MC06g1384 (gene) Bitter gourd (Dali-11) v1

BLAST of MC06g1384 vs. ExPASy Swiss-Prot
Match: Q9FK05 (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3702 GN=PME61 PE=2 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 2.1e-207
Identity = 365/588 (62.07%), Postives = 452/588 (76.87%), Query Frame = 0

Query: 15  PANFELSNSNSSISSPNRRTRTRVVLLSLLSILLIMASAISAVVFVRSHIGRGGESGLGL 74
           P+N    +  +S+    ++T+T+++L +L    +++   +   +F        G++   L
Sbjct: 11  PSNPNQKDPATSLPELQKKTKTKLILFTL---AVLVVGVVCFGIFAGIRAVDSGKTEPKL 70

Query: 75  AHKPSQAISRACSRTLYPAVCVSSLVEFPGALAADVHDLVHISLNVTLQRLTKALYSSSQ 134
             KP+QAISR CS++LYP +C+ +L++FPG+L AD ++L+HIS N TLQ+ +KALY+SS 
Sbjct: 71  TRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSST 130

Query: 135 IPVSQISDPLLLSAFADCIELLDDAVDAFSRSLFSTAA-----SNHDVLTWLSAALTYHD 194
           I  +Q+  P + SA+  C+ELLDD+VDA +R+L S        S+ DV+TWLS+A+T HD
Sbjct: 131 ITYTQM-PPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTNHD 190

Query: 195 TCTEGLADV--SAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPVQNRRRLM 254
           TCT+G  ++    G+VKDQ+   + DLS+++SN LAIF+   G   + SGVPV N R+L+
Sbjct: 191 TCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFA---GKVKDLSGVPVVNNRKLL 250

Query: 255 EAEWPQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAIAAAPSSS 314
             E       EE P W+ R+DR+LLG+P SAIQADI V+KDG+G +KT+AEAI  AP  S
Sbjct: 251 GTE-----ETEELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHS 310

Query: 315 GRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAAS 374
            RR VIYVKAG+Y EENLKVGRKKTNLMF+GDGKG TVI+GGKS+ D +TTF TATFAA+
Sbjct: 311 SRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAAT 370

Query: 375 GTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQFYRECDI 434
           G G I+RDMTFEN AGP +HQAVALRV  DHAVV+RCNIIGYQD LYVHSNRQF+REC+I
Sbjct: 371 GAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEI 430

Query: 435 YGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKILAASDL 494
           YGTVDFIFGNA V+ QSCNIYARKPM  QK TITAQ+RKDPNQNTGISIHACK+LA  DL
Sbjct: 431 YGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDL 490

Query: 495 EGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPG 554
           E SKGSYPT+LGRPWKLYSRVVYM+S MGDHI PRGWLEW G FALD+LYYGEYMN G G
Sbjct: 491 EASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLG 550

Query: 555 AAVGQRVKWPGYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFSAGLS 596
           + +GQRVKWPGY VITSTVEA KFTV QFI GS+WLPSTGVSF +GLS
Sbjct: 551 SGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

BLAST of MC06g1384 vs. ExPASy Swiss-Prot
Match: Q9M3B0 (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3702 GN=PME34 PE=2 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 8.6e-201
Identity = 368/593 (62.06%), Postives = 453/593 (76.39%), Query Frame = 0

Query: 8   STSSGDTPANFELSNSNSSISSPNRRTRTRVVLLSL-LSILLIMASAISAVVFVRSHIGR 67
           +TS+   P   ++S S     + NRR++ ++V+ S+ L+I LI+A+AI A V  R  +  
Sbjct: 16  TTSTTTAPILNQVSTSEQP-ENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKL-- 75

Query: 68  GGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALAA-DVHDLVHISLNVTLQRL 127
             +S  GLA KPSQAIS+AC  T +P +CV SL++FPG+LAA    DL+H+++N+TL   
Sbjct: 76  -NQSVPGLARKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHF 135

Query: 128 TKALYSSSQIPVSQISDPLLLSAFADCIELLDDAVDAFSRSLFSTAASN---HDVLTWLS 187
           + ALYSS+ +    +  P   SA+  C+ELLDD+VDA SR+L S  +S+    DV TWLS
Sbjct: 136 SHALYSSASLSFVDM-PPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLS 195

Query: 188 AALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPVQN 247
           AALT HDTCTEG   V  G VKD MTA L +LS+L+SN LAIFS    G  +F+GVP+QN
Sbjct: 196 AALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDG-DDFAGVPIQN 255

Query: 248 RRRLMEAEWPQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAIAA 307
           RR L       E   E+FP W+  K+R++L  P+S IQADI+V+KDG G  KT++EAI  
Sbjct: 256 RRLL-----GVEEREEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKK 315

Query: 308 APSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTA 367
           AP +S RR +IYVKAG+Y E NLKVGRKK NLMFVGDGKG TVISGGKS+FD +TTF TA
Sbjct: 316 APQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTA 375

Query: 368 TFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQFY 427
           +FAA+G G I RD+TFEN AGP +HQAVALR+ ADHAV++RCNIIGYQDTLYVHSNRQF+
Sbjct: 376 SFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFF 435

Query: 428 RECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKIL 487
           RECDIYGTVDFIFGNA VV Q+C+IYARKPM  QKNTITAQ+RKDPNQNTGISIHA ++L
Sbjct: 436 RECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVL 495

Query: 488 AASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYM 547
           AASDL+ + GS  T+LGRPWKL+SR VYM+S +G H+H RGWLEW  +FALDTLYYGEY+
Sbjct: 496 AASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYL 555

Query: 548 NSGPGAAVGQRVKWPGYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFSAGLS 596
           NSGPG+ +GQRV WPGYRVI ST EA +FTV +FIYGS+WLPSTGVSF AGLS
Sbjct: 556 NSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597

BLAST of MC06g1384 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 2.7e-122
Identity = 256/589 (43.46%), Postives = 363/589 (61.63%), Query Frame = 0

Query: 19  ELSNSNSSISSPNRRTRTRVVLLSLLSILLIMASAISAVVFVRSHIGRGGESGLGLAHKP 78
           +LS S+S+ +  N   + + + L+L + LL++A+ I  V  V S     G++G    ++P
Sbjct: 10  KLSESSSNQNISNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSR-KNSGDNG----NEP 69

Query: 79  SQAISR-ACSRTLYPAVCVSSLVEFPGA--LAADVHDLVHISLNVTLQRLTKALYSSSQI 138
             AI + +CS T YP +C S++   P A        D++ +SLN+T   +    +   ++
Sbjct: 70  HHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKL 129

Query: 139 PVSQISDPLLLSAFADCIELLDDAVDAFSRSL-------FSTAASNH--DVLTWLSAALT 198
                       A  DC+E +D+ +D   +++          + S H  D+ T +SAA+T
Sbjct: 130 LKRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMT 189

Query: 199 YHDTCTEGLADVSAGQ-VKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPVQNRRR 258
              TC +G +   A + V+D ++     + ++ SN+LA+         +   +   N R+
Sbjct: 190 NQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNM--TDTDMMIMRTSNNRK 249

Query: 259 LMEAEWPQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAIAAAPS 318
           L E    + +  + +P W+S  DR+LL S  S++  + VVA DG+G +KTVA A+AAAP 
Sbjct: 250 LTE----ETSTVDGWPAWLSPGDRRLLQS--SSVTPNAVVAADGSGNFKTVAAAVAAAPQ 309

Query: 319 SSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFA 378
              +R +I +KAG Y  EN++V +K  N+MF+GDG+  T+I+G ++V D  TTF++AT A
Sbjct: 310 GGTKRYIIRIKAGVY-RENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAA 369

Query: 379 ASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQFYREC 438
             G G + RD+TF+NTAGP +HQAVALRV AD +  + C+++ YQDTLYVHSNRQF+  C
Sbjct: 370 VVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNC 429

Query: 439 DIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKILAAS 498
            I GTVDFIFGNA  V Q+C+I+ARKP  GQKN +TAQ R DPNQNTGI I   +I A S
Sbjct: 430 LIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATS 489

Query: 499 DLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSG 558
           DL+  +GS+PT+LGRPWK YSR V M SS+ D IHP GW EW G+FAL+TL+YGE+ NSG
Sbjct: 490 DLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSG 549

Query: 559 PGAAVGQRVKWPGYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFSAGL 595
            GA    RVKW G+RVITS  EA  FT G FI GS+WL STG  FS GL
Sbjct: 550 AGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of MC06g1384 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 3.6e-122
Identity = 253/589 (42.95%), Postives = 365/589 (61.97%), Query Frame = 0

Query: 19  ELSNSNSSISSPNRRTRTRVVLLSLLSILLIMASAISAVVFVRSHIGRGGESGLGLAHKP 78
           +LS S+++ +  N   + + + L+L + LL++A+ I  V  V S     G++G    ++P
Sbjct: 10  KLSESSTNQNISNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSR-KNSGDNG----NEP 69

Query: 79  SQAISR-ACSRTLYPAVCVSSLVEFPGA--LAADVHDLVHISLNVTLQRLTKALYSSSQI 138
             AI + +CS T YP +C S++   P A        D++ +SLN+T   +    +   ++
Sbjct: 70  HHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKL 129

Query: 139 PVSQISDPLLLSAFADCIELLDDAVDAFSRSL-------FSTAASNH--DVLTWLSAALT 198
                       A  DC+E +D+ +D   +++          + S H  D+ T +SAA+T
Sbjct: 130 LKRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMT 189

Query: 199 YHDTCTEGLADVSAGQ-VKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPVQNRRR 258
              TC +G +   A + V+D ++     + ++ SN+LA+         +   +   N R+
Sbjct: 190 NQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNM--TDTDMMIMRTSNNRK 249

Query: 259 LMEAEWPQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAIAAAPS 318
           L+E    + +  + +P W+S  DR+LL S  S++  ++VVA DG+G +KTVA ++AAAP 
Sbjct: 250 LIE----ETSTVDGWPAWLSTGDRRLLQS--SSVTPNVVVAADGSGNFKTVAASVAAAPQ 309

Query: 319 SSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFA 378
              +R +I +KAG Y  EN++V +K  N+MF+GDG+  T+I+G ++V D  TTF++AT A
Sbjct: 310 GGTKRYIIRIKAGVY-RENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVA 369

Query: 379 ASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQFYREC 438
             G G + RD+TF+NTAGP +HQAVALRV AD +  + C+++ YQDTLYVHSNRQF+  C
Sbjct: 370 VVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNC 429

Query: 439 DIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKILAAS 498
            I GTVDFIFGNA  V Q+C+I+ARKP  GQKN +TAQ R DPNQNTGI I   +I A S
Sbjct: 430 LIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATS 489

Query: 499 DLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSG 558
           DL+  +GS+PT+LGRPWK YSR V M SS+ D IHP GW EW G+FAL+TL+YGE+ N+G
Sbjct: 490 DLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAG 549

Query: 559 PGAAVGQRVKWPGYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFSAGL 595
            GA    RVKW G+RVITS  EA  FT G FI GS+WL STG  FS GL
Sbjct: 550 AGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of MC06g1384 vs. ExPASy Swiss-Prot
Match: Q43143 (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 2.0e-120
Identity = 245/577 (42.46%), Postives = 352/577 (61.01%), Query Frame = 0

Query: 35  RTRVVLLSLLSILLIMASAISAVVFVRSHIGRGGESG--LGLAHKPSQAISRACSRTLYP 94
           R + + L++++ +L++A+ I  V  V+SH     +    + ++      +  ACS TL+P
Sbjct: 17  RKKKIYLAIVASVLLVAAVIGVVAGVKSHSKNSDDHADIMAISSSAHAIVKSACSNTLHP 76

Query: 95  AVCVSSLVEFP--GALAADVHDLVHISLNVTLQRLTKALYSSSQ-IPVSQISDPLLLSAF 154
            +C S++V             D++ +SLN+T++ + +  Y+  + I   +   P    A 
Sbjct: 77  ELCYSAIVNVSDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKELIKTRKGLTPREKVAL 136

Query: 155 ADCIELLDDAVDAFSRSL---------FSTAASNHDVLTWLSAALTYHDTCTEGLA-DVS 214
            DC+E +D+ +D    ++          S      D+ T +S+A+T  +TC +G + D +
Sbjct: 137 HDCLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISSAITNQETCLDGFSHDEA 196

Query: 215 AGQVKDQMTAKLADLSQLISNSLAIFSGFGGG--TGEFSGVPVQNRRRLMEAEWPQENGG 274
             +V+  +      + ++ SN+LA+           E       N R+L+E     +NG 
Sbjct: 197 DKKVRKVLLKGQKHVEKMCSNALAMICNMTDTDIANEMKLSAPANNRKLVE-----DNG- 256

Query: 275 EEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAIAAAPSSSGRRTVIYVKA 334
            E+PEW+S  DR+LL S  S +  D+VVA DG+G YKTV+EA+  AP  S +R VI +KA
Sbjct: 257 -EWPEWLSAGDRRLLQS--STVTPDVVVAADGSGDYKTVSEAVRKAPEKSSKRYVIRIKA 316

Query: 335 GKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAASGTGIILRDMT 394
           G Y  EN+ V +KKTN+MF+GDGK  T+I+  ++V D  TTF +AT       ++ RD+T
Sbjct: 317 GVY-RENVDVPKKKTNIMFMGDGKSNTIITASRNVQDGSTTFHSATVVRVAGKVLARDIT 376

Query: 395 FENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQFYRECDIYGTVDFIFGN 454
           F+NTAG  +HQAVAL V +D +  +RC+++ YQDTLYVHSNRQF+ +C + GTVDFIFGN
Sbjct: 377 FQNTAGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVHSNRQFFVQCLVAGTVDFIFGN 436

Query: 455 AVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKILAASDLEGSKGSYPTF 514
              VFQ C+I+AR+P  GQKN +TAQ R DPNQNTGI I  C+I A SDL   + S+PT+
Sbjct: 437 GAAVFQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRPVQKSFPTY 496

Query: 515 LGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRVKWP 574
           LGRPWK YSR V M SS+ D I P GW EW G+FALDTL+YGEY N+G GA    RVKW 
Sbjct: 497 LGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTLFYGEYANTGAGAPTSGRVKWK 556

Query: 575 GYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFSAGL 595
           G++VITS+ EA  +T G+FI G +WL STG  FS GL
Sbjct: 557 GHKVITSSTEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583

BLAST of MC06g1384 vs. NCBI nr
Match: XP_022158049.1 (probable pectinesterase/pectinesterase inhibitor 61 [Momordica charantia])

HSP 1 Score: 1173 bits (3035), Expect = 0.0
Identity = 597/597 (100.00%), Postives = 597/597 (100.00%), Query Frame = 0

Query: 1   MNYGKLHSTSSGDTPANFELSNSNSSISSPNRRTRTRVVLLSLLSILLIMASAISAVVFV 60
           MNYGKLHSTSSGDTPANFELSNSNSSISSPNRRTRTRVVLLSLLSILLIMASAISAVVFV
Sbjct: 1   MNYGKLHSTSSGDTPANFELSNSNSSISSPNRRTRTRVVLLSLLSILLIMASAISAVVFV 60

Query: 61  RSHIGRGGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALAADVHDLVHISLNV 120
           RSHIGRGGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALAADVHDLVHISLNV
Sbjct: 61  RSHIGRGGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALAADVHDLVHISLNV 120

Query: 121 TLQRLTKALYSSSQIPVSQISDPLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDVLTW 180
           TLQRLTKALYSSSQIPVSQISDPLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDVLTW
Sbjct: 121 TLQRLTKALYSSSQIPVSQISDPLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDVLTW 180

Query: 181 LSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPV 240
           LSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPV
Sbjct: 181 LSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPV 240

Query: 241 QNRRRLMEAEWPQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAI 300
           QNRRRLMEAEWPQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAI
Sbjct: 241 QNRRRLMEAEWPQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAI 300

Query: 301 AAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFR 360
           AAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFR
Sbjct: 301 AAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFR 360

Query: 361 TATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQ 420
           TATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQ
Sbjct: 361 TATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQ 420

Query: 421 FYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACK 480
           FYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACK
Sbjct: 421 FYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACK 480

Query: 481 ILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGE 540
           ILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGE
Sbjct: 481 ILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGE 540

Query: 541 YMNSGPGAAVGQRVKWPGYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFSAGLSAS 597
           YMNSGPGAAVGQRVKWPGYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFSAGLSAS
Sbjct: 541 YMNSGPGAAVGQRVKWPGYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFSAGLSAS 597

BLAST of MC06g1384 vs. NCBI nr
Match: XP_022957644.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita moschata])

HSP 1 Score: 922 bits (2382), Expect = 0.0
Identity = 477/602 (79.24%), Postives = 528/602 (87.71%), Query Frame = 0

Query: 2   NYGKLHSTSSGDTPANFELSNSNSSISSPNRRTRTRVVLLSLLSILLIMASAISAVVFVR 61
           NY KLHS++SGDTPA F++S++ +  SS   R RTR++ +SL+SILLI ASA+SA V + 
Sbjct: 3   NYNKLHSSASGDTPATFQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAVLLH 62

Query: 62  SHIGRGGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALAADVHDLVHISLNVT 121
           S      +S     +KP+QAIS ACS TLYP++C SSL+ FPG+L+ADVH LVHISLN+T
Sbjct: 63  S------QSSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNLT 122

Query: 122 LQRLTKALYSSSQIPVSQISD-PLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDVLTW 181
           LQ LTKALY+SSQIPV QIS+ PL  SA+ DCIELL+DAVDAFS SLFST+ASNHD++TW
Sbjct: 123 LQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTW 182

Query: 182 LSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPV 241
           LSAALTYHDTC++G ADV    VKDQM A+L+DLS++ISNSLAIFSG+G       G+PV
Sbjct: 183 LSAALTYHDTCSDGFADVD-DPVKDQMAARLSDLSEMISNSLAIFSGYG------EGLPV 242

Query: 242 QNRRRLMEA--EWPQENGGE----EFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYK 301
           QNRRRLME+  EW  ENG      EFP WVS KDR+LL +PISAIQADIVVAKDG+GK+K
Sbjct: 243 QNRRRLMESDREWAAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFK 302

Query: 302 TVAEAIAAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFD 361
           TVAEAI AAPSSSGRR VIY+KAGKY EENLKVGRKKTNLMFVGDGKGITVISGGKSVFD
Sbjct: 303 TVAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFD 362

Query: 362 KVTTFRTATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLY 421
           KVTTFRTATFA SGTGIILRDMTFENTAGP +HQAVALRVSADHAVV+RCNIIGYQDTLY
Sbjct: 363 KVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRVSADHAVVYRCNIIGYQDTLY 422

Query: 422 VHSNRQFYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGI 481
           VHSNRQF+RECDIYGTVDFIFGNAVVVFQSCNIYARKPMP QKNTITAQ+RKDPNQNTGI
Sbjct: 423 VHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQNTGI 482

Query: 482 SIHACKILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD 541
           SIHACKILA +DLE SKGS+PTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD
Sbjct: 483 SIHACKILATTDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD 542

Query: 542 TLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFSAGL 596
           TLYYGEYMNSGPGAA GQRV WPGYRVITST+EA KFTVGQFIYGS+WLPSTGVSFSAGL
Sbjct: 543 TLYYGEYMNSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFSAGL 591

BLAST of MC06g1384 vs. NCBI nr
Match: XP_023533011.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 917 bits (2371), Expect = 0.0
Identity = 473/601 (78.70%), Postives = 524/601 (87.19%), Query Frame = 0

Query: 2   NYGKLHSTSSGDTPANFELSNSNSSISSPNRRTRTRVVLLSLLSILLIMASAISAVVFVR 61
           NY KLHS+ SGDTPA F++S++ +  SS   R RTR++ +SL+SILLI ASA+SA V + 
Sbjct: 3   NYNKLHSSGSGDTPATFQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAVLIH 62

Query: 62  SHIGRGGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALAADVHDLVHISLNVT 121
           ++      +      KP+QAIS ACS TLYP++C SSL+ FPG+L+ADVH LVHISLN+T
Sbjct: 63  TNTSHFPHN------KPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHHLVHISLNLT 122

Query: 122 LQRLTKALYSSSQIPVSQISD-PLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDVLTW 181
           LQ LTKALY+SSQIPV QIS+ PL  SA+ DCIELL+DAVDAFS SLFST+ASNHD++TW
Sbjct: 123 LQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTW 182

Query: 182 LSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPV 241
           LSAALTYHDTC++G ADV    VKDQM A+L+DLS++ISNSLAIFSG+G       G+PV
Sbjct: 183 LSAALTYHDTCSDGFADVD-DPVKDQMAARLSDLSEMISNSLAIFSGYG------QGLPV 242

Query: 242 QNRRRLMEA--EWPQENGGE----EFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYK 301
           QNRRRLME+  EW  ENG      EFP WVS KDR+LL +PISAIQADIVVAKDG+GK+ 
Sbjct: 243 QNRRRLMESDSEWAAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFT 302

Query: 302 TVAEAIAAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFD 361
           TVAEAI AAPSSSGRR VIY+KAGKY EENLKVGRKKTNLMFVGDGKGITVISGGKSVFD
Sbjct: 303 TVAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFD 362

Query: 362 KVTTFRTATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLY 421
           KVTTFRTATFA SGTGIILRDMTFENTAGP +HQAVALRVSADHAVV+RCNIIGYQDTLY
Sbjct: 363 KVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRVSADHAVVYRCNIIGYQDTLY 422

Query: 422 VHSNRQFYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGI 481
           VHSNRQF+RECDIYGTVDFIFGNAVVVFQSCNIYARKPMP QKNTITAQ+RKDPNQNTGI
Sbjct: 423 VHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQNTGI 482

Query: 482 SIHACKILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD 541
           SIHACKILA +DLE SKGS+PTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD
Sbjct: 483 SIHACKILATTDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD 542

Query: 542 TLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFSAGL 595
           TLYYGEYMNSGPGAA GQRV WPGYRVITST+EA KFTVGQFIYGS+WLPSTGVSFSAGL
Sbjct: 543 TLYYGEYMNSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFSAGL 590

BLAST of MC06g1384 vs. NCBI nr
Match: KAG6605784.1 (putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 916 bits (2368), Expect = 0.0
Identity = 473/601 (78.70%), Postives = 523/601 (87.02%), Query Frame = 0

Query: 2   NYGKLHSTSSGDTPANFELSNSNSSISSPNRRTRTRVVLLSLLSILLIMASAISAVVFVR 61
           NY KLHS++SGDTPA F++S++ +  SS   R RTR++ +SL+SILLI  SA+SA V + 
Sbjct: 3   NYNKLHSSASGDTPATFQVSHTPNPPSSSASRRRTRILFVSLISILLIAVSAVSAAVLIH 62

Query: 62  SHIGRGGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALAADVHDLVHISLNVT 121
           SH      +      KP+QAIS ACS TLYP++C SSL+ FPG+L+ADVH LVHISLN+T
Sbjct: 63  SHTSHFPHN------KPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNLT 122

Query: 122 LQRLTKALYSSSQIPVSQISD-PLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDVLTW 181
           LQ LTKALY+SSQIPV QIS+ PL  SA+ DCIELL+DAVDAFS SLFST+ASNHD++TW
Sbjct: 123 LQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTW 182

Query: 182 LSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPV 241
           LSAALTYHDTC++G ADV    VKDQM A+L+DLS++ISNSLAIFSG+G        +PV
Sbjct: 183 LSAALTYHDTCSDGFADVD-DPVKDQMAARLSDLSEMISNSLAIFSGYG------EELPV 242

Query: 242 QNRRRLMEA--EWPQENGGE----EFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYK 301
           QNRRRLME+  EW  ENG      EFP WVS KDR+LL +PISAIQADIVVAKDG+GK+ 
Sbjct: 243 QNRRRLMESDREWVAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFT 302

Query: 302 TVAEAIAAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFD 361
           TVAEAI AAPSSSGRR VIY+KAGKY EENLKVGRKKTNLMFVGDGKGITVISGGKSVFD
Sbjct: 303 TVAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFD 362

Query: 362 KVTTFRTATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLY 421
           KVTTFRTATFA SGTGIILRDMTFENTAGP +HQAVALRVSADHAVV+RCNIIGYQDTLY
Sbjct: 363 KVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRVSADHAVVYRCNIIGYQDTLY 422

Query: 422 VHSNRQFYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGI 481
           VHSNRQF+RECDIYGTVDFIFGNAVVVFQSCNIYARKPMP QKNTITAQ+RKDPNQNTGI
Sbjct: 423 VHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQNTGI 482

Query: 482 SIHACKILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD 541
           SIHACKILA +DLE SKGS+PTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD
Sbjct: 483 SIHACKILATTDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD 542

Query: 542 TLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFSAGL 595
           TLYYGEYMNSGPGAA GQRV WPGYRVITST+EA KFTVGQFIYGS+WLPSTGVSFSAGL
Sbjct: 543 TLYYGEYMNSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFSAGL 590

BLAST of MC06g1384 vs. NCBI nr
Match: KAG6579321.1 (putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 913 bits (2359), Expect = 0.0
Identity = 473/604 (78.31%), Postives = 530/604 (87.75%), Query Frame = 0

Query: 2   NYGKLHSTSSGDTPAN-FELSNSNSSISSPNR--RTRTRVVLLSLLSILLIMASAISAVV 61
           NY KLHS+ SGDTPA+ F +SN +S    P     +R R++L+S LSI LI ASA+SAVV
Sbjct: 4   NYNKLHSSRSGDTPASSFRVSNDHSDPLPPPSPSSSRRRILLISFLSIFLITASAVSAVV 63

Query: 62  FVRSHIGRGGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALAADVHDLVHISL 121
            V S I    ES     +KP+QAISRACS TLYP++C+SSL+  PG+L+ADVHDLVHISL
Sbjct: 64  LVHSRIAASTESHFP-HNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLVHISL 123

Query: 122 NVTLQRLTKALYSSSQIPVSQIS-DPLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDV 181
           N+TLQ LTKALYS+SQIP+SQIS DPL  SA+ DC+ELL+DAV+AFS SLFST A+NHDV
Sbjct: 124 NLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTATNHDV 183

Query: 182 LTWLSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSG 241
           +TWLSAALTYHDTCT+G +DV A  +KDQ+TAKL+DLS++ISNSLAIFSGFGG       
Sbjct: 184 MTWLSAALTYHDTCTDGFSDV-ADPIKDQITAKLSDLSEMISNSLAIFSGFGGDD----- 243

Query: 242 VPVQNRRR--LMEAE--WPQE--NGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTG 301
           +PV+NRRR  LME++  W  E  NG  +FP+WVS KDR+LL +PIS+IQADIVVAKDG+G
Sbjct: 244 LPVENRRRRRLMESDEHWLAEENNGDGDFPQWVSGKDRRLLAAPISSIQADIVVAKDGSG 303

Query: 302 KYKTVAEAIAAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKS 361
           K+KTVAEAIAAAPSSSGRR VIY+KAGKY EENLKVGRKKTNLMFVGDGKGITVISGGKS
Sbjct: 304 KFKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKS 363

Query: 362 VFDKVTTFRTATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQD 421
           V+DKVTTFRTATFA SGTG+ILRDMTFENTAGP +HQAVALR+SADHAVVFR N+IGYQD
Sbjct: 364 VYDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVIGYQD 423

Query: 422 TLYVHSNRQFYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQN 481
           TLYVHSNRQF+RECDIYGT+DFIFGNA VVFQSCNIYARKPM GQKNTITAQDRKDPNQN
Sbjct: 424 TLYVHSNRQFFRECDIYGTIDFIFGNAAVVFQSCNIYARKPMSGQKNTITAQDRKDPNQN 483

Query: 482 TGISIHACKILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF 541
           TGISIHACKI+A  DLE SKGS+PT+LGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF
Sbjct: 484 TGISIHACKIIATGDLEASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF 543

Query: 542 ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFS 595
           ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITST+EA KFTVGQFIYGS+WLPSTGVSFS
Sbjct: 544 ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFS 600

BLAST of MC06g1384 vs. ExPASy TrEMBL
Match: A0A6J1DY95 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111024627 PE=3 SV=1)

HSP 1 Score: 1173 bits (3035), Expect = 0.0
Identity = 597/597 (100.00%), Postives = 597/597 (100.00%), Query Frame = 0

Query: 1   MNYGKLHSTSSGDTPANFELSNSNSSISSPNRRTRTRVVLLSLLSILLIMASAISAVVFV 60
           MNYGKLHSTSSGDTPANFELSNSNSSISSPNRRTRTRVVLLSLLSILLIMASAISAVVFV
Sbjct: 1   MNYGKLHSTSSGDTPANFELSNSNSSISSPNRRTRTRVVLLSLLSILLIMASAISAVVFV 60

Query: 61  RSHIGRGGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALAADVHDLVHISLNV 120
           RSHIGRGGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALAADVHDLVHISLNV
Sbjct: 61  RSHIGRGGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALAADVHDLVHISLNV 120

Query: 121 TLQRLTKALYSSSQIPVSQISDPLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDVLTW 180
           TLQRLTKALYSSSQIPVSQISDPLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDVLTW
Sbjct: 121 TLQRLTKALYSSSQIPVSQISDPLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDVLTW 180

Query: 181 LSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPV 240
           LSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPV
Sbjct: 181 LSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPV 240

Query: 241 QNRRRLMEAEWPQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAI 300
           QNRRRLMEAEWPQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAI
Sbjct: 241 QNRRRLMEAEWPQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAI 300

Query: 301 AAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFR 360
           AAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFR
Sbjct: 301 AAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFR 360

Query: 361 TATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQ 420
           TATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQ
Sbjct: 361 TATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQ 420

Query: 421 FYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACK 480
           FYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACK
Sbjct: 421 FYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACK 480

Query: 481 ILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGE 540
           ILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGE
Sbjct: 481 ILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGE 540

Query: 541 YMNSGPGAAVGQRVKWPGYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFSAGLSAS 597
           YMNSGPGAAVGQRVKWPGYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFSAGLSAS
Sbjct: 541 YMNSGPGAAVGQRVKWPGYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFSAGLSAS 597

BLAST of MC06g1384 vs. ExPASy TrEMBL
Match: A0A6J1H2K1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459120 PE=3 SV=1)

HSP 1 Score: 922 bits (2382), Expect = 0.0
Identity = 477/602 (79.24%), Postives = 528/602 (87.71%), Query Frame = 0

Query: 2   NYGKLHSTSSGDTPANFELSNSNSSISSPNRRTRTRVVLLSLLSILLIMASAISAVVFVR 61
           NY KLHS++SGDTPA F++S++ +  SS   R RTR++ +SL+SILLI ASA+SA V + 
Sbjct: 3   NYNKLHSSASGDTPATFQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAVLLH 62

Query: 62  SHIGRGGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALAADVHDLVHISLNVT 121
           S      +S     +KP+QAIS ACS TLYP++C SSL+ FPG+L+ADVH LVHISLN+T
Sbjct: 63  S------QSSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNLT 122

Query: 122 LQRLTKALYSSSQIPVSQISD-PLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDVLTW 181
           LQ LTKALY+SSQIPV QIS+ PL  SA+ DCIELL+DAVDAFS SLFST+ASNHD++TW
Sbjct: 123 LQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTW 182

Query: 182 LSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPV 241
           LSAALTYHDTC++G ADV    VKDQM A+L+DLS++ISNSLAIFSG+G       G+PV
Sbjct: 183 LSAALTYHDTCSDGFADVD-DPVKDQMAARLSDLSEMISNSLAIFSGYG------EGLPV 242

Query: 242 QNRRRLMEA--EWPQENGGE----EFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYK 301
           QNRRRLME+  EW  ENG      EFP WVS KDR+LL +PISAIQADIVVAKDG+GK+K
Sbjct: 243 QNRRRLMESDREWAAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFK 302

Query: 302 TVAEAIAAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFD 361
           TVAEAI AAPSSSGRR VIY+KAGKY EENLKVGRKKTNLMFVGDGKGITVISGGKSVFD
Sbjct: 303 TVAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFD 362

Query: 362 KVTTFRTATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLY 421
           KVTTFRTATFA SGTGIILRDMTFENTAGP +HQAVALRVSADHAVV+RCNIIGYQDTLY
Sbjct: 363 KVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRVSADHAVVYRCNIIGYQDTLY 422

Query: 422 VHSNRQFYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGI 481
           VHSNRQF+RECDIYGTVDFIFGNAVVVFQSCNIYARKPMP QKNTITAQ+RKDPNQNTGI
Sbjct: 423 VHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQNTGI 482

Query: 482 SIHACKILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD 541
           SIHACKILA +DLE SKGS+PTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD
Sbjct: 483 SIHACKILATTDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD 542

Query: 542 TLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFSAGL 596
           TLYYGEYMNSGPGAA GQRV WPGYRVITST+EA KFTVGQFIYGS+WLPSTGVSFSAGL
Sbjct: 543 TLYYGEYMNSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFSAGL 591

BLAST of MC06g1384 vs. ExPASy TrEMBL
Match: A0A6J1K6C1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111491552 PE=3 SV=1)

HSP 1 Score: 910 bits (2352), Expect = 0.0
Identity = 472/604 (78.15%), Postives = 523/604 (86.59%), Query Frame = 0

Query: 2   NYGKLHSTSSGDTPANFELSNSNSSISSPNR---RTRTRVVLLSLLSILLIMASAISAVV 61
           NY KLHS+ SGDTPA F++S++ +    P+    R RTR++ +SL+SILLI ASA+SA V
Sbjct: 3   NYNKLHSSGSGDTPATFQVSHTPNPPHPPSSSASRRRTRILFVSLISILLITASAVSAAV 62

Query: 62  FVRSHIGRGGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALAADVHDLVHISL 121
            + SH      +      KP+QAIS ACSRTLYP++C SSL+ FPG+L+ADVH LVHISL
Sbjct: 63  LIHSHSSHIPHN------KPTQAISHACSRTLYPSLCRSSLLSFPGSLSADVHHLVHISL 122

Query: 122 NVTLQRLTKALYSSSQIPVSQISD-PLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDV 181
           N+TLQ LTKALY+SSQIPV QIS+ PL  SA+ DCIELL+DAVDAFS SLFST+ASNHD+
Sbjct: 123 NLTLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDI 182

Query: 182 LTWLSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSG 241
           +TWLSAALTYHDTC++G ADV    VKDQM ++L+DLS++ISNSLAIFSG+G       G
Sbjct: 183 MTWLSAALTYHDTCSDGFADVD-DPVKDQMASRLSDLSEMISNSLAIFSGYG------EG 242

Query: 242 VPVQNRRRLMEA--EWPQENGGE----EFPEWVSRKDRQLLGSPISAIQADIVVAKDGTG 301
           +PVQNRRRLME+  EW  ENG      EFP WVS KDR+LL + ISAIQADIVVAKDG+G
Sbjct: 243 LPVQNRRRLMESDREWAAENGVAVAEGEFPGWVSGKDRRLLAASISAIQADIVVAKDGSG 302

Query: 302 KYKTVAEAIAAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKS 361
           K+KTVAEAI AAPSSSGRR VIY+KAGKY EENLKVGRKKTNLMFVGDGKGITVISGGKS
Sbjct: 303 KFKTVAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKS 362

Query: 362 VFDKVTTFRTATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQD 421
           VFDKVTTFRTATFA SGTGIILRDMT ENTAGP +HQAVALRVSADHAVV+RCNIIGYQD
Sbjct: 363 VFDKVTTFRTATFAGSGTGIILRDMTIENTAGPSKHQAVALRVSADHAVVYRCNIIGYQD 422

Query: 422 TLYVHSNRQFYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQN 481
           TLYVHSNRQF+RECDIYGTVDFIFGNAVVVFQSCNIYARKPMP QKNTITAQ+RKDPNQN
Sbjct: 423 TLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQN 482

Query: 482 TGISIHACKILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF 541
           TGISIHACKILA +DLE SKGS+ TFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF
Sbjct: 483 TGISIHACKILATTDLEASKGSFSTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF 542

Query: 542 ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFS 595
           ALDTLYYGEYMNSGPGAA GQRV WPGYRVITST+EA KFTVGQFIYGS+WLPSTGVSFS
Sbjct: 543 ALDTLYYGEYMNSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFS 593

BLAST of MC06g1384 vs. ExPASy TrEMBL
Match: A0A6J1E2V1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430323 PE=3 SV=1)

HSP 1 Score: 908 bits (2347), Expect = 0.0
Identity = 471/604 (77.98%), Postives = 528/604 (87.42%), Query Frame = 0

Query: 2   NYGKLHSTSSGDTPAN-FELSNSNSSISSPNR--RTRTRVVLLSLLSILLIMASAISAVV 61
           NY  LHS+ SGDTPA+ F +SN +S    P     +R R++L+S  SILLI ASA+SAVV
Sbjct: 4   NYNMLHSSHSGDTPASSFRVSNDHSDPLPPPSPSSSRRRILLISFFSILLITASAVSAVV 63

Query: 62  FVRSHIGRGGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALAADVHDLVHISL 121
            V S I    ES     +KP+QAISRACS TLYP++C+SSL+  PG+L+ADVHDLVHISL
Sbjct: 64  LVHSRIAASTESHFP-HNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLVHISL 123

Query: 122 NVTLQRLTKALYSSSQIPVSQIS-DPLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDV 181
           N+TLQ LTKALYS+SQIP+SQIS DPL  SA+ DC+ELL+DAV+AFS SLFST A+NHDV
Sbjct: 124 NLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTATNHDV 183

Query: 182 LTWLSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSG 241
           +TWLSAALTYHDTCT+G +DV A  +KDQ+TAKL+DLS++ISNSLAIFSGFGG       
Sbjct: 184 MTWLSAALTYHDTCTDGFSDV-ADPIKDQITAKLSDLSEMISNSLAIFSGFGGDD----- 243

Query: 242 VPVQNRRR--LMEAE--WPQE--NGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTG 301
           +PV+NRRR  LME++  W  E  NG  +FP WVS KDR+LL +PIS+IQADIVVAKDG+G
Sbjct: 244 LPVENRRRRRLMESDEQWLAEENNGDGDFPRWVSGKDRRLLAAPISSIQADIVVAKDGSG 303

Query: 302 KYKTVAEAIAAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKS 361
           K+KTVAEAIAAAPSSSGRR VIY+KAGKY EENLKVGRKKTNLMFVGDGKGITVISGGKS
Sbjct: 304 KFKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKS 363

Query: 362 VFDKVTTFRTATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQD 421
           V+DKVTTFRTATFA SGTG+ILRDMTFENTAGP +HQAVALR+SADHAVVFR N+IGYQD
Sbjct: 364 VYDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVIGYQD 423

Query: 422 TLYVHSNRQFYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQN 481
           TLYVHSNRQF+RECDIYGT+DFIFGNAVVVFQSCNIYARKPM GQKNTITAQDRKDPNQN
Sbjct: 424 TLYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMSGQKNTITAQDRKDPNQN 483

Query: 482 TGISIHACKILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF 541
           TGISIHACKI+A  DL+ SKGS+PT+LGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF
Sbjct: 484 TGISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF 543

Query: 542 ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFS 595
           ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITS +EA KFTVGQFIYGS+WLPSTGVSFS
Sbjct: 544 ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSAIEATKFTVGQFIYGSSWLPSTGVSFS 600

BLAST of MC06g1384 vs. ExPASy TrEMBL
Match: A0A6J1IA52 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111471502 PE=3 SV=1)

HSP 1 Score: 901 bits (2329), Expect = 0.0
Identity = 467/604 (77.32%), Postives = 523/604 (86.59%), Query Frame = 0

Query: 2   NYGKLHSTSSGDTPA-NFELSN--SNSSISSPNRRTRTRVVLLSLLSILLIMASAISAVV 61
           NY KLHS+ SGDTP  +F +SN  SN     P   +  R++L+S  SILLI AS +SAVV
Sbjct: 4   NYNKLHSSRSGDTPVTSFRVSNDHSNPPPPPPPSSSHRRILLISFFSILLITASTVSAVV 63

Query: 62  FVRSHIGRGGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALAADVHDLVHISL 121
            V S I    ES     +KP+QAISRACS TLYP++C+SSL+  PG+L+ADVHDLVHISL
Sbjct: 64  LVHSRITTSTESHFP-HNKPTQAISRACSLTLYPSLCISSLLSAPGSLSADVHDLVHISL 123

Query: 122 NVTLQRLTKALYSSSQIPVSQIS-DPLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDV 181
           N+TLQ LTKALYS+SQIP+SQIS DPL  SA+ DC+ELL+DAV+AFS SLFST A+NHDV
Sbjct: 124 NLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTATNHDV 183

Query: 182 LTWLSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSG 241
           +TWLSAALTYHDTCT+G +DV A  VKDQ+TAKL+DLS++ISNSLAIFSGFGGG      
Sbjct: 184 MTWLSAALTYHDTCTDGFSDV-ADPVKDQITAKLSDLSEMISNSLAIFSGFGGGD----- 243

Query: 242 VPVQNRRR--LMEAE--WPQE--NGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTG 301
           +PV+NRRR  LME++  W  E  NG  +FP WVS KDR+LL +PIS+IQADIVVAKDG+G
Sbjct: 244 LPVENRRRRRLMESDEQWLAEENNGDGDFPRWVSGKDRRLLAAPISSIQADIVVAKDGSG 303

Query: 302 KYKTVAEAIAAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKS 361
           K+KTVAEAIAAAPSSS RR VIY+KAGKY EENLKVGRKKTNLMFVGDGKGITVISGGKS
Sbjct: 304 KFKTVAEAIAAAPSSSSRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKS 363

Query: 362 VFDKVTTFRTATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQD 421
           V+DKVTTFRTATFA SGTG+ILRDMTFENTAGP +HQAVALR+SADH VVFR N+IGYQD
Sbjct: 364 VYDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHTVVFRSNVIGYQD 423

Query: 422 TLYVHSNRQFYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQN 481
           TLYVHSNRQF+RECDIYGT+DFIFGNA VVFQSCNIYARKPM GQKNTITAQDRKDPNQN
Sbjct: 424 TLYVHSNRQFFRECDIYGTIDFIFGNAAVVFQSCNIYARKPMSGQKNTITAQDRKDPNQN 483

Query: 482 TGISIHACKILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF 541
           TGISIHACKI+A  DL+ SKGS+PT+LGRPWKLYSRVVYMVSS+GDHIHPRGWLEWQGSF
Sbjct: 484 TGISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSLGDHIHPRGWLEWQGSF 543

Query: 542 ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFS 595
           ALDTLYYGEYMNSGP AAVGQRVKWPGYRVITS +EA KFTVGQFIYGS+WLPSTGVSFS
Sbjct: 544 ALDTLYYGEYMNSGPSAAVGQRVKWPGYRVITSAIEATKFTVGQFIYGSSWLPSTGVSFS 600

BLAST of MC06g1384 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 723.4 bits (1866), Expect = 1.5e-208
Identity = 365/588 (62.07%), Postives = 452/588 (76.87%), Query Frame = 0

Query: 15  PANFELSNSNSSISSPNRRTRTRVVLLSLLSILLIMASAISAVVFVRSHIGRGGESGLGL 74
           P+N    +  +S+    ++T+T+++L +L    +++   +   +F        G++   L
Sbjct: 11  PSNPNQKDPATSLPELQKKTKTKLILFTL---AVLVVGVVCFGIFAGIRAVDSGKTEPKL 70

Query: 75  AHKPSQAISRACSRTLYPAVCVSSLVEFPGALAADVHDLVHISLNVTLQRLTKALYSSSQ 134
             KP+QAISR CS++LYP +C+ +L++FPG+L AD ++L+HIS N TLQ+ +KALY+SS 
Sbjct: 71  TRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSST 130

Query: 135 IPVSQISDPLLLSAFADCIELLDDAVDAFSRSLFSTAA-----SNHDVLTWLSAALTYHD 194
           I  +Q+  P + SA+  C+ELLDD+VDA +R+L S        S+ DV+TWLS+A+T HD
Sbjct: 131 ITYTQM-PPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTNHD 190

Query: 195 TCTEGLADV--SAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPVQNRRRLM 254
           TCT+G  ++    G+VKDQ+   + DLS+++SN LAIF+   G   + SGVPV N R+L+
Sbjct: 191 TCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFA---GKVKDLSGVPVVNNRKLL 250

Query: 255 EAEWPQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAIAAAPSSS 314
             E       EE P W+ R+DR+LLG+P SAIQADI V+KDG+G +KT+AEAI  AP  S
Sbjct: 251 GTE-----ETEELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHS 310

Query: 315 GRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAAS 374
            RR VIYVKAG+Y EENLKVGRKKTNLMF+GDGKG TVI+GGKS+ D +TTF TATFAA+
Sbjct: 311 SRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAAT 370

Query: 375 GTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQFYRECDI 434
           G G I+RDMTFEN AGP +HQAVALRV  DHAVV+RCNIIGYQD LYVHSNRQF+REC+I
Sbjct: 371 GAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEI 430

Query: 435 YGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKILAASDL 494
           YGTVDFIFGNA V+ QSCNIYARKPM  QK TITAQ+RKDPNQNTGISIHACK+LA  DL
Sbjct: 431 YGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDL 490

Query: 495 EGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPG 554
           E SKGSYPT+LGRPWKLYSRVVYM+S MGDHI PRGWLEW G FALD+LYYGEYMN G G
Sbjct: 491 EASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLG 550

Query: 555 AAVGQRVKWPGYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFSAGLS 596
           + +GQRVKWPGY VITSTVEA KFTV QFI GS+WLPSTGVSF +GLS
Sbjct: 551 SGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

BLAST of MC06g1384 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 701.4 bits (1809), Expect = 6.1e-202
Identity = 368/593 (62.06%), Postives = 453/593 (76.39%), Query Frame = 0

Query: 8   STSSGDTPANFELSNSNSSISSPNRRTRTRVVLLSL-LSILLIMASAISAVVFVRSHIGR 67
           +TS+   P   ++S S     + NRR++ ++V+ S+ L+I LI+A+AI A V  R  +  
Sbjct: 16  TTSTTTAPILNQVSTSEQP-ENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKL-- 75

Query: 68  GGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALAA-DVHDLVHISLNVTLQRL 127
             +S  GLA KPSQAIS+AC  T +P +CV SL++FPG+LAA    DL+H+++N+TL   
Sbjct: 76  -NQSVPGLARKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHF 135

Query: 128 TKALYSSSQIPVSQISDPLLLSAFADCIELLDDAVDAFSRSLFSTAASN---HDVLTWLS 187
           + ALYSS+ +    +  P   SA+  C+ELLDD+VDA SR+L S  +S+    DV TWLS
Sbjct: 136 SHALYSSASLSFVDM-PPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLS 195

Query: 188 AALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPVQN 247
           AALT HDTCTEG   V  G VKD MTA L +LS+L+SN LAIFS    G  +F+GVP+QN
Sbjct: 196 AALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDG-DDFAGVPIQN 255

Query: 248 RRRLMEAEWPQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAIAA 307
           RR L       E   E+FP W+  K+R++L  P+S IQADI+V+KDG G  KT++EAI  
Sbjct: 256 RRLL-----GVEEREEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKK 315

Query: 308 APSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTA 367
           AP +S RR +IYVKAG+Y E NLKVGRKK NLMFVGDGKG TVISGGKS+FD +TTF TA
Sbjct: 316 APQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTA 375

Query: 368 TFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQFY 427
           +FAA+G G I RD+TFEN AGP +HQAVALR+ ADHAV++RCNIIGYQDTLYVHSNRQF+
Sbjct: 376 SFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFF 435

Query: 428 RECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKIL 487
           RECDIYGTVDFIFGNA VV Q+C+IYARKPM  QKNTITAQ+RKDPNQNTGISIHA ++L
Sbjct: 436 RECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVL 495

Query: 488 AASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYM 547
           AASDL+ + GS  T+LGRPWKL+SR VYM+S +G H+H RGWLEW  +FALDTLYYGEY+
Sbjct: 496 AASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYL 555

Query: 548 NSGPGAAVGQRVKWPGYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFSAGLS 596
           NSGPG+ +GQRV WPGYRVI ST EA +FTV +FIYGS+WLPSTGVSF AGLS
Sbjct: 556 NSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597

BLAST of MC06g1384 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 433.7 bits (1114), Expect = 2.4e-121
Identity = 252/591 (42.64%), Postives = 352/591 (59.56%), Query Frame = 0

Query: 28  SSPNRRTRTRVVLLSLLSILLIMASAISAVVFVRSHIGRGGESGLGLAHKPSQAISRACS 87
           S  N +   ++VLLS    LL +A    AV  + +   +  E    L+      +  +CS
Sbjct: 10  SKDNFKKNKKLVLLSAAVALLFVA----AVAGISAGASKANEKRT-LSPSSHAVLRSSCS 69

Query: 88  RTLYPAVCVSSLVEFPGALAADVHDLVHISLNVTLQRLTKALYSSSQ-IPVSQISDPLLL 147
            T YP +C+S++V   G       D++  S+N+T+  +    ++  + I   +   P   
Sbjct: 70  STRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREK 129

Query: 148 SAFADCIELLDDAVDAFSRS-----LFSTAAS--NH--DVLTWLSAALTYHDTCTEGLA- 207
           +A  DC+E +D+ +D    +     L+ T  +   H  D+ T +S+A+T  +TC +G + 
Sbjct: 130 TALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSH 189

Query: 208 DVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGG---GTGEFSGVPVQNRRRLMEAEWPQ 267
           D +  QV+  +      +  + SN+LA+            E       N R+L E     
Sbjct: 190 DDADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQET 249

Query: 268 ----------ENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAIAAA 327
                     E   E +P W+S  DR+LL    S ++AD  VA DG+G +KTVA A+AAA
Sbjct: 250 TVAVDIAGAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKTVAAAVAAA 309

Query: 328 PSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTAT 387
           P +S +R VI++KAG Y  EN++V +KK N+MF+GDG+  T+I+G ++V D  TTF +AT
Sbjct: 310 PENSNKRYVIHIKAGVY-RENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSAT 369

Query: 388 FAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQFYR 447
            AA G   + RD+TF+NTAGP +HQAVALRV +D +  + C+++ YQDTLYVHSNRQF+ 
Sbjct: 370 VAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFV 429

Query: 448 ECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKILA 507
           +C I GTVDFIFGNA VV Q C+I+AR+P  GQKN +TAQ R DPNQNTGI I  C+I A
Sbjct: 430 KCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGA 489

Query: 508 ASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMN 567
            SDL+  KGS+PT+LGRPWK YS+ V M S++ D I P GW EW G+FAL+TL Y EY N
Sbjct: 490 TSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSN 549

Query: 568 SGPGAAVGQRVKWPGYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFSAGL 595
           +G GA    RVKW G++VIT+  EA K+T GQFI G  WL STG  FS GL
Sbjct: 550 TGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of MC06g1384 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 430.6 bits (1106), Expect = 2.0e-120
Identity = 247/573 (43.11%), Postives = 341/573 (59.51%), Query Frame = 0

Query: 48  LIMASAISAVVFVRSHIG-------RGGESGLGLAHKPSQAISRA-CSRTLYPAVCVSSL 107
           LI++SA  A++ + S +G       +     +      S AI ++ CS TLYP +C S++
Sbjct: 20  LILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPELCFSAV 79

Query: 108 VEFPGALAADVHDLVHISLNVTLQRLTKALYSSSQIPVSQIS-DPLLLSAFADCIELLDD 167
               G       +++  SLN+T + +    ++  ++   +    P  ++A  DC+E +D+
Sbjct: 80  AATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDCLETIDE 139

Query: 168 AVDAFSRSL---------FSTAASNHDVLTWLSAALTYHDTCTEGLA-DVSAGQVKDQMT 227
            +D    ++          S      D+ T +S+A+T   TC +G + D +  +V+  + 
Sbjct: 140 TLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRKVRKALL 199

Query: 228 AKLADLSQLISNSLAIFSGFGGG-------TGEFSGVPVQNRRRLMEAEWPQENGGEEFP 287
                +  + SN+LA+                + S     N R+L E     ++ G  +P
Sbjct: 200 KGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGDLDSDG--WP 259

Query: 288 EWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAIAAAPSSSGRRTVIYVKAGKYV 347
           +W+S  DR+LL    S I+AD  VA DG+G + TVA A+AAAP  S +R VI++KAG Y 
Sbjct: 260 KWLSVGDRRLLQG--STIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIHIKAGVY- 319

Query: 348 EENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAASGTGIILRDMTFENT 407
            EN++V +KKTN+MF+GDG+G T+I+G ++V D  TTF +AT AA G   + RD+TF+NT
Sbjct: 320 RENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLARDITFQNT 379

Query: 408 AGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQFYRECDIYGTVDFIFGNAVVV 467
           AGP +HQAVALRV +D +  ++C++  YQDTLYVHSNRQF+ +C I GTVDFIFGNA  V
Sbjct: 380 AGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFIFGNAAAV 439

Query: 468 FQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKILAASDLEGSKGSYPTFLGRP 527
            Q C+I AR+P  GQKN +TAQ R DPNQNTGI I  C+I   SDL   KG++PT+LGRP
Sbjct: 440 LQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTFPTYLGRP 499

Query: 528 WKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRVKWPGYRV 587
           WK YSR V M S + D I P GW EW GSFALDTL Y EY+N G GA    RVKW GY+V
Sbjct: 500 WKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRVKWKGYKV 559

Query: 588 ITSTVEAGKFTVGQFIYGSAWLPSTGVSFSAGL 595
           ITS  EA  FT GQFI G  WL STG  FS  L
Sbjct: 560 ITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of MC06g1384 vs. TAIR 10
Match: AT1G11580.1 (methylesterase PCR A )

HSP 1 Score: 429.1 bits (1102), Expect = 5.8e-120
Identity = 257/587 (43.78%), Postives = 348/587 (59.28%), Query Frame = 0

Query: 20  LSNSNSSISSPNRRTRTRVVLLSLLSILLIMASAISAVVFVRSHIGRGGESGLGLAHKPS 79
           +SNSN  + S  +  + + + L L  + ++ + A      +  +     +S L       
Sbjct: 1   MSNSNQPLLSKPKSLKHKNLCLVLSFVAILGSVAFFTAQLISVNTNNNDDSLL------- 60

Query: 80  QAISRACSRTLYPAVCVSSLVEFPGALAADVH--DLVHISLNVTLQRL--TKALYSSSQI 139
              S+ C        C + L EF     + ++  DL+H+ L  ++ RL  T  + S ++I
Sbjct: 61  -TTSQICHGAHDQDSCQALLSEFTTLSLSKLNRLDLLHVFLKNSVWRLESTMTMVSEARI 120

Query: 140 PVSQISDPLLLSAFADCIELLDDAVDAFSRSLFSTAASNH------DVLTWLSAALTYHD 199
             + + D    + FADC E++D + D    S+      N+      +V TWLS+ LT + 
Sbjct: 121 RSNGVRDK---AGFADCEEMMDVSKDRMMSSMEELRGGNYNLESYSNVHTWLSSVLTNYM 180

Query: 200 TCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPVQNRRRLMEA 259
           TC E ++DVS    K  +  +L DL      +LAIF          S +P ++  +++ +
Sbjct: 181 TCLESISDVSVNS-KQIVKPQLEDLVSRARVALAIF---------VSVLPARDDLKMIIS 240

Query: 260 EWPQENGGEEFPEWVSRKDRQLLGSPISAIQ--ADIVVAKDGTGKYKTVAEAIAAAPSSS 319
                     FP W++  DR+LL S    ++  A++VVAKDGTGK+KTV EA+AAAP +S
Sbjct: 241 --------NRFPSWLTALDRKLLESSPKTLKVTANVVVAKDGTGKFKTVNEAVAAAPENS 300

Query: 320 GRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAAS 379
             R VIYVK G Y +E + +G+KK NLM VGDGK  T+I+G  +V D  TTFR+AT AA+
Sbjct: 301 NTRYVIYVKKGVY-KETIDIGKKKKNLMLVGDGKDATIITGSLNVIDGSTTFRSATVAAN 360

Query: 380 GTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQFYRECDI 439
           G G + +D+ F+NTAGP +HQAVALRVSAD  V+ RC I  YQDTLY H+ RQFYR+  I
Sbjct: 361 GDGFMAQDIWFQNTAGPAKHQAVALRVSADQTVINRCRIDAYQDTLYTHTLRQFYRDSYI 420

Query: 440 YGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKILAASDL 499
            GTVDFIFGN+ VVFQ+C+I AR P  GQKN +TAQ R+D NQNT ISI  CKI A+SDL
Sbjct: 421 TGTVDFIFGNSAVVFQNCDIVARNPGAGQKNMLTAQGREDQNQNTAISIQKCKITASSDL 480

Query: 500 EGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPG 559
              KGS  TFLGRPWKLYSR V M S + +HI P GW  W G FAL TLYYGEY N+GPG
Sbjct: 481 APVKGSVKTFLGRPWKLYSRTVIMQSFIDNHIDPAGWFPWDGEFALSTLYYGEYANTGPG 540

Query: 560 AAVGQRVKWPGYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFSAGL 595
           A   +RV W G++VI  + EA +FTV + I G  WL  TGV+F   L
Sbjct: 541 ADTSKRVNWKGFKVIKDSKEAEQFTVAKLIQGGLWLKPTGVTFQEWL 557