAATTTTTGATCATTGAGTCATCTCATTTTAATGGGATCAAGTCCAAAATAAAGCGATAGGTGGGGCAGGCAAAACAAAGTTCATGAGACAACATATAAGTTGTGGAATCAAAATTTTTATTCATCAATTAGGCATAAAGCTTGTTTTAAAGAGTTCACTTAATTTGGTTTGTTTGGACGGAGTAGAGTGGCGTACAAGAGAGAGTCTAATCAATTTATTTTCGAACAGTCCCTCAAATCAAAATAAAAACAAGTTATGGGCCAAATAATAAAAGAAAAGAAAAGTTCAAAATAGTTTTGAAAACGAATTTTCTAGGAAAATCAAAAATCGAGAAGCCAAATTCTGTTTTGGCTGCTGACACGTGGAGAAAAAAGGAGTCCAGTTTCAACCTTCACGAGTCCTACCTCTCCAAACCACCTAAAAAATCCTATATTAATCCCTCATTTTCTCACCTTTGGAACACGCGTCTCAGTTCTTCTGATATCTGATCAAAGTTTTCCTCAGCCTTTGATTAATTTTTTTTAATTAATTGTTTTGTTTTTTGTTGTTTCATCTGGAAATCCAACTTCCCAGTAGTTTAATCGCGTTCATCAATTTTTGGAAGCGTTTCGATTTCTGAAATTAACAGAAACAGAGAGAGAGAGATGGAGAAGAGTACCTTGAGTGGGTTGTTGAAGAAGGCCGCCTCTGAGTTTCCTCGCCGGCGGGCTCTCTCAGTCTCCGGAAAGTTCGACCTGACACATGAGCGCTTGCAAGAGCTCGTCGAACATGCCGCGTCTCTCCTGGCGGATGCCGGAGTTAACGCCGGCGATGTGGTCGCCCTCACCTTCCATAACACTGTGGAGTTTGTGATCATGTTTCTGGCCGTAATCCGCTGCCGAGCCACGGCTGCGCCATTAAATCCGGCTTACACGGCTGAGGAGTTCGAGTTTTACTTATCGGACTCGGAATCGAAGTTTCTCCTGACGTCAGAAGAAGGGATTCCATCCGCTCAATCCGCCGCATTGAAGCTCAATATCCCTCAAGTAAAAGCAAGCCTGTCTGGCGACGATGAGTTTCTCAATCTTTCTCCGTCAGGAACCGAATCGAAATCGAACATGGCGGAAATCGTAAACGATCCGTCCGATGCGGCGCTCTTCCTCCACACGTCTGGTACCACCAGCCGGCCGAAGGGCGTGCCCCTGACTCAACAGAACCTCGCTGCCTCTGTTCAGAACATCAAATCGGTGTACAAGATTTCCGAGTCTGACTCAACCGTACTAGTCCTTCCGTTGTTCCACGTTCACGGATTAATGGCGGGATTGCTATCGTCGCTCATCTCCGGCGCCGCCGTGACTCTTCCGGCAGCCGGTCGATTCTCTGCTTCAACGTTTTGGCCCGATATGCTCACCTATAACGCGACGTGGTACACGGCGGTCCCTACGATTCACCAAATCATACTCGATCGCCACCTCAACAAGCCGGAGGCGGCCTACCCTAAGCTCCGGTTCATCCGGAGCTGCAGCGCGTCCCTTGCACCTTCGATTATGGAGCGCCTAGAAAACTCATTCGGAGCTCCGGTTTTGGAGTCTTACGCGATGACGGAAGCGTCACATCTCATGGCGTCCAATCCGTTGCCGGAGGACGGCGTGCACAAGGCCGGATCGGTAGGGAAGCCGGTGGGCCAAGAAATGGCGATTCTGGACGGGAACGGCGTGCCGCAGTCGGAAGGCGTTAGCGGAGAAGTGTGTATAAGAGGACCAAACGTGACGAAGGGATATAAGAACAATCCAGAAGCCAATAAGGCCGCCTTCTTGTACGGATGGTTCCACACCGGCGATATCGGTTATCTCGACTCCGACGGATACTTGCATCTGGTCGGCCGGATCAAAGAGCTCATCAACCGTGGAGGTGAAAAGATCTCTCCAATTGAAGTAGATTCCGTGCTTCTGTCTCACGTCGACATTGCTCAGGCCGTCGCCTTCGGAGTCCCCGATGAAAAATACGGCGAAGAGATCAATTGCGCTATAATTCCAAGGGAGGGAAGTTCAATCGACGAGGCGGAGGTGATGCGATTTTGCAAGAAAAACCTAGCCTCCTTCAAGGTCCCGAAGAGAGTGTTCATCACCGATTCCCTACCAAAAACTGCTACCGGGAAGATCCAACGCCGAATCGTGGCGGAGCATTTTCTAACTCCGATCGCCGCAGCCGGAGCTTGATCTGATTGGGGGGGATTGAAGATTGGCGTATGCTCCGGTTTTCTGCGATTAATTTCGTCCATTGGAAGTAGAAGCCGTGAGGGCGTTACGTACGTAGGATGTGAGGCAGTGGACAGAGTCACGAGAATGGGCATTGTGGGAATTATGTAGTTTTTATATTTTTTTATTTAATGAAAAAAAAGGAAATACTATAGTTTGAAAATATTTGAAAAACTAAAACTATGGTATGGTGCGTTTGTAATTTTGTTCATGTCATATATAAATAAATTTGTCCTTTTAGCATGACGTATGGGGTCGAGATAAATATTTTAACAAACTTTTCTTTGTTATTTTTAGCCATACGCTCCAGTTATATAAATAAATTTGTTCACTTACAT

Coding sequence (CDS)

ATGGAGAAGAGTACCTTGAGTGGGTTGTTGAAGAAGGCCGCCTCTGAGTTTCCTCGCCGGCGGGCTCTCTCAGTCTCCGGAAAGTTCGACCTGACACATGAGCGCTTGCAAGAGCTCGTCGAACATGCCGCGTCTCTCCTGGCGGATGCCGGAGTTAACGCCGGCGATGTGGTCGCCCTCACCTTCCATAACACTGTGGAGTTTGTGATCATGTTTCTGGCCGTAATCCGCTGCCGAGCCACGGCTGCGCCATTAAATCCGGCTTACACGGCTGAGGAGTTCGAGTTTTACTTATCGGACTCGGAATCGAAGTTTCTCCTGACGTCAGAAGAAGGGATTCCATCCGCTCAATCCGCCGCATTGAAGCTCAATATCCCTCAAGTAAAAGCAAGCCTGTCTGGCGACGATGAGTTTCTCAATCTTTCTCCGTCAGGAACCGAATCGAAATCGAACATGGCGGAAATCGTAAACGATCCGTCCGATGCGGCGCTCTTCCTCCACACGTCTGGTACCACCAGCCGGCCGAAGGGCGTGCCCCTGACTCAACAGAACCTCGCTGCCTCTGTTCAGAACATCAAATCGGTGTACAAGATTTCCGAGTCTGACTCAACCGTACTAGTCCTTCCGTTGTTCCACGTTCACGGATTAATGGCGGGATTGCTATCGTCGCTCATCTCCGGCGCCGCCGTGACTCTTCCGGCAGCCGGTCGATTCTCTGCTTCAACGTTTTGGCCCGATATGCTCACCTATAACGCGACGTGGTACACGGCGGTCCCTACGATTCACCAAATCATACTCGATCGCCACCTCAACAAGCCGGAGGCGGCCTACCCTAAGCTCCGGTTCATCCGGAGCTGCAGCGCGTCCCTTGCACCTTCGATTATGGAGCGCCTAGAAAACTCATTCGGAGCTCCGGTTTTGGAGTCTTACGCGATGACGGAAGCGTCACATCTCATGGCGTCCAATCCGTTGCCGGAGGACGGCGTGCACAAGGCCGGATCGGTAGGGAAGCCGGTGGGCCAAGAAATGGCGATTCTGGACGGGAACGGCGTGCCGCAGTCGGAAGGCGTTAGCGGAGAAGTGTGTATAAGAGGACCAAACGTGACGAAGGGATATAAGAACAATCCAGAAGCCAATAAGGCCGCCTTCTTGTACGGATGGTTCCACACCGGCGATATCGGTTATCTCGACTCCGACGGATACTTGCATCTGGTCGGCCGGATCAAAGAGCTCATCAACCGTGGAGGTGAAAAGATCTCTCCAATTGAAGTAGATTCCGTGCTTCTGTCTCACGTCGACATTGCTCAGGCCGTCGCCTTCGGAGTCCCCGATGAAAAATACGGCGAAGAGATCAATTGCGCTATAATTCCAAGGGAGGGAAGTTCAATCGACGAGGCGGAGGTGATGCGATTTTGCAAGAAAAACCTAGCCTCCTTCAAGGTCCCGAAGAGAGTGTTCATCACCGATTCCCTACCAAAAACTGCTACCGGGAAGATCCAACGCCGAATCGTGGCGGAGCATTTTCTAACTCCGATCGCCGCAGCCGGAGCTTGA

Protein sequence

MEKSTLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVALTFHNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKFLLTSEEGIPSAQSAALKLNIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPLTQQNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTFWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLENSFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDGNGVPQSEGVSGEVCIRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISPIEVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKVPKRVFITDSLPKTATGKIQRRIVAEHFLTPIAAAGA
Homology
BLAST of MC06g2096 vs. ExPASy Swiss-Prot
Match: M4IQQ7 (Probable CoA ligase CCL9 OS=Humulus lupulus OX=3486 GN=CCL9 PE=2 SV=1)

HSP 1 Score: 806.2 bits (2081), Expect = 2.1e-232
Identity = 409/513 (79.73%), Postives = 452/513 (88.11%), Query Frame = 0

Query: 5   TLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVALTFHN 64
           TL+GLLKKAA+EF  RRA+ VSG+F LTH RLQE+V+HAASLL  +GV  GDVVALTF N
Sbjct: 6   TLTGLLKKAAAEFSDRRAILVSGEFHLTHARLQEIVDHAASLLLASGVGHGDVVALTFPN 65

Query: 65  TVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKFLLTSEEGIPSAQSAALKLN 124
           T+E+++MFLAVIRCRA AAPLN AYTAEEFEFYLSDSESK L+T  +GI +A++AA KLN
Sbjct: 66  TIEYIVMFLAVIRCRAVAAPLNSAYTAEEFEFYLSDSESKLLITPPKGIEAARAAASKLN 125

Query: 125 IPQVKASLSGDDEFLNLSPSGTESKS--NMAEIVNDPSDAALFLHTSGTTSRPKGVPLTQ 184
           I  V A+L G D  + LSPS     S   +AE+ NDPSD +LFLHTSGTTSRPKGVPLTQ
Sbjct: 126 ITHVTATLPGGDGLIALSPSPNNESSLDAVAELTNDPSDVSLFLHTSGTTSRPKGVPLTQ 185

Query: 185 QNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSAST 244
            NLA+SVQNIKSVYK+SESDSTV+VLPLFHVHG++AGLLSSLI+G+AVTLPAAGRFSAST
Sbjct: 186 LNLASSVQNIKSVYKLSESDSTVVVLPLFHVHGMIAGLLSSLIAGSAVTLPAAGRFSAST 245

Query: 245 FWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLENSF 304
           FW DM+  NATWYTAVPTIHQIILDRHLNKPE  YPKLRFIRSCSASLAP+IM RLE SF
Sbjct: 246 FWSDMIACNATWYTAVPTIHQIILDRHLNKPEPTYPKLRFIRSCSASLAPTIMGRLEESF 305

Query: 305 GAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDGNGVPQSEGVSGEVC 364
           GAPVLE+YAMTEA+HLMASNPLPEDG HK GSVGKPVGQEMAILD NG  Q  G SGEVC
Sbjct: 306 GAPVLEAYAMTEAAHLMASNPLPEDGGHKPGSVGKPVGQEMAILDLNGSAQLPGFSGEVC 365

Query: 365 IRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISPI 424
           IRGPNVTKGYKNNPEANKAAF +GWFHTGD+GY D DGYLHLVGRIKELINRGGEKISPI
Sbjct: 366 IRGPNVTKGYKNNPEANKAAFQFGWFHTGDVGYFDEDGYLHLVGRIKELINRGGEKISPI 425

Query: 425 EVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKVP 484
           EVD+VLLSH D+AQAVAFGVPD+KYGEEINCA+IPREGS +DE  V+RFCKKNLA+FKVP
Sbjct: 426 EVDAVLLSHPDLAQAVAFGVPDDKYGEEINCAVIPREGSEVDEDGVLRFCKKNLAAFKVP 485

Query: 485 KRVFITDSLPKTATGKIQRRIVAEHFLTPIAAA 516
           K+VFITDSLPKTATGKIQRRIVAEHFL  I+ A
Sbjct: 486 KKVFITDSLPKTATGKIQRRIVAEHFLAQISTA 518

BLAST of MC06g2096 vs. ExPASy Swiss-Prot
Match: Q9SMT7 (Oxalate--CoA ligase OS=Arabidopsis thaliana OX=3702 GN=AAE3 PE=1 SV=1)

HSP 1 Score: 760.4 bits (1962), Expect = 1.3e-218
Identity = 389/511 (76.13%), Postives = 439/511 (85.91%), Query Frame = 0

Query: 1   MEKSTLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAAS-LLADAGVNAGDVVA 60
           M+  TLSGLL+  A +FP RRALSVSGKF+LTH RL +L+E AAS L++DAG+  GDVVA
Sbjct: 1   MDSDTLSGLLENVAKKFPDRRALSVSGKFNLTHARLHDLIERAASRLVSDAGIKPGDVVA 60

Query: 61  LTFHNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKFLLTSEEGIPSAQSA 120
           LTF NTVEFVIMFLAVIR RATAAPLN AYTAEEFEFYLSDS+SK LLTS+EG   AQ A
Sbjct: 61  LTFPNTVEFVIMFLAVIRARATAAPLNAAYTAEEFEFYLSDSDSKLLLTSKEGNAPAQEA 120

Query: 121 ALKLNIPQVKASLSGDDEFLNLSPSGTESKSNMA-EIVNDPSDAALFLHTSGTTSRPKGV 180
           A KL I  V A+L      L LS + ++S  + A E+VN P D ALFLHTSGTTSRPKGV
Sbjct: 121 ASKLKISHVTATLLDAGSDLVLSVADSDSVVDSATELVNHPDDGALFLHTSGTTSRPKGV 180

Query: 181 PLTQQNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRF 240
           PLTQ NLA+SV+NIK+VYK++ESDSTV+VLPLFHVHGL+AGLLSSL +GAAVTLPAAGRF
Sbjct: 181 PLTQLNLASSVKNIKAVYKLTESDSTVIVLPLFHVHGLLAGLLSSLGAGAAVTLPAAGRF 240

Query: 241 SASTFWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERL 300
           SA+TFWPDM  YNATWYTAVPTIHQIILDRH + PE  YPKLRFIRSCSASLAP I+ RL
Sbjct: 241 SATTFWPDMKKYNATWYTAVPTIHQIILDRHASHPETEYPKLRFIRSCSASLAPVILSRL 300

Query: 301 ENSFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDGNGVPQSEGVS 360
           E +FGAPVLE+YAMTEA+HLM+SNPLPE+G HK GSVGKPVGQEMAIL+  G  Q     
Sbjct: 301 EEAFGAPVLEAYAMTEATHLMSSNPLPEEGPHKPGSVGKPVGQEMAILNEKGEIQEPNNK 360

Query: 361 GEVCIRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEK 420
           GEVCIRGPNVTKGYKNNPEANKA F +GWFHTGDIGY D+DGYLHLVGRIKELINRGGEK
Sbjct: 361 GEVCIRGPNVTKGYKNNPEANKAGFEFGWFHTGDIGYFDTDGYLHLVGRIKELINRGGEK 420

Query: 421 ISPIEVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLAS 480
           ISPIEVD+VLL+H D++Q VAFGVPDEKYGEEINCA+IPREG+++ E ++  FCKKNLA+
Sbjct: 421 ISPIEVDAVLLTHPDVSQGVAFGVPDEKYGEEINCAVIPREGTTVTEEDIKAFCKKNLAA 480

Query: 481 FKVPKRVFITDSLPKTATGKIQRRIVAEHFL 510
           FKVPKRVFITD+LPKTA+GKIQRRIVA+HFL
Sbjct: 481 FKVPKRVFITDNLPKTASGKIQRRIVAQHFL 511

BLAST of MC06g2096 vs. ExPASy Swiss-Prot
Match: O74976 (Oxalate--CoA ligase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=pcs60 PE=1 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 3.2e-119
Identity = 235/513 (45.81%), Postives = 324/513 (63.16%), Query Frame = 0

Query: 1   MEKSTLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVAL 60
           M  +TL   ++  AS    R  ++ S   +L+   L+  +      +A  G+  GD V +
Sbjct: 1   MSFTTLYSAIQGDAS---ARALVAPSLNAELSFSELRIAIMDLQRQIASLGIKVGDPVNI 60

Query: 61  TFHNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKFLLTSEEGIPS---AQ 120
              N +EFV+ F AV   RA   PLN  Y   EFEFY+ D +SK ++  E  + +   A 
Sbjct: 61  AIPNGLEFVVAFYAVSWQRAICGPLNSNYKQSEFEFYIDDLKSKLVIVPEGSVAANTPAV 120

Query: 121 SAALKLNIPQVKASLSGDDEFLNLSP-SGTESKSNMAEIVNDPSDAALFLHTSGTTSRPK 180
            AA KL++   + +       + +    G +  +     +  P D  L LHTSGTT RPK
Sbjct: 121 RAAKKLSVAVAELAWCPKSRLVRIVHFEGAKINAPQPLGLPQPDDVMLVLHTSGTTGRPK 180

Query: 181 GVPLTQQNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAG 240
            VPLT +NL  S+ NI + Y++   D++ +V+PLFHVHGL+ GLLS+L SG    +P   
Sbjct: 181 VVPLTHKNLCRSIHNITTSYRLDPRDTSYVVMPLFHVHGLLCGLLSTLASGGCAVVPP-- 240

Query: 241 RFSASTFWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIME 300
           +FSA +FW + + Y ATWYTAVPTIHQI+L     KP    P++RFIRSCS+ LAP ++ 
Sbjct: 241 KFSAHSFWKEFIQYGATWYTAVPTIHQILLRTPPPKP---LPRIRFIRSCSSPLAPPVLS 300

Query: 301 RLENSFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDGNGVPQSEG 360
           +LE +F APVLE+YAMTEASH M +NPLP   VHK  SVGKP G E+ ILD  G    +G
Sbjct: 301 KLEATFRAPVLEAYAMTEASHQMTTNPLP-PLVHKPHSVGKPFGVELKILDQKGNEMPQG 360

Query: 361 VSGEVCIRGPNVTKGYKNNPEANKAAFLYG-WFHTGDIGYLDSDGYLHLVGRIKELINRG 420
             GE+C+RG NVTKGY NNP ANK++F    +F TGD G LD DGY+ + GRIKEL+NRG
Sbjct: 361 KEGEICVRGINVTKGYLNNPAANKSSFTKDRFFRTGDEGKLDKDGYVFITGRIKELVNRG 420

Query: 421 GEKISPIEVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKN 480
           GEKISP E+D+VL+ H D+++AV F VPDEKYG++I  AI P  G ++   ++  + ++ 
Sbjct: 421 GEKISPAEIDAVLMQHPDVSEAVCFAVPDEKYGQDIQAAINPVAGKTVTPKQLHDYLEQK 480

Query: 481 LASFKVPKRVFITDSLPKTATGKIQRRIVAEHF 509
           +A+FK+PK+ + TD +PKTATGK+QRR+V + F
Sbjct: 481 VAAFKIPKKFYFTDRIPKTATGKVQRRLVCDAF 504

BLAST of MC06g2096 vs. ExPASy Swiss-Prot
Match: P38137 (Oxalate--CoA ligase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PCS60 PE=1 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 6.5e-104
Identity = 226/512 (44.14%), Postives = 301/512 (58.79%), Query Frame = 0

Query: 31  LTHERLQELVEHAASLLAD------AGVNAGDVVALTFHNTVEFVIMFLAVIRCRATAAP 90
           +T+  L  +V H  ++  +        V   D VA++  N +EF++ FL         AP
Sbjct: 32  VTYRDLSHMVGHFQTMFTNPNSPLYGAVFRQDTVAISMRNGLEFIVAFLGATMDAKIGAP 91

Query: 91  LNPAYTAEEFEFYLSDSESKFL--------LTSEEGIPSAQS-----AALKLNIPQVKAS 150
           LNP Y  +EF FYL+D +SK +        L S E + SA +       L  +  + +  
Sbjct: 92  LNPNYKEKEFNFYLNDLKSKAICVPKGTTKLQSSEILKSASTFGCFIVELAFDATRFRVE 151

Query: 151 ---LSGDDEFLNLSPSGTESKSNMAEIVND----------PSDAALFLHTSGTTSRPKGV 210
               S +D +  +         N A+ VN            SD AL LHTSGTTS PK V
Sbjct: 152 YDIYSPEDNYKRV----IYRSLNNAKFVNTNPVKFPGFARSSDVALILHTSGTTSTPKTV 211

Query: 211 PLTQQNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRF 270
           PL   N+  S  NI + YK++  D + +V+PLFHVHGL+  LLS+  +  +V +P    F
Sbjct: 212 PLLHLNIVRSTLNIANTYKLTPLDRSYVVMPLFHVHGLIGVLLSTFRTQGSVVVPDG--F 271

Query: 271 SASTFWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERL 330
               FW   + YN  W++ VPTI  I+L  ++ KP   +P +RFIRSCS++LAP+   +L
Sbjct: 272 HPKLFWDQFVKYNCNWFSCVPTISMIML--NMPKPN-PFPHIRFIRSCSSALAPATFHKL 331

Query: 331 ENSFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDGNGVPQSEGVS 390
           E  F APVLE+YAMTEASH M SN LP  G  K G+VG+P G  + ILD N      G  
Sbjct: 332 EKEFNAPVLEAYAMTEASHQMTSNNLP-PGKRKPGTVGQPQGVTVVILDDNDNVLPPGKV 391

Query: 391 GEVCIRGPNVTKGYKNNPEANKAAFL--YGWFHTGDIGYLDSDGYLHLVGRIKELINRGG 450
           GEV IRG NVT GY NNP+ANK  F     +F TGD GY D +G+L L GRIKELINRGG
Sbjct: 392 GEVSIRGENVTLGYANNPKANKENFTKRENYFRTGDQGYFDPEGFLVLTGRIKELINRGG 451

Query: 451 EKISPIEVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNL 509
           EKISPIE+D ++LSH  I +AVAFGVPD+ YG+ +  AI+ ++G  +   E++ F KK+L
Sbjct: 452 EKISPIELDGIMLSHPKIDEAVAFGVPDDMYGQVVQAAIVLKKGEKMTYEELVNFLKKHL 511

BLAST of MC06g2096 vs. ExPASy Swiss-Prot
Match: Q0K844 (Probable sulfoacetate--CoA ligase OS=Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337) OX=381666 GN=sauT PE=2 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 4.5e-73
Identity = 169/506 (33.40%), Postives = 260/506 (51.38%), Query Frame = 0

Query: 5   TLSGLLKKAASEFP-RRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVALTFH 64
           TL+ L+   A+++P +   LS      LT   L           A AG+ +G  V++   
Sbjct: 12  TLAALIAVRAAQWPDKPYLLSPDSGHALTFGALATDAGTLGRSYAAAGLGSGQTVSVYLP 71

Query: 65  NTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKFLLTSEEGIPSAQSAALKL 124
           N  +   + L  + C     P+N      +  + L+ S+++ + T  +G  + + A  + 
Sbjct: 72  NGEQTARLLLGTMACGLVVNPINLLCQPAQLRYILAHSDTRLVFTWPDGEAAIREALREA 131

Query: 125 NIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPLTQQ 184
            +       + D   L   P+  ++ S +      P   AL ++TSGTT  PKGV LTQ+
Sbjct: 132 GLDVPVLVTAPDANSLPALPATHDAASPLPP--PQPDAPALLMYTSGTTGTPKGVLLTQR 191

Query: 185 NLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTF 244
           NL A+  N+   + +  +D  +  LPL+H++GL+   ++ L+ G +V +P   RFSAS F
Sbjct: 192 NLVANGTNVSREHCLGPADRVLATLPLYHINGLVVTAIAPLVHGGSVVMPM--RFSASAF 251

Query: 245 WPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPK-LRFIRSCSASLAPSIMERLENSF 304
           W D   +  TW   VPTI   +    LN P    P  +RF RS SA+L P      E  F
Sbjct: 252 WQDSARHGCTWLNVVPTIIAYL----LNDPHGQAPAGVRFCRSASAALPPEHHRAFEARF 311

Query: 305 GAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDGNGVPQSEGVSGEVC 364
           G  V+E+  MTE +    SNPL + G  + GS+G+P G    +L  +G P  +G  GE+ 
Sbjct: 312 GIGVIETMGMTETAAPAFSNPL-DPGQRRIGSIGRPSGTRARVLGRDGKPAPDGQVGEIV 371

Query: 365 IRGPNVTKGYKNNPEANKAAFLY-GWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISP 424
           ++G +V  GY   P+  + AF + GW  TGD+GY D+DGY ++ GR KELI +GGE I+P
Sbjct: 372 LQGESVMAGYYKAPDITREAFTHDGWLRTGDLGYRDADGYFYISGRAKELIIKGGENIAP 431

Query: 425 IEVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKV 484
            E+D  LL H  + +A A GVPD  YG+EI   ++ RE +  D+A +   C + L  +K 
Sbjct: 432 REIDEALLRHPGVLEAAAVGVPDPAYGQEIVAYVVMREAARCDDAALRAHCLRELGRYKT 491

Query: 485 PKRVFITDSLPKTATGKIQRRIVAEH 508
           PK       LP+  +GK+QR  +  H
Sbjct: 492 PKEFRFIAELPRGPSGKVQRLKLLNH 508

BLAST of MC06g2096 vs. NCBI nr
Match: XP_022159678.1 (oxalate--CoA ligase-like [Momordica charantia])

HSP 1 Score: 1008 bits (2606), Expect = 0.0
Identity = 517/517 (100.00%), Postives = 517/517 (100.00%), Query Frame = 0

Query: 1   MEKSTLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVAL 60
           MEKSTLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVAL
Sbjct: 1   MEKSTLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVAL 60

Query: 61  TFHNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKFLLTSEEGIPSAQSAA 120
           TFHNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKFLLTSEEGIPSAQSAA
Sbjct: 61  TFHNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKFLLTSEEGIPSAQSAA 120

Query: 121 LKLNIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPL 180
           LKLNIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPL
Sbjct: 121 LKLNIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPL 180

Query: 181 TQQNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA 240
           TQQNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA
Sbjct: 181 TQQNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA 240

Query: 241 STFWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLEN 300
           STFWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLEN
Sbjct: 241 STFWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLEN 300

Query: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDGNGVPQSEGVSGE 360
           SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDGNGVPQSEGVSGE
Sbjct: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDGNGVPQSEGVSGE 360

Query: 361 VCIRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKIS 420
           VCIRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKIS
Sbjct: 361 VCIRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKIS 420

Query: 421 PIEVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFK 480
           PIEVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFK
Sbjct: 421 PIEVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFK 480

Query: 481 VPKRVFITDSLPKTATGKIQRRIVAEHFLTPIAAAGA 517
           VPKRVFITDSLPKTATGKIQRRIVAEHFLTPIAAAGA
Sbjct: 481 VPKRVFITDSLPKTATGKIQRRIVAEHFLTPIAAAGA 517

BLAST of MC06g2096 vs. NCBI nr
Match: XP_038874771.1 (oxalate--CoA ligase-like [Benincasa hispida])

HSP 1 Score: 887 bits (2293), Expect = 0.0
Identity = 447/509 (87.82%), Postives = 477/509 (93.71%), Query Frame = 0

Query: 1   MEKSTLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVAL 60
           ME  TL+GLLKKAASEFPRRRALSVSGKFDLTHERLQELV HAA  L D GV AGDVVAL
Sbjct: 1   MENCTLTGLLKKAASEFPRRRALSVSGKFDLTHERLQELVVHAAGCLVDVGVKAGDVVAL 60

Query: 61  TFHNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKFLLTSEEGIPSAQSAA 120
           TF NTVE+VIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESK L+TS+EGIPSAQ+AA
Sbjct: 61  TFPNTVEYVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLITSKEGIPSAQTAA 120

Query: 121 LKLNIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPL 180
            KLNIPQVKA+LS  +EF++L PS TE+KSN+AEIVNDPSDAALFLHTSGTTSRPKGVPL
Sbjct: 121 TKLNIPQVKANLSSGNEFIDLFPSATETKSNIAEIVNDPSDAALFLHTSGTTSRPKGVPL 180

Query: 181 TQQNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA 240
           TQQNLAASVQNIKSVYK SESDSTVLVLPLFHVHGLMAGLLSSLI+GAAVTLPAAGRFSA
Sbjct: 181 TQQNLAASVQNIKSVYKFSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 240

Query: 241 STFWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLEN 300
           STFW DM+ YNATWYTAVPTIHQIILDRH +KPEA+YPKLRFIRSCSASLAPS++ERLE 
Sbjct: 241 STFWSDMIAYNATWYTAVPTIHQIILDRHFSKPEASYPKLRFIRSCSASLAPSVLERLET 300

Query: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDGNGVPQSEGVSGE 360
           S GAPVLE+YAMTEASHLMASNPLPEDG HKAGSVGKP+GQEMAILD NGV Q EGV GE
Sbjct: 301 SLGAPVLEAYAMTEASHLMASNPLPEDGGHKAGSVGKPIGQEMAILDENGVMQPEGVKGE 360

Query: 361 VCIRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKIS 420
           VCIRG NVTKGYKNNPEANKAAF++GWFHTGD+G+ DSDGYLHLVGRIKELINRGGEKIS
Sbjct: 361 VCIRGANVTKGYKNNPEANKAAFMFGWFHTGDVGFFDSDGYLHLVGRIKELINRGGEKIS 420

Query: 421 PIEVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFK 480
           PIEVD+VLLSH +IAQAVAFGVPDEKYGEEINCAIIPR GSSIDEA+V++FCKKNLASFK
Sbjct: 421 PIEVDAVLLSHANIAQAVAFGVPDEKYGEEINCAIIPRGGSSIDEADVLQFCKKNLASFK 480

Query: 481 VPKRVFITDSLPKTATGKIQRRIVAEHFL 509
           VPK+VFITD LPKTA+GKIQRR VAEHFL
Sbjct: 481 VPKKVFITDYLPKTASGKIQRRFVAEHFL 509

BLAST of MC06g2096 vs. NCBI nr
Match: XP_023534085.1 (oxalate--CoA ligase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 887 bits (2292), Expect = 0.0
Identity = 447/510 (87.65%), Postives = 475/510 (93.14%), Query Frame = 0

Query: 1   MEKSTLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVAL 60
           ME STL+GLLKKAAS+FP RRALSV+GK DLTHERLQEL+EHAAS L DAGVNAGDVVAL
Sbjct: 1   MENSTLTGLLKKAASQFPHRRALSVAGKLDLTHERLQELIEHAASRLVDAGVNAGDVVAL 60

Query: 61  TFHNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKFLLTSEEGIPSAQSAA 120
           TF NTVEFVI FLA+IRCRATAAPLN AYTAEEFEFYLSDSESK LLTS+EGIPSAQ+AA
Sbjct: 61  TFPNTVEFVITFLAIIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGIPSAQTAA 120

Query: 121 LKLNIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPL 180
            KLNIPQV  +L+  D FL+  PS  ES+ NM+EIVNDPSDAALFLHTSGTTSRPKGVPL
Sbjct: 121 SKLNIPQVNVNLASGDAFLDFFPSPNESEVNMSEIVNDPSDAALFLHTSGTTSRPKGVPL 180

Query: 181 TQQNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA 240
           TQQNLAASVQNIKSVYK+SESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA
Sbjct: 181 TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA 240

Query: 241 STFWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLEN 300
           STFW DM+ YNATWYTAVPTIHQIILDRH +KPE +YPKLRFIRSCSASLAPSI+ERLE 
Sbjct: 241 STFWSDMVAYNATWYTAVPTIHQIILDRHFSKPEPSYPKLRFIRSCSASLAPSILERLEK 300

Query: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDGNGVPQSEGVSGE 360
           SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKP+GQEMAILD NGV Q EGV GE
Sbjct: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVIQPEGVKGE 360

Query: 361 VCIRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKIS 420
           VCIRGPNVTKGYKNNPEANKA F +GWFHTGDIG+ DSDGYLHLVGRIKELINRGGEKIS
Sbjct: 361 VCIRGPNVTKGYKNNPEANKATFSFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420

Query: 421 PIEVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFK 480
           PIEVD+VLLSH DIAQAVAFGVPDEKYGEEINCA+IPREGSSIDEAEV+RFCKKNLASFK
Sbjct: 421 PIEVDAVLLSHADIAQAVAFGVPDEKYGEEINCAVIPREGSSIDEAEVLRFCKKNLASFK 480

Query: 481 VPKRVFITDSLPKTATGKIQRRIVAEHFLT 510
           +PK+VFITD LPKTA+GKIQRR VAEHF++
Sbjct: 481 IPKKVFITDFLPKTASGKIQRRFVAEHFVS 510

BLAST of MC06g2096 vs. NCBI nr
Match: XP_022957813.1 (oxalate--CoA ligase-like [Cucurbita moschata])

HSP 1 Score: 886 bits (2290), Expect = 0.0
Identity = 447/510 (87.65%), Postives = 474/510 (92.94%), Query Frame = 0

Query: 1   MEKSTLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVAL 60
           ME STL+GLLKKAAS+FP RRALSV+GK DLTHERLQEL+EHAAS L DAGVNAGDVV L
Sbjct: 1   MENSTLTGLLKKAASQFPHRRALSVAGKLDLTHERLQELIEHAASRLVDAGVNAGDVVTL 60

Query: 61  TFHNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKFLLTSEEGIPSAQSAA 120
           TF NTVEFVI FLA+IRCRATAAPLN AYTAEEFEFYLSDSESK LLTS+EGIPSAQ+AA
Sbjct: 61  TFPNTVEFVITFLAIIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGIPSAQTAA 120

Query: 121 LKLNIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPL 180
            KLNIPQV  +L+  D FL+  PS  ESK NM+EIVNDPSDAALFLHTSGTTSRPKGVPL
Sbjct: 121 SKLNIPQVNVNLASGDAFLDFFPSPNESKVNMSEIVNDPSDAALFLHTSGTTSRPKGVPL 180

Query: 181 TQQNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA 240
           TQQNLAASVQNIKSVYK+SESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA
Sbjct: 181 TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA 240

Query: 241 STFWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLEN 300
           STFW DM+ YNATWYTAVPTIHQIILDRH +KPE +YPKLRFIRSCSASLAPSI+ERLE 
Sbjct: 241 STFWSDMVAYNATWYTAVPTIHQIILDRHFSKPEPSYPKLRFIRSCSASLAPSILERLEK 300

Query: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDGNGVPQSEGVSGE 360
           SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKP+GQEMAILD NGV Q EGV GE
Sbjct: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVIQPEGVKGE 360

Query: 361 VCIRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKIS 420
           VCIRGPNVTKGYKNNPEANKA F +GWFHTGDIG+ DSDGYLHLVGRIKELINRGGEKIS
Sbjct: 361 VCIRGPNVTKGYKNNPEANKATFSFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420

Query: 421 PIEVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFK 480
           PIEVD+VLLSH DIAQAVAFGVPDEKYGEEINCA+IPREGSSIDEAEV+RFCKKNLASFK
Sbjct: 421 PIEVDAVLLSHSDIAQAVAFGVPDEKYGEEINCAVIPREGSSIDEAEVLRFCKKNLASFK 480

Query: 481 VPKRVFITDSLPKTATGKIQRRIVAEHFLT 510
           +PK+VFITD LPKTA+GKIQRR VAEHF++
Sbjct: 481 IPKKVFITDFLPKTASGKIQRRFVAEHFVS 510

BLAST of MC06g2096 vs. NCBI nr
Match: XP_022995200.1 (oxalate--CoA ligase-like [Cucurbita maxima])

HSP 1 Score: 886 bits (2289), Expect = 0.0
Identity = 447/510 (87.65%), Postives = 475/510 (93.14%), Query Frame = 0

Query: 1   MEKSTLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVAL 60
           ME STL+GLLKKAASEFP RRALSV+GK DLTHERLQEL+EHAAS L DAGVNAGDVVAL
Sbjct: 1   MENSTLTGLLKKAASEFPHRRALSVAGKLDLTHERLQELIEHAASRLVDAGVNAGDVVAL 60

Query: 61  TFHNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKFLLTSEEGIPSAQSAA 120
           TF NTVEFVI FLA+IRCRATAAPLN AYTAEEFEFYLSDSESK LLTS+EGIPSAQ+AA
Sbjct: 61  TFPNTVEFVITFLAIIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGIPSAQTAA 120

Query: 121 LKLNIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPL 180
            KLNIPQV  +L+  D FL+  P+ +ESK NMAEIVNDPSDAALFLHTSGTTSRPKGVPL
Sbjct: 121 SKLNIPQVNVNLASGDAFLDFFPAPSESKVNMAEIVNDPSDAALFLHTSGTTSRPKGVPL 180

Query: 181 TQQNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA 240
           TQQNLAASVQNIKSVYK+SESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA
Sbjct: 181 TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA 240

Query: 241 STFWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLEN 300
           STFW DM+ YNATWYTAVPTIHQIILDRH +KPE +YPKLRFIRSCSASLAPSI+ERLE 
Sbjct: 241 STFWSDMVAYNATWYTAVPTIHQIILDRHFSKPEPSYPKLRFIRSCSASLAPSILERLEK 300

Query: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDGNGVPQSEGVSGE 360
           SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKP+GQEMAILD NGV Q EGV GE
Sbjct: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVIQPEGVKGE 360

Query: 361 VCIRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKIS 420
           VCIRGPNVTKGYKNNP+ANKA F +GWFHTGDIG+ DSDGYLHLVGRIKELINRGGEKIS
Sbjct: 361 VCIRGPNVTKGYKNNPDANKATFSFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420

Query: 421 PIEVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFK 480
           PIE+D+VLLSH DIAQAVAFGV DEKYGEEINCAIIPREGSSIDEAEV+RFCKKNLASFK
Sbjct: 421 PIEIDAVLLSHADIAQAVAFGVTDEKYGEEINCAIIPREGSSIDEAEVLRFCKKNLASFK 480

Query: 481 VPKRVFITDSLPKTATGKIQRRIVAEHFLT 510
           +PK+VFITD LPKTA+GKIQRR VAEHF++
Sbjct: 481 IPKKVFITDFLPKTASGKIQRRFVAEHFVS 510

BLAST of MC06g2096 vs. ExPASy TrEMBL
Match: A0A6J1DZI2 (oxalate--CoA ligase-like OS=Momordica charantia OX=3673 GN=LOC111026021 PE=4 SV=1)

HSP 1 Score: 1008 bits (2606), Expect = 0.0
Identity = 517/517 (100.00%), Postives = 517/517 (100.00%), Query Frame = 0

Query: 1   MEKSTLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVAL 60
           MEKSTLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVAL
Sbjct: 1   MEKSTLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVAL 60

Query: 61  TFHNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKFLLTSEEGIPSAQSAA 120
           TFHNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKFLLTSEEGIPSAQSAA
Sbjct: 61  TFHNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKFLLTSEEGIPSAQSAA 120

Query: 121 LKLNIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPL 180
           LKLNIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPL
Sbjct: 121 LKLNIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPL 180

Query: 181 TQQNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA 240
           TQQNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA
Sbjct: 181 TQQNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA 240

Query: 241 STFWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLEN 300
           STFWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLEN
Sbjct: 241 STFWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLEN 300

Query: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDGNGVPQSEGVSGE 360
           SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDGNGVPQSEGVSGE
Sbjct: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDGNGVPQSEGVSGE 360

Query: 361 VCIRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKIS 420
           VCIRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKIS
Sbjct: 361 VCIRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKIS 420

Query: 421 PIEVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFK 480
           PIEVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFK
Sbjct: 421 PIEVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFK 480

Query: 481 VPKRVFITDSLPKTATGKIQRRIVAEHFLTPIAAAGA 517
           VPKRVFITDSLPKTATGKIQRRIVAEHFLTPIAAAGA
Sbjct: 481 VPKRVFITDSLPKTATGKIQRRIVAEHFLTPIAAAGA 517

BLAST of MC06g2096 vs. ExPASy TrEMBL
Match: A0A6J1H1B7 (oxalate--CoA ligase-like OS=Cucurbita moschata OX=3662 GN=LOC111459244 PE=4 SV=1)

HSP 1 Score: 886 bits (2290), Expect = 0.0
Identity = 447/510 (87.65%), Postives = 474/510 (92.94%), Query Frame = 0

Query: 1   MEKSTLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVAL 60
           ME STL+GLLKKAAS+FP RRALSV+GK DLTHERLQEL+EHAAS L DAGVNAGDVV L
Sbjct: 1   MENSTLTGLLKKAASQFPHRRALSVAGKLDLTHERLQELIEHAASRLVDAGVNAGDVVTL 60

Query: 61  TFHNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKFLLTSEEGIPSAQSAA 120
           TF NTVEFVI FLA+IRCRATAAPLN AYTAEEFEFYLSDSESK LLTS+EGIPSAQ+AA
Sbjct: 61  TFPNTVEFVITFLAIIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGIPSAQTAA 120

Query: 121 LKLNIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPL 180
            KLNIPQV  +L+  D FL+  PS  ESK NM+EIVNDPSDAALFLHTSGTTSRPKGVPL
Sbjct: 121 SKLNIPQVNVNLASGDAFLDFFPSPNESKVNMSEIVNDPSDAALFLHTSGTTSRPKGVPL 180

Query: 181 TQQNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA 240
           TQQNLAASVQNIKSVYK+SESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA
Sbjct: 181 TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA 240

Query: 241 STFWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLEN 300
           STFW DM+ YNATWYTAVPTIHQIILDRH +KPE +YPKLRFIRSCSASLAPSI+ERLE 
Sbjct: 241 STFWSDMVAYNATWYTAVPTIHQIILDRHFSKPEPSYPKLRFIRSCSASLAPSILERLEK 300

Query: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDGNGVPQSEGVSGE 360
           SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKP+GQEMAILD NGV Q EGV GE
Sbjct: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVIQPEGVKGE 360

Query: 361 VCIRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKIS 420
           VCIRGPNVTKGYKNNPEANKA F +GWFHTGDIG+ DSDGYLHLVGRIKELINRGGEKIS
Sbjct: 361 VCIRGPNVTKGYKNNPEANKATFSFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420

Query: 421 PIEVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFK 480
           PIEVD+VLLSH DIAQAVAFGVPDEKYGEEINCA+IPREGSSIDEAEV+RFCKKNLASFK
Sbjct: 421 PIEVDAVLLSHSDIAQAVAFGVPDEKYGEEINCAVIPREGSSIDEAEVLRFCKKNLASFK 480

Query: 481 VPKRVFITDSLPKTATGKIQRRIVAEHFLT 510
           +PK+VFITD LPKTA+GKIQRR VAEHF++
Sbjct: 481 IPKKVFITDFLPKTASGKIQRRFVAEHFVS 510

BLAST of MC06g2096 vs. ExPASy TrEMBL
Match: A0A6J1K3G7 (oxalate--CoA ligase-like OS=Cucurbita maxima OX=3661 GN=LOC111490818 PE=4 SV=1)

HSP 1 Score: 886 bits (2289), Expect = 0.0
Identity = 447/510 (87.65%), Postives = 475/510 (93.14%), Query Frame = 0

Query: 1   MEKSTLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVAL 60
           ME STL+GLLKKAASEFP RRALSV+GK DLTHERLQEL+EHAAS L DAGVNAGDVVAL
Sbjct: 1   MENSTLTGLLKKAASEFPHRRALSVAGKLDLTHERLQELIEHAASRLVDAGVNAGDVVAL 60

Query: 61  TFHNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKFLLTSEEGIPSAQSAA 120
           TF NTVEFVI FLA+IRCRATAAPLN AYTAEEFEFYLSDSESK LLTS+EGIPSAQ+AA
Sbjct: 61  TFPNTVEFVITFLAIIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGIPSAQTAA 120

Query: 121 LKLNIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPL 180
            KLNIPQV  +L+  D FL+  P+ +ESK NMAEIVNDPSDAALFLHTSGTTSRPKGVPL
Sbjct: 121 SKLNIPQVNVNLASGDAFLDFFPAPSESKVNMAEIVNDPSDAALFLHTSGTTSRPKGVPL 180

Query: 181 TQQNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA 240
           TQQNLAASVQNIKSVYK+SESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA
Sbjct: 181 TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA 240

Query: 241 STFWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLEN 300
           STFW DM+ YNATWYTAVPTIHQIILDRH +KPE +YPKLRFIRSCSASLAPSI+ERLE 
Sbjct: 241 STFWSDMVAYNATWYTAVPTIHQIILDRHFSKPEPSYPKLRFIRSCSASLAPSILERLEK 300

Query: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDGNGVPQSEGVSGE 360
           SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKP+GQEMAILD NGV Q EGV GE
Sbjct: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVIQPEGVKGE 360

Query: 361 VCIRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKIS 420
           VCIRGPNVTKGYKNNP+ANKA F +GWFHTGDIG+ DSDGYLHLVGRIKELINRGGEKIS
Sbjct: 361 VCIRGPNVTKGYKNNPDANKATFSFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420

Query: 421 PIEVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFK 480
           PIE+D+VLLSH DIAQAVAFGV DEKYGEEINCAIIPREGSSIDEAEV+RFCKKNLASFK
Sbjct: 421 PIEIDAVLLSHADIAQAVAFGVTDEKYGEEINCAIIPREGSSIDEAEVLRFCKKNLASFK 480

Query: 481 VPKRVFITDSLPKTATGKIQRRIVAEHFLT 510
           +PK+VFITD LPKTA+GKIQRR VAEHF++
Sbjct: 481 IPKKVFITDFLPKTASGKIQRRFVAEHFVS 510

BLAST of MC06g2096 vs. ExPASy TrEMBL
Match: A0A5A7THK1 (Oxalate--CoA ligase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002700 PE=4 SV=1)

HSP 1 Score: 885 bits (2288), Expect = 0.0
Identity = 446/509 (87.62%), Postives = 476/509 (93.52%), Query Frame = 0

Query: 1   MEKSTLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVAL 60
           ME  TL+GLLKKAASEFP RRALSVSGKFDLTHERLQEL+EHAA+ L D+GVNAGDVVAL
Sbjct: 1   MENCTLTGLLKKAASEFPHRRALSVSGKFDLTHERLQELIEHAAARLVDSGVNAGDVVAL 60

Query: 61  TFHNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKFLLTSEEGIPSAQSAA 120
           TF NTVE+VIMFLAVIRCRATAAPLN AYTAEEFEFYLSDSESK LLTS+EGI SAQ+AA
Sbjct: 61  TFPNTVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAA 120

Query: 121 LKLNIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPL 180
            KLNIPQVK SLS  DE++ L PS  E  SN+ EIVNDPSDAALFLHTSGTTSRPKGVPL
Sbjct: 121 TKLNIPQVKVSLSSGDEYIELFPSPIEMSSNVVEIVNDPSDAALFLHTSGTTSRPKGVPL 180

Query: 181 TQQNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA 240
           TQQNLAASVQNIKSVYK+SESDSTVLVLPLFHVHGLMAGLLSSLI+GAAVTLPAAGRFSA
Sbjct: 181 TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 240

Query: 241 STFWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLEN 300
           +TFW DM+ YNATWYTAVPTIHQIIL+RH +KPE +YPKLRFIRSCSASLAPSI+ERLE 
Sbjct: 241 TTFWSDMIAYNATWYTAVPTIHQIILERHFSKPEPSYPKLRFIRSCSASLAPSILERLEQ 300

Query: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDGNGVPQSEGVSGE 360
           SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKP+GQEMAILD NG  QSEGV GE
Sbjct: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGVKGE 360

Query: 361 VCIRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKIS 420
           VCIRGPNVTKGYKNNP+ANK+AF++GWFHTGDIG+ DSDGYLHLVGRIKELINRGGEKIS
Sbjct: 361 VCIRGPNVTKGYKNNPDANKSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420

Query: 421 PIEVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFK 480
           PIEVD+VLLSH DI+QAVAFGVPD KYGEEINCAIIPREGSSI+EA+V+ FCKKNLASFK
Sbjct: 421 PIEVDAVLLSHPDISQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLEFCKKNLASFK 480

Query: 481 VPKRVFITDSLPKTATGKIQRRIVAEHFL 509
           VPK+VFITDSLPKTATGKIQRRIVAEHFL
Sbjct: 481 VPKKVFITDSLPKTATGKIQRRIVAEHFL 509

BLAST of MC06g2096 vs. ExPASy TrEMBL
Match: A0A1S3AT78 (oxalate--CoA ligase-like OS=Cucumis melo OX=3656 GN=LOC103482735 PE=4 SV=1)

HSP 1 Score: 885 bits (2287), Expect = 0.0
Identity = 446/509 (87.62%), Postives = 476/509 (93.52%), Query Frame = 0

Query: 1   MEKSTLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVAL 60
           ME  TL+GLLKKAASEFP RRALSVSGKFDLTHERLQEL+EHAA+ L D+GVNAGDVVAL
Sbjct: 1   MENCTLTGLLKKAASEFPHRRALSVSGKFDLTHERLQELIEHAAARLVDSGVNAGDVVAL 60

Query: 61  TFHNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKFLLTSEEGIPSAQSAA 120
           TF NTVE+VIMFLAVIRCRATAAPLN AYTAEEFEFYLSDSESK LLTS+EGI SAQ+AA
Sbjct: 61  TFLNTVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAA 120

Query: 121 LKLNIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPL 180
            KLNIPQVK SLS  DE++ L PS  E  SN+ EIVNDPSDAALFLHTSGTTSRPKGVPL
Sbjct: 121 TKLNIPQVKVSLSSGDEYIELFPSPIEMSSNVVEIVNDPSDAALFLHTSGTTSRPKGVPL 180

Query: 181 TQQNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSA 240
           TQQNLAASVQNIKSVYK+SESDSTVLVLPLFHVHGLMAGLLSSLI+GAAVTLPAAGRFSA
Sbjct: 181 TQQNLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSA 240

Query: 241 STFWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLEN 300
           +TFW DM+ YNATWYTAVPTIHQIIL+RH +KPE +YPKLRFIRSCSASLAPSI+ERLE 
Sbjct: 241 TTFWSDMIAYNATWYTAVPTIHQIILERHFSKPEPSYPKLRFIRSCSASLAPSILERLEQ 300

Query: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDGNGVPQSEGVSGE 360
           SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKP+GQEMAILD NG  QSEGV GE
Sbjct: 301 SFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGVKGE 360

Query: 361 VCIRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKIS 420
           VCIRGPNVTKGYKNNP+ANK+AF++GWFHTGDIG+ DSDGYLHLVGRIKELINRGGEKIS
Sbjct: 361 VCIRGPNVTKGYKNNPDANKSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKIS 420

Query: 421 PIEVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFK 480
           PIEVD+VLLSH DI+QAVAFGVPD KYGEEINCAIIPREGSSI+EA+V+ FCKKNLASFK
Sbjct: 421 PIEVDAVLLSHPDISQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLEFCKKNLASFK 480

Query: 481 VPKRVFITDSLPKTATGKIQRRIVAEHFL 509
           VPK+VFITDSLPKTATGKIQRRIVAEHFL
Sbjct: 481 VPKKVFITDSLPKTATGKIQRRIVAEHFL 509

BLAST of MC06g2096 vs. TAIR 10
Match: AT3G48990.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 760.4 bits (1962), Expect = 9.6e-220
Identity = 389/511 (76.13%), Postives = 439/511 (85.91%), Query Frame = 0

Query: 1   MEKSTLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAAS-LLADAGVNAGDVVA 60
           M+  TLSGLL+  A +FP RRALSVSGKF+LTH RL +L+E AAS L++DAG+  GDVVA
Sbjct: 1   MDSDTLSGLLENVAKKFPDRRALSVSGKFNLTHARLHDLIERAASRLVSDAGIKPGDVVA 60

Query: 61  LTFHNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKFLLTSEEGIPSAQSA 120
           LTF NTVEFVIMFLAVIR RATAAPLN AYTAEEFEFYLSDS+SK LLTS+EG   AQ A
Sbjct: 61  LTFPNTVEFVIMFLAVIRARATAAPLNAAYTAEEFEFYLSDSDSKLLLTSKEGNAPAQEA 120

Query: 121 ALKLNIPQVKASLSGDDEFLNLSPSGTESKSNMA-EIVNDPSDAALFLHTSGTTSRPKGV 180
           A KL I  V A+L      L LS + ++S  + A E+VN P D ALFLHTSGTTSRPKGV
Sbjct: 121 ASKLKISHVTATLLDAGSDLVLSVADSDSVVDSATELVNHPDDGALFLHTSGTTSRPKGV 180

Query: 181 PLTQQNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRF 240
           PLTQ NLA+SV+NIK+VYK++ESDSTV+VLPLFHVHGL+AGLLSSL +GAAVTLPAAGRF
Sbjct: 181 PLTQLNLASSVKNIKAVYKLTESDSTVIVLPLFHVHGLLAGLLSSLGAGAAVTLPAAGRF 240

Query: 241 SASTFWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERL 300
           SA+TFWPDM  YNATWYTAVPTIHQIILDRH + PE  YPKLRFIRSCSASLAP I+ RL
Sbjct: 241 SATTFWPDMKKYNATWYTAVPTIHQIILDRHASHPETEYPKLRFIRSCSASLAPVILSRL 300

Query: 301 ENSFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDGNGVPQSEGVS 360
           E +FGAPVLE+YAMTEA+HLM+SNPLPE+G HK GSVGKPVGQEMAIL+  G  Q     
Sbjct: 301 EEAFGAPVLEAYAMTEATHLMSSNPLPEEGPHKPGSVGKPVGQEMAILNEKGEIQEPNNK 360

Query: 361 GEVCIRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEK 420
           GEVCIRGPNVTKGYKNNPEANKA F +GWFHTGDIGY D+DGYLHLVGRIKELINRGGEK
Sbjct: 361 GEVCIRGPNVTKGYKNNPEANKAGFEFGWFHTGDIGYFDTDGYLHLVGRIKELINRGGEK 420

Query: 421 ISPIEVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLAS 480
           ISPIEVD+VLL+H D++Q VAFGVPDEKYGEEINCA+IPREG+++ E ++  FCKKNLA+
Sbjct: 421 ISPIEVDAVLLTHPDVSQGVAFGVPDEKYGEEINCAVIPREGTTVTEEDIKAFCKKNLAA 480

Query: 481 FKVPKRVFITDSLPKTATGKIQRRIVAEHFL 510
           FKVPKRVFITD+LPKTA+GKIQRRIVA+HFL
Sbjct: 481 FKVPKRVFITDNLPKTASGKIQRRIVAQHFL 511

BLAST of MC06g2096 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 199.1 bits (505), Expect = 8.5e-51
Identity = 161/520 (30.96%), Postives = 246/520 (47.31%), Query Frame = 0

Query: 4   STLSGLLKKAASEFPRRRALSVSGKFD-LTHERLQELVEHAASLLADAGVNAGDVVALTF 63
           ++L   L + +S +P + A++ S   D LT  +L+  V   A      G+   DVV +  
Sbjct: 27  TSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARLAHGFHRLGIRKNDVVLIFA 86

Query: 64  HNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKFLLTSEEGIPSAQSAALK 123
            N+ +F + FLAV          NP YT  E    + DS  K +++  +     +     
Sbjct: 87  PNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKIIISVNQLFDKIKG---- 146

Query: 124 LNIPQVKASLSGDDEFLNLSPSGTESK-------SNMAEIVND-------PSDAALFLHT 183
            ++P V   L G  + + + P G+ SK         ++E V++        SD A  L++
Sbjct: 147 FDLPVV---LLGSKDTVEI-PPGSNSKILSFDNVMELSEPVSEYPFVEIKQSDTAALLYS 206

Query: 184 SGTTSRPKGVPLTQQN-LAASVQNIKSVYKISESDSTVL-VLPLFHVHGLMAGLLSSLIS 243
           SGTT   KGV LT  N +AAS+        + E     L  LP+FHV GL     S L  
Sbjct: 207 SGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLCFLPMFHVFGLAVITYSQLQR 266

Query: 244 GAAVTLPAAGRFSASTFWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSC 303
           G A  L +  RF       ++  +  T    VP +   +  + + K +     L++I S 
Sbjct: 267 GNA--LVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSIVK-KFDLSSLKYIGSG 326

Query: 304 SASLAPSIMERL-ENSFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVG--KPVGQEM 363
           +A L   +ME    N     +++ Y MTE   +++    P  G   +GS G   P  +  
Sbjct: 327 AAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVED-PRLGKRNSGSAGMLAPGVEAQ 386

Query: 364 AILDGNGVPQSEGVSGEVCIRGPNVTKGYKNNPEANKAAF-LYGWFHTGDIGYLDSDGYL 423
            +    G  Q     GE+ +RGPN+ KGY NNP+A K       W HTGD+GY + DG L
Sbjct: 387 IVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWVHTGDLGYFNEDGNL 446

Query: 424 HLVGRIKELINRGGEKISPIEVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSS 483
           ++V RIKELI   G +++P E++ +L+SH DI  AV    PDE+ GE     ++    SS
Sbjct: 447 YVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGEVPIAFVVRSPNSS 506

Query: 484 IDEAEVMRFCKKNLASFKVPKRVFITDSLPKTATGKIQRR 503
           I E ++ +F  K +A +K  +RV     +PK+A GKI RR
Sbjct: 507 ITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRR 534

BLAST of MC06g2096 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 193.7 bits (491), Expect = 3.6e-49
Identity = 142/487 (29.16%), Postives = 237/487 (48.67%), Query Frame = 0

Query: 30  DLTHERLQELVEHAASLLADAGVNAGDVVALTFHNTVEFVIMFLAVIRCRATAAPLNPAY 89
           +LT   L   VE  A  L++ G+  G VV L   N++ F ++ L+V+   A     NP  
Sbjct: 57  NLTFTELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLN 116

Query: 90  TAEEFEFYLSDSESKFLLTSEEGIPSAQSAALKLNI---PQVKASLSGD----DEFLNLS 149
           T+ E    + DS      T+ + +P   +AA KL I    + +    GD     E +   
Sbjct: 117 TSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKE 176

Query: 150 PSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPLTQQNLAASVQNIKSVYKISESD 209
           PSG   K  +     D  D A  L++SGTT   KGV  + +NL A VQ I + +   + +
Sbjct: 177 PSGNRVKERV-----DQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGE 236

Query: 210 ST-VLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTFWPDMLTYNATWYTAVPTI 269
              +  +P+FH++GL A     L  G+  T+    +F        +  Y AT    VP I
Sbjct: 237 QRFICTVPMFHIYGLAAFATGLLAYGS--TIIVLSKFEMHEMMSAIGKYQATSLPLVPPI 296

Query: 270 HQIILDRHLNKPEAAY--PKLRFIRSCSASLAPSIMERLENSF-GAPVLESYAMTEASHL 329
             + +    ++ +A Y    +  +    A L+  + E     +    +L+ Y +TE++ +
Sbjct: 297 -LVAMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGI 356

Query: 330 MASNPLPEDGVHKAGSVGK-PVGQEMAILDG-NGVPQSEGVSGEVCIRGPNVTKGYKNNP 389
            AS    E+   + G+ GK     E  I+D   G       +GE+ ++GP++ KGY +N 
Sbjct: 357 GASTDTVEES-RRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNE 416

Query: 390 EANKAAF-LYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISPIEVDSVLLSHVDIA 449
           EA  +     GW  TGD+ Y+D DG++ +V R+KELI   G +++P E++++LL+H +I 
Sbjct: 417 EATSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEIT 476

Query: 450 QAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKVPKRVFITDSLPKTA 503
            A     PD++ G+     ++ + GSS+ E  +M F  K +A +K  ++V    S+PK  
Sbjct: 477 DAAVIPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNP 534

BLAST of MC06g2096 vs. TAIR 10
Match: AT3G16170.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 191.0 bits (484), Expect = 2.3e-48
Identity = 157/488 (32.17%), Postives = 242/488 (49.59%), Query Frame = 0

Query: 55  GDVVALTFHNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKFLLTSEEGIP 114
           G  + +    + EFV   L        A PL  +Y   E    ++DS+   LL++E+   
Sbjct: 66  GARIGIVAKPSAEFVAGVLGTWFSGGVAVPLALSYPEAELLHVMNDSDISLLLSTEDHSE 125

Query: 115 SAQSAALKLN-----IPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTS 174
           + ++ A K       IP V  S S   E +  +    +S     + ++DP   AL ++TS
Sbjct: 126 TMKTIAAKSGARFHLIPPVVNSTS---ETVACNQFQDDSFEAEGKFLDDP---ALIVYTS 185

Query: 175 GTTSRPKGVPLTQQNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAA 234
           GTT +PKGV  T  ++ + V+ +   ++ + +D  +  LPL HVHGL   L + L + + 
Sbjct: 186 GTTGKPKGVVHTHNSINSQVRMLTEAWEYTSADHFLHCLPLHHVHGLFNALFAPLYARSL 245

Query: 235 VT-LPAAGRFSASTFW----------PDMLTYNATWYTAVPTIHQII------LDRHLNK 294
           V  LP   +FS S  W           +    + T +T VPT++  +      +D+ +  
Sbjct: 246 VEFLP---KFSVSGIWRRWRESYPVNDEKTNDSITVFTGVPTMYTRLIQGYEAMDKEMQD 305

Query: 295 PEA-AYPKLRFIRSCSASLAPSIMERLENSFGAPVLESYAMTEASHLMASNPLPEDGVHK 354
             A A  KLR + S S++L   +M + E+  G  +LE Y MTE    M SNPL   G   
Sbjct: 306 SSAFAARKLRLMMSGSSALPRPVMHQWESITGHRLLERYGMTEFVMAM-SNPL--RGARN 365

Query: 355 AGSVGKPV-GQEMAIL----DGNGVPQSEGVSGEVCIRGPNVTKGYKNNPEANKAAFLY- 414
           AG+VGKP+ G E  I     D NGV       GE+C++ P++ K Y N PE  K +F   
Sbjct: 366 AGTVGKPLPGVEAKIKEDENDANGV-------GEICVKSPSLFKEYWNLPEVTKESFTED 425

Query: 415 GWFHTGDIGYLDSDGYLHLVGR-IKELINRGGEKISPIEVDSVLLSHVDIAQAVAFGVPD 474
           G+F TGD G +D DGY  ++GR   +++  GG K+S +E++S LL H  +A+    G+ D
Sbjct: 426 GYFKTGDAGRVDEDGYYVILGRNSADIMKVGGYKLSALEIESTLLEHPTVAECCVLGLTD 485

Query: 475 EKYGEEINCAIIP-------REGSS---IDEAEVMRFCKKNLASFKVPKRVFITDSLPKT 503
             YGE +   II        RE  S   I   E+  + K  LA +K+P R+ I +SLP+ 
Sbjct: 486 NDYGEAVTAIIIAESAAKKRREDESKPVITLEELCGWAKDKLAPYKLPTRLLIWESLPRN 534

BLAST of MC06g2096 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 189.9 bits (481), Expect = 5.2e-48
Identity = 151/494 (30.57%), Postives = 233/494 (47.17%), Query Frame = 0

Query: 32  THERLQELVEHAASLLADAGVNAGDVVALTFHNTVEFVIMFLAVIRCRATAAPLNPAYTA 91
           T+     +    AS L   G+  GDV+ +   N+ EFV  F+      A +   NP YT+
Sbjct: 77  TYGETHLICRRVASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTS 136

Query: 92  EEFEFYLSDSESKFLLTSEEGIPSAQSAALKLNIPQVKASLSGDDEFLNLSPSGTESKSN 151
           +E    L  S +K ++T  + +   ++  L  N+  +       +  L  S   T+ ++N
Sbjct: 137 QELYKQLKSSGAKLIITHSQYVDKLKN--LGENLTLITTDEPTPENCLPFSTLITDDETN 196

Query: 152 MAEIVND--PSDAALFLHTSGTTSRPKGVPLTQQNLAASVQ-----NIKSVYKISESDST 211
             +   D    DAA    +SGTT  PKGV LT ++L  SV      +  ++Y +  +D  
Sbjct: 197 PFQETVDIGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLY-LKSNDVI 256

Query: 212 VLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTFWPDMLTYNATWYTAVPTIHQI 271
           + VLPLFH++ L + LL+SL SGA V L    +F        +  +  T    VP +  I
Sbjct: 257 LCVLPLFHIYSLNSVLLNSLRSGATVLL--MHKFEIGALLDLIQRHRVTIAALVPPL-VI 316

Query: 272 ILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLENSFGAPVL-ESYAMTEASHLM---- 331
            L ++          +RF+ S +A L   + + L       +L + Y MTEA  ++    
Sbjct: 317 ALAKNPTVNSYDLSSVRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSL 376

Query: 332 --ASNPLPEDGVHKAGSVG--------KPVGQEMAILDGNGVPQSEGVSGEVCIRGPNVT 391
             A  P+P     K+GS G        K V  E  +  G   P      GE+CIRG  + 
Sbjct: 377 GFAKEPIPT----KSGSCGTVVRNAELKVVHLETRLSLGYNQP------GEICIRGQQIM 436

Query: 392 KGYKNNPEANKAAF-LYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISPIEVDSVL 451
           K Y N+PEA  A     GW HTGDIGY+D D  + +V R+KE+I   G ++ P E++S+L
Sbjct: 437 KEYLNDPEATSATIDEEGWLHTGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLL 496

Query: 452 LSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKVPKRVFIT 503
           ++H  IA A      DE  GE     ++   G+ I E +V  +  K +  +K   +VF  
Sbjct: 497 INHHSIADAAVVPQNDEVAGEVPVAFVVRSNGNDITEEDVKEYVAKQVVFYKRLHKVFFV 554

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
M4IQQ72.1e-23279.73Probable CoA ligase CCL9 OS=Humulus lupulus OX=3486 GN=CCL9 PE=2 SV=1[more]
Q9SMT71.3e-21876.13Oxalate--CoA ligase OS=Arabidopsis thaliana OX=3702 GN=AAE3 PE=1 SV=1[more]
O749763.2e-11945.81Oxalate--CoA ligase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=28... [more]
P381376.5e-10444.14Oxalate--CoA ligase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=... [more]
Q0K8444.5e-7333.40Probable sulfoacetate--CoA ligase OS=Cupriavidus necator (strain ATCC 17699 / DS... [more]
Match NameE-valueIdentityDescription
XP_022159678.10.0100.00oxalate--CoA ligase-like [Momordica charantia][more]
XP_038874771.10.087.82oxalate--CoA ligase-like [Benincasa hispida][more]
XP_023534085.10.087.65oxalate--CoA ligase-like [Cucurbita pepo subsp. pepo][more]
XP_022957813.10.087.65oxalate--CoA ligase-like [Cucurbita moschata][more]
XP_022995200.10.087.65oxalate--CoA ligase-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1DZI20.0100.00oxalate--CoA ligase-like OS=Momordica charantia OX=3673 GN=LOC111026021 PE=4 SV=... [more]
A0A6J1H1B70.087.65oxalate--CoA ligase-like OS=Cucurbita moschata OX=3662 GN=LOC111459244 PE=4 SV=1[more]
A0A6J1K3G70.087.65oxalate--CoA ligase-like OS=Cucurbita maxima OX=3661 GN=LOC111490818 PE=4 SV=1[more]
A0A5A7THK10.087.62Oxalate--CoA ligase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
A0A1S3AT780.087.62oxalate--CoA ligase-like OS=Cucumis melo OX=3656 GN=LOC103482735 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G48990.19.6e-22076.13AMP-dependent synthetase and ligase family protein [more]
AT4G05160.18.5e-5130.96AMP-dependent synthetase and ligase family protein [more]
AT1G20510.13.6e-4929.16OPC-8:0 CoA ligase1 [more]
AT3G16170.12.3e-4832.17AMP-dependent synthetase and ligase family protein [more]
AT1G65060.15.2e-4830.574-coumarate:CoA ligase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 4..407
e-value: 1.3E-105
score: 355.3
NoneNo IPR availableGENE3D3.30.300.30coord: 408..509
e-value: 3.2E-28
score: 100.3
NoneNo IPR availablePANTHERPTHR43201ACYL-COA SYNTHETASEcoord: 3..515
NoneNo IPR availablePANTHERPTHR43201:SF17OXALATE--COA LIGASE-LIKEcoord: 3..515
NoneNo IPR availableCDDcd05926FACL_fum10p_likecoord: 18..506
e-value: 0.0
score: 734.888
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 5..510
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 11..414
e-value: 7.1E-96
score: 321.4
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 423..498
e-value: 9.2E-17
score: 61.8
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 165..176

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g2096.1MC06g2096.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006631 fatty acid metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0031956 medium-chain fatty acid-CoA ligase activity